CN104738314B - Chinese herbal microecological preparation of the anti-transport stress of Penaeus Vannmei and its preparation method and application - Google Patents
Chinese herbal microecological preparation of the anti-transport stress of Penaeus Vannmei and its preparation method and application Download PDFInfo
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Abstract
The present invention relates to a kind of Chinese herbal microecological preparation in Penaeus Vannmei moderate resistance transport stress, the raw material comprising following parts by weight is made：22~26 parts of Chinese gall, 14~21 parts of poplar flower, 5~20 parts of felwort, 10~15 parts of scutellaria, 5~10 parts of cortex acanthopanacis and fermentation bacterial strain and fluid nutrient medium.Said preparation is by the way that liquid state fermentation in the fermented liquid medium within bacterial strain of herbal raw material is obtained.Beneficial effects of the present invention are as follows：1st, composite Chinese herbal raw material components wide material sources, with low cost, preparation are simply and effect is notable；2nd, fermentation strain is that possess decomposition, produce the beneficial bacterial strain of polyphenoils, can largely discharge the active component of Chinese herbal medicine, and have some improvement for prawn disease control and intestinal health；3rd, can significantly reduce occur in prawn transportation stress corpus hemorrhagicum phenomenon；4th, applied in Penaeus Vannmei, substantially, the survival rate of Penaeus Vannmei transport is significantly improved economic value.
Description
Technical field
The present invention relates to a kind of composite Chinese herbal probiotics of resisting stress, and in particular to a kind of anti-in Penaeus Vannmei
Chinese herbal microecological preparation of transport stress and its preparation method and application.
Background technology
Penaeus Vannmei also known as Environment of Litopenaeus vannamei Low, are commonly called as white shrimp, Penaeus Vannmei compared with other domestic cultured prawns,
Have the advantages that growth is fast, resistance against diseases is strong, it is wide to adapt to salinity, be the big kind of world today's shrimps in culture yield highest three
One of (Chinese prawn, Penaeus monodon, Penaeus Vannmei).With the lifting of people's living standard, prawn is nutritious due to its,
Meat is soft, the features such as easy to digest, is well received by consumers.
In order to improve the yield of prawn culturing, existing prawn culturing using intensive culture, transport and is sold.Existing
Have under high yielding culture pattern, the change of prawn environment to external world is very sensitive, climate change, cultivation operation, the use of medicine, adopt
What the excessive grade of appetite can produce its prawn in varying degrees stress." stress " it is a kind of common physiology shape of aquatic livestock
State, this is the total of the various physiological stresses made when aquatic livestock body produces abnormal stimulation in environment or body to external world
With.Prawn in stress situation is by every physiological action, and each organ for transferring body carrys out the mutation of response environment." should
Swash " substantial amounts of physical efficiency is consumed, physical stamina letdown, immunocompetence will decline, and easily cause prawn to occur disease.
In prawn transportation, the water quality factor such as dissolved oxygen, salinity, temperature, acid-base value in prawn transport water body
Drastically the undesired water such as change easily induces the stress reaction of prawn, in addition, due to supporting during prawn is transported and sells
Grow density much, can make prawn generation is crowded stress.
In high density prawn transportation, aquaculture is also turned into for that stress be lost caused by the change of the factors such as environment
, not yet there is the biological agent for being directed to prawn transport stress at present on the market in the problem of industry is very important, therefore exploitation is a kind of
Natural, the free of contamination biological agent for being directed to Penaeus Vannmei transport is extremely important, the safety for promoting prawn transport
Property, lifting prawn transportation survival rate it is significant.
The content of the invention
Not enough for more than, an object of the present invention is to provide one kind specifically designed for Penaeus Vannmei transport stress
, it is possible to increase Penaeus Vannmei transports anti-stress ability, so as to improve the composite Chinese herbal probiotics of transportation survival rate.
The present invention reaches above-mentioned purpose by following scheme：
The Chinese herbal microecological preparation of the present invention is by biofermentation technique, by microorganism and Chinese herbal medicine mixed fermentation system
Into being a kind of full constituent zymotic fluid containing effective Chinese herbal medicine active component, thalline and metabolite.
A kind of Chinese herbal microecological preparation of the anti-transport stress of Penaeus Vannmei, the raw material comprising following parts by weight is made：
22~26 parts of Chinese gall, preferably 22 parts, 14~21 parts of poplar flower, preferably 16 parts, 5~20 parts of felwort, preferably 18 parts, scutellaria 10
~15 parts, preferably 10 parts, 5~10 parts of cortex acanthopanacis, preferably 5 parts and fermentation bacterial strain and fluid nutrient medium.
