CN104740893A - Ultrasonic-assisted accelerated solvent extraction method for vanilla tincture - Google Patents
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- 235000009499 Vanilla fragrans Nutrition 0.000 title claims abstract description 47
- 235000012036 Vanilla tahitensis Nutrition 0.000 title claims abstract description 47
- 244000263375 Vanilla tahitensis Species 0.000 title claims abstract description 46
- 229940098465 tincture Drugs 0.000 title claims abstract description 34
- 238000000899 pressurised-fluid extraction Methods 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title abstract description 20
- 239000002904 solvent Substances 0.000 claims abstract description 21
- 238000000605 extraction Methods 0.000 claims abstract description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000000706 filtrate Substances 0.000 claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 239000000463 material Substances 0.000 claims abstract description 15
- 238000002360 preparation method Methods 0.000 claims abstract description 10
- 239000000284 extract Substances 0.000 claims abstract description 7
- 230000000717 retained effect Effects 0.000 claims description 7
- 238000012545 processing Methods 0.000 claims description 4
- 238000010008 shearing Methods 0.000 claims description 2
- 238000002525 ultrasonication Methods 0.000 claims 1
- 238000001914 filtration Methods 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 238000002604 ultrasonography Methods 0.000 abstract description 2
- 238000001816 cooling Methods 0.000 abstract 1
- 239000000428 dust Substances 0.000 abstract 1
- 238000002156 mixing Methods 0.000 abstract 1
- 238000009210 therapy by ultrasound Methods 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 12
- 238000005516 engineering process Methods 0.000 description 7
- 239000004480 active ingredient Substances 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 238000012546 transfer Methods 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000000638 solvent extraction Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000290333 Vanilla fragrans Species 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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Abstract
Description
技术领域technical field
本发明涉及制备天然植物酊剂的技术领域，特别涉及一种利用超声辅助加速溶剂萃取香荚兰酊剂的方法。The invention relates to the technical field of preparing natural plant tincture, in particular to a method for accelerating solvent extraction of vanilla tincture by means of ultrasound.
背景技术Background technique
香荚兰豆是一种名贵的天然食品香料，因其丰满、浓郁的香气而被广泛地应用于医药、食品等领域。由于目前酊剂制备工艺落后，使得提取过程时间长，而且有效成分收率低，造成资源的严重浪费。因此，新的提取手段不断应用于酊剂的制备中，以提高酊剂的提取效率和品质。Vanilla bean is a precious natural food spice, which is widely used in medicine, food and other fields because of its plump and rich aroma. Due to the backward tincture preparation technology, the extraction process takes a long time and the yield of active ingredients is low, resulting in a serious waste of resources. Therefore, new extraction methods are continuously applied in the preparation of tinctures to improve the extraction efficiency and quality of tinctures.
随着科技的发展，超声波细胞破碎及加速溶剂萃取技术近几年得到广泛应用。超声波细胞破碎技术是利用超高压能量使细胞膜和细胞壁在外力的作用下被破坏，使细胞内有效物质释放出来。操作简单、样品损失量少并能保持细胞活性。加速溶剂萃取技术是在密闭的容器里，通过加温加压的方式进行提取，大大提高了提取效率，减少了溶剂消耗。该方法可以在极短的时间内，使用最低的溶剂用量对样品进行萃取，并且高温高压能够使溶剂更易进入样品基质内，使有效成分更容易扩散到溶剂中。两者方法结合具有操作简便、省时省溶剂、能够最大限度地对有效成分进行提取。With the development of science and technology, ultrasonic cell disruption and accelerated solvent extraction technology have been widely used in recent years. Ultrasonic cell disruption technology uses ultra-high pressure energy to destroy the cell membrane and cell wall under the action of external force, so that the effective substances in the cell are released. The operation is simple, the sample loss is small and the cell viability can be maintained. Accelerated solvent extraction technology extracts in a closed container by heating and pressurizing, which greatly improves the extraction efficiency and reduces solvent consumption. This method can extract the sample with the lowest amount of solvent in a very short time, and the high temperature and high pressure can make the solvent more easily enter the sample matrix, making it easier for the active ingredient to diffuse into the solvent. The combination of the two methods has the advantages of simple operation, saving time and solvent, and being able to extract effective components to the greatest extent.
