CN104705764A - Solid-liquid separation method for fermentation product - Google Patents
Solid-liquid separation method for fermentation product Download PDFInfo
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- CN104705764A CN104705764A CN201510100821.7A CN201510100821A CN104705764A CN 104705764 A CN104705764 A CN 104705764A CN 201510100821 A CN201510100821 A CN 201510100821A CN 104705764 A CN104705764 A CN 104705764A
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Abstract
The invention discloses a solid-liquid separation method for a fermentation product. The method comprises the following steps: performing low-temperature freezing treatment on the fermentation product, and performing unfreezing, filtering or centrifugation. According to the method, a freezing method is adopted for treating a liquid fermentation product of fungi, so that solid-liquid separation speed is obviously increased; in a freezing process, cells are broken, contents are released, and the active substance content and components of filtrate are increased; by low-temperature treatment, the activity of substances is prevented from being damaged.
Description
Technical field
The invention belongs to field of fermentation engineering, be specifically related to a kind of solid-liquid separating method of tunning.
Background technology
The tunning of part microorganism especially mushroom is a kind of gel slurries of complexity, and it comprises product, cell, mycelia, and other biological matter composition.The traditional solid-liquid separating method of edible fungus fermented product adopts methods such as directly filtering, refilter after heat pre-treatment.Weak point existing for this method of direct filtration is: directly filter in the filtrate of gained the outer active material of the born of the same parents be only secreted in zymotic fluid, and active material is not released in mycelium cell, therefore with the edible mushroom functional beverage that this method is produced, its polysaccharide isoreactivity content of material is on the low side.Refilter after heat pre-treatment and refer to that tunning is after boiling, cold filtration, mycelium in heating process in cytolysis born of the same parents active material be discharged in zymotic fluid, therefore the filtrate activity substance content arrived of this method is higher, but due to high-temperature boiling process, a lot of active material can be decomposed and reduce the function of beverage itself.In addition, due to slurries thickness, be difficult to be separated, so traditional filter method is generally consuming time longer, running cost is higher.
Summary of the invention
For the deficiency existing for prior art, the object of the present invention is to provide a kind of solid-liquid separating method of tunning.
The technical solution used in the present invention is:
A solid-liquid separating method for tunning, comprises the steps: after the process of tunning cryogenic freezing, melts, filtration or centrifugal.
As preferably, the process of described tunning cryogenic freezing refers to that first tunning being refrigerated to frozen water melts state altogether, is refrigerated to icing again after mechanical agitation fragmentation.
Described mechanical agitation break process, refers to and stir 1-5 minute under the mixing speed of 100-300 rev/min.
As preferably, described centrifugal condition is rotating speed 3000-5000 rev/min, centrifugation time 3-5min.
As preferably, in tunning, add appropriate Na
2cO
3be conducive to the Separation of Solid and Liquid of fermentate.Preferred further, Na
2cO
3interpolation concentration be 0.05-0.1 g/100mL.
Described tunning refers to the not segregative product with viscosity higher after microbial liquid fermentation.
Described microorganism comprises and is not limited to following microorganism: Ganoderma Lucidum, camphor tree sesame bacterium, manyzoned polypore bacteria, hericium erinaceus, cordyceps, white fungus bacterium, flat mushroom bacterium, schizochytrium limacinum etc.
The invention has the beneficial effects as follows:
Adopt the high viscosity liquid tunning of freeze-thaw method process microorganism, Separation of Solid and Liquid speed is obviously accelerated, and in frozen-thaw process, cell rupture, content discharges, and increases activity substance content and the composition of filtrate, and K cryogenic treatment ensures that species activity is not destroyed.
Accompanying drawing explanation
Fig. 1 is centrifugal effect figure after 4 kinds of freezing processing of embodiment 3;
Fig. 2 is embodiment 4 solid-liquid separation effect figure;
Fig. 3 is embodiment 4 solid-liquid separation effect figure.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated, but be not limited thereto.
embodiment 1
Prepared by tunning
Fermentation medium: glucose 50 g, peptone 3 g, corn flour 50 g, wheat bran 10 g, KH
2pO
41.5 g, MgSO
40.5 g adding distil water is settled to 1 L, is poured into respectively by the culture medium prepared in 250mL conical flask, often bottled 100 mL, and 121 DEG C of autoclavings 20 ~ 30 minutes, drop to after below 30 DEG C until temperature, for subsequent use.
