CN104630904A - Bacteria and high-temperature alkali degumming combined method for preparing cotton stalk husk fibers - Google Patents
Bacteria and high-temperature alkali degumming combined method for preparing cotton stalk husk fibers Download PDFInfo
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- CN104630904A CN104630904A CN201510035290.8A CN201510035290A CN104630904A CN 104630904 A CN104630904 A CN 104630904A CN 201510035290 A CN201510035290 A CN 201510035290A CN 104630904 A CN104630904 A CN 104630904A
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- Prior art keywords
- cotton stalk
- pectin
- bacterium
- temperature
- mineral salt
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01C—CHEMICAL OR BIOLOGICAL TREATMENT OF NATURAL FILAMENTARY OR FIBROUS MATERIAL TO OBTAIN FILAMENTS OR FIBRES FOR SPINNING; CARBONISING RAGS TO RECOVER ANIMAL FIBRES
- D01C1/00—Treatment of vegetable material
Abstract
The invention relates to a method for preparing cotton stalk husk fibers by combining bacteria and high-temperature alkali degumming. The preparation method comprises the following steps: step 1. collecting and screening strains; sterilely collecting sediment from a cotton stalk retting pond; after enriching the sediment in a culture solution; collecting liquid supernatant and inoculating the liquid supernatant on a pectin-agar flat plate; screening high-pectin decomposition enzyme activity bacterial strains; step 2. degumming by the bacteria; pre-treating cotton stalk husks with a mineral salt solution; inoculating the screened bacterial strains and vibrating at 36-40 DEG C for 18-22 days; and step 3. carrying out high-temperature alkali treatment, and treating with a NaOH solution with the mass percent of 3%-5% at 150-170 DEG C for 60-180 minutes. According to the method, thin-wall cells at the peripheries of phloem bundles can be removed by using bacteria degumming, and the lignin removing rate is improved by the high-temperature alkali treatment. The content of the lignin in the cotton stalk husk fibers is reduced to be lower than 3%. Compared with other methods, the method has the advantages that the modulus of the cotton stalk husk fibers extracted by the method is lower and the cotton stalk husk fibers are more suitable for spinning.
Description
Technical field
The present invention relates to a kind of bacterium, high-temperature alkali comes unstuck the method combining and prepare cotton stalk hide fiber, belongs to textile fabric preparation field.
Background technology
Cotton is as the important industrial crops of China, and annual production reaches more than 500 ten thousand hectares, and the cotton stalk of 3375-3750kg can be produced in per hectare cotton field, and cotton stalk tare weight amount accounts for about 25% of cotton stalk weight.Be rich in cellulose, lignin and pentosan etc. in cotton stalk skin, there is multiple value.Therefore, from cotton stalk skin, extract the cotton stalk hide fiber being suitable for weaving, prepared by composite, contribute to the value improving cotton crop.But in a long time, cotton stalk major part is taken as waste material and burns or stack and rot in ground, field, and utilization rate is low, causes the significant wastage of resource.
At present, the patent preparing cotton stalk hide fiber mainly contains: (1) adopts the quick-fried equipment of sudden strain of a muscle to carry out preliminary treatment to cotton stalk skin, as patent of invention " a kind of cotton stalk hide fiber cleans Degumming method " (publication number: CN101008108A), the quick-fried method combined with High Temperature High Pressure post processing of sudden strain of a muscle is adopted to prepare cotton stalk hide fiber; (2) biological enzyme prepares cotton stalk hide fiber, as patent of invention " biotechnology prepares the method for cotton stalk bast textile industry fiber " (publication number: CN102660778A) processes cotton stalk skin with compositional biological enzyme; (3) chemical method prepares cotton stalk hide fiber, as patent of invention " a kind of cotton stalk directly comes unstuck and prepares the method for cotton stalk hide fiber cellulose fiber " (publication number: CN102286789A) describes and prepares cotton stalk hide fiber by the method for normal temperature alkali treatment; Hemicellulose when patent of invention " the low alkali of a kind of high temperature cotton stalk hide fiber quick degumming method " (publication number: CN101509151A) adopts High Temperature High Pressure low alkali technology in cotton stalk skin and lignin depth degradation; The method that patent of invention " keen-witted and capable fiber of cotton stalk skin and preparation method thereof " (publication number: CN103255484A) adopts pickling and High Temperature High Pressure boiling-off to combine prepares cotton stalk hide fiber.Patent of the present invention adopts bacterium, the legal cotton stalk hide fiber preparing low modulus of high-temperature alkali decementation.
