CN104740077A - Traditional Chinese medicine preparation for treating tumor diseases and preparation method thereof - Google Patents
Traditional Chinese medicine preparation for treating tumor diseases and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a traditional Chinese medicine preparation for treating tumor diseases and a preparation method thereof. The traditional Chinese medicine preparation comprises, by weight, 50 parts of red ginseng, 100 parts of radix astragali, 100 parts of wolfberry, 100 parts of glossy privet fruit, 150 parts of polyporus and 100 parts of poria cocos. Through a certain extraction purification process, the above materials and pharmaceutically acceptable accessory materials are processed to form a traditional Chinese medicine oral preparation. The traditional Chinese medicine preparation has the characteristics of simple and feasible preparation technology, safety, effectiveness, obvious pharmacological effects and substantial clinical effects.
Description
Technical field
The present invention relates to a kind of Chinese medicine preparation for the treatment of oncosis and preparation method thereof, belong to pharmaceutical preparations technology field.
Background technology
Malignant tumor is a kind of serious threat human life and healthy major disease, and sickness rate has the trend risen year by year.At present, to the treatment of this type of disease usually with X-ray therapy and chemotherapy.Wherein X-ray therapy has the effect of killing local tumor cell, but the patient shifted for cancer is just not helpless.Chemotherapy utilizes the ability of chemical substance interfere with cancer cells repetition DNA stop its increment or bring out its death, and normal tissue cell and organ have obvious damage effect simultaneously.If patient uses these class methods and medicine to treat for a long time, the side reaction such as bone marrow depression, immunologic hypofunction can be caused, and easily produce drug resistance; After cancer patient's chemicotherapy of about more than 90%, occur that leukopenic symptom is particularly common.Given this, seek good effect, medicine tool that toxic and side effects is little is of great significance.At present, clinical therapy of tumor there is no desirable treatment means, but Chinese medicine has longer history in this type of disease for the treatment of, and with its advantage that medicine source is extensive, cheap, toxic and side effects is little.
Chinese medicine is thought, often has that consumption impairment of QI is cloudy, taste are impaired in chemotherapy process, causes human bone marrow to suppress, hypoimmunity, affects the function of the raw and the kidney generating marrow and dominating bone of QI and bloodization.Antineoplastic Chinese medicine has YIN nourishing, QI invigorating, spleen invigorating, reaches QI and blood regulating, internal organs regulating YIN and YANG, QI and blood is grown, and to correct the QI consumed the moon wound in chemotherapy process, impaired taste is repaired.Radix Ginseng strongly invigorating primordial QI in medicine of the present invention, Radix Astragali qi-supplementing, blood-engendering, mutual reinforcement between is use, and the power benefit of benefiting qi and nourishing blood is evident, is monarch drug altogether.Fructus Lycii liver and kidney tonifying; Fructus Ligustri Lucidi nourishing YIN and clearing away heat, share the power benefit work of then YIN nourishing, with being ministerial drug.Poria spleen invigorating eliminating dampness by diuresis, Polyporus promoting diuresis to eliminate damp pathogen, the merit share then diuretic doubly, makes all medicine tonifys without causing stagnation, makes with for assistant.All medicines amount to mutually and play replenishing QI to invigorate the spleen, the merit of nourishing the liver and kidney.
Between this; the applicant's earlier application patent of invention; its publication number is CN1544078A; publication date is on April 27th, 2004; this patent is from prescription flavour of a drug mediating recipe amount; preparation technology and dosage form are protected; but in clinical practice application process; we find that the therapeutic effect of the Chinese medicine preparation be made up of above-mentioned preparation method is desirable not enough; and the water extracted immersing paste powder of oral formulations in actual production process has stronger hygroscopicity; and liquid preparation has deposited phenomenon in long-term put procedure, affect the stability of Chinese medicine preparation.And the application's patent is groped by great many of experiments, further optimized fabrication process, and have found better preparation technology's flow process and parameter, to improve for the purpose of Chinese medicine preparation stability of the present invention and bioavailability, and Chinese medicine preparation of creationary treatment oncosis of succeeding in developing and preparation method thereof.
Summary of the invention
The object of the invention is to provide a kind of taking convenience, steady quality, curative effect Chinese medicine preparation for the treatment of oncosis and preparation method thereof reliably.
