CN104644533A - Preparation method of feminine care lotion - Google Patents
Preparation method of feminine care lotion Download PDFInfo
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- CN104644533A CN104644533A CN201310601058.7A CN201310601058A CN104644533A CN 104644533 A CN104644533 A CN 104644533A CN 201310601058 A CN201310601058 A CN 201310601058A CN 104644533 A CN104644533 A CN 104644533A
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Abstract
The invention relates to the technical fields of research and development of feminine care lotions and particularly relates to the technical field of preparation of a feminine vagina-cleaning care lotion. A preparation method of the feminine care lotion is characterized in that a preparation method of culture and breeding sieve of Lactobacillus delbrueckii (DM8909) and a care lotion of Lactobacillus delbrueckii is provided. The feminine care lotion has the beneficial effects that raw materials are innocuous and unpoisonous, the three wastes are not discharged basically, adverse environment influence is basically avoided, the biotechnical content is high, the production process is simple, the large-scale production is easily realized, and the upgrade of feminine disposable care products is improved; the feminine care lotion is capable of killing harmful bacteria in feminine vaginas, protecting and promoting the growth and breeding of beneficial bacteria, restoring normal acid environments in the feminine vaginas, maintaining the balance of florae in the feminine vaginas and rapidly eliminating the infection of various gynecological diseases.
Description
Technical field
What this patent related to is the technical field that female gynecological conditioning liquid is researched and developed, and what be specifically related to is the technical field that the clean cloudy conditioning liquid of a kind of women makes.
Background technology
At present because women is because of some factors vary, as sexual life, menstruation, childbirth, in the vagina that gynecilogical operation, inappropriate clean cloudy mode, immunity degradation etc. cause, the destruction of microecological balance, makes harmful bacteria excessive multiplication, and the various gynaecopathias caused infect.
The invention of this patent is based upon in the theoretical basis of microecology and Disinfection.Microecology is the new branch of science that Two decades years is set up and grown up, and it is " the life sciences branch of research normal microflora and its host's mutual relation ".Microecology illustrates the substantial connection between the survival and development of the mankind and the microorganism of its coexistence, it discloses all one's life of people all the time with of a great variety, and the microorganism of enormous amount together together.The total cellular score that data shows an adult is 1014, and in body, the quantity of normal flora is 1013, and because this person is not an isolated people, people is the homobium of himself and microorganism.Research shows, these microorganisms are related to the growth of human body, growth, health, disease just, and under normal circumstances, the microorganism that people carries with it interacts, and benefits mutually, restricts each other, reciprocal causation.But this normal balance is once destroy, and the beneficial flora in human body will obviously reduce, and body will functional disorder, and body constitution declines, and is eaten up with illness, therefore scientist point out human body beneficial flora number, whether to balance be the barometer of health.Make human body keep microecological balance to be just able to bacterium and mend bacterium; fungus treatment because human body due to self, environment, medicine, in the body of people, microecological balance often can be lacked of proper care; must make it to restore balance from extraneous probiotic supplemented or probiotics factor at any time, improve health.Based on above-mentioned theory, Tiny ecosystem health care just worldwide forms upsurge, and the ecological agent thus in ecological engineering industry arises at the historic moment, flourish.