Summary of the invention
The object of this invention is to provide a kind of method of bacterium, the cotton stalk hide fiber of the legal preparation of high-temperature alkali decementation, the obtained cotton stalk hide fiber that fineness is thin, modulus is low.And the present invention first gathers and screens artificial bio-membrane bacterium, bacterium is carried out to cotton stalk skin and comes unstuck.
The technical solution adopted in the present invention is that a kind of bacterium, high-temperature alkali being come unstuck combines the method for the cotton stalk hide fiber of preparation, specifically implements according to following steps:
(1) bacterial classification collection and screening: aseptic collection deposit from the pond of retting cotton stalk, enrichment 8-12 time in nutrient solution.Nutrient solution consists of: K
2hPO
40.5-2.5g/L, MgSO
40.5-2.5g/L, NaNO
31-5g/L, FeSO
40.01-0.05g/L, pectin 1-5g/L, and by the aseptic NaOH solution adjust ph of 1mol/L to 7-9.Be seeded in after serial dilution by supernatant liquor on pectin-agar plate, pectin-agar consists of: K
2hPO
40.5-2.5g/L, MgSO
40.5-2.5g/L, NaNO
31-5g/L, FeSO
40.01-0.05g/L, pectin 1-5g/L, agar 10-30g/L, and in advance pH value is adjusted to 7-9.Cultivate 60-80h at 28-32 DEG C after, be coated on pectin-agar plate with the congo red solution that mass percent concentration is 0.01-0.05%.After placing 3-5h under room temperature, gnotobasis, bacterial strain the coming unstuck for next step of screening larger diameter magenta color hydrolysis circle.
(2) bacterium is come unstuck: first use the cotton stalk skin of mineral salt solution preliminary treatment, mineral salt solution pH value is 6-8, treatment temperature 80-100 DEG C, processing time 10-30min.Mineral salt solution forms: K
2hPO
40.5-2.5g, NaNO
31-5g.Enter in conical flask by pretreated for mineral salt solution cotton stalk leather jacket, inoculate the bacterial strain screened in a ring step (1), vibrate 18-22 days at 36-40 DEG C, rotating speed 160-200rpm.Cotton stalk skin tap water after being come unstuck by bacterium also at room temperature dries in the shade.
(3) high temperature alkali cooking comes unstuck: the cotton stalk skin after coming unstuck to bacterium in step (2), and employing mass percent concentration is the NaOH solution process of 3-5%, treatment temperature 150-170 DEG C, solid-to-liquid ratio 1: 20-1: 40, time 60-180min.Cotton stalk hide fiber after alkali treatment immediately first with sig water washing, then uses clear water rinsing to neutral, is the stainless steel comb combing of 10 ~ 15 teeth/cm after at room temperature drying in the shade by pin tooth density.
In above each operation:
(1) enrichment of bacterial classification have employed with pectin is the synthesis nutrient solution of sole carbon source, avoids filtering out with other materials the bacterial classification being carbon source.
(2) on pectin agar plate, be coated with Congo red solution, its effect filters out the bacterial strain having high fructose catabolic enzyme and live.
(3) by mineral salt solution preliminary treatment main purpose be the bacterium of going out except cotton stalk skin carries.