利用超声波细胞破碎及加速溶剂萃取技术对天然植物酊剂提取，具有广泛的应用前景，在目前的研究中还未见报道。The extraction of natural plant tinctures by ultrasonic cell disruption and accelerated solvent extraction has broad application prospects, but has not been reported in the current research.
发明内容Contents of the invention
本发明所要解决的问题在于针对传统酊剂制备方法的不足，提供一种超声辅助加速溶剂萃取香荚兰酊剂的制备方法。该方法与现有的酊剂制备方法相比，能够大幅缩短生产周期,减少提取溶剂的用量,并极大地提高有效成分的回收率。该方法制备的香荚兰酊剂，品质明显优于常规的酊剂提取法。The problem to be solved by the present invention is to provide a method for preparing vanilla tincture assisted by ultrasonic-assisted accelerated solvent extraction aiming at the deficiencies of traditional tincture preparation methods. Compared with the existing tincture preparation method, the method can greatly shorten the production cycle, reduce the amount of extraction solvent, and greatly improve the recovery rate of active ingredients. The quality of the vanilla tincture prepared by the method is obviously better than that of the conventional tincture extraction method.
本发明所要解决的技术问题是通过以下技术方案来实现的：The technical problem to be solved by the present invention is achieved through the following technical solutions:
一种超声辅助加速溶剂萃取香荚兰酊剂的方法，它包括如下步骤：A method for ultrasonic-assisted accelerated solvent extraction of vanilla tincture, which comprises the steps of:
(1)将香荚兰冲洗除尘后进行剪切、分段处理；(1) Carry out shearing, subsection processing after the vanilla is rinsed and dedusted;
(2)向步骤(1)中处理好的香荚兰中加入质量比为1:1～1:5的乙醇溶剂，利用超声波细胞破碎仪进行预处理；(2) adding mass ratio to the vanilla processed in step (1) is an ethanol solvent of 1:1～1:5, and utilizes an ultrasonic cell disruptor to carry out pretreatment;
(3)将步骤(2)中超声破碎后的样品进行过滤，收集滤液，并保留滤渣待用；(3) Filter the sample after ultrasonic crushing in step (2), collect the filtrate, and keep the filter residue for later use;
(4)将步骤(3)中保留的滤渣转移至加速溶剂萃取的物料瓶中，萃取1～5次；(4) Transfer the filter residue retained in step (3) to the material bottle for accelerated solvent extraction, and extract 1 to 5 times;
(5)将步骤(4)所得的物料静置30～60min，得到澄清的液体；(5) leave the material obtained in step (4) for 30 to 60 minutes to obtain a clear liquid;
(6)将步骤(5)中所得的液体与步骤(3)中所收集的滤液混合，即为香荚兰酊剂。(6) Mix the liquid obtained in step (5) with the filtrate collected in step (3) to obtain vanilla tincture.
作为优选方案，所述步骤(1)中，对香荚兰进行分段处理，其中每段长度为1～2cm。As a preferred solution, in the step (1), the vanilla is subjected to section processing, wherein the length of each section is 1-2 cm.
作为优选方案，所述步骤(2)中，乙醇溶剂的体积浓度为50～100％。As a preferred solution, in the step (2), the volume concentration of the ethanol solvent is 50-100%.
作为优选方案，所述步骤(2)中超声波细胞破碎仪进行预处理的条件为：超声功率为200W～600W，超声时间为30min～90min，间歇时间为0～30s。As a preferred solution, the pretreatment conditions of the ultrasonic cell disruptor in the step (2) are as follows: the ultrasonic power is 200W-600W, the ultrasonic time is 30min-90min, and the intermittent time is 0-30s.
作为优选方案，所述步骤(4)中萃取条件为：功率设为200～600W，萃取时间为1h～3h，温度为50～100℃，压力设定为3～10MPa。As a preferred solution, the extraction conditions in the step (4) are as follows: the power is set at 200-600W, the extraction time is 1h-3h, the temperature is 50-100°C, and the pressure is set at 3-10MPa.
本发明的优点在于：The advantages of the present invention are:
1、本提取方法将香荚兰进行分段处理，有助于提取时香气成分溢出，能够提升有效成分的回收率。1. In this extraction method, the vanilla is processed in sections, which helps the aroma components to overflow during extraction and can improve the recovery rate of active ingredients.
