CN104642094A - Improvement method for culturing stems by using sulfurous acid - Google Patents

Improvement method for culturing stems by using sulfurous acid Download PDF

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Publication number
CN104642094A
CN104642094A CN201510054559.7A CN201510054559A CN104642094A CN 104642094 A CN104642094 A CN 104642094A CN 201510054559 A CN201510054559 A CN 201510054559A CN 104642094 A CN104642094 A CN 104642094A
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CN
China
Prior art keywords
stem
cut
sugarcane
solution
sulfurous acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201510054559.7A
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Chinese (zh)
Inventor
李翔
王伦旺
雷敬超
黄海荣
邓宇驰
唐仕云
黄曲燕
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Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences
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Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences
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Application filed by Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences filed Critical Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences
Priority to CN201510054559.7A priority Critical patent/CN104642094A/en
Publication of CN104642094A publication Critical patent/CN104642094A/en
Pending legal-status Critical Current

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Abstract

The invention discloses an improvement method for culturing stems by using sulfurous acid. The improvement method comprises the main steps of selecting and treating sugarcane flower heads; preparing a sulfurous-acid solution; manufacturing a stem-culturing container; clamping the container under the sugarcane stems, filling the sulfurous-acid solution; replacing the solution at certain time and cutting the stems; and controlling the temperature and the humidity in a greenhouse. The improvement method disclosed by the invention has the advantages that the formula and the steps of the stem-culturing method with the sulfurous-acid solution are improved, so that the fresh-keeping effect of the sugarcane stems is better than that of the prior art, the hybridizing efficiency is improved, the cost is reduced and the operation is convenient.

