CN104774948A - PCR (Polymerase chain reaction) identification kit and identification method for largehead atractylode in Hebei - Google Patents
PCR (Polymerase chain reaction) identification kit and identification method for largehead atractylode in Hebei Download PDFInfo
- Publication number
- CN104774948A CN104774948A CN201510181371.9A CN201510181371A CN104774948A CN 104774948 A CN104774948 A CN 104774948A CN 201510181371 A CN201510181371 A CN 201510181371A CN 104774948 A CN104774948 A CN 104774948A
- Authority
- CN
- China
- Prior art keywords
- hebei
- pcr
- bighead atractylodes
- atractylodes rhizome
- hbz2
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 241000132012 Atractylodes Species 0.000 title claims abstract description 95
- 238000000034 method Methods 0.000 title claims abstract description 21
- 238000003752 polymerase chain reaction Methods 0.000 title abstract 4
- 102100030387 Hemoglobin subunit zeta Human genes 0.000 claims abstract description 35
- 101150078996 HBZ gene Proteins 0.000 claims abstract description 18
- 101000843058 Homo sapiens Hemoglobin subunit zeta Proteins 0.000 claims abstract description 17
- 238000001514 detection method Methods 0.000 claims description 29
- 238000012408 PCR amplification Methods 0.000 claims description 12
- 238000012797 qualification Methods 0.000 claims description 12
- 230000003321 amplification Effects 0.000 claims description 8
- 238000001962 electrophoresis Methods 0.000 claims description 6
- 238000000605 extraction Methods 0.000 claims description 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 5
- 238000000246 agarose gel electrophoresis Methods 0.000 claims description 3
- 230000004087 circulation Effects 0.000 claims description 3
- 238000004925 denaturation Methods 0.000 claims description 3
- 230000036425 denaturation Effects 0.000 claims description 3
- 239000000284 extract Substances 0.000 claims description 3
- 239000012160 loading buffer Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000004153 renaturation Methods 0.000 claims description 3
- 238000012360 testing method Methods 0.000 claims description 3
- 239000003814 drug Substances 0.000 abstract description 11
- 238000011161 development Methods 0.000 abstract description 3
- 238000009395 breeding Methods 0.000 abstract description 2
- 230000001488 breeding effect Effects 0.000 abstract description 2
- 238000003860 storage Methods 0.000 abstract description 2
- 229940126680 traditional chinese medicines Drugs 0.000 abstract 2
- 239000003962 counterfeit drug Substances 0.000 abstract 1
- 238000005336 cracking Methods 0.000 abstract 1
- 238000003306 harvesting Methods 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 238000005728 strengthening Methods 0.000 abstract 1
- 229940126672 traditional medicines Drugs 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 description 31
- 230000009182 swimming Effects 0.000 description 18
- 239000000243 solution Substances 0.000 description 11
- 239000003550 marker Substances 0.000 description 8
- 244000192528 Chrysanthemum parthenium Species 0.000 description 4
- 235000000604 Chrysanthemum parthenium Nutrition 0.000 description 4
- FBMORZZOJSDNRQ-GLQYFDAESA-N Atractylenolide III Chemical compound C=C([C@@H]1C2)CCC[C@]1(C)C[C@@]1(O)C2=C(C)C(=O)O1 FBMORZZOJSDNRQ-GLQYFDAESA-N 0.000 description 3
- 241000132011 Atractylodes lancea Species 0.000 description 3
- 240000005250 Chrysanthemum indicum Species 0.000 description 3
- 235000018959 Chrysanthemum indicum Nutrition 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 241000628997 Flos Species 0.000 description 3
- 241000935235 Fritillaria meleagris Species 0.000 description 3
- 244000020551 Helianthus annuus Species 0.000 description 3
- 235000003222 Helianthus annuus Nutrition 0.000 description 3
- 235000000802 Leonurus cardiaca ssp. villosus Nutrition 0.000 description 3
- 241000245665 Taraxacum Species 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 2
- 229960005542 ethidium bromide Drugs 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000092665 Atractylodes macrocephala Species 0.000 description 1
- 235000008495 Chrysanthemum leucanthemum Nutrition 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000739 chaotic effect Effects 0.000 description 1
