CN104611257A - Preparation method of bacillus licheniformis preparations for drinking water of livestock and poultry - Google Patents
Preparation method of bacillus licheniformis preparations for drinking water of livestock and poultry Download PDFInfo
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Abstract
The invention discloses a preparation method of bacillus licheniformis preparations for drinking water of livestock and poultry. The preparation comprises a liquid preparation and a solid preparation; the preparation method comprises the steps of using an appropriate enlarge cultivation method to obtain bacillus licheniformis fermentation liquor, and then preparing the fermentation liquor to obtain the liquid preparation and the solid preparation. According to the preparation method of the bacillus licheniformis preparations for the drinking water of livestock and poultry, the bacillus licheniformis liquid preparation and the bacillus licheniformis solid preparation are better than the bacillus licheniformis powder produced by the conventional fermentation method in water solubility and mobility, and can be used for promoting the growth of beneficial microorganisms (lactobacillus and bifidobacterium) and inhibiting propagation of harmful microorganisms (escherichia coli) in intestinal tracts of a weaned pig, so as to improve the growth performance of the weaned pig and control the diarrhea rate.
Description
Technical field
The invention belongs to additive formulations field, relate to the preparation process of Bacillus licheniformis probiotic bacterium---the Bacillus licheniformis of liquid fermenting is made the liquid or solid preparation for livestock and fowl drinking water.
Background technology
Bacillus licheniformis (Bacillus licheniformis) is considered to one of effective commerical prod of livestock and poultry cultivation height, one of main probiotic bacterium kind that the approval of the Ministry of Agriculture of Ye Shi China uses.
Bacillus licheniformis can produce multiple enzyme, there is stronger proteolytic enzyme, amylase and lipase activity, go back the enzyme of complex carbohydrates in degradable plant feed simultaneously, as polygalacturonase, dextranase, cellulase etc., wherein a lot of enzyme is the enzyme that Mammals and bird can not be synthesized in vivo, thus promotes animal digesting and assimilating nutritive substance.
The spore that Bacillus licheniformis produces is can be strong in Direct-Fed Microbials in poultry feed
novelone of kind.It can be granulated by steam; can grow in enteron aisle; consume oxygen; cause the anaerobic environment of more favourable ancestral home milk-acid bacteria and bifidus bacillus; be conducive to these ancestral homes and the beneficial bacteria amount reproduction of bacterium colony can be generated at intestinal mucosa; produce organic acid, suppress pathogenic bacteria, thus protection animal and bird intestines is healthy and improve poultry production performance.
Feeding Bacillus licheniformis product exists with spore form, useful effect is played to animal capable, the vegetative cell with metabolic activity can be sprouted in animal digestive tract front portion, and there is the ability stablizing animal intestinal microflora, in digestive tube, Bacillus licheniformis quantity constantly increases energy antagonism animal pathogen, safeguards and the regulating intestinal canal eubiosis.
Feeding Bacillus licheniformis can produce multiple nutrients material as VITAMIN, amino acid, somatomedin etc., participates in the metabolism of body, and can produce lactic acid, can improve the utilization of animal to calcium, phosphorus, iron, promotes the absorption of vitamins D.
The liquid fermentation process of Bacillus licheniformis is ripe, has been subjected to applies widely and play good effect as fodder additives.But, in cultivation site, often need add Bacillus licheniformis in livestock and fowl drinking water system, to improve livestock and poultry cultivation effect.But, because conventional lichens bacillus preparation auxiliary material, fermentation impurities etc. are too much, add in livestock and fowl drinking water system, drinking-water nipple can be blocked because of Impurity deposition wherein, time serious, cause line clogging, be thus unwell to drinking-water and add.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of production technique of Bacillus licheniformis preparation, overcome that the substrate occurred in conventional fermentation process is too much, impurity too much, the problem of livestock and fowl drinking water interpolation cannot be carried out, to reach cultivation site object easy to use.
