CN104611372A - Method for preparing special peanut cake powder for fermentation - Google Patents
Method for preparing special peanut cake powder for fermentation Download PDFInfo
- Publication number
- CN104611372A CN104611372A CN201510049622.8A CN201510049622A CN104611372A CN 104611372 A CN104611372 A CN 104611372A CN 201510049622 A CN201510049622 A CN 201510049622A CN 104611372 A CN104611372 A CN 104611372A
- Authority
- CN
- China
- Prior art keywords
- groundnut meal
- fermentation
- peanut cake
- enzyme
- special
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Seeds, Soups, And Other Foods (AREA)
Abstract
The invention relates to a method for preparing special peanut cake powder for fermentation. The method comprises the following steps of: taking non-mildew peanut cake, crushing, passing a 40-mesh mesh sieve, and taking sieved materials to obtain the peanut cake powder; mixing the peanut cake powder and an industrial enzyme preparation, conveying into a double-screw extruding and bulking machine after mixing uniformly, extruding and bulking; drying extruded and bulked materials, and enabling the water content of the materials to be less than 8-12%; and crushing the dried materials by a micro-powder machine while controlling the temperature to be below 80 DEG C in the crushing process to obtain the special peanut cake powder for fermentation. The method has the advantages that not only is the problem for the need of a nitrogen-source culture medium with comprehensive nutrition in industrial fermentation solved and is the need for normal metabolic and synthetic products of microorganisms in the industrial fermentation process met, but also the special culture medium for fermentation can also provide proper precursor substances, coenzymes or cofactors and the like for forming the metabolic products, so that the yield of metabolites is increased.
Description
Technical field
The present invention relates to a kind of method preparing the special groundnut meal that ferments, belong to the preparation method field of industrial fermentation nitrogen source medium.
Background technology
Current industrial fermentation, particularly antibiotic fermentation groundnut meal as main in Streptomycin sulphate, erythromycin etc. is as the major nitrogen source of substratum, traditional groundnut meal is that the peanut cake produced by high temperature oil expression is simply pulverized and obtains, due to the Semen arachidis hypogaeae protein serious degenerative contained in the peanut cake that squeezes through high temperature, form a large amount of insoluble protein aggregate, cause its deliquescent decline simultaneously, then affect the emulsifying property of Semen arachidis hypogaeae protein, whipability and gelation etc. and nutritive property.How to develop a kind of nutritive ingredient comprehensively to ferment special groundnut meal, the needs meeting industrial fermentation nitrogen source medium have been problem demanding prompt solution in current fermentation industry.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method preparing the special groundnut meal that ferments, the present invention fully combines zymolysis technique and screw extrusion technology carries out modification to groundnut meal, thus prepare the special groundnut meal of a kind of fermentation, not only solve the needs of problems to the nitrogen source medium of comprehensive nutrition in industrial fermentation, meet the needs of microorganism eubolism and synthetic product in industrial fermentation processes, and this fermentation special culture media can provide suitable precursor substance for the formation of meta-bolites again, coenzyme or cofactor etc., thus the output that improve metabolite is provided.
The technical scheme that the present invention solves the problems of the technologies described above is as follows: a kind of method preparing the special groundnut meal that ferments, comprising:
1) getting the peanut cake without going mouldy, pulverizing and passing through 40 order mesh screens, peanut cake reaches 100%, extracting screen underflow to the percent of pass of 40 order mesh screens after pulverizing, and obtains groundnut meal;
2) by 1) groundnut meal that obtains mixes with industrial enzyme preparation, mixes rear feeding twin-screw extruder, extruding puffing;
3) raw material dry 2) after extruding puffing, makes the water content of raw material be less than 8 ~ 12%;
4) by 3) dried raw material pulverizes through pulverizing mill, and crushing process temperature controls, below 80 DEG C, to obtain the special groundnut meal that ferments.
On the basis of technique scheme, the present invention can also do following improvement.