Technical solution of the present invention is a kind of Chinese medicine preparation being used for the treatment of oncosis, and it is made up of the raw material of following parts by weight: Radix Ginseng Rubra 50 parts, the Radix Astragali 100 parts, Fructus Lycii 100 parts, Fructus Ligustri Lucidi 100 parts, Polyporus 150 parts, 100 parts, Poria;
The method preparation in accordance with the following steps of above-mentioned medical material:
(1) Polyporus, Poria powder are broken into fine powder, for subsequent use;
(2) get Radix Ginseng Rubra medical material, be ground into coarse powder, by medical material weight ratio 7 ~ 10 times amount, concentration be 50% ~ 80% ethanol carry out seepage pressure effects, collect percolate and also reclaim ethanol, concentrate drying is also ground into dried cream powder, and for subsequent use, medicinal residues are continued to employ;
By step (2) in medicinal residues merge with the Radix Astragali, Fructus Lycii, Fructus Ligustri Lucidi again, add the soak by water 1 ~ 3 time that medical material weight ratio is 8 ~ 11 times amount, each 1 ~ 2h, filter, merging filtrate, concentrating under reduced pressure become relative density be 1.10 clear paste, add ethanol to alcohol content 50% ~ 80%, cold preservation leaves standstill 12 ~ 36h, filters, filtrate recycling ethanol and through concentrating under reduced pressure become relative density be 1.20 clear paste, add step (1) in the medicated powder for subsequent use of 2/3 amount, mixing, is dryly also ground into dried cream powder, sieve for subsequent use;
(4) get above-mentioned steps (1) in medicated powder remaining for subsequent use, and step (2), (3) in dried cream powder, fully after mixing, obtain Traditional Chinese drug mixture, then add adjuvant conventional on pharmaceutics and make various different oral formulations, to obtain final product.
Wherein preferred preparation method is realized by following steps:
(1) Polyporus, Poria powder are broken into fine powder, for subsequent use;
(2) get Radix Ginseng Rubra medical material, be ground into coarse powder, by medical material weight ratio 8 times amount, concentration be 70% ethanol carry out seepage pressure effects, collect percolate and also reclaim ethanol, concentrate drying is also ground into dried cream powder, and for subsequent use, medicinal residues are continued to employ;
By step (2) in medicinal residues merge with the Radix Astragali, Fructus Lycii, Fructus Ligustri Lucidi again, add the soak by water 2 times that medical material weight ratio is 9 times amount, each 1.5h, filter, merging filtrate, concentrating under reduced pressure become relative density be 1.10 clear paste, add ethanol to alcohol content 70%, cold preservation leaves standstill 24h, filters, filtrate recycling ethanol and through concentrating under reduced pressure become relative density be 1.20 clear paste, add step (1) in the medicated powder for subsequent use of 2/3 amount, mixing, is dryly also ground into dried cream powder, sieve for subsequent use;
(4) get above-mentioned steps (1) in remaining medicated powder for subsequent use, and step (2), (3) in dried cream powder, fully after mixing, obtain Traditional Chinese drug mixture, then add adjuvant conventional on pharmaceutics and make various different oral formulations, to obtain final product.
The oral formulations that technical solution of the present invention is made can be granule, hard capsule, tablet or drop pill.
Drop pill: by gained Traditional Chinese drug mixture, stirs with suitable substrate hot melt, and carries out dripping, make drop pill.
Granule: by gained Traditional Chinese drug mixture, mixes with appropriate dextrin, sucrose, granulates, dry, makes granule.
Oral solutions: by gained Traditional Chinese drug mixture, mixes with appropriate correctives, antiseptic, adds water and make oral liquid.
Hard capsule: by gained Traditional Chinese drug mixture, adds suitable adjuvant and granulates, encapsulated, makes hard capsule.
The present invention is in the middle of practical study process, and the main creation point of technical scheme is the extraction process of medical material, and bring significant technological progress thus, its main beneficial effect is as follows.
