CN104738098A - Wettable powder for preventing and treating fusarium wilt of cucumber - Google Patents
Wettable powder for preventing and treating fusarium wilt of cucumber Download PDFInfo
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- CN104738098A CN104738098A CN201510131222.1A CN201510131222A CN104738098A CN 104738098 A CN104738098 A CN 104738098A CN 201510131222 A CN201510131222 A CN 201510131222A CN 104738098 A CN104738098 A CN 104738098A
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- fusarium axysporum
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Abstract
The invention discloses wettable powder for preventing and treating fusarium wilt of cucumber. The wettable powder is prepared by the following formula components in parts by weight: 5-10 parts of a wetter, 3-10 parts of a dispersant, 25-35 parts of kieselguhr, 15-35 parts of water, and 5-15 parts of trichoderma harziamum fermentation liquor freeze-dried powder. The wettable powder disclosed by the invention overcomes the problems of environmental toxic and side effects and pesticide residue brought by chemical agents, has the characteristics of being efficient, non-toxic, safe and free of residual, and is an environment-friendly solid microbial inoculum for preventing and treating fusarium wilt of cucumber.
Description
Technical field
The invention belongs to biological pesticide technical field, be specifically related to a kind of wetting powder preventing and treating cucumber fusarium axysporum.
Background technology
Cucumber fusarium axysporum, having another name called wilt disease, dead arm, dead seedling disease, is a kind of by soil infection, invade from root or collar portion, in the entozoic systemic disease of vascular bundle (conduit type fusarium wilt), be one of disease of more difficult control on cucumber production, often cause greater loss.The pathogen of cucumber fusarium axysporum is Deuteromycotina fusarium fungus.This germ is in soil, the residual body of diseased plant and the farmyard manure that do not become thoroughly decomposed or be attached on seed and survive the winter, and becomes primary source of infection in next year, can carry out long-distance communications by rainwater, irrigation water etc.In recent years, fusarium wilt one of Major Diseases rising to vegetables caused by pinch outs (F.oxysprorum Schlecht.), be injured with cucumber in vegetables the most serious, the incidence of disease is generally 20% ~ 30%, and the heavy regional incidence of disease of being injured is up to more than 80% ~ 90%.
Biological control vegetable disease is utilized to not only avoid the problem of a series of environmental pollutions that chemical pesticide brings, and safely, effectively and lastingly, and have not easily make pathogen produce drug resistance, nontoxic to person poultry safety, free from environmental pollution, noresidue, can keep ecological balance, the better simply feature of production technology.But current biological control pharmacy variety is less, effect is fast not as chemical pesticide, and effect is unstable, receives weather influence comparatively large, brings harm to biological control cucumber fusarium axysporum.
Summary of the invention
The technical problem to be solved in the present invention is, provides a kind of wetting powder of control cucumber fusarium axysporum of easy to use, good drug efficacy.
For solving the problems of the technologies described above, the present invention adopts following technical scheme: a kind of wetting powder preventing and treating cucumber fusarium axysporum, this wetting powder is prepared from by the formula of following weight portion: wetting agent 5 ~ 10 parts, dispersant 3 ~ 10 parts, 25 ~ 35 parts, diatomite, 15 ~ 35 parts, water, long handle wood mould zymotic fluid freeze-dried powder 5 ~ 15 parts.
Further, this wetting powder is prepared from by the formula of following weight portion: wetting agent 5 parts, dispersant 3 parts, 25 parts, diatomite, 15 parts, water, long handle wood mould zymotic fluid freeze-dried powder 5 parts.
Further, this wetting powder is prepared from by the formula of following weight portion: wetting agent 10 parts, dispersant 10 parts, 35 parts, diatomite, 35 parts, water, long handle wood mould zymotic fluid freeze-dried powder 15 parts.
Further, described dispersant is CMC.
Further, described wetting agent is glycerine or ethanol.
Further, the described mould zymotic fluid zymotic fluid freeze-dried powder of long handle wood is the fermentation liquor freeze drying of the mould ATCC 52325 of long handle wood obtained.