A kind of Chinese herbal microecological preparation of the anti-transport stress of preferred Penaeus Vannmei, includes the raw material of following parts by weight
It is made：22 parts of Chinese gall, 16 parts of poplar flower, 18 parts of felwort, 10 parts of scutellaria, 5 parts of cortex acanthopanacis and fermentation bacterial strain and liquid are trained
Support base.
Wherein, above-mentioned fermentation preferably comprises bacillus and photosynthetic bacteria with bacterial strain, is confirmed through research, and bacillus possesses
The characteristic of High Cellulase Production, and have good inhibiting effect for the common vibrio parahaemolytious of prawn；And photosynthetic bacteria enters one
Step is preferred to use Rhodopseudomonas spheroides, and its metabolite enriches, and said herbal medicine grows to it and has obvious rush in the present invention
Enter effect, the presence of the bacterial strain has certain promotion to make for the lifting of bacillus bacterium number and the stabilization of zymotic fluid bacterium number
With.It can be acted synergistically with the formation of the active ingredient in said herbal medicine raw material components in the present invention, while the bacterial strain can also have
Different oxide growth is carried out under oxygen environment.
Wherein, the fluid nutrient medium can be any culture available for said herbal medicine probiotics fermented and cultured
Base, preferably liquid molasses, more preferably percent by volume are 10-20% liquid molasses, preferably percent by volume
For 10% liquid molasses, remaining is water, wherein, the pH of aforesaid liquid molasses culture medium is more preferably 7.0.
Wherein, said herbal medicine raw material is preferably powdery, such as after crushing, more preferably by ultramicro grinding
Powdery afterwards.
Wherein, above-mentioned powdery herbal raw material accounts for the percent by volume preferably 15-25% of the volume of fluid nutrient medium.
Wherein, the percent by volume that the inoculum concentration of above-mentioned bacillus preferably accounts for fluid nutrient medium volume is 2-3%, more excellent
Elect 2% as, viable count is preferably 2 × 109-1×1010Cfu/ml, more preferably 2 × 109cfu/ml。
Wherein, the percent by volume that above-mentioned photosynthetic bacteria inoculum concentration preferably accounts for fluid nutrient medium volume is 4-5%, more preferably
For 5%, viable count is preferably 3 × 108-5×108Cfu/ml, more preferably 3 × 108cfu/ml。
Wherein, above-mentioned photosynthetic bacteria can be Rhodopseudomonas palustris, capsula Rhodopseudomonas, Rhodopseudomonas spheroides,
Preferably capsula Rhodopseudomonas, more preferably Rhodopseudomonas spheroides, Rhodopseudomonas spheroides.
Wherein, said herbal medicine probiotics viable count or cell number preferably >=2 × 107cfu/ml。
The second object of the present invention is to provide a kind of Chinese herbal medicine micro-ecological system of the above-mentioned anti-transport stress of Penaeus Vannmei
The preparation method of agent, including by liquid state fermentation in the fermented liquid medium within bacterial strain of herbal raw material prepare it is above-mentioned in
Herbal medicine probiotics, wherein, the selection of raw material components and consumption are from including the above-mentioned anti-fortune of all Penaeus Vannmeis referred to
It is defeated stress Chinese herbal microecological preparation in raw material.
Further, a kind of side of the preferred Chinese herbal microecological preparation for preparing the anti-transport stress of above-mentioned Penaeus Vannmei
Method, including：By herbal raw material after crushed, preferably after ultramicro grinding to account for fluid nutrient medium percent by volume 15%-25%
Amount be added in fluid nutrient medium, inoculation fermentation bacterial strain, fermentation is obtained after fermentation ends, further can be with filling or fill
Seal more preferable after dress, wherein, the selection of raw material components and consumption are from including the above-mentioned anti-transport of all Penaeus Vannmeis referred to
Stress Chinese herbal microecological preparation in raw material.
Wherein, it is 30-35 DEG C, throughput 10-20Lmin that the parameter of above-mentioned fermentation, which is preferably controlled to fermentation temperature,-1, revolution：
180-200r·min-1, fermentation time 24-36h.
