CN104623602B - A kind of Chinese medicine composition for treating syndrome of deficient cold of spleen and stomach and preparation method thereof - Google Patents
A kind of Chinese medicine composition for treating syndrome of deficient cold of spleen and stomach and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of Chinese medicine composition for treating syndrome of deficient cold of spleen and stomach and preparation method thereof, belong to medicine manufacture technology field.Its technical characteristics is made up of following Chinese medicine material：34 parts of dried orange peel, 23 parts of the banksia rose, 9 12 parts of folium artemisiae argyi, 34 parts of cloves, 34 parts of Chinese cassia tree, 23 parts of rhizoma zingiberis, 12 parts of corydalis tuber, 23 parts of fennel seeds, 12 parts of rhizoma atractylodis, 23 parts of evodia rutaecarpa, 23 parts of Radix Angelicae Sinensis, 12 parts of radix bupleuri, 12 parts of frutus cnidii, 12 parts of the root of straight ladybell, 34 parts of honey-fried licorice root.It is prepared by purifying processing processing, co-grinding processing, disinfecting.Prescription compatibility of the present invention is rigorous, and collocation is reasonable, and preparation method is simple.Experiment proves：The present invention has significant protective effect to acute and chronic gastric mucosa damage, can improve hemorheological property, also with invigorating the spleen and promoting blood circulation and removing blood stasis and obvious analgesic activity.It can be used for the illnesss such as deficiency-cold in spleen and stomach, stagnation of cold in middle-JIAO, abdominal distention, crymodynia caused by qi depression to blood stasis, deficiency of food, loose stool.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition and preparation method thereof, more particularly to a kind of Chinese medicine for treating syndrome of deficient cold of spleen and stomach
Composition and preparation method thereof, belong to medicine manufacture technology field.
Background technology
Treatment and functional medicine are broadly divided into for oral administration, injection and external application from application method and route of administration to distinguish
(containing skin, mucous membrane and cavity) and physical treatment, belong to medicine, medicine equipment, health food and health treatment etc..Only
For treatment (function) property product for gastrointestinal system, the approach administration (medicine and health food) such as oral administration, injection
Exist as toxic side effect is big, dosage is big, the voluntarily drawback such as difficult medication；(medicine) is administered by topical route, though can be with
Avoid " first pass effect " of liver, reduce dosage, reduce toxic side effect, but these it is big be required for by extracting, purifying, point
From etc. PROCESS FOR TREATMENT, this generates energy waste, environmental pollution, medicine source destroy etc. problems；Using medicine equipment, not only
It is with high costs, the disorder of human body electromagnetism is easily produced, or even the adverse consequences such as fire occur.At present, pouch are dissipated used in people
Such as vital herb-belt 505, there is the function of " tonifying speen and tonifying kidney, adjustment qi and blood, clearing and activating the channels and collaterals, Yin Yang balancing " after use, preventing and treating
Extensive clinical verification has been obtained in terms of the disease of digestive system.Vital herb-belt 505, preparation technology is simple, does not pollute ring
The puzzlement in border, Chinese herbal medicine powder (powder) is included into special medicine core and forms administration pond, refilled into being worn on human body SHENQUE acupoint in cloth bag
(being commonly called as navel).Connect the vital organs of the human body, the twelve regular channels in SHENQUE acupoint, play the role of inside and outside hinge, form a connecting link, in addition SHENQUE acupoint portion
Position subcutaneous fat is fewer, and abundant blood vessel and lymphatic vessel is distributed with, is advantageous to medicine and penetrates, and easily absorbs, stores and transports.
Mainly through pulse classic in this cave, medicine follows through reaching answered internal organs lunar the spleen channel of the stomach channel of foot-Yang-ming, hand etc..The clinic of more than 20 years is real
Trample and research shows：Vital herb-belt 505 is easy to use, as worn the clothes, with oneself, wears at any time, for epigastric pain, diarrhoea, woman
Female's irregular menstruation, dysmenorrhoea, with illnesss such as the lower and enuresis there is the effect of good.But existing vital herb-belt 505 is formulated into
It is point too many, control that " disease " is excessively numerous and jumbled, and targeting is not strong, and preparation technology is also more complicated, also add the purchase of user into
This.
The content of the invention
Big the invention aims to solve existing vital herb-belt 505 prescription, Chinese medicinal ingredients are more, and it is wide in range to control disease, pin
Property is not protruded, the problems such as preparation technology is relative complex.Therefore, we change sanction, simplify, optimize former prescription, to develop for the 3rd generation
Product provides a kind of new technical scheme.
To reach above-mentioned purpose, the present invention can be achieved through the following technical solutions：
A kind of Chinese medicine composition for treating syndrome of deficient cold of spleen and stomach, it is the medicament made of the raw material of following parts by weight：
Dried orange peel 3-4 parts, banksia rose 2-3 parts, folium artemisiae argyi 9-12 parts, cloves 3-4 parts, Chinese cassia tree 3-4 parts, rhizoma zingiberis 2-3 parts, corydalis tuber 1-
2 parts, fennel seeds 2-3 parts, rhizoma atractylodis 1-2 parts, evodia rutaecarpa 2-3 parts, Radix Angelicae Sinensis 2-3 parts, radix bupleuri 1-2 parts, frutus cnidii 1-2 parts, the root of straight ladybell 1-2
Part, honey-fried licorice root 3-4 parts.
A kind of Chinese medicine composition for treating syndrome of deficient cold of spleen and stomach, it is the medicament made of the raw material of following parts by weight：
3 parts of dried orange peel, 2 parts of the banksia rose, 11 parts of folium artemisiae argyi, 3 parts of cloves, 3 parts of Chinese cassia tree, 2 parts of rhizoma zingiberis, 1 part of corydalis tuber, 2 parts of fennel seeds,
1 part of rhizoma atractylodis, 2 parts of evodia rutaecarpa, 2 parts of Radix Angelicae Sinensis, 1 part of radix bupleuri, 1 part of frutus cnidii, 1 part of the root of straight ladybell, 3 parts of honey-fried licorice root.
A kind of Chinese medicine composition for treating syndrome of deficient cold of spleen and stomach, it is that Chinese medicine is wrapped in pharmacy made of above-mentioned raw materials
All formulations contained.
A kind of preparation method for the Chinese medicine composition for treating syndrome of deficient cold of spleen and stomach, it is prepared into the method and steps of powder preparation formulation
It is：
First, purification, processing processing：Dried orange peel, the banksia rose, folium artemisiae argyi, cloves, Chinese cassia tree, rhizoma zingiberis, corydalis tuber, fennel seeds, rhizoma atractylodis, Wu
The fruit of medicinal cornel, Radix Angelicae Sinensis, radix bupleuri, frutus cnidii, the root of straight ladybell and honey-fried licorice root, purified, processing processing, it is small that 2 are dried under 50~55 DEG C of low temperature
When, in case medicinal；
2nd, co-grinding is handled：Chinese cassia tree, the taste Chinese medicine material co-grinding of cloves 2 are into 24 mesh coarse powder；The banksia rose, honey-fried licorice root, do
Ginger, folium artemisiae argyi, the taste Chinese medicine material co-grinding of radix bupleuri 5 are into 24 mesh coarse powder；After the thick medicinal powder of both the above is mixed with Radix Angelicae Sinensis, fennel fruit
Perfume, frutus cnidii, evodia rutaecarpa, corydalis tuber, the root of straight ladybell, dried orange peel, the taste co-grinding of rhizoma atractylodis 8 sieving, produce mixing medicine into 80 mesh fine powders
Powder；
3rd, disinfect：Take mixed powder in step (2) by 20 grams of dosimetric systems into medicine core, assembling, plastic packaging,60Co shines
Sterilizing is penetrated, packs, produces.