Chinese medicine preparation of the present invention is prepared from by Radix Ginseng Rubra, the Radix Astragali, Fructus Lycii, Fructus Ligustri Lucidi, Polyporus, Poria 6 taste Chinese crude drug.Modern pharmacy research shows, contains a large amount of polysaccharide, starch, triterpene, fatty acid, sterol enzyme composition in Polyporus and Poria medical material, because polysaccharide effective ingredient is soluble in water, therefore adopts decocting to extract.But find starch gelatinizing phenomenon in decoction process in its medical material, therefore become fine powder to be used as medicine with Poria pulverizing medicinal materials Polyporus, first both medical material effective ingredient all can be retained, the consumption adding adjuvant can be reduced in production process again.And in side, in Radix Ginseng Rubra, contain the compositions such as ginsenoside, organic acid, sterol and volatile oil, in research process, decocting method, ethanol refluxing process, percolation are compared, find that percolation extracts the active constituent content obtained higher; Polysaccharide, saponin, flavone, aminoacid and trace element is mainly contained in Milkvetch Root; Containing betanin, polysaccharide, vitamin B in Fructus Lycii
1, vitamin B
2, vitamin C, calcium, phosphorus, ferrum, cupreol, linoleic acid, and several amino acids.Its main active of Fructus Ligustri Lucidi is ursolic acid, oleanolic acid etc.Because in these medical materials, active component is all soluble in water, therefore adopt decocting extraction.In order to adopt decoction and alcohol sedimentation technique to process protein, phlegmatic temperament, tannin, pigment, removing in water boiling and extraction, the content of active component in further purification Chinese medicine extract, to reach the curative effect of medicine the best.Preparation technology of the present invention and original process distinction are: the part fine powder after the pulverizing of Poria and Polyporus is added the extractum that decocting extracts, and carry out vacuum drying, the improvement of this scheme, the vacuum drying time compares than originally and decreases 30%, and the color and luster of the extract powder obtained is relatively more even, is conducive to subsequent treatment operation, reduce production cost, and obtain the stable Chinese medicine preparation of quality comparation, ensure the curative effect of pharmaceutical preparation.
In a word, the technical scheme of extraction and preparation technique of the present invention and molding is that we are in the research process of reality, groped and accidental discovery by unremitting, and the clinical practice demand of said preparation of comprehensively having investigated, rational pool, comparison and the process route drawn, thus ensure that science and the effectiveness of the Chinese medicine preparation finally made.
In order to the reasonability of preparation technology of the present invention is described, we are in technical study in early stage, and also carry out relevant pharmaceutical test, particular content is as follows:
1.1 pairs of Radix Ginseng Rubra craft screenings:
With decocting method, ethanol refluxing process, percolation, Study on Extraction Method is carried out to Radix Ginseng Rubra respectively, Radix Ginseng total saponins (Rg in Detection and Extraction liquid
1and Re) content, concrete operation method is as follows: 1. decocting method: get Radix Ginseng Rubra coarse powder 50g, and 8 times amount that add water respectively decoct 2 times, 2h/ time, filters, merging filtrate, concentrating under reduced pressure, dry dry extract; 2. ethanol refluxing process: get Radix Ginseng Rubra coarse powder 50g, adds 8 times amount 70% alcohol reflux 2 times, 2h/ time respectively, filters, merging filtrate, concentrating under reduced pressure, dry dry extract; 3. percolation: get Radix Ginseng Rubra coarse powder 50g, with loading in percolator after 70% ethanol as solvent moistening, after solubilizer dipping 24h, starting percolation, controlling percolation speed 2ml/ (kgmin), collecting percolate 400ml, concentrating under reduced pressure, dry dry extract.
The preparation of need testing solution: get the dried cream powder end that three kinds of distinct methods extract, mill, after sieving, precision weighing 0.5g, adds chloroform 100ml, reflux 2h, backflow volatilizes chloroform and obtains medicinal residues, adds water saturated n-butyl alcohol 40ml, close plug, placement is spent the night, supersound process 30min, filters, precision measures filtrate 25ml, puts in evaporating dish, evaporate to dryness, residue adds dissolve with methanol and is transferred in measuring bottle, adds methanol to scale, shakes up and get final product.The chromatographic condition of total Ginsenosides Content: anti-phase C
18post (4.6mm × 250mm, 5 μm); Acetonitrile: 0.05% phosphoric acid (103: 357) is mobile phase; Flow velocity: 1ml/min; Determined wavelength 203nm.Number of theoretical plate calculates should be not less than 3000 with ginsenoside Re peak.The results are shown in Table 1.
The research of table 1 Radix Ginseng Rubra Different Extraction Method is compared
Experimental result table 1 is known: dry extract yield and the total Ginsenosides Content of percolation extraction are the highest, and suitability for industrialized production is easy, and therefore the present invention adopts percolation as the extraction process of Radix Ginseng Rubra.