Further, 1) in, described peanut cake is that non-transgenic peanut produces through high temperature oil expression, and its protein content massfraction is greater than 46%, and lipid content massfraction is greater than 6%.
Further, 2) in, described industrial enzyme preparation is Alcalase enzyme, Protemax enzyme and Papain enzyme, Alcalase enzyme dosage is 0.05 ~ 0.15% of groundnut meal weight, Protemax enzyme dosage is 0.05 ~ 0.15%, Papain enzyme dosage of groundnut meal weight is 0.1 ~ 0.2% of groundnut meal weight.
The beneficial effect of above-mentioned steps is adopted to be according to the application principle of controlled enzymatic hydrolysis technology in protein-modified, simultaneously according to substratum sterilization principle in fermenting process, protease A lcalase, Protemax and Papain is added in groundnut meal making processes, determining protease A lcalase, Protemax and Papain addition in groundnut meal according to experimental data by controlling groundnut meal protein hydrolysis degree (DH value), making the enzymolysis of groundnut meal occur in the sterilizing process of substratum.In groundnut meal due to high temperature squeezing the Semen arachidis hypogaeae protein of serious degenerative through the inscribe of protease A lcalase, Protemax and Papain or circumscribed effect, make proteolysis or the depolymerization of sex change, thus reach the object of protein structure or composition modification, and then cause the change of protein physico-chemical property, ultimately improve the functional performance of Semen arachidis hypogaeae protein.Through controlled enzymatic hydrolysis, obtained groundnut meal can not only meet the needs of microorganism eubolism and synthetic product in industrial fermentation processes, suitable precursor substance, coenzyme or cofactor etc. can be provided for the formation of meta-bolites again, thus improve the output of meta-bolites.
Further, 2) in, the temperature of described extruding puffing is 80 ~ 150 DEG C, and pressure is 0.8 ~ 1.5MPa.
The beneficial effect of this step is adopted to be that material is under the promotion of extruder screw, tortuous advance, through overdraft, mixing, pressurized heating under the effect of frictional force and propulsive force, a large amount of energy is put aside in groundnut meal, along with pressure reduces, these huge energy of savings are able to rapid release, in groundnut meal due to high temperature squeezing and the Semen arachidis hypogaeae protein of high concentration at this moment by abundant depolymerization, volume also expands thereupon, water-solublely to be greatly improved, greatly to facilitate the effect of proteolytic enzyme, also improve the utilising efficiency of microorganism to substratum simultaneously.
Further, 3) in, described drying adopts air stream drying technology, is taken away by the moisture in raw material with 60 ~ 80 DEG C of dry airs.
Further, 4) in, described in be crushed to groundnut meal particle diameter be 3 ~ 5 μm.
The invention has the beneficial effects as follows:
First point, present method technique is simple, easy to operate, is easy to automatization, low cost of manufacture;
Second point, by the Semen arachidis hypogaeae protein in the special groundnut meal of the fermentation of present method gained by biological enzymolysis and physical exfoliation technology, its functional performance is not only made to be improved fully, meet the needs of microorganism eubolism and synthetic product in industrial fermentation processes, and the special groundnut meal of this fermentation can provide suitable precursor substance, coenzyme or cofactor etc. for the formation of meta-bolites again, thus improve the output of metabolite, improve the overall economic efficiency of industrial fermentation enterprise.
The output of metabolite is different because of different microbiotic, and as erythromycin, fermentation Special flower fecula output increased 1-2% compared with prior art (simply pulverizing) peanut powder, unit fermented liquid is tired and improved 8-10%.For another example bacitracin, fermentation Special flower fecula output increased 2-3% compared with prior art (simply pulverizing) peanut powder, unit fermented liquid is tired and is improved 10-15%.
Embodiment
Be described principle of the present invention and feature below, example, only for explaining the present invention, is not intended to limit scope of the present invention.