Seed culture medium: potato 180 ~ 220 g, add 1L distilled water, boil about 30min, cross leaching juice, add glucose 20g, peptone 2-3g, KH2PO4 1-1.5 gram, MgSO4 0.5g, distilled water is settled to 1L, do not pour in 250mL conical flask, often bottled 100 mL, 121 DEG C of autoclavings 25 ~ 30 minutes.
Fermentation seed is cultivated: respectively glossy ganoderma, camphor tree sesame, rainbow conk, Hericium erinaceus, Cordyceps militaris, white fungus, flat mushroom are got 3-4 ferfas kind from culture dish flat-plate bacterial colony edge and be inoculated in seed culture medium, 25 DEG C, 150r/min, shaken cultivation 5 ~ 7 days.
Fermented and cultured: pressed by various nutrient solution in the inoculum concentration access fermentation medium of 10% respectively, in 25 DEG C, 150r/min, shaken cultivation 13 ~ 15 days under condition, obtains respective thick tunning.
embodiment 2
Tunning separation method 1
Isolated by filtration: each 200mL of tunning in case study on implementation 1 is placed in after-22 DEG C of refrigerator and cooled freeze, after melting under 35 DEG C of-55 DEG C of conditions, carries out suction filtration; Separately get the undressed tunning of each 200 mL of tunning in case study on implementation 1 in contrast.Carry out suction filtration according to a conventional method: the suction funnel of diameter 15 cm is connected on bottle,suction, suction funnel point mouth is away from bleeding point, and Medium speed filter paper puts into suction funnel, drips a small amount of water, allows filter paper be adjacent to, and adds and treats suction filtration sample, and suction filtration is to dry.
Centrifugation: get each 50 mL of case study on implementation 1 gained tunning, is placed in after-22 DEG C of refrigerator and cooled freeze, carries out centrifugation after melting under 35 DEG C ~ 55 DEG C conditions; Separately get and execute each 50 mL of undressed tunning in case 1 in contrast.Centrifugal condition: centrifugal 5min under 5000 r/min conditions.
Relatively freeze thawing treatment and the filtration time of untreated samples and the supernatant volume of centrifugal rear gained, result is as shown in table 1 below:
The relatively Contents of Main Components of freeze thawing treatment and untreated samples gained filtrate, result is as shown in table 2 below:
embodiment 3
Tunning separation method 2
Method 1: freezing rear mechanical agitation: get case study on implementation 1 gained glossy ganoderma fermentation product 50 mL, be placed in-22 DEG C of refrigerator and cooled to freeze, then melt 35 DEG C ~ 55 DEG C condition lower part, 1-5 minute is stirred again under the mixing speed of 100-300 rev/min, after continuing thawing, centrifugal 5min under 5000 r/min conditions.
Method 2: mechanical agitation under frozen water melts state altogether: get case study on implementation 1 gained glossy ganoderma fermentation product 50 mL, be placed in-22 DEG C of refrigerator and cooled to freeze the inside that to freeze to surface and do not freeze (frozen water melts state altogether), 1-5 minute is stirred under the mixing speed of 100-300 rev/min, melt under continuing at 35 DEG C ~ 55 DEG C conditions completely, centrifugal 5min under 5000 r/min conditions.
Method 3: freezing again after mechanical agitation under frozen water melts state altogether: get case study on implementation 1 gained glossy ganoderma fermentation product 50 mL, be placed in-22 DEG C of refrigerator and cooled to freeze to frozen water and melt state altogether, stir 1-5 minute under the mixing speed of 100-300 rev/min after, continue to freeze ice-22 DEG C of refrigerator and cooled, after melting under 35 DEG C of-55 DEG C of conditions again, centrifugal 5min under 5000 r/min conditions.
With in embodiment 2 through freeze thawing, without mechanical agitation process is contrast.Relatively active constituent content after 4 kinds of freezing processing, result is as shown in table 3 below, and after 4 kinds of freezing processing, centrifugal effect as shown in Figure 1.
embodiment 4
Get case study on implementation 1 gained glossy ganoderma fermentation product 2 parts, every part of each 50 mL, a interpolation 0.04g Na
2cO
3another part is not added, 2 increment product are processed with method 3 in embodiment 3, be placed in-22 DEG C of refrigerator and cooled to freeze the inside that to freeze to surface and do not freeze (frozen water melts state altogether), stir 1-5 minute under the mixing speed of 100-300 rev/min after, continue after-22 DEG C of refrigerator and cooled are frozen, melt under 35 DEG C ~ 55 DEG C conditions, centrifugal 3min under 3000 r/min conditions, with case study on implementation 1 gained glossy ganoderma fermentation product 50mL for contrast.Relatively solid-liquid separation effect between different disposal, result is illustrated in fig. 2 shown below.
