CN104770624A - Application of salicylate decarboxylase in degradation of ginkgoic acid - Google Patents
Application of salicylate decarboxylase in degradation of ginkgoic acid Download PDFInfo
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- CN104770624A CN104770624A CN201510052113.0A CN201510052113A CN104770624A CN 104770624 A CN104770624 A CN 104770624A CN 201510052113 A CN201510052113 A CN 201510052113A CN 104770624 A CN104770624 A CN 104770624A
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- ginkgoic acid
- acid
- ginkgoic
- decarboxylase
- sdc
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- YXHVCZZLWZYHSA-FPLPWBNLSA-N Ginkgoic acid Chemical compound CCCCCC\C=C/CCCCCCCC1=CC=CC(O)=C1C(O)=O YXHVCZZLWZYHSA-FPLPWBNLSA-N 0.000 title claims abstract description 118
- 230000015556 catabolic process Effects 0.000 title abstract description 7
- 238000006731 degradation reaction Methods 0.000 title abstract description 7
- 108030003432 Salicylate decarboxylases Proteins 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 9
- 101000821475 Cutaneotrichosporon moniliiforme Salicylate decarboxylase Proteins 0.000 claims description 16
- 241000588724 Escherichia coli Species 0.000 claims description 8
- 241000894006 Bacteria Species 0.000 claims description 7
- 238000010353 genetic engineering Methods 0.000 claims description 4
- 239000013600 plasmid vector Substances 0.000 claims description 2
- 239000007788 liquid Substances 0.000 abstract description 9
- 241000218628 Ginkgo Species 0.000 abstract description 7
- 235000011201 Ginkgo Nutrition 0.000 abstract description 7
- 235000008100 Ginkgo biloba Nutrition 0.000 abstract description 7
- 102000004190 Enzymes Human genes 0.000 abstract description 6
- 108090000790 Enzymes Proteins 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 2
- 241001198387 Escherichia coli BL21(DE3) Species 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 238000013467 fragmentation Methods 0.000 abstract 1
- 238000006062 fragmentation reaction Methods 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 description 14
- 238000000034 method Methods 0.000 description 10
- 101150025902 SDC gene Proteins 0.000 description 9
- 239000000243 solution Substances 0.000 description 8
- 230000029087 digestion Effects 0.000 description 7
- 108010042407 Endonucleases Proteins 0.000 description 4
- 102000004533 Endonucleases Human genes 0.000 description 4
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 238000012797 qualification Methods 0.000 description 3
- 239000012086 standard solution Substances 0.000 description 3
- YXHVCZZLWZYHSA-UHFFFAOYSA-N (Z)-6-[8-pentadecenyl]salicylic acid Natural products CCCCCCC=CCCCCCCCC1=CC=CC(O)=C1C(O)=O YXHVCZZLWZYHSA-UHFFFAOYSA-N 0.000 description 2
- 238000006065 biodegradation reaction Methods 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 2
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 108010005054 Deoxyribonuclease BamHI Proteins 0.000 description 1
- 108010029541 Laccase Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 230000006652 catabolic pathway Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000005008 domestic process Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 235000019225 fermented tea Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 239000006193 liquid solution Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000012422 test repetition Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000002525 ultrasonication Methods 0.000 description 1
- 238000009777 vacuum freeze-drying Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to an application of salicylate decarboxylase in degradation of ginkgoic acid. The gene sequence of the above enzyme is represented by sequence 1, the above enzyme is salicylate decarboxylase (Sdc), and an Sdc-containing pET21a(+)-sdc/E. coli BL21(DE3) cell fragmentation liquid can obviously reduce the content of ginkgoic acid. The enzyme has a ginkgoic acid degradation effect in wide temperature and pH value ranges, provides a fast and convenient method for extraction of ginkgoic acid from ginkgo, and gives more possibilities for wide utilization of ginkgo.
Description
Technical field
The invention belongs to biological technical field, relate to a kind of salicylic acid decarboxylase (Salicylic acid decarboxylase, the Sdc) application to the degraded of ginkgoic acid.
Background technology
Ginkgo is very wide and with a long history in China's distribution, and there is very high using value at its position such as leaf, seed.But at a kind of toxic component---ginkgoic acid of these position ubiquities.
Ginkgoic acid is a class septichen compounds, comprises C13:1, C15:1, C17:2, C15:0, C17:1 etc., has very large harm to human body.
