CN104713750A - End gas sampling apparatus for detecting volatile organic compounds in expired air - Google Patents
End gas sampling apparatus for detecting volatile organic compounds in expired air Download PDFInfo
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- CN104713750A CN104713750A CN201310691210.5A CN201310691210A CN104713750A CN 104713750 A CN104713750 A CN 104713750A CN 201310691210 A CN201310691210 A CN 201310691210A CN 104713750 A CN104713750 A CN 104713750A
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Abstract
The invention discloses an end air sampling apparatus for detecting volatile organic compounds in expired air. The main body of the apparatus is a sample bottle with the volume of 20-100mL. Two ends of the sampling bottle are provided with a gas inlet and a gas outlet respectively, and are respectively connected with a valve; and the middle of the sampling bottle is provided with a small hole as a mass spectrum sample introduction port. When the apparatus is used to sample, the valves at two ends of the apparatus are opened, a tester blows air to a detachable air blow tube at one end of the sampling bottle, the valves at two ends are immediately closed after the breath is completed, that is, initial and intermediate stage air flows out, and end air is corresponding collected by the 20-100mL sampling bottle. The apparatus avoids interference of initial and intermediate air to measurement, is in favor of detecting end air, integrates sampling and sample introduction, and allows a collected sample to be carried by a carrier gas and to be analyzed through mass spectrometry in real time, so the apparatus is very suitable for detecting the volatile organic compounds in end expired air.
Description
Technical field
The invention belongs to the collection of end gas in expiratory air, particularly a kind of mass spectrum chemi-ionization mode that adopts carries out the sampling apparatus measured.
Background technology
The portion of product of human metabolism can be transported to lung by blood, enters alveolar and appear in breath by gas exchanges.A kind of method that breath detects as the human physiological metabolism's of understanding process and monitoring human disease condition has great importance, and is subject to people and more and more payes attention to.Compared with the blood testing of routine, the great advantage that expiratory air detects is to human zero damage, and operating process is convenient and swift.
The reliable method of sampling analyzes successful important guarantee.The concentration of characteristics of contaminated respiratory droplets gas is generally in ppb rank, and content is very low.Therefore, the key of expiratory air collection is to make sample do not diluted as far as possible, is not subject to the interference of gas in environment.Gas concentration in human body alveolar is directly related with own metabolism process, should collect alveolar gas as far as possible during sampling.In the process of human body breath each time, starting the gas of breathing out can be diluted by the dead volume between from lung to sampling apparatus, causes breath concentration to decline; Along with the carrying out of exhalation process, the gas concentration collected moves closer to alveolar concentration and reaches balance.In order to reduce the dilution effect of dead volume, only can collect the latter half volume of expiratory air, i.e. end gas, obtains the gas close to true alveolar concentration with this.This just require by sampling apparatus is carried out reasonably structural design and experimental implementation to collect the expiratory air of human body end.
In expiratory air end gas, the detection method of volatile organic matter comprises chromatography, mass spectroscopy, spectroscopic methodology, sensor technology etc.Wherein mass spectrometry is a kind of important analysis means analyzed by the mensuration of the mass-to-charge ratio (m/z) to sample ion, have high sensitivity, fast, can the advantage such as the accurate molecular quality of sampling simultaneously and structural information.Adopt the volatile organic matter in the expiratory air of mass spectrometry mensuration, can directly obtain its molecular weight information, realize qualitative and quantitative analysis.
Traditional offline inspection has to pass through sample collection, pre-treatment and is separated multiple steps such as detection, and expiratory air end gas composition is very complicated and content is very low, and sample very easily causes in the process of preservation, transport to be lost or go bad.Therefore the real time on-line monitoring development of breath, the detection technique being especially representative with organism on-line monitoring mass-spectrometric technique is subject to people and more and more payes attention to.
Summary of the invention
The object of the present invention is to provide a kind of end gas sampling apparatus detected for volatile organic matter in expiratory air.
A kind of end gas sampling apparatus detected for volatile organic matter in expiratory air:
Comprise gas blow pipe, carrier gas air intake opening, T-valve, device sealed valve A and device sealed valve B, sampling bottle, mass spectrum injection port six part;
Gas blow pipe is the one section of pipeline of blowing for testee being connected to sampling bottle one end; Device sealed valve A, device sealed valve B are connected to the two ends of sampling bottle; T-valve is positioned at one end of device sealed valve A, and two other outlet of its T-valve connects gas blow pipe and carrier gas air intake opening respectively; Mass spectrum injection port is positioned at the upper end of sampling bottle.