The present invention is compared with prior art advantageously：Prescription is small, and compatibility is rigorous, reasonable, with strong points, treatment
Effect is obvious, and preparation technology is simple etc..The present invention is containing prominent environmental protection principle, the development based on inheritance characteristic of SHENQUE acupoint external application,
Integrate treatment, health-care effect, give full play to " inner disease outer treat " characteristic, normalized zoopery proves：The present invention to it is anxious,
Chronic gastric mucosa damage has significant protective effect, and can improve hemorheological property, also with invigorating the spleen and it is promoting blood circulation and removing blood stasis and
Obvious analgesic activity.The present invention have " warming spleen and stomach for dispelling cold, promoting qi circulation and relieving pain, invigorating spleen and reinforcing stomach " effect, can be used for deficiency-cold in spleen and stomach,
The illness such as abdominal distention, crymodynia, deficiency of food, loose stool caused by stagnation of cold in middle-JIAO, qi depression to blood stasis.
Embodiment
The present invention is described in further detail below in conjunction with specific embodiment：
Embodiment one：
A kind of Chinese medicine composition for treating syndrome of deficient cold of spleen and stomach, it is the medicine made of the raw material of following weight kilogram quantities
Agent：
Dried orange peel 3Kg, banksia rose 2Kg, folium artemisiae argyi 11Kg, cloves 3Kg, Chinese cassia tree 3Kg, rhizoma zingiberis 2Kg, corydalis tuber 1Kg, fennel seeds 2Kg,
Rhizoma atractylodis 1Kg, evodia rutaecarpa 2Kg, Radix Angelicae Sinensis 2Kg, radix bupleuri 1Kg, frutus cnidii 1Kg, the root of straight ladybell 1Kg, honey-fried licorice root 3Kg.
A kind of Chinese medicine composition for treating syndrome of deficient cold of spleen and stomach, it is that medicament is powder preparation formulation made of above-mentioned raw materials.
A kind of preparation method for the Chinese medicine composition for treating syndrome of deficient cold of spleen and stomach, it is the method and steps for being prepared into powder
It is：
First, dried orange peel, the banksia rose, folium artemisiae argyi, cloves, Chinese cassia tree, rhizoma zingiberis, corydalis tuber, fennel seeds, rhizoma atractylodis, evodia rutaecarpa, Radix Angelicae Sinensis, radix bupleuri,
Frutus cnidii, the root of straight ladybell and honey-fried licorice root, purified, processing processing, dried 2 hours under 50~55 DEG C of low temperature, in case medicinal.
2nd, Chinese cassia tree, the taste Chinese medicine material co-grinding of cloves 2 are into 24 mesh coarse powder；The banksia rose, honey-fried licorice root, rhizoma zingiberis, folium artemisiae argyi, radix bupleuri 5
Taste Chinese medicine material co-grinding is into 24 mesh coarse powder；After the thick medicinal powder of both the above is mixed with Radix Angelicae Sinensis, fennel seeds, frutus cnidii, Wu Zhu
Cornel, corydalis tuber, the root of straight ladybell, dried orange peel, the taste co-grinding of rhizoma atractylodis 8 sieving, produce mixed powder into 80 mesh fine powders.
3rd, disinfect：Take mixed powder in step (2) by 20 grams of dosimetric systems into medicine core, assembling, plastic packaging,60Co shines
Sterilizing is penetrated, packs, produces.
Major function：Warming spleen and stomach for dispelling cold, promoting qi circulation and relieving pain, invigorating spleen and reinforcing stomach.Draw for deficiency-cold in spleen and stomach, stagnation of cold in middle-JIAO, qi depression to blood stasis
The abdominal distention that rises, crymodynia, deficiency of food, loose stool etc..
Specification：20g/ medicine cores.
Usage and dosage：External application SHENQUE acupoint, is close to skin-worn.Every bag (medicine core) can be used continuously/wear 4~5 weeks.
It is a course for the treatment of often to wear March.
Embodiment two：
A kind of Chinese medicine composition for treating syndrome of deficient cold of spleen and stomach, it is the medicine made of the raw material of following weight kilogram quantities
Agent：
Dried orange peel 4Kg, banksia rose 3Kg, folium artemisiae argyi 12Kg, cloves 4Kg, Chinese cassia tree 4Kg, rhizoma zingiberis 3Kg, corydalis tuber 2Kg, fennel seeds 2Kg,
Rhizoma atractylodis 2Kg, evodia rutaecarpa 3Kg, Radix Angelicae Sinensis 3Kg, radix bupleuri 2Kg, frutus cnidii 1Kg, the root of straight ladybell 1Kg, honey-fried licorice root 4Kg.
The method and steps of powder is prepared into embodiment one.
The present invention name of product be《Vital herb-belt 505 (the 3rd generation)》.
Prove that the used time is for the validity and hypotoxicity for the treatment of syndrome of deficient cold of spleen and stomach outside the present invention below by way of zoopery.
1st, Report on Animal
Pharmacodynamics test data
Purpose：Study vital herb-belt 505 (the 3rd generation) product to ethanol induced Acute gastric mucosa damage, to rat acetic acid
Cause chronic gastric mucosa damage and the influence of Mice with Spleen model, the shadow to acute blood-stasis model rat blood rheological characteristic are caused to rheum officinale
Ring, the influence of mice pain and the influence to mouse thermostimulation reaction are caused to glacial acetic acid, inquire into it to acute gastric mucosal lesion, slow
Effect, function of invigorating spleen, function of promoting blood circulation to disperse blood clots and the analgesic activity of property gastric mucosa damage.Method：Acute gastric mucosal lesion uses nothing
Water-ethanol gastric mucosa damage model, with vernier caliper measurement blutpunkte or the length and width of bleeding band.With lesion extent index
And mucosa tissue changes into index, the influence to acute gastric mucosal injury is observed.Chronic gastric mucosa damage is using big
Mouse acetic acid inustion, measure its area and volume, influence of the observation medicine to chronic gastric ulcer model.Function of invigorating spleen experiment uses
Continuous gavage rheum officinale decocting liquid causes Mice with Spleen model for 7 days, Observe and measure experiment mice body weight, body temperature, low temperature swimming time,
Intestinal propulsive rate, thymus gland and spleen organ index；Experimental observation promoting blood circulation and removing blood stasis is to acute blood-stasis model rat blood rheological characteristic
Influence；Analgesic activity uses mouse writhing method and hot plate method.As a result：By reagent 3.33g/kg, 1.665g/kg dosage significantly mitigates
Absolute ethyl alcohol causes the lesion extent index of acute gastric mucosal injury and can significantly improve damage inhibiting rate, by reagent 3.33g/
Kg, 1.665g/kg, 0.833g/kg dosage group significantly mitigate the lesion total mark that absolute ethyl alcohol causes acute gastric mucosal injury
Index simultaneously can obviously reduce the rate of injury, prompt：Vital herb-belt 505 (the 3rd generation) product causes rat stomach to glue absolute ethyl alcohol
Membrane damage has obvious protective function；By reagent 3.33g/kg dosage groups can reduce chronic ulcer rat model ulcer area, burst
Ulcer is shoaled and volume is reduced, and prompts by reagent to play the role of to promote the healing of Acetic chronic ulcer；By reagent is used model of spleen deficiency
The state of each dosage group animal is clearly better after medicine treatment, and its apparent condition is similar to blank control group, by reagent 6.667g/
