CN104740335A - Dendrobium officinale leaf extract with uric acid reducing function and application of dendrobium officinale leaf extract - Google Patents
Dendrobium officinale leaf extract with uric acid reducing function and application of dendrobium officinale leaf extract Download PDFInfo
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- CN104740335A CN104740335A CN201310753697.5A CN201310753697A CN104740335A CN 104740335 A CN104740335 A CN 104740335A CN 201310753697 A CN201310753697 A CN 201310753697A CN 104740335 A CN104740335 A CN 104740335A
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- leaf extract
- dendrobium officinale
- herba dendrobii
- officinale leaf
- uric acid
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Abstract
The invention discloses a dendrobium officinale leaf extract with a uric acid reducing function and application of the dendrobium officinale leaf extract. The dendrobium officinale leaf extract is prepared through the following steps: cleaning and drying the dendrobium officinale leaves; mechanically pulverizing; carrying out reflux extraction by a 0-30V% ethanol solution for 1-3 times, wherein the time of each reflux extraction is 1-3h and the dosage of each reflux extraction is 5-20 times of the medicine weight; combining the filtrates; recovering the ethanol; and carrying out vacuum concentration to obtain the dendrobium officinale leaf extract. The dendrobium officinale leaf extract can be used for preventing medicines or food for preventing and treating hyperuricemia; the dendrobium officinale leaf extract relies on the dendrobium officinale leaves to develop the effects of the dendrobium officinale leaves, so that the new medicinal resources of the dendrobium officinale are expanded and the resource waste is decreased at the same time.
Description
Technical field
The invention belongs to medical art, particularly relate to a kind of Herba Dendrobii leaf extract and the purposes with uric acid resisting effect.
Background technology
The sickness rate showed increased in recent years of gout and hyperuricemia, the sickness rate of hyperuricemia reaches 10%, and the pathogenesis basis of gout is hyperuricemia, is because dietary structure is uneven, causes many kinds of substance metabolism disorder in human body and the disease that causes.Just damage kidney and other organs, the renal calculus of clinical findings unknown cause, the patient of renal failure before a large amount of Patients with Hyperuricemia generation gouts, one of its cause of disease is exactly caused by long-term blood uric acid (UA) increases.So find that the patient that UA obviously raises must do uric acid resisting process.In recent years much research shows, hyperuricemia is relevant to the generation of hypertension, impaired glucose tolerance, central obesity, disorders of lipid metabolism, atherosclerosis etc.Along with socioeconomic development and growth in the living standard, the improvement of people's nutritional condition, take in animal proteinum, the increase of fat and the change (drink, smoking etc.) of life-form structure, obese people increases, and the patient of hyperuricemia also increases year by year.Hyperuricemia is divided into generation to increase type and excretion reduction type according to the origin cause of formation by modern medicine, and selects suppression uricopoiesis medicine as allopurinol according to renal function situation, or promotes that urate excretion medicine is as benzbromarone, probenecid, sulphinpyrazone.Although these medicines have obvious uric acid resisting effect, side effect is also comparatively large, as erythra, heating, hepatic renal dysfunction, hemopoietic function exception, gout outbreak etc., thus limits the scope of its application.Therefore, developmental research drug effect is clear and definite and the novel anti-antihyperuricemic disease drug of safety is very necessary.The maximum characteristics and advantages of Chinese medicine is organic conception, and it, by Mutiple Targets, multipath, makes uric acid steadily decline, produce certain Renoprotective Effect.Therefore treatment by Chinese herbs metabolic arthritis is to improving the quality of life of patient and improving prognosis and have very large advantage.
The dry stem of Herba Dendrobii Dendrobium officinale Kimura et Migo, sweet in the mouth, is slightly cold.Return stomach, kidney channel, there is reinforcing stomach reg fluid, nourishing YIN and clearing away heat.
Herba Dendrobii leaf, does not belong to medicinal part at present, can not use as bread and cheese; But output is very large, and research shows, Herba Dendrobii leaf and Herba Dendrobii medical material (stem), containing multiple types chemical composition together, also have the function that part is similar, but the huge Herba Dendrobii leaf of current production rate can only do refuse process.There is no the medicinal part Herba Dendrobii leaf recorded during the present invention selects " Chinese Pharmacopoeia " as support, develop its effect, expand the medicine resource that Herba Dendrobii is new, decrease the waste of resource simultaneously.Inventor is found by research, and Herba Dendrobii leaf extract can reduce rat model serum uric acid level, can be used for preventing and treating medicine and health food that hyperuricemia is major function.
Summary of the invention
The object of the invention is to for the deficiencies in the prior art, a kind of Herba Dendrobii leaf extract and the purposes with uric acid resisting effect are provided.