Gas blow pipe internal diameter is 2-3mm, length is 5-8cm, to volatile organic matter to be measured in expiratory air without specific adsorption.
Before testee blows, by carrier gas air intake opening, background air in sampling bottle blows away by carrier gas, and the material that may exist in minimizing background air is to the interference measured; When testee blows and samples, by carrier gas air intake opening, the end expiratory air carrier band collected in sampling bottle is entered mass spectrometer and measures by carrier gas.
During sampling, the sealed valve A at device two ends, sealed valve B opens, tester blows facing to gas blow pipe, person to be tested once exhales completely, namely, after the initial and stage casing gas outflow of expiratory air, two ends sealed valve A, sealed valve B are shut immediately, what now collect in sampling bottle is end expiratory air.
The active volume of sampling bottle is 20-100mL, when testee adopts normal exhalation mode, when once exhaling complete, and the end expiratory air of what sampling bottle collected is testee.
Sample gas directly enters the mass spectrum injection port of sampling bottle upper end under carrier gas carrier band, carries out real-time analysis.
Beneficial effect of the present invention is:
One, sampling bottle volume 20-100mL, what can ensure to collect in bottle is end expiratory air.
Two, by the open and close of by-pass valve control, the collection of expiratory air end gas can conveniently be realized.
Three, relatively common sampler bag, in sampling bottle, the few and sampling bottle of impurity itself is to various organism to be measured in expiratory air almost without absorption, and the loss of adsorbing the determinand caused because of harvester drops to minimum.
Four, gas blow pipe internal diameter and suitable length, dead volume is little, to volatile organic matter to be measured in expiratory air without specific adsorption.
Five, mass spectrum injection port is directly inserted in sampling bottle, and the sample collected can by mass spectrum real-time analysis.
Six, package unit centralized procurement sample and sample introduction are in one, simple to operate, convenient and swift.
Accompanying drawing explanation
Fig. 1 is the end gas sampling apparatus that the present invention detects for volatile organic matter in expiratory air.
Wherein, 1-gas blow pipe; 2-carrier gas air intake opening; 3-T-valve; 4-sealed valve A; 5-sealed valve B; 6-sampling bottle; 7-mass spectrum injection port.
Fig. 2 is the analytical data of mass spectrum that the present invention detects for end volatile organic matter in expiratory air.
Embodiment
As shown in Figure 1: 1 is gas blow pipe, i.e. testee's inflatable mouth; 2 is carrier gas air intake opening; 3 is T-valve; 4 is sealed valve A; 5 is sealed valve B; 6 is sampling bottle; 7 is mass spectrum injection port.
Before testee blows, the valve at sampling bottle two ends is all opened, and carrier gas enters from air intake opening 2 with certain flow velocity, and purge after 2-3 minute, in sampling bottle, background air is blown away entirely, adopts Background; After testee deeply breathes, blow facing to clean gas blow pipe 1 immediately, at the end of its sensation is exhaled, the initial and stage casing gas of expiratory air has been blown sampling bottle 6, immediately two ends sealed valve A4, sealed valve B5 are closed, now collect in sampling bottle 6 and be end expiratory air; Carrier gas carries the end gas collected and enters mass spectrometer by mass spectrum injection port 7, and mass spectrographic acquisition software can be utilized to carry out data collection and analysis.
Fig. 2: first put up by determinator, connects carrier gas gas circuit, this experiment with oxygen as carrier gas; Secondly the time of-flight mass spectrometer of pilot scale study is to optimum Working; Finally start experiment: before first step testee blows, all opened by the valve at sampling bottle two ends, oxygen enters from air intake opening 2 with the flow velocity of 100ml/min, purge after 2 minutes, the P7888 data acquisition software carried with instrument adopts Background, continuous acquisition 3 times, record data; Second step testee blows facing to gas blow pipe 1, and at the end of its sensation is exhaled, the initial and stage casing gas of expiratory air is blown sampling bottle, now immediately by two ends valve closing; The P7888 data acquisition software carried with instrument starts collected specimens spectrogram, continuous acquisition 3 times, record data.All data importing Origin software is carried out data processing: get average background value and sample mean, map after background correction, obtain Fig. 2.From Fig. 2, can see, successfully record acetone, isoprene, methyl alcohol, the ethanol of ppb rank in end gas by this method.