In following embodiment, the enzyme activity of Alcalase used is 200000u/ml, and Pangbo Bioengineering Co Ltd, Nanning's production is also commercially available.
The enzyme activity of Protemax is 100000u/ml, and Pangbo Bioengineering Co Ltd, Nanning's production is also commercially available.
The enzyme activity of Papain is 200000u/ml, and Pangbo Bioengineering Co Ltd, Nanning's production is also commercially available.
Embodiment 1
1) get the peanut cake without going mouldy, pulverize and pass through 40 order mesh screens, extracting screen underflow, obtains groundnut meal, and described peanut cake is that non-transgenic peanut produces through high temperature oil expression, and its protein content massfraction is greater than 46%, and lipid content massfraction is greater than 6%,
2) by 1) groundnut meal that obtains mixes with industrial enzyme preparation, described industrial enzyme preparation is Alcalase enzyme, Protemax enzyme and Papain enzyme, Alcalase enzyme dosage is 0.05% of groundnut meal weight, Protemax enzyme dosage is 0.05% of groundnut meal weight, Papain enzyme dosage is 0.1% of groundnut meal weight, mixes rear feeding twin-screw extruder, extruding puffing, the temperature of described extruding puffing is 80 DEG C, and pressure is 0.8MPa.
3) air stream drying 2 is adopted) raw material after extruding puffing, with 60 DEG C of dry airs, the moisture in raw material is taken away, make the water content of raw material be less than 8%,
4) by 3) to be crushed to groundnut meal particle diameter through pulverizing mill be 3 μm to dried raw material, crushing process temperature controls, below 80 DEG C, to obtain the special groundnut meal that ferments.
By the fermentation of above-mentioned steps gained special peanut cake amyloid proteins content >=46%, fat content >=6%, moisture≤8%, ash content≤7%.
Embodiment 2
1) get the peanut cake without going mouldy, pulverize and pass through 40 order mesh screens, extracting screen underflow, obtains groundnut meal, and described peanut cake is that non-transgenic peanut produces through high temperature oil expression, and its protein content massfraction is greater than 46%, and lipid content massfraction is greater than 6%,
2) by 1) groundnut meal that obtains mixes with industrial enzyme preparation, described industrial enzyme preparation is Alcalase enzyme, Protemax enzyme and Papain enzyme, Alcalase enzyme dosage is 0.1% of groundnut meal weight, Protemax enzyme dosage is 0.1%, Papain enzyme dosage of groundnut meal weight is 0.15% of groundnut meal weight, mixes rear feeding twin-screw extruder, extruding puffing, the temperature of described extruding puffing is 120 DEG C, and pressure is 1MPa
3) air stream drying 2 is adopted) raw material after extruding puffing, with 70 DEG C of dry airs, the moisture in raw material is taken away, make the water content of raw material be less than 10%,
4) by 3) to be crushed to groundnut meal particle diameter through pulverizing mill be 4 μm to dried raw material, crushing process temperature controls, below 80 DEG C, to obtain the special groundnut meal that ferments.
By the fermentation of above-mentioned steps gained special peanut cake amyloid proteins content >=46%, fat content >=6%, moisture≤10%, ash content≤7%.
Embodiment 3
1) get the peanut cake without going mouldy, pulverize and pass through 40 order mesh screens, extracting screen underflow, obtains groundnut meal; Described peanut cake is that non-transgenic peanut produces through high temperature oil expression, and its protein content massfraction is greater than 46%, and lipid content massfraction is greater than 6%,
2) by 1) groundnut meal that obtains mixes with industrial enzyme preparation, described industrial enzyme preparation is Alcalase enzyme, Protemax enzyme and Papain enzyme, Alcalase enzyme dosage is 0.15% of groundnut meal weight, Protemax enzyme dosage is 0.15%, Papain enzyme dosage of groundnut meal weight is 0.2% of groundnut meal weight, mixes rear feeding twin-screw extruder, extruding puffing, the temperature of described extruding puffing is 150 DEG C, and pressure is 1.5MPa
3) air stream drying 2 is adopted) raw material after extruding puffing, with 80 DEG C of dry airs, the moisture in raw material is taken away, make the water content of raw material be less than 12%,
4) by 3) to be crushed to groundnut meal particle diameter through pulverizing mill be 5 μm to dried raw material, crushing process temperature controls, below 80 DEG C, to obtain the special groundnut meal that ferments.
