CN104770463B - A kind of composite biological preservative suitable for seashell products - Google Patents
A kind of composite biological preservative suitable for seashell products Download PDFInfo
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- 239000003755 preservative agent Substances 0.000 title claims abstract description 26
- 239000002131 composite material Substances 0.000 title claims abstract description 23
- 230000002335 preservative effect Effects 0.000 title claims abstract description 23
- 239000000843 powder Substances 0.000 claims abstract description 32
- 239000002904 solvent Substances 0.000 claims abstract description 31
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 30
- 108010053775 Nisin Proteins 0.000 claims abstract description 27
- NVNLLIYOARQCIX-MSHCCFNRSA-N Nisin Chemical compound N1C(=O)[C@@H](CC(C)C)NC(=O)C(=C)NC(=O)[C@@H]([C@H](C)CC)NC(=O)[C@@H](NC(=O)C(=C/C)/NC(=O)[C@H](N)[C@H](C)CC)CSC[C@@H]1C(=O)N[C@@H]1C(=O)N2CCC[C@@H]2C(=O)NCC(=O)N[C@@H](C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(NCC(=O)N[C@H](C)C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCSC)C(=O)NCC(=O)N[C@H](CS[C@@H]2C)C(=O)N[C@H](CC(N)=O)C(=O)N[C@H](CCSC)C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(N[C@H](C)C(=O)N[C@@H]3C(=O)N[C@@H](C(N[C@H](CC=4NC=NC=4)C(=O)N[C@H](CS[C@@H]3C)C(=O)N[C@H](CO)C(=O)N[C@H]([C@H](C)CC)C(=O)N[C@H](CC=3NC=NC=3)C(=O)N[C@H](C(C)C)C(=O)NC(=C)C(=O)N[C@H](CCCCN)C(O)=O)=O)CS[C@@H]2C)=O)=O)CS[C@@H]1C NVNLLIYOARQCIX-MSHCCFNRSA-N 0.000 claims abstract description 27
- 239000004309 nisin Substances 0.000 claims abstract description 27
- 235000010297 nisin Nutrition 0.000 claims abstract description 27
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 235000010298 natamycin Nutrition 0.000 claims abstract description 16
- 239000004311 natamycin Substances 0.000 claims abstract description 16
- NCXMLFZGDNKEPB-FFPOYIOWSA-N natamycin Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C[C@@H](C)OC(=O)/C=C/[C@H]2O[C@@H]2C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 NCXMLFZGDNKEPB-FFPOYIOWSA-N 0.000 claims abstract description 16
- 229960003255 natamycin Drugs 0.000 claims abstract description 16
- 235000019832 sodium triphosphate Nutrition 0.000 claims abstract description 15
- 229920001661 Chitosan Polymers 0.000 claims abstract description 14
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 14
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 14
- 238000013329 compounding Methods 0.000 claims abstract description 14
- 239000000203 mixture Substances 0.000 claims abstract description 14
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 14
- 239000011718 vitamin C Substances 0.000 claims abstract description 14
- 239000002504 physiological saline solution Substances 0.000 claims abstract description 13
- 210000001367 artery Anatomy 0.000 claims 1
- 210000003462 vein Anatomy 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 abstract description 2
- 102000004190 Enzymes Human genes 0.000 abstract description 2
- 241000588724 Escherichia coli Species 0.000 abstract description 2
- 241000191967 Staphylococcus aureus Species 0.000 abstract description 2
- 230000001408 fungistatic effect Effects 0.000 abstract description 2
- 241000228245 Aspergillus niger Species 0.000 abstract 1
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 abstract 1
- RGHNJXZEOKUKBD-SQOUGZDYSA-N Gluconic acid Natural products OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 abstract 1
- 235000012208 gluconic acid Nutrition 0.000 abstract 1
- 239000000174 gluconic acid Substances 0.000 abstract 1
- 235000013305 food Nutrition 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000005260 corrosion Methods 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 239000000796 flavoring agent Substances 0.000 description 3
