CN104609555A - Sequencing-batch intermittent activated sludge reactor and method for enriching nitrifying bacteria and denitrifying bacteria by using reactor - Google Patents
Sequencing-batch intermittent activated sludge reactor and method for enriching nitrifying bacteria and denitrifying bacteria by using reactor Download PDFInfo
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Abstract
The invention provides a sequencing-batch intermittent activated sludge reactor. The reactor comprises a reactor body, a magnetic stirrer, a pH value measuring and indicating device, a DO (dissolved oxygen) measuring and indicating device, a blast aeration machine, a nitrogen supply pipeline, a water inlet pump, a water outlet pump and a temperature measuring and indicating device, wherein the top of the reactor body is sealed with a plastic pad; the magnetic stirrer is arranged at the bottom of the reactor body; the pH value measuring and indicating device, the DO measuring and indicating device and the temperature measuring and indicating device are used for measuring the pH value, the DO value and the temperature value of a liquid in the reactor body respectively; the water inlet pump and the water outlet pump are used for pumping water into or out of the reactor body respectively; the blast aeration machine and the nitrogen supply pipeline are used for supplying air and nitrogen to the reactor body respectively. The invention further provides a method for enriching nitrifying bacteria and denitrifying bacteria by using the reactor. According to the reactor and the method, the technological process and the structure are simple, operations are convenient, the harmful gas reduction effect is good, the practicability is high, and the prospect is broad.
Description
Technical field
The invention belongs to sewage disposal control field, particularly relate to a kind of sequence batch (interval activated sludge reaction device and utilize the method for its enrichment nitrifier and denitrifying bacteria.
Background technology
N
2o is a kind of important greenhouse gases in air, and its Greenhouse effect are about CO
2310 times.In addition, N
2o is the major cause causing depletion of the ozone layer at present, and its existence in an atmosphere can also form acid rain.There are some researches show, the biological denitrification process of sewage disposal is N
2one of main source of O.Therefore, N in bio-denitrifying sewage process is studied
2the mechanism of production of O, proposes effective and feasible N
2o minimizing control strategy has positive effect.
In bio-denitrifying sewage process, the removal of nitrogen is reacted mainly through the nitrification and denitrification of microorganism.Research proves, all can produce N in nitrifying process and denitrification process
2o and NO and NO
2.Give full play to the effect of nitrifier and denitrifying bacteria thus realize N
2o and NO and NO
2minimizing, the matter of utmost importance that solve carries out enrichment culture to nitrifier and denitrifying bacteria, and adjust operation condition is to reaching optimal operating condition.
It is simple that SBR technique has technical process, treatment effect is stablized, run the advantages such as easy, its ultimate principle is that the operation condition of ammonia nitrogen process under aerobic condition in sewage is converted into nitrate nitrogen and nitrite nitrogen by nitrification, under the condition of anoxic, change N into by the approach of denitrification and short-cut denitrification
2.
Tradition N
2o and NO and NO
2minimizing control strategy great majority are in the operational conditions exploring technique thus improve nitrated and denitrification process, but these measure of control can cause certain disadvantageous effect to the nitrogen removal performance of system while realizing obnoxious flavour minimizing.And to by adjustment microbiological paper method and on this basis Reasonable Regulation And Control operation condition thus ensure efficient denitrification basis on realize N
2o and NO and NO
2the minimizing research of gas is very few.
Summary of the invention
The object of the invention is to for the deficiencies in the prior art, provide on a kind of basis ensureing efficient denitrification and realize N
2o and NO and NO
2the sequence batch (interval activated sludge reaction device of gas minimizing and utilize the method for its enrichment nitrifier and denitrifying bacteria.
