CN104745465B - A kind of rapid and uniform heating template for biochemistry detection - Google Patents
A kind of rapid and uniform heating template for biochemistry detection Download PDFInfo
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- CN104745465B CN104745465B CN201510114174.5A CN201510114174A CN104745465B CN 104745465 B CN104745465 B CN 104745465B CN 201510114174 A CN201510114174 A CN 201510114174A CN 104745465 B CN104745465 B CN 104745465B
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Abstract
A kind of rapid and uniform heating template for biochemistry detection, including more than one backing material plate, each backing material plate is provided with more than one reagent chamber, connected using parallel way between backing material plate, backing material plate is made using electrically and thermally conductive material, directly reagent chamber is heated using the Joule heat of backing material plate, the both sides or middle part of backing material plate are provided with perforate, the both sides of each backing material plate are divided into more than two electric current ports, realize that region is uniformly heated by adjusting, the present invention reduces heat transfer link, the efficiency of heating surface is high, to realize that quick temperature control improves necessary basis.
Description
Technical field
The present invention relates to biochemical pharmaceutical experiment instrument field, and in particular to a kind of rapid and uniform heating for biochemistry detection
Template.
Background technology
The amplification procedure of polymerase chain reaction PCR refer to by the archaeal dna polymerase in micro-biological sample catalyst work
Specific gene magnification is carried out with the lower feature according to DNA profiling.Will be comprising three below part in whole process：High temperature becomes
Property, process annealing and middle temperature primer extend.One sample generally requires the cyclic process of 20-40 times, so as to realize the expansion of nucleic acid
Increase.Ring mediated isothermal nucleic acid augmentative instrument needs also exist for for reagent being heated to specified temp realizing the amplification of nucleic acid.So the two
It is required for quick and uniform heating means.
It is well known that the size of the amount of reagent and heater, will directly influence the effect of heating and radiating.It is existing
In PCR Thermal Cyclings, heating process often turns into the determinant for restricting its circulation time length.For example：Paar of today
Patch heating.Because it is limited by conducting self-heating principle, the efficiency of heating surface tends not to realize the purpose of quick heating.Now
Powerful joule formula heating, due to, in order to uniformly heat, multiple resistance wires being posted on into thick heat conduction template in its design
Below.Heat conduction template is given using the heat transfer of resistance wire, then again by the heat transfer of heat conduction template to reagent chamber, heating effect
Rate is also limited without too high by its structure.
The content of the invention
In order to overcome the shortcoming of above-mentioned prior art, it is an object of the invention to provide a kind of for the quick of biochemistry detection
Uniform heated die plate, reduces heat transfer link, and the efficiency of heating surface is high, realizes real Rapid nucleic acid amplification procedure.
In order to achieve the above object, the technical scheme taken of the present invention is：
A kind of rapid and uniform heating template for biochemistry detection, including more than one backing material plate 1, each backing material plate 1
More than one reagent chamber 2 is provided with, is connected using parallel way between backing material plate 1, backing material plate 1 uses electrically and thermally conductive material
Material is made, and reagent chamber 2 is directly heated using the Joule heat of backing material plate 1.
The both sides or middle part of described backing material plate 1 are provided with perforate 3.
The both sides of described backing material plate 1 are divided into more than two electric current ports, realize that region is uniformly heated by adjusting.
Described reagent chamber 2 includes more than one reagent reacting groove or reactor.
Described backing material plate 1 uses aluminium alloy or Cu alloy material, and the thickness of backing material plate 1 is 0.01-2 millimeters.
Advantages of the present invention：Quantitative, the Q=I according to joule2×R×t.Flow through conductor by according to its resistance square into just
Than, and resistance value R is directly proportional, and the time t for flowing through is directly proportional.Because backing material plate 1 is even made thin thin, its resistance is remained on
Smaller, so cannot normally be used with common power, the present invention is proposed to increase electric current to ensure heat, and existing base can be utilized again
Material plate 1 is directly heated, and the present invention reduces heat transfer link, and rapid and uniform heating realizes real Rapid nucleic acid amplification procedure.
