CN104777274B - Fixing hold-down device for PDMS micro flow cell - Google Patents
Fixing hold-down device for PDMS micro flow cell Download PDFInfo
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- CN104777274B CN104777274B CN201510188488.XA CN201510188488A CN104777274B CN 104777274 B CN104777274 B CN 104777274B CN 201510188488 A CN201510188488 A CN 201510188488A CN 104777274 B CN104777274 B CN 104777274B
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- base
- flow cell
- pdms
- micrometer head
- fixed
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- 239000004205 dimethyl polysiloxane Substances 0.000 title claims abstract description 30
- 235000013870 dimethyl polysiloxane Nutrition 0.000 title claims abstract description 30
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 title claims abstract description 30
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 title claims abstract description 29
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 title claims abstract description 29
- 238000003825 pressing Methods 0.000 claims description 16
- 210000004907 gland Anatomy 0.000 claims description 11
- 230000000694 effects Effects 0.000 claims description 5
- 238000010276 construction Methods 0.000 claims description 4
- -1 workbench Chemical compound 0.000 claims description 3
- 230000006835 compression Effects 0.000 abstract description 11
- 238000007906 compression Methods 0.000 abstract description 11
- 230000001105 regulatory effect Effects 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 description 27
- 239000012530 fluid Substances 0.000 description 5
- 238000009434 installation Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 230000005672 electromagnetic field Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000007789 sealing Methods 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 238000013475 authorization Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005056 compaction Methods 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 238000005034 decoration Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000005357 flat glass Substances 0.000 description 1
- UQEAIHBTYFGYIE-UHFFFAOYSA-N hexamethyldisiloxane Polymers C[Si](C)(C)O[Si](C)(C)C UQEAIHBTYFGYIE-UHFFFAOYSA-N 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000013139 quantization Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 239000004945 silicone rubber Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a fixing hold-down device for a PDMS micro flow cell. The fixing hold-down device comprises a worktable, a base mounted on the worktable and used for bearing the PDMS micro flow cell, and a support frame mounted on the worktable and crossing the base; a spiral micrometer head is vertically mounted at the top of the support frame; the micrometric screw of the spiral micrometer head serves as a compression bar, and the tail end of the compression bar penetrates through the top of the support frame and faces the base. According to the fixing hold-down device for the PDMS micro flow cell, as the spiral micrometer head serves as a device for regulating hold-down force of the PDMS micro flow cell and the base, the pressure can be controlled by use of scale marks on the spiral micrometer head when the flow cell is fixed, and the appropriate position can be adjusted quickly; as a result, the times of operation are reduced, the operation efficiency is improved, and meanwhile, the damage to the flow cell due to multiple adjustment is avoided.
Description
Technical field
The present invention relates to a kind of hold down gag, more particularly to a kind of device that is fixed and clamped for the miniature flow cells of PDMS.
Background technology
Polydimethylsiloxane (Polydimethylsiloxane, PDMS) is commonly called as silicone rubber, by its low cost, well
Chemical inertness, same to silicon chip, the good adhesion between sheet metal, sheet glass, and various modified repair can be carried out on surface
The advantages of decorations, in recent years, it is widely used in the field such as micro-fluidic.
As shown in figure 3, the miniature flow cells 6 of PDMS include:Base plate 61 and the PDMS modules 62 being fixed on base plate, in figure,
Upward, when actually used, generally towards lower installation, base plate 61 is provided with inlet 63, liquid outlet 64 and location hole to PDMS modules 62
65。
During the use of the miniature flow cells of PDMS, need that it is fitted and is given certain pressure with installation surface
Clamp force.Whether the thrust is suitable extremely important, if too small can cause flow cell the phenomenon of leakage occur, and it is excessive, can make molten
Liquid circulation is not smooth, and flow cell may be caused to damage.In the past the compression mode of flow cell was typically adopted and placed one above it
Block weight, then again in a screw tightening installed above.