Further, the preparation method of the zymotic fluid of the described mould ATCC 52325 of long handle wood is as follows:
The mould ATCC 52325 of long handle wood is inoculated in PDA culture fluid, cultivates 2 ~ 5 days, centrifugal segregation thalline, obtain the mould zymotic fluid of long handle wood.
Further, described fermentation, in its fermentation process, temperature is 28 ~ 35 DEG C.
Further, described fermentation, its fermentation process medium speed is 180 ~ 260rpm.Beneficial effect:
The present invention prepares wetting powder with mould ATCC 52325 zymotic fluid of long handle wood, not only overcome environment toxic and side effect and Pesticide Residue that chemical agent brings, and safely, there is no residue of pesticide, be a kind of wetting powder of environmentally friendly green control cucumber fusarium axysporum, have broad application prospects in biological control cucumber fusarium axysporum field.Meanwhile, the present invention also can obtain the antibacterial disease resisting effect same with chemical agent.
Embodiment
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, the content described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
Embodiment 1:
Be inoculated in PDA culture fluid by the mould ATCC52325 of long handle wood, cultivate 2 ~ 5 days, in incubation, temperature controls is 30 DEG C, and rotating speed is 180rpm, obtains the mould zymotic fluid of long handle wood.By zymotic fluid centrifugal segregation thalline, by supernatant freeze drying.
Embodiment 2:
Following material is mixed according to weight portion: glycerine 5 parts, CMC 3 parts, 25 parts, diatomite, 15 parts, water, long handle wood mould zymotic fluid freeze-dried powder 5 parts.Mix and pulverize, through the screen filtration of 30 μm, the wetting powder of cucumber fusarium axysporum must be prevented and treated.
Embodiment 3:
Following material is mixed according to weight portion: ethanol 10 parts, CMC 10 parts, 35 parts, diatomite, 35 parts, water, long handle wood mould zymotic fluid freeze-dried powder 15 parts.Mix and pulverize, through the screen filtration of 30 μm, the wetting powder of cucumber fusarium axysporum must be prevented and treated.
Embodiment 4:
Experimental period: 2014
Controlling object: cucumber fusarium axysporum
Experiment place: Jiu Jiu village, Fu An town, Dongtai City of Jiangsu Province, soil erosion 4 mu, soil organic matter content 2.5%, yellow spacing in the rows 50cm, line-spacing 2m.
Experimental pharmacy: the wetting powder of control control cucumber fusarium axysporum provided by the invention, dilutes 200 times, 400 times, 600 times, 800 times, 1000 times respectively.
Contrast agents: tpn wetting powder, dilutes 200 times, 400 times, 600 times, 800 times, 1000 times respectively.
Application method: cucumber Post flowering sprays once, after this sprays once week about.
Drug effect computational methods application reference number is the computational methods in the Chinese patent of 201410303095.4, and control efficiency is in table 1.
The control efficiency of wetting powder to cucumber fusarium axysporum of cucumber fusarium axysporum prevented and treated by table 1
| Chemicals treatment | Disease index | Control efficiency |
| Wetting powder-200 times | 1.8 | 87％ |
| Wetting powder-400 times | 1.3 | 82％ |
| Wetting powder-600 times | 1.5 | 83％ |
| Wetting powder-800 times | 2.1 | 85％ |
| Wetting powder-1000 times | 2.3 | 78％ |
| Tpn-200 times | 1.3 | 81％ |
| Tpn-400 times | 2.1 | 77％ |
| Tpn-600 times | 1.8 | 82％ |
| Tpn-800 times | 1.5 | 80％ |
| Tpn-1000 times | 1.2 | 75％ |
| Contrast-water | 4.0 | - |
As can be seen from Table 1, the wetting powder of control cucumber fusarium axysporum effectively can prevent and treat cucumber fusarium axysporum, is up to 87% to the inhibiting rate of cucumber fusarium axysporum.
Claims (9)
1. prevent and treat the wetting powder of cucumber fusarium axysporum for one kind, it is characterized in that, this wetting powder is prepared from by the formula of following weight portion: wetting agent 5 ~ 10 parts, dispersant 3 ~ 10 parts, 25 ~ 35 parts, diatomite, 15 ~ 35 parts, water, long handle wood mould zymotic fluid freeze-dried powder 5 ~ 15 parts.