Wherein, above-mentioned preparation method preferably controls the viable count or cell number of the Chinese herbal microecological preparation prepared
≥2×107cfu/ml。
Further, a kind of Chinese herbal microecological preparation for more preferably preparing the anti-transport stress of above-mentioned Penaeus Vannmei
Method, comprise the following steps：
(1) herbal raw material ultramicro grinding is obtained into herbal raw material pulvis, preferably 80% crosses 100 mesh sieves or crosses 200 mesh
Sieve obtains herbal raw material pulvis；
(2) fluid nutrient medium is prepared, the fluid nutrient medium includes water and liquid molasses, wherein, to account for fluid nutrient medium
Volume percentage, liquid molasses are 10-20-%, preferably 10%；
(3) herbal raw material pulvis is trained with the amount for accounting for fluid nutrient medium percent by volume 15%-25% added to liquid
Support in base, inoculation fermentation bacterial strain, fermentation temperature is 30-35 DEG C, throughput 10-20Lmin-1, revolution：180-200r·min-1, fermentation time 24-36h；
(4) fermentation post processing：After fermentation ends, the mixture of thalline, Chinese herbal medicine fermented liquid, i.e. Chinese herbal medicine micro-ecological are obtained
Preparation, it is filling after further stirring or filling and sealing is more preferably obtained after stirring, the viable count or cell of the preparation
Number >=2 × 107Cfu/ml, wherein, the selection of raw material components and consumption are resisted from the above-mentioned all Penaeus Vannmeis referred to are included
Raw material in the Chinese herbal microecological preparation of transport stress..
The three of the goal of the invention of the present invention are to provide a kind of Chinese herbal medicine of the above-mentioned anti-transport stress of Penaeus Vannmei of application
The method of probiotics, including said herbal medicine probiotics is used for before transporting and/or in transportation, preferably into shrimp
Used for three days on end before receiving shrimp, 1 time a day, each consumption 1L/ mus of rice-1；Or preferably young shrimp or into using in shrimp transportation, often
Secondary consumption 50ml/m3Water body, by such application, can effectively slow down reaction of the body to stressed condition, lift cultivated animals
Tolerance in transportation, significantly reduce because transportation stress caused by injures and deaths.
Herb fermenting preparation technique be inherit science of Chinese drug processing fermentation method on the basis of, absorb Microecology into
Really, the Chinese medicine new technology with reference to formed by the fermentation technique of modern biological project, is by active ingredient of Chinese herbs, thalline and metabolism
The novel fermentation preparation combined of product, a few days ago in the multi-field focus as research such as pharmacy.The present invention's is used for south
The fermentation of Chinese herbal medicine probiotics of the anti-transport stress of penaeus vannamei, be exactly using modern biotechnology fermentation technique, by microorganism with
Chinese herbal medicine mixed fermentation is made, and is a kind of full constituent zymotic fluid containing effective active ingredient of Chinese herbs, thalline and metabolite, examination
Checking is real, can effectively alleviate prawn in transportation stress, tolerance of the lifting cultivated animals in body was transported is reduced
The injures and deaths of prawn transport, improve the transportation survival rate of Penaeus Vannmei, with significant economic value.
Compared with prior art, the invention has the advantages that：
(1) present invention from shrimp body stress angle, screened a variety of resisting stress, anti-microbial pathogen Chinese herbal medicine, what is obtained is compound
Herbal raw material Component Source is extensive, with low cost, prepare simple and effect is notable；
(2) fermentation strain that uses of the present invention, be screened for many years through this laboratory obtain possess decomposition, secrete anti-oxidant
The beneficial bacterial strain of material, can not only largely discharge the active component of Chinese herbal medicine, while bacterial strain is in itself for prawn disease control and intestines
Road health has some improvement；
(3) anti-transport stress fermented tcm preparation of the present invention is specific to form in Penaeus Vannmei exploitation,
The use of the probiotics can significantly reduce occur in prawn transportation stress corpus hemorrhagicum phenomenon.
(4) substantially, the survival rate of Penaeus Vannmei transport is significantly improved economic value.
Embodiment
The present invention is further described below in conjunction with specific embodiment.
Embodiment 1：
A kind of Chinese herbal microecological preparation of the anti-transport stress of Penaeus Vannmei, the raw material comprising following parts by weight is made：
22~26 parts of Chinese gall, 14~21 parts of poplar flower, 5~20 parts of felwort, 10~15 parts of scutellaria, 5~10 parts of cortex acanthopanacis, Yi Jifa
Ferment bacterial strain and fluid nutrient medium.
