CN104611195A - High-concentration liquid multi-component raw material anaerobic bacterium-rich fermentation apparatus and technology thereof - Google Patents
High-concentration liquid multi-component raw material anaerobic bacterium-rich fermentation apparatus and technology thereof Download PDFInfo
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- CN104611195A CN104611195A CN201310539343.0A CN201310539343A CN104611195A CN 104611195 A CN104611195 A CN 104611195A CN 201310539343 A CN201310539343 A CN 201310539343A CN 104611195 A CN104611195 A CN 104611195A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/04—Bioreactors or fermenters specially adapted for specific uses for producing gas, e.g. biogas
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
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- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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Abstract
The invention discloses a high-concentration liquid multi-component raw material anaerobic bacterium-rich fermentation apparatus and a technology thereof, and belongs to the biotechnical field. The apparatus is composed of an anaerobic bacterium enrichment tank and an anaerobic reactor. The anaerobic bacterium enrichment tank is divided into a complete mixing zone, a strain enrichment zone and a settling zone from bottom to top, wherein the complete mixing zone is provided with a cyclone water distributor and a lateral stirrer, the strain enrichment zone is provided with a filler, and the settling zone is provided with a three-phase separator and a sludge discharging tube. The filler can effectively improve the concentration of flora as a microbial carrier; the lateral stirrer induces water flow disturbance, and allows excess microbes attached on the filler to be washed down and to be fully mixed with a fermentation raw material in order to promote the degradation of the fermentation raw material; the three-phase separator is used to separate biogas, a fermentation liquid and granular sludge; and the sludge discharging tube is used for conveying high concentration anaerobic bacteria to the anaerobic reactor. The anaerobic bacterium enrichment tank is constructed in the invention in order to improve the concentration and activity of the florae, widen the adaptability of the fermentation raw material, promote the degradation of the fermentation raw material and increase the volumetric organic load and the volumetric gas productivity.
Description
Technical field
Patent of the present invention belongs to biological technical field, relate to the rich anaerobic bacterial fermentation device of the liquid multicomponent raw material of a kind of high density and technique thereof, be particularly applicable in anaerobic digestion field, set about from key influence factors such as fermentation raw material, flora and toolings, realize the enrichment of anaerobic fermentation bacterial in high density liquid raw material condition, improve anaerobic digestion efficiency and volume yield rate.
Background technology
Along with the fast development of Chinese society economy, the organic waste such as agricultural organic waste, organic garbage of city is increasing, brings a series of problem such as environment and ecological aspect; Meanwhile, along with new countryside construction progress faster, the residential pattern in villages and small towns is progressively changed to hubbed mode by dispersion pattern, and the demand of life in the countryside energy increases gradually, increasing to the demand of high-grade energy.The how effectively increasing rubbish in process and comprehensive utilization city and villages and small towns and waste, realizes innoxious, minimizing, resource utilization, and the environment protection and tapping a new source of energy for China is of great immediate significance.
At present, China rural area and cities and towns organic waste are feces of livestock and poultry, agricultural crop straw, industrialized agriculture rubbish, changing food waste, domestic organic garbage etc. substantially, the common feature of these wastes is that organic content is high, anaerobically fermenting mode is adopted to carry out harmless treatment and recycling, not only effectively can administer the problem of environmental pollution that the unordered discharge of waste causes, reproducible clean energy can also be produced, alleviate energy-intensive situation.But current traditional marsh gas fermentation process volume yield rate is low, deficiency in economic performance, be mainly subject to the restriction of the unfavorable factors such as raw material is single, bacterial classification enrichment difficulty, technology and device do not mate.Therefore, for Diversity fermentation raw material, develop a kind of enrichment degree that can improve anaerobic bacteria flora, and then the efficient anaerobic fermentation technique improving whole reactor volume factor of created gase is very necessary.
Summary of the invention
The object of the invention is the problems such as liquid material concentration for current anaerobic ferment process process is lower, raw material is single, volume yield rate is low, by building anerobe enrichment tank, improve flora concentration and activity, added the anerobe of high reactivity, high density to anaerobic reactor by anerobe enrichment tank, widen the adaptability of fermentation raw material, promote the degraded of fermentation raw material, improve volume organic loading and the volume yield rate of anaerobic reactor.
As follows for reaching the technical scheme that this purpose takes: the rich anaerobic bacterial fermentation device of the liquid multicomponent raw material of a kind of high density and technique thereof are provided.
