CN104737818A - Method for purifying edible fungi test tube species - Google Patents
Method for purifying edible fungi test tube species Download PDFInfo
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- CN104737818A CN104737818A CN201510164532.3A CN201510164532A CN104737818A CN 104737818 A CN104737818 A CN 104737818A CN 201510164532 A CN201510164532 A CN 201510164532A CN 104737818 A CN104737818 A CN 104737818A
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- test tube
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- edible fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The invention discloses a method for purifying edible fungi test tube species. The method specifically comprises the following steps of: preparing PDA slant tubes by a routine method, sterilizing and disinfecting to the slant tubes, cooling about 45 degrees centigrade, respectively injecting 300 times of an isothiazolinone solution into 10 slant tube culture mediums by a sterile syringe, shaking the culture mediums to be fully mixed, then and immediately configuring slopes to prepare anti-fungal test tubes; under sterile environment, moving strained polluted by germs to inclined culture mediums of the anti-fungal test tubes by a sterile operation method, then placing at 25 degrees centigrade and culturing until fully filling the tubes with hyphae, finally transferring the strains fully filled on the tubes into the anti-fungal test tubes according to the method, and removing germs through twice transfer, thereby preparing excellent test-tube maternal strains. The method for purifying polluted edible fungi mother spawns has unique measures, thus the method is easy to operate. Meanwhile the method has the good infected bacteria removal effect, and but edible fungi mycelium can keep high quality by removing the infected mother strains instead of being inhibited.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of purification process of edible mushroom test tube kind.
Technical background
The quality of parent edible fungus kind is the key of edible fungus high yield.In Edible Fungi process, before edible mushroom, two-stage bacterial classification must be cultivated by purifying, otherwise will affect output, even can suffer larger loss.
At present, Edible Fungi person, once find that there is the bacterial classification of pollution, is just abandoned immediately.Some rare edible fungus species is not only rare, and very famous and precious, abandons like this, loses very large.
Summary of the invention
Object of the present invention is exactly the purification process overcoming the deficiencies in the prior art and provide brand-new a kind of edible mushroom test tube kind.Specifically comprise: prepare PDA slant tube according to a conventional method, then by test tube through sterilization, when test tube is cooled to about 45 DEG C, the KF88s of 300 times (isothiazolinone) solution asepsis injector is injected 10 slant tube medium respectively, and shake medium and make it abundant mixing, then put immediately inclined-plane and antibacterial test tube; Then in an aseptic environment, the bacterial classification be contaminated by bacterial is moved in the slant medium of antibacterial test tube with sterile working method, then be cultured to mycelia at being positioned over 25 DEG C and cover with pipe, finally the bacterial classification covering with pipe is transferred again in antibacterial test tube more as stated above, bacterium can be got rid of through twice tube, then obtain the test tube stock of high-quality.The purification process measure of pollution parent edible fungus kind proposed by the invention is unique, and when implementing, technique is simple.Meanwhile, by implementing the present invention, effect being got rid of to the bacterium infected excellent, and to hypha of edible fungus unrestraint effect, planting maintenance high-quality through the mother getting rid of bacteriological infection.
Described KF88 is the transliteration of English KATONH, and active ingredient is the mixture of isothiazolinone CIT and MIT.CAS:26172-55-4,2682-20-4; Molecular formula: C4H4C1NOS+C4H5NOS; Molecular weight: 149.56+115.06.KF88 broad-spectrum is efficient, nontoxic, lasting effect is long, can effectively suppress and go out except mushroom and various microorganism; KF88 safe ready, stable in properties, addition is few, all applicable in the scope of pH value 3-9.5; KF88 environmental protection, environmentally friendly, not containing harmful substances such as formaldehyde heavy metals.
The present invention is achieved through the following technical solutions:
A purification process for edible mushroom test tube kind, is in technical scheme to comprise the steps:
1, antibacterial test tube is prepared: prepare PDA slant tube according to a conventional method, then by test tube through sterilization, when test tube is cooled to about 45 DEG C, the KF88s of 300 times (isothiazolinone) solution asepsis injector is injected 10 slant tube medium respectively, and shake medium and make it abundant mixing, then put immediately inclined-plane and antibacterial test tube.
2, biography connects infection bacteria species: in an aseptic environment, the bacterial classification be contaminated by bacterial is moved in the slant medium of antibacterial test tube with sterile working method, then be cultured to mycelia at being positioned over 25 DEG C and cover with pipe, then the bacterial classification covering with pipe is transferred again in antibacterial test tube more as stated above, bacterium can be got rid of through twice tube, then obtain the test tube stock of high-quality.
