CN104777274B - Fixing hold-down device for PDMS micro flow cell - Google Patents

Fixing hold-down device for PDMS micro flow cell Download PDF

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Publication number
CN104777274B
CN104777274B CN201510188488.XA CN201510188488A CN104777274B CN 104777274 B CN104777274 B CN 104777274B CN 201510188488 A CN201510188488 A CN 201510188488A CN 104777274 B CN104777274 B CN 104777274B
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base
flow cell
pdms
micrometer head
fixed
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CN104777274A (en
Inventor
王晓萍
庞凯
张冰
董伟
洪夏云
施春飞
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Zhejiang Nuojia Biological Technology Co Ltd
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Zhejiang University ZJU
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Abstract

The invention discloses a fixing hold-down device for a PDMS micro flow cell. The fixing hold-down device comprises a worktable, a base mounted on the worktable and used for bearing the PDMS micro flow cell, and a support frame mounted on the worktable and crossing the base; a spiral micrometer head is vertically mounted at the top of the support frame; the micrometric screw of the spiral micrometer head serves as a compression bar, and the tail end of the compression bar penetrates through the top of the support frame and faces the base. According to the fixing hold-down device for the PDMS micro flow cell, as the spiral micrometer head serves as a device for regulating hold-down force of the PDMS micro flow cell and the base, the pressure can be controlled by use of scale marks on the spiral micrometer head when the flow cell is fixed, and the appropriate position can be adjusted quickly; as a result, the times of operation are reduced, the operation efficiency is improved, and meanwhile, the damage to the flow cell due to multiple adjustment is avoided.