Brief description of the drawings
Fig. 1 is schematic diagram of the embodiment of the present invention 1 without perforate, the backing material plate 1 of center monoblock type Joule heating.
Fig. 2 is that the both sides of the embodiment of the present invention 2 have perforate, the schematic diagram of the backing material plate 1 of center monoblock type Joule heating.
Fig. 3 is showing for the backing material plate 1 of the monoblock type Joule heating that the both sides of the embodiment of the present invention 3 have that perforate but center narrow
It is intended to.
Fig. 4 is that have perforate in the middle of the embodiment of the present invention 4, and center is separate, each heating circuit independence Joule heating base material
The schematic diagram of plate 1.
Fig. 5 is in the middle of the embodiment of the present invention 5 and there is perforate both sides, and center is separate, and the joule of each heating circuit independence adds
The schematic diagram of the backing material plate 1 of heat.
Fig. 6 be the embodiment of the present invention 6 without perforate, center is separate, the backing material plate 1 of the Joule heating of each heating circuit independence
Schematic diagram.
Specific embodiment
The present invention is described in detail with reference to the accompanying drawings and examples.
Embodiment 1, reference picture 1, a kind of rapid and uniform heating template for biochemistry detection, including a backing material plate 1, base
Material plate 1 is provided with a reagent chamber 2, and backing material plate 1 is made using aluminum alloy materials, is directly added using the Joule heat of backing material plate 1
Thermal agent chamber 2, the both sides of backing material plate 1 are divided into 4 electric current ports, realize that region is uniformly heated by adjusting.
The heating initial stage, uniformity electric current from left to right (i.e. Xi to Yi is as shown in straight arrows A), in four transverse loops
If the temperature of every primary Ioops is relatively low can to increase transverse potential difference to improve temperature, by end if longitudinal temperature is uneven
The potential between potential or Yi between mouthful Xi is adjusted (its loop is as shown in curve arrow B in figure).The present embodiment removes this
Outside be also not limited to the situation of four comprising two ends port, can be n port of similar structures；Between Xi to Yj, or Xi,
Or the various heating circuits of (1≤i, j≤n) are combined so as to reach the heating effect more having between Yi；Reagent chamber 2 can the side of being
The shape such as shape, or circle, triangle, the reagent trough number in reagent chamber 2 can be any combination.
Embodiment 2, reference picture 2, a kind of rapid and uniform heating template for biochemistry detection, including a backing material plate 1, base
Material plate 1 is provided with a reagent chamber 2, and backing material plate 1 is made using aluminum alloy materials, is directly added using the Joule heat of backing material plate 1
Thermal agent chamber 2, the both sides of backing material plate 1 are provided with perforate 3, and the both sides of backing material plate 1 are divided into four electric current ports, are realized by adjusting
Uniformly heat in region.
Hole 3 is increased in both sides port to design, can more reasonably change the Temperature Distribution with both sides in the middle of backing material plate 1, because
For perforate 3 will change resistance value herein, calorific capacity herein will be changed according to Joule's law, in addition the present embodiment also includes
Two ends port is not limited to the situation of four, can be n port of similar structures；Between Xi to Yj, or Xi, or between Yj
The various heating circuits of (1≤i, j≤n) are combined so as to reach the heating effect more having；Reagent chamber 2 can square can also be
The shapes such as circle, triangle, the reagent trough number in reagent chamber 2 can be any combination.The perforate 3 of both sides can also be in figure
Diamond hole, slotted eye or the circular port of one or more.
Embodiment 3, reference picture 3, a kind of rapid and uniform heating template for biochemistry detection, including a backing material plate 1, base
Material plate 1 is provided with a reagent chamber 2, and backing material plate 1 is made using Cu alloy material, is directly added using the Joule heat of backing material plate 1
Thermal agent chamber 2, the both sides of backing material plate 1 are provided with perforate 3, and the center of backing material plate 1 is narrowed, and the both sides of backing material plate 1 are divided into four electric currents
Port, realizes that region is uniformly heated by adjusting.