- 235000019634 flavors Nutrition 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000003260 anti-sepsis Effects 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 241000228212 Aspergillus Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 239000005913 Maltodextrin Substances 0.000 description 1
- 229920002774 Maltodextrin Polymers 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- IMNFDUFMRHMDMM-UHFFFAOYSA-N N-Heptane Chemical compound CCCCCCC IMNFDUFMRHMDMM-UHFFFAOYSA-N 0.000 description 1
- 108010039918 Polylysine Proteins 0.000 description 1
- QNVSXXGDAPORNA-UHFFFAOYSA-N Resveratrol Natural products OC1=CC=CC(C=CC=2C=C(O)C(O)=CC=2)=C1 QNVSXXGDAPORNA-UHFFFAOYSA-N 0.000 description 1
- 239000004288 Sodium dehydroacetate Substances 0.000 description 1
- 244000057717 Streptococcus lactis Species 0.000 description 1
- 235000014897 Streptococcus lactis Nutrition 0.000 description 1
- LUKBXSAWLPMMSZ-OWOJBTEDSA-N Trans-resveratrol Chemical compound C1=CC(O)=CC=C1\C=C\C1=CC(O)=CC(O)=C1 LUKBXSAWLPMMSZ-OWOJBTEDSA-N 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 108010027597 alpha-chymotrypsin Proteins 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 229940064004 antiseptic throat preparations Drugs 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- BEGBSFPALGFMJI-UHFFFAOYSA-N ethene;sodium Chemical group [Na].C=C BEGBSFPALGFMJI-UHFFFAOYSA-N 0.000 description 1
- 229960001617 ethyl hydroxybenzoate Drugs 0.000 description 1
- 235000010228 ethyl p-hydroxybenzoate Nutrition 0.000 description 1
- 239000004403 ethyl p-hydroxybenzoate Substances 0.000 description 1
- NUVBSKCKDOMJSU-UHFFFAOYSA-N ethylparaben Chemical compound CCOC(=O)C1=CC=C(O)C=C1 NUVBSKCKDOMJSU-UHFFFAOYSA-N 0.000 description 1
- 235000019249 food preservative Nutrition 0.000 description 1
- 239000005452 food preservative Substances 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 229940035034 maltodextrin Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 229940077082 red pepper extract Drugs 0.000 description 1
- 235000021283 resveratrol Nutrition 0.000 description 1
- 229940016667 resveratrol Drugs 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 235000019259 sodium dehydroacetate Nutrition 0.000 description 1
- 229940079839 sodium dehydroacetate Drugs 0.000 description 1
- GCLGEJMYGQKIIW-UHFFFAOYSA-H sodium hexametaphosphate Chemical compound [Na]OP1(=O)OP(=O)(O[Na])OP(=O)(O[Na])OP(=O)(O[Na])OP(=O)(O[Na])OP(=O)(O[Na])O1 GCLGEJMYGQKIIW-UHFFFAOYSA-H 0.000 description 1
- DSOWAKKSGYUMTF-GZOLSCHFSA-M sodium;(1e)-1-(6-methyl-2,4-dioxopyran-3-ylidene)ethanolate Chemical compound [Na+].C\C([O-])=C1/C(=O)OC(C)=CC1=O DSOWAKKSGYUMTF-GZOLSCHFSA-M 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
A kind of composite biological preservative suitable for seashell products, it is made up of dry powder and solvent, it is characterised in that the parts by weight composition of the dry powder is as follows:9 20 parts of natamycin;30 100 parts of nisin;11 19 parts of subtilicin;10 50 parts of vitamin C;10 180 parts of chitosan;50 90 parts of sodium tripolyphosphate;60 70 parts of gluconic acid δ lactones;Solvent is 0.9% physiological saline, and dry powder and solvent are according to solid-liquid ratio 1:1000 compoundings, are placed under the 12v pulse powers after well mixed and are powered 25 minutes, interpulse period is 1 2s.Composite biological preservative of the present invention, the more commercially available common preservatives of fungistatic effect of staphylococcus aureus (ATCC6538), Escherichia coli (ATCC8739), aspergillus niger (ATCC16404), the false silk enzyme of white female (ATCC10231) are more than doubled.