Description

A kind of device that is fixed and clamped for the miniature flow cells of PDMS
Technical field
The present invention relates to a kind of hold down gag, more particularly to a kind of device that is fixed and clamped for the miniature flow cells of PDMS.
Background technology
Polydimethylsiloxane (Polydimethylsiloxane, PDMS) is commonly called as silicone rubber, by its low cost, well Chemical inertness, same to silicon chip, the good adhesion between sheet metal, sheet glass, and various modified repair can be carried out on surface The advantages of decorations, in recent years, it is widely used in the field such as micro-fluidic.
As shown in figure 3, the miniature flow cells 6 of PDMS include:Base plate 61 and the PDMS modules 62 being fixed on base plate, in figure, Upward, when actually used, generally towards lower installation, base plate 61 is provided with inlet 63, liquid outlet 64 and location hole to PDMS modules 62 65。
During the use of the miniature flow cells of PDMS, need that it is fitted and is given certain pressure with installation surface Clamp force.Whether the thrust is suitable extremely important, if too small can cause flow cell the phenomenon of leakage occur, and it is excessive, can make molten Liquid circulation is not smooth, and flow cell may be caused to damage.In the past the compression mode of flow cell was typically adopted and placed one above it Block weight, then again in a screw tightening installed above.
The patent documentation of Authorization Notice No. CN 100549683C discloses a kind of multifunctional micro-electrode chip circulation detecting pool Device, lower platen upper surface is embedded with electromagnetic field coil, and two sides are respectively equipped with guide rail electrode, guide rail electrode respectively with electromagnetic field The coil lead electrical connection of coil;Elastica lower surface is embedded with micro-electrode chip, and upper surface is provided with circulation groove;Top board two Side is respectively equipped with guide rail electrode, and guide rail electrode is electrically connected respectively with the contact conductor of micro-electrode chip, and top board is provided with stream Body entrance and fluid issuing;Top board, lower platen and elastica are fixed by fixing bolt, and two pipe jointers are respectively pipeline Installed in the fluid intake (inlet) and fluid issuing (liquid outlet) of top board;Wherein micro-electrode chip is located under elastica Top that is square and being located at lower platen, electromagnetic field coil is located at the lower section of micro-electrode chip and is located at the top of lower platen, and fluid enters Mouth and fluid issuing are respectively aligned to the two-port in elastica circulation groove, and the inventive structure is simple, and function is advanced, install and clean It is convenient, the fields such as biology, chemistry, life sciences can be used in and widely used.
Adopt and there is certain defect in this way, one be the adjustment every time to screw (fixing bolt) be all by checking Going out liquid situation and flow cell whether leakage just knowing whether for liquid outlet has been screwed to suitable position and thrust now If appropriate for mainly being judged by rule of thumb by operator, it is impossible to accomplish the control of digital quantization, and regulated efficiency is low, effect Difference;Two is the meaningless adjustment of screw position when using, and the miniature flow cells of PDMS can be caused damage in itself, so as to shorten flow cell Service life, improve experimental cost.Therefore, suitable hold down gag is designed, for micro-fluidic chip and its application are weighed very much Will.
The content of the invention
The invention provides a kind of device that is fixed and clamped for the miniature flow cells of PDMS, simple structure, it is good to compress effect, It is easy to adjust, quick.
A kind of device that is fixed and clamped for the miniature flow cells of PDMS, including workbench, and install use on the table In the base for carrying the miniature flow cells of PDMS, also include being arranged on the workbench and across the stand frame of base, the support The top vertical of frame is provided with spiral micrometer head, and used as depression bar, its end passes through support to the micrometric screw of the spiral micrometer head The top of frame is towards base.
When the present invention is used, first the miniature flow cells of PDMS (hereinafter referred to as flow cell) are placed on base, spiral micrometer head Lower section, after being in place, then by adjustable screw micrometer head, make micrometric screw compress flow cell, after installing, start During test, buffer is passed through to flow cell to check compression effect, that is, observes flow cell whether leakage or tension, if compressing first Power is too small, then flow out from liquid outlet without liquid, and is able to observe that leakage around flow cell, needs adjustable screw micrometer head, Increase and observed again after pressure, until liquid flows out from liquid outlet, and then compression degree is illustrated just without leakage around flow cell, Write down the numerical value of now spiral micrometer head;If thrust is excessive, deform can the microchannel of flow cell and to go out liquid not smooth, this When liquid outlet liquid outlet quantity be significantly smaller than inlet liquid inlet volume, need adjustable screw micrometer head, reduce and observed again after pressure, go out liquid When the liquid outlet quantity of mouth is consistent substantially with inlet liquid inlet volume, then compression degree is illustrated just, write down now spiral micrometer head Numerical value.
According to above-mentioned control method using the present invention, through adjustable screw micrometer head several times, finding out can make flow cell normal The maxima and minima of the spiral micrometer head of work, when reusing this flow cell later, only need to adjust according to this numerical range Section spiral micrometer head, so as to improve regulation efficiency, reduces number of operations, efficiently avoid in prior art repeatedly Adjustment bolt causes to damage to flow cell.
The base is used to carry flow cell, and the operating position of flow cell is adjusted for convenience, it is preferred that the base is lived Dynamic to install, the workbench is provided with the locking piece of locking base.
For the ease of installing and manufacturing, it is preferred that the workbench is provided with the chute of the base that is slidably installed, described Locking piece is the clamping screw on chute.
For the ease of the installation and removal of flow cell, it is preferred that the stand frame includes:
Two groups of vertical rods, fix on the table, respectively positioned at the both sides of base;
Gland, side is hinged on the top of one group of vertical rod, and opposite side is connected with another group of vertical rod by movable securing member, institute The construction section for stating spiral micrometer head is fixed on the middle part of gland.
Said structure makes gland to open, and is easy to the installation and removal of flow cell.
It is further preferred that the movable securing member is movable bolt.
In order that the microchannel surface uniform force of flow cell, so that flow cell is installed in place, sealing effectiveness is preferable, Preferably, the pressing plate of uniform pressure is provided with below the micrometric screw.