The rich anaerobic bacterial fermentation device of the liquid multicomponent raw material of high density comprises anerobe enrichment tank and anaerobic reactor.Anerobe enrichment tank is divided into complete mixing zone, bacterial classification enrichment region and three, settling region district, and wherein mixing zone is provided with cyclone water distributor, lateral stirrer completely, and bacterial classification enrichment region is provided with filler, and settling region is provided with triphase separator, shore pipe; The volume ratio of complete mixing zone, bacterial classification enrichment region and settling region is 1:2:1.
Described complete mixing zone is positioned at anerobe enrichment tank bottom, and from lower from being provided with cyclone water distributor, lateral stirrer, high gravity fermentation raw material evenly upwards flows after entering cyclone water distributor by feed-pipe; Lateral stirrer causes turbulent water, washing away, and fully being mixed with fermentation raw material by these living microorganisms further, promoting that it is degraded by filler adhering to too much microorganism.
Described bacterial classification enrichment region is positioned in the middle part of anerobe enrichment tank, is provided with the filler in standing tank, and as the carrier of microorganic adhesion, active anaerobic flora is attached thereto in a large number, effectively can improve the concentration of flora, reach the object of enrichment anerobe.
Described settling region is positioned at anerobe enrichment tank top, be provided with shore pipe and triphase separator from bottom to top, shore pipe is used for the anaerobic species of high density activity in tank to be delivered in anaerobic reactor, and triphase separator is for separating of biogas, fermented feed liquid and granule sludge.
Described anerobe enrichment tank is connected by shore pipe with anaerobic reactor, and the volume ratio of anerobe enrichment tank and anaerobic reactor is 1:10 ~ 100.
The rich anaerobic bacterial fermentation technique of the liquid multicomponent raw material of high density comprises the following steps:
The high density mixed fermentation raw material mixed is added in anerobe enrichment tank, and inoculate the active sludge that volume is about fermentation raw material 1/3, setting mixing speed is 10 ~ 20r/min, the environment utilizing anaerobic bacteria flora Growth and reproduction is built in anerobe enrichment tank, controlling in tank is middle temperature (35 ~ 38 DEG C), feed concentration is 8% ~ 12%, and dissolved oxygen is 0 ~ 0.01mg/L, pH is 6.8 ~ 7.5.
Detect every day and record the gas production rate of anerobe enrichment tank, observe the time that its aerogenesis peak occurs, when gas production rate continuous decrease, every day adds appropriate fermentation raw material in tank, cultivates a few days, until anerobe enrichment tank aerogenesis is stablized.
In anaerobic reactor, add the high density multicomponent raw material that feed concentration is 8% ~ 12%, inoculation volume is about the active sludge of fermentation raw material 1/3, and setting mixing speed is 20 ~ 30r/min, and middle temperature (35 ~ 38 DEG C), cultivates 2 to 3 days.
Every day releases appropriate residues from anaerobic reactor, add appropriate fermentation raw material (being about 9/10 ~ 7/8 of discharging volume) again, and from anerobe enrichment tank shore pipe, in anaerobic reactor, add a certain amount of high density anerobe liquid (being about 1/10 ~ 1/8 of discharging volume).Meanwhile, in anerobe enrichment tank, the fermentation raw material identical with the material liquid volume that shore pipe is discharged is added.
The beneficial effect that the present invention compared with prior art has is: in anerobe enrichment tank, 1) build the growth and breeding environment being conducive to biogas fermentation anaerobic bacteria flora, realize the selectivity optimization of anaerobic species, by filler and triphase separator, activity and the quantity that effectively can improve biogas fermentation anaerobic species are set, microorganism too much on filler can be washed away by agitator, make it mix with fermentation raw material, improve degradation rate; 2) anaerobic bacteria flora of anerobe enrichment tank adopts Diversity raw material to cultivate, its raw material adaptive faculty is stronger, being added to after in anaerobic reactor, the degraded demand of anaerobic reactor different material can be adapted to, widening the fermentation raw material type of anaerobic reactor; 3) by adding high density anaerobic species from anerobe enrichment tank in anaerobic reactor, flora concentration in reactor can be improved, effectively promoting organic degraded, improve the degradation rate of fermentation raw material and the volume yield rate of reactor; 4) anerobe of high density can improve the volume organic loading rate of anaerobic reactor, adapts to the fermentation raw material of greater concn, improves processing efficiency.
Accompanying drawing illustrates:
Fig. 1 is the rich anaerobic bacterial fermentation device of the liquid multicomponent raw material of high density and system schematic thereof.
