CN104745465A - Quick uniform heating die plate for biochemical detection - Google Patents
Quick uniform heating die plate for biochemical detection Download PDFInfo
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- CN104745465A CN104745465A CN201510114174.5A CN201510114174A CN104745465A CN 104745465 A CN104745465 A CN 104745465A CN 201510114174 A CN201510114174 A CN 201510114174A CN 104745465 A CN104745465 A CN 104745465A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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Abstract
The invention provides a quick uniform heating die plate for biochemical detection. The quick uniform heating die plate comprises more than one base plate; more than one agent cavity is formed in each base plate; the base plates are connected in parallel and manufactured from conductive and heat transfer materials; the agent cavities are directly heated through the joule heat of the base plates; open holes are formed in two sides or the middle parts of the base plates; two sides of each base plate are divided into more than two current ports; an area can be uniformly heated by adjusting. According to the quick uniform heating die plate, the heat transfer link can be reduced, the heating efficiency is increased, and necessary foundation is provided for quick temperature control.
Description
Technical field
The present invention relates to biochemical pharmaceutical experiment instrument field, be specifically related to a kind of rapid and uniform heating template for biochemistry detection.
Background technology
The amplification procedure of polymerase chain reaction PCR refers to and the archaeal dna polymerase in micro-biological sample is carried out specific gene amplification according to the feature of DNA profiling under the effect of catalyzer.Following three parts will be comprised: high-temperature denatured, low-temperature annealing and middle temperature primer extension in whole process.Sample generally needs the working cycle of 20-40 time, thus realizes the amplification of nucleic acid.Ring mediated isothermal nucleic acid augmentative instrument needs reagent to be heated to specified temp equally, realizes the amplification of nucleic acid.So the two all needs fast and uniform heating means.
As everyone knows, the amount of reagent and the size of well heater, will directly have influence on the effect of heating and heat radiation.In existing PCR Thermal Cycling, heat-processed often becomes the determinative of restriction its of length cycling time.Such as: Peltier heating of today.Because it is subject to the restriction of conducting self-heating principle, heating efficiency often can not realize the object of rapid heating.Now powerful joule formula heating, due in its design in order to homogeneous heating, multiple resistance wire is posted on below thick heat conduction template.Utilize the heat of resistance wire to pass to heat conduction template, and then the heat of heat conduction template is passed to reagent chamber, heating efficiency also can not be too high by the restriction of its structure.
Summary of the invention
In order to overcome the shortcoming of above-mentioned prior art, the object of the present invention is to provide a kind of rapid and uniform heating template for biochemistry detection, reduce heat transfer link, heating efficiency is high, realizes real Rapid nucleic acid amplification procedure.
In order to achieve the above object, the technical scheme that the present invention takes is:
A kind of rapid and uniform heating template for biochemistry detection, comprise more than one backing material plate 1, each backing material plate 1 is provided with more than one reagent chamber 2, parallel way is adopted to connect between backing material plate 1, backing material plate 1 adopts conduction and the material of heat conduction is made, directly utilizes the joule heating of backing material plate 1 to heat reagent chamber 2.
Both sides or the middle part of described backing material plate 1 are provided with perforate 3.
The both sides of described backing material plate 1 are divided into plural electric current port, by regulating feasible region homogeneous heating.
Described reagent chamber 2 comprises more than one reagent react groove or reactor.
Described backing material plate 1 adopts aluminium alloy or Cu alloy material, and the thickness of backing material plate 1 is 0.01-2 millimeter.
Advantage of the present invention: quantitative according to joule, Q=I
2× R × t.Flow through conductor by square to be directly proportional according to its resistance, and resistance value R is directly proportional, and the time t flow through is directly proportional.Even if due to backing material plate 1 do very thin, its resistance is also still less, so cannot normally use with common power, the present invention proposes to increase electric current to ensure heat, existing backing material plate 1 direct heating can be utilized again, the present invention reduces heat transfer link, rapid and uniform heating, realizes real Rapid nucleic acid amplification procedure.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of the embodiment of the present invention 1 without the backing material plate 1 of perforate, central whole formula Joule heating.