2、本提取方法利用超声破碎技术，可以使细胞膜和细胞壁在超高压外力的作用下被破坏，使细胞内有效物质释放出来。2. This extraction method uses ultrasonic crushing technology, which can destroy the cell membrane and cell wall under the action of ultra-high pressure external force, so that the effective substances in the cell can be released.
3、本提取方法通过加速溶剂萃取技术，在高温高压下能够使溶剂更易进入样品基质内，使有效成分更容易扩散到溶剂中。3. The extraction method accelerates the solvent extraction technology, which can make the solvent more easily enter the sample matrix under high temperature and high pressure, and make it easier for the active ingredient to diffuse into the solvent.
4、该制备酊剂方法所需时间短、溶剂用量小，能够最大限度地对有效成分进行提取，所得酊剂品质明显优于其他传统方法。4. The method for preparing the tincture requires short time and a small amount of solvent, and can extract active ingredients to the greatest extent, and the quality of the obtained tincture is obviously better than other traditional methods.
具体实施方式Detailed ways
以下通过具体实施例对本发明作进一步的详细描述。The present invention will be described in further detail below through specific examples.
实施例一：Embodiment one:
一种超声辅助加速溶剂萃取香荚兰酊剂的方法，其步骤如下：A method for ultrasonic-assisted accelerated solvent extraction of vanilla tincture, the steps are as follows:
(1)取香荚兰50g，将其冲洗除尘后，剪切成长度为1cm的切断，备用；(1) Get vanilla 50g, after it is rinsed and dedusted, cut into lengths of 1cm and set aside;
(2)向步骤(1)中处理好的香荚兰中，加入50g体积浓度为60％的乙醇溶剂，利用超声波细胞破碎仪进行预处理：超声功率为200W，超声时间为30min，间歇时间为10s；(2) In the vanilla that has been processed in the step (1), add 50g volume concentration and be the ethanol solvent of 60%, utilize ultrasonic cell breaker to carry out pretreatment: ultrasonic power is 200W, and ultrasonic time is 30min, and intermittent time is 10s;
(3)将步骤(2)中超声破碎后的样品进行过滤，收集滤液，并保留滤渣待用；(3) Filter the sample after ultrasonic crushing in step (2), collect the filtrate, and keep the filter residue for later use;
(4)将步骤(3)中保留的滤渣转移至加速溶剂萃取的物料瓶中，功率设为200W，时间为10min，温度为60℃，压力设为3MPa，萃取2次；(4) Transfer the filter residue retained in step (3) to the material bottle for accelerated solvent extraction, set the power to 200W, the time to 10min, the temperature to 60°C, the pressure to 3MPa, and extract twice;
(5)将步骤(4)所得的物料静置30min，得到澄清的液体；(5) the material obtained in step (4) was left to stand for 30min to obtain a clear liquid;
(6)将步骤(5)中所得的液体与步骤(3)中所收集的滤液混合，即为香荚兰酊剂。(6) Mix the liquid obtained in step (5) with the filtrate collected in step (3) to obtain vanilla tincture.
实施例二：Embodiment two:
一种超声辅助加速溶剂萃取香荚兰酊剂的方法，其步骤如下：A method for ultrasonic-assisted accelerated solvent extraction of vanilla tincture, the steps are as follows:
(1)取香荚兰50g，将其冲洗除尘后，剪切成长度为1.5cm的切断，备用；(1) Get vanilla 50g, after it is washed and dedusted, cut into lengths of 1.5cm and cut off, for subsequent use;
(2)向步骤(1)中处理好的香荚兰中，加入80g体积浓度为80％的乙醇溶剂，利用超声波细胞破碎仪进行预处理：超声功率为400W，超声时间为60min，间歇时间为20s；(2) In the vanilla that has been processed in step (1), add 80g volume concentration and be 80% ethanol solvent, utilize ultrasonic cell breaker to carry out pretreatment: ultrasonic power is 400W, and ultrasonic time is 60min, and intermittent time is 20s;
(3)将步骤(2)中超声破碎后的样品进行过滤，收集滤液，并保留滤渣待用；(3) Filter the sample after ultrasonic crushing in step (2), collect the filtrate, and keep the filter residue for later use;
(4)将步骤(3)中保留的滤渣转移至加速溶剂萃取的物料瓶中，功率设为400W，时间为20min，温度为80℃，压力设为6MPa，萃取3次；(4) Transfer the filter residue retained in step (3) to the material bottle for accelerated solvent extraction, the power is set to 400W, the time is 20min, the temperature is 80°C, the pressure is set to 6MPa, and the extraction is performed 3 times;
(5)将步骤(4)所得的物料静置40min，得到澄清的液体；(5) the material obtained in step (4) was left to stand for 40min to obtain a clear liquid;
(6)将步骤(5)中所得的液体与步骤(3)中所收集的滤液混合，即为香荚兰酊剂。(6) Mix the liquid obtained in step (5) with the filtrate collected in step (3) to obtain vanilla tincture.
