CN102726290A - Method for eliminating tissue culture seedling pollution - Google Patents
Method for eliminating tissue culture seedling pollution Download PDFInfo
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- CN102726290A CN102726290A CN2011100906120A CN201110090612A CN102726290A CN 102726290 A CN102726290 A CN 102726290A CN 2011100906120 A CN2011100906120 A CN 2011100906120A CN 201110090612 A CN201110090612 A CN 201110090612A CN 102726290 A CN102726290 A CN 102726290A
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Abstract
The invention relates to a method for eliminating tissue culture seedling pollution, and the method is characterized by including the following steps of: (1) adding a sodium hypochlorite solution into a culture medium used for culturing tissue culture seedlings; (2) transferring a polluted tissue culture seedling to the sodium hypochlorite solution-containing culture medium; (3) repeating step (1) and (2), and conducting transfer several times so as to eliminate pollution; and (4) culturing the pollution eliminated tissue culture seedling. The method of the invention has the advantages that: compared with other antibiotics, the sodium hypochlorite solution adopted in the invention is convenient to add and can be subjected to autoclaving along with the culture medium; compared with mercuric chloride, the sodium hypochlorite solution has no secondary pollution, and tissue culture seedling pollution can be effectively eliminated; and the method can be used to save polluted tissue culture seedlings in tissue culture seedling species preservation and rapid propagation of seedlings for production, thus boasting substantial ecological and economic benefits.
Description
Technical field
The present invention relates to a kind of method of eliminating the tissue culture seedling pollution, concrete is a kind of method of eliminating the tissue culture seedling pollution with clorox.
Background technology
In the plant tissue culture process; Contamination phenomenon is to organize one of Three Difficult Issues in the training process at present; Because a variety of causes, cause tissue cultivating seedling germ contamination to occur, the tissue culturing seedling after contaminated generally is doing in addition of abandoning; Cause the raising of production cost and the delay in farming season, the preservation of plant resource is often caused the loss that can't retrieve.In the group training at present; Adopt antibiotic to carry out sterilization processing, but do not find that so far the sort of antibiotic can be all effective to various bacterium, effect is undesirable; The employing mercury chloride that also has is prevented and treated; But mercury chloride causes second environmental pollution easily after using, more preferred with its more this method.
Summary of the invention
The purpose of this invention is to provide a kind of method of eliminating the tissue culture seedling pollution, the sterilization effect that prior art exists is undesirable to solve, complicated operation, cause secondary pollution and do not possess the problem of promotional value easily.Technical scheme of the present invention is: a kind of method of eliminating the tissue culture seedling pollution, it is characterized in that, and may further comprise the steps:
(1) cultured tissue is cultivated in the medium of seedling adds the liquor natrii hypochloritis;
(2) tissue culturing seedling that pollutes is switched on the medium that contains the liquor natrii hypochloritis;
(3) repeating step (1) and (2) after switching through for several times, get final product pollution abatement;
(4) tissue culturing seedling of pollution abatement is cultivated get final product.
The described cycle of switching through at every turn is 5-25 days.
Described liquor natrii hypochloritis's concentration is 5-50mg/L.
The pH value of described medium is 5.5-5.8, shows slightly acid.
The cultivation temperature 22-24 of above-mentioned tissue culturing seedling ℃, illumination condition 10-12 every day hour is about intensity of illumination 2000lx.
Clorox concentration is higher in the described medium, and bactericidal effect is good, so concentration is big more; The cycle of switching through is short more, avoids the injury to tissue cultivating seedling as far as possible, according to the content of clorox in the medium; The cycle is switched through in arrangement, and general clorox concentration is switched through the cycle from long to short from low to high; According to the actual growing state of tissue culturing seedling, adjustment is switched through the cycle.
Advantage of the present invention is: compare with other antibiotic, it is convenient to add, can be with the medium autoclaving; Compare with mercury chloride; Do not have secondary pollution, can effectively eliminate the pollution of tissue cultivating seedling, in the tissue cultivating seedling species are preserved and produce with Miao Kuaifan; Save and pollute tissue cultivating seedling, have very big ecological benefits and economic benefit.