The present invention for solving the problems of the technologies described above adopted scheme is:
A kind of sequence batch (interval activated sludge reaction device, it comprises reactor, it is characterized in that, the top of described reactor adopts plastic pad sealing, and described sequence batch (interval activated sludge reaction device also comprises magnetic stirring apparatus, pH value is measured telltale, dissolved oxygen sensing telltale, blast aeration machine, nitrogen supply (NS) pipeline, intake pump, gone out water pump and temperature survey telltale; Described magnetic stirring apparatus is arranged at the bottom of described reactor, described pH value is measured telltale, dissolved oxygen sensing telltale and temperature survey telltale and is respectively used to measure the pH value of liquid in described reactor, dissolved oxygen value and temperature value, described intake pump and go out water pump and be respectively used to adding water of described reactor or draw water, described blast aeration machine and nitrogen supply (NS) pipeline are respectively used to described reactor supply air and nitrogen.
In such scheme, described sequence batch (interval activated sludge reaction device also comprises into water bucket and goes out bucket, and described intake pump is positioned in described water inlet bucket, described in go out water pump be positioned over described in go out in bucket.
In such scheme, described sequence batch (interval activated sludge reaction device also comprises the liquid level relay for controlling described reactor liquid level.
Utilize a method for sequence batch (interval activated sludge reaction device enrichment nitrifier and denitrifying bacteria, it comprises the steps:
1) seed sludge after domestication is transferred to and be equipped with in the reactor of waste water in sequence batch (interval activated sludge reaction device;
2) pattern enrichment nitrifier and the denitrifying bacteria of aerobic and anoxic alternate run is taked;
3) further optimizing operation operating mode under nitrifier and denitrifying bacteria have highly active condition;
4) basis of steady running after reactor optimization changes loading factor C/N ratio and DO value, to NO, NO
2, N
2escaped quantity and the total nitrogen reduction of O are measured.
In such scheme, described step 2) in aerobic section dissolved oxygen control at 2.0 ~ 2.6mg/L, anoxic section dissolved oxygen controls at 0.1 ~ 0.7mg/L.
In such scheme, described step 2) in the aerobic section time be 6h, the anoxic section time is 5h, sedimentation time 0.5h, and the water-in and water-out time is 0.25h.
In such scheme, described waste water ph is 7 ~ 8, and water temperature is 25 ~ 35 DEG C.
In such scheme, described step 1) in adopt sodium acetate and ammonium chloride as the Carbon and nitrogen sources of mud after domestication, C/N is than being 3-8.
In such scheme, described step 2) in aerobic section dissolved oxygen control at 2.0 ~ 2.6mg/L, anoxic section dissolved oxygen controls at 0.3mg/L, described step 1) in C/N ratio be 8.
For ensureing the running environment that reactor is good, around reactor, use thermostatical circulating water to maintain the temperature of reaction solution.Waste water used is the strong chemical production wastewater (water quality table 1) of Practical Project Yichang China, and the mud inoculated is oxygen-starved area mud (mud parameter list 2) during this enterprise's sewage treatment project operational excellence.
The strong waste water quality parameter list of table 1 China
| Parameter | COD cr | TN | C | NO 3 --N | NO 2 --N | pH |
| Raw water quality | 500～800 | 300～600 | 250～500 | 50～100 | <10 | 7.20～8.62 |
Table 2 oxygen-starved area mud parameter list
| Color | Form | MLSS(mg/L) | MLVSS(mg/L) | SVI(ml/g) | SV 30(％) |
| Deep yellow | Cotton-shaped, nothing fragmentation | 4251 | 4067 | 63.5 | 27 |
Beneficial effect of the present invention is: technical process is simple, and structure is simple, and easy to operate, good to obnoxious flavour reduction, practical, prospect is extensive.
Accompanying drawing explanation
Fig. 1 is the structural representation of the sequence batch (interval activated sludge reaction device described in the embodiment of the present invention.
Fig. 2 is stable rear nitrifier electromicroscopic photograph.
Fig. 3 is stable rear denitrifying bacteria electromicroscopic photograph.
In figure: 1-synthetic glass cylindrical reactor; 2-magnetic stirring apparatus; 3-pH value measures telltale; 4-DO measures telltale; 5-plastic pad; 6-temperature survey telltale; 7-gas collection mouth; 8-high pure nitrogen supply line; 9-spinner-type flowmeter; 10-blast aeration machine; 11-intake pump; 12-goes out water pump; 13-liquid level relay; 14-is intake bucket; 15-goes out bucket.
