CN104686682B - It is a kind of with probiotics fermention milk beverage relaxed bowel with auxiliary lipid-lowering function and preparation method thereof - Google Patents
It is a kind of with probiotics fermention milk beverage relaxed bowel with auxiliary lipid-lowering function and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of with probiotic beverage relaxed bowel with auxiliary lipid-lowering function and preparation method thereof.It is addition whey powder treatment fluid, beer, banana pulp, sweet potato paste, FOS, xylitol on the basis of skimmed milk enzymolysis liquid is obtained using trypsin hydrolysis recovery skimmed milk, addition hawthorn, the red sage root, root of kudzu vine medicinal plant extraction prepare mixed-culture medium；After thermally treated cooling, inoculation mixing probiotics leaven, what fermented 24 72h was prepared from, with the probiotic fermentation beverage relaxed bowel with auxiliary lipid-lowering function.Product of the present invention can significantly reduce TC, TG, LDL C content in rat blood serum, significantly rise HDL C and ApoAI contents；Rat blood serum ALT and AST level is significantly reduced, SOD vigor in liver organization is significantly improved, effectively alleviates the hepatic injury that lipid peroxidation is caused, is relaxed bowel and auxiliary lipid-lowering function with good.
Description
Technical field
The invention belongs to food processing field, particularly probiotic fermentation beverage functional food and preparation method.
Background technology
Hyperlipidemia is clinically common lipid-metabolism sexual disorder disease, be often referred to cholesterol in blood plasma (or) glycerine three
Ester is raised, and easily triggers the diseases such as coronary heart disease, atherosclerosis.Blood fat height has with diet intake and HypercholesterolemicRats
Substantial connection, in recent years, with the change of rhythm of life and diet structure, the hyperlipidemia incidence of disease is in steeply rise trend,
It is serious to threaten human health.
At present, in the market be usually used in treat hyperlipidemia Western medicine have Statins, fibrates etc., and Effects of Xuezhikang, lipid-loweringing
The Chinese patent drug such as spirit and Zhibituo.Although medicine lipid-loweringing achieves very big curative effect, but most fat-reducing medicaments are easily caused serum and turn ammonia
The adverse reaction such as enzyme rise and aching pain of muscles, and long-term taking is needed, it is costly.In recent years, with to traditional antilipemic Chinese herbal medicine
Research deepens continuously, it was demonstrated that traditional Chinese medicine has the advantage of uniqueness in terms of fat is adjusted, and is mainly reflected in multipath, Mutiple Targets, wide
Compose reducing blood lipid, in terms of Small side effects, it has also become the focus of medicines and health protection research.The lipid regulation that different health foods stress
Mode is different, can be divided into following three kinds according to the difference of the tune fat mode of auxiliary blood fat reducing health products, that is, reduces cholesterol, reduction
Triglycerides, raising inoxidizability.
In recent years, although there is research to confirm that human serum cholesterol can be reduced and contain by taking lactic acid bacteria and its correlated product
Amount, reduces cardiovascular disease incidence rate.But the related feature fermented dairy product of existing market is also extremely limited, it is basic former
Cause, it is single to realize that norcholesterol reaches auxiliary lipid-lowering effect by lactic acid bacteria, it is still extremely limited, in order to overcome drawbacks described above,
The difference in functionality characteristic of lactic acid bacteria is combined by we, and is combined with Chinese traditional herbs, realizes functional characteristic synergy, so as to open
Hair is with the probiotics fermention milk beverage relaxed bowel with auxiliary lipid-lowering function.
The content of the invention
On the basis of the present invention to traditional Chinese traditional medicines depressing lipid by screening, with the probiotic lactobacillus with reducing blood lipid ability
With reference to, display one's respective advantages jointly, develop with relax bowel and auxiliary lipid-lowering efficacy compound probiotic drinks.
A kind of preparation method with relaxing bowel with the probiotic beverage of auxiliary lipid-lowering function, is to utilize tryptose
Enzyme hydrolysis is restored on the basis of skimmed milk obtains skimmed milk enzymolysis liquid, addition whey powder treatment fluid, beer, banana pulp, sweet potato paste,
FOS, xylitol, medicinal plant extraction prepared by addition hawthorn, the red sage root, the root of kudzu vine, prepare mixed-culture medium；It is thermally treated cold
But after, inoculation mixing probiotics leaven, what fermented 24-72h was prepared from, with relaxing bowel and auxiliary lipid-lowering function
Probiotic fermentation beverage.
