CN110464361A - A kind of method that enzymatic reaction system shows for latent fingerprint - Google Patents
A kind of method that enzymatic reaction system shows for latent fingerprint Download PDFInfo
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- CN110464361A CN110464361A CN201910722660.3A CN201910722660A CN110464361A CN 110464361 A CN110464361 A CN 110464361A CN 201910722660 A CN201910722660 A CN 201910722660A CN 110464361 A CN110464361 A CN 110464361A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/117—Identification of persons
- A61B5/1171—Identification of persons based on the shapes or appearances of their bodies or parts thereof
- A61B5/1172—Identification of persons based on the shapes or appearances of their bodies or parts thereof using fingerprinting
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Abstract
The present invention relates to trace detection technical fields, specifically disclose a kind of method that enzymatic reaction system shows for latent fingerprint, the method that this method shows latent fingerprint for a kind of enzymic catalytic reaction generation fluorescence-causing substance.First prepare enzymatic reaction system developing solution, then sprayed or be coated onto may have fingerprint but without fluorescent material fingerprint object on, the substances such as the enzyme in system can react with the lactic acid in fingerprint, there is the product of fluorescence to show fingerprint, can directly take pictures under ultraviolet light, the advantage of the invention is that sensitive, quick, colour reagent composition is uncomplicated, toxicity will not be generated to operator, can also realize " hidden scouting ", be expected in terms of be applied.
Description
Technical field
The present invention relates to trace detection technical fields, and in particular to what a kind of enzymatic reaction system showed for latent fingerprint
Method.
Background technique
The characteristics of fingerprint has people variant, constant throughout one's life and touching object trace.Science correctly finds, extracts, showing, reflecting
Determine fingerprint for carrying out investigation, punishing crime with important practice effect, to the fingerprint being retained on object surface into
Row extracts and identification is the important evidence of clear up a criminal case.
The method of identification latent fingerprint can be divided into three categories at present: physics appearance method, physical chemistry appearance method and chemistry are aobvious
Existing method, the development and improvement of current techniques are concentrated mainly on using novel characterization method the sensitivity for increasing inspection and not
With the scope of application, in Chinese patent (CN201410146802.3), Wang Lihua etc. uses the progress of a variety of fluorescent quenching systems
Latent fingerprint shows.In Chinese patent (CN201810189499.3), inventor is prepared for a kind of magnetic fluorescence powder progress
Developing latent finger printss improve sensitivity, but its preparation process is complicated.Chinese patent (CN201710888373.0) is prepared for one kind
Special material: black body radiation luminescent material is reduced background fluorescence by the material and obtains more visible fingerprint image, but should
The preparation process of material is complicated.
Enzyme is catalyzed reaction efficiency height, specificity is strong, high sensitivity as biocatalyst.Chinese patent
(CN201710045379.1) latent fingerprint is shown using enzyme, but its needs shifts latent sample fingerprint twice,
After being marked again with antibody, just shown with related reagent, whole operation process is extremely complex, and time-consuming.
It has not yet to see at normal temperatures and pressures, is reacted in the short time by trace materials in enzymatic latent fingerprint aobvious
The now report of latent fingerprint.
Summary of the invention
For the deficiencies in the prior art, the object of the present invention is to provide a kind of enzymatic reaction systems for diving
In the method for fingerprint manifestation.Sweat is the main component of latent fingerprint, in fingerprint sweat other than most moisture, is also contained
The substances such as lactic acid, amino acid, protein, sodium chloride, documents and materials show that lactic acid content is about 6.6 × 10 in sweat-3mol/L。
Lactic dehydrogenase, which can be catalyzed lactic acid and react, generates pyruvic acid, and the lactic acid detection of the reaction is limited to 2.25 × 10-5mol/L；
Since enzymatic reaction has specificity, other Coexisting components will not generate interference to the reaction in latent fingerprint, therefore use the party
Method can directly be measured the lactic acid in latent fingerprint.It can establish using the enzymatic reaction a kind of at low cost, quick, sensitive
Degree height, developing latent finger printss method easy to operate.This method can quickly show invisible fingerprints of sweat and show reagent composition it is uncomplicated,
Show easy to operate, toxicity will not be generated to operator.