Mouse weight caused by kg, 3.333g/kg group can alleviate rheum officinale declines, and can significantly improve model of spleen deficiency mouse temperature, hence it is evident that prolong
Long mouse low temperature swimming time, mice with spleen deficiency Intestinal propulsive rate can be substantially reduced, prompt vital herb-belt 505 (the 3rd generation) product
Mice spleen asthenic symptoms model of spleen deficiency sign can be improved, hence it is evident that enhancing model of spleen deficiency hardy fatigue resistance, can substantially reduce
Mice with spleen deficiency Intestinal propulsive rate, suppress intestinal motility hyperfunction, there is obvious function of invigorating spleen；By reagent 3.33g/kg,
1.665g/kg, 0.833g/kg can significantly reduce WBV value (200mpa.s, 30mpa.s, 5mpa.s, 1mpa.s), red thin
Born of the same parents' hematocrit, whole blood height cut relative indices, whole blood undercut relative indices, erythrocyte aggregation index, whole blood undercut reduced viscosity, whole blood
Height cuts reduced viscosity, erythrocyte mechanical fragility, deformable index TK, Casson viscosity, viscosity in yield stress and red blood cell
(P 0.01~P of ＜ ＜ 0.05), show that by reagent can significantly improve the hemorheological property of acute blood-stasis model rat, prompt 505 god
Work(primordial qi bag (the 3rd generation) product has obvious function of promoting blood circulation to disperse blood clots；By reagent 6.667g/kg and 3.333g/kg can obviously reduce
Mouse writhing number, by reagent 6.667g/kg and 3.333g/kg compared with the significantly raised pain threshold of control group, show 505 siddhi member
Airbag (the 3rd generation) product has obvious analgesic activity.Conclusion：Vital herb-belt 505 (the 3rd generation) product causes big to absolute ethyl alcohol
Mouse gastric mucosa damage has obvious protective function；Vital herb-belt 505 (the 3rd generation) product, which has, promotes the healing of Acetic chronic ulcer
Effect；Vital herb-belt 505 (the 3rd generation) product can improve the insufficiency of the spleen symptom of mouse, hence it is evident that suppress mice with spleen deficiency intestinal motility work(
Can be hyperfunction, there is obvious function of invigorating spleen；Vital herb-belt 505 (the 3rd generation) product can improve blood to acute blood-stasis model rat
Liquid stream is denatured, and has obvious function of promoting blood circulation to disperse blood clots；Vital herb-belt 505 (the 3rd generation) product has obvious analgesic activity.Rat rises
Dosage 0.833g/kg is imitated, is 2.5 times of people's dosage.
Test objective：Vital herb-belt 505 (the 3rd generation) product is the external application medicines and health protection by developing meticulously for many years
Articles for use, the clinical more than two decades tested achieve good curative effect.Its function is：Warming spleen and stomach for dispelling cold, promoting qi circulation and relieving pain, invigorating spleen and reinforcing stomach.For
Deficiency-cold in spleen and stomach, stagnation of cold in middle-JIAO, abdominal distention, crymodynia, deficiency of food, loose stool etc. caused by qi depression to blood stasis.In order to inquire into the effect of we
Mechanism, this research cause rat chronic gastric mucosa damage and rheum officinale to cause mice spleen ethanol induced Acute gastric mucosa damage, Dichlorodiphenyl Acetate
The influence of virtual model, the influence to acute blood-stasis model rat blood rheological characteristic and analgesic test further verify that its pharmacology is made
With providing experimental basis for clinical practice of the present invention.
1st, test material
1.1 equipment
Electronic balance：Model：AL104, accuracy：0.1mg, METTLERTOLEDO are produced.
YP1201N electronic balance (scale division values：0.1g), precision instrument Co., Ltd in Shanghai produces.
Nikon biomicroscope：Nikon companies produce.
Omron (Omron) electronic thermometer MC-106B, the Liao Dynasty's food medicine prison tool (standard) word 2003 the 2200013rd, Omron
Industry and trade (Dalian) Co., Ltd produces.
YLS-6A intelligence hot-plate instrument factory numbers：004060129 Shandong Academy of Medical Sciences's equipment station.
The full-automatic blood rheology test instruments of ZL4500i, model：ZL4500i, Beijing crowd speed great achievement.
The analytical instrument factory of 721 type spectrophotometer Shanghai the 3rd.
1.2 reagent
The preparation of rhubarb preparation：Rhubarb 1000g is taken, frozen water soaks 24h, filtering after section, and 80 DEG C of water-baths are condensed into
Per milliliter contained 1g.
It is phenol red：The new middle laboratory in Shanghai.
Black ink：Sincerely believe in fine preparation factory in Beijing.
Sodium hydroxide：Tianjin Heng Xing chemical reagent Manufacturing Co., Ltd.
Yellow Jackets, specification：25g/ bottles, lot number：100808, the term of validity：2015.8, German Merck import packing.Examination
3% (g/100ml) Nembutal sodium solution is configured to purified water when testing, for Animal Anesthesia.
Picric acid, Zhong Cheng Chemical Co., Ltd.s of Taishan City, specification：25g/ bottles, lot number：20080501, compound method：Weigh
1.50g picric acid, 50ml purified waters are added, shaking makes dissolving, sterilizes, produces 3% picric acid solution.
Adrenalin hydrochloride parenteral solution：Chinese medicines quasi-word H31021062, lot number：10140402, it is limited by Shanghai He Feng pharmacy
Company produces.Specification：1ml：1mg, date of manufacture：20140414, the term of validity：2016/04/13.
1.3 animal
KM mouse, body weight 18~22g, SD rat, 180~220 grams of body weight, tested by Xi'an Jiaotong University Medical College dynamic
Thing center provides, Quality of Experimental Animals credit number：SCXK (Shan) 2012-003.1.4 positive drug
Stomach Kang Ling particles：Heilongjiang Kuihua Yaoye Co.Ltd Co., Ltd, Chinese medicines quasi-word Z20090010, lot number：
20120564, the date of manufacture：20120523, valid until：2015.04 01, specification：4 grams × 10 bags, major function：Soft liver and
Stomach, dissipate stasis of blood hemostasis, relieving spasm to stop pain, remove the rotten and let fresh grow.Coerced for the gastral cavity pain caused by liver-stomach disharmony, obstruction of collaterals by blood stasis, company and two,
Gas, pantothenic acid；Acute and chronic gastritis, taste-blindness rate, gastrorrhagia are shown in above-mentioned patient.Usage and dosage：Boiling water is taken after mixing it with water, once
1 bag, 3 times a day.One after each meal.
Xiangsha liujun pill：LanZhou FoCi Pharmacy Co., Ltd, Chinese medicines quasi-word Z62020809, lot number 13C5-1, production
Date：2013-07-26, valid until 2018-07-25.Specification：Every 8 ball is equivalent to 3 grams of crude drug in whole.Major function：QI invigorating is good for
Spleen, and stomach.For the insufficiency of the spleen stagnation of the circulation of vital energy, indigestion, eructation and poor appetite, abdominal fullness and distention, diarrhea with loose stool.Usage and dosage：Orally, one time 12
Ball, 3 times a day.Fufang Danshen Pian：Guangzhou Baiyunshan Heji Huangpu Chinese Medicine Co., Ltd. produces, Chinese medicines quasi-word：Z44023372,
Lot number：G4A017, date of manufacture：2014.07.11 valid until：2016.06.Specification：Every weight 0.32g, major function：It is living
Blood stagnation resolvation, regulating qi-flowing for relieving pain.For the obstruction of qi in the chest of caused by energy stagnation and blood stasis, symptoms include uncomfortable in chest, pareordia shouting pain；Coronary disease and angina pectoris is shown in above-mentioned
Patient.Usage and dosage：Orally, one time 3,3 times a day.