The preparation of 1.2 medicines of the present invention and quality testing:
1.2.1 the preparation of medicine of the present invention:
(1), by Polyporus 150g, Poria 100g, is ground into fine powder, for subsequent use;
(2) get Radix Ginseng Rubra medical material 50g, be ground into coarse powder, use 8 times amount, concentration be 70% ethanol carry out seepage pressure effects, collect percolate and also reclaim ethanol, concentrate drying is also ground into dried cream powder, and for subsequent use, medicinal residues are continued to employ;
By step medicinal residues (2) again with Radix Astragali 100g, Fructus Lycii 100g, Fructus Ligustri Lucidi 100g, add the soak by water 2 times that medical material weight ratio is 9 times amount, each 1.5h, filter, merging filtrate, concentrating under reduced pressure become relative density be 1.10 clear paste, add ethanol to alcohol content 70%, cold preservation leaves standstill 24h, filters, filtrate recycling ethanol and through concentrating under reduced pressure become relative density be 1.20 clear paste, add step (1) in the medicated powder of 2/3 amount, mixing, vacuum drying becomes dry cream, and be ground into dried cream powder, sieve for subsequent use;
(4) get above-mentioned steps (1) in remaining medicated powder, and step (2), (3) in dried cream powder, fully after mixing, to obtain final product.
1.2.2 the hygroscopicity of medicine of the present invention, extractum and assay
1. get medicine 2g relative humidity 75% of the present invention and temperature is under the condition of 25 DEG C, gravimetry, draws its sucting wet curve, and calculates critical relative humidity (CRH); And it is appropriate to get medicine of the present invention, according to " Chinese Pharmacopoeia " 2010 editions, the assay method under water-soluble extractives (annex XA) item, measures; 2. adopting phend-sulphuric acid, take glucose as reference substance, measures the content of total polysaccharides; 3. medicine of the present invention is got appropriate, adopt " under 1.1, the chromatographic condition of total Ginsenosides Content: " measure, ensure the quality of medicine of the present invention, experimental data refers to table 2.
Table 2 medicine hygroscopicity of the present invention, extractum and Content inspect
From experimental result 2, the resistance to water soak of medicine of the present invention strengthens, and shows the good stability of medicine of the present invention, and the water-soluble extractives of medicine increases, and in medicine, polysaccharide and total Ginsenosides Content also increase, and ensures the performance of curative effect of medication further.
In sum, pharmaceutical preparation of the present invention, has scientific and reasonable, feasible, is conducive to technology and produces.And cost in production process can be reduced, and the stability of medicine can be improved.In order to further illustrate the superiority of technical solution of the present invention, we carry out pharmacology test to verify, concrete experiment content is as follows.
1 test material and method:
1.1 trial drugs:
A. the preparation of medicine group of the present invention:
Before use, by being mixed with the suspendible medicinal liquid of product group equivalent concentration in the middle of same medicine of the present invention according to " medicine of the present invention prepared under the item of 1.2.1 " adding distil water, to obtain final product.
B. the preparation of matched group medicine:
Before use, adding distil water is mixed with the suspendible medicinal liquid of product group equivalent concentration in the middle of same medicine of the present invention, to obtain final product.1.2 experimental animals:
Kunming kind rat, male, body weight is 20 ± 2g, mice S
180cell strain provides by The Fourth Military Medical University's animal center.
1.3 statistical procedures: experimental result with
represent, adopt t inspection, measure the significance of group difference.2. the test of pesticide effectiveness and result:
2.1 couples of mice transplanted sarcoma S
180the impact of tumor weight and tumour inhibiting rate
Get abdominal part inoculation S
180the tumor-bearing mice of tumor strain, extracts abdominal cavity tumor liquid under sterile working, be diluted to the oncocyte liquid of 1: 4 concentration with sterile saline, all mice right fore armpit subcutaneous vaccination 0.2ml/ Mus.After 24h, grouping is weighed, get Kunming mouse 40, male and female half and half, 18 ~ 22g, be divided into 4 groups at random, often organize 10, if normal saline group, cyclophosphamide (CTX) group, Rubidate group, medicine group of the present invention, the equal gastric infusion 10d of mice, except normal saline group, all the other each group all starts while gavage gives medicinal liquid, each group intraperitoneal injection of cyclophosphamide (CTX) 100mg/kg every day, continuous 3d, 24h after last administration, mice takes off cervical vertebra and puts to death, and peels off sarcoma, scales/electronic balance weighing.Calculate each group of mouse tumor galassing average and tumour inhibiting rate.(the average tumor of tumour inhibiting rate %=matched group average tumor weight-experimental group average tumor weight/matched group heavy × 100%).The results are shown in Table 3.