By the fermentation of above-mentioned steps gained special peanut cake amyloid proteins content >=46%, fat content >=6%, moisture≤12%, ash content≤7%.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (6)
1. prepare a method for the special groundnut meal that ferments, it is characterized in that, comprising:
1) get the peanut cake without going mouldy, pulverize and pass through 40 order mesh screens, extracting screen underflow, obtains groundnut meal;
2) by 1) groundnut meal that obtains mixes with industrial enzyme preparation, mixes rear feeding twin-screw extruder, extruding puffing;
3) raw material dry 2) after extruding puffing, makes the water content of raw material be less than 8 ~ 12%;
4) by 3) dried raw material pulverizes through pulverizing mill, and crushing process temperature controls, below 80 DEG C, to obtain the special groundnut meal that ferments.
2. method according to claim 1, is characterized in that, 1) in, described peanut cake is that non-transgenic peanut produces through high temperature oil expression, and its protein content massfraction is greater than 46%, and lipid content massfraction is greater than 6%.
3. method according to claim 1 and 2, it is characterized in that, 2) in, described industrial enzyme preparation is Alcalase enzyme, Protemax enzyme and Papain enzyme, Alcalase enzyme dosage is 0.05 ~ 0.15% of groundnut meal weight, Protemax enzyme dosage is 0.05 ~ 0.15%, Papain enzyme dosage of groundnut meal weight is 0.1 ~ 0.2% of groundnut meal weight.
4. method according to claim 3, is characterized in that, 2) in, the temperature of described extruding puffing is 80 ~ 150 DEG C, and pressure is 0.8 ~ 1.5MPa.
5. the method according to claim 1 or 4, is characterized in that, 3) in, described drying adopts air stream drying technology, is taken away by the moisture in raw material with 60 ~ 80 DEG C of dry airs.
6. method according to claim 5, is characterized in that, 4) in, described in be crushed to groundnut meal particle diameter be 3 ~ 5 μm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510049622.8A CN104611372A (en) | 2015-01-30 | 2015-01-30 | Method for preparing special peanut cake powder for fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510049622.8A CN104611372A (en) | 2015-01-30 | 2015-01-30 | Method for preparing special peanut cake powder for fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104611372A true CN104611372A (en) | 2015-05-13 |
Family
ID=53146022
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510049622.8A Pending CN104611372A (en) | 2015-01-30 | 2015-01-30 | Method for preparing special peanut cake powder for fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104611372A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105462837A (en) * | 2015-12-31 | 2016-04-06 | 青岛海科生物技术有限公司 | Composite organic nitrogen source and preparation method thereof |
| CN107254425A (en) * | 2017-08-04 | 2017-10-17 | 河南振新生物技术股份有限公司 | A kind of bacillus subtilis bacterium culture medium and preparation method thereof |
| CN112715600A (en) * | 2020-12-31 | 2021-04-30 | 广东广益科技实业有限公司 | Preparation method and preparation device of powder cake emulsifying and foaming agent |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102911993A (en) * | 2012-10-18 | 2013-02-06 | 山东理工大学 | Preparation of protein and polypeptide through low-temperature extrusion enzymolysis method |
| CN102952840A (en) * | 2012-08-29 | 2013-03-06 | 吉林大学 | Method for rapidly improving enzymolysis effect of corn gluten meal |
| CN103202457A (en) * | 2013-04-15 | 2013-07-17 | 江南大学 | Method for preparing soy sauce by extruding and processing soy sauce residue resource |
| CN103340353A (en) * | 2013-07-31 | 2013-10-09 | 北京工商大学 | Instant highland barley flour processing technology |