Indomethacin tablets (indocin), the production of Linfen Qi Lin pharmaceutcal corporation, Ltds, authentication code：Chinese medicines quasi-word
H14020549, specification：Lot number：A131106, date of manufacture：20131118, valid until：20160517, every 25mg.
Benzylpenicillin sodium for injection：Lukang Medical Co., Ltd., Shandong produces, the quasi- H37020079 of traditional Chinese medicines, lot number：
L121214, the term of validity：2014/12/10, specification：0.48g (800,000 unit).
1.5 by reagent：Vital herb-belt 505 (the 3rd generation) product, hereinafter referred to as by reagent
Unit is provided：Xianyang Atiage Inst., Shaanxi.
Lot number：20140301.
Specification：20g/ medicine cores.
Major function：Warming spleen and stomach for dispelling cold, promoting qi circulation and relieving pain, invigorating spleen and reinforcing stomach.Draw for deficiency-cold in spleen and stomach, stagnation of cold in middle-JIAO, qi depression to blood stasis
Rise abdominal distention, crymodynia, deficiency of food, loose stool etc..
Usage and dosage：External application SHENQUE acupoint, is close to skin-worn.Every bag (medicine core) can be used continuously/wear 4~5 weeks.
Medication of 1.6 by reagents to animal subject
By reagent is made up of bafta packaging medicine powder, palpus " external application SHENQUE acupoint, be close to skin-worn " when human body uses,
In view of experimental animal and the difference of human body, and ensure the phase same sex of the by reagent on experimental animal to the full extent, therefore test
It is middle to be handled using following methods.
1.6.1 animal subject Skin sensitization test
1.6.1.1 rat skin preparation hair cutter is by rat abdomen ventrimeson and both sides unhairing, and unhairing scope about 6 ×
7cm2, check skin of unhairing within 24 hours after unhairing, the rat of skin damage rejected.
1.6.2.2 mouse skin preparation hair cutter is by mouse web portion ventrimeson and both sides unhairing, and unhairing scope about 2 ×
2cm2, check skin of unhairing within 24 hours after unhairing, the mouse of skin damage rejected.
1.6.2 SHENQUE acupoint point selection method
1.6.2.1 rat SHENQUE acupoint point selection method, by xiphoid-process cartilage point to the midpoint of the line at upper margin of pubic bone midpoint
For SHENQUE acupoint.
1.6.2.2 mouse SHENQUE acupoint point selection method, mouse chest and abdomen are taken to hit exactly, 3/ on superior border of manubrium to external genital organs on line
4 with lower 1/4 point of interface.
2nd, test method and result
Dosage is set：For people's body weight based on 60kg, by reagent people dosage is 0.333g/kg, according to the national 〇 mono- of Bureau of Drugs Supervision two
On April 23rd, 2 is printed and distributed in 9 healthcare function evaluation methods《There is assistant protection function evaluation method to gastric mucosa damage
Pilot project, experiment principle and result judgement》It is required that determine that the large, medium and small dosage of rat is 3.33g/kg, 1.665g/ through prerun
Kg, 0.833g/kg, be respectively equivalent to clinical people's dosage 10,5,2.5 times.The large, medium and small dosage of mouse be 6.667g/kg,
3.333g/kg, 1.665g/kg, be respectively equivalent to clinical people's dosage 20,10,5 times.
2.1 vital herb-belt 505s (the 3rd generation) influence of product to ethanol induced Acute gastric mucosa damage
Test method：SD rats 60, female, 180~220 grams of body weight, are randomly divided into 6 groups, every group 10.1st group empty
White control group and the 2nd group model control group are affixed on medical proof fabric with hospital gauze and are fixed on animal belly hair removal section SHENQUE acupoint,
And fixed (all herewith method fixes administration for following experiment) with non-stimulated adhesive tape；3rd group of positive controls (Kang Ling, stomach
Grain), the 24th day starts gastric infusion stomach Kang Ling particle 2.0g/kg, and once a day, successive administration 7 days, the 4th~6 group is by reagent
Large, medium and small dosage group, by reagent is wrapped up by 3.33g/kg, 1.665g/kg, 0.833g/kg dosage with hospital gauze, is affixed on
Animal SHENQUE acupoint is fixed on medical proof fabric, inspection is paid attention to during administration, avoids animal from baiting so that by reagent comes off, such as once
It was found that same dose by reagent is taken to be retightened by this law；After each dosage group SHENQUE acupoint of by reagent is administered 30 days, all animals are tight
Lattice fasting 24 hours (can't help water), also forbid giving by reagent during this.In addition to blank control group, all test group animals are given
Absolute ethyl alcohol 1.0ml/ only, puts to death animal, the complete stomach of exposure, ligatures pylorus, irrigate appropriate 10% formalin after 1 hour, Gu
Determine 20min, then cut off along greater curvature, clean gastric content, deploy gastric mucosa, with vernier caliper measurement blutpunkte or bleeding band
Length and width.Because the seriousness damaged representated by width is big far beyond length, therefore double integration.Its standards of grading is shown in Table 1.
Observation index：Each experimental group gastric mucosa damage degree is pressed down with the rate of injury (%), lesion extent index and damage
Rate processed represents.There is rat quantity/this group of rat quantity × 100% of bleeding or ulcer in the rate of injury (%)=certain group；Damage
Hinder integral exponential=group Injury score summation/group size of animal；Damaging inhibiting rate (%)=(A-B)/A × 100%, (A, B distinguish
For the lesion extent of model group and test group).
The acute alcohol of table 1 damage naked eyes, which are seen, looks into standards of grading
Pathologic examination and scoring：Gross examination is finished, and the position that every gastric mucosa of animal is damaged to most serious is cut
Under, it is fixed on 10% formalin, conventional film-making, HE dyeing, Microscopic observation.The positive cross section of attentional selection gastric mucosa, including it is viscous
The regional observation of film holostrome.
Methods of marking：It is divided into 5 in the degree of involving of whole mucous epithelium layer with hyperemia, bleeding, mucomembranous cell degeneration necrosis
Level.Congested weight is 1, and bleeding weight is 2, and epithelial cell degeneration necrosis weight is 3, and standards of grading and lesion total mark formula are shown in
Table 2.Acute gastric mucosal lesion visually observes appraisal result and is shown in Table 3.
Standards of grading under the acute gastric mucosal lesion mirror of table 2
The acute alcohol of table 3 damage naked eyes, which are seen, looks into appraisal result
Note：Compared with blank control group：△△P ＜ 0.01；Compared with model control group：*P ＜ 0.05,**P ＜ 0.01.
From table 3：Compared with model control group, by reagent 3.33g/kg, 1.665g/kg dosage group and positive controls
(stomach Kang Ling particles) can significantly mitigate absolute ethyl alcohol cause acute gastric mucosal injury lesion extent index (P ＜ 0.05~
0.01), by reagent 3.33g/kg, 1.665g/kg, 0.833g/kg dosage groups can obviously reduce the rate of injury, 3.33g/kg,
1.665g/kg dosage groups simultaneously can significantly improve damage inhibiting rate.