Table 3 pair cyclophosphamide suppresses murine sarcoma S
180the impact (x ± s, n=10) of tumor weight and tumour inhibiting rate
Note: compare after treating with normal group,
■ ■ ■p<0.001,
■ ■p<0.01,
■p<0.05; Cyclophosphamide group * * P<0.01, * P<0.05.
As shown in Table 3, compare with normal saline group, medicine group of the present invention and Rubidate group are to murine sarcoma S
180tumor weight average has suppression in various degree and reaches significance, and wherein, medicine group inhibitory rate 53.38% of the present invention is better than Rubidate group.
The Attenuation of 2.2 pairs of tumor-bearing mice chemotherapy
Get abdominal part inoculation S
180the tumor-bearing mice of tumor strain, extracts abdominal cavity tumor liquid under sterile working, be diluted to the oncocyte liquid of 1: 4 concentration with sterile saline, all mice right fore armpit subcutaneous vaccination 0.2ml/ Mus., test establishes normal saline group, cyclophosphamide model group, Rubidate group (100mg/kg), medicine group of the present invention, rear 3 groups of mice successive administration 10d, and within the 7th day, gives cyclophosphamide (100mg/kg), continuous 3d in administration.After last is to cyclophosphamide the 3rd day, each group mouse orbit got blood, and get spleen and side whole femur, measure leukocyte count in blood respectively, index and spleen index and mouse bone marrow cells DNA content, the results are shown in Table 4.
The Attenuation (x ± s, n=10) of table 4 pair tumor-bearing mice chemotherapy
Note: compare after treating with normal group,
■p<0.05,
■ ■ ■p<0.001; Compare with cyclophosphamide group, * * P<0.01, * * * P<0.001.
From experimental result table 4, compare with normal saline group, the mice of cyclophosphamide model group all has obvious reduction in index and spleen index and white blood cell parameters index, and reaches the diversity (P value <0.05,0.001) on statistical significance, and this illustrates this experiment modeling success; The test mice of medicine group of the present invention compares with cyclophosphamide model group in leukocyte number, there is larger rising and reach significant diversity (P < 0.001, P < 0.01), and for index and spleen index, though do not reach meaning statistically, also there is good effect trend.It can thus be appreciated that medicine group of the present invention can improve the drug side effect caused by chemotherapy preferably.
2.3 pairs of mices
60the protective effect of Co-r roentgenization
Kunming mouse 48, often organize 12, being divided into 4 groups at random, is normal saline group, cyclophosphamide model group, Rubidate matched group, medicine group of the present invention respectively, rear 3 groups of mice warps
60co-r single fraction irradiation, exposure dose is 7.5Gy (it is 400R/min that rate is entered in agent).After each group of mice successive administration 7d, 4 groups of mouse weights, eye socket gets blood, wins spleen, thymus and side whole femur, calculates index and spleen index respectively, measures leukocyte count and mouse bone marrow cells DNA content in blood, the results are shown in Table 5.
The Attenuation (x ± s, n=10) of table 5 pair tumor animal radiotherapy
Note: compare after treating with normal group,
■ ■ ■p<0.001; Compare with cyclophosphamide group, * * P<0.01, * * * P<0.001.
As shown in Table 5: cyclophosphamide model group is compared with normal saline group, the quantity of leucocyte of mice, index and spleen index and DNA content numerical value reduce all significantly, and reach extremely significant statistical significance (P < 0.001), this illustrates this modeling success tested; Compare with cyclophosphamide group, medicine group of the present invention all significantly can raise the quantity of leucocyte of mice, index and spleen index and DNA content numerical value, and obtains significant difference (P < 0.001, P<0.01); The indices of the mice after medicine group treatment of the present invention compares with Rubidate group, has significantly more excellent effect.In a word, this experimental study discloses: medicine group of the present invention has comparatively significantly Attenuation for the every important physiological parameter of the mice after radiotherapy.