Embodiment
Below in conjunction with drawings and Examples, the present invention will be described further, but content of the present invention is not only confined to the following examples.
Embodiment 1
The present invention runs aerobic/anoxic pattern in homemade sequence batch (interval activated sludge reaction device, sets up the nitrifier of high selectivity and the culture environment of denitrifying bacteria, thus realizes the enrichment of nitrifier and denitrifying bacteria in sbr reactor device.And carry out searching system optimal operating condition by adjustment loading factor C/N and DO value thus realize obnoxious flavour minimizing in denitrification process.
As shown in Figure 1, its a kind of sequence batch (interval activated sludge reaction device (being called for short SBR) for realizing obnoxious flavour minimizing in sewage water denitrification process provided for the embodiment of the present invention, it comprises synthetic glass cylindrical reactor 1, the top of described reactor adopts plastic pad 5 to seal, and described sequence batch (interval activated sludge reaction device also comprises magnetic stirring apparatus 2, pH value is measured telltale 3, dissolved oxygen sensing telltale 4, blast aeration machine 10, nitrogen supply (NS) pipeline 8, intake pump 11, gone out water pump 12 and temperature survey telltale 6.Magnetic stirring apparatus 2 is arranged at the bottom of described reactor 1, and described pH value is measured telltale 3, dissolved oxygen sensing telltale 4 and temperature survey telltale 6 and is respectively used to measure the pH value of liquid in described reactor 1, dissolved oxygen value and temperature value.Intake pump 11 and go out water pump 12 and be respectively used to adding water or drawing water of described reactor 1, described blast aeration machine 10 and nitrogen supply (NS) pipeline 8 are respectively used to supply air and nitrogen to described reactor 1.Blast aeration machine 10 regulates aeration rate by spinner-type flowmeter 9.The useful volume of the reactor 1 in the present embodiment is 2L.
The present embodiment also provides and utilizes sequence batch (interval activated sludge reaction device enrichment nitrifier and the method for denitrifying bacteria, and adopt sodium acetate and ammonium chloride as the Carbon and nitrogen sources of mud after domestication, C/N ratio is 3.Water inlet NH
4 +-N is 50mg/L; COD is 150mg/L; Aerobic section DO is 2.0 ~ 2.6mg/L; PH is 7.0 ~ 7.5.The sbr reactor device aerobic section time is 6h, and the anoxic section time is 5h, sedimentation time 0.5h, and the water-in and water-out time is 0.25h, and water temperature is 28 ~ 30 DEG C.NO, NO
2, N
2the maximum escaped quantity of O is respectively 0.827mg, 1.209mg, 4.207mg, and three kinds of total escaped quantities of gas are 6.243mg, N
2the escaped quantity of O account for into water TN ratio be 4.207%; The reduction of total nitrogen is 53.79mg, and oxygen-starved area denitrification percent is 43.55%, and total denitrification percent is 53.79%.
Embodiment 2
The sequence batch (interval activated sludge reaction device of the present embodiment is identical with embodiment 1, difference is to utilize the method for sequence batch (interval activated sludge reaction device enrichment nitrifier and denitrifying bacteria be: adopt sodium acetate and ammonium chloride as the Carbon and nitrogen sources of mud after domestication, C/N ratio is 4.Water inlet NH
4 +-N is 50mg/L; COD is 200mg/L; Aerobic section DO is 2.0 ~ 2.6mg/L; PH is 7.0 ~ 7.5.The sbr reactor device aerobic section time is 6h, and the anoxic section time is 5h, sedimentation time 0.5h, and the water-in and water-out time is 0.25h, and water temperature is 28 ~ 30 DEG C.NO, NO
2and N
2the maximum escaped quantity of O is respectively 0.542mg, 0.885mg and 2.808mg, and three kinds of total escaped quantities of gas are 4.235mg, N
2the escaped quantity of O account for into water TN ratio be 2.808%; The reduction of total nitrogen is 70.01mg, and oxygen-starved area denitrification percent is 58.24%, and total denitrification percent is 70.01%.