Present invention also offers the probiotic fermentation beverage prepared by the above method.
In the inventive method, whey powder processing liquid and preparation method thereof is as follows, and whey powder, which is dissolved in water, is made 5%-10% (w/w)
Whey powder solution, 1-3h is heated in 95 DEG C, and whey powder treatment fluid is made；Skimmed milk enzymolysis liquid and preparation method thereof is as follows, prepares 12%
(w/w) skimmed milk, 95 DEG C, solution total amount 0.6%-0.8% (w/w) trypsase is added in 1-5min heat treatments, in 50 DEG C of enzymolysis
6-8h, 95 DEG C, 3-5min destroy the enzyme treatments after reaction terminates；The preparation method of medicinal plant extraction is as follows：Hawthorn powder presses feed liquid with water
Than 1:8-10 (w/w) is mixed, and 30min is extracted at 80 DEG C；Kudzu-vine root powder presses solid-liquid ratio 1 with water:8-10 (w/w) is mixed, addition
0.05%-0.10% (w/w) alpha-amylase and 0.05%-0.10% (w/w) beta amylase, 65 DEG C of enzymolysis 2-3h；The red sage root
Powder presses solid-liquid ratio 1 with water:17-20 (w/w) is mixed, and soaks 10h, and 80 DEG C of water-bath heat carry 30min；Three kinds of leaching liquors press 1:1:1(w/
W) it is combined, after colloid mill, medicinal plant extraction is made in 120 mesh sieve net filtrations, standby in 4 DEG C of refrigerations after sterilization.
Probiotics leaven used is by Lactobacillus rhamnosus (L.rhamnosus) grx19, Lactobacillus helveticus in the present invention
(Lactobacillus helveticus) grx96, streptococcus thermophilus (S.thermopilus) grx02, bifidobacterium adolescentis
(B.adolescentis) grx066, lactobacillus acidophilus (L.acidophilus) grx95, Lactobacillus casei (L.casei)
Grx12 presses 1:1:1:1:1:Mixing probiotics leaven prepared by 1 (w/w) ratio.
Specifically method is：Whey powder treatment fluid is mixed with skimmed milk enzymolysis liquid, and adds medicinal plant extraction, is added simultaneously
Plus 3%-6% (w/w) banana pulp, 3%-6% (w/w) sweet potato paste, 3%-6% (w/w) 10-12 ° of P original wort concentration beer
Wine, 2%-4% (w/w) FOS, 2%-4% (w/w) xylitol, after sterilization cooling, inoculation 3%-5% (w/w) mixing
Probiotics leaven, in the 24-72h that fermented at 37 ± 1 DEG C, is prepared into probiotic fermentation beverage.
The present invention product compared with prior art, with advantages below：The present invention to traditional Chinese traditional medicines depressing lipid by carrying out
On the basis of screening, combined with the probiotic lactobacillus with reducing blood lipid ability, improve Chinese medicine to lipid metaboli key enzyme pancreas fat
The inhibiting rate of fat enzyme and HMG-CoA reductase；Play traditional Chinese medicine multipath, Mutiple Targets, wide spectrum reducing blood lipid, pair in terms of fat is adjusted
Effect is small to wait unique advantage, compares, and although medicine lipid-loweringing achieves very big curative effect, but most fat-reducing medicaments are easily caused blood
The adverse reaction such as clear transaminase rise and aching pain of muscles, compound probiotic drinks excellent flavor, functional characteristic is excellent, it is easy to
It is accepted；The blank in feature fermented dairy product market has been filled up, potentiality are had much.
Brief description of the drawings
Fig. 1 is normal rats liver tissue slices transmission electron microscope picture；
Fig. 2 is model group rats liver tissue slices transmission electron microscope picture；
Fig. 3 is medicine group rat liver tissue section transmission electron microscope picture；
Fig. 4 is mixed fermentation base-material group rat liver tissue section transmission electron microscope picture；
Fig. 5 is fermented beverage group rat liver tissue section transmission electron microscope picture of the present invention；
Fig. 6 is medicinal plant extraction+fermented beverage group rat liver tissue section transmission electron microscope picture；
Fig. 7 is blank run beverage group rat liver tissue section transmission electron microscope picture；
Fig. 8 is with the probiotic beverage preparation technology flow chart relaxed bowel with auxiliary lipid-lowering function.