实施例三：Embodiment three:
一种超声辅助加速溶剂萃取香荚兰酊剂的方法，其步骤如下：A method for ultrasonic-assisted accelerated solvent extraction of vanilla tincture, the steps are as follows:
(1)取香荚兰50g，将其冲洗除尘后，剪切成长度为2cm的切断，备用；(1) get vanilla 50g, after it is rinsed and dedusted, cut into lengths of 2cm and cut off, for subsequent use;
(2)向步骤(1)中处理好的香荚兰中，加入100g无水乙醇溶剂，利用超声波细胞破碎仪进行预处理：超声功率为600W，超声时间为90min，间歇时间为20s；(2) In the vanilla treated in step (1), add 100g of absolute ethanol solvent, and utilize an ultrasonic cell breaker to carry out pretreatment: the ultrasonic power is 600W, the ultrasonic time is 90min, and the intermittent time is 20s;
(3)将步骤(2)超声破碎后的样品进行过滤，收集滤液，并保留滤渣待用；(3) filtering the sample after step (2) ultrasonic crushing, collecting the filtrate, and retaining the filter residue for later use;
(4)将步骤(3)中保留的滤渣转移至加速溶剂萃取的物料瓶中，功率设为600W，时间为30min，温度为100℃，压力设为9MPa，萃取4次；(4) Transfer the filter residue retained in step (3) to the material bottle for accelerated solvent extraction, the power is set to 600W, the time is 30min, the temperature is 100°C, the pressure is set to 9MPa, and the extraction is performed 4 times;
(5)将步骤(4)所得的物料静置60min，得到澄清的液体；(5) The material obtained in step (4) is left to stand for 60min to obtain a clear liquid;
(6)将步骤(5)中所得的液体与步骤(3)中所收集的滤液混合，即为香荚兰酊剂。(6) Mix the liquid obtained in step (5) with the filtrate collected in step (3) to obtain vanilla tincture.
实施例四：Embodiment four:
一种超声辅助加速溶剂萃取香荚兰酊剂的方法，其步骤如下：A method for ultrasonic-assisted accelerated solvent extraction of vanilla tincture, the steps are as follows:
(1)取香荚兰50g，将其冲洗除尘后，剪切成长度为2cm的切断，备用；(1) get vanilla 50g, after it is rinsed and dedusted, cut into lengths of 2cm and cut off, for subsequent use;
(2)向步骤(1)中处理好的香荚兰中，加入50g无水乙醇溶剂，利用超声波细胞破碎仪进行预处理：超声功率为600W，超声时间为90min，间歇时间为20s；(2) In the vanilla treated in step (1), add 50g of absolute ethanol solvent, and utilize an ultrasonic cell disruptor to carry out pretreatment: the ultrasonic power is 600W, the ultrasonic time is 90min, and the intermittent time is 20s;
(3)将步骤(2)超声破碎后的样品进行过滤，收集滤液，并保留滤渣待用；(3) filtering the sample after step (2) ultrasonic crushing, collecting the filtrate, and retaining the filter residue for later use;
(4)将步骤(3)中保留的滤渣转移至加速溶剂萃取的物料瓶中，功率设为600W，时间为30min，温度为100℃，压力设为9MPa，萃取4次；(4) Transfer the filter residue retained in step (3) to the material bottle for accelerated solvent extraction, the power is set to 600W, the time is 30min, the temperature is 100°C, the pressure is set to 9MPa, and the extraction is performed 4 times;
(5)将步骤(4)所得的物料静置60min，得到澄清的液体；(5) The material obtained in step (4) is left to stand for 60min to obtain a clear liquid;
(6)将步骤(5)中所得的液体与步骤(3)中所收集的滤液混合，即为香荚兰酊剂。(6) Mix the liquid obtained in step (5) with the filtrate collected in step (3) to obtain vanilla tincture.