The preparation method of a kind of Bacillus licheniformis preparation for livestock and fowl drinking water of the present invention, comprises the preparation method of liquid preparation and solid preparation, wherein,
The preparation method of liquid preparation comprises the steps:
1a) getting appropriate Bacillus licheniformis is inoculated in the triangular flask including substratum I, cultivates, obtain first order seed in cultivation shaking table;
2a) get the first extractor, and drop into the medium ii of preparation, regulate pH to carry out boiling after 7.0, digested liquid is staticly settled, supernatant liquid is transferred to seed fermenter;
3a) by step 2a) seed fermenter in after supernatant liquid carries out high-temperature sterilization, drop into step 1a) first order seed, enlarged culturing obtains Bacillus licheniformis liquid;
4a) get the second extractor, and drop into the medium ii of preparation, regulate pH to carry out boiling after 7.0, digested liquid is staticly settled, supernatant liquid is transferred to fermentor tank;
5a) by step 4a) fermentor tank in after supernatant liquor carries out high-temperature sterilization, drop into step 3a) Bacillus licheniformis liquid, fermentation culture is until gemma transformation efficiency reaches after more than 95%, stop fermentation, qualification Bacillus licheniformis viable count, and to adjust pH be 3.5-4.0, obtain fermented liquid;
6a) according to detection level and target product content, determine Dilution ratio, to step 5a) fermented liquid in filling sterilized water, after 100 eye mesh screens filter, to be produced into Bacillus licheniformis level be 1.0 × 10
10the liquid preparation of cfu/g;
The preparation method of solid preparation comprises the steps:
1b) getting appropriate Bacillus licheniformis is inoculated in the triangular flask including substratum I, cultivates, obtain first order seed in cultivation shaking table;
2b) get the first extractor, and drop into the medium ii of preparation, regulate pH to carry out boiling after 7.0, digested liquid is staticly settled, supernatant liquid is transferred to seed fermenter;
3b) by step 2b) seed fermenter in after supernatant liquid carries out high-temperature sterilization, drop into step 1b) first order seed, enlarged culturing obtains Bacillus licheniformis liquid;
4b) get the second extractor, and drop into the medium ii of preparation, regulate pH to carry out boiling after 7.0, digested liquid is staticly settled, supernatant liquid is transferred to fermentor tank;
5b) by step 4b) fermentor tank in after supernatant liquor carries out high-temperature sterilization, drop into step 3b) Bacillus licheniformis liquid, fermentation culture is until gemma transformation efficiency reaches after more than 95%, stop fermentation, qualification Bacillus licheniformis viable count, and to adjust pH be 3.5-4.0, obtain fermented liquid;
6b) by step 5b) fermented liquid filter with 100 eye mesh screens after obtain clear liquid, according to detection limit and solid-to-liquid ratio, centrifugal and concentrated after dropping into the mixing of solubility auxiliary material, then obtaining Bacillus licheniformis level through spraying dry is 2.0 × 10
11the solid solubility preparation of cfu/g.
Further, described step 1a) and step 1b) Bacillus licheniformis be
preservationnumber be the Bacillus licheniformis of CGMCC No.9385, this Bacillus licheniformis liquid hold-up is 1.5 × 10
10cfu/g.
Further, described step 1a) and step 1b) substratum I be beef extract-peptone Shake flask medium, the pH for high-temperature sterilization is 7.0 and includes 5g/L extractum carnis, the water base substratum of 10g/L peptone and 5g/L sodium-chlor; In described cultivation shaking table, incubation time is 16-20h.
Further, described step 2a) and step 2b) medium ii be fermention medium, the pH for high-temperature sterilization is 7.0 and includes the water base substratum of 5g/L swelling soya dreg, 12g/L Semen Maydis powder, 10g/L W-Gum, 5g/L calcium chloride, 10g/L yeast extract, 1g/L potassium primary phosphate, 0.05g/L manganous sulfate, 1.8g/L magnesium sulfate, 10g/L corn steep liquor, 20g/L wheat bran and 1g/L soya-bean oil.
Further, described step 2a), step 4a), step 2b) and step 4b) conditions of cooking be temperature 100 DEG C, time 1-3h, sedimentation time is 3-5h.