The present invention solves the problems of the technologies described above adopted technical scheme: a kind of Herba Dendrobii leaf extract with uric acid resisting effect, described Herba Dendrobii leaf extract prepares by the following method: Herba Dendrobii leaf is cleaned, after drying, mechanical activation comminution, alcohol reflux with 0 ~ 30% 1 ~ 3 time, each 1 ~ 3h, each consumption is respectively 5 ~ 20 times of medical material weight, merging filtrate, and concentrating under reduced pressure obtains Herba Dendrobii leaf extract.
This Herba Dendrobii leaf extract can be used for preparing the hypertensive medicine of prevention and therapy or food.This Herba Dendrobii leaf extract can with the adjuvant approved on medicament or food, adopt Modern preparations technology, make based on the extractum of Herba Dendrobii leaf extract, the various dosage forms such as tablet, granule, pill, capsule, powder, oral liquid.
The invention has the beneficial effects as follows there is no the medicinal part Herba Dendrobii leaf recorded during the present invention selects " Chinese Pharmacopoeia " as support, develop its effect, expand the medicine resource that Herba Dendrobii is new, decrease the waste of resource simultaneously.The experiment of inventor shows: this Herba Dendrobii leaf extract significantly can reduce the serum uric acid level of rat model, can be used for preventing and treating medicine and health food that hyperuricemia is major function.
Detailed description of the invention
Further describe the present invention according to embodiment below, effect of the present invention will be reflected more clearly.
Embodiment 1
Herba Dendrobii leaf is cleaned, after drying, mechanical activation comminution, adding 5 times amount concentration of volume percent is the alcoholic solution of 30%, and extract 3 times, each 1h, merging filtrate, concentrating under reduced pressure obtains Herba Dendrobii leaf extract, and 4 DEG C of refrigerator cold-storages are for subsequent use.
Embodiment 2
Herba Dendrobii leaf is cleaned, after drying, mechanical activation comminution, adds 15 times of water gagings, and extract 2 times, each 2 hours, merging filtrate, concentrating under reduced pressure obtains Herba Dendrobii leaf extract, and 4 DEG C of refrigerator cold-storages are for subsequent use.
Embodiment 3
Herba Dendrobii leaf is cleaned, after drying, mechanical activation comminution, adds 20 times amount 15% alcoholic solution, extracts 3 hours, and filtration, filtrate reduced in volume obtains Herba Dendrobii leaf extract, and 4 DEG C of refrigerator cold-storages are for subsequent use.
Embodiment 4
The serum uric acid level of the Hypertensive Rats that Herba Dendrobii dried leaves water extract is drunk to chronic stress combination through long term administration discovery, prepared by high glucose and high fat has significant reducing effect.
1 experiment material and instrument
1.1 materials and instrument
1.1 animal SD rats, male and female half and half, body weight (200 ~ 550g), 40.The quality certification number: scxk (Shanghai) 20080033.
1.2 Pharmaceutical formulations
1.2.1 by reagent
Herba Dendrobii leaf water extract high dose 5g/kg, middle dosage 2.5g/kg, low dosage 1.25g/kg.4 DEG C of refrigerator cold-storages are for subsequent use.
1.2.2 positive drug Captopril tablets, specification 12.5mg/ sheet, lot number 120618, Henan Zhong Jie pharmaceutcal corporation, Ltd, being mixed with concentration with distilled water is 0.015g/kg.
1.3 electronics balance CP114 (Shanghai Ao Haosi Instrument Ltd.); YP15KN-electronic weighing balance (Shanghai Hengping Science Instrument Co., Ltd.); YF-40 traditional Chinese medicine extracting machine (the former medical treatment in East China, Beijing); The multiplex vacuum pump of SHB-B95A type circulating water type (Shanghai Yu Kang Science & Teaching Instrument equipment company limited); GG-17 Rotary Evaporators (Shanghai Yu Kang Science & Teaching Instrument equipment company limited); Lifting thermostat water bath (Hangzhou David's Science & Teaching Instrument company) DLSB-30/30 DEG C sub-cooled circulating pump (Hangzhou Hui Chuan instrument and equipment company limited); CM-230 pure water instrument (Hangzhou Yongjieda Cleaning Technology Co., Ltd); KQ-500DE type numerical control ultrasonic cleaner (Kunshan Ultrasonic Instruments Co., Ltd.); 501-thermostatted water oil bath pan, SHB-III circulating water type vacuum pump, DLSB--5/10 DEG C sub-cooled circulating pump (Hangzhou Hui Chuan instrument and equipment company limited); TBA-A0FR (ACCUTE) automatic clinical chemistry analyzer (wide medical Co., Ltd. of Japanese Toshiba three).