(4) bacterium is come unstuck is to be separated with phloem bundle by the parenchyma cell in cotton stalk bark, creates conditions, shorten fiber extraction time for alkali enters phloem bundle inside fast and fully reacts with the lignin in phloem bundle.
(5) high-temperature alkali process improves the speed that removes and the efficiency of lignin.
(6) be first that the lignin that prevents from having removed and hemicellulose again again condense upon fiber surface in cleaning process with the effect that alkali lye is washed after high-temperature alkali process.
(7) to come unstuck and fiber after high-temperature alkali process adopts under room temperature condition and dries in the shade, and do not use the mode of oven dry, mainly prevent lignin to be polymerized adhesion under alkali-free condition, make the easy combing of fiber softening.
Detailed description of the invention
Below in conjunction with specific embodiment, the invention will be further described.
The present invention first cultivates artificial bio-membrane bacterium, filters out the bacterial strain having high fructose catabolic enzyme and live, then carries out bacterium to cotton stalk skin and come unstuck, last high-temperature alkali process further, the cotton stalk hide fiber of obtained low modulus.
A kind of bacterium, the cotton stalk hide fiber of the legal preparation of high-temperature alkali decementation
Embodiment 1:
(1) bacterial classification collection and screening: aseptic collection deposit from the pond of retting cotton stalk, enrichment 8 times in nutrient solution.Nutrient solution consists of: K
2hPO
40.5g/L, MgSO
41g/L, NaNO
32g/L, FeSO
40.02g/L, pectin 3g/L, and by 1mol/L aseptic NaOH solution adjust ph to 7.5.Be seeded in after serial dilution by supernatant liquor on pectin-agar plate, pectin-agar consists of: containing K
2hPO
40.5g/L, MgSO
41g/L, NaNO
32g/L, FeSO
40.02g/L, pectin 3g/L, agar 25g/L, and in advance pH value is adjusted to 7.5.Cultivate 75h at 31 DEG C after, be that the congo red solution of 0.03% is coated on pectin-agar plate by mass percent concentration.After placing 3.5h under room temperature, gnotobasis, bacterial strain the coming unstuck for next step of screening larger diameter magenta color hydrolysis circle.
(2) bacterium is come unstuck: first use the cotton stalk skin of mineral salt solution preliminary treatment, mineral salt solution pH value is 7.5, treatment temperature 95 DEG C, processing time 25min.Mineral salt solution forms: K
2hPO
40.5g, NaNO
32g.Pretreated for mineral salt solution cotton stalk leather jacket is entered in conical flask, inoculates the bacterial strain screened in a ring step (1), vibrate 18 days at 40 DEG C, rotating speed 200rpm.Cotton stalk skin tap water after being come unstuck by bacterium also at room temperature dries in the shade.
(3) high-temperature alkali process: the cotton stalk skin after coming unstuck to bacterium in step (2), employing mass percent concentration is the NaOH solution process of 3.5%, temperature 150 DEG C, solid-to-liquid ratio 1: 20, time 180min.Cotton stalk hide fiber after alkali treatment immediately first with sig water washing, then uses clear water rinsing to neutral, is the stainless steel comb combing of 10 ~ 15 teeth/cm after at room temperature drying in the shade by pin tooth density.
Embodiment 2:
(1) bacterial classification collection and screening: aseptic collection deposit from the pond of retting cotton stalk, enrichment 10 times in nutrient solution.Nutrient solution consists of: K
2hPO
41g/L, MgSO
40.5g/L, NaNO
33g/L, FeSO
40.01g/L, pectin 2g/L, and by 1mol/L aseptic NaOH solution adjust ph to 7.Be seeded in after serial dilution by supernatant liquor on pectin-agar plate, pectin-agar consists of: K
2hPO
41g/L, MgSO
40.5g/L, NaNO
33g/L, FeSO
40.01g/L, pectin 2g/L, agar 20g/L, and in advance pH value is adjusted to 7.0.Cultivate 72h at 30 DEG C after, be coated on pectin-agar plate with the congo red solution that mass percent concentration is 0.02%.After placing 4h under room temperature, gnotobasis, bacterial strain the coming unstuck for next step of screening larger diameter magenta color hydrolysis circle.