Further, described step 3a) and step 3b) in when dropping into first order seed, seed fermenter temperature is 37 DEG C, and tank pressure is 0.01-0.02Mpa; Described enlarged culturing condition is temperature 37-39 DEG C, time 18-20h, stirs, air flow 10-12m3/h.
Further, described step 5a) and step 5b) in drop into Bacillus licheniformis liquid be 150L; When dropping into Bacillus licheniformis liquid, fermentation jar temperature is 37 DEG C, and tank pressure is 0.01-0.02Mpa; Described culture condition is temperature 37-39 DEG C, time 16-24h, stirs, air flow 800-1000m3/h.
Further, described step 1a) and step 1b) triangular flask content be 5L, in-built 2L substratum I; Described step 2a) and step 2b) the first extractor capacity be 500L, in-built 300L medium ii; Described step 4a) and step 4b) the second extractor capacity be 20T, in-built 15T medium ii.
Further, described step 6b) solubility auxiliary material is dextrin; The container of described centrifugal employing is disk centrifugal separator; Described concentrated condition is the 30-35% being concentrated into original volume; Described spraying dry is with intake air temperature 200-220 DEG C in spray-drying tower, and air outlet temperature 90-100 DEG C is carried out spraying dry.
Further, described high-temperature sterilization condition is temperature 121-128 DEG C, time 30-35min.
The present invention's Bacillus licheniformis liquid used
preservationnumber be: CGMCC No.9385, unit is: Chinese microorganism strain
preservationmanagement committee's common micro-organisms center, address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, and the time is: on June 25th, 2014, name is called: Bacillus licheniformis Bacillus licheniformis.
Bacillus licheniformis liquid preparation prepared by the preparation method of a kind of Bacillus licheniformis preparation for livestock and fowl drinking water of the present invention and solid preparation have Bacillus licheniformis the pulvis better water-soluble and mobility of producing compared with normal fermentation; And all can promote the growth of beneficial microorganism in weanling pig enteron aisle (lactobacillus and bifidus bacillus) and suppress the breeding of harmful microorganism (intestinal bacteria), improve the growth performance of weanling pig, and control diarrhea rate.
Embodiment
The technique means realized to make the present invention, creation characteristic, reaching object and effect is easy to understand, below in conjunction with specific embodiment, setting forth the present invention further.
The preparation method of a kind of Bacillus licheniformis preparation for livestock and fowl drinking water of the present embodiment, comprises the preparation method of liquid preparation and solid preparation, wherein,
The preparation method of liquid preparation comprises the steps:
1a) getting 3g concentration is 1.5 × 10
10the Bacillus licheniformis liquid of cfu/g (
preservationnumber for CGMCC No.9385) be inoculated in the 5L triangular flask including 2L substratum I, this substratum I is beef extract-peptone Shake flask medium, for including 5g/L extractum carnis, the water base substratum of 10g/L peptone and 5g/L sodium-chlor, afterwards regulate pH be 7.0 and at 121 DEG C high-temperature sterilization 30min, be put in again to cultivate in shaking table and cultivate 18h, obtain first order seed;
2a) amount of trying to please is 500L first extractor, and drop into the 300L medium ii of preparation, this medium ii is fermention medium, for including the water base substratum of 5g/L swelling soya dreg, 12g/L Semen Maydis powder, 10g/L W-Gum, 5g/L calcium chloride, 10g/L yeast extract, 1g/L potassium primary phosphate, 0.05g/L manganous sulfate, 1.8g/L magnesium sulfate, 10g/L corn steep liquor, 20g/L wheat bran and 1g/L soya-bean oil, pH is regulated to be 7.0 afterwards, be warming up to 100 DEG C and carry out boiling 2h, after liquid after boiling is staticly settled 4h, supernatant liquid is transferred to seed fermenter;
3a) by step 2a) seed fermenter in after supernatant liquid carries out high-temperature sterilization (121 DEG C) 30min, be cooled to 37 DEG C, control tank pressure is 0.01Mpa, drops into step 1a) first order seed that obtains, stirred liq at 37 DEG C also keeps air flow to be 10m
3under/h, enlarged culturing 18h obtains Bacillus licheniformis liquid;