For the ease of positioning flow cell, it is preferred that be fixed with least two locating rods on the base.Flow cell is provided with The through hole coordinated with locating rod.
It is further preferred that the pressing plate is provided with the location hole coordinated with each locating rod.
In order that flow cell uniform force, pressing plate realizes the para-position with flow cell by locating rod cooperation, it is possible to achieve The centre position of flow cell working surface is covered in, now only micrometric screw is directed at the center of pressing plate, just can guarantee that flow cell Uniform force, it is preferred that the workbench is provided with two tracks being arranged symmetrically, it is right that the bottom of each group vertical rod is slidably mounted on In the track answered;
The gland is provided with the slideway of the vertical track, and the construction section of the spiral micrometer head is slidably mounted on the cunning In road.
Preferably, the end face center of the pressing plate is provided with the registration holes of taper, and the end of the micrometric screw is and this pair The taper that position hole coordinates.
By mobile stand frame and spiral micrometer head come the center of adjustable screw micrometer head to pressing plate, and by cone The registration holes of shape coordinate with micrometric screw, improve the accuracy of para-position, it is ensured that flow cell working surface uniform stressed.
Beneficial effects of the present invention:
The device that is fixed and clamped for the miniature flow cells of PDMS of the present invention, due to micro- as PDMS using spiral micrometer head The adjusting means of type flow cell and base thrust, thus in fixed flow cell can by the scale in spiral micrometer head come The size of control pressure, is quickly adjusted to suitable position, so as to reduce number of operations, operating efficiency is improved, while avoiding Repeatedly adjustment is to the infringement caused by flow cell.
Description of the drawings
Fig. 1 is the dimensional structure diagram of the present embodiment 1.
Fig. 2 is dimensional structure diagram when the present embodiment 1 is used.
Fig. 3 is the dimensional structure diagram of the miniature flow cells of PDMS.
Fig. 4 is the structural representation of the spiral micrometer head of the present embodiment 1.
Fig. 5 is the dimensional structure diagram of the present embodiment 2.
Specific embodiment
Embodiment 1
As shown in Fig. 1,2 and 4, the device that is fixed and clamped for the miniature flow cells of PDMS of the present embodiment includes:Workbench 1, it is used to carry the base 2 of the miniature flow cells of PDMS on workbench 1, on the workbench and across base 2 Stand frame 3, the top vertical of stand frame 3 is provided with spiral micrometer head 4, the micrometric screw 41 of spiral micrometer head 4 as depression bar, its End passes through the top of stand frame 3 towards base 2.
Workbench 1 is provided with the chute 11 of the base 2 that is slidably installed, and the clamping screw of locking base 2 is provided with chute 11 12。
Stand frame 3 includes:Two groups of vertical rods 31, are fixed on workbench 1, respectively positioned at the both sides of base 2;Gland 32, one Side is hinged on the top of one group of vertical rod, and opposite side is connected with another group of vertical rod by movable bolt 33, the installation of spiral micrometer head 4 Section 42 is fixed on the middle part of gland 32.
The present embodiment also includes that the lower section for being located at micrometric screw 41 is used for the pressing plate 5 of uniform pressure, the both sides difference of base 2 It is fixed with locating rod 21.
Pressing plate 5 is provided with two location holes 51 coordinated with each locating rod 21.
When the present embodiment is used, gland 32 is opened, as needed the position of adjusting base 2, after adjusting in place, clamping screw 12 tighten, and lock base 2, and then the miniature flow cells 6 of PDMS (hereinafter referred to as flow cell 6) is installed on base, by positioning Bar 21 coordinates to position flow cell 6 with location hole 65, then installs pressing plate 5, coordinates to determine with location hole 51 by locating rod 21 Position pressing plate 5, is then shut off gland 32, and is fixed by movable bolt 33, and then the clamping platen 5 of adjustable screw micrometer head 4, passes through The pressure that pressing plate 5 carrys out the applying of uniform spiral micrometer head 4 is set, pressure is evenly distributed on flow cell 6, make flow cell 6 The homogeneous deformation of PDMS modules 62, improve sealing.
After completing preliminary compression, tested, be passed through buffer to check compression effect to flow cell 6 first, that is, observed Whether flow cell leakage or tension, if thrust is too small, flow out without liquid from liquid outlet 64, and is able to observe that flow cell 6 surrounding leakages, need adjustable screw micrometer head 4, increase and are observed again after pressure, until liquid flows out from liquid outlet 64, and circulate Without leakage around pond, then compression degree is illustrated just, write down the numerical value of now spiral micrometer head;If thrust is excessive, can make The microchannel deformation of flow cell 6 and go out that liquid is not smooth, now the liquid outlet quantity of liquid outlet is significantly smaller than inlet liquid inlet volume, needs to adjust Section spiral micrometer head 4, reduces and is observed again after pressure, when liquid outlet quantity and the inlet liquid inlet volume of liquid outlet 64 are consistent substantially, Then illustrate compression degree just, write down the numerical value of now spiral micrometer head 4.
The present embodiment is used according to above-mentioned control method, through adjustable screw micrometer head 4 several times, finding out can make flow cell just The maxima and minima of the spiral micrometer head 4 for often working, when reusing this flow cell 6 later, only need to be according to this numerical range Carry out adjustable screw micrometer head, so as to improve regulation efficiency, number of operations is reduced, in efficiently avoid prior art Repeatedly adjustment bolt causes to damage to flow cell.
Embodiment 2
As shown in figure 5, except stand frame and the mounting means of spiral micrometer head, also pressing plate and micrometer spiral shell in the present embodiment Beyond the fit system of bar is different from embodiment 1, remaining structure is same as Example 1.
Workbench 1 is provided with two tracks 7 being arranged symmetrically, and the bottom of each group vertical rod 31 is slidably mounted on corresponding track In 7;
Gland 32 is provided with the slideway 8 of vertical track 7, and the construction section 42 of spiral micrometer head 4 is slidably mounted on the slideway 8 It is interior.
The end face center of pressing plate 5 is provided with the registration holes 52 of taper, and the end of micrometric screw 41 is to coordinate with registration holes 52 Taper.
Before compression, by mobile stand frame 3 and spiral micrometer head 4 come the center of adjustable screw micrometer head 4 to pressing plate Position, in compaction process, coordinates further through the registration holes 52 and micrometric screw 41 of taper, is adjusted to adjustable screw micrometer head Position, improves the accuracy of para-position, it is ensured that flow cell working surface uniform stressed.