Normal healthy women, vagina has nature defense function due to the intrusion of feature to pathogen of anatomical tissue.As closing of vaginal orifice, vagina front and rear wall is close to, the hypertrophy of vaginal epithelial cell under estrogenic impact, and cells of superficial layer keratinization, vaginal pH remains on pH4-5, the breeding of the pathogen of adaptation alkalescence is suppressed, and neck tube mucus is in alkalescence, the breeding of the pathogen of adaptation sour environment is suppressed, when the natural defense function of vagina is damaged, pathogen is easy to invade, and causes vaginal infection disease occurs.Youngest daughter and postmenopausal women are more vulnerable to infection because vagina epithelium is very thin.The antibacterial parasitized in healthy women vagina comprises gram-positive aerobic bacteria, and as lactobacillus, corynebacterium, anhemolytic streptococcus, enterococcus, epidermis glucose coccus, gram-negative aerobic bacteria has escherichia coli, Gardnerella.Anaerobe comprises clostruidium, peptostreptococcus, bacteroid and fusiform bacilarmature etc., still has Mobiluncns bacterium, mycoplasma and candidiasis etc. in addition.In these normal vagina flora, preponderate with lactobacillus, Vaginal lactobacilli plays pivotal role to maintenance vagina normal flora.The glycogen of vagina epithelium becomes lactic acid through the action breaks of lactobacillus, makes vagina local in weakly acidic condition, suppresses other bacterial parasite undue growth.In addition part lactobacillus still can synthesize hydrogen peroxide, and the positive lactobacilluss of these hydrogen peroxide are by suppressing other antibacterials with other oxide synergy.The sour environment that lactobacillus causes on the other hand is conducive to again reducing negatively charged cell surface and removing the glycosyl being covered in receptor surface, exposes receptor and contributes to bacterial adhesion.Therefore lactobacillus is the flora being regulated normal vagina by the pros and cons.But as aforementioned, when application some drugs is as broad-spectrum antibiotic, cancer therapy drug and immunosuppressant, or some infects as gonorrhea, syphilis, acquired immune deficiency syndrome (AIDS), or the impact such as gonadal hormone, and the balance of vaginal ecosystem all can be caused to destroy.Also can cause the generation of vaginal infection disease, normal with antibiotic therapy clinically, antibiotic application itself can cause dysbacteriosis, causes this sick protracted course of disease, brings great pain to patient.From the angle of microecology; therapy is adjusted by ecological agent; the composition of righting and the intravaginal normal flora of protection and ratio, recover the ability that it naturally resists foreign bacteria invasion, promotes that the self purification of itself is a kind of inexorable trend of this type of disease for the treatment of.
In sum, a kind of Feminine care solution of patent of the present invention based on ecological conditioning liquid theory and research and develop.Current ecological agent at present in the world research report be lactobacillus mixture prepared by 4 kinds of lactobacilluss such as DM8909, L.rh GR1 and L fem RC14, DM8909 but seem common conceptual phase at present.
Summary of the invention
In order to solve the various gynaecopathia difficult problem of women, solve the misery puzzlement that women brings because suffering from gynaecopathia, also women is in health, this patent provides the ecological conditioning liquid of a kind of new women and processing technology thereof, this washing liquid utilizes animal nutrition to cultivate in laboratory research Deshi Lactobacillus (DM8909) strain that screening effectively can treat female gynecological disease, Deshi Lactobacillus (DM8909) is being carried out to the benchmark mother solution of biofermentation breeding cultivation preparation development, and then development is used for the cleaning and nursing product at women's secret place.A manufacture method for the clean cloudy conditioning liquid of women, is characterized in that the cultivation breeding of Deshi Lactobacillus (DM8909) and the manufacture method of conditioning liquid thereof.
Described Deshi Lactobacillus (DM8909) cultivation breeding, by probiotics Deshi Lactobacillus (DM8909) bacterial strain seed inclined plane inoculating after seed bottle 37 DEG C cultivate 48 hours; Be inoculated in shake-flask seed culture medium, cultivate 48 hours for 37 DEG C; Shake-flask seed is being inoculated in fermentor cultivation 24 hours, centrifugalize, is obtaining fermented supernatant fluid, adding Deshi Lactobacillus (DM8909) growth promoter, obtaining Deshi Lactobacillus (DM8909) ecological mother solution.
The manufacture method of described Deshi Lactobacillus (DM8909) conditioning liquid, gets ecological mother solution 200ml, adds cation guar gum 1.5g, Mentholum, finally add deionized water to 1000ml, subpackage, inspection, warehouse-in.Obtained ecological vagina cleaning conditioning liquid.
The beneficial effect of patent of the present invention: this product raw materials is nontoxic, basic three-waste free discharge, environment is had no adverse effects substantially, its biotechnology, with high content of technology, production technology simply, is easily accomplished scale production, promote the upgrading of the disposable care product of women, there is the harmful bacteria killed in vagina, the Growth and reproduction of protection and promotion probiotics, recover vagina normal acidic environment, maintain colony balance in vagina and can eliminate the infection of all kinds of gynaecopathia simultaneously fast; Progressively can realize the infiltration and development of enterprise to Bio-pharmaceutical Industry, the future development that enterprise product can be made to be advised to multi-product, many product by single product, promotion industry restructuring, increase the weight of popularization and the construction of independent brand; Not only long-term continuing property benefit of holding can be brought to increase but also benefit and the development that can drive drug distributor, Retailed pharmacy for enterprise, expand social benefit, also the problems such as body and mind is unhealthy, life is worried are brought because suffering from gynaecopathia, for numerous women bring out and out Gospel for solving numerous women; Have positive promotion meaning to regional economy and industry restructuring, to promotion personnel employment, social stability has positive effect.