Description
Technical field
The invention belongs to the technical field of preservative, and in particular to a kind of compound bio anti-corrosion suitable for seashell products
Agent.
Background technology
Nisin(Nisin)Also known as nisin or transliteration are that Buddhist nun is pungent, are one caused by streptococcus lactis
Kind peptide material, is made up of, molecular weight is about 3500 Da 34 amino acid residues.Because nisin can suppress most
Number gram-positive bacterium, and have strong inhibitory action to the spore of bacillus, therefore by extensive as food preservative
Applied to food service industry.Amino is hydrolyzed into quickly under physiological pH condition and α-chymotrypsin protein enzyme effect of human body after edible
Acid, normal flora in human body intestinal canal will not be changed and produce the resistance problem occurred such as other antibiotic, more will not be with it
There is cross tolerance in its antibiotic, be it is a kind of it is efficient, nontoxic, safely, the antiseptics for natural food that has no side effect.
Composite biological preservative based on nisin, it is a kind of as disclosed in Chinese patent literature CN 101167595
Natamycin nisin composite biological antisepsis emulsion includes:Natamycin 1-8 parts;Nisin 15-150 parts;
Vitamin C 10-50 parts, sodium ethylene diamine tetracetate 55-90 parts;Maltodextrin 10-180 parts;Sodium tripolyphosphate 100-1000 parts;
Calgon 100-1000 parts (weight ratio);A kind of composite preservative disclosed in Chinese patent literature CN 101779698A, should
Composite preservative is prepared by following raw materials by its weight ratio:Nisin 5~20, sodium dehydroacetate 10~60, Portugal
Grape saccharic acid-δ-lactones 5~50, different Vc sodium 5~40, sodium chloride 5~50, sodium tripolyphosphate 1~50.Chinese patent literature CN
104082830A discloses a kind of compound bio antisepsis antistaling agent, the constituent containing following parts by weight:Epsilon-polylysine 10-
40 parts, nisin 5-30 parts, natamycin 1-10 parts, lysozyme 0.01-0.5 parts, subtilicin 1-10 parts,
Ascorbic acid 5-20 parts, red pepper extract 0.5-2 parts, water-soluble resveratrol preparation 0.05-0.5 parts, Tea Polyphenols 1-10 parts,
Chitosan 2-10 parts.Chinese patent literature CN 101589839 discloses composite biological preservative and its preparation side of a kind of food
Method, it is related to and preserves food with microorganism, the biological preservative is made up of nisin, pyrolkigneous liquid and distilled water, per 100ML
It is distilled water to contain 350~400MG of nisin, 15~25ML of pyrolkigneous liquid and remaining in the composite biological preservative of food;
Its compound method is:350~400MG of extracting lactic acid streptostacin and 15~25ML of pyrolkigneous liquid mixing, then be settled to distilled water
100ML.Etc. many composite biological preservatives based on nisin emerge.
But inventor find, above-mentioned composite biological preservative mostly exist inactivation the out-of-service time it is short the problem of, furthermore by
Just only there is higher heat resistance, therefore above-mentioned composite biological preservative in the case where pH is less than 2 in nisin
In order to heat-resisting, solution environmental is set and reached or close to pH value 2.
Inventor's research show that, because seashell products protein content is higher, the composition such as remaining lipid is relatively low, higher
Under pH environment, flavor substance easily fails, and have impact on mouthfeel and flavor.
The content of the invention
The present invention is directed to above-mentioned the deficiencies in the prior art, there is provided a kind of compound bio anti-corrosion suitable for seashell products
Agent, has a relatively low pH, heat-resisting, Stability Analysis of Structures, and the out-of-service time is longer.
The present invention technology path be:
A kind of composite biological preservative suitable for seashell products, it is made up of dry powder and solvent, it is characterised in that described dry
The parts by weight composition of powder is as follows:
Natamycin 9-20 parts;Nisin 30-100 parts;Subtilicin 11-19 parts;Vitamin C 10-
50 parts;Chitosan 10-180 parts;Sodium tripolyphosphate 50-90 parts;Gluconic acid-δ-lactones 60-70 parts;
Solvent is 0.9% physiological saline,
Dry powder and solvent are according to solid-liquid ratio 1:1000 compoundings, energization 2-5 points are placed under the 12v pulse powers after well mixed
Clock, interpulse period 1-2s.