In order to achieve the above technical purposes, the method that a kind of enzymatic reaction system of the invention shows for latent fingerprint
Steps are as follows:
(1) enzymatic reaction system developing solution is prepared, it is spare to be placed in refrigerator；
(2) enzymatic reaction system developing solution is sprayed or be coated onto may have fingerprint but without fluorescent material fingerprint object on；
(3) develop under ultraviolet lighting.
The enzymatic reaction system developing solution includes: 50~80U/mL lactic dehydrogenase, 0.007~0.009mol/L oxidation
Three kinds of liquid of type nicotinamide adenine dinucleotide and Tris-HCl- hydrazine hydrate buffer.
The preparation method of the Tris-HCl- hydrazine hydrate buffer: referring to the annex in conventional Biochemistry Experiment book
Configure pH9.0,0.05mol/LTris-HCl buffer after, then thereto be added hydrazine hydrate make hydrazine hydrate final concentration of 1.2~
1.8mol/L (preferably 1.5mol/L)；
The hydrazine hydrate is added in the form of the hydrazine hydrate solution that concentration of hydrazine hydrate is 50% (mass percent) or more.
Sequence enzymatic reaction system developing solution spray or be coated on fingerprint object are as follows: first spray or apply Tris-HCl- water
Hydrazine buffer and oxidized nicotinamide adenine dinucleotide are closed, finally spray or applies lactic dehydrogenase, wherein Tris-HCl- is hydrated
Hydrazine buffer and oxidized nicotinamide adenine dinucleotide sequencing are unlimited, can also spray or apply simultaneously.
Preferably, the compound of appropriate water-soluble poor fluidity can also be added in the enzymatic reaction system developing solution,
Such as glycerol, polyethylene glycol 400 (PEG400), the viscosity of Lai Zengjia system, it is apparent to make to show result, substitutes part water,
It is added in Tris-HCl- hydrazine hydrate buffer.
Preferably, the fingerprint is invisible fingerprints of sweat.
Preferably, the fingerprint object is writing paper, brown paper, filter paper, art paper, the newspaper etc. of unstressed configuration ingredient.
That the reaction mechanism is as follows is described for enzymatic reaction system of the present invention:
Lactic acid is in oxidized nicotinamide adenine dinucleotide (NAD+) it is existing under the conditions of, by lactic dehydrogenase (LDH)
Catalysis generates pyruvic acid and reduced nicotinamide adenine dinucleotide (NADH).Tris-HCl- water is added in the reaction system
The pyruvic acid that closing hydrazine buffer can be such that reaction generates continues to convert, and makes to detect the lactic acid fully reacting in sample.What reaction generated
NADH is a kind of hyperfluorescence substance, and substrate NAD+Unstressed configuration, therefore lactic acid can be judged from scratch by fluorescence signal
It whether there is, further, the change rate of measurement NADH fluorescent value can calculate the lactic acid content in sample.
Compared with prior art, it the advantages of the method for the present invention and has the beneficial effect that:
1, invisible fingerprints of sweat can quickly be shown；Show easy to operate.
2, it is uncomplicated to show reagent composition, it is at low cost；Toxicity will not be generated to operator.
3, reaction will not form coloring matter, can only be under the irradiation of the light of special wavelength just it is observed that invisible fingerprints of sweat, In
It can't see the exception on article under natural light or common light, it is expected to realize hidden investigation, be expected in developing latent finger printss such as criminal investigations
Aspect is applied.
Detailed description of the invention
Fig. 1 is the fingerprint image that embodiment 1 shows on writing paper, and wherein Fig. 1 (a) is the ultraviolet excitation of 365nm wavelength
Afterwards, when there is no optical filter, visually see that bias light is stronger；After Fig. 1 (b) is the ultraviolet excitation of 365nm wavelength, there is optical filter
When, visually see that bias light interference is weaker.
Fig. 2 is the fingerprint image that embodiment 2 shows on writing paper.
Fig. 3 is the fingerprint image that embodiment 3 shows on writing paper.