Fig. 2 is the schematic diagram of backing material plate 1 that there are perforate, central whole formula Joule heating in the embodiment of the present invention 2 both sides.
Fig. 3 is that the embodiment of the present invention 3 both sides have perforate but the schematic diagram of the backing material plate 1 of monoblock type Joule heating that narrows of center.
Fig. 4 has perforate in the middle of the embodiment of the present invention 4, center separately, the schematic diagram of each heating circuit independently backing material plate 1 of Joule heating.
Fig. 5 is in the middle of the embodiment of the present invention 5 and all there is perforate both sides, and center separates, the schematic diagram of each heating circuit independently backing material plate 1 of Joule heating.
Fig. 6 be the embodiment of the present invention 6 without perforate, center separately, the schematic diagram of each heating circuit independently backing material plate 1 of Joule heating.
Embodiment
Below in conjunction with drawings and Examples, the present invention is described in detail.
Embodiment 1, with reference to Fig. 1, a kind of rapid and uniform heating template for biochemistry detection, comprise a backing material plate 1, backing material plate 1 is provided with a reagent chamber 2, and backing material plate 1 adopts aluminum alloy materials made, directly utilizes the joule heating of backing material plate 1 to heat reagent chamber 2, the both sides of backing material plate 1 are divided into 4 electric current ports, by regulating feasible region homogeneous heating.
The heating initial stage, uniformity electric current is (namely Xi to Yi is as shown in straight arrows A) from left to right, if the lower transverse electric potential difference that can increase of temperature of every primary Ioops is to improve temperature in four transverse loops, if longitudinal temperature is uneven, adjusted (its loop is as shown in curve arrow B in figure) by the electromotive force between the electromotive force between port x i or Yi.In addition the present embodiment also comprises the situation that two ends port is not limited to four, can be n port of similar structures; Xi to Yj, or between Xi, or between Yi, the various heating circuits of (1≤i, j≤n) combine thus reach the heats more had; Reagent chamber 2 can be square, and can be also the shapes such as circle, trilateral, the reagent trough number in reagent chamber 2 can be arbitrary combination.
Embodiment 2, with reference to Fig. 2, a kind of rapid and uniform heating template for biochemistry detection, comprise a backing material plate 1, backing material plate 1 is provided with a reagent chamber 2, and backing material plate 1 adopts aluminum alloy materials made, directly utilize the joule heating of backing material plate 1 to heat reagent chamber 2, the both sides of backing material plate 1 are provided with perforate 3, and the both sides of backing material plate 1 are divided into four electric current ports, by regulating feasible region homogeneous heating.
Increase hole 3 at both sides port to design, can more reasonably change in the middle of backing material plate 1 and the temperature distribution of both sides, because the resistance value that perforate 3 will change herein, calorific value herein will be changed according to Joule's law, in addition the present embodiment also comprises the situation that two ends port is not limited to four, can be n port of similar structures; Xi to Yj, or between Xi, or between Yj, the various heating circuits of (1≤i, j≤n) combine thus reach the heats more had; Reagent chamber 2 can be square can be also the shapes such as circle, trilateral, and the reagent trough number in reagent chamber 2 can be arbitrary combination.In figure, the perforate 3 of both sides also can be diamond hole, slotted eye or one or more circular ports.
Embodiment 3, with reference to Fig. 3, for a rapid and uniform heating template for biochemistry detection, comprise a backing material plate 1, backing material plate 1 is provided with a reagent chamber 2, backing material plate 1 adopts Cu alloy material made, directly utilize the joule heating of backing material plate 1 to heat reagent chamber 2, the both sides of backing material plate 1 are provided with perforate 3, and the center of backing material plate 1 is narrowed, the both sides of backing material plate 1 are divided into four electric current ports, by regulating feasible region homogeneous heating.