Embodiment 2：
A kind of Chinese herbal microecological preparation of the anti-transport stress of Penaeus Vannmei, the raw material comprising following parts by weight is made：
22~26 parts of Chinese gall, 14~21 parts of poplar flower, 5~20 parts of felwort, 10~15 parts of scutellaria, 5~10 parts of cortex acanthopanacis, Yi Jiya
Spore bacillus, photosynthetic bacteria and liquid molasses culture medium.
Embodiment 3：
A kind of Chinese herbal microecological preparation of the anti-transport stress of Penaeus Vannmei, the raw material comprising following parts by weight is made：
22~26 parts of Chinese gall, 14~21 parts of poplar flower, 5~20 parts of felwort, 10~15 parts of scutellaria, 5~10 parts of cortex acanthopanacis, Yi Jiya
Powdery herbal raw material component after spore bacillus, photosynthetic bacteria and liquid molasses culture medium, herbal raw material ultramicro grinding, the powder
The percent by volume that shape component accounts for liquid molasses culture volume is 15-25%, and the inoculum concentration of bacillus accounts for the training of liquid molasses
The percent by volume for supporting matrix product is 2-3%, and viable count is 2 × 109-1×1010Cfu/ml, above-mentioned photosynthetic bacteria inoculum concentration is accounted for
The percent by volume of fluid nutrient medium volume is 4-5%, and viable count is 3 × 108-5×108Cfu/ml, liquid molasses culture medium by
Water and liquid molasses composition, wherein liquid molasses account for the 10%-20% of liquid molasses culture volume.
Embodiment 4：
A kind of viable count or thin in the Chinese herbal microecological preparation of the anti-transport stress of Penaeus Vannmei, the probiotics
Born of the same parents number >=2 × 107Cfu/ml, is made up of the raw material of following parts by weight：22~26 parts of Chinese gall, 14~21 parts of poplar flower, rough gentian
5~20 parts of grass, 10~15 parts of scutellaria, 5~10 parts of cortex acanthopanacis, and bacillus, photosynthetic bacteria and liquid molasses culture medium,
Powdery herbal raw material component after herbal raw material ultramicro grinding, the powdered components account for the volume of liquid molasses culture volume
Percentage is 20%, and the inoculum concentration of bacillus accounts for the percent by volume of liquid molasses culture volume for 2-3%, and viable count is
2×109-1×1010Cfu/ml, the percent by volume that above-mentioned photosynthetic bacteria inoculum concentration accounts for fluid nutrient medium volume is 4-5%, living
Bacterium number is 3 × 108-5×108Cfu/ml, liquid molasses culture medium is made up of water and liquid molasses, and wherein liquid molasses account for liquid
The 10% of molasses culture medium volume.
Embodiment 5：
A kind of Chinese herbal microecological preparation of the anti-transport stress of Penaeus Vannmei, the raw material comprising following parts by weight is made：
24 parts of Chinese gall, 21 parts of poplar flower, 12 parts of felwort, 12 parts of scutellaria, 8 parts of cortex acanthopanacis and fermentation bacterial strain and fluid nutrient medium.
Embodiment 6：
A kind of Chinese herbal microecological preparation of the anti-transport stress of Penaeus Vannmei, is made up of the raw material of following parts by weight：Five
26 parts of gall nut, 18 parts of poplar flower, 20 parts of felwort, 11 parts of scutellaria, 6.5 parts of cortex acanthopanacis and bacillus, photosynthetic bacteria and liquid
Body molasses culture medium.
Embodiment 7：
A kind of Chinese herbal microecological preparation of the anti-transport stress of Penaeus Vannmei, the raw material comprising following parts by weight is made：
22 parts of Chinese gall, 14 parts of poplar flower, 8 parts of felwort, 15 parts of scutellaria, 10 parts of cortex acanthopanacis and bacillus, spherical red false unit cell
Powdery herbal raw material component after bacterium (photosynthetic bacteria) and liquid molasses culture medium, herbal raw material ultramicro grinding, the powdery
The percent by volume that component accounts for liquid molasses culture volume is 15-25%, and the inoculum concentration of bacillus accounts for liquid molasses culture
The percent by volume of matrix product is 2-3%, and viable count is 2 × 109-1×1010Cfu/ml, above-mentioned Rhodopseudomonas spheroides is (photosynthetic
Bacterium) inoculum concentration account for fluid nutrient medium volume percent by volume be 4-5%, viable count be 3 × 108-5×108Cfu/ml, liquid
Body molasses culture medium is made up of water and liquid molasses, and wherein liquid molasses account for the 8%-12% of liquid molasses culture volume.