2. the wetting powder of control cucumber fusarium axysporum according to claim 1, it is characterized in that, this wetting powder is prepared from by the formula of following weight portion: wetting agent 5 parts, dispersant 3 parts, 25 parts, diatomite, 15 parts, water, long handle wood mould zymotic fluid freeze-dried powder 5 parts.
3. the wetting powder of control cucumber fusarium axysporum according to claim 1, it is characterized in that, this wetting powder is prepared from by the formula of following weight portion: wetting agent 10 parts, dispersant 10 parts, 35 parts, diatomite, 35 parts, water, long handle wood mould zymotic fluid freeze-dried powder 15 parts.
4., according to the wetting powder of the arbitrary described control cucumber fusarium axysporum of claims 1 to 3, it is characterized in that, described dispersant is CMC.
5., according to the wetting powder of the arbitrary described control cucumber fusarium axysporum of claims 1 to 3, it is characterized in that, described wetting agent is glycerine or ethanol.
6. the wetting powder of control cucumber fusarium axysporum according to claim 1, is characterized in that, the described mould zymotic fluid zymotic fluid freeze-dried powder of long handle wood is the fermentation liquor freeze drying of the mould ATCC 52325 of long handle wood obtained.
7. the wetting powder of control cucumber fusarium axysporum according to claim 1, is characterized in that, the preparation method of the zymotic fluid of the described mould ATCC 52325 of long handle wood is as follows:
The mould ATCC 52325 of long handle wood is inoculated in PDA culture fluid, cultivates 2 ~ 5 days, centrifugal segregation thalline, obtain the mould zymotic fluid of long handle wood.
8. the wetting powder of control cucumber fusarium axysporum according to claim 7, is characterized in that, described fermentation, and in its fermentation process, temperature is 28 ~ 35 DEG C.
9. the wetting powder of control cucumber fusarium axysporum according to claim 7, is characterized in that, described fermentation, and its fermentation process medium speed is 180 ~ 260rpm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510131222.1A CN104738098A (en) | 2015-03-24 | 2015-03-24 | Wettable powder for preventing and treating fusarium wilt of cucumber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510131222.1A CN104738098A (en) | 2015-03-24 | 2015-03-24 | Wettable powder for preventing and treating fusarium wilt of cucumber |
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| Publication Number | Publication Date |
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| CN104738098A true CN104738098A (en) | 2015-07-01 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510131222.1A Pending CN104738098A (en) | 2015-03-24 | 2015-03-24 | Wettable powder for preventing and treating fusarium wilt of cucumber |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105779302A (en) * | 2016-03-24 | 2016-07-20 | 江西天人生态股份有限公司 | Hypocrea virens-containing composite microbial agent for controlling cucumber fusarium wilt, and preparation method of hypocrea virens-containing composite microbial agent |
| CN107410373A (en) * | 2017-06-08 | 2017-12-01 | 合肥龙滨化工科技有限公司 | A kind of medicine for preventing and treating cucumber fusarium axysporum and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102212485A (en) * | 2011-05-09 | 2011-10-12 | 中国农业科学院农业资源与农业区划研究所 | Trichoderma longibrachiatum and application thereof to preventing and treating vegetable diseases |
-
2015
- 2015-03-24 CN CN201510131222.1A patent/CN104738098A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102212485A (en) * | 2011-05-09 | 2011-10-12 | 中国农业科学院农业资源与农业区划研究所 | Trichoderma longibrachiatum and application thereof to preventing and treating vegetable diseases |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105779302A (en) * | 2016-03-24 | 2016-07-20 | 江西天人生态股份有限公司 | Hypocrea virens-containing composite microbial agent for controlling cucumber fusarium wilt, and preparation method of hypocrea virens-containing composite microbial agent |
| CN107410373A (en) * | 2017-06-08 | 2017-12-01 | 合肥龙滨化工科技有限公司 | A kind of medicine for preventing and treating cucumber fusarium axysporum and preparation method thereof |
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Application publication date: 20150701 |
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