CN104770105A - Method for promoting baical skullcap root seed germination - Google Patents
Method for promoting baical skullcap root seed germination Download PDFInfo
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Abstract
The invention discloses a method for promoting baical skullcap root seed germination. The method comprises the step of pretreating baical skullcap root seeds with a plant growth regulator before seed germination, wherein the plant growth regulator is a dry distillation liquid obtained by carrying out dry distillation on hawthorn fruit seeds, spina date seeds and walnut shells in a mass ratio of (1-13) to (1-2) to (1-3). The method has the beneficial effects that the baical skullcap root seeds are pretreated by adopting the plant dry distillation liquid as the plant growth regulator; the pretreatment method is simple, is easy to operate, is pollution-free, can conduce to obviously increasing the germination rates of the baical skullcap root seeds and has high economic benefits; the used plant dry distillation liquid is obtained by carrying out dry distillation on the hawthorn fruit seeds, the walnut shells and the spina date seeds; the preparation method of the plant dry distillation liquid is simple to operate, is concise and fast and is easy to achieve; the used raw materials are plant wastes, so that the method has the effects of achieving full utilization of the wastes and turning wastes into wealth, is low in cost and is convenient for achieving industrial production.
Description
Technical field
The present invention relates to a kind of method promoting root of large-flowered skullcap seed germination, be specifically related to dry distillation liquid that a kind of utilization extracts from plant to promote the method in root of large-flowered skullcap seed germination, belong to seed processing technology field.
Background technology
Along with being filled with of " back to nature " trend, traditional natural plant is more and more subject to the favor of people.The root of large-flowered skullcap derives from the dry root of labiate root of large-flowered skullcap Scutellaria baicalensis Georgi, and having heat-clearing and damp-drying drug, purging intense heat and detonicating, hemostasis, the effect such as antiabortive, is that China commonly uses one of large traditional Chinese medicine.The demand of current domestic and international market to radix scutellariae medicinal materials increases day by day, and production scale and cultivated area constantly expand.Main based on seminal propagation in production, seed germination speed and emergence rate directly affect the growth in medicinal material later stage.Root of large-flowered skullcap seed grain is little, skin depth, hard, causes germination rate not high, seriously governs the raising of producing upper root of large-flowered skullcap output.For improving seed germination rate, in production, the growth regulators such as gibberellin that adopt carry out seed germination pre-treatment more, gibberellin is Prof. Du Yucang, there is the problems such as cost is high, seriously polluted, therefore finds and effectively promotes that the approach of root of large-flowered skullcap seed germination is more and more important for the output improving radix scutellariae medicinal materials.
Dry distillation liquid refers to the concentrated liquid being put in retort by vegetable material and heating the natural cooling liquid of the flue gas of emerging in carbonization process and obtain.In recent years, the medical and edibility of dry distillation liquid comes into one's own, and is widely used in the fields such as sterilization, prevention from suffering from the diseases, food antiseptic, seasoning, fruit freshness preserving.But, dry distillation liquid is rarely had report on traditional Chinese medicine seed germination.
Summary of the invention
The object of this invention is to provide a kind of method promoting root of large-flowered skullcap seed germination, the method carries out pretreatment using plant dry distillation liquid as plant growth regulator to root of large-flowered skullcap seed, can significantly improve root of large-flowered skullcap seed germination, significant to production upper raising root of large-flowered skullcap output.
At present, dry distillation liquid is used in medicine mainly as sterilization, antipathogenic composition, in promotion traditional Chinese medicine seed germination, have no report.Inventor finds under study for action, and dry distillation liquid can significantly be promoted root of large-flowered skullcap seed germination as plant growth regulator, and from the experimental results, the facilitation of dry distillation liquid to root of large-flowered skullcap seed germination of different plant origin is different.Inventor is through a large amount of screening experiment, finally obtain the dry distillation liquid that effectively can promote root of large-flowered skullcap seed germination, and specifically have studied preparation method and the using method of this dry distillation liquid, thus for dry distillation liquid is used for promoting that root of large-flowered skullcap seed germination provides important technical support.
The concrete technical scheme of the present invention is as follows:
A kind of method promoting root of large-flowered skullcap seed germination, it is characterized in that: before seed germination, with plant growth regulator, pretreatment is carried out to root of large-flowered skullcap seed, described plant growth regulator is is the Chinese hawthorn seed of 1 ~ 13:1 ~ 2:1 ~ 3 by mass ratio, jujube nucleus and walnut shell carry out the dry distillation liquid that destructive distillation obtains, and this dry distillation liquid also can be called plant dry distillation liquid.