Change the straight line in the middle of backing material plate 1 into fall in the inside linear design, also comprise the curve design fallen in the inside.Because the change of shape is by changing the resistance value size in each loop, especially when changing center into cambered design, by changing the resistance value in centre, the temperature distribution at backing material plate 1 center and both sides will be adjusted further.In addition the present embodiment also comprises the cambered design that middle camber line protrudes laterally; Two ends port is not limited to the situation of four, can be n port of similar structures; Xi to Yj, or between Xi, or between Yj, the various heating circuits of (1≤i, j≤n) combine thus reach the heats more had; Reagent chamber 2 can be square can be also the shapes such as circle, trilateral, and the reagent trough number in reagent chamber 2 can be arbitrary combination.In figure, the perforate 3 of both sides also can be diamond hole, slotted eye or one or more circular ports.
Embodiment 4, with reference to Fig. 4, a kind of rapid and uniform heating template for biochemistry detection, comprise a backing material plate 1, backing material plate 1 is provided with two reagent chamber 2, and backing material plate 1 adopts Cu alloy material made, directly utilize the joule heating of backing material plate 1 to heat reagent chamber 2, the only middle of backing material plate 1 is provided with perforate 3, and the both sides of backing material plate 1 are divided into four electric current ports, by regulating feasible region homogeneous heating.
In figure, each loop of Xi to the Yi of backing material plate 1 is independently, and such benefit is: the temperature distribution that 1, can control centre and both sides.2, be just enough to when the words that the uniformity requirement of the heating in each loop is relatively high can add perforate 3 in centre.And the size of each perforate 3 can control.The point controlled like this will be simpler, but effect is relatively better, and heating is also relatively more even, the present embodiment in addition also comprise each independent loop in the middle of straight line change the cambered design protruding laterally or fall in the inside into; Its two ends port is not limited to the situation of four, can be n port of similar structures; The various heating circuits of Xi to Yi (1≤i≤n) combine thus reach the heats more had; Reagent chamber 2 can be square can be also the shapes such as circle, trilateral, and the reagent trough number in reagent chamber 2 can be arbitrary combination.In figure, perforate 3 also can be diamond hole, rectangular opening or one or more circular ports.
Embodiment 5, with reference to Fig. 5, a kind of rapid and uniform heating template for biochemistry detection, comprise a backing material plate 1, backing material plate 1 is provided with two reagent chamber 2, and backing material plate 1 Cu alloy material is made, directly utilize the joule heating of backing material plate 1 to heat reagent chamber 2, the both sides of backing material plate 1 and centre are provided with perforate 3, and the both sides of backing material plate 1 are divided into four electric current ports, by regulating feasible region homogeneous heating.
In figure, each loop of Xi to Yi is independently, all there is perforate 3 middle and both sides, temperature distribution that is middle and both sides can be controlled more accurately, the size of each perforate 3 can control, the point controlled like this will be more complicated, but effect comparatively method four is better, heats also relative manner four more even.In addition the present embodiment also comprises the middle straight line in each independent loop and changes the cambered design protruding laterally or fall in the inside into; Its two ends port is not limited to the situation of four, can be n port of similar structures; The various heating circuits of Xi to Yi (1≤i≤n) combine thus reach the heats more had.Reagent chamber 2 can be square can be also the shapes such as circle, trilateral, and the reagent trough number in reagent chamber 2 can be arbitrary combination, and in figure, perforate 3 also can be diamond hole, rectangular opening or one or more circular ports.
Embodiment 6, with reference to Fig. 6, a kind of rapid and uniform heating template for biochemistry detection, comprise a backing material plate 1, backing material plate 1 is provided with a reagent chamber 2, and backing material plate 1 adopts Cu alloy material made, directly utilizes the joule heating of backing material plate 1 to heat reagent chamber 2, the both sides of backing material plate 1 are divided into four electric current ports, by regulating feasible region homogeneous heating.