Straight line in the middle of backing material plate 1 is changed to the linear design fallen in inner side, also the song including being fallen in inner side
Line is designed.Because the change of shape will change the resistance value size in each loop, especially when center is changed into cambered design, will
Change the resistance value in centre, by the Temperature Distribution of further the adjustment center of backing material plate 1 and both sides.The present embodiment is in addition
Also the cambered design for protruding laterally comprising middle camber line；Two ends port is not limited to the situation of four, can be similar structures
N port；The various heating circuits of (1≤i, j≤n) are combined so as to reach more between Xi to Yj, or Xi, or between Yj
Some heating effects；Reagent chamber 2 can be it is square can also be the shapes such as circle, triangle, the reagent trough number in reagent chamber 2
It can be any combination.The perforate 3 of both sides can also be diamond hole, slotted eye or the circular port of one or more in figure.
Embodiment 4, reference picture 4, a kind of rapid and uniform heating template for biochemistry detection, including a backing material plate 1, base
Material plate 1 is provided with two reagent chambers 2, and backing material plate 1 is made using Cu alloy material, is directly added using the Joule heat of backing material plate 1
Thermal agent chamber 2, backing material plate 1 only in the middle of be provided with perforate 3, the both sides of backing material plate 1 are divided into four electric current ports, by adjust come
Realize that region is uniformly heated.
In figure each loop of Xi to the Yi of backing material plate 1 be it is independent, it is such to be advantageous in that：1st, centre can be controlled
With the Temperature Distribution of both sides.2nd, hole 3 can be increased in centre if the uniformity requirement of the heating in each loop is of a relatively high
Just it is enough to.And the size of each perforate 3 can be control.The point controlled since so will be simpler, but effect is relative
Preferably, heating is also relatively more uniform, and in addition the present embodiment is also changed to laterally comprising each independent loop middle straight line
The cambered design for protruding or being fallen in inner side；Its two ends port is not limited to the situation of four, can be n of similar structures
Port；The various heating circuits of Xi to Yi (1≤i≤n) are combined so as to reach the heating effect more having；Reagent chamber 2 can the side of being
Shape can also be the shapes such as circle, triangle, and the reagent trough number in reagent chamber 2 can be any combination.Perforate 3 also may be used in figure
Being diamond hole, rectangular opening or the circular port of one or more.
Embodiment 5, reference picture 5, a kind of rapid and uniform heating template for biochemistry detection, including a backing material plate 1, base
Material plate 1 is provided with two reagent chambers 2, and the Cu alloy material of backing material plate 1 is made, and examination is directly heated using the Joule heat of backing material plate 1
Agent chamber 2, the both sides and centre of backing material plate 1 are provided with perforate 3, and the both sides of backing material plate 1 are divided into four electric current ports, by adjusting come real
Uniformly heat in existing region.
Each loop of Xi to Yi is independent in figure, and there is perforate 3 middle and both sides, can more be accurately controlled
The Temperature Distribution of middle and both sides, the size of each perforate 3 can be control, so since the point of control will be more complicated, but
But compared with method four more preferably, also relative manner four is more uniform for heating for effect.In addition the present embodiment also individually returns comprising each
Road middle straight line is changed to the cambered design for protruding laterally or being fallen in inner side；Its two ends port is not limited to the feelings of four
Condition, can be n port of similar structures；Xi to Yi (1≤i≤n) various heating circuits combination so as to reach more have plus
Thermal effect.Reagent chamber 2 can be it is square can also be the shapes such as circle, triangle, the reagent trough number in reagent chamber 2 can be
Any combination, perforate 3 can also be diamond hole, rectangular opening or the circular port of one or more in figure.
Embodiment 6, reference picture 6, a kind of rapid and uniform heating template for biochemistry detection, including a backing material plate 1, base
Material plate 1 is provided with a reagent chamber 2, and backing material plate 1 is made using Cu alloy material, is directly added using the Joule heat of backing material plate 1
Thermal agent chamber 2, the both sides of backing material plate 1 are divided into four electric current ports, realize that region is uniformly heated by adjusting.