4a) amount of trying to please is 20T second extractor, and drops into the 15T medium ii of preparation, and the compound method of medium ii is with step 2a), pH is regulated to be 7.0 afterwards, be warming up to 100 DEG C and carry out boiling 2h, after the liquid after boiling is staticly settled 4h, supernatant liquid is transferred to fermentor tank;
5a) by step 4a) fermentor tank in supernatant liquor carry out 121 DEG C at after high-temperature sterilization 30min, be cooled to 37 DEG C, control tank pressure is 0.01Mpa, drops into 150L step 3a) Bacillus licheniformis liquid, stirred liq at 37 DEG C also keeps air flow to be 800m
3/ h bottom fermentation cultivates 20h until gemma transformation efficiency reaches after more than 95%, stops fermentation, qualification Bacillus licheniformis viable count, and regulates pH to be 3.8, and obtaining Bacillus licheniformis level is 1.2 × 10
10-1.5 × 10
10the fermented liquid of cfu/g;
6a) according to detection level and target product content, determine Dilution ratio, to step 5a) fermented liquid in filling sterilized water, after 100 eye mesh screens filter, to be produced into Bacillus licheniformis level be 1.0 × 10
10the liquid preparation of cfu/g;
The preparation method of solid preparation comprises the steps:
1b) getting 3g concentration is 1.5 × 10
10the Bacillus licheniformis liquid of cfu/g (
preservationnumber for CGMCC No.9385) be inoculated in the 5L triangular flask including 2L substratum I, this substratum I is beef extract-peptone Shake flask medium, for including 5g/L extractum carnis, the water base substratum of 10g/L peptone and 5g/L sodium-chlor, the pH regulator of this water base substratum is after 7.0, high-temperature sterilization 30min at 121 DEG C, is put in afterwards to cultivate in shaking table and cultivates 18h, obtain first order seed;
2b) amount of trying to please is 500L first extractor, and drop into the 300L medium ii of preparation, this medium ii is fermention medium, for including the water base substratum of 5g/L swelling soya dreg, 12g/L Semen Maydis powder, 10g/L W-Gum, 5g/L calcium chloride, 10g/L yeast extract, 1g/L potassium primary phosphate, 0.05g/L manganous sulfate, 1.8g/L magnesium sulfate, 10g/L corn steep liquor, 20g/L wheat bran and 1g/L soya-bean oil, pH is regulated to be 7.0 afterwards, be warming up to 100 DEG C and carry out boiling 2h, after liquid after boiling is staticly settled 4h, supernatant liquid is transferred to seed fermenter;
3b) by step 2b) seed fermenter in supernatant liquid carry out 121 DEG C at after high-temperature sterilization 30min, be cooled to 37 DEG C, control tank pressure is 0.01Mpa, drops into step 1b) first order seed that obtains, stirred liq at 37 DEG C also keeps air flow to be 10m
3under/h, enlarged culturing 18h obtains Bacillus licheniformis liquid;
4b) amount of trying to please is 20T second extractor, and drops into the 15T medium ii of preparation, and the compound method of medium ii is with step 2b), pH is regulated to be 7.0 afterwards, be warming up to 100 DEG C and carry out boiling 2h, after the liquid after boiling is staticly settled 4h, supernatant liquid is transferred to fermentor tank;
5b) by step 4b) fermentor tank in supernatant liquor carry out 121 DEG C at after high-temperature sterilization 30min, be cooled to 37 DEG C, control tank pressure is 0.01Mpa, drops into 150L step 3b) Bacillus licheniformis liquid, stirred liq at 37 DEG C also keeps air flow to be 800m
3/ h bottom fermentation cultivates 20h until gemma transformation efficiency reaches after more than 95%, stops fermentation, qualification Bacillus licheniformis viable count, and regulates pH to be 3.8, and obtaining Bacillus licheniformis level is 1.2 × 10
10-1.5 × 10
10the fermented liquid of cfu/g;
6b) by step 5b) fermented liquid with 100 eye mesh screens filter after obtain clear liquid, according to detection limit and solid-to-liquid ratio, proceed to disk centrifugal separator after dropping into dextrin mixing carry out centrifugal and be concentrated into 30% of original volume, then enter with intake air temperature 200-220 DEG C in spray-drying tower, air outlet temperature 90-100 DEG C is carried out spraying dry to obtain Bacillus licheniformis level is 2.0 × 10
11the solid solubility preparation of cfu/g.