Embodiment 3
The sequence batch (interval activated sludge reaction device of the present embodiment is identical with embodiment 1, difference is to utilize the method for sequence batch (interval activated sludge reaction device enrichment nitrifier and denitrifying bacteria be: adopt sodium acetate and ammonium chloride as the Carbon and nitrogen sources of mud after domestication, C/N ratio is 6.Water inlet NH
4 +-N is 50mg/L; COD is 300mg/L; Aerobic section DO is 2.0 ~ 2.6mg/L; PH is 7.0 ~ 7.5.The sbr reactor device aerobic section time is 6h, and the anoxic section time is 5h, sedimentation time 0.5h, and the water-in and water-out time is 0.25h, and water temperature is 28 ~ 30 DEG C.NO, NO
2and N
2the maximum escaped quantity of O is respectively 0.550mg, 0.895mg and 2.565mg, and three kinds of total escaped quantities of gas are 4.010mg, N
2the escaped quantity of O account for into water TN ratio be 2.565%; The reduction of total nitrogen is 73.38mg, and oxygen-starved area denitrification percent is 61.07%, and total denitrification percent is 73.38%.
Embodiment 4
The sequence batch (interval activated sludge reaction device of the present embodiment is identical with embodiment 1, difference is to utilize the method for sequence batch (interval activated sludge reaction device enrichment nitrifier and denitrifying bacteria be: adopt sodium acetate and ammonium chloride as the Carbon and nitrogen sources of mud after domestication, C/N ratio is 8.Water inlet NH
4 +-N is 50mg/L; COD is 400mg/L; Aerobic section DO is 2.0 ~ 2.6mg/L; PH is 7.0 ~ 7.5.The sbr reactor device aerobic section time is 6h, and the anoxic section time is 5h, sedimentation time 0.5h, and the water-in and water-out time is 0.25h, and water temperature is 28 ~ 30 DEG C.NO, NO
2and N
2the maximum escaped quantity of O is respectively 0.547mg, 0.887mg and 2.457mg, and three kinds of total escaped quantities of gas are 3.891mg, N
2the escaped quantity of O account for into water TN ratio be 2.457%; The reduction of total nitrogen is 74.28mg, and oxygen-starved area denitrification percent is 62.74%, and total denitrification percent is 74.28%.
Embodiment 5
The sequence batch (interval activated sludge reaction device of the present embodiment is identical with embodiment 1, difference is to utilize the method for sequence batch (interval activated sludge reaction device enrichment nitrifier and denitrifying bacteria be: adopt sodium acetate and ammonium chloride as the Carbon and nitrogen sources of mud after domestication, C/N ratio is 8, makes aerobic section DO be 2.0 ~ 2.6mg/L; Anoxic section DO value is 0.7mg/L.Water inlet NH
4 +-N is 50mg/L; COD is 200mg/L; PH is 7.0 ~ 7.5.The sbr reactor device aerobic section time is 6h, and the anoxic section time is 5h, sedimentation time 0.5h, and the water-in and water-out time is 0.25h, and water temperature is 28 ~ 30 DEG C.NO, NO
2and N
2total escaped quantity of O is respectively 0.776mg, 1.214mg and 3.294mg, and three kinds of total escaped quantities of gas are 5.284mg, N
2the escaped quantity of O account for into water TN ratio be 3.294%; The reduction of total nitrogen is 47.12mg, and oxygen-starved area denitrification percent is 38.98%, and total denitrification percent is 47.12%.
Embodiment 6
The sequence batch (interval activated sludge reaction device of the present embodiment is identical with embodiment 1, difference is to utilize the method for sequence batch (interval activated sludge reaction device enrichment nitrifier and denitrifying bacteria be: adopt sodium acetate and ammonium chloride as the Carbon and nitrogen sources of mud after domestication, C/N ratio is 8, makes aerobic section DO be 2.0 ~ 2.6mg/L; Anoxic section DO value is 0.5mg/L.Water inlet NH
4 +-N is 50mg/L; COD is 200mg/L; PH is 7.0 ~ 7.5.The sbr reactor device aerobic section time is 6h, and the anoxic section time is 5h, sedimentation time 0.5h, and the water-in and water-out time is 0.25h, and water temperature is 28 ~ 30 DEG C.NO, NO
2and N
2total escaped quantity of O is respectively 0.578mg, 0.849mg and 2.347mg, and three kinds of total escaped quantities of gas are 3.774mg, N
2the escaped quantity of O account for into water TN ratio be 2.347%; The reduction of total nitrogen is 67.30mg, and oxygen-starved area denitrification percent is 60.80%, and total denitrification percent is 67.30%.
