CN104650175A - Purification method of saponin - Google Patents
Purification method of saponin Download PDFInfo
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- CN104650175A CN104650175A CN201410260083.8A CN201410260083A CN104650175A CN 104650175 A CN104650175 A CN 104650175A CN 201410260083 A CN201410260083 A CN 201410260083A CN 104650175 A CN104650175 A CN 104650175A
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- CN
- China
- Prior art keywords
- saponin
- distillation
- concentration
- ethanolic soln
- add
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J75/00—Processes for the preparation of steroids in general
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Steroid Compounds (AREA)
Abstract
The invention discloses a purification method of saponin, and relates to the technical field of purification of products. The method comprises the following steps: adding water and dissolving a saponin crude product and turning into a macroporous adsorption resin to be adsorbed; adding distilled water and eluting and removing impurities; adding ethanol solutions with the concentration of 50 percent and ethanol solutions with the concentration of 55 percent respectively and eluting until the reaction of an antimony trichloride chloroform saturated solution is negative; collecting an elution liquid; performing pressure reduction and distillation; collecting distilled residues; and drying in vacuum to obtain refined saponin. The obtained saponin is high in biological activity and relatively high in purity.
Description
Technical field
The present invention relates to product purification techniques field, especially a kind of purification process of saponin(e.
Background technology
Saponin(e, also known as alkali soap body, saponin, Saponin/TSM etc.One class glucosides of saponin(e to be aglycon be triterpene or spirostane compounds, is mainly distributed in the higher plant of land, is also present in the marine organisms such as starfish and sea cucumber on a small quantity.The principle active component of many herbal medicine as ginseng, polygala root, balloonflower root, Radix Glycyrrhizae, the wind-weed and radix bupleuri etc. all contains saponin(e, and saponin(e has haemolysis, decreasing cholesterol, anti-inflammatory, antineoplastic effect.
At present, the purification process of saponin(e mainly includes machine solvent precipitation, n-butanol extraction, acid and alkali hydrolysis-extraction into ethyl acetate method etc., these methods not only use a large amount of acid-base solutions in purge process consumption, cause cost higher, and prepared saponin(e purity are lower.
Summary of the invention
The object of this invention is to provide a kind of purification process of saponin(e, the purification process of this saponin(e can solve the low problem of saponin(e purity.
In order to solve the problem, the technical solution used in the present invention is: the purification process of this saponin(e, comprises the following steps:
A, get saponin(e crude product, be dissolved in water, proceed to macroporous adsorptive resins, adsorb 1 hour ~ 1.5 hours;
B, add the distilled water of column volume 2 times ~ 2.5 times, wash-out removing impurity;
C, respectively add concentration be 50% ethanolic soln and 55% ethanolic soln be eluted to butter of antimony chloroform saturated solution reaction be negative;
D, collection elutriant, underpressure distillation, collects the rear residue of distillation, vacuum-drying.
Owing to have employed technique scheme, the present invention compared with prior art has following beneficial effect:
Adopt Purified saponins of the present invention, agents useful for same is few, and cost is low, and the saponin(e biological activity obtained is good, and purity is high, has larger market potential and wide application prospect.
Embodiment
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1
Get 2L water, add 10 kilograms of saponin(e crude products wherein, dissolve, then proceed to macroporous adsorptive resins, adsorb 1 hour; Add 2L distilled water, wash-out removing impurity; Add respectively again concentration be 50% ethanolic soln and 55% ethanolic soln be eluted to butter of antimony chloroform saturated solution reaction be negative; Collect elutriant, underpressure distillation, collect the rear residue of distillation, vacuum-drying, obtains refining saponin(e.
The present embodiment gained saponin(e biological activity is good, and its purity is 96.2%, has higher using value.
Embodiment 2
Get 4.2L water, add 20 kilograms of saponin(e crude products wherein, dissolve, then proceed to macroporous adsorptive resins, adsorb 1.5 hours; Add 2.5L distilled water, wash-out removing impurity; Add respectively again concentration be 50% ethanolic soln and 55% ethanolic soln be eluted to butter of antimony chloroform saturated solution reaction be negative; Collect elutriant, underpressure distillation, collect the rear residue of distillation, vacuum-drying, obtains refining saponin(e.
The present embodiment gained saponin(e biological activity is good, and its purity is 97.1%, has higher using value.
Embodiment 3
Get 2.8L water, add 15 kilograms of saponin(e crude products wherein, dissolve, then proceed to macroporous adsorptive resins, adsorb 1.2 hours; Add 2.3L distilled water, wash-out removing impurity; Add respectively again concentration be 50% ethanolic soln and 55% ethanolic soln be eluted to butter of antimony chloroform saturated solution reaction be negative; Collect elutriant, underpressure distillation, collect the rear residue of distillation, vacuum-drying, obtains refining saponin(e.
The present embodiment gained saponin(e biological activity is good, and its purity is 97.7%, has higher using value.
Claims (1)
1. a purification process for saponin(e, is characterized in that comprising the following steps:
A, get saponin(e crude product, be dissolved in water, proceed to macroporous adsorptive resins, adsorb 1 hour ~ 1.5 hours;
B, add the distilled water of column volume 2 times ~ 2.5 times, wash-out removing impurity;
C, respectively add concentration be 50% ethanolic soln and 55% ethanolic soln be eluted to butter of antimony chloroform saturated solution reaction be negative;
D, collection elutriant, underpressure distillation, collects the rear residue of distillation, vacuum-drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410260083.8A CN104650175A (en) | 2014-06-12 | 2014-06-12 | Purification method of saponin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410260083.8A CN104650175A (en) | 2014-06-12 | 2014-06-12 | Purification method of saponin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104650175A true CN104650175A (en) | 2015-05-27 |
Family
ID=53241861
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410260083.8A Pending CN104650175A (en) | 2014-06-12 | 2014-06-12 | Purification method of saponin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104650175A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117899142A (en) * | 2023-12-29 | 2024-04-19 | 唐宁医药科技(济南)有限公司 | Extraction preparation method for obtaining high-content polygala tenuifolia total saponins |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102670680A (en) * | 2012-05-26 | 2012-09-19 | 陇东学院 | Method for extracting total saponins from bark of acanthopanax brachypus harms |
-
2014
- 2014-06-12 CN CN201410260083.8A patent/CN104650175A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102670680A (en) * | 2012-05-26 | 2012-09-19 | 陇东学院 | Method for extracting total saponins from bark of acanthopanax brachypus harms |
Non-Patent Citations (2)
| Title |
|---|
| 刘中秋等: "大孔吸附树脂富集纯化毛冬青总皂苷工艺研究", 《中药新药与临床药理》 * |
| 蔡雄等: "大孔吸附树脂富集纯化人参总皂苷工艺", 《中成药》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117899142A (en) * | 2023-12-29 | 2024-04-19 | 唐宁医药科技(济南)有限公司 | Extraction preparation method for obtaining high-content polygala tenuifolia total saponins |
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Application publication date: 20150527 |
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| RJ01 | Rejection of invention patent application after publication |