Fig. 4 is the fingerprint image that embodiment 4 shows on brown paper.
Fig. 5 is the fingerprint image that embodiment 5 shows on filter paper.
Specific embodiment
Combined with specific embodiments below, technical solution provided by the present invention is described in detail:
NAD+For β-nicotinamide adenine dinucleotide, it is purchased from Beijing Suo Laibao Biotechnology Co., Ltd, BR, 1g, powder
End.No. CAS: 53-84-9.
Lactic dehydrogenase (rabbit flesh), BR, 30U/mg, powder are purchased from Shanghai Yuan Ye Biotechnology Co., Ltd.No. CAS:
9001-60-9。
Embodiment 1
A kind of method that enzymatic reaction system shows for latent fingerprint, its step are as follows:
1, natural fingerprint sample preparation: the experimenter that please provide fingerprint cleans up hand, from doing, then puts on plastic glove,
It seals sweat and press...withes one's finger after ten minutes and be made on the writing paper of unstressed configuration ingredient, use that (simulation criminal investigation is handled a case after placing 4 hours
Actual conditions).
2, developing solution is prepared:
1) Tris-HCl- hydrazine hydrate buffer (wherein concentration of hydrazine hydrate is 1.5mol/L): to 25mL0.2mol/LTris
5mL0.1mol/LHCl solution is added in solution, adds water to 100mL to get the Tris-HCl buffering that pH is 9.0,0.05mol/L
Liquid, then the hydrazine hydrate solution of addition 9.38mL85% (mass percent, similarly hereinafter) is into the buffer up to concentration of hydrazine hydrate
The Tris-HCl- hydrazine hydrate buffer of 1.5mol/L.
2)0.007mol/LNAD+: by 0.046gNAD+It is added in 10mL distilled water to obtain the final product.
3) 50U/mL lactic dehydrogenase: 10mg lactic dehydrogenase is dissolved with 6mL distilled water to obtain the final product.
After each solution prepares, it is spare to be placed in refrigerator.
3, show: successively dipping Tris-HCl- hydrazine hydrate buffer, 0.007mol/LNAD with fine, soft fur brush+With 50U/mL cream
To smearing at the fingerprint of step 1 sample preparation, smearing carries out acidohydrogenase at same direction, does not smear repeatedly and a small amount of, slightly
After drying, is excited with the ultraviolet light irradiation of 365nm wavelength, have fingerprint manifestation, photograph to record.In order to eliminate bias light interference, also
It can assist finding fingerprint by 450nm optical filter.
It will be seen from figure 1 that partial fingerprints lines can be shown on writing paper, wherein (a) is the purple of 365nm wavelength
After outer light excitation, when there is no optical filter, visually see that bias light is stronger；(b) after for the ultraviolet excitation of 365nm wavelength, there is filter
When mating plate, visually see that bias light interference is weaker.
Embodiment 2
A kind of enzymatic reaction system of glycerol adding is used for the method that latent fingerprint shows, step with embodiment 1 operation,
The preparation of buffer is different unlike unique.
The present embodiment use Tris-HCl- glycerol-hydrazine hydrate buffer (wherein concentration of hydrazine hydrate is 1.5mol/L): to
In 25mL0.2mol/LTris solution be added 5mL0.1mol/LHCl solution, add 20mL glycerol, add water to 100mL to get
PH9.0,0.05mol/LTris-HCl- glycerol buffer, then the hydrazine hydrate solution of addition 9.38mL85% is into the buffer
Obtain Tris-HCl- glycerol-hydrazine hydrate buffer that concentration of hydrazine hydrate is 1.5mol/L.
Figure it is seen that clearly fingerprint lines can be shown on writing paper.
Embodiment 3
A method of plus the enzymatic reaction system of PEG400 shows for latent fingerprint, behaviour of the step with embodiment 1
Make, it is unique the difference is that the preparation of buffer is different.
