CN104611382A - Method for preparing high value-added products by virtue of hydrolysis and fermentation of waste yeast - Google Patents

Method for preparing high value-added products by virtue of hydrolysis and fermentation of waste yeast Download PDF

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Publication number
CN104611382A
CN104611382A CN201510076555.9A CN201510076555A CN104611382A CN 104611382 A CN104611382 A CN 104611382A CN 201510076555 A CN201510076555 A CN 201510076555A CN 104611382 A CN104611382 A CN 104611382A
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China
Prior art keywords
yeast
fermentation
high value
waste yeast
hydrolysis
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Pending
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CN201510076555.9A
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Chinese (zh)
Inventor
陈新德
黄超
熊莲
陈雪芳
李小妹
张海荣
林晓清
郭海军
王璨
王波
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Guangzhou Institute of Energy Conversion of CAS
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Guangzhou Institute of Energy Conversion of CAS
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Priority to CN201510076555.9A priority Critical patent/CN104611382A/en
Publication of CN104611382A publication Critical patent/CN104611382A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a method for preparing high value-added products by virtue of hydrolysis and fermentation of waste yeast. The method comprises the following steps: a, hydrolyzing the waste yeast in a reaction kettle at a solid-liquid ratio of 1-15 by taking an acid having the mass fraction of 0.05-5% as a catalyst; b, separating the solid residue (obtained after hydrolysis in the step a) from the hydrolysate; c, adding an alkaline substance to neutralize the hydrolysate until the pH meets the requirement of fermentation, and filtering to obtain a culture medium for subsequent fermentation; d, adding different strains to the culture medium obtained in the step c for fermentation, thereby obtaining different high value-added products. According to the method, the industrial waste yeast is converted into the hydrolysate rich in fermentable sugar by virtue of dilute-acid hydrolysis, and then various high value-added products such as biobutanol, microbial oil and bacterial cellulose are prepared by virtue of fermentation, and finally, the waste material is changed into things of value and the problem of low utilization rate of the industrial waste yeast is overcome; the method has great economic benefit and environmental benefit.