Shown by above 3 groups of result of the tests, the tumor weight of the tumor-bearing mice that medicine of the present invention can obviously suppress, leukocyte increasing number and increase index and spleen index, and can significantly improve
60co-r irradiates mouse spleen index and bone marrow nucleated cell declines, and this experimental result is pointed out: medicine of the present invention has obvious Attenuation to change, radiotherapy, and itself and cyclophosphamide share has obvious synergism.
Detailed description of the invention
Below the specific embodiment of content of the present invention, for set forth in present specification want the technical scheme of technical solution problem, contributes to those skilled in the art and understand content of the present invention, but the realization of technical solution of the present invention is not limited to these embodiments.
Embodiment 1
(1), by Polyporus 150g, Poria 100g, is ground into fine powder, for subsequent use;
(2) get Radix Ginseng Rubra medical material 50g, be ground into coarse powder, by medical material weight ratio 8 times amount, concentration be 70% ethanol carry out seepage pressure effects, collect percolate and also reclaim ethanol, concentrate drying is also ground into dried cream powder, and for subsequent use, medicinal residues are continued to employ;
By step medicinal residues (2) again with Radix Astragali 100g, Fructus Lycii 100g, Fructus Ligustri Lucidi 100g, add the soak by water 2 times that medical material weight ratio is 9 times amount, each 1.5h, filter, merging filtrate, concentrating under reduced pressure become relative density be 1.10 clear paste, add ethanol to alcohol content 70%, cold preservation leaves standstill 24h, filters, filtrate recycling ethanol and through concentrating under reduced pressure become relative density be 1.20 clear paste, add step (1) in the medicated powder of 2/3 amount, mixing, is dryly also ground into dried cream powder, sieve for subsequent use;
(4) get above-mentioned steps (1) in remaining medicated powder, and step (2), (3) in dried cream powder, fully after mixing, obtain Traditional Chinese drug mixture, the substrate hot melt with suitable, stirs, and carries out dripping, make 1000g drop pill.Embodiment 2
(1), by Polyporus 150g, Poria 100g, is ground into fine powder, for subsequent use;
(2) get Radix Ginseng Rubra medical material 50g, be ground into coarse powder, by medical material weight ratio 8 times amount, concentration be 60% ethanol carry out seepage pressure effects, collect percolate and also reclaim ethanol, concentrate drying is also ground into dried cream powder, and for subsequent use, medicinal residues are continued to employ;
By step medicinal residues (2) again with Radix Astragali 100g, Fructus Lycii 100g, Fructus Ligustri Lucidi 100g, medical material merges, and adds the soak by water 2 times that medical material weight ratio is 10 times amount, each 1h, filters, merging filtrate, concentrating under reduced pressure become relative density be 1.10 clear paste, add ethanol to alcohol content 50%, cold preservation leaves standstill 12h, filter, filtrate recycling ethanol and through concentrating under reduced pressure become relative density be 1.20 clear paste, add step (1) in the medicated powder of 2/3 amount, mixing, drying is also ground into dried cream powder, sieves for subsequent use;
(4) get above-mentioned steps (1) in remaining medicated powder, and step (2), (3) in dried cream powder, fully after mixing, obtain Traditional Chinese drug mixture, with appropriate dextrin, sucrose, mixing, granulates, dry, makes 1000g granule.Embodiment 3
(1), by Polyporus 150g, Poria 100g, is ground into fine powder, for subsequent use;
(2) get Radix Ginseng Rubra medical material 50g, be ground into coarse powder, use 10 times amount, concentration be 80% ethanol carry out seepage pressure effects, collect percolate and also reclaim ethanol, concentrate drying is also ground into dried cream powder, and for subsequent use, medicinal residues are continued to employ;
By step medicinal residues (2) again with Radix Astragali 100g, Fructus Lycii 100g, Fructus Ligustri Lucidi 100g, medical material merges, and adds the soak by water 3 times that medical material weight ratio is 8 times amount, each 2h, filters, merging filtrate, concentrating under reduced pressure become relative density be 1.10 clear paste, add ethanol to alcohol content 60%, cold preservation leaves standstill 36h, filter, filtrate recycling ethanol and through concentrating under reduced pressure become relative density be 1.20 clear paste, add step (1) in the medicated powder of 2/3 amount, mixing, vacuum drying becomes dry cream, and is ground into dried cream powder, sieve for subsequent use;
(4) get above-mentioned steps (1) in remaining medicated powder, and step (2), (3) in dried cream powder, fully after mixing, obtain Traditional Chinese drug mixture, mix with suitable amount of sucrose, sodium benzoate, add water and make 1000ml oral solutions.