Physical removal methods and chemical minimizing technology are the common methods of degraded ginkgoic acid, external bibliographical information less, the domestic method such as microwave and supercritical extract that once used removes ginkgoic acid, and these methods all need to utilize organic solvent to dissolve ginkgoic acid, complex process.In biodegradation, the people such as Li Yinliang ginkgoic acid in artificial fermentation's ginkgo leaf made by the artificial infection coronoid process capsule bacterium fermentation ginkgo leaf that falls apart the content that fermented tea can reduce wherein ginkgoic acid containing once reporting in quantifier elimination, but consuming time longer, and step is more loaded down with trivial details.
By retrieval, find one section of patent document relevant to present patent application: a kind of biodegradation method (CN103053876) of ginkgolic acid, the method is the (PH4.0-6.0 of degraded system while the laccase LacC adding 0.004U/ml-0.006U/ml in the degeneration system containing finite concentration ginkgolic acid, degradation temperature is 45 DEG C-60 DEG C, the degradation treatment time is 10-24h), add amboceptor material, particularly add ABTS, degradation rate reaches as high as 100%.
By contrast, above-mentioned disclosed document and present patent application have relatively big difference in technical scheme, and the technique effect of acquisition is different.
Summary of the invention
The object of the present invention is to provide the application of a kind of enzyme degraded ginkgoic acid, the degradation pathway of another kind of ginkgoic acid is provided.
The technical scheme that the present invention realizes object is:
A kind of application of salicylic acid decarboxylase degraded ginkgoic acid.The gene order of described salicylic acid decarboxylase is as described in sequence 1.
The temperature of described salicylic acid decarboxylase explanation ginkgoic acid is 5 ~ 80 DEG C, and pH is 3.0 ~ 11.0.
Described ginkgoic acid is for comprising ginkgoic acid R=C13:0, ginkgoic acid R=C15:0, ginkgoic acid R=C15:1, ginkgoic acid R=C17:1, ginkgoic acid R=C17:2.
To degrade the genetic engineering bacterium of ginkgoic acid, containing the salicylic acid decarboxylase of sequence as described in sequence 1.
Described plasmid vector is pET21a (+).
Described Host Strains is E.coli BL21 (DE3).
Advantage of the present invention and beneficial effect are:
1, the invention provides a kind of enzyme of ginkgoic acid of can degrading, this enzyme is salicylic acid decarboxylase Sdc, and the clasmatosis liquid of pET21a (+)-sdc/E.coli BL21 (DE3) containing Sdc obviously can reduce the content of ginkgoic acid.Its clasmatosis liquid of 1mL 40 DEG C, make the content of ginkgoic acid C15:1 reduce 39.017 ± 0.131% in pH=5.5,5h.
2, salicylic acid decarboxylase provided by the invention can play the degradation to ginkgoic acid within the scope of wider temperature and pH, provides more quick method easily, for the extensive utilization of ginkgo provides more possibilities for taking out ginkgoic acid in ginkgo.
Accompanying drawing explanation
Fig. 1 is the structure of recombinant plasmid pET21a (+)-sdc;
Fig. 2 is the qualification of pET21a (+)-sdc recombinant plasmid; The single endonuclease digestion result of M:maker (10kb), 1:pET21a (+)-sdc, the double digestion result of 2:pET21a (+)-sdc;
Fig. 3 is the qualification of control group pET21a (+) recombinant plasmid.The single endonuclease digestion result of M:maker (10kb), 1:pET21a (+);
Detailed description of the invention
Below by specific embodiment, the invention will be further described, and following examples are descriptive, is not determinate, can not limit protection scope of the present invention with this.
The gene order that the present invention reports according to Kirimura etc., Prof. Du Yucang sdc gene, and build the recombinant plasmid of this gene, then proceeded in host cell, recombinant bacterium is inoculated in culture medium and cultivates certain hour, therefrom get a certain amount of zymotic fluid and carry out clasmatosis.Take a certain amount of ginkgoic acid standard items, be configured to solution.After clasmatosis liquid and standard solution mixing, reaction a period of time, with LC-MS mensuration ginkgoic acid content wherein.
Wherein, ginkgoic acid refers to a class 2-hydroxyl-6-alkylbenzoic acid compounds, comprises ginkgoic acid (R=C13:0), ginkgoic acid (R=C15:0), ginkgoic acid (R=C15:1) ginkgoic acid (R=C17:1) ginkgoic acid (R=C17:2) etc.
Concrete operations mode and step as follows:
(1) according to the gene order that Kirimura etc. reports, Prof. Du Yucang sdc gene, and build recombinant plasmid pET21a (+)-sdc of this gene, then to be proceeded in E.coli BL21 (DE3).