Optimally, the parts by weight composition of the dry powder is as follows:
Natamycin 9-10 parts;Nisin 30-50 parts;Subtilicin 11-16 parts;Vitamin C 10-
25 parts, chitosan 10-60 parts;Sodium tripolyphosphate 50-70 parts;Gluconic acid-δ-lactones 60-65 parts;
Solvent is 0.9% physiological saline,
Dry powder and solvent are according to solid-liquid ratio 1:1000 compoundings, are placed under the 12v pulse powers after well mixed and are powered 3 minutes,
Interpulse period is 2s.
Optimally, the parts by weight composition of the dry powder is as follows:
Natamycin 10-20 parts;Nisin 50-100 parts;Subtilicin 16-19 parts;Vitamin C
25-50 parts, chitosan 60-180 parts;Sodium tripolyphosphate 70-90 parts;Gluconic acid-δ-lactones 65-70 parts;
Solvent is 0.9% physiological saline,
Dry powder and solvent are according to solid-liquid ratio 1:1000 compoundings, are placed under the 12v pulse powers after well mixed and are powered 5 minutes,
Interpulse period is 1s.
The composite biological preservative for compounding gained is placed under the pulse power unintentionally by inventor, by putting for appropriate time
Electricity, it is surprisingly found that, the significant effect in seashell products anti-corrosion of the composite biological preservative after energization, and does not influence shellfish production
Itself flavor of product.Composite biological preservative pH value after energization is changed into 4.5 or so from original 2.3 or so, is detected in Electronic Speculum
Substantial variation does not occur for the lower stereochemical structure for finding nisin, but above-mentioned dry powder component forms certain chelating
Thing.
Composite biological preservative of the present invention, to staphylococcus aureus (ATCC6538), Escherichia coli (ATCC8739), black
Aspergillus (ATCC16404), the more commercially available common preservatives of fungistatic effect of the false silk enzyme of white female (ATCC10231) be doubled with
On.
Embodiment
Embodiment 1
A kind of composite biological preservative suitable for seashell products, it is made up of dry powder and solvent, it is characterised in that described dry
The parts by weight composition of powder is as follows:
9 parts of natamycin;30 parts of nisin;11 parts of subtilicin;10 parts of vitamin C;Chitosan 10
Part;50 parts of sodium tripolyphosphate;60 parts of gluconic acid-δ-lactones;
Solvent is 0.9% physiological saline,
Dry powder and solvent are according to solid-liquid ratio 1:1000 compoundings, are placed under the 12v pulse powers after well mixed and are powered 2 minutes,
Interpulse period is 1s.
Embodiment 2
A kind of composite biological preservative suitable for seashell products, it is made up of dry powder and solvent, it is characterised in that described dry
The parts by weight composition of powder is as follows:
20 parts of natamycin;100 parts of nisin;19 parts of subtilicin;50 parts of vitamin C;Chitosan
180 parts;90 parts of sodium tripolyphosphate;70 parts of gluconic acid-δ-lactones;
Solvent is 0.9% physiological saline,
Dry powder and solvent are according to solid-liquid ratio 1:1000 compoundings, are placed under the 12v pulse powers after well mixed and are powered 5 minutes,
Interpulse period is 2s.
Embodiment 3
The parts by weight composition of the dry powder is as follows:
10 parts of natamycin;50 parts of nisin;16 parts of subtilicin;25 parts of vitamin C, chitosan
60 parts;70 parts of sodium tripolyphosphate;65 parts of gluconic acid-δ-lactones;
Solvent is 0.9% physiological saline,
Dry powder and solvent are according to solid-liquid ratio 1:1000 compoundings, are placed under the 12v pulse powers after well mixed and are powered 3 minutes,
Interpulse period is 2s.