Embodiment 4
(1), by Polyporus 150g, Poria 100g, is ground into fine powder, for subsequent use;
(2) get Radix Ginseng Rubra medical material 50g, be ground into coarse powder, use 7 times amount, concentration be 50% ethanol carry out seepage pressure effects, collect percolate and also reclaim ethanol, concentrate drying is also ground into dried cream powder, and for subsequent use, medicinal residues are continued to employ;
By step medicinal residues (2) again with Radix Astragali 100g, Fructus Lycii 100g, Fructus Ligustri Lucidi 100g, medical material merges, add the soak by water 1 time that medical material weight ratio is 11 times amount, each 1h, filter, merging filtrate, concentrating under reduced pressure become relative density be 1.10 clear paste, add ethanol to alcohol content 80%, cold preservation leaves standstill 24h, filter, filtrate recycling ethanol and through concentrating under reduced pressure become relative density be 1.20 clear paste, add step (1) in the medicated powder of 2/3 amount, mixing, vacuum drying becomes dry cream, and is ground into dried cream powder, sieve for subsequent use;
(4) get above-mentioned steps (1) in remaining medicated powder, and step (2), (3) in dried cream powder, fully after mixing, obtain Traditional Chinese drug mixture, add suitable adjuvant, granulate, encapsulated, make 1000 hard capsules.
Claims (5)
1. treat a Chinese medicine preparation for oncosis, it is made up of the crude drug of following weight proportion: Radix Ginseng Rubra 50 parts, the Radix Astragali 100 parts, Fructus Lycii 100 parts, Fructus Ligustri Lucidi 100 parts, Polyporus 150 parts, 100 parts, Poria; It is characterized in that, this Chinese medicine preparation is prepared from by following technique:
(1) Polyporus, Poria powder are broken into fine powder, for subsequent use;
(2) get Radix Ginseng Rubra medical material, be ground into coarse powder, by medical material weight ratio 7 ~ 10 times amount, concentration be 50% ~ 80% ethanol carry out seepage pressure effects, collect percolate and also reclaim ethanol, concentrate drying is also ground into dried cream powder, and for subsequent use, medicinal residues are continued to employ;
By step (2) in medicinal residues merge with the Radix Astragali, Fructus Lycii, Fructus Ligustri Lucidi again, add the soak by water 1 ~ 3 time that medical material weight ratio is 8 ~ 11 times amount, each 1 ~ 2h, filter, merging filtrate, concentrating under reduced pressure become relative density be 1.10 clear paste, add ethanol to alcohol content 50% ~ 80%, cold preservation leaves standstill 12 ~ 36h, filters, filtrate recycling ethanol and through concentrating under reduced pressure become relative density be 1.20 clear paste, add step (1) in the medicated powder for subsequent use of 2/3 amount, mixing, is dryly also ground into dried cream powder, sieve for subsequent use;
(4) get above-mentioned steps (1) in medicated powder remaining for subsequent use, and step (2), (3) in dried cream powder, fully after mixing, obtain Traditional Chinese drug mixture, then add adjuvant conventional on pharmaceutics and make various different oral formulations, to obtain final product.
2. Chinese medicine preparation as claimed in claim 1, it is characterized in that, it is prepared from by following technique:
(1) Polyporus, Poria powder are broken into fine powder, for subsequent use;
(2) get Radix Ginseng Rubra medical material, be ground into coarse powder, by medical material weight ratio 8 times amount, concentration be 70% ethanol carry out seepage pressure effects, collect percolate and also reclaim ethanol, concentrate drying is also ground into dried cream powder, and for subsequent use, medicinal residues are continued to employ;
By step (2) in medicinal residues merge with the Radix Astragali, Fructus Lycii, Fructus Ligustri Lucidi again, add the soak by water 2 times that medical material weight ratio is 9 times amount, each 1.5h, filter, merging filtrate, concentrating under reduced pressure become relative density be 1.10 clear paste, add ethanol to alcohol content 70%, cold preservation leaves standstill 24h, filters, filtrate recycling ethanol and through concentrating under reduced pressure become relative density be 1.20 clear paste, add step (1) in the medicated powder for subsequent use of 2/3 amount, mixing, is dryly also ground into dried cream powder, sieve for subsequent use;
(4) get above-mentioned steps (1) in remaining medicated powder for subsequent use, and step (2), (3) in dried cream powder, fully after mixing, obtain Traditional Chinese drug mixture, then add adjuvant conventional on pharmaceutics and make various different oral formulations, to obtain final product.