Embodiment
1st, the present invention six kinds of probiotics used can be obtained by following approach：
(preserving number is CGMCCNO to Lactobacillus rhamnosus (Lactobacillus.rhamnosus) grx19：5519)：China
Microbiological Culture Collection administration committee common micro-organisms center；It is public in No. 201110441666.7 Chinese patent applications
Open；
(preserving number is CGMCCNO to streptococcus thermophilus (Streptococcus.thermopilus) grx02：2525)：China
Microbiological Culture Collection administration committee common micro-organisms center.Disclosed in ZL200810023012.0 Chinese patents.
(preserving number is CGMCCNO to bifidobacterium adolescentis (Bifidobacterium.adolescentis) grx066：
2406)；China Committee for Culture Collection of Microorganisms's common micro-organisms center.It is special in ZL200810123350.1 China
Disclosed in profit.
(preserving number is CGMCCNO to Lactobacillus casei (Lactobacillus.casei) grx12：7696)；Exist
201310312784.7 disclosed in number Chinese patent application；
Lactobacillus acidophilus (Lactobacillus.acidophilus) grx95：(preserving number is CGMCCNO：8875)：In
State's Microbiological Culture Collection administration committee common micro-organisms center.Disclosed in CN104059866A Chinese patents.
Lactobacillus helveticus (Lactobacillushelveticus) grx96：(preserving number is CGMCCNO：8876)：China
Microbiological Culture Collection administration committee common micro-organisms center.It is public in No. 201410270744.5 Chinese patent applications
Open.
2nd, the preparation of whey powder treatment fluid
The desalted whey powder of 70%-80% lactose contents is dissolved in water, 5%-10% (w/w) whey powder is prepared into molten
Liquid, 95 DEG C of heating 1-3h, is cooled down standby.
3rd, the preparation of skimmed milk enzymolysis liquid
Prepare 12% (w/w) and restore skimmed milk, 95 DEG C, skimmed milk total amount 0.6%-0.8% is restored in 5min heat treatments, addition
(w/w) trypsase, digests 6-8h at 50 DEG C, and reaction terminates latter 95 DEG C, and 3-5min destroy the enzyme treatments are cooled down standby.
4th, the preparation of fruit, vegetable juice
(1) banana processing mode
Banana is removed the peel, water blancing 8min color retentions, by material-water ratio 1：3 mashing, are cooled down standby after sterilization；
(2) sweet potato processing mode
Sweet potato is cleaned, material-water ratio 1:3 mashing, are cooled down standby after sterilization.
5th, the preparation of medicinal plant extraction
Patent of the present invention selects hawthorn, the red sage root and the taste integration of drinking and medicinal herbs Chinese herbal medicine of the root of kudzu vine three, and specific preparation technology is as follows：
Hawthorn powder presses solid-liquid ratio 1 with water:8-10 (w/w) is mixed, and 30min is extracted at 80 DEG C；Kudzu-vine root powder presses solid-liquid ratio with water
1:8-10 (w/w) is mixed, addition 0.05%-0.10% (w/w) alpha-amylase and 0.05%-0.10% (w/w) β-starch
Enzyme, 65 DEG C of enzymolysis 2-3h；Danshen powder presses solid-liquid ratio 1 with water:17-20 (w/w) is mixed, and soaks 10h, and 80 DEG C of water-bath heat carry 30min.
Three kinds of leaching liquors press 1:1:1 is combined, after colloid mill, 120 mesh sieve net filtrations, after sterilization, medicinal plant extraction is made, in 4 DEG C
Preservation is standby.
6th, prepared by leavening
Lactobacillus rhamnosus (L.rhamnosus) grx19, Lactobacillus helveticus (Lactobacillushelveticus)
Grx96, streptococcus thermophilus (S.thermopilus) grx02, bifidobacterium adolescentis (B.adolescentis) grx066, acidophilus
Lactobacillus (L.acidophilus) grx95, Lactobacillus casei (L.casei) grx12 press 1:1:1:1:1:It is prepared by 1 (w/w) ratio
Mixing probiotics leaven.
7th, sample preparation
(1) preparation of mixed fermentation base-material
Whey powder treatment fluid is mixed with skimmed milk enzymolysis liquid, 12% (w/w) medicinal plant extraction is added, and add 3%-
6% (w/w) banana pulp, 3%-6% (w/w) sweet potato paste, 3%-6% (w/w) beer, 2%-4% (w/w) oligomeric fruit
Sugar, 2%-4% (w/w) xylitol, is prepared into mixed fermentation base-material.