Histopathologic examination's result：Blank control group gastric epithelial cell form no abnormality seen changes, and lamina propria, glues
Film muscle layer, submucosa, muscle layer and serous coat are without cell infiltration, and gastric gland is without pathological changes such as atrophy, hyperplasia, denaturation.Model pair
It is obvious according to group mucous hyperemia, part epithelium denaturation, necrosis or come off, the obvious oedema of submucosa.Each medication group and model comparison
Group more has clear improvement, and visible surface cell is complete under mirror, smooth mucosal, accidental mottled superficial injury, substantially without filling
Blood, oedema.Pathological score the results are shown in Table 4 under acute gastric mucosal lesion mirror.
From table 4：Compared with model control group, the large, medium and small dosage of by reagent (3.33g/kg, 1.665g/kg,
0.833g/kg) it can substantially reduce absolute ethyl alcohol with positive control (stomach Kang Ling particles) group and cause acute gastric mucosal injury
Lesion total mark index (P ＜ 0.05~0.01).
Prompting：Vital herb-belt 505 (the 3rd generation) product causes rat pipe film injury to have obvious protection to make to absolute ethyl alcohol
With.
Pathological score result under the acute gastric mucosal lesion mirror of table 4
| Group | Dosage (g/kg) | Number of animals (only) | Lesion total mark |
| Blank control group | - | 10 | 0.1±0.3 |
| Model control group | - | 10 | 3.8±1.1△△ |
| Positive controls | 2.0 | 10 | 0.6±0.5** |
| Vital herb-belt 505 | 3.333 | 10 | 0.7±0.5** |
| Vital herb-belt 505 | 1.665 | 10 | 1.0±0.7** |
| Vital herb-belt 505 | 0.833 | 10 | 1.2±0.9** |
Note：Compared with blank control group：△△P ＜ 0.01；Compared with model control group：*P ＜ 0.05,**P ＜ 0.01.
2.2 vital herb-belt 505s (the 3rd generation) product causes the influence of chronic gastric mucosa damage to rat acetic acid
Test method, SD rats 60, male, 180~220 grams of body weight, animal fasting be can't help into water 24 hours, with 1%
Implement laparotomy ventrotomy after yellow Jackets (30mg/kg) anesthesia, sterilize belly, cut abdominal cavity under xiphoid-process, stomach is gently pulled out into abdomen
Outside chamber, 30ul 30% glacial acetic acid is injected under serous coat at stomach pylorus with micro syringe, stomach is gently sent back to, suturing them flesh
Layer, then skin suture, postoperative every rat muscle injection benzylpenicillin sodium for injection 4000u, once a day, is continuously injected 4 days,
Postoperative diet and water.The good animal of postoperative state was randomly divided into model control group, positive control by body weight in 5th day
Group (stomach Kang Ling particle 2.0g/kg), the large, medium and small dosage group of by reagent, every group 10.First group is model control group with medical
Gauze, which is affixed on medical proof fabric, is fixed on animal SHENQUE acupoint, second group of positive controls, and the 8th day starts Kang Ling, gastric infusion stomach
Grain 2.0g/kg, once a day, successive administration 7 days, the 3rd~5 group is the large, medium and small dosage group of by reagent, and by reagent is pressed
3.33g/kg, 1.665g/kg, 0.833g/kg dosage are wrapped up with hospital gauze, are affixed on medical proof fabric and are fixed on animal Shenque
Cave, inspection is paid attention to during administration, avoid animal from baiting so that by reagent comes off, such as one it is found that take same dose by reagent by this
Method is retightened；After each dosage group SHENQUE acupoint of by reagent is administered 14 days, the strict fasting of all animals 24 hours (can't help water), the 15th
It puts to death animal, cuts open the belly and takes stomach, takes out whole stomach and be soaked in 10% formaldehyde, and immersion is cut off after 20 minutes along greater curvature, is washed
Net content, take glandular stomach district expansion to be laid on glass plate, the moisture in ulcer is blotted with paper, measure ulcer area and use water filling
Method measures volume, and it the results are shown in Table 5.
Gastric ulcer area and Plethysmometry：The transverse diameter of ulcer surface and vertical footpath are measured, calculates ulcer area, calculation formula is
S=π × (d1/2) × (d2/2), S are ulcer area, and π is pi, and d1 is that the maximum measured by ulcer center indulges footpath, d2
For the maximum transverse diameter measured by ulcer center.Then colored ink is injected in ulcer with micro syringe, ulcer is filled up
It is concordant with periphery, read the volume that micro syringe high scale is ulcer.
Table 5 causes the effect of chronic gastric mucosa damage to rat acetic acid
Note：Compared with model control group：*P ＜ 0.05,**P ＜ 0.01.
From table 5：Compared with model control group, by reagent heavy dose group (3.33g/kg) and positive control (stomach Kang Ling
Particle) group can reduce this rat model ulcer area, ulcer shoals and volume reduces (P ＜ 0.05~0.01), in, it is low dose of
Group (1.665g/kg, 0.833g/kg) also has the trend for reducing ulcer area and reducing ulcer volume, but not statistically significant.
Prompting：Vital herb-belt 505 (the 3rd generation) product plays the role of to promote the healing of Acetic chronic ulcer.
2.3 vital herb-belt 505s (the 3rd generation) influence of product to mice with spleen deficiency
Test method, cleaning grade kunming mice 72 is taken, male, 18~22g of body weight, be randomly divided into 6 groups, respectively blank
Control group, model of spleen deficiency group, positive controls, the large, medium and small dosage group of by reagent, every group 12.Except blank control group mouse fills
Outside stomach deionized water, remaining 5 groups of mouse gavaging 100% rhubarb decocting liquid 30ml/kg, 1 time a day, cause within continuous 7 days insufficiency of the spleen
Model.Start to be administered within 8th day, blank control group is affixed on medical proof fabric with model of spleen deficiency group with hospital gauze is fixed on animal god
Fault cave, the 3rd group of positive controls, gavage xiangsha liujun pill 2.029g/kg (equivalent to 20 times of clinical people's dosage), 1 time a day；
4th~6 group is the large, medium and small dosage group of by reagent, and by reagent is used by 6.667g/kg, 3.333g/kg, 1.665g/kg dosage
Hospital gauze wrap up, be affixed on medical proof fabric and be fixed on animal SHENQUE acupoint, during administration pay attention to inspection, avoid animal from baiting so that
By reagent comes off, and such as one it is found that take same dose by reagent to retighten (following experiment all herewith method) by this law, and continuous 8
My god, the 4th day of administration and the 8th day, claim mouse weight respectively and measure mouse anus temperature with Omron electric body-temperature.Last dose
Water 12h, 0.5h after administration are can't help in preceding fasting, claim mouse weight, Omron electric body-temperature measurement anus temperature, survey frozen water swimming time,
Take thymus gland, spleen to claim weight in wet base and calculate organ coefficient.As a result：
2.3.1 to the influence of mice with spleen deficiency symptom
It is filthy that mouse after rheum officinale gavages occurs loose stool, crissum on the 5th~6 day, becomes thin, prolapse of the anus occur in Some Animals,
The performances such as hogback, indigestion and loss of appetite, few dynamic, weakness of limbs, hair are uninteresting, chilly, weight loss, kiss substantially with syndrome of spleen deficiency clinical manifestation
Close.2~3d after drug treatment, medication group mice with spleen deficiency recover shaping stool, and loose stool, prolapse of the anus situation are obviously improved, and activity increases
It is more, treat the 4th day various symptoms and disappear, mouse mode of appearance is shown in Table 6.