The Bacillus licheniformis liquid preparation obtained by the present embodiment and solid preparation are produced the high dense powder preparation of the Bacillus licheniformis solid obtained respectively and are carried out com-parison and analysis experiment with normal fermentation.
Experimental technique is: produced by normal fermentation 2.0 × 10
11the high dense powder preparation of cfu/g Bacillus licheniformis solid and the present embodiment prepare 1.0 × 10
10cfu/g liquid preparation and 2.0 × 10
11cfu/g solid preparation product is all configured to containing 1.0 × 10
10the aqueous solution of cfu/100g, leaves standstill 24h, by measured weight after solid residue drying, observes the adhesivity of three simultaneously.Experimental result is shown in
table 1.
table 1the Bacillus licheniformis preparation Performance comparision of different preparation method
table 1result show: the high dense powder preparation of Bacillus licheniformis solid that the Bacillus licheniformis liquid preparation that the present embodiment obtains and the solvability of solid preparation in water are produced significantly better than normal fermentation, and do not adhere to, mobility is better.
The Bacillus licheniformis liquid preparation obtained by the present embodiment and solid preparation carry out animal (weanling pig) test respectively, and test the present embodiment is to the growth promotion of animal (weanling pig) and the effect adjusting intestinal microflora.
Experimental technique: choose 21 age in days weanling pig 192, be divided into 3 treatment group, each treatment group 8 repetition, each repetition 8, three groups of basal diets of all feeding, do not add any material in blank group drinking-water, by 150mg/kg, (namely bacterium colony level is 1.5 × 10 in drinking-water for Liquid formulation trial group and solid preparation test group
9cfu/kg) the obtained Bacillus licheniformis liquid preparation of the present embodiment and solid preparation is added respectively, trial period 21d.Duration of test is observed each group of swinery and is searched for food and drinking-water situation, record feed consumption rate, measures the body weight of 21d after wean, calculates average daily gain, average daily ingestion amount and feed-weight ratio; Piglet ight soil situation is observed in timing every day, calculates diarrhea rate; Collect excrement sample during off-test, measure the quantity of wherein intestinal bacteria, lactobacillus and bifidus bacillus.Test experimental result is shown in
table 2and
table 3.
table 2bacillus licheniformis liquid preparation and the growth performance of solid preparation on weanling pig and the impact of diarrhea rate
| Group | Blank group | Liquid formulation trial group | Solid preparation test group |
| Average starting weight (kg) | 7.05 | 7.08 | 6.96 |
| Average end heavy (kg) | 11.01 | 11.95 | 12.11 |
| Average daily gain (g) | 189 b | 232 a | 245 a |
| Average daily ingestion amount (g) | 302 | 354 | 372 |
| Feed-weight ratio | 1.61 a | 1.53 b | 1.53 b |
| Diarrhea rate (%) | 7.91 a | 6.24 b | 6.38 b |
Note:
table 2middle data mark different letter representation significant difference (p<0.05).
table 2result show: add the average daily gain that the Bacillus licheniformis liquid preparation for livestock and fowl drinking water of the present embodiment and solid preparation all can significantly improve weanling pig, reduce feed-weight ratio, reduce diarrhea rate; And liquid preparation and solid preparation to the raising of Growth Performance of Weaning Piglets and the control of diarrhea rate without significant difference.