Poured out by supernatant in Fig. 2, compare the supernatant clarity of different disposal sample, comparative result such as figure below institute 3 shows: add Na
2cO
3the supernatant of process compares clarification, does not add Na
2cO
3supernatant still visible part floccule.
Above embodiment shows, adopts process tunning of the present invention, and Separation of Solid and Liquid speed is obviously accelerated, and in frozen-thaw process, adopt mechanical agitation to make cell rupture abundant, content discharges, increase activity substance content and the composition of filtrate, K cryogenic treatment ensures that species activity is not destroyed.
Above embodiment is only introduces preferred case of the present invention, to those skilled in the art, not deviating from any apparent changes and improvements of carrying out in the scope of spirit of the present invention, all should be regarded as a part of the present invention.
Claims (8)
1. a solid-liquid separating method for tunning, comprises the steps: after the process of tunning cryogenic freezing, melts, filtration or centrifugal.
2. solid-liquid separating method according to claim 1, is characterized in that, the process of tunning cryogenic freezing refers to that first tunning being refrigerated to frozen water melts state altogether, is refrigerated to icing again after mechanical agitation fragmentation.
3. solid-liquid separating method according to claim 2, is characterized in that, described mechanical agitation break process, refers to stir 1-5 minute under the mixing speed of 100-300 rev/min.
4. solid-liquid separating method according to claim 1, is characterized in that, described centrifugal condition is rotating speed 3000-5000 rev/min, centrifugation time 3-5min.
5. solid-liquid separating method according to claim 1, is characterized in that, adds appropriate Na in tunning
2cO
3be conducive to the Separation of Solid and Liquid of fermentate.
6. solid-liquid separating method according to claim 5, is characterized in that, Na
2cO
3interpolation concentration be 0.05-0.1 g/100mL.
7. the solid-liquid separating method according to any one of claim 1-6, is characterized in that, described tunning refers to the not segregative product with viscosity higher after microbial liquid fermentation.
8. the solid-liquid separating method of tunning according to claim 7, it is characterized in that, described microorganism comprises and is not limited to following microorganism: Ganoderma Lucidum, camphor tree sesame bacterium, manyzoned polypore bacteria, hericium erinaceus, cordyceps, white fungus bacterium, flat mushroom bacterium, schizochytrium limacinum etc.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105725146A (en) * | 2016-02-06 | 2016-07-06 | 湖北工业大学 | Golden cordyceps sinensis and lotus root soup stock |
| CN105747046A (en) * | 2016-03-11 | 2016-07-13 | 辽宁隆维生物科技有限公司 | Cordyceps militaris liquid beverage and preparation method thereof |
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| CN105747046A (en) * | 2016-03-11 | 2016-07-13 | 辽宁隆维生物科技有限公司 | Cordyceps militaris liquid beverage and preparation method thereof |
| CN105747046B (en) * | 2016-03-11 | 2019-05-07 | 辽宁隆维生物科技有限公司 | A kind of Cordyceps militaris liquid drink and preparation method thereof |
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Address after: 510316 Haizhuqu District, Guangdong Province, No. 10, road Patentee after: GUANGDONG PROVINCIAL BIOENGINEERING INSTITUTE (GUANGZHOU SUGARCANE INDUSTRY RESEARCH INSTITUTE) Address before: 510316 Haizhuqu District, Guangdong Province, No. 10, road Patentee before: Guangzhou Sugarcane Industry Research Institute |
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Address after: 510316, 10, pomegranate Road, Guangzhou, Guangdong, Haizhuqu District Patentee after: Institute of bioengineering, Guangdong Academy of Sciences Address before: 510316, 10, pomegranate Road, Guangzhou, Guangdong, Haizhuqu District Patentee before: GUANGDONG PROVINCIAL BIOENGINEERING INSTITUTE (GUANGZHOU SUGARCANE INDUSTRY Research Institute) |
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Effective date of registration: 20201225 Address after: 510800 No. 88, Wynn Road, Xinhua Street, Huadu District, Guangzhou, Guangdong. Patentee after: GUANGZHOU OPSEVE COSMETICS Co.,Ltd. Address before: 510316, 10, pomegranate Road, Guangzhou, Guangdong, Haizhuqu District Patentee before: Institute of bioengineering, Guangdong Academy of Sciences |
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