The present invention both had the following advantages:
The purification process measure of pollution parent edible fungus kind 1, proposed by the invention is unique, and when implementing, technique is simple.
2, by implementing the present invention, effect being got rid of to the bacterium infected excellent, and to hypha of edible fungus unrestraint effect, planting maintenance high-quality through the mother getting rid of bacteriological infection.
Embodiment
Below in conjunction with embodiment, method of the present invention is further illustrated.
A purification process for edible mushroom test tube kind, embodiment is as follows:
1, antibacterial test tube is prepared: prepare PDA slant tube according to a conventional method, then by test tube through sterilization, when test tube is cooled to about 45 DEG C, the KF88s of 300 times (isothiazolinone) solution asepsis injector is injected 10 slant tube medium respectively, and shake medium and make it abundant mixing, then put immediately inclined-plane and antibacterial test tube.
2, biography connects infection bacteria species: in an aseptic environment, the bacterial classification be contaminated by bacterial is moved in the slant medium of antibacterial test tube with sterile working method, then be cultured to mycelia at being positioned over 25 DEG C and cover with pipe, then the bacterial classification covering with pipe is transferred again in antibacterial test tube more as stated above, bacterium can be got rid of through twice tube, then obtain the test tube stock of high-quality.
Claims (1)
1. a purification process for edible mushroom test tube kind, is characterized in that comprising the steps:
(1) antibacterial test tube is prepared: prepare PDA slant tube according to a conventional method, then by test tube through sterilization, when test tube is cooled to about 45 DEG C, the KF88s of 300 times (isothiazolinone) solution asepsis injector is injected 10 slant tube medium respectively, and shake medium and make it abundant mixing, then put immediately inclined-plane and antibacterial test tube;
(2) biography connects infection bacteria species: in an aseptic environment, the bacterial classification be contaminated by bacterial is moved in the slant medium of antibacterial test tube with sterile working method, then be cultured to mycelia at being positioned over 25 DEG C and cover with pipe, then the bacterial classification covering with pipe is transferred again in antibacterial test tube more as stated above, bacterium can be got rid of through twice tube, then obtain the test tube stock of high-quality.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510164532.3A CN104737818A (en) | 2015-04-09 | 2015-04-09 | Method for purifying edible fungi test tube species |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510164532.3A CN104737818A (en) | 2015-04-09 | 2015-04-09 | Method for purifying edible fungi test tube species |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104737818A true CN104737818A (en) | 2015-07-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201510164532.3A Pending CN104737818A (en) | 2015-04-09 | 2015-04-09 | Method for purifying edible fungi test tube species |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107637390A (en) * | 2017-11-03 | 2018-01-30 | 江苏农林职业技术学院 | A kind of live body silkworm and the method for the wild cicada fungus of silkworm chrysalis culture |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102308750A (en) * | 2011-07-26 | 2012-01-11 | 天津滨城龙达集团有限公司 | Plant tissue culture bacteriostatic (antiseptic) agent containing botanical fungicide |
| CN102763662A (en) * | 2012-05-31 | 2012-11-07 | 中国农业大学 | Medium for separating Peronophythora Litchi Chen ex Ko et al |
| CN103155861A (en) * | 2011-12-10 | 2013-06-19 | 何寒 | Plant open-type issue culture seedling method |
| CN103329800A (en) * | 2013-06-17 | 2013-10-02 | 何寒 | Tissue culture medium without agar during plant open tissue culturing and preparation method of tissue culture medium |
-
2015
- 2015-04-09 CN CN201510164532.3A patent/CN104737818A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102308750A (en) * | 2011-07-26 | 2012-01-11 | 天津滨城龙达集团有限公司 | Plant tissue culture bacteriostatic (antiseptic) agent containing botanical fungicide |
| CN103155861A (en) * | 2011-12-10 | 2013-06-19 | 何寒 | Plant open-type issue culture seedling method |
| CN102763662A (en) * | 2012-05-31 | 2012-11-07 | 中国农业大学 | Medium for separating Peronophythora Litchi Chen ex Ko et al |
| CN103329800A (en) * | 2013-06-17 | 2013-10-02 | 何寒 | Tissue culture medium without agar during plant open tissue culturing and preparation method of tissue culture medium |
Non-Patent Citations (2)
| Title |
|---|
| 丁湖广等: "《食用菌菌种规范化生产技术问答》", 31 December 2010 * |
| 陈士瑜: "《食用菌斜面菌种的纯化》", 《食用菌》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107637390A (en) * | 2017-11-03 | 2018-01-30 | 江苏农林职业技术学院 | A kind of live body silkworm and the method for the wild cicada fungus of silkworm chrysalis culture |
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Application publication date: 20150701 |
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