Accompanying drawing explanation
Further accompanying drawing explanation will be carried out below to this patent;
Fig. 1, the process chart that cultivation breeding and conditioning liquid thereof for this patent Deshi Lactobacillus (DM8909) make.
Detailed description of the invention
Shown in Fig. 1, the manufacture method of the clean cloudy conditioning liquid of a kind of women, is characterized in that the cultivation breeding of Deshi Lactobacillus (DM8909) and the manufacture method of conditioning liquid thereof.
Described Deshi Lactobacillus (DM8909) cultivation breeding, by probiotics Deshi Lactobacillus (DM8909) bacterial strain seed inclined plane inoculating after seed bottle 37 DEG C cultivate 48 hours; Be inoculated in shake-flask seed culture medium, cultivate 48 hours for 37 DEG C; Shake-flask seed is being inoculated in fermentor cultivation 24 hours, centrifugalize, is obtaining fermented supernatant fluid, adding Deshi Lactobacillus (DM8909) growth promoter, obtaining Deshi Lactobacillus (DM8909) ecological mother solution.
The manufacture method of described Deshi Lactobacillus (DM8909) conditioning liquid, get ecological mother solution 200ml, add sodium carboxymethyl cellulose (CMC) 1.5g, adding and being dissolved in the 2ml Oleum menthae in 10ml ethanol in advance, finally add deionized water and obtain ecological vagina cleaning conditioning liquid to 1000ml.
Be below the inspection of this patent and experimental technique and result thereof:
Example one
Germicidal test and stability test: 1, test strain: escherichia coli (8099), staphylococcus aureus (ATCC 6538), Candida albicans (ATCC 10231); 2, nertralizer Selection experiment: test organisms is staphylococcus aureus (ATCC 25923), Candida albicans (ATCC 10231).Test establishes parallel 8 groups.Undertaken [1] by quantitative bacteric idal experiment method.1. disinfectant+bacteria suspension; 2. (disinfectant+bacteria suspension)+neutralizer; 3. nertralizer+bacteria suspension; 4. (disinfectant+neutralizer)+bacteria suspension; 5. normal bacteria contrast; 6. do not add bacterium PBS to contrast; 7. the contrast of bacterium nertralizer is not added; 8. the culture medium contrast of strain is not connect., result of the test, with 6,7,8 groups not long bacterium, differs < 10% between 3,4,5 groups of groups, the 1st group of not long bacterium, the 2nd group leader bacterium number more than 100cfu/ml, for nertralizer used and concentration thereof are suitable for; 3, bactericidal assay: washed down by test organisms 24h slant culture PBS, makes bacteria suspension (bacterium liquid melting concn is: bacterial count recovered is 1 × 104 CFU/mL) ~ 9 × 104CFU/ mL).
Preparation 5mL disinfectant solution sample is in 12 × 75 test tubes, get above-mentioned bacteria suspension 100 μ l, add in sample and mix timing, effect is to 2min, drawing 0.5mL is added on containing 4.5mL nertralizer in vitro, abundant mixing, do suitably dilution, draw 0.5mL respectively, be placed in two plates, with the cool nutrient agar (staphylococcus aureus to about 45 DEG C, escherichia coli) or sabouraud's agar (Candida albicans) 15mL pour into, rotate plate, make it full and uniform, after agar solidification, upset is dull and stereotyped, cultivate 48h(staphylococcus aureus for 35 DEG C ± 2 DEG C, escherichia coli) or 72h(Candida albicans), do viable bacteria colony counting, do positive control to regain simultaneously.
Table 1 conditioning liquid nertralizer authentication test results
Table 2 conditioning liquid is to the bactericidal action of staphylococcus aureus
Table 3 conditioning liquid is to colibacillary bactericidal action
Table 4 conditioning liquid is to the bactericidal action of Candida albicans
Stability test: test method: accelerated test: to be put by original packing sample in 37 DEG C ~ 40 DEG C calorstats 3 months, keeps relative humidity >=75 %, and bactericidal property test is carried out in sampling.