Influence of the table 6 to mice with spleen deficiency mode of appearance
| Group | Dosage (g/kg) | Hair color | Stool | Active situation |
| Blank control group | - | Color is bright | Shaping is active | Vivaciously |
| Model control group | - | Hair is withered, speckles with excreta | Loose stool | Activity is not liked, is flocked together |
| Positive controls | 2.029 | Hair color is bright | Compared with shaping | Vivaciously |
| Vital herb-belt 505 | 6.667 | Hair color is bright | Compared with shaping | Vivaciously |
| Vital herb-belt 505 | 3.333 | Hair color is bright | Compared with shaping | It is more active |
| Vital herb-belt 505 | 1.665 | Hair color is bright | Compared with shaping | It is more active |
From table 6：Positive control (xiangsha liujun pill) is organized and the equal energy of by reagent 6.667g/kg, 3.333g/kg treatment group
It is obviously improved the symptom of mice with spleen deficiency.
2.3.2 mouse weight and Temperature changing
0.5h after being administered is administered the 4th day and the 8th day, claims mouse weight, the results are shown in Table 7, is measured with Omron electric body-temperature
Anus temperature, the results are shown in Table 8.
Table 7 is administered front and rear model of spleen deficiency mouse weight and changes situation
Note：Compared with blank control group：△△P ＜ 0.01；Compared with model control group：*P ＜ 0.05,**P ＜ 0.01.By table
7 is visible：Positive control (xiangsha liujun pill) is organized and by reagent 6.667g/kg, 3.333g/kg treatment group animal the 4th day, the 8th day
Body weight rebound significantly, significance (the P ＜ 0.05~0.01) compared with model group.Prompting by reagent can improve mice with spleen deficiency disease
Disease, accelerate its weight recovery.From table 8：Model of spleen deficiency control group mice body temperature is significantly lower than blank control group, XIANGSHALIUJUNZI
Ball group and by reagent 6.667g/kg treatment groups mouse the 4th day and the 8th day body temperature are compared with model control group rebound significantly, and by reagent
The 8th day body temperature of 3.333g/kg treatment groups mouse is compared with model control group rebound significantly (P ＜ 0.05~0.01).Prompting：505 siddhi
Primordial qi bag (the 3rd generation) product can be obviously improved mice with spleen deficiency illness, accelerate temperature recovery.
Influence of the table 8 to mouse temperature
Note：Compared with blank control group：△△P ＜ 0.01；Compared with model control group：*P ＜ 0.05,**P ＜ 0.01.
2.3.3 to the influence of mice with spleen deficiency anti-stress ability
After the 8th day mouse surveys body temperature, it is 15 DEG C ± 1 DEG C that water temperature will be put into after mouse tail heavy burden 2g sheet leads, the depth of water
In 15cm 50 × 30 × 20cm ponds, 1 every time, with mouse because of fatigue and head is submerged in water 3 times as index, record trip
Swim the time (min), compared with model control group, t inspections are carried out between group, the results are shown in Table 9.
Influence of the table 9 to mice with spleen deficiency anti-stress ability
| Group | Number of animals (only) | Dosage (g/kg) | Low temperature swimming time (min) |
| Blank control group | 12 | - | 9.87±2.21 |
| Model of spleen deficiency control group | 12 | - | 6.37±2.05△ |
| Xiangsha liujun pill group | 12 | 2.029 | 9.52±2.35** |
| Vital herb-belt 505 | 12 | 6.667 | 9.25±2.78** |
| Vital herb-belt 505 | 12 | 3.333 | 8.47±2.58* |
| Vital herb-belt 505 | 12 | 1.665 | 7.73±3.10 |
Note：Compared with blank control group：△P ＜ 0.05；Compared with model of spleen deficiency control group：*P ＜ 0.05.
From table 9：Model of spleen deficiency group substantially shortens (P ＜ 0.05) compared with blank control group mouse low temperature swimming time, by
Reagent 6.667g/kg, 3.333g/kg group and positive control (xiangsha liujun pill) group can be obviously prolonged model of spleen deficiency mouse low temperature
Swimming time (P ＜ 0.05), show that vital herb-belt 505 (the 3rd generation) product can be remarkably reinforced the fatigue resistance of mice with spleen deficiency,
There is obvious anti-stress effect.
2.3.4 to the influence of mice with spleen deficiency immune organ weight
In the 9th day, mouse cervical vertebra is taken off into neck and puts to death dissection, claims thymus gland, spleen weight in wet base, calculates organ coefficient (g/kg bodies
Weight).It the results are shown in Table 10.
The result of table 10 shows vital herb-belt 505 (the 3rd generation) the large, medium and small dosage of product and xiangsha liujun pill to insufficiency of the spleen
Model mice thymus index and spleen index have no significant effect.
Influence of the table 10 to thymus index, spleen index
| Group | Animal (only) | Dosage (g/kg) | Thymus index (g/kg) | Spleen index (g/kg) |
| Blank control group | 12 | - | 1.769±0.358 | 4.375±0.735 |
| Model of spleen deficiency group | 12 | - | 1.746±0.536 | 4.140±0.559 |
| Xiangsha liujun pill group | 12 | 2.029 | 1.835±0.594 | 4.452±0.371 |
| Vital herb-belt 505 | 12 | 6.667 | 1.754±0.656 | 4.200±0.880 |
| Vital herb-belt 505 | 12 | 3.333 | 1.740±0.532 | 4.490±0.878 |
| Vital herb-belt 505 | 12 | 1.665 | 1.500±0.429 | 4.039±0.825 |
2.3.5 to the influence of mice with spleen deficiency Small intestine advance activity
Test method, cleaning grade kunming mice 72 is taken, male, 18~22g of body weight, be randomly divided into 6 groups, packet, modeling
With administration with 2.3, before last dose fasting can't help water 24h, 1h after administration, with 0.1ml/ gavage each group animal of black ink,
After 30min, mouse cervical vertebra is taken off into neck and put to death, abdominal cavity is opened, separates mesenterium, clip upper end to pylorus, lower end to ileocecus
Intestinal tube.It is to promote length that the small intestine of taking-up, which is laid in the distance that measurement on meter ruler enters farthest from pylorus to charcoal footline, and is measured
The total length (pylorus to ileocaecal sphineter) of small intestine is taken, ink propulsive rate is calculated as follows.Intestinal propulsive rate (%)=charcoal end front end is with imprisoning
Distance/small intestine total length × 100% of door.Result of the test is shown in Table 11.
From table 11：By reagent 6.667g/kg, 3.333g/kg group and xiangsha liujun pill group are compared with model control group
Mice with spleen deficiency Intestinal propulsive rate can be substantially reduced, suppresses intestinal motility hyperfunction (P ＜ 0.05~0.01).
Prompting：Vital herb-belt 505 (the 3rd generation) product can substantially reduce mice with spleen deficiency Intestinal propulsive rate, suppress small intestine fortune
Dynamic hyperfunction.
Influence of the table 11 to mice with spleen deficiency Small intestine advance activity
| Group | Number of animals | Dosage (g/kg) | Propulsion rate (%) |
| Blank control group | 12 | - | 60.13±6.10 |
| Model control group | 12 | - | 74.03±8.84△△ |
| Xiangsha liujun pill group | 12 | 2.029 | 60.24±5.55** |
| Vital herb-belt 505 | 12 | 6.667 | 64.42±5.11** |
| Vital herb-belt 505 | 12 | 3.333 | 63.75±11.02* |
| Vital herb-belt 505 | 12 | 1.665 | 69.08±12.67 |
Note：Compared with blank control group：△△P ＜ 0.01；Compared with model control group：*P ＜ 0.05**P ＜ 0.01.
