CN104770369A - Kasugamycin water-aqua extracting process - Google Patents
Kasugamycin water-aqua extracting process Download PDFInfo
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- CN104770369A CN104770369A CN201510196286.XA CN201510196286A CN104770369A CN 104770369 A CN104770369 A CN 104770369A CN 201510196286 A CN201510196286 A CN 201510196286A CN 104770369 A CN104770369 A CN 104770369A
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Abstract
The invention relates to a kasugamycin water-aqua extracting process, which comprises the following steps: carrying out plate-frame filtering on streptomyces microaureus fermentation liquor so as to remove mycelia; carrying out continuous centrifugalization on the filtrate; feeding the obtained centrifugate into an ion-exchange column and carrying out ion-exchange, and desorbing the obtained product at room temperature by using ammonium chloride saturated solution; starting a nanofiltration device to carry out ammonium removal treatment; concentrating the filtrate, so that the kasugamycin content is 0.5-4%; and sequentially carrying out re-filtrating, color blending and packaging on the obtained product. Compared with original processes, according to the invention, continuous centrifugalization is newly increased for removing undissolved substances left out by plate-frame filtering, thereby saving a process of rinsing by using a plenty of water, shortening the production cycle, and reducing the production cost; according to the invention, a step of ammonium removal by using a nanofiltration membrane is added, so that ammonium salts in desorption liquid can be effectively and quickly removed, and then used for recycling; and kasugamycin is subjected to certain-procedure concentration, so that the concentration efficiency is increased, the product appearance is improved, and the product quality is enhanced.
Description
Technical field
The invention belongs to microbial source biological bactericide field, be specifically related to kasugarnycin aqua and extract preparation method.
Background technology
Kasugarnycin is the metabolite that actinomycetes produce.Inhale antibiotic in belonging to, have treatment and prevention effect concurrently, sterling is white, needle-shaped crystals solid, stablizes at normal temperatures, stable under acidity and neutrallty condition, easily decomposes in the basic conditions.Kasugarnycin is the favourable agents preventing and treating various bacteria and fungal disease, has prevention, treatment, growth adjustment function.It is prevent and treat outside the special antibiotic of rice blast, also to X. c. pv. oryzicola, oranges and tangerines gummosis, rubber is sick, Prospect on Kiwifruit Bacterial Canker, pepper bacterial scab is sick, early blight of celery, the confused rot of Kidney bean, the white helminthosporium leaf spot of water chestnut, cucumber anthracnose, bacterial angular leaf spot, tomato leaf mildew, gray mold, the control efficiency that cabbage black rot, cucumber fusarium axysporum etc. have had.
Tradition kasugarnycin aqua preparation method is: kasugarnycin zymotic fluid → plate-frame filtering → and from friendship → a large amount of water rinse → desorb → concentrated → multiple filter → color matching → packaging.But owing to containing a large amount of insoluble mycelium, protein and some large molecular impurities in kasugarnycin zymotic fluid, in actual production process, although plate-frame filtering eliminates most large granular impurity, but still there is much granule impurity to need repeatedly to repeat plate-frame filtering, time cycle is long, still have a small amount of impurity to be easily adsorbed on resin, need the water of several times volume to clean the resin adsorbed, produce a large amount of waste water; Meanwhile, filtrate directly concentrates, and steam consumption is large, and production cost is high, the cycle is long, and deposits problems such as easily there being impurity precipitation after there is finished product mixing.
Summary of the invention
The present invention overcomes the deficiencies in the prior art, technical problem to be solved is that the present invention is on original Process ba-sis, improve kasugarnycin zymotic fluid in the pretreatment before friendship, effectively decrease insoluble impurities, eliminate the process with water rinse resin post, greatly reduce the consumption of water, improve from friendship effect; Increase NF membrane simultaneously and take off ammonium operation, in reduction concentrated cost and while the cycle, improve product color, improve product quality.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is: extension process flow process of the present invention: kasugarnycin zymotic fluid → plate-frame filtering → continuous centrifugal → take off ammonium → concentrated → multiple filter → color matching → packaging from friendship → desorb → NF membrane.The present invention improves original extraction work technique, adds continuous centrifugal and NF membrane takes off ammonium two-step, extracts kasugarnycin.
A kind of kasugarnycin aqua extraction process, comprises the following steps: (1) streptomyces microaureus zymotic fluid plate-frame filtering removing mycelia; (2) filtrate continuous centrifugal, removing Small molecular insoluble matter; (3) enter from friendship post from friendship, absorption kasugarnycin molecule also removes other soluble impurities; (4) desorb at normal temperatures of ammonium chloride saturated solution is used; (5) start sodium filter device, carry out de-ammonium process; (6) concentrated filtrate makes kasugarnycin content between 0.5% ~ 4%; (7) multiple filter, color matching, packaging is completed.
