CN104755933B - 用于鉴定hiv中和抗体的方法 - Google Patents

用于鉴定hiv中和抗体的方法 Download PDF

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Publication number
CN104755933B
CN104755933B CN201380052377.5A CN201380052377A CN104755933B CN 104755933 B CN104755933 B CN 104755933B CN 201380052377 A CN201380052377 A CN 201380052377A CN 104755933 B CN104755933 B CN 104755933B
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China
Prior art keywords
antibody
cells
binding
hiv
fusion protein
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CN201380052377.5A
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Chinese (zh)
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CN104755933A (zh
Inventor
朱利安·米格尔·布兰科阿韦斯
约根·卡里洛莫利纳
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IrsiCaixa Institut de Recerca de la Sida
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IrsiCaixa Institut de Recerca de la Sida
Laboratorios del Dr Esteve SA
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G01N33/56988—HIV or HTLV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—RNA viruses
    • C07K16/112—Retroviridae (F), e.g. leukemia viruses
    • C07K16/114—Lentivirus (G), e.g. human immunodeficiency virus [HIV], feline immunodeficiency virus [FIV] or simian immunodeficiency virus [SIV]
    • C07K16/1145—Env proteins, e.g. gp41, gp110/120, gp160, V3, principal neutralising domain [PND] or CD4-binding site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/34—Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
    • C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
    • G01N2333/155—Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
    • G01N2333/16—HIV-1, HIV-2
    • G01N2333/162—HIV-1, HIV-2 env, e.g. gp160, gp110/120, gp41, V3, peptid T, DC4-Binding site

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • AIDS & HIV (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oncology (AREA)
CN201380052377.5A 2012-09-06 2013-09-06 用于鉴定hiv中和抗体的方法 Expired - Fee Related CN104755933B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12382342.9 2012-09-06
EP12382342.9A EP2706356A1 (en) 2012-09-06 2012-09-06 Methods for identifying HIV neutralizing antibodies
PCT/EP2013/068446 WO2014037490A1 (en) 2012-09-06 2013-09-06 Methods for identifying hiv neutralizing antibodies

Publications (2)

Publication Number Publication Date
CN104755933A CN104755933A (zh) 2015-07-01
CN104755933B true CN104755933B (zh) 2017-06-09

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CN201380052377.5A Expired - Fee Related CN104755933B (zh) 2012-09-06 2013-09-06 用于鉴定hiv中和抗体的方法

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US (1) US9952218B2 (https=)
EP (2) EP2706356A1 (https=)
JP (1) JP6282655B2 (https=)
KR (1) KR20150081255A (https=)
CN (1) CN104755933B (https=)
AU (1) AU2013311626A1 (https=)
BR (1) BR112015004970A2 (https=)
CA (1) CA2884165A1 (https=)
ES (1) ES2643268T3 (https=)
IL (1) IL237613A0 (https=)
IN (1) IN2015DN02730A (https=)
MX (1) MX2015003047A (https=)
PT (1) PT2893349T (https=)
RU (1) RU2015112297A (https=)
WO (1) WO2014037490A1 (https=)
ZA (1) ZA201502210B (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106166313B (zh) * 2016-07-01 2018-09-07 浙江大学 一种艾滋病孕妇血液净化器
CN106075626B (zh) * 2016-07-01 2018-06-26 浙江大学 一种艾滋病血液净化治疗仪

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003040311A2 (en) * 2001-10-25 2003-05-15 The Government Of The United States Of America As Represented By The Secretary Of Health And Human Services Efficient inhibition of hiv-1 viral entry through a novel fusion protein including of cd4
CN1668633A (zh) * 2002-07-23 2005-09-14 杜克大学 IgG Fc/HIV－gp120/C3d 融合蛋白质
WO2005121175A2 (en) * 2004-06-08 2005-12-22 Novartis Vaccines And Diagnostics Inc. Env polypeptide complexes and methods of use
US20070243208A1 (en) * 1999-03-16 2007-10-18 The Govt. Of The U.S.A As Represented By The Secretary Of The Dept. Of Health & Human Services Novel chimeric protein for prevention and treatment of hiv infection
CN101146827A (zh) * 2005-02-03 2008-03-19 诺华疫苗和诊断公司 Hiv tat-cd4杂合分子及其使用方法
CN101551392A (zh) * 2008-01-22 2009-10-07 厦门大学 一种检测人乳头瘤病毒中和抗体的方法
CN101846679A (zh) * 2009-03-25 2010-09-29 邢安辉 筛选含有外源融合基因HIV/env/IFNα-2b的重组痘苗病毒的方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5091513A (en) 1987-05-21 1992-02-25 Creative Biomolecules, Inc. Biosynthetic antibody binding sites
EP3957651A1 (en) * 2010-05-21 2022-02-23 The United States of America, as Represented By the Secretary, Department of Health and Human Services High-affinity fully functional soluble single-domain human cd4, antibodies, and related fusion proteins

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070243208A1 (en) * 1999-03-16 2007-10-18 The Govt. Of The U.S.A As Represented By The Secretary Of The Dept. Of Health & Human Services Novel chimeric protein for prevention and treatment of hiv infection
WO2003040311A2 (en) * 2001-10-25 2003-05-15 The Government Of The United States Of America As Represented By The Secretary Of Health And Human Services Efficient inhibition of hiv-1 viral entry through a novel fusion protein including of cd4
CN1668633A (zh) * 2002-07-23 2005-09-14 杜克大学 IgG Fc/HIV－gp120/C3d 融合蛋白质
WO2005121175A2 (en) * 2004-06-08 2005-12-22 Novartis Vaccines And Diagnostics Inc. Env polypeptide complexes and methods of use
CN101146827A (zh) * 2005-02-03 2008-03-19 诺华疫苗和诊断公司 Hiv tat-cd4杂合分子及其使用方法
CN101551392A (zh) * 2008-01-22 2009-10-07 厦门大学 一种检测人乳头瘤病毒中和抗体的方法
CN101846679A (zh) * 2009-03-25 2010-09-29 邢安辉 筛选含有外源融合基因HIV/env/IFNα-2b的重组痘苗病毒的方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Bifunctional fusion proteins of the human enguneered antibody domain m36 with human solbule CD4 are potent inhibitors of diverse HIV-1 isolates;Chen W et al;《Antiviral Research》;20101001;第88卷(第1期);107-115 *

Also Published As

Publication number Publication date
PT2893349T (pt) 2018-04-12
WO2014037490A1 (en) 2014-03-13
IN2015DN02730A (https=) 2015-09-04
MX2015003047A (es) 2015-07-14
AU2013311626A1 (en) 2015-04-30
KR20150081255A (ko) 2015-07-13
CA2884165A1 (en) 2014-03-13
EP2706356A1 (en) 2014-03-12
EP2893349B1 (en) 2017-08-16
CN104755933A (zh) 2015-07-01
EP2893349A1 (en) 2015-07-15
JP6282655B2 (ja) 2018-02-21
ZA201502210B (en) 2016-11-30
JP2015530575A (ja) 2015-10-15
US9952218B2 (en) 2018-04-24
RU2015112297A (ru) 2016-10-27
ES2643268T3 (es) 2017-11-21
US20150233924A1 (en) 2015-08-20
IL237613A0 (en) 2015-04-30
HK1211694A1 (en) 2016-05-27
BR112015004970A2 (pt) 2017-07-04

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Address after: Badalona, Spain

Patentee after: Fundacio Privada Institut de Recerca de La Sida-Caixa

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