Embodiment
Embodiment 1: the elimination of the big and taro tissue culture seedling pollution of Japan
Japan's tissue cultivating seedling big and taro obtains through growth top differentiation culture, switches through in the process, because misoperation or endophyte cause tissue culture seedling pollution, bacterial plaque occurs at root or media surface.Tissue cultivating seedling with polluting contains in the medium that concentration is the 5mg/L clorox through switching through technology normally, being switched to; Making the pH value of its medium is 5.5, makes the condition of culture of tissue culturing seedling: 22 ℃ of temperature, 12 hours every days of illumination condition; Intensity of illumination 2000lx, per 10 days is one-period, through behind the one-period; According to bacterial plaque and tissue cultivating seedling upgrowth situation, again tissue culturing seedling is switched on the medium that contains new liquor natrii hypochloritis, 3 all after dates so circulate; Pollution abatement obtains healthy and strong tissue cultivating seedling.
Embodiment 2: the elimination of hemerocailis middendorffi tissue culture seedling pollution
The hemerocailis middendorffi tissue cultivating seedling that pollutes is contained in the medium that concentration is the 20mg/L clorox through switching through technology normally, being switched to, and the pH value that makes its medium is 5.7; Make the condition of culture of tissue culturing seedling: 24 ℃ of temperature, 12 hours every days of illumination condition, intensity of illumination 2000lx; Per 20 days is one-period, through behind the one-period, according to bacterial plaque and tissue cultivating seedling upgrowth situation; Again tissue culturing seedling is switched on the medium that contains new liquor natrii hypochloritis; 3 all after dates of circulation like this, pollution abatement obtains healthy and strong tissue cultivating seedling.
Examples of implementation 3: the elimination of cut-flower chrysanthemum tissue culture seedling pollution
The cut-flower chrysanthemum tissue cultivating seedling that pollutes is contained in the medium that concentration is the 50mg/L clorox through switching through technology normally, being switched to, and the pH value that makes its medium is 5.8; Condition of culture: 24 ℃ of temperature, 12 hours every days of illumination condition, intensity of illumination 2000lx; Per 10 days is one-period; Through 2 all after dates, get final product pollution abatement, obtain healthy and strong tissue cultivating seedling.
Claims (5)
1. a method of eliminating the tissue culture seedling pollution is characterized in that, may further comprise the steps:
(1) cultured tissue is cultivated in the medium of seedling add the liquor natrii hypochloritis, the concentration of clorox is 5-50mg/L in the medium
(2) tissue culturing seedling that pollutes is switched on the medium that contains the liquor natrii hypochloritis;
(3) repeating step (1) and (2) after switching through for several times, get final product pollution abatement;
(4) tissue culturing seedling of pollution abatement is cultivated get final product.
2. the method for elimination tissue culture seedling pollution according to claim 1 is characterized in that, the described cycle of switching through at every turn is 5-25 days.
3. the method for elimination tissue culture seedling pollution according to claim 1 is characterized in that the pH value of described medium is 5.5-5.8, shows slightly acid.
4. the method for elimination tissue culture seedling pollution according to claim 1 is characterized in that, cultivation temperature 22-24 ℃ of the tissue culturing seedling in the described step (4), and illumination condition 10-12 every day hour is about intensity of illumination 2000lx.
5. according to the method for any described elimination tissue culture of the claim seedling pollution of claim 1 to 4, it is characterized in that clorox concentration is higher in the described medium; Bactericidal effect is good, so concentration is big more, the cycle of switching through is short more; According to the content of clorox in the medium, to arrange to switch through the cycle, general clorox concentration is from low to high; Switch through the cycle from long to short, according to the actual growing state of tissue culturing seedling, adjustment is switched through the cycle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100906120A CN102726290A (en) | 2011-04-12 | 2011-04-12 | Method for eliminating tissue culture seedling pollution |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011100906120A CN102726290A (en) | 2011-04-12 | 2011-04-12 | Method for eliminating tissue culture seedling pollution |