Use HindIII/Nde I to carry out double digestion to sdc gene and plasmid pET21a (+) respectively, get endonuclease bamhi and carry out coupled reaction, then proceed to the structure (as Fig. 1) that E.coli BL21 (DE3) has carried out plasmid.By nutrient solution coating Amp resistant panel, recombinant plasmid pET21a (+)-sdc contains the resistant gene of Amp, therefore make the BL21 proceeding to this recombinant plasmid also with Amp resistance, herein according to whether screening recon with Amp resistance, what Amp resistant panel can grow is exactly the recon successfully constructed.From LB solid plate, picking list bacterium colony, is inoculated in LB liquid medium after incubated overnight, extracts plasmid, is identified by agarose gel electrophoresis.Result is as Fig. 2.
There are two bands in double digestion recombinant plasmid, is respectively 5443bp and 1053bp, in the same size with plasmid pET21a (+) and sdc gene, and a band appears in single endonuclease digestion recombinant plasmid, is 6496bp.Checked order by correct recombinant plasmid through checking, the sequence obtained is identical with former sequence, illustrates that carrier pET21a (+)-sdc determines to successfully construct.
Due to the resistant gene of pET21a (+) containing Amp, the screening of recon is carried out so can use the same method, Fig. 3 is the qualification result of control group single endonuclease digestion, a band is there is in figure, for 5443bp, illustrate that pET21a (+) successfully proceeds to E.coli BL21 (DE3).
(2) pET21a (+)-sdc/E.coli BL21 (DE3) of restructuring being inoculated into Amp and IPTG concentration is in the LB culture medium of 100 μ g/mg, at 37 DEG C of temperature, 130rpm cultivates 12h, nutrient solution is inoculated in IPTG and Amp concentration by 1% inoculum concentration and is 100 μ gmL
-1lB culture medium in, 30 DEG C, 120rpm cultivates 18h.
(3) get step nutrient solution 4mL (2), ultrasonication in ice bath, power 40%, work 3s, interval 5s, the centrifugal 30s of time 10min, 3000rpm, abandons supernatant, then adds 2mL PBS buffer solution, can obtain clasmatosis liquid after mixing.
(4) mixed in the centrifuge tube A of 10mL by the dilution 1mL of clasmatosis liquid and ginkgoic acid C15:1 standard solution, each 1mL of dilution getting PBS buffer solution and ginkgoic acid C15:1 standard solution mixes in contrast in the centrifuge tube B of 10mL.O is passed in two pipes
2about 5min, in 40 DEG C, pH=5.5 reacts 5h, by reacted each group of solution for vacuum freeze drying to constant weight (double weighing result difference is no more than 0.002g), add 2mL isopropyl alcohol respectively wherein again, abundant dissolving, with 0.22 μm of organic membrane filtration, and measure the ginkgoic acid C15:1 concentration after each group reaction in solution with LC-MS, often group test repetition 3 times.Can calculate with the ginkgoic acid content in the reacted solution of clasmatosis liquid is 0.003 ± 0.045mg/mL, and the ginkgoic acid content in control group solution is 0.006 ± 0.042mg/mL.
The content of ginkgoic acid obviously can be reduced according to the clasmatosis liquid of known pET21a (+)-sdc/E.coli BL21 (DE3) containing Sdc of the result of embodiment.Its clasmatosis liquid of 1mL 40 DEG C, make the content of ginkgoic acid C15:1 reduce 39.017 ± 0.131% in pH=5.5,5h.
The sequence of described sdc gene is:
Claims (7)
1. the application of a salicylic acid decarboxylase degraded ginkgoic acid.
2. the application of salicylic acid decarboxylase degraded ginkgoic acid according to claim 1, is characterized in that: the gene order of described salicylic acid decarboxylase is as described in sequence 1.
3. the application of salicylic acid decarboxylase degraded ginkgoic acid according to claim 1, is characterized in that: the temperature of described salicylic acid decarboxylase explanation ginkgoic acid is 5 ~ 80 DEG C, and pH is 3.0 ~ 11.0.
4. the application of salicylic acid decarboxylase degraded ginkgoic acid according to claim 1, is characterized in that: described ginkgoic acid is for comprising ginkgoic acid R=C13:0, ginkgoic acid R=C15:0, ginkgoic acid R=C15:1, ginkgoic acid R=C17:1, ginkgoic acid R=C17:2.
5. to degrade the genetic engineering bacterium of ginkgoic acid, it is characterized in that: containing the salicylic acid decarboxylase of sequence as described in sequence 1.
6. the genetic engineering bacterium of degraded ginkgoic acid according to claim 1, is characterized in that: described plasmid vector is pET21a (+).