Claims (6)
1., for the end gas sampling apparatus that volatile organic matter in expiratory air detects, it is characterized in that:
Comprise gas blow pipe (1), carrier gas air intake opening (2), T-valve (3), device sealed valve A(4) and device sealed valve B(5), sampling bottle (6), mass spectrum injection port (7) six part;
Gas blow pipe (1) is the one section of pipeline of blowing for testee being connected to sampling bottle (6) one end; Device sealed valve A(4), device sealed valve B(5) be connected to the two ends of sampling bottle (6); T-valve (3) is positioned at device sealed valve A(4) one end, two other outlet of its T-valve (3) connects gas blow pipe (1) and carrier gas air intake opening (2) respectively; Mass spectrum injection port (7) is positioned at the upper end of sampling bottle (6).
2. the end gas sampling apparatus detected for volatile organic matter in expiratory air according to claim 1, is characterized in that:
Gas blow pipe (1) internal diameter is 2-3mm, length is 5-8cm, to volatile organic matter to be measured in expiratory air without specific adsorption.
3. the end gas sampling apparatus detected for volatile organic matter in expiratory air according to claim 1, is characterized in that:
Before testee blows, by carrier gas air intake opening (2), sampling bottle (6) interior background air blows away by carrier gas, and the material that may exist in minimizing background air is to the interference measured; When testee blows and samples, by carrier gas air intake opening (2), the end expiratory air carrier band collected in sampling bottle (6) is entered mass spectrometer and measures by carrier gas.
4. the end gas sampling apparatus detected for volatile organic matter in expiratory air according to claim 1, is characterized in that:
During sampling, the sealed valve A(4 at device two ends), sealed valve B(5) open, tester blows facing to gas blow pipe (1), person to be tested once exhales completely, namely after the initial and stage casing gas outflow of expiratory air, immediately by two ends sealed valve A(4), sealed valve B(5) shut, what now collect in sampling bottle (6) is end expiratory air.
5. the end gas sampling apparatus detected for volatile organic matter in expiratory air according to claim 1, is characterized in that:
The active volume of sampling bottle (6) is 20-100mL, when testee adopts normal exhalation mode, when once exhaling complete, and the end expiratory air of what sampling bottle (6) collected is testee.
6. the end gas sampling apparatus detected for volatile organic matter in expiratory air according to claim 1, is characterized in that:
Sample gas directly enters the mass spectrum injection port (7) of sampling bottle (6) upper end under carrier gas carrier band, carries out real-time analysis.
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| CN201310691210.5A CN104713750A (en) | 2013-12-13 | 2013-12-13 | End gas sampling apparatus for detecting volatile organic compounds in expired air |
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| CN201310691210.5A CN104713750A (en) | 2013-12-13 | 2013-12-13 | End gas sampling apparatus for detecting volatile organic compounds in expired air |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106501138A (en) * | 2015-09-06 | 2017-03-15 | 广州禾信分析仪器有限公司 | PM in expiratory air2.5Detection method and sample devices |
| CN106680043A (en) * | 2016-11-21 | 2017-05-17 | 北京蓝色星语科技有限公司 | Disturbance sampling device and disturbance sampling method of biochemical harmful factors |
| WO2018032465A1 (en) * | 2016-08-18 | 2018-02-22 | 深圳市先亚生物科技有限公司 | Device for collecting breath and aiding measurement of trace component in breath, and usage method thereof |
| CN108088712A (en) * | 2016-11-21 | 2018-05-29 | 中国科学院大连化学物理研究所 | A kind of expiratory air sampling apparatus and the method for sampling for the detection of direct mass spectrography |
| CN109839424A (en) * | 2017-11-27 | 2019-06-04 | 中国科学院大连化学物理研究所 | A kind of pre-treating method for the direct mass spectrography detection of pentane in expiratory air |
| CN111257399A (en) * | 2018-11-30 | 2020-06-09 | 中国科学院大连化学物理研究所 | Direct mass spectrum detection device for high-throughput measurement of volatile organic compounds in blood |
| CN114113283A (en) * | 2021-11-25 | 2022-03-01 | 成都艾立本科技有限公司 | Expired gas collection system and mass spectrometry device |
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Application publication date: 20150617 |