Embodiment:
As shown in Figure 1:
1 is anerobe enrichment tank, and 2 is anaerobic reactor, and 3 is feed-pipe, and 4 is cyclone water distributor, and 5 is lateral stirrer, and 6 is filler, and 7 is triphase separator, and 8 is shore pipe, and 9 is stopple coupon, and 10 is vertical agitator.
Be described below in conjunction with accompanying drawing 1 pair of embodiments of the invention:
As shown in Figure 1, the rich anaerobic bacterial fermentation device of the liquid multicomponent raw material of high density comprises anerobe enrichment tank 1 and anaerobic reactor 2.Anerobe enrichment tank 1 is divided into complete mixing zone, bacterial classification enrichment region and three, settling region district, and wherein mixing zone is provided with cyclone water distributor 4, lateral stirrer 5 completely, and bacterial classification enrichment region is provided with filler 6, and settling region is provided with triphase separator 7, shore pipe 8; The volume ratio of complete mixing zone, bacterial classification enrichment region and settling region is 1:2:1.
Described complete mixing zone is positioned at anerobe enrichment tank 1 bottom, and from lower from being provided with cyclone water distributor 4, lateral stirrer 5, high gravity fermentation raw material evenly upwards flows after entering cyclone water distributor 4 by feed-pipe 3; Lateral stirrer 5 causes turbulent water, washing away, and fully being mixed with fermentation raw material by these living microorganisms further, promoting that it is degraded by filler 6 adhering to too much microorganism.
Described bacterial classification enrichment region is positioned in the middle part of anerobe enrichment tank 1, is provided with the filler 6 in standing tank, and as the carrier of microorganic adhesion, active anaerobic flora is attached thereto in a large number, effectively can improve the concentration of flora, reach the object of enrichment anerobe.
Described settling region is positioned at anerobe enrichment tank 1 top, be provided with shore pipe 8 and triphase separator 7 from bottom to top, shore pipe 8 is for being delivered in anaerobic reactor 2 by the anaerobic species of high density activity in tank, and triphase separator 7 is for separating of biogas, fermented feed liquid and granule sludge.
Described anerobe enrichment tank 1 is connected by shore pipe 8 with anaerobic reactor 2, and the volume ratio of anerobe enrichment tank 1 and anaerobic reactor 2 is 1:10 ~ 100.
The rich anaerobic bacterial fermentation technique of the liquid multicomponent raw material of high density comprises the following steps:
The high density mixed fermentation raw material mixed is added in anerobe enrichment tank 1, and inoculate the active sludge that volume is about fermentation raw material 1/3, setting mixing speed is 10 ~ 20r/min, the environment utilizing anaerobic bacteria flora Growth and reproduction is built in anerobe enrichment tank, controlling in tank is middle temperature (35 ~ 38 DEG C), feed concentration is 8% ~ 12%, and dissolved oxygen is 0 ~ 0.01mg/L, pH is 6.8 ~ 7.5.
Detect every day and record the gas production rate of anerobe enrichment tank 1, observe the time that its aerogenesis peak occurs, when gas production rate continuous decrease, every day adds appropriate fermentation raw material in tank, cultivates a few days, until anerobe enrichment tank 1 aerogenesis is stablized.
In anaerobic reactor 2, add the high density multicomponent raw material that feed concentration is 8% ~ 12%, inoculation volume is about the active sludge of fermentation raw material 1/3, and setting mixing speed is 20 ~ 30r/min, and middle temperature (35 ~ 38 DEG C), cultivates 2 to 3 days.
Every day releases appropriate residues from anaerobic reactor 2, add appropriate fermentation raw material (being about 9/10 ~ 7/8 of discharging volume) again, and from the shore pipe 8 of anerobe enrichment tank 1, in anaerobic reactor 2, add a certain amount of high density anerobe liquid (being about 1/10 ~ 1/8 of discharging volume).Meanwhile, in anerobe enrichment tank 1, the fermentation raw material identical with the material liquid volume that shore pipe 8 is discharged is added.
Finally it should be noted that, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although with reference to preferred embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that, can modify to technical scheme of the present invention or equivalent replacement, and not departing from the spirit and scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.
Claims (6)
1. the rich anaerobic bacterial fermentation device of the liquid multicomponent raw material of high density, is characterized in that comprising anerobe enrichment tank (1) and anaerobic reactor (2).Anerobe enrichment tank (1) is divided into complete mixing zone, bacterial classification enrichment region and three, settling region district, wherein mixing zone is provided with cyclone water distributor (4), lateral stirrer (5) completely, bacterial classification enrichment region is provided with filler (6), and settling region is provided with triphase separator (7), shore pipe (8); The volume ratio of complete mixing zone, bacterial classification enrichment region and settling region is 1:2:1.