The present embodiment use Tris-HCl-PEG- hydrazine hydrate buffer (wherein concentration of hydrazine hydrate is 1.5mol/L): to
In 25mL0.2mol/LTris solution be added 5mL0.1mol/LHCl solution, add 15mLPEG400, add water to 100mL to get
PH9.0,0.05mol/LTris-HCl-PEG buffer, then the hydrazine hydrate solution of addition 9.38mL85% is into the buffer
Obtain the Tris-HCl-PEG- hydrazine hydrate buffer that concentration of hydrazine hydrate is 1.5mol/L.
From figure 3, it can be seen that clearly fingerprint lines can be shown on writing paper.
Embodiment 4
A kind of method that enzymatic reaction system shows for latent fingerprint, its step are as follows:
1, natural fingerprint sample preparation: the experimenter that please provide fingerprint cleans up hand, from doing, then puts on plastic glove,
It seals sweat and press...withes one's finger after ten minutes and be made on the brown paper of unstressed configuration ingredient, use that (simulation criminal investigation is handled a case after placing 4 hours
Actual conditions).
2, developing solution is prepared:
1) Tris-HCl- glycerol-hydrazine hydrate buffer (wherein concentration of hydrazine hydrate is 1.2mol/L): to 25mL0.2mol/
5mL0.1mol/LHCl solution is added in LTris solution, adds 20mL glycerol, adding water to 100mL to get pH is 9.0,0.05mol/
The Tris-HCl- glycerol buffer of L, then the hydrazine hydrate solution of 7.37mL85% is added up to concentration of hydrazine hydrate into the buffer
For Tris-HCl- glycerol-hydrazine hydrate buffer of 1.2mol/L.
2)0.009mol/LNAD+: by 0.060gNAD+It is added in 10mL distilled water to obtain the final product.
3) 80U/mL lactic dehydrogenase: 16mg lactic dehydrogenase is dissolved with 6mL distilled water to obtain the final product.
After each solution prepares, it is spare to be placed in refrigerator.
3, show: successively dipping Tris-HCl- glycerol-hydrazine hydrate buffer, 0.009mol/LNAD with fine, soft fur brush+With
To smearing at the fingerprint of step 1 sample preparation, smearing carries out 80U/mL lactic dehydrogenase at same direction, does not smear repeatedly and few
Amount after slightly drying, is excited with the ultraviolet light irradiation of 365nm wavelength, has fingerprint manifestation, photograph to record.
Compare clearly fingerprint lines from fig. 4, it can be seen that can show on brown paper.
Embodiment 5
A kind of method that enzymatic reaction system shows for latent fingerprint, its step are as follows:
1, natural fingerprint sample preparation: the experimenter that please provide fingerprint cleans up hand, from doing, then puts on plastic glove,
It seals sweat and press...withes one's finger after ten minutes and be made on the filter paper of unstressed configuration ingredient, (the reality handled a case of simulation criminal investigation is used after placing 4 hours
Border situation).
2, developing solution is prepared:
1) Tris-HCl-PEG- hydrazine hydrate buffer (wherein concentration of hydrazine hydrate is 1.8mol/L): to 25mL0.2mol/
In LTris solution be added 5mL0.1mol/LHCl solution, add 15mLPEG400, add water to 100mL to get pH be 9.0,
The Tris-HCl-PEG buffer of 0.05mol/L, then the hydrazine hydrate solution of 11.47mL85% is added up to water into the buffer
Close the Tris-HCl-PEG- hydrazine hydrate buffer that hydrazine concentration is 1.8mol/L.
2)0.009mol/LNAD+: by 0.060gNAD+It is added in 10mL distilled water to obtain the final product.
3) 50U/mL lactic dehydrogenase: 10mg lactic dehydrogenase is dissolved with 6mL distilled water to obtain the final product.
After each solution prepares, it is spare to be placed in refrigerator.
3, show: successively dipping Tris-HCl-PEG- hydrazine hydrate buffer, 0.009mol/LNAD with fine, soft fur brush+And 50U/
To smearing at the fingerprint of step 1 sample preparation, smearing carries out mL lactic dehydrogenase at same direction, does not smear repeatedly and a small amount of,
After slightly drying, is excited with the ultraviolet light irradiation of 365nm wavelength, have fingerprint manifestation, photograph to record.
Compare clearly fingerprint lines from fig. 5, it can be seen that can show on filter paper.