In described step (2), the number of times of continuous centrifugal is centrifugal rotating speed is 1200 r/min.
In described step (5), NF membrane takes off the operating pressure 1.5-2.5MPa of ammonium, temperature 25-35 DEG C, de-ammonium process 30-40min.
Compared with prior art the present invention has following beneficial effect.
Compared with former technique, the present invention increases continuous centrifugal removing insoluble matter that plate-frame filtering is omitted newly, eliminates the process with a large amount of water rinse, shortens the production cycle, reduce production cost.
Meanwhile, invention increases NF membrane and take off ammonium step, effectively can slough the ammonium salt in stripping liquid fast, for recycling; The concentrated of certain procedures is carried out to kasugarnycin, has improve thickening efficiency, improve product appearance, improve product quality.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment 1
A kind of kasugarnycin aqua extraction process, comprises the following steps: (1) streptomyces microaureus zymotic fluid plate-frame filtering removing mycelia; (2) filtrate continuous centrifugal, removing Small molecular insoluble matter, the rotating speed of continuous centrifugal is 1200r/min; (3) enter from friendship post from friendship, absorption kasugarnycin molecule also removes other soluble impurities; (4) desorb at normal temperatures of ammonium chloride saturated solution is used; (5) sodium filter device is started, operating pressure 1.5MPa, temperature 25 DEG C, de-ammonium process 30min; (6) concentrated filtrate makes kasugarnycin content between 0.5%; (7) multiple filter, color matching, packaging is completed.
Embodiment 2
A kind of kasugarnycin aqua extraction process, comprises the following steps: (1) streptomyces microaureus zymotic fluid plate-frame filtering removing mycelia; (2) filtrate continuous centrifugal, removing Small molecular insoluble matter, continuous centrifugal rotating speed be 1200r/min; (3) enter from friendship post from friendship, absorption kasugarnycin molecule also removes other soluble impurities; (4) desorb at normal temperatures of ammonium chloride saturated solution is used; (5) sodium filter device is started, operating pressure 2MPa, temperature 28 DEG C, de-ammonium process 35min; (6) concentrated filtrate makes kasugarnycin content between 2%; (7) multiple filter, color matching, packaging is completed.
Embodiment 3
A kind of kasugarnycin aqua extraction process, comprises the following steps: (1) streptomyces microaureus zymotic fluid plate-frame filtering removing mycelia; (2) filtrate continuous centrifugal, removing Small molecular insoluble matter, continuous centrifugal rotating speed is 1200 r/min; (3) enter from friendship post from friendship, absorption kasugarnycin molecule also removes other soluble impurities; (4) desorb at normal temperatures of ammonium chloride saturated solution is used; (5) sodium filter device is started, operating pressure 2MPa, temperature 25 DEG C, de-ammonium process 35min; (6) concentrated filtrate makes kasugarnycin content between 4%; (7) multiple filter, color matching, packaging is completed.
Embodiment 4
A kind of kasugarnycin aqua extraction process, comprises the following steps: (1) streptomyces microaureus zymotic fluid plate-frame filtering removing mycelia; (2) filtrate continuous centrifugal, removing Small molecular insoluble matter, the rotating speed of continuous centrifugal is 1200r/min; (3) enter from friendship post from friendship, absorption kasugarnycin molecule also removes other soluble impurities; (4) desorb at normal temperatures of ammonium chloride saturated solution is used; (5) sodium filter device is started, operating pressure 2.5MPa, temperature 35 DEG C, de-ammonium process 40min; (6) concentrated filtrate makes kasugarnycin content between 3.5%; (7) multiple filter, color matching, packaging is completed.
The present invention can summarize with other the concrete form without prejudice to spirit of the present invention or principal character.Therefore, no matter from which point, above-mentioned embodiment of the present invention all can only be thought explanation of the present invention and can not limit invention, claims indicate scope of the present invention, and scope of the present invention is not pointed out in above-mentioned explanation, therefore, any change in the implication suitable with claims of the present invention and scope, all should think to be included in the scope of claims.