2 experimental techniques
60 rat models are divided into normal group according to body weight by 2.1 administration leaves at random, model control group, dosage (2.5g/kg), Herba Dendrobii dried leaves low dosage (1.25g/kg) in Herba Dendrobii dried leaves high dose (5g/kg) group, Herba Dendrobii dried leaves, normal group gives purified water, normal diet, model group and administration group administration Chinese liquor, high glucose and high fat feedstuff.Gastric infusion, model group and normal group gavage give tap water, and administration group gives corresponding medicine, and administration capacity is 1ml/100g body weight.
2.2 observation index successive administrations 14 weeks, get hematometry rat blood serum uric acid (UA) level in every 2 weeks.
2.3 statistics with analyze measure data result with
represent, group difference adopts t-test.
3 experimental results
Normal group, model group and each administration group blood pressure situation during 3.1 administrations
Be combined than chronic stress the uric acid preparing hypertension model with high glucose and high fat of drinking with normal group obviously to raise (P<0.01); With model group ratio, administration 4 weeks Herba Dendrobii dried leaves water extract high doses significantly can reduce rat model UA level (P<0.01), and uric acid resisting effect steadily continues.The results are shown in Table 1.
The change of table 1 successive administration rat UA level (
n=10)
Note: with normal group ratio, * P<0.05, * * P<0.01; Compare with model control group,
△p<0.05,
△ △p<0.01.
Claims (2)
1. one kind has the Herba Dendrobii leaf extract of uric acid resisting effect, it is characterized in that, described Herba Dendrobii leaf extract prepares by the following method: Herba Dendrobii leaf is cleaned, after drying, mechanical activation comminution is the alcoholic solution reflux, extract, 1 ~ 3 time of 0 ~ 30% with concentration of volume percent, each 1 ~ 3h, each consumption is respectively 5 ~ 20 times of medical material weight, merging filtrate, reclaim ethanol, concentrating under reduced pressure obtains Herba Dendrobii leaf extract.
2. the purposes of Herba Dendrobii leaf extract in the medicine preparing prevention and therapy hyperuricemia or food described in a claim 1.
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| CN201310753697.5A CN104740335A (en) | 2013-12-30 | 2013-12-30 | Dendrobium officinale leaf extract with uric acid reducing function and application of dendrobium officinale leaf extract |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105685186A (en) * | 2016-03-31 | 2016-06-22 | 浙江科技学院 | Dendrobium officinale leaf biscuit and production method thereof |
| CN106039027A (en) * | 2016-06-23 | 2016-10-26 | 云南润紫源生物科技股份有限公司 | Dendrobium candidum leaf lozenge as well as preparation method and application thereof |
| CN106377672A (en) * | 2015-10-14 | 2017-02-08 | 重庆蹇家坪农业科技发展有限公司 | Preparation method for extract of Folium Dendrobium officinale |
| CN108310227A (en) * | 2018-04-01 | 2018-07-24 | 张奉明 | A kind of dendrobium candidum composition and preparation method thereof of beneficial bony articulation health |
| CN109663062A (en) * | 2018-09-16 | 2019-04-23 | 瓦房店市祖御康生物富硒产品销售处 | A kind of selenium-rich peptide dendrobium nutrient alkali and its boiling method, application |
| CN112057559A (en) * | 2020-10-10 | 2020-12-11 | 中国药科大学 | Application of dendrobium huoshanense extract in medicine for preventing, relieving and/or treating uric acid renal disease |
| CN115399444A (en) * | 2022-09-28 | 2022-11-29 | 清华大学无锡应用技术研究院 | Method for making bean product suitable for people with high uric acid |
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| CN102743421A (en) * | 2012-07-12 | 2012-10-24 | 万福群 | Synsepalum dulcificum Denill tree branch and leaf extract and extraction method and application of extract |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106377672A (en) * | 2015-10-14 | 2017-02-08 | 重庆蹇家坪农业科技发展有限公司 | Preparation method for extract of Folium Dendrobium officinale |
| CN105685186A (en) * | 2016-03-31 | 2016-06-22 | 浙江科技学院 | Dendrobium officinale leaf biscuit and production method thereof |
| CN106039027A (en) * | 2016-06-23 | 2016-10-26 | 云南润紫源生物科技股份有限公司 | Dendrobium candidum leaf lozenge as well as preparation method and application thereof |
| CN108310227A (en) * | 2018-04-01 | 2018-07-24 | 张奉明 | A kind of dendrobium candidum composition and preparation method thereof of beneficial bony articulation health |
| CN109663062A (en) * | 2018-09-16 | 2019-04-23 | 瓦房店市祖御康生物富硒产品销售处 | A kind of selenium-rich peptide dendrobium nutrient alkali and its boiling method, application |
| CN112057559A (en) * | 2020-10-10 | 2020-12-11 | 中国药科大学 | Application of dendrobium huoshanense extract in medicine for preventing, relieving and/or treating uric acid renal disease |
| CN115399444A (en) * | 2022-09-28 | 2022-11-29 | 清华大学无锡应用技术研究院 | Method for making bean product suitable for people with high uric acid |
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