Shown by above-mentioned experiment：Vital herb-belt 505 (the 3rd generation) product can improve mice spleen asthenic symptoms model of spleen deficiency body
Sign, hence it is evident that enhancing model of spleen deficiency hardy fatigue resistance, can substantially reduce mice with spleen deficiency Intestinal propulsive rate, suppress small intestine fortune
Dynamic hyperfunction, there is obvious function of invigorating spleen.
2.4 vital herb-belt 505s (the 3rd generation) influence of product to acute blood-stasis model rat blood rheological characteristic
Test method, healthy SD rat 60 is taken, female, 180~220g of body weight, be randomly divided into 6 groups, respectively blank pair
According to group, blood stasis model control group, positive controls, the large, medium and small dosage group of by reagent.Blank control group is used with blood stasis model group
Hospital gauze, which is affixed on medical proof fabric, is fixed on animal SHENQUE acupoint；Positive controls, gavage Fufang Danshen Pian 0.48g/kg is (quite
In 10 times of clinical people's dosage), the 24th day starts gavage, 1 time a day, gavage 7 days；4th~6 group is large, medium and small dose of by reagent
Amount group, by reagent is wrapped up by 3.333g/kg, 1.665g/kg, 0.833g/kg dosage with hospital gauze, is affixed on medical proof fabric
Animal SHENQUE acupoint is fixed on, successive administration (pays attention to inspection, avoid animal from baiting so that by reagent comes off, such as 30 days during administration
One it is found that take same dose by reagent to be retightened by this law).1h after last dose, in addition to blank control group, model group, sun
Property control group, the large, medium and small dosage group of by reagent adrenalin hydrochloride 0.15mg/ is subcutaneously injected only, except sky after injection two hours
Outside white control group, above each group rat quickly immerses 5min in 4 DEG C of frozen water, the water on rat fur is dried after taking-up, two is small
When after in addition to blank control group, adrenalin hydrochloride 0.15mg/ is subcutaneously injected only in remaining each group again, and it is small that water 18 is can't help in fasting
When, with 10% urethane 1ml/100g intraperitoneal injection of anesthesia, arteria carotis communis is separated, intubation takes blood 5ml, blood is flowed into naturally containing few
In the test tube for measuring liquaemin, shaken in drainage, blood is not coagulated, detected on ZL4500i lectin from hemolymph analyzers (22 DEG C of room temperature,
37 DEG C of sample constant temperature), record blood rheology parameter and compare between group and examined with t, its result of the test be shown in Table 12, table 13,
Table 14, table 15, table 16.
Table 12 to acute blood-stasis model rat blood rheological characteristic influence (N=10)
Note：Compared with blank control group：△△P ＜ 0.01；Compared with model control group：**P ＜ 0.01.
Influence of the table 13 to acute blood-stasis model rat blood rheological characteristic
Note：Compared with blank control group：△P ＜ 0.05,△△P ＜ 0.01；Compared with model control group：*P ＜ 0.05,**P ＜
0.01。
Influence of the table 14 to acute blood-stasis model rat blood rheological characteristic
Note：Compared with blank control group：△△P ＜ 0.01；Compared with model control group：*P ＜ 0.05,**P ＜ 0.01.
Influence of the table 15 to acute blood-stasis model rat blood rheological characteristic
Note：Compared with blank control group：△△P ＜ 0.01；Compared with model control group：*P ＜ 0.05,**P ＜ 0.01.
Influence of the table 16 to acute blood-stasis model rat blood rheological characteristic
Note：Compared with blank control group：△P ＜ 0.05,△△P ＜ 0.01；Compared with model control group：*P ＜ 0.05,**P ＜
0.01。
From table 12, table 13, table 14, table 15, table 16：Model group WBV value (200mpa.s), WBV value
(30mpa.s), WBV value (5mpa.s), WBV value (1mpa.s), hematocrit, that whole blood height cuts relative indices, whole blood is low
Cut relative indices, erythrocyte aggregation index, whole blood undercut reduced viscosity, reduction viscosity of blood, erythrocyte mechanical fragility, red
Viscosity is obviously higher than blank control group (0.01~P of P ＜ in cytomorphosis index TK, Casson viscosity, yield stress and red blood cell
＜ 0.05), illustrate blood stasis model success；Positive control (Fufang Danshen Pian) organize and by reagent 3.33g/kg, 1.665g/kg,
0.833g/kg can significantly reduce WBV value (200mpa.s, 30mpa.s, 5mpa.s, 1mpa.s), packed cell volume, whole blood
Height, which cuts relative indices, whole blood undercut relative indices, erythrocyte aggregation index, whole blood undercut reduced viscosity, whole blood height and cuts to reduce, to be glued
Viscosity (0.01~P of P ＜ in degree, erythrocyte mechanical fragility, deformable index TK, Casson viscosity, yield stress and red blood cell
＜ 0.05), as a result show that vital herb-belt 505 (the 3rd generation) product has clear improvement the effect of hemorheological property.
2.5 vital herb-belt 505s (the 3rd generation) product causes the influence of mice pain to glacial acetic acid
Test method (bibliography[16,17]), mouse 60 is taken, male, 18~22g of body weight, is randomly divided into 5 groups, every group
12.1st group is that blank control group is affixed on medical proof fabric with hospital gauze and is fixed on animal SHENQUE acupoint；2nd group is positive right
According to group, gavage indocin 10mg/kg (experiment starts gavage on the 28th, even to 3 days)；3rd~5 group by 6.667g/kg,
3.333g/kg, 1.665g/kg dosage fix SHENQUE acupoint by reagent, continuous 30 days (inspection to be paid attention to during administration, avoids animal
Bait so that by reagent comes off, such as one it is found that take same dose by reagent to be retightened by this law).5 groups of the above is given in last
30min after medicine, 0.7% glacial acetic acid 0.2ml/ mouse are injected intraperitoneally, count mouse writhing number in 20 minutes (in belly contraction
Recessed, stretching, extension hind leg, buttocks are raised, crawling) and writhing response inhibiting rate and between group compare, t is examined, and it the results are shown in Table 17.
Writhing response inhibiting rate calculates：
Suppress percentage (%)=(the average writhing number of the average writhing number-administration group of blank control group)/blank control group to put down
Equal writhing number × 100%.
From table 17：By reagent 6.667g/kg, 3.333g/kg and Indomethacin tablets 10mg/kg can obviously reduce small
Mouse writhing number, show that vital herb-belt 505 (the 3rd generation) product has obvious analgesic activity.
The influence that table 17 is reacted mouse writhing
| Group | Number of animals | Dosage (g/kg) | Writhing number | Inhibiting rate (%) |
| Blank control group | 12 | - | 61.08±12.00 | |
| Indomethacin tablets group | 12 | 10mg/kg | 19.67±10.32** | 67.80 |
| Vital herb-belt 505 group | 12 | 6.667 | 46.08±10.13** | 24.56 |
| Vital herb-belt 505 group | 12 | 3.333 | 48.58±13.70* | 20.46 |
| Vital herb-belt 505 group | 12 | 1.665 | 51.08±15.75 | 16.37 |
Note：Compared with blank control group*P ＜ 0.05；**P ＜ 0.01.