实施例五：Embodiment five:
一种超声辅助加速溶剂萃取香荚兰酊剂的方法，其步骤如下：A method for ultrasonic-assisted accelerated solvent extraction of vanilla tincture, the steps are as follows:
(1)取香荚兰50g，将其冲洗除尘后，剪切成长度为1.5cm的切断，备用；(1) Get vanilla 50g, after it is washed and dedusted, cut into lengths of 1.5cm and cut off, for subsequent use;
(2)向步骤(1)中处理好的香荚兰中，加入200g体积浓度为80％的乙醇溶剂，利用超声波细胞破碎仪进行预处理：超声功率为400W，超声时间为60min，间歇时间为20s；(2) In the vanilla that has been processed in step (1), add 200g volume concentration and be the ethanol solvent of 80%, utilize ultrasonic cell breaker to carry out pretreatment: ultrasonic power is 400W, and ultrasonic time is 60min, and intermittent time is 20s;
(3)将步骤(2)中超声破碎后的样品进行过滤，收集滤液，并保留滤渣待用；(3) Filter the sample after ultrasonic crushing in step (2), collect the filtrate, and keep the filter residue for later use;
(4)将步骤(3)中保留的滤渣转移至加速溶剂萃取的物料瓶中，功率设为400W，时间为20min，温度为80℃，压力设为6MPa，萃取3次；(4) Transfer the filter residue retained in step (3) to the material bottle for accelerated solvent extraction, the power is set to 400W, the time is 20min, the temperature is 80°C, the pressure is set to 6MPa, and the extraction is performed 3 times;
(5)将步骤(4)所得的物料静置40min，得到澄清的液体；(5) the material obtained in step (4) was left to stand for 40min to obtain a clear liquid;
(6)将步骤(5)中所得的液体与步骤(3)中所收集的滤液混合，即为香荚兰酊剂。(6) Mix the liquid obtained in step (5) with the filtrate collected in step (3) to obtain vanilla tincture.
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| CN109105947A (en) * | 2018-07-05 | 2019-01-01 | 湖北中烟工业有限责任公司 | A kind of multistage solvent extraction preparation method and applications of cocoa extract |
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| CN101498701A (en) * | 2009-03-09 | 2009-08-05 | 南京大学 | Detection method for phthalate ester residue |
| US20100058973A1 (en) * | 2006-11-15 | 2010-03-11 | Skin'up | Control and/or monitoring method for the salting-out of at least one active ingredient impregnated in a textile |
| CN103525558A (en) * | 2013-09-27 | 2014-01-22 | 福建中烟工业有限责任公司 | Tea flower essence with fruity flavor characteristic and application thereof to cigarettes |
| CN103783226A (en) * | 2014-02-19 | 2014-05-14 | 郭景龙 | Soy sauce flavor type tea |
-
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- 2015-02-28 CN CN201510092733.7A patent/CN104740893B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100058973A1 (en) * | 2006-11-15 | 2010-03-11 | Skin'up | Control and/or monitoring method for the salting-out of at least one active ingredient impregnated in a textile |
| CN101498701A (en) * | 2009-03-09 | 2009-08-05 | 南京大学 | Detection method for phthalate ester residue |
| CN103525558A (en) * | 2013-09-27 | 2014-01-22 | 福建中烟工业有限责任公司 | Tea flower essence with fruity flavor characteristic and application thereof to cigarettes |
| CN103783226A (en) * | 2014-02-19 | 2014-05-14 | 郭景龙 | Soy sauce flavor type tea |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2642641C1 (en) * | 2016-11-16 | 2018-01-25 | федеральное государственное бюджетное образовательное учреждение высшего образования "Алтайский государственный университет" | Membrane extractor |
| CN109105947A (en) * | 2018-07-05 | 2019-01-01 | 湖北中烟工业有限责任公司 | A kind of multistage solvent extraction preparation method and applications of cocoa extract |
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