Owing to considering that between each transverse loop, its transverse temperature just can be heated by the voltage between adjustment Xi-Yi by (Xi-Yi loop).Longitudinal temperature can not differ too large under same environment, and in addition the present embodiment also comprises the middle straight line in each independent loop and change the cambered design protruding laterally or fall in the inside into; Its two ends port is not limited to the situation of four, can be n port of similar structures; The various heating circuits of Xi to Yi (1≤i≤n) combine thus reach the heats more had; Reagent chamber 2 can be square can be also the shapes such as circle, trilateral, and the reagent trough number in reagent chamber 2 can be arbitrary combination.
To the method for closed-loop control be adopted during use, only needing the backing material plate 1 regional temperature value according to detecting and its difference at the temperature value of the needs in this region, adjusting the voltage between corresponding port, but resistance value is certain, change voltage and will change electric current.Quantitatively will change the temperature value in its region according to joule, eventually pass till adjustment makes it reach the Heating temperature of needs.
Claims (5)
1. the rapid and uniform heating template for biochemistry detection, comprise more than one backing material plate (1), it is characterized in that: each backing material plate (1) is provided with more than one reagent chamber (2), backing material plate adopts parallel way to connect between (1), backing material plate (1) adopts conduction and the material of heat conduction is made, directly utilizes the joule heating of backing material plate (1) to heat reagent chamber 2.
2. a kind of rapid and uniform heating template for biochemistry detection according to claim 1, is characterized in that: both sides or the middle part of described backing material plate (1) are provided with perforate (3).
3. a kind of rapid and uniform heating template for biochemistry detection according to claim 1, is characterized in that: the both sides of described each backing material plate (1) are divided into plural electric current port, by regulating feasible region homogeneous heating.
4. a kind of rapid and uniform heating template for biochemistry detection according to claim 1, is characterized in that: described reagent chamber (2) comprises more than one reagent react groove or reactor.
5. a kind of rapid and uniform heating template for biochemistry detection according to claim 1, is characterized in that: described backing material plate (1) adopts aluminium alloy or Cu alloy material, and the thickness of backing material plate (1) is 0.01-2 millimeter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510114174.5A CN104745465B (en) | 2015-03-16 | 2015-03-16 | A kind of rapid and uniform heating template for biochemistry detection |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510114174.5A CN104745465B (en) | 2015-03-16 | 2015-03-16 | A kind of rapid and uniform heating template for biochemistry detection |
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| CN104745465A true CN104745465A (en) | 2015-07-01 |
| CN104745465B CN104745465B (en) | 2017-06-20 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1123063A (en) * | 1994-01-31 | 1996-05-22 | 日本钨合金株式会社 | Flat PTC heater and resistance value regulating method for the same |
| CN1245449A (en) * | 1996-12-06 | 2000-02-23 | 英国国防部 | Reaction vessels |
| CN1971280A (en) * | 2006-12-08 | 2007-05-30 | 东北大学 | A transparent temperature program controlled biochip |
| CN203546009U (en) * | 2013-11-18 | 2014-04-16 | 苏州东胜兴业科学仪器有限公司 | Polymerase chain reaction plate |
-
2015
- 2015-03-16 CN CN201510114174.5A patent/CN104745465B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1123063A (en) * | 1994-01-31 | 1996-05-22 | 日本钨合金株式会社 | Flat PTC heater and resistance value regulating method for the same |
| CN1245449A (en) * | 1996-12-06 | 2000-02-23 | 英国国防部 | Reaction vessels |
| CN1971280A (en) * | 2006-12-08 | 2007-05-30 | 东北大学 | A transparent temperature program controlled biochip |
| CN203546009U (en) * | 2013-11-18 | 2014-04-16 | 苏州东胜兴业科学仪器有限公司 | Polymerase chain reaction plate |
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| CN104745465B (en) | 2017-06-20 |
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