(2) bacterium is come unstuck: first use the cotton stalk skin of mineral salt solution preliminary treatment, mineral salt solution pH value is 7, treatment temperature 100 DEG C, processing time 20min.Mineral salt solution forms: K
2hPO
41g, NaNO
33g.Pretreated for mineral salt solution cotton stalk leather jacket is entered in conical flask, inoculates the bacterial strain screened in a ring step (1), vibrate 20 days at 37 DEG C, rotating speed 180rpm.Cotton stalk skin tap water after being come unstuck by bacterium also at room temperature dries in the shade.
(3) high-temperature alkali process: the cotton stalk skin after coming unstuck to bacterium in step (2), employing mass percent concentration is the NaOH solution process of 4%, temperature 160 DEG C, solid-to-liquid ratio 1: 30, time 120min.Cotton stalk hide fiber after alkali treatment immediately first with sig water washing, then uses clear water rinsing to neutral, is the stainless steel comb combing of 12.5 teeth/cm after at room temperature drying in the shade by pin tooth density.
Embodiment 3:
(1) bacterial classification collection and screening: aseptic collection deposit from the pond of retting cotton stalk, enrichment 12 times in nutrient solution.Nutrient solution consists of: K
2hPO
41.5g/L, MgSO
41.5g/L, NaNO
34g/L, FeSO
40.03g/L, pectin 4g/L, and by 1mol/L aseptic NaOH solution adjust ph to 8.Be seeded in after serial dilution by supernatant liquor on pectin-agar plate, pectin-agar consists of: K
2hPO
41.5g/L, MgSO
41.5g/L, NaNO
34g/L, FeSO
40.03g/L, pectin 4g/L, agar 30g/L, and in advance pH value is adjusted to 8.0.Cultivate 80h at 32 DEG C after, be coated on pectin-agar plate with the congo red solution that mass percent concentration is 0.04%.After placing 3h under room temperature, gnotobasis, bacterial strain the coming unstuck for next step of screening larger diameter magenta color hydrolysis circle.
(2) bacterium is come unstuck: first use the cotton stalk skin of mineral salt solution preliminary treatment, mineral salt solution pH value is 8, treatment temperature 90 DEG C, processing time 30min.Mineral salt solution forms: K
2hPO
41.5g, NaNO
34g.Pretreated for mineral salt solution cotton stalk leather jacket is entered in conical flask, inoculates the bacterial strain screened in a ring step (1), vibrate 22 days at 36 DEG C, rotating speed 190rpm.Cotton stalk skin tap water after being come unstuck by bacterium also at room temperature dries in the shade.
(3) high-temperature alkali process: the cotton stalk skin after coming unstuck to bacterium in step (2), employing mass percent concentration is the NaOH solution process of 4.5%, temperature 170 DEG C, solid-to-liquid ratio 1: 40, time 60min.Cotton stalk hide fiber after alkali treatment immediately first with sig water washing, then uses clear water rinsing to neutral, is the stainless steel comb combing of 15 teeth/cm after at room temperature drying in the shade by pin tooth density.