Table 5 is incubated the bactericidal action of rear conditioning liquid to Candida albicans
Example two
An animal vaginal mucosa irritation experiment: 1. experiment material: 1. experimental rabbit 2. disposable syringe, cotton swab, catheters etc. are Deshi Lactobacillus (DM8909) microbial ecological agent 3.; 2. experimental technique: 1. experiment point tested material group and negative control group; Often organize 3, gently insert vagina 4cm by after the moistening of conduit washing liquid, and slowly inject 2ml washing liquid with syringe.Matched group sterile saline does same process; 24h after contamination, adopt aeroembolism method to put to death animal, cut open the belly and take out complete vagina, longitudinally cut, whether perusal has the phenomenon such as hyperemia, edema, 2. according to " standards of grading of vaginal mucosa irritation reaction " perusal, histological examination, pathological section.Experimentally data analysis writes out Deshi Lactobacillus (DM8909) microbial ecological agent animal vaginal mucosa irritation laboratory report.
Table 6 conditioning liquid is to vaginal mucosa irritation reaction scoring
Example three
Animal intact skin stimulation test: laboratory animal: each test at least needs the healthy rabbits that 4 skin is intact.Operation sequence: an intact skin irritant test: (1) 24h before the test, cuts the hair of family's rabbit back spinal column both sides, not injured skin.Unhairing scope, left and right each about 3cm × 3cm.Get washing liquid 0.5ml directly to drip on the side unhairing intact skin that area is 2.5cm × 2.5cm, then gauze is applied ointment or plaster, and covers, then use non-stimulated immobilization with adhesive tape with the non-stimulated plastic foil of one deck.Opposite side skin of unhairing is as blank.Apply ointment or plaster 4h, removes residue with warm water.To remove after washing liquid 1,24,48h observes and smears position dermoreaction.Time point the highest skin irritation index judgement skin irritation intensity is observed to receive examination animal.
Table 7 conditioning liquid is to experimental animal intact skin stimulation test result
In sum, through above-mentioned experimental result, show that this patent is applied to biotechnology research, exploitation safety, efficient ecological are used for the clean cloudy care product of Tiny ecosystem of the clean the moon of women, be not only numerous women releasing because suffering from the misery of gynaecopathia, also women is in health, also solves the healthy Lifestyle of numerous women.
Claims (3)
1. a manufacture method for the clean cloudy conditioning liquid of women, is characterized in that the cultivation breeding of Deshi Lactobacillus (DM8909) and the manufacture method of conditioning liquid thereof.
2. the manufacture method of the clean cloudy conditioning liquid of a kind of women according to claim 1, it is characterized in that the breeding of described Deshi Lactobacillus (DM8909), by probiotics Deshi Lactobacillus (DM8909) bacterial strain seed inclined plane inoculating after seed bottle 37 DEG C cultivate 48 hours; Be inoculated in shake-flask seed culture medium, cultivate 48 hours for 37 DEG C; Shake-flask seed is being inoculated in fermentor cultivation 24 hours, centrifugalize, is obtaining fermented supernatant fluid, adding Deshi Lactobacillus (DM8909) growth promoter, obtaining Deshi Lactobacillus (DM8909) ecological mother solution.
3. the manufacture method of the clean cloudy conditioning liquid of a kind of women according to claim 1, it is characterized in that the manufacture method of described Deshi Lactobacillus (DM8909) conditioning liquid, get ecological mother solution 200ml, add cation guar gum 1.5g, Mentholum, finally adds deionized water and obtains ecological vagina cleaning conditioning liquid to 1000ml.
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| CN201310601058.7A CN104644533A (en) | 2013-11-25 | 2013-11-25 | Preparation method of feminine care lotion |
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| CN201310601058.7A CN104644533A (en) | 2013-11-25 | 2013-11-25 | Preparation method of feminine care lotion |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101543514A (en) * | 2009-05-06 | 2009-09-30 | 大连润生堂生物科技工程有限公司 | Compound lactobacillus vagina cleaning hygiene body lotion for preventing or treating vaginitis and technology thereof |
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2013
- 2013-11-25 CN CN201310601058.7A patent/CN104644533A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101543514A (en) * | 2009-05-06 | 2009-09-30 | 大连润生堂生物科技工程有限公司 | Compound lactobacillus vagina cleaning hygiene body lotion for preventing or treating vaginitis and technology thereof |
Non-Patent Citations (2)
| Title |
|---|
| 李佳凤: "《治疗细菌性阴道炎的药物——德氏乳杆菌DM8909活菌制剂》", 《海峡药学》 * |
| 袁杰利等: "《对德氏乳杆菌DM8909菌株的发酵工艺研究》", 《中国微生态学杂志》 * |
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Application publication date: 20150527 |