The present invention's plant growth regulator used is a Plants dry distillation liquid, and it is preferably, and mass ratio is the Chinese hawthorn seed of 11:2:2 or 13:1:1, jujube nucleus and walnut shell carry out the dry distillation liquid that destructive distillation obtains.
The present invention's plant growth regulator used, prepare in accordance with the following methods: Chinese hawthorn seed, jujube nucleus and walnut shell are mixed by proportioning, destructive distillation is carried out respectively at 140 ~ 145 DEG C, 240 ~ 245 DEG C, 300 ~ 360 DEG C, merge the dry distillation liquid of gained at these three sections of temperature, by the dry distillation liquid removal of impurities after merging, obtain plant growth regulator.
When preparing plant growth regulator, at 140 ~ 145 DEG C of insulation 1-2h, at 240 ~ 245 DEG C of insulation 2-3 hour, at 300 ~ 360 DEG C of insulation 1-2 hour.
When preparing plant growth regulator, the dry distillation liquid after merging adopts following method removal of impurities: by merge after dry distillation liquid stir, stratification, get supernatant; Distilled by supernatant, filter after gained distillate charcoal treatment, gained filtrate is plant dry distillation liquid.
During dry distillation liquid removal of impurities, supernatant is 100 ~ 105 DEG C of air-distillations.
During dry distillation liquid removal of impurities, supernatant distillation time is 2h.
During dry distillation liquid removal of impurities, distillate adds the active carbon of its quality 10%.
During dry distillation liquid removal of impurities, active carbon processes 45 minutes at 95 DEG C, carries out removal of impurities.
The present invention promotes in the method for root of large-flowered skullcap seed germination, carrying out pretreated process with plant growth regulator to seed is: mixed with the volume ratio of water according to 1:500-2000 by plant growth regulator, obtain preprocessing solution, preprocessing solution is sprayed at root of large-flowered skullcap the surface of the seed, pretreatment is carried out to root of large-flowered skullcap seed.
The present invention promotes that, in the method for root of large-flowered skullcap seed germination, the volume ratio of plant growth regulator and water is preferably 1:1000.
The present invention promotes in the method for root of large-flowered skullcap seed germination, and during root of large-flowered skullcap Seeds preprocess, the every 4h of preprocessing solution sprays once, sprays 48h continuously.
The present invention promotes in the method for root of large-flowered skullcap seed germination, during root of large-flowered skullcap Seeds preprocess, sprays preprocessing solution and is advisable to drench seed coat.
The present invention promotes in the method for root of large-flowered skullcap seed germination, selects the root of large-flowered skullcap seed of full seed health to sprout.
The present invention promotes, in the method for root of large-flowered skullcap seed germination, first to be carried out disinfection with liquor natrii hypochloritis by root of large-flowered skullcap seed, then rinses well with water, then carries out pretreatment with plant growth regulator to root of large-flowered skullcap seed.Liquor natrii hypochloritis's concentration used can be 0.525gL
-1, sterilization about 10min.
After carrying out pretreatment to seed according to the method described above, damp sand seed being placed in water content 60% is sprouted, temperature: 25 DEG C/20 DEG C (daytime/night); Illumination: 14h light/10h is dark, and light intensity is 260-270 μm of ol/ (m
2s); Relative moisture: 65-70%.After tested, through pretreated root of large-flowered skullcap seed than not significantly improving through pretreated germination rate.
The present invention adopts plant dry distillation liquid to carry out pretreatment as plant growth regulator to root of large-flowered skullcap seed, and preprocess method is simple, is easy to operation, pollution-free, can significantly improve the germination rate of root of large-flowered skullcap seed, high financial profit.And plant dry distillation liquid used be Chinese hawthorn seed, walnut shell, the core of this three Plants of jujube nucleus, shell carry out the dry distillation liquid that destructive distillation obtains, its preparation method is simple to operate, fast succinct, be easy to realize, raw materials used is plant refuse, achieves making full use of of refuse, turns waste into wealth, cost is low, is convenient to suitability for industrialized production.
Embodiment
Below by specific embodiment, the present invention will be further elaborated, it is to be understood that following explanation is only to explain the present invention, do not limit its content.
The preparation of plant growth regulator
Embodiment 1
Get Chinese hawthorn seed, jujube nucleus and walnut shell 1.5kg respectively, be that 1:1:1 adds retort, be heated to 140 ~ 145 DEG C with mass ratio, be incubated 1 hour, continue to be heated to 240 ~ 245 DEG C, be incubated 2 hours, then temperature rises to 300 ~ 360 DEG C and maintains 1 hour.Collect the dry distillation liquid of day part, mix and blend 30 minutes, proceed in separatory funnel and leave standstill 12 hours, separate supernatant.Supernatant is added distillation still, in 100 ~ 105 DEG C of distillations 2 hours, collects distillate.Distillate adds the active carbon of 10wt%, is heated with stirring to 95 DEG C, maintains 45 minutes, filters, and collects filtered fluid.Filtrate is plant growth regulator of the present invention, also can be called plant dry distillation liquid, lower same.