Description

A kind of sulfurous acid supports the improvement method of stem
Technical field
The present invention relates to agricultural technology field, specifically, relate to the improvement method that a kind of sulfurous acid supports stem.
Background technology
Sugarcane sulfurous acid is supported stem method and is one and can ensures the practical technique that sugarcane sugarcane stem carries out metabolism within a certain period of time and completes to blossom and bear fruit, it mainly utilizes the preservative effect of sulfurous acid solution to keep sugarcane sugarcane stem to start to the freshness in results sub-processes in hybridization, especially to the Parent missing phimosis time or the not long root of phimosis, adopt sulfurous method can also improve blossoming and bearing fruit of colored fringe, receive good effect.Although at present to support stem method used by part sugarcane crossbreeding department for sulfurous acid, have that method imperfection, process are loaded down with trivial details, high in cost of production shortcoming.
Summary of the invention
The object of the invention is to the defect overcoming the existence of above-mentioned technology, there is provided a kind of sulfurous acid to support the improvement method of stem, the method improves formula and the step that sulfurous acid supports stem method, makes sugarcane stem fresh-keeping effect be better than original technology, improve hybridization efficiency, to reduce costs and easy to operate.
Its concrete technical scheme is:
Sulfurous acid supports an improvement method for stem, comprises the following steps:
The selection of step 1. sugarcane fringe and process
Hybridize season sugarcane, namely cut off whole blade after being broken apart by chopping by the colored fringe sugarcane stem of blooming, take back the length that Hybridization Greenhouse intercepts below boot leaf 2 ~ 3m again, cut and be placed on greenhouse water tank;
The configuration of step 2. sulfurous acid solution
Measure sulfurous acid solution 30 ~ 35mL with graduated cylinder to pour in the water of 30 jin and carry out agitation and dilution;
Step 3. supports the preparation of stem container
At the bottom of utilizing the beverage bottle of 1.25L to cut off bottle or the part of 10cm below bottle cap, mix clip with rope preparation through this container;
Container is sandwiched in the below of sugarcane stem by step 4., fills sulfurous acid solution;
Step 5. solution replacing construction and cut stem
Cut stem method: utilize shrub and hedge trimmer up to be cut off at 2cm place by sugarcane stem undercut;
Bloom before terminating, change a solution and cut stem every 2-3 days, bloom after terminating, flower of female parent fringe sugarcane stem was changed a solution every 4-5 days and is cut stem, and through after-ripening in 25-35 days, the moisture of recycling oven for drying seed, finally obtained hybrid seed;
Temperature and Humidity Control in step 6. greenhouse
Utilize Temperature and Humidity Control equipment that temperature should be remained on 26-28 DEG C, humid control is at 60-80%.
Preferably, step 2 sulfite solution is sulphur dioxide 6% aqueous solution, during use within half a year date of manufacture.
Compared with prior art, beneficial effect of the present invention is:
1. namely the sulfurous acid solution in the inventive method joins i.e. use, simple to operate, ensure that the freshness of solution.
2. the method for the invention is more cost-saving than tuns such as original barreleds, changes solution more convenient.
3. stem and Temperature and Humidity Control technology are cut in technical solution of the present invention cooperation, can improve foster stem effect and effectively spend fringe rate.
4. in specific implementation process of the present invention, the Sugarcane Breeding that some misses phimosis time or the not long root of phimosis can be made full use of, improve hybridization efficiency.
Embodiment
Below in conjunction with specific embodiment, technical scheme of the present invention is described in more detail.
Embodiment 1
Sulfurous acid supports an improvement method for stem, comprises the following steps:
The selection of step 1. sugarcane fringe and process
Hybridize season sugarcane, namely cut off whole blade after being broken apart by chopping by the colored fringe sugarcane stem of blooming, take back the length that Hybridization Greenhouse intercepts below boot leaf 2 ~ 3m again, cut and be placed on greenhouse water tank;
The configuration of step 2. sulfurous acid solution
(being equivalent to concentration is 120 × 10 to measure sulfurous acid solution (sulphur dioxide 6% aqueous solution, can not exceed half a year date of manufacture during use) 30mL with graduated cylinder -6) pour in the water of 30 jin and carry out agitation and dilution;
Step 3. supports the preparation of stem container
At the bottom of utilizing the beverage bottle of 1.25L to cut off bottle or the part of 10cm below bottle cap, mix clip with rope preparation through this container;
Container is sandwiched in the below of sugarcane stem by step 4., fills sulfurous acid solution;
Step 5. solution replacing construction and cut stem
Better supporting stem effect to reach, not only needing to change fresh sulfurous acid solution at reasonable time, more need to cut stem to sugarcane stem while replacing solution.
Cut stem method: utilize shrub and hedge trimmer up to be cut off at 2cm place by sugarcane stem undercut;
Bloom before terminating, changed a solution every 2 days and cut stem, bloom after terminating, flower of female parent fringe sugarcane stem was changed a solution every 4 days and cuts stem, and through after-ripening in 25 days, the moisture of recycling oven for drying seed, finally obtained hybrid seed;
Temperature and Humidity Control in step 6. greenhouse
In crossover process medium temperature chamber, the control of temperature and humidity is also very crucial, if humidity is low, temperature is high, and the colored fringe of supporting stem is easy to exhaustion.Inexhausted in order to ensure flower fringe, hybridization obtains seed, and can utilize Temperature and Humidity Control equipment that temperature should be remained on 26 DEG C, humid control is 60%.
Embodiment 2
Sulfurous acid supports an improvement method for stem, comprises the following steps:
The selection of step 1. sugarcane fringe and process
Hybridize season sugarcane, namely cut off whole blade after being broken apart by chopping by the colored fringe sugarcane stem of blooming, take back the length that Hybridization Greenhouse intercepts below boot leaf 2 ~ 3m again, cut and be placed on greenhouse water tank;
The configuration of step 2. sulfurous acid solution
(being equivalent to concentration is 140 × 10 to measure sulfurous acid solution (sulphur dioxide 6% aqueous solution, can not exceed half a year date of manufacture during use) 35mL with graduated cylinder -6) pour in the water of 30 jin and carry out agitation and dilution;
Step 3. supports the preparation of stem container
At the bottom of utilizing the beverage bottle of 1.25L to cut off bottle or the part of 10cm below bottle cap, mix clip with rope preparation through this container;
Container is sandwiched in the below of sugarcane stem by step 4., fills sulfurous acid solution;
Step 5. solution replacing construction and cut stem
Better supporting stem effect to reach, not only needing to change fresh sulfurous acid solution at reasonable time, more need to cut stem to sugarcane stem while replacing solution.
Cut stem method: utilize shrub and hedge trimmer up to be cut off at 2cm place by sugarcane stem undercut;
Bloom before terminating, changed a solution every 3 days and cut stem, bloom after terminating, flower of female parent fringe sugarcane stem was changed a solution every 5 days and cuts stem, and through after-ripening in 35 days, the moisture of recycling oven for drying seed, finally obtained hybrid seed;
Temperature and Humidity Control in step 6. greenhouse
In crossover process medium temperature chamber, the control of temperature and humidity is also very crucial, if humidity is low, temperature is high, and the colored fringe of supporting stem is easy to exhaustion.Inexhausted in order to ensure flower fringe, hybridization obtains seed, and can utilize Temperature and Humidity Control equipment that temperature should be remained on 28 DEG C, humid control is 80%.
Embodiment 3
Sulfurous acid supports an improvement method for stem, comprises the following steps:
The selection of step 1. sugarcane fringe and process
Hybridize season sugarcane, namely cut off whole blade after being broken apart by chopping by the colored fringe sugarcane stem of blooming, take back the length that Hybridization Greenhouse intercepts below boot leaf 2 ~ 3m again, cut and be placed on greenhouse water tank;
The configuration of step 2. sulfurous acid solution
(being equivalent to concentration is 120 × 10 to measure sulfurous acid solution (sulphur dioxide 6% aqueous solution, can not exceed half a year date of manufacture during use) 30mL with graduated cylinder -6) pour in the water of 30 jin and carry out agitation and dilution;
Step 3. supports the preparation of stem container
At the bottom of utilizing the beverage bottle of 1.25L to cut off bottle or the part of 10cm below bottle cap, mix clip with rope preparation through this container;
Container is sandwiched in the below of sugarcane stem by step 4., fills sulfurous acid solution;
Step 5. solution replacing construction and cut stem
Better supporting stem effect to reach, not only needing to change fresh sulfurous acid solution at reasonable time, more need to cut stem to sugarcane stem while replacing solution.
Cut stem method: utilize shrub and hedge trimmer up to be cut off at 2cm place by sugarcane stem undercut;
Bloom before terminating, changed a solution every 3 days and cut stem, bloom after terminating, flower of female parent fringe sugarcane stem was changed a solution every 5 days and cuts stem, and through after-ripening in 32 days, the moisture of recycling oven for drying seed, finally obtained hybrid seed;
Temperature and Humidity Control in step 6. greenhouse
In crossover process medium temperature chamber, the control of temperature and humidity is also very crucial, if humidity is low, temperature is high, and the colored fringe of supporting stem is easy to exhaustion.Inexhausted in order to ensure flower fringe, hybridization obtains seed, and can utilize Temperature and Humidity Control equipment that temperature should be remained on 267 DEG C, humid control is 70%.
The above; be only the present invention's preferably embodiment; protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses, the simple change of the technical scheme that can obtain apparently or equivalence are replaced and are all fallen within the scope of protection of the present invention.