2. the rich anaerobic bacterial fermentation device of the liquid multicomponent raw material of a kind of high density according to claim 1, it is characterized in that described complete mixing zone is positioned at anerobe enrichment tank (1) bottom, from lower from being provided with cyclone water distributor (4), lateral stirrer (5), high gravity fermentation raw material evenly upwards flows after entering cyclone water distributor (4) by feed-pipe (3); Lateral stirrer (5) causes turbulent water, is washed away by the too much microorganism of the upper attachment of filler (6), and is fully mixed with fermentation raw material by these living microorganisms further.
3. the rich anaerobic bacterial fermentation device of the liquid multicomponent raw material of a kind of high density according to claim 1, it is characterized in that described bacterial classification enrichment region is positioned at anerobe enrichment tank (1) middle part, be provided with the filler (6) in standing tank, as the carrier of microorganic adhesion.
4. the rich anaerobic bacterial fermentation device of the liquid multicomponent raw material of a kind of high density according to claim 1, it is characterized in that described settling region is positioned at anerobe enrichment tank (1) top, be provided with shore pipe (8) and triphase separator (7) from bottom to top, shore pipe (8) is for being delivered in anaerobic reactor (2) by the anaerobic species of high density activity in tank, and triphase separator (7) is for separating of biogas, fermented feed liquid and granule sludge.
5. the rich anaerobic bacterial fermentation device of the liquid multicomponent raw material of a kind of high density according to claim 1, it is characterized in that described anerobe enrichment tank (1) is connected by shore pipe (8) with anaerobic reactor (2), the volume ratio of anerobe enrichment tank (1) and anaerobic reactor (2) is 1:10 ~ 100.
6. use the rich anaerobic bacterial fermentation technique of the liquid multicomponent raw material of the high density of device according to claim 1, it is characterized in that comprising the following steps:
The high density mixed fermentation raw material mixed is added in anerobe enrichment tank (1), and inoculate the active sludge that volume is about fermentation raw material 1/3, setting mixing speed is 10 ~ 20r/min, the environment utilizing anaerobic bacteria flora Growth and reproduction is built in anerobe enrichment tank, controlling in tank is middle temperature (35 ~ 38 DEG C), feed concentration is 8% ~ 12%, and dissolved oxygen is 0 ~ 0.01mg/L, pH is 6.8 ~ 7.5.
Detect every day and record the gas production rate of anerobe enrichment tank (1), observe the time that its aerogenesis peak occurs, when gas production rate continuous decrease, every day adds appropriate fermentation raw material in tank, cultivates a few days, until anerobe enrichment tank 1 aerogenesis is stablized.
The high density multicomponent raw material that feed concentration is 8% ~ 12% is added in anaerobic reactor (2), inoculation volume is about the active sludge of fermentation raw material 1/3, setting mixing speed is 20 ~ 30r/min, and middle temperature (35 ~ 38 DEG C), cultivates 2 to 3 days.
Every day releases appropriate residues from anaerobic reactor (2), add appropriate fermentation raw material (being about 9/10 ~ 7/8 of discharging volume) again, and in anaerobic reactor (2), add a certain amount of high density anerobe liquid (being about discharging volume 1/10 ~ 1/8) from the shore pipe (8) of anerobe enrichment tank (1).Meanwhile, in anerobe enrichment tank (1), the fermentation raw material identical with the material liquid volume that shore pipe (8) is discharged is added.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110982675A (en) * | 2019-12-23 | 2020-04-10 | 徐州生物工程职业技术学院 | Microorganism enrichment breeding device |
| CN115321755A (en) * | 2022-08-18 | 2022-11-11 | 南京工业大学 | Sewage treatment system and method for manganese oxidizing bacteria reinforced biological filter |
-
2013
- 2013-11-05 CN CN201310539343.0A patent/CN104611195A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110982675A (en) * | 2019-12-23 | 2020-04-10 | 徐州生物工程职业技术学院 | Microorganism enrichment breeding device |
| CN110982675B (en) * | 2019-12-23 | 2022-12-20 | 徐州生物工程职业技术学院 | Microorganism enrichment breeding device |
| CN115321755A (en) * | 2022-08-18 | 2022-11-11 | 南京工业大学 | Sewage treatment system and method for manganese oxidizing bacteria reinforced biological filter |
| CN115321755B (en) * | 2022-08-18 | 2024-04-16 | 南京工业大学 | Sewage treatment system and method for manganese oxidizing bacteria reinforced biological filter |
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Application publication date: 20150513 |