The patent documentation of Authorization Notice No. CN 100549683C discloses a kind of multifunctional micro-electrode chip circulation detecting pool
Device, lower platen upper surface is embedded with electromagnetic field coil, and two sides are respectively equipped with guide rail electrode, guide rail electrode respectively with electromagnetic field
The coil lead electrical connection of coil;Elastica lower surface is embedded with micro-electrode chip, and upper surface is provided with circulation groove;Top board two
Side is respectively equipped with guide rail electrode, and guide rail electrode is electrically connected respectively with the contact conductor of micro-electrode chip, and top board is provided with stream
Body entrance and fluid issuing;Top board, lower platen and elastica are fixed by fixing bolt, and two pipe jointers are respectively pipeline
Installed in the fluid intake (inlet) and fluid issuing (liquid outlet) of top board;Wherein micro-electrode chip is located under elastica
Top that is square and being located at lower platen, electromagnetic field coil is located at the lower section of micro-electrode chip and is located at the top of lower platen, and fluid enters
Mouth and fluid issuing are respectively aligned to the two-port in elastica circulation groove, and the inventive structure is simple, and function is advanced, install and clean
It is convenient, the fields such as biology, chemistry, life sciences can be used in and widely used.
Adopt and there is certain defect in this way, one be the adjustment every time to screw (fixing bolt) be all by checking
Going out liquid situation and flow cell whether leakage just knowing whether for liquid outlet has been screwed to suitable position and thrust now
If appropriate for mainly being judged by rule of thumb by operator, it is impossible to accomplish the control of digital quantization, and regulated efficiency is low, effect
Difference;Two is the meaningless adjustment of screw position when using, and the miniature flow cells of PDMS can be caused damage in itself, so as to shorten flow cell
Service life, improve experimental cost.Therefore, suitable hold down gag is designed, for micro-fluidic chip and its application are weighed very much
Will.
The content of the invention
The invention provides a kind of device that is fixed and clamped for the miniature flow cells of PDMS, simple structure, it is good to compress effect,
It is easy to adjust, quick.
A kind of device that is fixed and clamped for the miniature flow cells of PDMS, including workbench, and install use on the table
In the base for carrying the miniature flow cells of PDMS, also include being arranged on the workbench and across the stand frame of base, the support
The top vertical of frame is provided with spiral micrometer head, and used as depression bar, its end passes through support to the micrometric screw of the spiral micrometer head
The top of frame is towards base.
When the present invention is used, first the miniature flow cells of PDMS (hereinafter referred to as flow cell) are placed on base, spiral micrometer head
Lower section, after being in place, then by adjustable screw micrometer head, make micrometric screw compress flow cell, after installing, start
During test, buffer is passed through to flow cell to check compression effect, that is, observes flow cell whether leakage or tension, if compressing first
Power is too small, then flow out from liquid outlet without liquid, and is able to observe that leakage around flow cell, needs adjustable screw micrometer head,
Increase and observed again after pressure, until liquid flows out from liquid outlet, and then compression degree is illustrated just without leakage around flow cell,
Write down the numerical value of now spiral micrometer head;If thrust is excessive, deform can the microchannel of flow cell and to go out liquid not smooth, this
When liquid outlet liquid outlet quantity be significantly smaller than inlet liquid inlet volume, need adjustable screw micrometer head, reduce and observed again after pressure, go out liquid
When the liquid outlet quantity of mouth is consistent substantially with inlet liquid inlet volume, then compression degree is illustrated just, write down now spiral micrometer head
Numerical value.
According to above-mentioned control method using the present invention, through adjustable screw micrometer head several times, finding out can make flow cell normal
The maxima and minima of the spiral micrometer head of work, when reusing this flow cell later, only need to adjust according to this numerical range
Section spiral micrometer head, so as to improve regulation efficiency, reduces number of operations, efficiently avoid in prior art repeatedly
Adjustment bolt causes to damage to flow cell.
The base is used to carry flow cell, and the operating position of flow cell is adjusted for convenience, it is preferred that the base is lived
Dynamic to install, the workbench is provided with the locking piece of locking base.
For the ease of installing and manufacturing, it is preferred that the workbench is provided with the chute of the base that is slidably installed, described
Locking piece is the clamping screw on chute.
For the ease of the installation and removal of flow cell, it is preferred that the stand frame includes:
Two groups of vertical rods, fix on the table, respectively positioned at the both sides of base;
Gland, side is hinged on the top of one group of vertical rod, and opposite side is connected with another group of vertical rod by movable securing member, institute
The construction section for stating spiral micrometer head is fixed on the middle part of gland.
Said structure makes gland to open, and is easy to the installation and removal of flow cell.
It is further preferred that the movable securing member is movable bolt.
In order that the microchannel surface uniform force of flow cell, so that flow cell is installed in place, sealing effectiveness is preferable,
Preferably, the pressing plate of uniform pressure is provided with below the micrometric screw.