Embodiment 8：
A kind of viable count or thin in the Chinese herbal microecological preparation of the anti-transport stress of Penaeus Vannmei, the probiotics
Born of the same parents number >=2 × 107Cfu/ml, is made up of the raw material of following parts by weight：22 parts of Chinese gall, 16 parts of poplar flower, 18 parts of felwort is yellow
10 parts of Cen, 5 parts of cortex acanthopanacis, and bacillus, Rhodopseudomonas spheroides (photosynthetic bacteria) and liquid molasses culture medium, Chinese herbal medicine
Powdery herbal raw material component after raw material ultramicro grinding, the powdered components account for the percent by volume of liquid molasses culture volume
For 20%, the percent by volume that the inoculum concentration of bacillus accounts for liquid molasses culture volume is 2%, viable count is 2 ×
109Cfu/ml, the percent by volume that above-mentioned Rhodopseudomonas spheroides (photosynthetic bacteria) inoculum concentration accounts for fluid nutrient medium volume is
5%, viable count is 3 × 108Cfu/ml, liquid molasses culture medium is made up of water and liquid molasses, and wherein liquid molasses account for liquid
The 10% of molasses culture medium volume.
Embodiment 9
A kind of preparation method of the Chinese herbal microecological preparation of the anti-transport stress of above-mentioned Penaeus Vannmei, by herbal raw material
Liquid state fermentation prepares said herbal medicine probiotics in the fermented liquid medium within bacterial strain, wherein, said process
In each raw material components selection and consumption be respectively embodiment 1-8.
Embodiment 10
A kind of method for the Chinese herbal microecological preparation for preparing the anti-transport stress of above-mentioned Penaeus Vannmei, including：By medium-height grass
Medicine raw material after crushed, is added in fluid nutrient medium with the amount for accounting for fluid nutrient medium percent by volume 15%-25%, inoculation hair
Filling and sealing is to prepare Chinese herbal microecological preparation after ferment bacterial strain, fermentation, fermentation ends, wherein, it is each in said process
The selection of raw material components and consumption select respectively embodiment 1-8.
Embodiment 11
A kind of method for the Chinese herbal microecological preparation for preparing the anti-transport stress of above-mentioned Penaeus Vannmei, including：
(1) by herbal raw material after crushed, liquid is added to the amount for accounting for fluid nutrient medium percent by volume 15%-25%
In body culture medium；
(2) inoculation fermentation bacterial strain, fermentation, fermentation temperature is 30-35 DEG C, throughput 10-20Lmin-1, revolution：180-
200r·min-1Fermentation time 24-36h；
(3) fermentation ends obtain Chinese herbal microecological preparation, and the viable count of Chinese herbal microecological preparation or cell number >=2 ×
107Cfu/ml, wherein, the selection of each raw material components and consumption select respectively embodiment 1-8 in above-mentioned steps.
Embodiment 12
A kind of method for the Chinese herbal microecological preparation for preparing the anti-transport stress of above-mentioned Penaeus Vannmei：
(1) herbal raw material ultramicro grinding is obtained into herbal raw material pulvis, 80%, which crosses 100 mesh sieves, obtains herbal raw material
Pulvis；
(2) fluid nutrient medium is prepared, fluid nutrient medium includes water and liquid molasses, wherein, to account for fluid nutrient medium volume
Percentages, liquid molasses are 10%；
(3) herbal raw material pulvis is trained with the amount for accounting for fluid nutrient medium percent by volume 15%-25% added to liquid
Support in base, inoculation fermentation bacterial strain, fermentation temperature is 32 DEG C, throughput 20Lmin-1, revolution：180r·min-1Fermentation time
32h；
(4) fermentation post processing：The filling or filling and sealing that stirred after fermentation ends obtains Chinese herbal microecological preparation, institute
State viable count or cell number >=2 × 10 of preparation7Cfu/ml, wherein, the selection of each raw material components and consumption choosing in above-mentioned steps
With respectively embodiment 1-8.
Embodiment 13
The Chinese herbal microecological preparation and embodiment 9- of a kind of anti-transport stress of Penaeus Vannmei in Application Example 1-8
The method of the Chinese herbal microecological preparation of the 12 anti-transport stresses of Penaeus Vannmei prepared, including by said herbal medicine Tiny ecosystem system
Agent is used for before transporting and/or in transportation.