Embodiment 2
Get Chinese hawthorn seed, jujube nucleus and walnut shell and amount to 1.5kg, mass ratio is that 6:2:3 adds retort, is heated to 140 ~ 145 DEG C, is incubated 1 hour, continues to be heated to 240 ~ 245 DEG C, is incubated 2 hours, and then temperature rises to 300 ~ 360 DEG C and maintains 1 hour.Collect the dry distillation liquid of day part, mix and blend 30 minutes, proceed in separatory funnel and leave standstill 12 hours, separate supernatant.Supernatant is added distillation still, in 100 ~ 105 DEG C of distillations 2 hours, collects distillate.Distillate adds the active carbon of 10wt%, is heated with stirring to 95 DEG C, maintains 45 minutes, filters, and collects filtered fluid.Filtrate is plant growth regulator of the present invention.
Embodiment 3
Get Chinese hawthorn seed, jujube nucleus and walnut shell and amount to 1.5kg, mass ratio is that 11:2:2 adds retort, is heated to 140 ~ 145 DEG C, is incubated 1 hour, continues to be heated to 240 ~ 245 DEG C, is incubated 2 hours, and then temperature rises to 300 ~ 360 DEG C and maintains 1 hour.Collect the dry distillation liquid of day part, mix and blend 30 minutes, proceed in separatory funnel and leave standstill 12 hours, separate supernatant.Supernatant is added distillation still, in 100 ~ 105 DEG C of distillations 2 hours, collects distillate.Distillate adds the active carbon of 10wt%, is heated with stirring to 95 DEG C, maintains 45 minutes, filters, and collects filtered fluid.Filtrate is plant growth regulator of the present invention.
Embodiment 4
Get Chinese hawthorn seed, jujube nucleus and walnut shell and amount to 1.5kg, mass ratio is that 13:1:1 adds retort, is heated to 140 ~ 145 DEG C, is incubated 1 hour, continues to be heated to 240 ~ 245 DEG C, is incubated 2 hours, and then temperature rises to 300 ~ 360 DEG C and maintains 1 hour.Collect the dry distillation liquid of day part, mix and blend 30 minutes, proceed in separatory funnel and leave standstill 12 hours, separate supernatant.Supernatant is added distillation still, in 100 ~ 105 DEG C of distillations 2 hours, collects distillate.Distillate adds the active carbon of 10wt%, is heated with stirring to 95 DEG C, maintains 45 minutes, filters, and collects filtered fluid.Filtrate is plant growth regulator of the present invention.
Comparative example 1
Plant growth regulator is prepared according to the method for embodiment 3, unlike, plant resource used is: the trunk of oak (Magnoliadenudata), Chinese scholar tree (Sophora japonica), yellow rose (Rosa xanthina Lindl) this three Plants, their mass ratio is 1:1:1.
Comparative example 2
Plant growth regulator is prepared according to the method for embodiment 3, unlike, plant resource used is: the trunk of cercis (Cercischinensin Bunge), weeping willow (Salix babylonica), chinaberry (Melia azedarach) this three Plants, their mass ratio is 1:1:1.
The application examples of plant growth regulator treatment root of large-flowered skullcap seed
1, choose the root of large-flowered skullcap seed of full seed health, use 0.525gL
-1hypochlorite disinfectant 10min, then uses distilled water flushing 3 times.
2, preprocessing solution is prepared: plant growth regulator above-described embodiment and comparative example prepared individually mixes with the volume ratio of water according to 1:500,1:1000 and 1:2000, is made into different preprocessing solutions.
3, the pretreatment of root of large-flowered skullcap seed: the preprocessing solution of preparation is sprayed on respectively the root of large-flowered skullcap the surface of the seed disinfected, be advisable to drench seed coat, every 4h sprays once, uninterrupted sprinkling in 48h, carry out adopting pure water to carry out the blank sprayed, each process 6 repetition simultaneously.