For the ease of positioning flow cell, it is preferred that be fixed with least two locating rods on the base.Flow cell is provided with
The through hole coordinated with locating rod.
It is further preferred that the pressing plate is provided with the location hole coordinated with each locating rod.
In order that flow cell uniform force, pressing plate realizes the para-position with flow cell by locating rod cooperation, it is possible to achieve
The centre position of flow cell working surface is covered in, now only micrometric screw is directed at the center of pressing plate, just can guarantee that flow cell
Uniform force, it is preferred that the workbench is provided with two tracks being arranged symmetrically, it is right that the bottom of each group vertical rod is slidably mounted on
In the track answered;
The gland is provided with the slideway of the vertical track, and the construction section of the spiral micrometer head is slidably mounted on the cunning
In road.
Preferably, the end face center of the pressing plate is provided with the registration holes of taper, and the end of the micrometric screw is and this pair
The taper that position hole coordinates.
By mobile stand frame and spiral micrometer head come the center of adjustable screw micrometer head to pressing plate, and by cone
The registration holes of shape coordinate with micrometric screw, improve the accuracy of para-position, it is ensured that flow cell working surface uniform stressed.
Beneficial effects of the present invention:
The device that is fixed and clamped for the miniature flow cells of PDMS of the present invention, due to micro- as PDMS using spiral micrometer head
The adjusting means of type flow cell and base thrust, thus in fixed flow cell can by the scale in spiral micrometer head come
The size of control pressure, is quickly adjusted to suitable position, so as to reduce number of operations, operating efficiency is improved, while avoiding
Repeatedly adjustment is to the infringement caused by flow cell.
Description of the drawings
Fig. 1 is the dimensional structure diagram of the present embodiment 1.
Fig. 2 is dimensional structure diagram when the present embodiment 1 is used.
Fig. 3 is the dimensional structure diagram of the miniature flow cells of PDMS.
Fig. 4 is the structural representation of the spiral micrometer head of the present embodiment 1.
Fig. 5 is the dimensional structure diagram of the present embodiment 2.
Specific embodiment
Embodiment 1
As shown in Fig. 1,2 and 4, the device that is fixed and clamped for the miniature flow cells of PDMS of the present embodiment includes:Workbench
1, it is used to carry the base 2 of the miniature flow cells of PDMS on workbench 1, on the workbench and across base 2
Stand frame 3, the top vertical of stand frame 3 is provided with spiral micrometer head 4, the micrometric screw 41 of spiral micrometer head 4 as depression bar, its
End passes through the top of stand frame 3 towards base 2.
Workbench 1 is provided with the chute 11 of the base 2 that is slidably installed, and the clamping screw of locking base 2 is provided with chute 11
12。
Stand frame 3 includes:Two groups of vertical rods 31, are fixed on workbench 1, respectively positioned at the both sides of base 2;Gland 32, one
Side is hinged on the top of one group of vertical rod, and opposite side is connected with another group of vertical rod by movable bolt 33, the installation of spiral micrometer head 4
Section 42 is fixed on the middle part of gland 32.
The present embodiment also includes that the lower section for being located at micrometric screw 41 is used for the pressing plate 5 of uniform pressure, the both sides difference of base 2
It is fixed with locating rod 21.
Pressing plate 5 is provided with two location holes 51 coordinated with each locating rod 21.
When the present embodiment is used, gland 32 is opened, as needed the position of adjusting base 2, after adjusting in place, clamping screw
12 tighten, and lock base 2, and then the miniature flow cells 6 of PDMS (hereinafter referred to as flow cell 6) is installed on base, by positioning
Bar 21 coordinates to position flow cell 6 with location hole 65, then installs pressing plate 5, coordinates to determine with location hole 51 by locating rod 21
Position pressing plate 5, is then shut off gland 32, and is fixed by movable bolt 33, and then the clamping platen 5 of adjustable screw micrometer head 4, passes through
The pressure that pressing plate 5 carrys out the applying of uniform spiral micrometer head 4 is set, pressure is evenly distributed on flow cell 6, make flow cell 6
The homogeneous deformation of PDMS modules 62, improve sealing.