Embodiment 4
The parts by weight composition of the dry powder is as follows:
9.5 parts of natamycin;45 parts of nisin;12 parts of subtilicin;18 parts of vitamin C, chitosan
40 parts;60 parts of sodium tripolyphosphate;63 parts of gluconic acid-δ-lactones;
Solvent is 0.9% physiological saline,
Dry powder and solvent are according to solid-liquid ratio 1:1000 compoundings, are placed under the 12v pulse powers after well mixed and are powered 3 minutes,
Interpulse period is 2s.
Embodiment 5
The parts by weight composition of the dry powder is as follows:
9 parts of natamycin;48 parts of nisin;18 parts of subtilicin;19 parts of vitamin C, chitosan 50
Part;65 parts of sodium tripolyphosphate;61 parts of gluconic acid-δ-lactones;
Solvent is 0.9% physiological saline,
Dry powder and solvent are according to solid-liquid ratio 1:1000 compoundings, are placed under the 12v pulse powers after well mixed and are powered 3 minutes,
Interpulse period is 2s.
Embodiment 6
The parts by weight composition of the dry powder is as follows:
9 parts of natamycin;48 parts of nisin;18 parts of subtilicin;17 parts of vitamin C, chitosan 60
Part;70 parts of sodium tripolyphosphate;65 parts of gluconic acid-δ-lactones;10 parts of methyl hydroxybenzoate, 20 parts of ethylparaben, nipalgin heptan
10 parts of ester;
Solvent is 0.9% physiological saline,
Dry powder and solvent are according to solid-liquid ratio 1:1000 compoundings, are placed under the 12v pulse powers after well mixed and are powered 3 minutes,
Interpulse period is 2s.
Claims (3)
1. a kind of composite biological preservative suitable for seashell products, it is made up of dry powder and solvent, it is characterised in that the dry powder
Parts by weight composition it is as follows:
Natamycin 9-20 parts;Nisin 30-100 parts;Subtilicin 11-19 parts;Vitamin C 10-50
Part;Chitosan 10-180 parts;Sodium tripolyphosphate 50-90 parts;Gluconic acid-δ-lactones 60-70 parts;
Solvent is 0.9% physiological saline,
Dry powder and solvent are according to solid-liquid ratio 1:1000 compoundings, energization 2-5 minutes, arteries and veins under the 12v pulse powers are placed in after well mixed
It is 1-2s to rush interval time.
2. the composite biological preservative according to claim 1 suitable for seashell products, it is characterised in that the dry powder
Parts by weight composition is as follows:
Natamycin 9-10 parts;Nisin 30-50 parts;Subtilicin 11-16 parts;Vitamin C 10-25 parts,
Chitosan 10-60 parts;Sodium tripolyphosphate 50-70 parts;Gluconic acid-δ-lactones 60-65 parts;
Solvent is 0.9% physiological saline,
Dry powder and solvent are according to solid-liquid ratio 1:1000 compoundings, are placed under the 12v pulse powers after well mixed and are powered 3 minutes, pulse
Interval time is 2s.
3. the composite biological preservative according to claim 1 suitable for seashell products, it is characterised in that the dry powder
Parts by weight composition is as follows:
Natamycin 10-20 parts;Nisin 50-100 parts;Subtilicin 16-19 parts;Vitamin C 25-50
Part, chitosan 60-180 parts;Sodium tripolyphosphate 70-90 parts;Gluconic acid-δ-lactones 65-70 parts;
Solvent is 0.9% physiological saline,
Dry powder and solvent are according to solid-liquid ratio 1:1000 compoundings, are placed under the 12v pulse powers after well mixed and are powered 5 minutes, pulse
Interval time is 1s.
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CN101167595A (en) * | 2007-12-03 | 2008-04-30 | 北京市科威华食品工程技术有限公司 | Natamycin nisin composite biological preservative |
CN102188034A (en) * | 2010-03-03 | 2011-09-21 | 天津科技大学 | Compound biological antiseptic and preservation agent for food |
CN102524911A (en) * | 2011-12-21 | 2012-07-04 | 戚向阳 | Biological preservative and method for antisepsis and freshness retaining of foods by using the biological preservative |
CN103734251A (en) * | 2013-12-09 | 2014-04-23 | 苏州市相城区新时代特种水产养殖场 | Aquatic product fresh-keeping method |
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