Description

A kind of waste yeast hydrolysed ferment prepares the method for high value added product
Technical field:
The present invention relates to biological chemical field, be specifically related to a kind of method that waste yeast hydrolysed ferment prepares high value added product.
Background technology:
Yeast is a kind of important industrial microorganism, and along with the development of yeast industry, waste yeast is on the increase.If directly waste yeast is abandoned, the huge wasting of resources can be caused, also pollute the environment simultaneously.High value added product prepared by comprehensive utilization Industry Waste yeast not only can increase economic benefit, can also solve its problem of environmental pollution.
Dilute acid hydrolysis is the conventional method for hydrolysis of biomass, Wood Adhesives from Biomass can be made to be for fermentable containing the hydrolyzed solution of a large amount of fermentable sugar by the mineral acid of lower concentration or organic acid.
As a rule, above-mentioned Industry Waste yeast contains a large amount of carbohydrate (based on zymosan), is therefore expected by technology for hydrolyzing, and the hydrolyzed solution obtained containing a large amount of fermentable sugar is used for fermentation, thus realize turning waste into wealth, make Biological resources be able to efficiency utilization.
Current, more industry applied by yeast is wine brewing industry, usually can produce a large amount of useless yeast saccharomyces cerevisiaes after zymamsis; And along with the extensive application of microbial oil on light industry and food, derived energy chemical, grease also can produce a large amount of waste oil yeast after extracting.
Summary of the invention:
The object of this invention is to provide a kind of method that waste yeast hydrolysed ferment prepares high value added product.
The present invention is achieved by the following technical programs:
Waste yeast hydrolysed ferment prepares a method for high value added product, and the method comprises the following steps:
A, hydrolysis: utilize massfraction be the acid of 0.05 ~ 5% as catalyzer, to waste yeast thalline with 1 ~ 15 solid-to-liquid ratio in a kettle. 70 ~ 230 DEG C hydrolysis 10 ~ 360min;
B, solid-liquid separation: be separated with hydrolyzed solution by the solid residue after centrifugal or filtration make step a be hydrolyzed;
C, neutralization: add alkaline matter neutralizing hydrolysis liquid, make pH reach the requirement of fermentation, filters the substratum obtaining subsequent fermentation;
D, fermentation: in the substratum that step c obtains, add different strains ferment, prepare different high added value chemicals.
Described waste yeast derives from the discarded yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) in wine industry and the discarded rhodotorula glutinis (Rhodotorula glutinis) in microbial oil industry, light white latent ball yeast (Cryptococcus albidus), trichosporon cutaneum (Trichosporon cutaneum), separate sub-sieve yeast (Yarrowia lipolytica) of fat, any one in Trichosporondermatis or Trichosporn coremiiforme.
Alkaline matter described in step c be selected from lime, calcium carbonate, sodium hydroxide, potassium hydroxide, sodium carbonate, salt of wormwood one or more.
Bacterial strain described in steps d is selected from the clostridium acetobutylicum (Clostridium acetobutylicum) producing biological butanol, produce the yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) of bio-ethanol, the bacterial strain rhodotorula glutinis (Rhodotorula glutinis) of producing microbial grease, light white latent ball yeast (Cryptococcus albidus), trichosporon cutaneum (Trichosporoncutaneum), separate sub-sieve yeast (Yarrowia lipolytica) of fat, Trichosporon dermatis or Trichosporncoremiiforme, produce the conventional bacterial strain of bacteria cellulose as acetobacter xylinum (Acetobacter xylinum), described in described high added value chemicals, high added value chemicals is selected from biological butanol, microbial oil, any one in bacteria cellulose.
Beneficial effect of the present invention is as follows: the present invention makes Industry Waste yeast conversion be the hydrolyzed solution being rich in fermentable sugar by dilute acid hydrolysis, and then by fermentation for all kinds of high value added product as biological butanol, microbial oil, bacteria cellulose etc., final realization " is turned waste into wealth ", overcome owing to lacking the low problem of Industry Waste yeast availability that suitable deep processing technology causes, there is very large economic benefit and environmental benefit.
Embodiment:
Below further illustrate of the present invention, instead of limitation of the present invention.
Embodiment 1:
With the useless yeast saccharomyces cerevisiae after industrial production for raw material, take sulfuric acid as catalyzer, sour massfraction is 0.05%, and solid-to-liquid ratio is 1, and temperature of reaction is 230 DEG C, and the reaction times is 10min.Use lime to regulate pH to 6.5 by the hydrolyzed solution obtained after filtration solid-liquid separation, filter the substratum obtaining subsequent fermentation.Add clostridium acetobutylicum in the medium and carry out anaerobically fermenting product biological butanol, after fermentation, butanols output is 5g/L.
Embodiment 2:
With the useless trichosporon cutaneum after industrial production for raw material, take sulfuric acid as catalyzer, sour massfraction is 0.5%, and solid-to-liquid ratio is 1, and temperature of reaction is 120 DEG C, and the reaction times is 100min.Use lime to regulate pH to 6.5 by the hydrolyzed solution obtained after filtration solid-liquid separation, filter the substratum obtaining subsequent fermentation.Add yeast saccharomyces cerevisiae in the medium and carry out anaerobically fermenting product bio-ethanol, after fermentation, ethanol production is 4g/L.
Embodiment 3:
With the useless trichosporon cutaneum after industrial production for raw material, take hydrochloric acid as catalyzer, sour massfraction is 3%, and solid-to-liquid ratio is 5, and temperature of reaction is 70 DEG C, and the reaction times is 360min.The hydrolyzed solution obtained after being separated by centrifugal solid-liquid uses calcium carbonate to regulate pH to 6.0, filters the substratum obtaining subsequent fermentation.Add rhodotorula glutinis in the medium and carry out aerobic fermentation producing microbial grease, after fermentation, grease yield is 4g/L.
Embodiment 4:
With the useless Trichosporn coremiiforme after industrial production for raw material, take formic acid as catalyzer, sour massfraction is 5%, and solid-to-liquid ratio is 15, and temperature of reaction is 120 DEG C, and the reaction times is 120min.The hydrolyzed solution obtained after being separated by centrifugal solid-liquid uses sodium hydroxide to regulate pH to 6.5, filters the substratum obtaining subsequent fermentation.Add Trichosporondermatis in the medium and carry out aerobic fermentation producing microbial grease, after fermentation, grease yield is 5g/L.
Embodiment 5:
With the sub-sieve yeast of the useless solution fat after industrial production for raw material, take acetic acid as catalyzer, sour massfraction is 4%, and solid-to-liquid ratio is 10, and temperature of reaction is 130 DEG C, and the reaction times is 100min.Use potassium hydroxide to regulate pH to 7.5 by the hydrolyzed solution obtained after filtration solid-liquid separation, filter the substratum obtaining subsequent fermentation.Add light white latent ball yeast in the medium and carry out aerobic fermentation producing microbial grease, after fermentation, grease yield is 5g/L.
Embodiment 6:
With the useless light white latent ball yeast after industrial production for raw material, take sulfuric acid as catalyzer, sour massfraction is 1%, and solid-to-liquid ratio is 8, and temperature of reaction is 130 DEG C, and the reaction times is 150min.The hydrolyzed solution obtained after being separated by centrifugal solid-liquid uses sodium carbonate to regulate pH to 7.0, filters the substratum obtaining subsequent fermentation.Add Trichosporn coremiiforme in the medium and carry out aerobic fermentation producing microbial grease, after fermentation, grease yield is 3.5g/L.
Embodiment 7:
With the useless Trichosporon dermatis after industrial production for raw material, take sulfuric acid as catalyzer, sour massfraction is 1.5%, and solid-to-liquid ratio is 12, and temperature of reaction is 110 DEG C, and the reaction times is 180min.The hydrolyzed solution obtained after being separated by centrifugal solid-liquid uses salt of wormwood to regulate pH to 6.5, filters the substratum obtaining subsequent fermentation.Add acetobacter xylinum aerobic fermentation in the medium and produce bacteria cellulose, after fermentation, bacteria cellulose output is 2g/L.
Embodiment 8:
With the useless rhodotorula glutinis after industrial production for raw material, take sulfuric acid as catalyzer, sour massfraction is 2%, and solid-to-liquid ratio is 8, and temperature of reaction is 115 DEG C, and the reaction times is 120min.Use lime to regulate pH to 7.0 by the hydrolyzed solution obtained after filtration solid-liquid separation, filter the substratum obtaining subsequent fermentation.Add trichosporon cutaneum in the medium and carry out aerobic fermentation producing microbial grease, after fermentation, grease yield is 5.5g/L.