The influence (hot plate method) that 2.6 vital herb-belt 505s (the 3rd generation) product is reacted mouse thermostimulation
Test method, mouse 70 is taken, female, 18~22g of body weight, female mice is placed in the YLS- of (55 ± 0.5 DEG C)
On 6A intelligence hot-plate instrument hot plates, record from its vola and contact hot plate to time (S, the hot plate response latency) work for occurring licking metapedes
For pain indicator.Determine normal pain threshold 2 times, the threshold of pain based on averaging, first reject response latency >=30s or≤5s or
Middle leaper, pain threshold is selected to be randomly divided into 5 groups by pain threshold, the 1st group is blank control group in 5 seconds to 30 seconds mouse 60
It is affixed on hospital gauze on medical proof fabric and is fixed on animal SHENQUE acupoint, and is fixed with non-stimulated adhesive tape；2nd group is positive right
According to group, gavage indocin 10mg/kg (experiment starts gavage on the 28th, even to 3 days)；3rd~5 group by 6.667g/kg,
3.333g/kg, 1.665g/kg dosage fix SHENQUE acupoint by reagent, continuous 30 days (inspection to be paid attention to during administration, avoids animal
Bait so that by reagent comes off, such as one it is found that take same dose by reagent to be retightened by this law).5 groups of the above is given in last
Mouse is placed on the hot plate of (55 ± 0.5) DEG C and determines tolerable pain by 30min, 60min, 90min, 120min, 180min after medicine
Time (if mouse puts 60s on hot plate and do not lick metapedes still, takes out mouse, in terms of 60s) immediately, and record tolerance time is carried out between group
Compare, t is examined, and it the results are shown in Table 18.
From table 18：30min~90min after the administration of indocin group, compared with the significantly raised pain threshold of control group (P ＜
0.01), continue to 120min (P ＜ 0.05), 30min~90min is significantly raised compared with control group after by reagent 6.667g/kg administrations
Pain threshold (P 0.01~P of ＜ ＜ 0.05), the significantly raised pain thresholds of 30min (P ＜ 0.05) after 3.333g/kg administrations.
Prompting：Vital herb-belt 505 (the 3rd generation) product has obvious analgesic activity to thermostimulation.
Influence that table 18 is reacted mouse thermostimulation (N=12)
Note：Compared with blank control group*P ＜ 0.05；**P ＜ 0.01.
3rd, conclusion
Vital herb-belt 505 (the 3rd generation) product causes acute gastric mucosal injury to have obvious protective function absolute ethyl alcohol；
Vital herb-belt 505 (the 3rd generation) product plays the role of to promote the healing of Acetic chronic ulcer；Vital herb-belt 505 (the 3rd generation) produces
Product can improve the insufficiency of the spleen symptom of mouse, and it is hyperfunction substantially to suppress mice with spleen deficiency intestinal motility, has substantially to model of spleen deficiency mouse
Function of invigorating spleen；Vital herb-belt 505 (the 3rd generation) product can improve hemorheological property to acute blood-stasis model rat, have obvious
Function of promoting blood circulation to disperse blood clots；Vital herb-belt 505 (the 3rd generation) product has obvious analgesic activity.Rat effective dose 0.833g/kg,
For 2.5 times of people's dosage.
Claims (4)
1. a kind of Chinese medicine composition for treating syndrome of deficient cold of spleen and stomach, it is characterised in that it is made of the raw material of following parts by weight
Medicament：
Dried orange peel 3-4 parts, banksia rose 2-3 parts, folium artemisiae argyi 9-12 parts, cloves 3-4 parts, Chinese cassia tree 3-4 parts, rhizoma zingiberis 2-3 parts, corydalis tuber 1-2 parts,
Fennel seeds 2-3 parts, rhizoma atractylodis 1-2 parts, evodia rutaecarpa 2-3 parts, Radix Angelicae Sinensis 2-3 parts, radix bupleuri 1-2 parts, frutus cnidii 1-2 parts, the root of straight ladybell 1-2 parts,
Honey-fried licorice root 3-4 parts.
2. a kind of Chinese medicine composition for treating syndrome of deficient cold of spleen and stomach according to claim 1, it is characterised in that it is by following
Medicament made of the raw material of parts by weight：
3 parts of dried orange peel, 2 parts of the banksia rose, 11 parts of folium artemisiae argyi, 3 parts of cloves, 3 parts of Chinese cassia tree, 2 parts of rhizoma zingiberis, 1 part of corydalis tuber, 2 parts of fennel seeds, rhizoma atractylodis
1 part, 2 parts of evodia rutaecarpa, 2 parts of Radix Angelicae Sinensis, 1 part of radix bupleuri, 1 part of frutus cnidii, 1 part of the root of straight ladybell, 3 parts of honey-fried licorice root.
A kind of 3. Chinese medicine composition for treating syndrome of deficient cold of spleen and stomach according to claim 1, it is characterised in that above-mentioned raw materials system
Into the formulation of medicament be all formulations for being included in pharmacy.
A kind of 4. preparation method of Chinese medicine composition for treating syndrome of deficient cold of spleen and stomach according to claim 3, it is characterised in that
Being prepared into the method and steps of powder preparation formulation is：
First, purification, processing processing：Dried orange peel, the banksia rose, folium artemisiae argyi, cloves, Chinese cassia tree, rhizoma zingiberis, corydalis tuber, fennel seeds, rhizoma atractylodis, evodia rutaecarpa,
Radix Angelicae Sinensis, radix bupleuri, frutus cnidii, the root of straight ladybell and honey-fried licorice root, purified, processing processing, dried 2 hours under 50~55 DEG C of low temperature, with
It is standby medicinal；
2nd, co-grinding is handled：Chinese cassia tree, the taste Chinese medicine material co-grinding of cloves 2 are into 24 mesh coarse powder；The banksia rose, honey-fried licorice root, rhizoma zingiberis,
Folium artemisiae argyi, the taste Chinese medicine material co-grinding of radix bupleuri 5 are into 24 mesh coarse powder；After the thick medicinal powder of both the above is mixed with Radix Angelicae Sinensis, fennel seeds, snake
Machine tool, evodia rutaecarpa, corydalis tuber, the root of straight ladybell, dried orange peel, the taste co-grinding of rhizoma atractylodis 8 sieving, produce mixed powder into 80 mesh fine powders；
3rd, disinfect：Take mixed powder in step 2 by 20 grams of dosimetric systems into medicine core, assembling, plastic packaging,60Co radiation sterilizations,
Packaging, is produced.
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| CN105709199A (en) * | 2016-04-13 | 2016-06-29 | 李晓枫 | Traditional Chinese medicine composition for treating stomach cold and stomachache |
| CN107184933A (en) * | 2017-05-25 | 2017-09-22 | 张广顺 | A kind of Chinese medicine composition for treating the spleen-stomach diseases |
| CN112870307A (en) * | 2021-01-16 | 2021-06-01 | 梧州市中医医院 | Formula of warm middle-jiao ironing powder and production equipment thereof |
| CN116509824B (en) * | 2023-06-28 | 2023-09-15 | 中国人民解放军总医院第六医学中心 | Traditional Chinese medicine gel and preparation process thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1092314A (en) * | 1993-03-13 | 1994-09-21 | 邢春生 | Treatment diarrheal Chinese medicine for outer use |
| CN201020147Y (en) * | 2007-03-07 | 2008-02-13 | 甘璐 | Bellybutton-warming pain-relieving belt |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1092314A (en) * | 1993-03-13 | 1994-09-21 | 邢春生 | Treatment diarrheal Chinese medicine for outer use |
| CN201020147Y (en) * | 2007-03-07 | 2008-02-13 | 甘璐 | Bellybutton-warming pain-relieving belt |
Non-Patent Citations (2)
| Title |
|---|
| 四逆散合平胃散加减治疗慢性胃炎46例;王耀;《实用中医药杂志》;20100615;第26卷(第6期);第388-389页 * |
| 慢性胃炎从痰论治体会;陈福如;《新中医》;19950615(第6期);第62-63页 * |
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