Claims (6)
1. a kind of enzymatic reaction system is used for the method that latent fingerprint shows, which is characterized in that steps are as follows:
(1) enzymatic reaction system developing solution is prepared, it is spare to be placed in refrigerator；
(2) enzymatic reaction system developing solution is sprayed or be coated onto may have fingerprint but without fluorescent material fingerprint object on；
(3) develop under ultraviolet lighting；
The enzymatic reaction system developing solution includes: 50-80U/mL lactic dehydrogenase, 0.007-0.009mol/L oxidized form nicotinoyl
Amine adenine-dinucleotide and Tris-HCl- hydrazine hydrate buffer；
Sequence enzymatic reaction system developing solution spray or be coated on object are as follows: first spray or apply Tris-HCl- hydrazine hydrate buffering
Liquid and oxidized nicotinamide adenine dinucleotide finally spray or apply lactic dehydrogenase, wherein Tris-HCl- hydrazine hydrate buffer
It is unlimited with oxidized nicotinamide adenine dinucleotide sequencing, or spray or apply simultaneously；
The preparation method of the Tris-HCl- hydrazine hydrate buffer: first configuring pH9.0,0.05mol/L Tris-HCl buffer,
Hydrazine hydrate is added thereto again makes the final concentration of 1.2 ~ 1.8mol/L of hydrazine hydrate.
2. the method according to claim 1, wherein in the pH9.0,0.05mol/L Tris-HCl buffer
Contain the glycerol and/or polyethylene glycol 400 accounted within the pH9.0,0.05mol/L Tris-HCl buffer volume 40%.
3. the method according to claim 1, wherein in the pH9.0,0.05mol/L Tris-HCl buffer
Contain the glycerol and/or polyethylene glycol 400 accounted within the pH9.0,0.05mol/L Tris-HCl buffer volume 25%.
4. method according to claim 1 to 3, it is characterised in that: the oxidized form two nucleoside of nicotinamide adenine
Acid is β-nicotinamide adenine dinucleotide.
5. method according to claim 1 to 3, it is characterised in that: the fingerprint is invisible fingerprints of sweat.
6. method according to claim 1 to 3, it is characterised in that: the fingerprint object is the book of unstressed configuration ingredient
Write one of paper, brown paper, filter paper, art paper or newspaper.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103919558A (en) * | 2014-04-13 | 2014-07-16 | 中南民族大学 | Method of adopting fluorescence quenching system to potential fingerprint display |
| CN104783806A (en) * | 2015-05-05 | 2015-07-22 | 哈尔滨师范大学 | Method for displaying latent fingerprints through fluorescent copper sol |
| CN106645062A (en) * | 2016-11-30 | 2017-05-10 | 青岛农业大学 | Method for laten fingerprint fluorescence development by applying aptamer and lysozyme for specific recognition and formation of G-quadruplex |
| US20180039813A1 (en) * | 2016-08-04 | 2018-02-08 | Howard A. Harris | Latent fingerprint development on porous surfaces |
-
2019
- 2019-08-06 CN CN201910722660.3A patent/CN110464361B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103919558A (en) * | 2014-04-13 | 2014-07-16 | 中南民族大学 | Method of adopting fluorescence quenching system to potential fingerprint display |
| CN104783806A (en) * | 2015-05-05 | 2015-07-22 | 哈尔滨师范大学 | Method for displaying latent fingerprints through fluorescent copper sol |
| US20180039813A1 (en) * | 2016-08-04 | 2018-02-08 | Howard A. Harris | Latent fingerprint development on porous surfaces |
| CN106645062A (en) * | 2016-11-30 | 2017-05-10 | 青岛农业大学 | Method for laten fingerprint fluorescence development by applying aptamer and lysozyme for specific recognition and formation of G-quadruplex |
Non-Patent Citations (2)
| Title |
|---|
| 天津市滨海新区汉沽人民检察院编: "《检查实务若干问题研究》", 31 December 2018 * |
| 庹浔: "乳酸含量的酶法测定", 《中国优秀博硕士学位论文全文数据库（硕士）工程科技Ⅰ辑》 * |
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