Claims (4)

1. waste yeast hydrolysed ferment prepares a method for high value added product, it is characterized in that, the method comprises the following steps:
A, utilize massfraction be the acid of 0.05 ~ 5% as catalyzer, to waste yeast thalline with 1 ~ 15 solid-to-liquid ratio in a kettle. 70 ~ 230 DEG C hydrolysis 10 ~ 360min;
B, by centrifugal or filter make step a be hydrolyzed after solid residue be separated with hydrolyzed solution;
C, add alkaline matter neutralizing hydrolysis liquid, make pH reach the requirement of fermentation, filter the substratum obtaining subsequent fermentation;
Add different strains in d, the substratum that obtains in step c to ferment, prepare different high added value chemicals.
2. waste yeast hydrolysed ferment according to claim 1 prepares the method for high value added product, it is characterized in that, described waste yeast derives from any one in the discarded yeast saccharomyces cerevisiae in wine industry and the discarded rhodotorula glutinis in microbial oil industry, light white latent ball yeast, trichosporon cutaneum, the sub-sieve yeast of solution fat, Trichosporon dermatis or Trichosporn coremiiforme.
3. waste yeast hydrolysed ferment according to claim 1 prepares the method for high value added product, it is characterized in that, alkaline matter described in step c be selected from lime, calcium carbonate, sodium hydroxide, potassium hydroxide, sodium carbonate, salt of wormwood one or more.
4. waste yeast hydrolysed ferment according to claim 1 prepares the method for high value added product, it is characterized in that, bacterial strain described in steps d is selected from the clostridium acetobutylicum producing biological butanol, produce the yeast saccharomyces cerevisiae of bio-ethanol, the bacterial strain rhodotorula glutinis of producing microbial grease, light white latent ball yeast, trichosporon cutaneum, separate the sub-sieve yeast of fat, Trichosporondermatis or Trichosporn coremiiforme, acetobacter xylinum (Acetobacter xylinum), described high added value chemicals is selected from biological butanol, microbial oil, any one in bacteria cellulose.
CN201510076555.9A 2015-02-12 2015-02-12 Method for preparing high value-added products by virtue of hydrolysis and fermentation of waste yeast Pending CN104611382A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105821094A (en) * 2016-04-21 2016-08-03 中国科学院青岛生物能源与过程研究所 Preparation method and application of nanoscale bacterial cellulose membrane
CN109055441A (en) * 2018-08-30 2018-12-21 江南大学 A method of butanol is produced using Pichia pastoris solid-state castoff high-efficiency fermenting

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004108609A1 (en) * 2003-06-06 2004-12-16 Forskningscenter Risø Fermentation media comprising wastewater and use hereof
CN102031275A (en) * 2010-11-19 2011-04-27 山东福田药业有限公司 Method for preparing yeast cream by using waste yeast

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004108609A1 (en) * 2003-06-06 2004-12-16 Forskningscenter Risø Fermentation media comprising wastewater and use hereof
CN102031275A (en) * 2010-11-19 2011-04-27 山东福田药业有限公司 Method for preparing yeast cream by using waste yeast

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BYUNG-GON RYU ET AL.: "Use of organic waste from the brewery industry for high-density cultivation of the docosahexaenoic acid-rich microalga, Aurantiochytrium sp. KRS101", 《BIORESOURCE TECHNOLOGY》 *
丁满生等: "啤酒酵母泥回收利用研究动态", 《酿酒科技》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105821094A (en) * 2016-04-21 2016-08-03 中国科学院青岛生物能源与过程研究所 Preparation method and application of nanoscale bacterial cellulose membrane
CN109055441A (en) * 2018-08-30 2018-12-21 江南大学 A method of butanol is produced using Pichia pastoris solid-state castoff high-efficiency fermenting

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