Embodiment 14
The Chinese herbal microecological preparation and embodiment 9- of a kind of anti-transport stress of Penaeus Vannmei in Application Example 1-8
The method of the Chinese herbal microecological preparation of the 12 anti-transport stresses of Penaeus Vannmei prepared, said herbal medicine probiotics is used
Used for three days on end before shrimp is received into shrimp, 1 time a day, each consumption 1L/ mus of rice-1。
Embodiment 15
The Chinese herbal microecological preparation and embodiment 9- of a kind of anti-transport stress of Penaeus Vannmei in Application Example 1-8
The method of the Chinese herbal microecological preparation of the 12 anti-transport stresses of Penaeus Vannmei prepared, said herbal medicine probiotics is used
In young shrimp or into using in shrimp transportation, each consumption 50ml/m3Water body
The shrimp seedling of embodiment 16 transports effect experiment
Test material：It is right in vain with the South America that the raw material components of embodiment 8 are prepared using the preparation method of embodiment 12
The Chinese herbal microecological preparation of the anti-transport stress of shrimp, is used as sample 1.
Process of the test：Before shrimp seedling is emerged 2h be transferred to shrimp seedling bucket, then through shrimp seedling bucket by certain quantity be transferred to shrimp seedling bag
It is for sale, pool head is reached by transport, 2 above-mentioned resisting stress Chinese herbal microecological preparations (sample 1) is added altogether in the process and makees
For test group, added when shrimp seedling goes to shrimp seedling bucket, be once to add (to transport after shrimp seedling is transferred to shrimp seedling bag
Journey)；Meanwhile, control group is set, and without Chinese herbal microecological preparation in above-mentioned transfer process, experimental design is as shown in table 1.
Table 1：Experimental design table
| Project | Test group | Control group |
| Shrimp seedling specification | 0.5-0.6cm | 0.5-0.6cm |
| Shrimp seedling bucket | 1 barrel | 1 barrel |
| Fill seedling amount | Ten thousand tails of 250-300/bucket | Ten thousand tails of 250-300/bucket |
| Resisting stress fermentation of Chinese herbal medicine addition | 10ml/ barrels | Without |
| Fill seedling bag | 5 bags | 5 bags |
| Seedling bag specification | 36×18×54cm | 36×18×54cm |
| Fill seedling amount | Ten thousand tails of 1.8-2/bag | Ten thousand tails of 1.5-1.7/bag |
| Resisting stress fermentation of Chinese herbal medicine addition | 0.2ml/ bags | Without |
| Distance | Zhanjiang-Fanyu 5h | Zhanjiang-Fanyu 5h |
Result of the test：Test group is in the case where dress seedling amount is more than control group, and survival rate is higher than control group by 3.5%；Jejunum
For empty stomach ratio, control group 5-8% lower than test group；Ammonia nitrogen index experimental group exists in transport destination detection shrimp seedling bag
0.1-0.2mg/L, control group is slightly above test group about 0.4mg/L, and shrimp seedling enters after the pool, within 30min, and test group shrimp sinks to
Pool bottom, control group shrimp just sinks to pool bottom close to 2h, and the time for sinking to pool bottom has reacted shrimp seedling environment is fitted to a certain extent
Should be able to power, sink to that pool bottom time shorter shrimp vigor is better, adaptability is stronger.
As can be seen from the above test results, shrimp seedling transport before, transportation use resisting stress fermentation of Chinese herbal medicine preparation pair
In resistance transport stress, Lifting Convey survival rate has certain effect.
Embodiment 17 is into shrimp trafficking experiments
Test material：It is right in vain with the South America that the raw material components of embodiment 7 are prepared using the preparation method of embodiment 12
The Chinese herbal microecological preparation of the anti-transport stress of shrimp, is used as sample 2.
Process of the test：Prawn culturing is to 40-50 branch/jin, and 8 mu of ponds are typically disposably received 1000-1200 jins of shrimp, received before shrimp
The experiment pool uses resisting stress Chinese herbal microecological preparation (sample 2) 1 time a day within 3 days, each consumption 1L/ mus of rice-1, it is sub-packed in shrimp
In the cage of bucket, dress shrimp bucket uses resisting stress fermentation of Chinese herbal medicine dosage 50ml/m3Water body, while control group is designed, control group and examination
Test group differ only in without Chinese herbal microecological preparation, experimental design is as shown in table 2 and table 3.