(2) preparation of fermented beverage of the present invention
Whey powder treatment fluid is mixed with skimmed milk enzymolysis liquid, 12% (w/w) medicinal plant extraction is added, and add 3%-
6% (w/w) banana pulp, 3%-6% (w/w) sweet potato paste, 3%-6% (w/w) 10-12 ° of P original wort concentration beer,
2%-4% (w/w) FOS, 2%-4% (w/w) xylitol, is prepared into mixed fermentation base-material.After sterilization cooling, inoculation
3%-5% mixing probiotics leavens, in the 24-72h that fermented at 37 ± 1 DEG C, after cooling, are prepared from probiotics fermention drink
Material.
(3) preparation of medicinal plant extraction+fermented beverage
The mixed fermentation liquid of medicinal plant extraction will be not added with, i.e., whey powder treatment fluid is mixed with skimmed milk enzymolysis liquid, and is added
Plus 3%-6% (w/w) banana pulp, 3%-6% (w/w) sweet potato paste, 3%-6% (w/w) 10-12 ° of P original wort concentration beer
Wine, 2%-4% (w/w) FOS, the mixed fermentation liquid that 2%-4% (w/w) xylitol is prepared into after sterilization cooling, connects
3%-5% (w/w) mixing probiotics leavens are planted, in after the 24-72h that fermented at 37 ± 1 DEG C, medicinal plant extraction are added, through cooling
Afterwards, it is prepared from.
(4) preparation of blank run beverage
The mixed fermentation liquid of medicinal plant extraction will be not added with, i.e., whey powder treatment fluid is mixed with skimmed milk enzymolysis liquid, and is added
Plus 3%-6% (w/w) banana pulp, 3%-6% (w/w) sweet potato paste, 3%-6% (w/w) 10-12 ° of P original wort concentration beer
Wine, 2%-4% (w/w) FOS, the mixed fermentation liquid that 2%-4% (w/w) xylitol is prepared into after sterilization cooling, connects
3%-5% (w/w) mixing probiotics leavens are planted, in the 24-72h that fermented at 37 ± 1 DEG C, after cooling, are prepared from.
8th, influence of the different samples to lipid metaboli key enzyme
Pancreatic lipases are the key enzymes of fat digestion absorption in meals, and hydrolyzable 50%-70% dietary fats are converted into
Glycerine and aliphatic acid, therefore the specificity of pancreatic lipases is suppressed effectively reduce digestion suction of the body to triglycerides
Receive.HMG-CoA reductase is the rate-limiting enzyme of Biosynthesis of cholesterol, by the activity for suppressing HMG-CoA reductase, it is possible to reduce
The generation of endogenous cholesterol, so as to play effect for reducing blood fat.By studying different samples to pancreatic lipases and HMG-CoA also
The influence of two lipid metaboli key enzymes of protoenzyme, adjustment effect of the Primary Study fermented beverage to lipid metaboli.
By four groups of samples described in 6, prepared using 1500 × g centrifugations 10min after supernatant, be respectively used to determine to pancreas fat
The influence of fat enzyme and HMG-CoA reductase vigor.
(1) the four kind of influence of sample to pancreatic lipases vigor
The influence result of study of sample prepared by above-mentioned four kinds of distinct methods to pancreatic lipases is as shown in table 1.
Inhibiting rate of the different experiments group of table 1 to pancreatic lipases vigor
Note：Abcde same columns compare, and different letters represent there is otherness (P<0.05)
As it can be seen from table 1 there is significant difference (P to the inhibiting rate of pancreatic lipases in each experimental group<0.05) it is, of the invention
Fermented beverage group is significantly higher than medicinal plant extraction+fermented beverage group, and pancreatic lipases can be suppressed by illustrating to contain in selected Chinese medicine
Active material, and Chinese medicine be metabolized through lactobacillus-fermented after can be lifted its effect activity；Blank run beverage group is to pancreas fat
The inhibiting rate of enzyme is relatively low, and only 3.1%, show that simple lactic acid bacteria metabolite is smaller to pancreatic lipases effect of vigor.
(2) the four kinds of influences of sample to HMG-CoA reductase vigor
Four kinds of samples are prepared according to 6 methods describeds, each reaction group is respectively added to according to table 2, utilizes high performance liquid chromatography
Instrument, which determines enzymatic reaction, to be terminated to be NADPH concentration in reaction system, calculates HMG-CoA reductase inhibiting rate, concrete outcome such as table 3
It is shown.