4, seed germination: the damp sand that pretreated root of large-flowered skullcap seed is placed in water content 60% is sprouted, temperature: 25 DEG C/20 DEG C (daytime/night); Illumination: 14h light/10h is dark, and light intensity is 260-270 μm of ol/ (m
2s); Relative moisture: 65-70%.Record the moisture keeping the skin wet in time in seed germination quantity (breaking through seed coat with radicle to be as the criterion) germination process to guarantee required for seed germination every day.25d terminates to sprout experiment, and measure all seedling root length alive, fresh weight, statistics germination rate, measures amylase activity (enzymic activity is higher, and germination rate is higher).The mensuration of amylase activity: amylase activity adopts ultraviolet method, represents diastatic relative activity with OD value size variation per minute.
It is as follows that each group of root of large-flowered skullcap seed cultivates each index after 25 days according to the method described above:
Table 1 is group root of large-flowered skullcap seed germination rate and growth of seedling situation respectively
As can be seen from the data of above-mentioned table 1, the plant growth regulator of the embodiment of the present invention 1,2,3,4 all can the germination rate of raising root of large-flowered skullcap seed in various degree, seed starches enzymic activity, fresh weight and radicle length compared with blank, and can be found out by the contrast between them, the facilitation of embodiment 3 and embodiment 4 is comparatively remarkable, and embodiment 1,2 is taken second place.Can be found out by the contrast of plant growth regulator of the present invention and comparative example plant growth regulator, the present invention's plant growth regulator used can significantly improve the germination rate of root of large-flowered skullcap seed, seed starches enzymic activity, fresh weight and radicle length.
Claims (10)
1. one kind promotes the method for root of large-flowered skullcap seed germination, it is characterized in that: before seed germination, with plant growth regulator, pretreatment is carried out to root of large-flowered skullcap seed, described plant growth regulator is is the Chinese hawthorn seed of 1 ~ 13:1 ~ 2:1 ~ 3 by mass ratio, jujube nucleus and walnut shell carry out the dry distillation liquid that destructive distillation obtains.
2. method according to claim 1, is characterized in that: mass ratio 11:2:2 or 13:1:1 of Chinese hawthorn seed, jujube nucleus and walnut shell.
3. method according to claim 1 and 2, it is characterized in that: carrying out pretreated process with plant growth regulator to seed is: mixed with the volume ratio of water according to 1:500-2000 by plant growth regulator, obtain preprocessing solution, preprocessing solution is sprayed at root of large-flowered skullcap the surface of the seed, pretreatment is carried out to root of large-flowered skullcap seed.
4. method according to claim 3, is characterized in that: during root of large-flowered skullcap Seeds preprocess, and the every 4h of preprocessing solution sprays once, sprays 48h continuously.
5. method according to claim 3, is characterized in that: during root of large-flowered skullcap Seeds preprocess, sprays preprocessing solution and is advisable to drench seed coat.
6. the method according to claim 1,2 or 3, is characterized in that: before carrying out pretreatment with plant growth regulator to root of large-flowered skullcap seed, first carried out disinfection with liquor natrii hypochloritis by root of large-flowered skullcap seed, then rinse well with water.
7. the method according to claim 1,2 or 3, it is characterized in that: the preparation method of plant growth regulator is: Chinese hawthorn seed, jujube nucleus and walnut shell are mixed by proportioning, destructive distillation is carried out respectively at 140 ~ 145 DEG C, 240 ~ 245 DEG C, 300 ~ 360 DEG C, merge the dry distillation liquid of gained at these three sections of temperature, by the dry distillation liquid removal of impurities after merging, obtain plant growth regulator.
8. method according to claim 7, is characterized in that: when preparing plant growth regulator, at 140 ~ 145 DEG C of insulation 1-2h, at 240 ~ 245 DEG C of insulation 2-3 hour, at 300 ~ 360 DEG C of insulation 1-2 hour.
9. method according to claim 7, is characterized in that: when preparing plant growth regulator, and the dry distillation liquid after merging adopts following method removal of impurities: by merge after dry distillation liquid stir, stratification, get supernatant; Distilled by supernatant, filter after gained distillate charcoal treatment, gained filtrate is plant growth regulator.
10. method according to claim 9, is characterized in that: when preparing plant growth regulator, and supernatant is at 100 ~ 105 DEG C of air-distillation 2h; The active carbon that distillate adds its quality 10% processes 45 minutes at 95 DEG C.
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| CN106233868A (en) * | 2016-07-29 | 2016-12-21 | 北京市农业技术推广站 | A kind of initiating method improving Radix Scutellariae seed germination rate and Seedling Quality |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106233868A (en) * | 2016-07-29 | 2016-12-21 | 北京市农业技术推广站 | A kind of initiating method improving Radix Scutellariae seed germination rate and Seedling Quality |
| CN106233868B (en) * | 2016-07-29 | 2019-12-03 | 北京市农业技术推广站 | A kind of initiation method improving radix scutellariae seed germination rate and Seedling Quality |
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