7. the genetic engineering bacterium of degraded ginkgoic acid according to claim 1, is characterized in that: described Host Strains is E.coli BL21 (DE3).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510052113.0A CN104770624B (en) | 2015-02-02 | 2015-02-02 | A kind of application of bigcatkin willow acid decarboxylase degraded ginkgoic acid |
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| CN201510052113.0A CN104770624B (en) | 2015-02-02 | 2015-02-02 | A kind of application of bigcatkin willow acid decarboxylase degraded ginkgoic acid |
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| CN104770624B CN104770624B (en) | 2017-12-12 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106916855A (en) * | 2017-03-01 | 2017-07-04 | 天津科技大学 | The method modified aldehydes matter using carbon dioxide bioconversion method and application |
| CN110205316A (en) * | 2019-06-21 | 2019-09-06 | 济宁学院 | A kind of method of enzymic degradation 4-ASA |
| CN110241102A (en) * | 2019-06-21 | 2019-09-17 | 济宁学院 | A kind of method of enzymic degradation 2,6- dihydroxy-benzoic acid |
| CN110839644A (en) * | 2019-11-27 | 2020-02-28 | 中南林业科技大学 | Method for improving effective and economic yield of ginkgolic acid from ginkgo biloba sarcotesta |
| CN114645039A (en) * | 2022-04-26 | 2022-06-21 | 天津科技大学 | Mutant salicylate decarboxylase, strain and application thereof in degradation of ginkgolic acid |
| CN116376936A (en) * | 2023-06-01 | 2023-07-04 | 青岛农业大学 | Method for biosynthesis of ginkgolic acid and gene sequence thereof |
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| JP2008200010A (en) * | 2007-02-22 | 2008-09-04 | Univ Waseda | New microorganism having ability to synthesize aromatic hydroxycarboxylic acid, and method for producing aromatic hydroxycarboxylic acid using the above microorganism or protein produced by the same |
| CN103053876A (en) * | 2012-12-17 | 2013-04-24 | 南京林业大学 | Biodegradation method of ginkgolic acid |
| CN103960555A (en) * | 2014-04-11 | 2014-08-06 | 天津科技大学 | Method for removing ginkgolic acid in gingko biloba seeds |
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2015
- 2015-02-02 CN CN201510052113.0A patent/CN104770624B/en not_active Expired - Fee Related
Patent Citations (3)
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| JP2008200010A (en) * | 2007-02-22 | 2008-09-04 | Univ Waseda | New microorganism having ability to synthesize aromatic hydroxycarboxylic acid, and method for producing aromatic hydroxycarboxylic acid using the above microorganism or protein produced by the same |
| CN103053876A (en) * | 2012-12-17 | 2013-04-24 | 南京林业大学 | Biodegradation method of ginkgolic acid |
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Non-Patent Citations (1)
| Title |
|---|
| KOHTARO KIRIMURA ET.AL: "Enzymatic Kolbe-Schmitt reaction to form salicylic acid from phenol:Enzymatic characterization and gene identification of a novel enzyme,Trichosporon moniliiforme salicylic acid decarboxylase", 《BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106916855A (en) * | 2017-03-01 | 2017-07-04 | 天津科技大学 | The method modified aldehydes matter using carbon dioxide bioconversion method and application |
| CN110205316A (en) * | 2019-06-21 | 2019-09-06 | 济宁学院 | A kind of method of enzymic degradation 4-ASA |
| CN110241102A (en) * | 2019-06-21 | 2019-09-17 | 济宁学院 | A kind of method of enzymic degradation 2,6- dihydroxy-benzoic acid |
| CN110839644A (en) * | 2019-11-27 | 2020-02-28 | 中南林业科技大学 | Method for improving effective and economic yield of ginkgolic acid from ginkgo biloba sarcotesta |
| CN114645039A (en) * | 2022-04-26 | 2022-06-21 | 天津科技大学 | Mutant salicylate decarboxylase, strain and application thereof in degradation of ginkgolic acid |
| CN114645039B (en) * | 2022-04-26 | 2024-02-23 | 天津科技大学 | Mutant salicylic acid decarboxylase, strain and application thereof in degradation of ginkgolic acid |
| CN116376936A (en) * | 2023-06-01 | 2023-07-04 | 青岛农业大学 | Method for biosynthesis of ginkgolic acid and gene sequence thereof |
| CN116376936B (en) * | 2023-06-01 | 2023-08-22 | 青岛农业大学 | Method for biosynthesis of ginkgolic acid and gene sequence thereof |
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