Constituent in the reaction system of table 2
Inhibiting rate of the different experiments group of table 3 to HMG-CoA reductase activity
Note：Abcde same columns compare, and different letters represent there is otherness (P<0.05)
From table 3 it can be seen that different experiments group differs greatly to HMG-CoA reductase activity influence, wherein present invention hair
Ferment beverage group is most strong to HMG-CoA reductase rejection ability, and inhibiting rate reaches 52.67%；And blank run beverage group is to HMG-
The performance of CoA reductase activities is faint to be suppressed.
9th, auxiliary lipid-lowering efficacy characteristic research in different sample bodies
(1) foundation of hyperlipemia model of rats
The healthy male Wistar rats of 70 150~180g are taken, is made with basal feed after the raising of 3d adaptability, is randomly divided into
Control group (10), hyperlipidemia model group (60).Control group fed basal feed, remaining 60 all feed high lipid food and make height
Fat model, during raising, records food-intake, weight is weighed weekly daily.After two weeks, control group and hyperlipidemia model group take respectively
Three, fasting 14h is plucked eyeball blood sampling, is determined rat blood serum T-CHOL (TC), triglycerides respectively using Biochemical Analyzer
(TG), the index such as low-density lipoprotein (LDL-C), HDL (HDL-C).As a result it is as shown in table 4.
Table 4 feeds normal diet rat and high lipid food rat fat change (x ± sd, n=3)
Note：Ab same columns compare, and different letters represent there is otherness (P<0.05)
Compared with normal group, hyperlipidemia model group rat TC, TG, LDL-C significantly raise (P<0.05), HDL-C is significantly reduced
(P<0.05), illustrate to be successfully established olive Antihyperlipidemia capsule.
(2) animal packet and processing
Gavage experiment is carried out after modeling success, the time is 4 weeks.Gavage is carried out according to table 5, wherein sample preparation is according in 6
Methods described, medicine group gavage medicine preparation method is to take 10mg Effects of Xuezhikang dry powder to be dissolved in 1mL physiological saline.Timing supplement drink
Water and feed, change bedding and padding, carry out Continuous Observation after the completion of gavage to animal, poisoning manifestations of timely recording laboratory animal and dead
Situation is died, it is dissected if necessary and makees naked eyes pathological observation.Last can't help water 14h to fasting after sample, pluck eyeball collection
Blood, solution takes liver, spleen, kidney and heart etc. and determines index of correlation.
The experiment rat packet of table 5 and gavage
Note：Effects of Xuezhikang adult is daily：Consumption 10mg/kg
(3) to the influence of hyperlipidemia ratses weight and organ index
Experimental stage, the tested Hair of Rat coloured light of each group is clean, and appetite is normal, and mental alertness does not occur abnormal sign or death
Phenomenon.Rat is dissected in off-test, takes liver, heart, kidney and spleen, calculates each group Rats Organs and Tissues index, as shown in table 6.
Influence (x ± sd, n=8) of the different experiments group of table 6 to Rats Organs and Tissues index
Note：Abc same columns compare, and different letters represent there is otherness (P<0.05)
As can be seen from Table 6, each gavage group is compared with normal group, liver index there was no significant difference (P>0.05), model
The liver index of group has significant difference (P compared to normal group<0.05)；Compared with normal control, each gavage group and model
The kidney of group, the organ index of heart and spleen there are no significant difference (P>0.05).
Result above, illustrating the nursing of high lipid food can be such that the lipids such as cholesterol are accumulated on liver, cause liver weight
Amount increase, can effectively reduce liver lipids content, so that liver mass is reduced after the related fermented beverage of gavage；Together
When, gavage group renal index compared with normal group is without exception, illustrates to be free of to the poisonous pair of rat in selected Chinese medicine and fermented beverage
The material of effect.
(4) to the influence of rat fat
The index such as TC, TG, LDL-C and HDL-C in rat blood serum is determined, concrete outcome is as follows.
Influence (x ± sd, n=8) of the different experiments group of table 7 to Serum Lipids in Experimental HypercholesterolemicRats
Note：Abcde same columns compare, and different letters represent there is otherness (P<0.05)
As seen from Table 7, compared with model group, gavage group Serum TC and TG are decreased significantly (P<0.05), wherein
Fermented beverage group TC of the present invention declines that degree is especially pronounced, there was no significant difference between medicine group (P>0.05)；With model group phase
Than in addition to mixed fermentation base-material group, remaining gavage group rat blood serum LDL-C significantly reduces (P<0.05)；Compared with model group, respectively
HDL-C has a certain degree of rise, but the not notable (P of difference in gavage group serum>0.05).