It is contemplated that (Xi-Yi loops) just can be by by adjusting the voltage between Xi-Yi between each transverse loop
Its transverse temperature is heated.Longitudinal temperature will not differ too big under same environment, and in addition the present embodiment also includes each list
Only loop middle straight line is changed to the cambered design for protruding laterally or being fallen in inner side；Its two ends port is not limited to four
Situation, can be n port of similar structures；The various heating circuits of Xi to Yi (1≤i≤n) are combined so as to reach what is more had
Heating effect；Reagent chamber 2 can be it is square can also be the shapes such as circle, triangle, the reagent trough number in reagent chamber 2 can be with
It is any combination.
Will be using the method for closed-loop control when using, it is only necessary to according to the regional temperature value of backing material plate 1 that detects with
The difference of its temperature value the need for this region adjusts the voltage between corresponding port, but resistance value is certain, change voltage
Electric current will be changed.According to the quantitative temperature value that will change its region of joule, the heating temperature for adjusting and reaching needs is eventually passed
Untill degree.
Claims (1)
1. a kind of rapid and uniform heating template for biochemistry detection, including more than one backing material plate (1), it is characterised in that：
Each backing material plate (1) is provided with more than one reagent chamber (2), is connected using parallel way between backing material plate (1), backing material plate
(1) heating circuit independent assortment according to the actual requirements, backing material plate (1) is made using electrically and thermally conductive material, directly using base
The Joule heat that material plate (1) different heating loop produces heats the different zones of reagent chamber (2)；
The both sides or middle part of described backing material plate (1) are provided with perforate (3), and perforate (3) can change in the middle of backing material plate (1) and both sides
Temperature Distribution, and the size of each perforate (3) can control；
The both sides of each described backing material plate (1) are divided into more than two electric current ports, by the selection of different electric current ports
To realize the unrestricted choice in different heating loop；
Described reagent chamber (2) includes more than one reagent reacting groove or reactor；
Described backing material plate (1) uses aluminium alloy or Cu alloy material, and the thickness of backing material plate (1) is 0.01-2 millimeters.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510114174.5A CN104745465B (en) | 2015-03-16 | 2015-03-16 | A kind of rapid and uniform heating template for biochemistry detection |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510114174.5A CN104745465B (en) | 2015-03-16 | 2015-03-16 | A kind of rapid and uniform heating template for biochemistry detection |
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| Publication Number | Publication Date |
|---|---|
| CN104745465A CN104745465A (en) | 2015-07-01 |
| CN104745465B true CN104745465B (en) | 2017-06-20 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510114174.5A Active CN104745465B (en) | 2015-03-16 | 2015-03-16 | A kind of rapid and uniform heating template for biochemistry detection |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1123063A (en) * | 1994-01-31 | 1996-05-22 | 日本钨合金株式会社 | Flat PTC heater and resistance value regulating method for the same |
| CN1971280A (en) * | 2006-12-08 | 2007-05-30 | 东北大学 | A transparent temperature program controlled biochip |
| CN203546009U (en) * | 2013-11-18 | 2014-04-16 | 苏州东胜兴业科学仪器有限公司 | Polymerase chain reaction plate |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9716052D0 (en) * | 1996-12-06 | 1997-10-01 | Secr Defence | Reaction vessels |
-
2015
- 2015-03-16 CN CN201510114174.5A patent/CN104745465B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1123063A (en) * | 1994-01-31 | 1996-05-22 | 日本钨合金株式会社 | Flat PTC heater and resistance value regulating method for the same |
| CN1971280A (en) * | 2006-12-08 | 2007-05-30 | 东北大学 | A transparent temperature program controlled biochip |
| CN203546009U (en) * | 2013-11-18 | 2014-04-16 | 苏州东胜兴业科学仪器有限公司 | Polymerase chain reaction plate |
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| Publication number | Publication date |
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| CN104745465A (en) | 2015-07-01 |
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