Claims (5)

1. a kind of device that is fixed and clamped for the miniature flow cells of PDMS, including workbench, and install be used on the table Carry the base of the miniature flow cells of PDMS, it is characterised in that also include being arranged on the workbench and across the stand frame of base, The top vertical of the stand frame is provided with spiral micrometer head, the micrometric screw of the spiral micrometer head as depression bar, its end Top through stand frame is towards base;
The pressing plate of uniform pressure is provided with below the micrometric screw;
At least two locating rods are fixed with the base;
The pressing plate is provided with the location hole coordinated with each locating rod.
2. the device that is fixed and clamped of the miniature flow cells of PDMS is used for as claimed in claim 1, it is characterised in that the base is lived Dynamic to install, the workbench is provided with the locking piece of locking base.
3. the device that is fixed and clamped of the miniature flow cells of PDMS is used for as claimed in claim 2, it is characterised in that the workbench The chute of the base that is slidably installed is provided with, the locking piece is the clamping screw on chute.
4. the device that is fixed and clamped of the miniature flow cells of PDMS is used for as claimed in claim 1, it is characterised in that the stand frame Including:
Two groups of vertical rods, fix on the table, respectively positioned at the both sides of base;
Gland, side is hinged on the top of one group of vertical rod, and opposite side is connected with another group of vertical rod by movable securing member, the spiral shell The construction section of rotation micrometer head is fixed on the middle part of gland.
5. the device that is fixed and clamped of the miniature flow cells of PDMS is used for as claimed in claim 4, it is characterised in that the activity is tight Firmware is movable bolt.
CN201510188488.XA 2015-04-20 2015-04-20 Fixing hold-down device for PDMS micro flow cell Active CN104777274B (en)

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CN104777274B true CN104777274B (en) 2017-04-19

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1891646A (en) * 2005-07-08 2007-01-10 中国科学院大连化学物理研究所 Normal temperature bonding method for quartz microflow control chip and pressurizing mould
CN101079492A (en) * 2007-06-22 2007-11-28 中国科学院上海微系统与信息技术研究所 An encapsulation method for small fuel cell
CN101303327A (en) * 2008-06-20 2008-11-12 浙江大学 Microflow control chip clamping device
CN101966473A (en) * 2010-10-26 2011-02-09 武汉大学 Micro fluid control screening chip based on ultrasonic standing wave and preparation method thereof
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Effective date of registration: 20181108

Address after: 311200 Hangzhou, Zhejiang, Hangzhou, Xiaoshan economic and Technological Development Zone 198 Hangzhou Bay Information Port E 16 block.

Patentee after: Zhejiang Nuojia Biological Technology Co. Ltd.

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Patentee before: Zhejiang University

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