(5) to the influence of rat blood serum Apolipoprotein Levels
Take 300uL serum using immune transmission nephelometry ApoAI, ApoB, concrete outcome is as follows.
ApoAI and ApoB contents (x ± sd, n=8) in the rat blood serum of table 8
Note：Abcd same columns compare, and different letters represent there is otherness (P<0.05)
As can be seen from Table 8, model group rats serum ApoAI content is substantially less than normal group (P<0.05)；With model
Group is compared, and gavage group ApoAI contents significantly raise (P<0.05), and fermented beverage group of the present invention compare medicinal plant extraction+fermentation
Significant difference (P is not present in beverage group>0.05)；Compared with normal group, the serum ApoB values of model group have to be raised to a certain degree,
But not notable (the P of difference>0.05), each gavage group is compared with model group, and serum ApoB values are declined slightly, and difference is not notable, therefore
In the absence of statistical significance.
(6) hyperlipemia rat serum alt and AST are influenceed
Rat blood serum is prepared, Serum ALT and AST is determined, concrete outcome is as shown in table 9.
Rat blood serum ALT and AST the index determining result (x ± sd, n=8) of table 9
Note：Abcd same columns compare, and different letters represent there is otherness (P<0.05)
As can be seen from Table 9, compared with normal group, high blood lipid model group rat blood serum ALT, AST level is significantly raised
(P<0.05)；Compared with model group, except blank run beverage group ALT differences are not significantly outer, remaining gavage group Serum ALT with
AST levels are remarkably decreased (P<0.05).(7) to the influence of Antioxidation Ability of Rats
This experiment determines antioxidase SOD activity, concrete outcome such as table in the tested rat blood serum of each group and liver organization
Shown in 10.
Antioxidase SOD activity in the rat blood serum of table 10 and liver organization
Note：Abcd same columns compare, and different letters represent there is otherness (P<0.05)
It can be seen from Table 10 that, compared with normal group, model group serum activity of SOD and SOD in liver are notable
Decline (P<0.05)；Compared with model group, each gavage group serum and SOD in liver significant difference (P<0.05).
(8) cholesterol TC contents in rat liver homogenate
Cholesterol TC in rat liver homogenate is prepared, using Biochemical Analyzer, TC contents in each group rat liver homogenate, knot is determined
Fruit is as shown in table 11.
The measurement result (x ± sd, n=8) of TC contents in the rat liver homogenate of table 11
Note：Abcd same columns compare, and different letters represent there is otherness (P<0.05)
By table 11 as can be seen that compared with normal group, the cholesterol level in model group rats liver significantly raises (P<
0.05)；Compared with model group, each gavage group rats'liver inner cholesterol content has different degrees of decline.
(9) cholesterol and Determination of Bile Acids in rat excrement
The measurement result (x ± sd, n=8) of cholesterol (TC) and bile acid (TBA) content in the rat excrement of table 12
Note：Abcde same columns compare, and different letters represent there is otherness (P<0.05)
As can be seen from the above table, the cholesterol and Determination of Bile Acids in hyperlipidemia model excrement are all remarkably higher than normal group (P<
0.05)；Compared to model group, in addition to mixed fermentation base-material group, cholesterol and Determination of Bile Acids have aobvious in remaining gavage group excrement
Write rise (P<0.05)；There is significant difference (P between fermented beverage group of the present invention and medicinal plant extraction+fermented beverage group<
0.05)。
(10) liver tissue slices are observed
As shown in figure 1, fat-free denaturation in normal group liver organization, the overall structure of liver cell is normal.
As shown in Fig. 2 steatosis, visible a large amount of bubbles or particle in liver cell kytoplasm occur for the liver cell of model group
Shape lipid vacuole, density granule is fatty to steep substantially more than normal group all than larger.
As shown in Figure 3 and Figure 5, under identical multiplication factor, fermented beverage group of the present invention and medicine group can substantially be found
Liver cell in the size and number of lipochondrion be decreased obviously, almost close to normal group；
As shown in Figure 6 and Figure 7, in blank run beverage group and medicinal plant extraction+fermented beverage group rat liver cells
Fat vacuole quantity has been reduced, but still has a small amount of larger lipid vacuole to occur；
As shown in figure 4, being distributed more lipid vacuole in mixed fermentation base-material group liver organization, there is Minimal change phenomenon.
Embodiment
Following examples illustrate practice and the presently preferred embodiment of the present invention.
However, it is understood that person skilled in the art can be in spirit of the invention after in view of present disclosure
With change is made in scope.