3. Chinese medicine preparation as claimed in claim 1 or 2, is characterized in that this Chinese medicine preparation is oral solutions, granule, hard capsule or drop pill.
4. prepare the method for Chinese medicine preparation as claimed in claim 1, it is characterized in that, operate as follows:
(1) Polyporus, Poria powder are broken into fine powder, for subsequent use;
(2) get Radix Ginseng Rubra medical material, be ground into coarse powder, by medical material weight ratio 7 ~ 10 times amount, concentration be 50% ~ 80% ethanol carry out seepage pressure effects, collect percolate and also reclaim ethanol, concentrate drying is also ground into dried cream powder, and for subsequent use, medicinal residues are continued to employ;
By step (2) in medicinal residues merge with the Radix Astragali, Fructus Lycii, Fructus Ligustri Lucidi again, add the soak by water 1 ~ 3 time that medical material weight ratio is 8 ~ 11 times amount, each 1 ~ 2h, filter, merging filtrate, concentrating under reduced pressure become relative density be 1.10 clear paste, add ethanol to alcohol content 50% ~ 80%, cold preservation leaves standstill 12 ~ 36h, filters, filtrate recycling ethanol and through concentrating under reduced pressure become relative density be 1.20 clear paste, add step (1) in the medicated powder for subsequent use of 2/3 amount, mixing, is dryly also ground into dried cream powder, sieve for subsequent use;
(4) get above-mentioned steps (1) in medicated powder remaining for subsequent use, and step (2), (3) in dried cream powder, fully after mixing, obtain Traditional Chinese drug mixture, then add adjuvant conventional on pharmaceutics and make various different oral formulations, to obtain final product.
5. the preparation method of Chinese medicine preparation as claimed in claim 4, is characterized in that:
(1) Polyporus, Poria powder are broken into fine powder, for subsequent use;
(2) get Radix Ginseng Rubra medical material, be ground into coarse powder, by medical material weight ratio 8 times amount, concentration be 70% ethanol carry out seepage pressure effects, collect percolate and also reclaim ethanol, concentrate drying is also ground into dried cream powder, and for subsequent use, medicinal residues are continued to employ;
By step (2) in medicinal residues merge with the Radix Astragali, Fructus Lycii, Fructus Ligustri Lucidi again, add the soak by water 2 times that medical material weight ratio is 9 times amount, each 1.5h, filter, merging filtrate, concentrating under reduced pressure become relative density be 1.10 clear paste, add ethanol to alcohol content 70%, cold preservation leaves standstill 24h, filters, filtrate recycling ethanol and through concentrating under reduced pressure become relative density be 1.20 clear paste, add step (1) in the medicated powder for subsequent use of 2/3 amount, mixing, is dryly also ground into dried cream powder, sieve for subsequent use;
(4) get above-mentioned steps (1) in remaining medicated powder for subsequent use, and step (2), (3) in dried cream powder, fully after mixing, obtain Traditional Chinese drug mixture, then add adjuvant conventional on pharmaceutics and make various different oral formulations, to obtain final product.
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| CN1544078A (en) * | 2003-09-25 | 2004-11-10 | 咸阳步长医药科技发展有限公司 | Chinese patent medicine for treating leukopenia due to tumor treating radiotherapy and chemotherapy |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1544078A (en) * | 2003-09-25 | 2004-11-10 | 咸阳步长医药科技发展有限公司 | Chinese patent medicine for treating leukopenia due to tumor treating radiotherapy and chemotherapy |
Non-Patent Citations (2)
| Title |
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| 何昌国等: "复方槟榔灌肠液的制备及临床应用", 《中国中医药科技》 * |
| 向丽华等: "五苓散对腹泻模型大鼠小肠绒毛高度、宽度的影响", 《中国中医基础医学杂志》 * |
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