After completing preliminary compression, tested, be passed through buffer to check compression effect to flow cell 6 first, that is, observed
Whether flow cell leakage or tension, if thrust is too small, flow out without liquid from liquid outlet 64, and is able to observe that flow cell
6 surrounding leakages, need adjustable screw micrometer head 4, increase and are observed again after pressure, until liquid flows out from liquid outlet 64, and circulate
Without leakage around pond, then compression degree is illustrated just, write down the numerical value of now spiral micrometer head;If thrust is excessive, can make
The microchannel deformation of flow cell 6 and go out that liquid is not smooth, now the liquid outlet quantity of liquid outlet is significantly smaller than inlet liquid inlet volume, needs to adjust
Section spiral micrometer head 4, reduces and is observed again after pressure, when liquid outlet quantity and the inlet liquid inlet volume of liquid outlet 64 are consistent substantially,
Then illustrate compression degree just, write down the numerical value of now spiral micrometer head 4.
The present embodiment is used according to above-mentioned control method, through adjustable screw micrometer head 4 several times, finding out can make flow cell just
The maxima and minima of the spiral micrometer head 4 for often working, when reusing this flow cell 6 later, only need to be according to this numerical range
Carry out adjustable screw micrometer head, so as to improve regulation efficiency, number of operations is reduced, in efficiently avoid prior art
Repeatedly adjustment bolt causes to damage to flow cell.
Embodiment 2
As shown in figure 5, except stand frame and the mounting means of spiral micrometer head, also pressing plate and micrometer spiral shell in the present embodiment
Beyond the fit system of bar is different from embodiment 1, remaining structure is same as Example 1.
Workbench 1 is provided with two tracks 7 being arranged symmetrically, and the bottom of each group vertical rod 31 is slidably mounted on corresponding track
In 7;
Gland 32 is provided with the slideway 8 of vertical track 7, and the construction section 42 of spiral micrometer head 4 is slidably mounted on the slideway 8
It is interior.
The end face center of pressing plate 5 is provided with the registration holes 52 of taper, and the end of micrometric screw 41 is to coordinate with registration holes 52
Taper.
Before compression, by mobile stand frame 3 and spiral micrometer head 4 come the center of adjustable screw micrometer head 4 to pressing plate
Position, in compaction process, coordinates further through the registration holes 52 and micrometric screw 41 of taper, is adjusted to adjustable screw micrometer head
Position, improves the accuracy of para-position, it is ensured that flow cell working surface uniform stressed.
Claims (5)
1. a kind of device that is fixed and clamped for the miniature flow cells of PDMS, including workbench, and install be used on the table
Carry the base of the miniature flow cells of PDMS, it is characterised in that also include being arranged on the workbench and across the stand frame of base,
The top vertical of the stand frame is provided with spiral micrometer head, the micrometric screw of the spiral micrometer head as depression bar, its end
Top through stand frame is towards base;
The pressing plate of uniform pressure is provided with below the micrometric screw;
At least two locating rods are fixed with the base;
The pressing plate is provided with the location hole coordinated with each locating rod.
2. the device that is fixed and clamped of the miniature flow cells of PDMS is used for as claimed in claim 1, it is characterised in that the base is lived
Dynamic to install, the workbench is provided with the locking piece of locking base.
3. the device that is fixed and clamped of the miniature flow cells of PDMS is used for as claimed in claim 2, it is characterised in that the workbench
The chute of the base that is slidably installed is provided with, the locking piece is the clamping screw on chute.
4. the device that is fixed and clamped of the miniature flow cells of PDMS is used for as claimed in claim 1, it is characterised in that the stand frame
Including:
Two groups of vertical rods, fix on the table, respectively positioned at the both sides of base;
Gland, side is hinged on the top of one group of vertical rod, and opposite side is connected with another group of vertical rod by movable securing member, the spiral shell
The construction section of rotation micrometer head is fixed on the middle part of gland.
5. the device that is fixed and clamped of the miniature flow cells of PDMS is used for as claimed in claim 4, it is characterised in that the activity is tight
Firmware is movable bolt.
Priority Applications (1)
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CN201510188488.XA CN104777274B (en) | 2015-04-20 | 2015-04-20 | Fixing hold-down device for PDMS micro flow cell |
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CN201510188488.XA CN104777274B (en) | 2015-04-20 | 2015-04-20 | Fixing hold-down device for PDMS micro flow cell |
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CN104777274A CN104777274A (en) | 2015-07-15 |
CN104777274B true CN104777274B (en) | 2017-04-19 |
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CN1891646A (en) * | 2005-07-08 | 2007-01-10 | 中国科学院大连化学物理研究所 | Normal temperature bonding method for quartz microflow control chip and pressurizing mould |
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