Embodiment 1
Flow such as Fig. 8：10% (w/w) whey powder solution is made in the dissolving of 6.7kg whey powder+60kg water, and 3h is heated in 95 DEG C,
66.7kg whey powder treatment fluids are made.6kg skimmed milk power+44kg water, which dissolves, is made 12% (w/w) recovery skimmed milks, 95 DEG C,
5min is heat-treated, and is cooled to 50 DEG C, adds 300g (solution total amount 0.6%) trypsase, 8h is digested in 50 DEG C, after reaction terminates
95 DEG C, 50kg skimmed milk enzymolysis liquids are made in 3min destroy the enzyme treatments.Hawthorn powder 1kg+9kg water (hawthorn powder and water solid-liquid ratio 1:9) mix
Close, in extracting 30min at 80 DEG C；Kudzu-vine root powder 1kg+9kg water (kudzu-vine root powder and water solid-liquid ratio 1:9) mix, add 8g (solution total amounts
0.08%) alpha-amylase and 8g (the 0.08% of solution total amount) beta amylase, digest 2h at 65 DEG C；Danshen powder 0.5kg+
9.5kg water (danshen powder and water solid-liquid ratio 1:19) mix, after immersion 10h, in extracting 30min at 80 DEG C；Three kinds of Chinese medicines are handled
Through colloid mill after liquid mixing, medicinal plant extraction 24kg is made in 120 mesh sieve net filtrations.By 66.7kg whey powders treatment fluid and 50kg
Skimmed milk enzymolysis liquid is mixed, and adds medicinal plant extraction 24kg, 10-12 ° of P original wort concentration beer 10kg, banana pulp 10kg, sweet potato
10kg, FOS 4kg, xylitol 4kg, pectin 0.6kg, water 20.7kg are starched, is mixed, is heat-treated (90-95 DEG C, 15min), it is cold
But to 37 DEG C or so, the mixing of access 4% probiotics leaven (L.rhamnosusgrx19,
Lactobacillushelveticusgrx96、S.thermopilusgrx02、B.adolescentisgrx066、
L.acidophilusgrx95, L.caseigrx12 press 1:1:1:1:1:1 (w/w) ratio is prepared from), 37 DEG C of ferment at constant temperature
40h, homogeneous (20MPa) is filling, and 200kg probiotic beverages are made.
Embodiment 2
Flow such as Fig. 8：8% (w/w) whey powder solution is made in the dissolving of 5.3kg whey powder+61.4kg water, and 95 DEG C are heated 2h,
It is prepared into 66.7kg whey powder treatment fluids.6kg skimmed milk power+44kg water, which dissolves, is made 12% (w/w) recovery skimmed milks, 95 DEG C,
5min is heat-treated, and is cooled to 50 DEG C, adds 400g (solution total amount 0.8%) trypsase, and 50 DEG C of enzymolysis 6h, reaction terminates rear 95
DEG C, 5min destroy the enzyme treatments are prepared into 50kg skimmed milk enzymolysis liquids.Hawthorn powder 1kg+9kg water (hawthorn powder and water solid-liquid ratio 1:9) mix
Close, in extracting 30min at 80 DEG C；Kudzu-vine root powder 1kg+9kg water (kudzu-vine root powder and water solid-liquid ratio 1:9) mix, add 5g (solution total amounts
0.05%) alpha-amylase and 5g (the 0.05% of solution total amount) beta amylase, digest 3h at 65 DEG C；Danshen powder 0.5kg+
9.5kg water (danshen powder and water solid-liquid ratio 1:19) mix, after immersion 10h, in extracting 30min at 80 DEG C；Three kinds of Chinese medicines are handled
Through colloid mill after liquid mixing, medicinal plant extraction 24kg is made in 120 mesh sieve net filtrations.By 66.7kg whey powders treatment fluid and 50kg
Skimmed milk enzymolysis liquid is mixed, and adds medicinal plant extraction 24kg, 10-12 ° of P original wort concentration beer 8kg, banana pulp 8kg, sweet potato pastes
8kg, FOS 6kg, xylitol 6kg, pectin 0.6kg, water 22.7kg, are mixed, and are heat-treated (90-95 DEG C, 15min), cooling
To 37 DEG C or so, the mixing of access 3% probiotics leaven (L.rhamnosusgrx19,
Lactobacillushelveticusgrx96、S.thermopilusgrx02、B.adolescentisgrx066、
L.acidophilus grx95, L.caseigrx12 press 1:1:1:1:1:1 (w/w) ratio is prepared from), 37 DEG C of ferment at constant temperature
50h, homogeneous (20MPa) is filling, and 200kg probiotic beverages are made.