- 229940126678 chinese medicines Drugs 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 235000008384 feverfew Nutrition 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510181371.9A CN104774948B (en) | 2015-04-16 | 2015-04-16 | PCR (Polymerase chain reaction) identification kit and identification method for largehead atractylode in Hebei |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510181371.9A CN104774948B (en) | 2015-04-16 | 2015-04-16 | PCR (Polymerase chain reaction) identification kit and identification method for largehead atractylode in Hebei |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104774948A true CN104774948A (en) | 2015-07-15 |
CN104774948B CN104774948B (en) | 2017-04-12 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510181371.9A Active CN104774948B (en) | 2015-04-16 | 2015-04-16 | PCR (Polymerase chain reaction) identification kit and identification method for largehead atractylode in Hebei |
Country Status (1)
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CN (1) | CN104774948B (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108588260A (en) * | 2018-05-15 | 2018-09-28 | 浙江工业大学 | Osmanthus Radix Curcumae PCR identification kits and identification method |
CN108624708A (en) * | 2018-05-15 | 2018-10-09 | 浙江工业大学 | Green silk Radix Curcumae PCR identification kits and identification method |
CN114032328A (en) * | 2021-11-26 | 2022-02-11 | 浙江工业大学 | Huaijuan PCR identification kit and application thereof |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101230389A (en) * | 2007-12-05 | 2008-07-30 | 浙江大学 | Chinese medicine atractylodes polymerase chain reaction identification primers and identification method |
CN104073553A (en) * | 2014-04-18 | 2014-10-01 | 浙江工业大学 | Hubei radix ophiopogonis PCR (Polymerase Chain Reaction) identification kit and identification method |
-
2015
- 2015-04-16 CN CN201510181371.9A patent/CN104774948B/en active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101230389A (en) * | 2007-12-05 | 2008-07-30 | 浙江大学 | Chinese medicine atractylodes polymerase chain reaction identification primers and identification method |
CN104073553A (en) * | 2014-04-18 | 2014-10-01 | 浙江工业大学 | Hubei radix ophiopogonis PCR (Polymerase Chain Reaction) identification kit and identification method |
Non-Patent Citations (2)
Title |
---|
朱振洪 等: "四种不同产地白术的DNA随机扩增多态性研究", 《浙江中医药大学学报》 * |
王宗宽 等: "白术不同居群亲缘关系RAPD分析", 《安徽林业科技》 * |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108588260A (en) * | 2018-05-15 | 2018-09-28 | 浙江工业大学 | Osmanthus Radix Curcumae PCR identification kits and identification method |
CN108624708A (en) * | 2018-05-15 | 2018-10-09 | 浙江工业大学 | Green silk Radix Curcumae PCR identification kits and identification method |
CN108588260B (en) * | 2018-05-15 | 2019-11-29 | 浙江工业大学 | Osmanthus Radix Curcumae PCR identification kit and identification method |
CN108624708B (en) * | 2018-05-15 | 2019-11-29 | 浙江工业大学 | Green silk Radix Curcumae PCR identification kit and identification method |
CN114032328A (en) * | 2021-11-26 | 2022-02-11 | 浙江工业大学 | Huaijuan PCR identification kit and application thereof |
CN114032328B (en) * | 2021-11-26 | 2023-08-08 | 浙江工业大学 | Huaihu PCR identification kit and application |
Also Published As
Publication number | Publication date |
---|---|
CN104774948B (en) | 2017-04-12 |
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C06 | Publication | ||
PB01 | Publication | ||
EXSB | Decision made by sipo to initiate substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20201010 Address after: 221300 north side of Qingnian East Road, Yunhe Town, Pizhou City, Xuzhou City, Jiangsu Province Patentee after: Pizhou Runhong Industry Co.,Ltd. Address before: 510000 unit 2414-2416, building, No. five, No. 371, Tianhe District, Guangdong, China Patentee before: GUANGDONG GAOHANG INTELLECTUAL PROPERTY OPERATION Co.,Ltd. Effective date of registration: 20201010 Address after: 510000 unit 2414-2416, building, No. five, No. 371, Tianhe District, Guangdong, China Patentee after: GUANGDONG GAOHANG INTELLECTUAL PROPERTY OPERATION Co.,Ltd. Address before: 310014 Hangzhou city in the lower reaches of the city of Zhejiang Wang Road, No. 18 Patentee before: ZHEJIANG University OF TECHNOLOGY |
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Effective date of registration: 20240715 Address after: 221300 north side of Jiefang East Road, Pizhou City, Xuzhou City, Jiangsu Province Patentee after: Xuzhou Jiasheng Real Estate Co.,Ltd. Country or region after: China Address before: 221300 north side of Qingnian East Road, Yunhe Town, Pizhou City, Xuzhou City, Jiangsu Province Patentee before: Pizhou Runhong Industry Co.,Ltd. Country or region before: China |