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| CN102726290A true CN102726290A (en) | 2012-10-17 |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105494106A (en) * | 2016-02-05 | 2016-04-20 | 王少荣 | Method for eliminating mold contamination to blueberry tissue culture seedlings |
| CN105519444A (en) * | 2016-02-05 | 2016-04-27 | 王少荣 | Method for saving bacterial pollution of chrysanthemum morifolium tissue culture seedlings |
| CN105519443A (en) * | 2016-02-05 | 2016-04-27 | 王少荣 | Method for saving mould pollution of Chrysanthemum shiwogiku var. kinokuniense tissue culture seedlings |
| CN105532478A (en) * | 2016-02-05 | 2016-05-04 | 王少荣 | Method for saving bacterial contamination of tissue culture seedlings of chrysanthemum yoshinaganthum |
| CN105557516A (en) * | 2015-12-14 | 2016-05-11 | 广西壮族自治区药用植物园 | Method for eliminating bacterial pollution of tissue culture seedlings |
| CN105613299A (en) * | 2016-02-05 | 2016-06-01 | 王少荣 | Method for eliminating bacterial contamination of chrysanthemum tissue cultured seedlings |
| CN105660403A (en) * | 2016-02-05 | 2016-06-15 | 王少荣 | Method for saving Chuzhou chrysanthemum tissue culture seedling from mold contamination |
| CN105684906A (en) * | 2016-02-05 | 2016-06-22 | 王少荣 | Method for remedying mold contamination of tissue culture seedlings of Nakagawa-cho chrysanthemum indicum |
| CN105684907A (en) * | 2016-02-05 | 2016-06-22 | 王少荣 | Method for remedying bacterial contamination of tissue culture seedlings of Stevia rebaudiana |
| CN105684909A (en) * | 2016-02-05 | 2016-06-22 | 王少荣 | Method for remedying mold contamination of tissue culture seedlings of oceanic chrysanthemum |
| CN106035092A (en) * | 2016-07-18 | 2016-10-26 | 中国科学院新疆生态与地理研究所 | Method for recovering and rejuvenating moss in culture medium polluted by fungus and mold |
| CN106332784A (en) * | 2016-11-10 | 2017-01-18 | 福建农林大学 | Open gerbera jamesonii tissue culture method |
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| JPH07255305A (en) * | 1994-03-28 | 1995-10-09 | Kagome Co Ltd | Method for culturing plant tissue or seedling material |
| CN101627727A (en) * | 2009-08-25 | 2010-01-20 | 山东省烟台农业学校 | Method for preventing and controlling tissue culture seedling pollution |
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2011
- 2011-04-12 CN CN2011100906120A patent/CN102726290A/en active Pending
Patent Citations (2)
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|---|---|---|---|---|
| JPH07255305A (en) * | 1994-03-28 | 1995-10-09 | Kagome Co Ltd | Method for culturing plant tissue or seedling material |
| CN101627727A (en) * | 2009-08-25 | 2010-01-20 | 山东省烟台农业学校 | Method for preventing and controlling tissue culture seedling pollution |
Non-Patent Citations (1)
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105557516A (en) * | 2015-12-14 | 2016-05-11 | 广西壮族自治区药用植物园 | Method for eliminating bacterial pollution of tissue culture seedlings |
| CN105494106A (en) * | 2016-02-05 | 2016-04-20 | 王少荣 | Method for eliminating mold contamination to blueberry tissue culture seedlings |
| CN105519444A (en) * | 2016-02-05 | 2016-04-27 | 王少荣 | Method for saving bacterial pollution of chrysanthemum morifolium tissue culture seedlings |
| CN105519443A (en) * | 2016-02-05 | 2016-04-27 | 王少荣 | Method for saving mould pollution of Chrysanthemum shiwogiku var. kinokuniense tissue culture seedlings |
| CN105532478A (en) * | 2016-02-05 | 2016-05-04 | 王少荣 | Method for saving bacterial contamination of tissue culture seedlings of chrysanthemum yoshinaganthum |
| CN105613299A (en) * | 2016-02-05 | 2016-06-01 | 王少荣 | Method for eliminating bacterial contamination of chrysanthemum tissue cultured seedlings |
| CN105660403A (en) * | 2016-02-05 | 2016-06-15 | 王少荣 | Method for saving Chuzhou chrysanthemum tissue culture seedling from mold contamination |
| CN105684906A (en) * | 2016-02-05 | 2016-06-22 | 王少荣 | Method for remedying mold contamination of tissue culture seedlings of Nakagawa-cho chrysanthemum indicum |
| CN105684907A (en) * | 2016-02-05 | 2016-06-22 | 王少荣 | Method for remedying bacterial contamination of tissue culture seedlings of Stevia rebaudiana |
| CN105684909A (en) * | 2016-02-05 | 2016-06-22 | 王少荣 | Method for remedying mold contamination of tissue culture seedlings of oceanic chrysanthemum |
| CN106035092A (en) * | 2016-07-18 | 2016-10-26 | 中国科学院新疆生态与地理研究所 | Method for recovering and rejuvenating moss in culture medium polluted by fungus and mold |
| CN106332784A (en) * | 2016-11-10 | 2017-01-18 | 福建农林大学 | Open gerbera jamesonii tissue culture method |
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Application publication date: 20121017 |