-
2015
- 2015-01-30 CN CN201510049622.8A patent/CN104611372A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102952840A (en) * | 2012-08-29 | 2013-03-06 | 吉林大学 | Method for rapidly improving enzymolysis effect of corn gluten meal |
| CN102911993A (en) * | 2012-10-18 | 2013-02-06 | 山东理工大学 | Preparation of protein and polypeptide through low-temperature extrusion enzymolysis method |
| CN103202457A (en) * | 2013-04-15 | 2013-07-17 | 江南大学 | Method for preparing soy sauce by extruding and processing soy sauce residue resource |
| CN103340353A (en) * | 2013-07-31 | 2013-10-09 | 北京工商大学 | Instant highland barley flour processing technology |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105462837A (en) * | 2015-12-31 | 2016-04-06 | 青岛海科生物技术有限公司 | Composite organic nitrogen source and preparation method thereof |
| CN107254425A (en) * | 2017-08-04 | 2017-10-17 | 河南振新生物技术股份有限公司 | A kind of bacillus subtilis bacterium culture medium and preparation method thereof |
| CN112715600A (en) * | 2020-12-31 | 2021-04-30 | 广东广益科技实业有限公司 | Preparation method and preparation device of powder cake emulsifying and foaming agent |
| CN112715600B (en) * | 2020-12-31 | 2024-01-05 | 广东广益科技实业有限公司 | Preparation method and preparation device of powder cake emulsifying foaming agent |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102845677B (en) | Preparation method of pre-enzymatic oat powder and application of preparation method | |
| Li et al. | Simultaneous saccharification and fermentation of broken rice: an enzymatic extrusion liquefaction pretreatment for Chinese rice wine production | |
| JP5024848B2 (en) | Method of converting biomass into solidified material and method of using the solidified material | |
| CN105011346A (en) | Method for preparing reconstituted cut tobacco sheets | |
| CN100469256C (en) | Method for preparing yeast peptide utilizing beer yeast | |
| CN101019623A (en) | Making process of fermented plant fiber and protein food | |
| CN104611372A (en) | Method for preparing special peanut cake powder for fermentation | |
| CN103981015B (en) | Semen Flos Carthami cold pressing oil method | |
| CN105211616A (en) | A kind of method of comprehensive utilization to shrimping beam trawl | |
| CN104611395B (en) | A kind of method for preparing fermentation special-purpose maize flour | |
| CN103652327B (en) | Biological feed produced by wastes | |
| CN107259103A (en) | Orange peel residue fermented feed production technology | |
| CN104690067A (en) | Recycling utilization method for energy grass and livestock manures | |
| CN102370100B (en) | Method for producing expanded pet feed by using banana lees | |
| CN109971798A (en) | A method of producing high concentration lactic acid | |
| CN106879873A (en) | A kind of prawn feed and its preparation technology | |
| CN105483159B (en) | Preparation method of special long-shaped rice flour for fermentation | |
| CN112964050B (en) | Method and equipment for quickly drying single-cell protein powder at low temperature | |
| CN111607539B (en) | Chinese cabbage extract culture medium and method for producing gamma-aminobutyric acid through fermentation | |
| CN105462837A (en) | Composite organic nitrogen source and preparation method thereof | |
| CN113973980A (en) | Super-large particle size buoyancy fermentation soft pellet feed | |
| CN106671474B (en) | Improve the method and device of compressed biomass ratio of briquetting | |
| CN101704901B (en) | Method for separating Tara bean gum from Tara beans | |
| CN111296620A (en) | Method for preparing high-dispersity soybean protein | |
| CN105695373A (en) | Bacillus subtilis(natto) fermentation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150513 |
|
| RJ01 | Rejection of invention patent application after publication |