Claims (3)
1. a kasugarnycin aqua extraction process, is characterized in that comprising the following steps: (1) streptomyces microaureus zymotic fluid plate-frame filtering removing mycelia; (2) filtrate continuous centrifugal, removing Small molecular insoluble matter; (3) enter from friendship post from friendship, absorption kasugarnycin molecule also removes other soluble impurities; (4) desorb at normal temperatures of ammonium chloride saturated solution is used; (5) start sodium filter device, carry out de-ammonium process; (6) concentrated filtrate makes kasugarnycin content between 0.5% ~ 4%; (7) multiple filter, color matching, packaging is completed.
2. a kind of kasugarnycin aqua extraction process as claimed in claim 1, is characterized in that the centrifugal rotating speed of continuous centrifugal in described step (2) is 1200r/min.
3. a kind of kasugarnycin aqua extraction process as claimed in claim 1, is characterized in that in described step (5), NF membrane takes off the operating pressure 1.5-2.5MPa of ammonium, temperature 25-35 DEG C, de-ammonium process 30-40min.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510196286.XA CN104770369A (en) | 2015-04-23 | 2015-04-23 | Kasugamycin water-aqua extracting process |
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| CN201510196286.XA CN104770369A (en) | 2015-04-23 | 2015-04-23 | Kasugamycin water-aqua extracting process |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106083951A (en) * | 2016-07-01 | 2016-11-09 | 宁夏泰瑞制药股份有限公司 | A kind of method utilizing kasugarnycin broth extraction kasugamycin hydrochloride |
| CN106818752A (en) * | 2016-12-26 | 2017-06-13 | 王震 | A kind of preparation method of high content kasugarnycin aqua |
| CN107987110A (en) * | 2017-12-15 | 2018-05-04 | 陕西麦可罗生物科技有限公司 | A kind of desalination process of kasugarnycin concentrate |
| CN110452275A (en) * | 2019-09-17 | 2019-11-15 | 陕西绿盾环境工程研究院有限公司 | A kind of preparation method of high-purity kasugarnycin |
| CN110743630A (en) * | 2019-11-14 | 2020-02-04 | 江门市植保有限公司 | Ion exchange equipment for preparing kasugamycin aqueous solution and aqueous solution preparation method |
Citations (2)
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|---|---|---|---|---|
| US3358001A (en) * | 1963-12-28 | 1967-12-12 | Umezawa Hamao | Antibiotic kasugamycin |
| CN102250165A (en) * | 2011-06-03 | 2011-11-23 | 陕西绿盾生物制品有限责任公司 | Preparation method of technical grade kasumin |
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2015
- 2015-04-23 CN CN201510196286.XA patent/CN104770369A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3358001A (en) * | 1963-12-28 | 1967-12-12 | Umezawa Hamao | Antibiotic kasugamycin |
| CN102250165A (en) * | 2011-06-03 | 2011-11-23 | 陕西绿盾生物制品有限责任公司 | Preparation method of technical grade kasumin |
Non-Patent Citations (3)
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| 吴梧桐主编: "《生物制药工艺学》", 30 April 2013, 中国医药科技出版社 * |
| 王晓琳: "纳滤膜分离机理及其应用研究进展", 《化学通报》 * |
| 韩长日等主编: "《精细有机化工产品生产技术手册》", 30 June 2010, 中国石化出版社 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106083951A (en) * | 2016-07-01 | 2016-11-09 | 宁夏泰瑞制药股份有限公司 | A kind of method utilizing kasugarnycin broth extraction kasugamycin hydrochloride |
| CN106083951B (en) * | 2016-07-01 | 2018-11-27 | 宁夏泰瑞制药股份有限公司 | A method of utilizing kasugarnycin broth extraction kasugamycini hydrochloride |
| CN106818752A (en) * | 2016-12-26 | 2017-06-13 | 王震 | A kind of preparation method of high content kasugarnycin aqua |
| CN106818752B (en) * | 2016-12-26 | 2019-04-02 | 王震 | A kind of preparation method of high-content kasugarnycin aqua |
| CN107987110A (en) * | 2017-12-15 | 2018-05-04 | 陕西麦可罗生物科技有限公司 | A kind of desalination process of kasugarnycin concentrate |
| CN110452275A (en) * | 2019-09-17 | 2019-11-15 | 陕西绿盾环境工程研究院有限公司 | A kind of preparation method of high-purity kasugarnycin |
| CN110452275B (en) * | 2019-09-17 | 2023-04-07 | 陕西绿盾环境工程研究院有限公司 | Preparation method of high-purity kasugamycin |
| CN110743630A (en) * | 2019-11-14 | 2020-02-04 | 江门市植保有限公司 | Ion exchange equipment for preparing kasugamycin aqueous solution and aqueous solution preparation method |
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