Claims (3)
1. it is a kind of with the probiotic beverage relaxed bowel with auxiliary lipid-lowering function, it is characterised in that to pass through following method systems
It is standby：On the basis of skimmed milk enzymolysis liquid is obtained using trypsin hydrolysis recovery skimmed milk, addition whey powder treatment fluid, 3%-
6% (w/w) 10-12 ° of P original wort concentrations beer, 3%-6% (w/w) banana pulp, 3%-6% (w/w) sweet potato paste, 2%-4%
(w/w) FOS, 2%-4% (w/w) xylitol, medicinal plant extraction prepared by addition hawthorn, the red sage root, the root of kudzu vine, prepare mixing
Nutrient solution；After thermally treated cooling, inoculation 3%-5% (w/w) mixing probiotics leavens, in fermented 24- at 37 ± 1 DEG C
What 72h was prepared from, with the probiotic fermentation beverage relaxed bowel with auxiliary lipid-lowering function.
2. a kind of preparation method with relaxing bowel with the probiotic beverage of auxiliary lipid-lowering function, it is characterised in that：In profit
On the basis of skimmed milk enzymolysis liquid being obtained with trypsin hydrolysis recovery skimmed milk, addition whey powder treatment fluid, 3%-6% (w/
W) 10-12 ° of P original wort concentrations beer, 3%-6% (w/w) banana pulp, 3%-6% (w/w) sweet potato paste, 2%-4% (w/w)
FOS, 2%-4% (w/w) xylitol, medicinal plant extraction prepared by addition hawthorn, the red sage root, the root of kudzu vine, prepare mixed culture
Liquid；After thermally treated cooling, inoculation 3%-5% (w/w) mixing probiotics leavens, through in 24-72h systems of being fermented at 37 ± 1 DEG C
It is standby to form；
Wherein：Whey powder processing liquid and preparation method thereof is as follows, and whey powder, which is dissolved in water, is made 5%-10% (w/w) whey powder solution,
1-3h is heated in 95 DEG C, whey powder treatment fluid is made；
Skimmed milk enzymolysis liquid and preparation method thereof is as follows, prepares 12% (w/w) skimmed milk, 95 DEG C, 1-5min heat treatments, addition solution is total
0.6%-0.8% (w/w) trypsase is measured, 6-8h, 95 DEG C, 3-5min destroy the enzyme treatments after reaction terminates are digested at 50 DEG C；
The preparation method of medicinal plant extraction is as follows：Hawthorn powder presses solid-liquid ratio 1 with water:8-10 (w/w) is mixed, and is extracted at 80 DEG C
30min；Kudzu-vine root powder presses solid-liquid ratio 1 with water:8-10 (w/w) mix, addition 0.05%-0.10% (w/w) alpha-amylase and
0.05%-0.10% (w/w) beta amylase, 65 DEG C of enzymolysis 2-3h；Danshen powder presses solid-liquid ratio 1 with water:17-20 (w/w) is mixed,
10h is soaked, 80 DEG C of water-bath heat carry 30min；Three kinds of leaching liquors press 1:1:1 (w/w) is combined, after colloid mill, 120 eye mesh screen mistakes
Filter, is made medicinal plant extraction, standby in 4 DEG C of refrigerations after sterilization.
3. preparation method according to claim 2, it is characterised in that probiotics leaven used is by Lactobacillus rhamnosus
(L.rhamnosus)CGMCC NO：5519th, Lactobacillus helveticus (Lactobacillus helveticus) CGMCC NO：8876、
Streptococcus thermophilus (S.thermopilus) CGMCC NO：2525th, bifidobacterium adolescentis (B.adolescentis) CGMCC NO：
2406th, lactobacillus acidophilus (L.acidophilus) CGMCC NO：8875th, Lactobacillus casei (L.casei) CGMCC NO：7696
By 1:1:1:1:1:Mixing probiotics leaven prepared by 1 (w/w) ratio.
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| CN109430380A (en) * | 2018-12-10 | 2019-03-08 | 扬州大学 | A kind of source of people mixing lactic acid bacteria acidified milk and preparation method thereof reducing cholesterol effect with auxiliary |
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| CN114717129B (en) * | 2021-08-23 | 2023-11-21 | 青岛蔚蓝生物股份有限公司 | Lactobacillus rhamnosus and application thereof in preventing and relieving constipation symptoms |
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