Claims (2)

1. sulfurous acid supports an improvement method for stem, it is characterized in that, comprises the following steps:
The selection of step 1. sugarcane fringe and process
Hybridize season sugarcane, namely cut off whole blade after being broken apart by chopping by the colored fringe sugarcane stem of blooming, take back the length that Hybridization Greenhouse intercepts below boot leaf 2 ~ 3m again, cut and be placed on greenhouse water tank;
The configuration of step 2. sulfurous acid solution
Measure sulfurous acid solution 30 ~ 35mL with graduated cylinder to pour in the water of 30 jin and carry out agitation and dilution;
Step 3. supports the preparation of stem container
At the bottom of utilizing the beverage bottle of 1.25L to cut off bottle or the part of 10cm below bottle cap, mix clip with rope preparation through this container;
Container is sandwiched in the below of sugarcane stem by step 4., fills sulfurous acid solution;
Step 5. solution replacing construction and cut stem
Cut stem method: utilize shrub and hedge trimmer up to be cut off at 2cm place by sugarcane stem undercut;
Bloom before terminating, change a solution and cut stem every 2-3 days, bloom after terminating, flower of female parent fringe sugarcane stem was changed a solution every 4-5 days and is cut stem, and through after-ripening in 25-35 days, the moisture of recycling oven for drying seed, finally obtained hybrid seed;
Temperature and Humidity Control in step 6. greenhouse
Utilize Temperature and Humidity Control equipment that temperature should be remained on 26-28 DEG C, humid control is at 60-80%.
2. sulfurous acid according to claim 1 supports the improvement method of stem, it is characterized in that, step 2 sulfite solution is sulphur dioxide 6% aqueous solution, during use within half a year date of manufacture.
CN201510054559.7A 2015-02-02 2015-02-02 Improvement method for culturing stems by using sulfurous acid Pending CN104642094A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110741922A (en) * 2019-10-25 2020-02-04 广西壮族自治区农业科学院 Preparation method of rooting-free stem-nourishing solution for sugarcane hybrid seed production

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101401543A (en) * 2008-11-04 2009-04-08 广州甘蔗糖业研究所 Sugarcane cut stem hybridization method for production of hybrid seeds at tropical monsoon climate area
CN102792884A (en) * 2012-08-21 2012-11-28 广州甘蔗糖业研究所 Indoor sugarcane hybridization pollination method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101401543A (en) * 2008-11-04 2009-04-08 广州甘蔗糖业研究所 Sugarcane cut stem hybridization method for production of hybrid seeds at tropical monsoon climate area
CN102792884A (en) * 2012-08-21 2012-11-28 广州甘蔗糖业研究所 Indoor sugarcane hybridization pollination method

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
戚经文等: "《甘蔗育种和良种繁育》", 31 December 1980, 农业出版社 *
王丽萍: ""浅析内陆高原甘蔗有性杂交技术"", 《云南农业科技》 *
王丽萍: ""甘蔗亚硫酸养茎杂交初报"", 《云南农业科技》 *
陈西文: ""应用甘蔗亚硫酸法杂交的几个问题"", 《甘蔗糖业》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110741922A (en) * 2019-10-25 2020-02-04 广西壮族自治区农业科学院 Preparation method of rooting-free stem-nourishing solution for sugarcane hybrid seed production

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