Table 2：Experimental design table
| Project | Test group | Control group |
| Into shrimp specification | 40-50 branch/jin | 40-50 branch/jin |
| Fill shrimp bucket specification | 60×40×115cm | 60×40×115cm |
| Shrimp barreled water | 0.5m3Water | 0.5m3Water |
| Cage | 20 cage/buckets | 20 cage/buckets |
| Fill shrimp amount | 17-20 jins/cage | 17-20 jins/cage |
| Water source | Test pool water | Compare pool water |
| It is grouped (bucket) | 2 buckets | 2 buckets |
| Shrimp body gross weight | 300-400kg | 250-300kg |
| Haulage time | 4h | 4h |
Result of the test：
From the point of view of result of the test, test group shrimp transportation survival rate reaches 98-100%；Without substantially breaking, palpus, rubescent etc. are apparent to ask
Topic, shrimp vigor is good, sells lover；Control group shrimp survival rate 95% or so, and there have rubescent shrimp body, easy jump, disconnected palpus etc. to occur to be more.
The resisting stress Chinese herbal microecological preparation that it can be seen from two above experiment prepared by the present invention can effectively lift shrimp
Seedling and anti-stress ability is transported into shrimp, improve transportation survival rate, economic value is obvious.
Several photosynthetic bacterias of embodiment 18 and the synergy of bacillus
Test material：In addition to the addition of photosynthetic bacteria and bacillus is not in accordance with the addition of embodiment 8, remaining
The medium-height grass of the anti-transport stress of Penaeus Vannmei prepared with the raw material components of embodiment 8 using the preparation method of embodiment 12
Medicine probiotics, as zymotic fluid 3, it is other all, except：1st, spherical red false unit cell is replaced using Rhodopseudomonas palustris
Bacterium, is used as zymotic fluid 1；It is other all, except：2nd, capsula Rhodopseudomonas replace Rhodopseudomonas spheroides, are used as zymotic fluid
2；It is other all, except：3rd, without Rhodopseudomonas spheroides, water is added as blank control, zymotic fluid 4 is used as；Wherein,
In above-mentioned all zymotic fluids, the addition of three kinds of photosynthetic bacterias and bacillus is defined by tests below design.
Experimental design：In each group, above-mentioned three kinds of different photosynthetic bacterias are prepared into OD600 respectively and are approximately equal to 0.5 bacterium
Suspension (bacterium number about 2.1 × 108-3.6.×108Cfu/ml, is added to powdery Chinese herbal medicine former with the amount of percent by volume 4% respectively
Fermented in material, the water of same volume added in zymotic fluid 4, the addition of the bacillus in four kinds of zymotic fluids for 2 ×
109Cfu/ml, addition 2%, every group 3 are parallel.
Testing index：Photosynthetic bacteria bacterium number and gemma in four groups of zymotic fluids (i.e. four kinds Chinese herbal microecological preparation products)
Bacillus bacterium number, as shown in table 3.
Table 3：Photosynthetic bacteria bacterium number and bacillus bacterium number table in four kinds of zymotic fluids (Chinese herbal microecological preparation)
From the results shown in Table 3：The Chinese herbal microecological preparation of Rhodopseudomonas palustris fermentation, bacterium number is compared with other groups
It is relatively low, it can be determined that successively, the bacterial strain is slow-growing under this yeasting, Chinese herbal medicine is not clear to the bacterial strain growth promoting function
It is aobvious；For capsula Rhodopseudomonas, its growth has certain facilitation to Chinese herbal medicine with the strain of spherical Rhodopseudomonas, while also right
The growth of bacillus is also shown from the point of view of facilitation, the result of comprehensive zymotic fluid 4, and the addition of photosynthetic bacteria is for gemma
The growth of bacillus also has certain promotion, and the most obvious with the effect of Rhodopseudomonas spheroides (3 groups of zymotic fluid).
It is described above, it is only the preferably specific embodiment of the present invention, but protection scope of the present invention is not limited thereto,
Any one skilled in the art the invention discloses technical scope in, technique according to the invention scheme and its
Design is subject to equivalent substitution or change, should all cover within the scope of the present invention.
Claims (10)
1. a kind of Chinese herbal microecological preparation of the anti-transport stress of Penaeus Vannmei, it is characterised in that the Chinese herbal medicine micro-ecological
Preparation is made up of the raw material of following parts by weight：22~26 parts of Chinese gall, 14~21 parts of poplar flower, 5~20 parts of felwort, scutellaria 10
~15 parts, 5~10 parts of cortex acanthopanacis and fermentation bacterial strain and fluid nutrient medium.
2. a kind of Chinese herbal microecological preparation of the anti-transport stress of Penaeus Vannmei as claimed in claim 1, it is characterised in that
The fermentation includes bacillus, photosynthetic bacteria with bacterial strain.