table 3bacillus licheniformis liquid preparation and solid preparation are on the impact of Intestinal Microflora of Weanling Piglets
| Group | Blank group | Liquid formulation trial group | Solid preparation test group |
| Intestinal bacteria (lgcfu/g) | 8.26 a | 7.75 b | 7.66 b |
| Lactobacillus (lgcfu/g) | 8.86 b | 9.77 a | 9.83 a |
| Bifidus bacillus (lgcfu/g) | 9.47 b | 10.68 a | 10.61 a |
Note:
table 3middle data mark different letter representation significant difference (p<0.05).
table 3result show: add the Bacillus licheniformis liquid preparation for livestock and fowl drinking water of the present embodiment and solid preparation all significantly can reduce weanling pig intestinal bacteria quantity, improve lactobacillus and bifidobacteria; And without significant difference between Liquid formulation trial group and solid preparation test group.
It can thus be appreciated that Bacillus licheniformis liquid preparation prepared by the preparation method of a kind of Bacillus licheniformis preparation for livestock and fowl drinking water of the present embodiment and solid preparation have Bacillus licheniformis the pulvis better water-soluble and mobility of producing compared with normal fermentation; And all can promote the growth of beneficial microorganism in weanling pig enteron aisle (lactobacillus and bifidus bacillus) and suppress the breeding of harmful microorganism (intestinal bacteria), improve the growth performance of weanling pig, and control diarrhea rate.
Claims (10)
1. for a preparation method for the Bacillus licheniformis preparation of livestock and fowl drinking water, it is characterized in that, comprise the preparation method of liquid preparation and solid preparation, wherein,
The preparation method of liquid preparation comprises the steps:
1a) getting appropriate Bacillus licheniformis is inoculated in the triangular flask including substratum I, cultivates, obtain first order seed in cultivation shaking table;
2a) get the first extractor, and drop into the medium ii of preparation, regulate pH to carry out boiling after 7.0, digested liquid is staticly settled, supernatant liquid is transferred to seed fermenter;
3a) by step 2a) seed fermenter in after supernatant liquid carries out high-temperature sterilization, drop into step 1a) first order seed, enlarged culturing obtains Bacillus licheniformis liquid;
4a) get the second extractor, and drop into the medium ii of preparation, regulate pH to carry out boiling after 7.0, digested liquid is staticly settled, supernatant liquid is transferred to fermentor tank;
5a) by step 4a) fermentor tank in after supernatant liquor carries out high-temperature sterilization, drop into step 3a) Bacillus licheniformis liquid, fermentation culture is until gemma transformation efficiency reaches after more than 95%, stop fermentation, qualification Bacillus licheniformis viable count, and to adjust pH be 3.5-4.0, obtain fermented liquid;
6a) according to detection level and target product content, determine Dilution ratio, to step 5a) fermented liquid in filling sterilized water, after 100 eye mesh screens filter, to be produced into Bacillus licheniformis level be 1.0 × 10
10the liquid preparation of cfu/g;
The preparation method of solid preparation comprises the steps:
1b) getting appropriate Bacillus licheniformis is inoculated in the triangular flask including substratum I, cultivates, obtain first order seed in cultivation shaking table;
2b) get the first extractor, and drop into the medium ii of preparation, regulate pH to carry out boiling after 7.0, digested liquid is staticly settled, supernatant liquid is transferred to seed fermenter;
3b) by step 2b) seed fermenter in after supernatant liquid carries out high-temperature sterilization, drop into step 1b) first order seed, enlarged culturing obtains Bacillus licheniformis liquid;
4b) get the second extractor, and drop into the medium ii of preparation, regulate pH to carry out boiling after 7.0, digested liquid is staticly settled, supernatant liquid is transferred to fermentor tank;
5b) by step 4b) fermentor tank in after supernatant liquor carries out high-temperature sterilization, drop into step 3b) Bacillus licheniformis liquid, fermentation culture is until gemma transformation efficiency reaches after more than 95%, stop fermentation, qualification Bacillus licheniformis viable count, and to adjust pH be 3.5-4.0, obtain fermented liquid;
6b) by step 5b) fermented liquid filter with 100 eye mesh screens after obtain clear liquid, according to detection limit and solid-to-liquid ratio, centrifugal and concentrated after dropping into the mixing of solubility auxiliary material, then obtaining Bacillus licheniformis level through spraying dry is 2.0 × 10
11the solid solubility preparation of cfu/g.