Claims (1)
1. a bacterium of preparing low modulus cotton stalk hide fiber, high-temperature alkali decementation are legal, it is characterized in that, the method comprises that artificial bio-membrane bacterium is cultivated, bacterium is come unstuck, high-temperature alkali process three steps, and each process feature is as follows:
(1) bacterial classification collection and screening: aseptic collection deposit from the pond of retting cotton stalk, enrichment 8-12 time in nutrient solution.Nutrient solution consists of: K
2hPO
40.5-2.5g/L, MgSO
40.5-2.5g/L, NaNO
31-5g/L, FeSO
40.01-0.05g/L, pectin 1-5g/L, and by the aseptic NaOH solution adjust ph of 1mol/L to 7-9.Be seeded in after serial dilution by supernatant liquor on pectin-agar plate, pectin-agar consists of: K
2hPO
40.5-2.5g/L, MgSO
40.5-2.5g/L, NaNO
31-5g/L, FeSO
40.01-0.05g/L, pectin 1-5g/L, agar 10-30g/L, and in advance pH value is adjusted to 7-9.Cultivate 60-80h at 28-32 DEG C after, be coated on pectin-agar plate with the congo red solution that mass percent concentration is 0.01-0.05%.After placing 3-5h under room temperature, gnotobasis, bacterial strain the coming unstuck for next step of screening larger diameter magenta color hydrolysis circle.
(2) bacterium is come unstuck: first use the cotton stalk skin of mineral salt solution preliminary treatment, mineral salt solution pH value is 6-8, treatment temperature 80-100 DEG C, processing time 10-30min.Mineral salt solution forms: K
2hPO
40.5-2.5g, NaNO
31-5g.Enter in conical flask by pretreated for mineral salt solution cotton stalk leather jacket, inoculate the bacterial strain screened in a ring step (1), vibrate 18-22 days at 36-40 DEG C, rotating speed 160-200rpm.Cotton stalk skin tap water after being come unstuck by bacterium also at room temperature dries in the shade.
(3) high temperature alkali cooking comes unstuck: the cotton stalk skin after coming unstuck to bacterium in step (2), and employing mass percent concentration is the NaOH solution process of 3-5%, treatment temperature 150-170 DEG C, solid-to-liquid ratio 1: 20-1: 40, time 60-180min.Cotton stalk hide fiber after alkali treatment immediately first with sig water washing, then uses clear water rinsing to neutral, is the stainless steel comb combing of 10 ~ 15 teeth/cm after at room temperature drying in the shade by pin tooth density.
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| CN201510035290.8A CN104630904A (en) | 2015-01-21 | 2015-01-21 | Bacteria and high-temperature alkali degumming combined method for preparing cotton stalk husk fibers |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85104285A (en) * | 1985-05-31 | 1987-02-04 | 湖南师范大学 | The cultural method of bacteria which produce ramie degumming enzyme and application |
| CN1048409A (en) * | 1989-06-28 | 1991-01-09 | 武汉大学 | New process for rapid biodegumming of ramie |
| CN1366043A (en) * | 2001-01-15 | 2002-08-28 | 山东大学 | Process for preparing pectinase for degumming ramie and its application in degumming ramie |
| CN102002467A (en) * | 2010-08-13 | 2011-04-06 | 东华大学 | Bacillus cereus DA3 strain and preparation method and application thereof |
| CN102002468A (en) * | 2010-08-13 | 2011-04-06 | 东华大学 | Fluorescent pseudomonad DA4 strain as well as acquisition method and application thereof |
-
2015
- 2015-01-21 CN CN201510035290.8A patent/CN104630904A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85104285A (en) * | 1985-05-31 | 1987-02-04 | 湖南师范大学 | The cultural method of bacteria which produce ramie degumming enzyme and application |
| CN1048409A (en) * | 1989-06-28 | 1991-01-09 | 武汉大学 | New process for rapid biodegumming of ramie |
| CN1366043A (en) * | 2001-01-15 | 2002-08-28 | 山东大学 | Process for preparing pectinase for degumming ramie and its application in degumming ramie |
| CN102002467A (en) * | 2010-08-13 | 2011-04-06 | 东华大学 | Bacillus cereus DA3 strain and preparation method and application thereof |
| CN102002468A (en) * | 2010-08-13 | 2011-04-06 | 东华大学 | Fluorescent pseudomonad DA4 strain as well as acquisition method and application thereof |
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Application publication date: 20150520 |
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