3. a kind of Chinese herbal microecological preparation of the anti-transport stress of Penaeus Vannmei as claimed in claim 2, it is characterised in that
The photosynthetic bacteria is Rhodopseudomonas palustris, capsula Rhodopseudomonas, Rhodopseudomonas spheroides.
4. a kind of Chinese herbal microecological preparation of the anti-transport stress of Penaeus Vannmei as described in claim 1 or 3, its feature exists
In the fluid nutrient medium is the liquid molasses that percent by volume is 10-20%, and remaining is water, and pH is 7.0.
5. a kind of Chinese herbal microecological preparation of the anti-transport stress of Penaeus Vannmei as claimed in claim 1, it is characterised in that
The herbal raw material is powdery, and the herbal raw material of the powdery accounts for the percent by volume of fluid nutrient medium for 15-25%.
6. a kind of Chinese herbal microecological preparation of the anti-transport stress of Penaeus Vannmei as claimed in claim 4, it is characterised in that
The herbal raw material is powdery, and the herbal raw material of the powdery accounts for the percent by volume of fluid nutrient medium for 15-25%.
7. a kind of Chinese herbal microecological preparation of the anti-transport stress of Penaeus Vannmei as claimed in claim 2 or claim 3, its feature exists
In the inoculum concentration of the bacillus accounts for the percent by volume of fluid nutrient medium for 2-3%, and viable count is 2 × 109-1×
1010cfu/ml；And/or the photosynthetic bacteria inoculum concentration accounts for the percent by volume of fluid nutrient medium for 4-5%, viable count is 3-5
×108cfu/ml。
8. a kind of Chinese herbal medicine of the anti-transport stress of the Penaeus Vannmei prepared as described in any claim in claim 1-7 is micro-
The method of ecological agent, it is characterised in that prepare liquid state fermentation in the fermented use bacterial strain liquid medium within of herbal raw material
Obtain.
9. a kind of preparation method of the Chinese herbal microecological preparation of the anti-transport stress of Penaeus Vannmei as claimed in claim 8,
It is characterised in that it includes：It will be added after herbal raw material ultramicro grinding with the amount for accounting for fluid nutrient medium percent by volume 15%-25%
Add in fluid nutrient medium, inoculation fermentation bacterial strain, ferment, obtained after fermentation ends.
10. a kind of preparation method of the Chinese herbal microecological preparation of the anti-transport stress of Penaeus Vannmei as claimed in claim 9,
It is characterised in that it includes following steps：
(1) herbal raw material ultramicro grinding is obtained into herbal raw material pulvis；
(2) fluid nutrient medium is prepared, the fluid nutrient medium is made up of water and liquid molasses, wherein, it is described to account for Liquid Culture
Base volume percentage, liquid molasses are 10-20%；
(3) herbal raw material pulvis is added to fluid nutrient medium with the amount for accounting for fluid nutrient medium percent by volume 15%-25%
In, inoculation fermentation bacterial strain, fermentation temperature is 30-35 DEG C, throughput 10-20Lmin-1, revolution：180-200r·min-1,
Fermentation time 24-36h；
(4) fermentation post processing：After fermentation ends, be uniformly mixing to obtain Chinese herbal microecological preparation, the viable count of the preparation or
Cell number >=2 × 107cfu/ml。
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| CN107865267A (en) * | 2017-11-27 | 2018-04-03 | 陈程 | A kind of preparation method of penaeus vannamei boone feed additive |
| CN107788211A (en) * | 2017-11-29 | 2018-03-13 | 中国水产科学研究院淡水渔业研究中心 | Young shrimp resisting stress dousing agent of a kind of Macrobrachium rosenbergii and preparation method thereof |
| CN108850704A (en) * | 2018-06-26 | 2018-11-23 | 广东海因特生物技术集团有限公司 | Chinese herbal feed additive and its preparation method and application for Penaeus Vannmei |
| CN109105634A (en) * | 2018-07-25 | 2019-01-01 | 东阿阿胶股份有限公司 | A kind of additive for preventing to alleviate donkey transport stress |
| CN110279045A (en) * | 2019-08-01 | 2019-09-27 | 清远海贝生物技术有限公司 | A kind of premix and its preparation method and application preventing golden silvery pomfret irritability gill damage |
| CN110250325A (en) * | 2019-08-02 | 2019-09-20 | 广东海因特生物技术集团有限公司 | A kind of premix and its preparation method and application preventing jewfish irritability enteritis |
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