2. the preparation method of a kind of Bacillus licheniformis preparation for livestock and fowl drinking water according to claim 1, it is characterized in that: described step 1a) and step 1b) the Bacillus licheniformis of Bacillus licheniformis to be preserving number be CGMCCNo.9385, this Bacillus licheniformis liquid hold-up is 1.5 × 10
10cfu/g.
3. the preparation method of a kind of Bacillus licheniformis preparation for livestock and fowl drinking water according to claim 1, it is characterized in that: described step 1a) and step 1b) substratum I be beef extract-peptone Shake flask medium, pH for high-temperature sterilization is 7.0 and includes 5g/L extractum carnis, the water base substratum of 10g/L peptone and 5g/L sodium-chlor; In described cultivation shaking table, incubation time is 16-20h.
4. the preparation method of a kind of Bacillus licheniformis preparation for livestock and fowl drinking water according to claim 1, it is characterized in that: described step 2a) and step 2b) medium ii be fermention medium, the pH for high-temperature sterilization is 7.0 and includes the water base substratum of 5g/L swelling soya dreg, 12g/L Semen Maydis powder, 10g/L W-Gum, 5g/L calcium chloride, 10g/L yeast extract, 1g/L potassium primary phosphate, 0.05g/L manganous sulfate, 1.8g/L magnesium sulfate, 10g/L corn steep liquor, 20g/L wheat bran and 1g/L soya-bean oil.
5. the preparation method of a kind of Bacillus licheniformis preparation for livestock and fowl drinking water according to claim 1, it is characterized in that: described step 2a), step 4a), step 2b) and step 4b) conditions of cooking be temperature 100 DEG C, time 1-3h, sedimentation time is 3-5h.
6. the preparation method of a kind of Bacillus licheniformis preparation for livestock and fowl drinking water according to claim 1, it is characterized in that: described step 3a) and step 3b) in drop into first order seed time, seed fermenter temperature is 37 DEG C, and tank pressure is 0.01-0.02Mpa; Described enlarged culturing condition is temperature 37-39 DEG C, time 18-20h, stirs, air flow 10-12m
3/ h.
7. the preparation method of a kind of Bacillus licheniformis preparation for livestock and fowl drinking water according to claim 1, is characterized in that: described step 5a) and step 5b) in drop into Bacillus licheniformis liquid be 150L; When dropping into Bacillus licheniformis liquid, fermentation jar temperature is 37 DEG C, and tank pressure is 0.01-0.02Mpa; Described culture condition is temperature 37-39 DEG C, time 16-24h, stirs, air flow 800-1000m
3/ h.
8. the preparation method of a kind of Bacillus licheniformis preparation for livestock and fowl drinking water according to claim 1, is characterized in that: described step 1a) and step 1b) triangular flask content be 5L, in-built 2L substratum I; Described step 2a) and step 2b) the first extractor capacity be 500L, in-built 300L medium ii; Described step 4a) and step 4b) the second extractor capacity be 20T, in-built 15T medium ii.
9. the preparation method of a kind of Bacillus licheniformis preparation for livestock and fowl drinking water according to claim 1, is characterized in that: described step 6b) solubility auxiliary material is dextrin; The container of described centrifugal employing is disk centrifugal separator; Described concentrated condition is the 30-35% being concentrated into original volume; Described spraying dry is with intake air temperature 200-220 DEG C in spray-drying tower, and air outlet temperature 90-100 DEG C is carried out spraying dry.
10. the preparation method of a kind of Bacillus licheniformis preparation for livestock and fowl drinking water according to claim 1 or 3 or 4, is characterized in that: described high-temperature sterilization condition is temperature 121-128 DEG C, time 30-35min.
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