Embodiment 7
The sequence batch (interval activated sludge reaction device of the present embodiment is identical with embodiment 1, difference is to utilize the method for sequence batch (interval activated sludge reaction device enrichment nitrifier and denitrifying bacteria be: adopt sodium acetate and ammonium chloride as the Carbon and nitrogen sources of mud after domestication, C/N ratio is 8, makes aerobic section DO be 2.0 ~ 2.6mg/L; Anoxic section DO value is 0.3mg/L.Water inlet NH
4 +-N is 50mg/L; COD is 200mg/L; PH is 7.0 ~ 7.5.The sbr reactor device aerobic section time is 6h, and the anoxic section time is 5h, sedimentation time 0.5h, and the water-in and water-out time is 0.25h, and water temperature is 28 ~ 30 DEG C.NO, NO
2and N
2total escaped quantity of O is respectively 0.417mg, 0.619mg and 1.240mg, and three kinds of total escaped quantities of gas are 2.276mg/L, N
2the escaped quantity of O account for into water TN ratio be 1.240%; The reduction of total nitrogen is 72.70mg, and oxygen-starved area denitrification percent is 70.49%, and total denitrification percent is 72.70%.
Embodiment 8
The sequence batch (interval activated sludge reaction device of the present embodiment is identical with embodiment 1, difference is to utilize the method for sequence batch (interval activated sludge reaction device enrichment nitrifier and denitrifying bacteria be: adopt sodium acetate and ammonium chloride as the Carbon and nitrogen sources of mud after domestication, C/N ratio is 8, makes aerobic section DO be 2.0 ~ 2.6mg/L; Anoxic section DO value is 0.1mg/L.Water inlet NH
4 +-N is 50mg/L; COD is 200mg/L; PH is 7.0 ~ 7.5.The sbr reactor device aerobic section time is 6h, and the anoxic section time is 5h, sedimentation time 0.5h, and the water-in and water-out time is 0.25h, and water temperature is 28 ~ 30 DEG C.NO, NO
2and N
2total escaped quantity of O is respectively 0.540mg, 0.801mg and 1.185mg, and three kinds of total escaped quantities of gas are 2.526mg/L, N
2the escaped quantity of O account for into water TN ratio be 1.185%; The reduction of total nitrogen is 36.90mg, and oxygen-starved area denitrification percent is 33.03%, and total denitrification percent is 36.90%.
Claims (9)
1. a sequence batch (interval activated sludge reaction device, it comprises reactor, it is characterized in that, the top of described reactor adopts plastic pad sealing, and described sequence batch (interval activated sludge reaction device also comprises magnetic stirring apparatus, pH value is measured telltale, dissolved oxygen sensing telltale, blast aeration machine, nitrogen supply (NS) pipeline, intake pump, gone out water pump and temperature survey telltale; Described magnetic stirring apparatus is arranged at the bottom of described reactor, described pH value is measured telltale, dissolved oxygen sensing telltale and temperature survey telltale and is respectively used to measure the pH value of liquid in described reactor, dissolved oxygen value and temperature value, described intake pump and go out water pump and be respectively used to adding water of described reactor or draw water, described blast aeration machine and nitrogen supply (NS) pipeline are respectively used to described reactor supply air and nitrogen.
2. sequence batch (interval activated sludge reaction device as claimed in claim 1, it is characterized in that, described sequence batch (interval activated sludge reaction device also comprises into water bucket and goes out bucket, and described intake pump is positioned in described water inlet bucket, described in go out water pump be positioned over described in go out in bucket.
3. sequence batch (interval activated sludge reaction device as claimed in claim 1, is characterized in that, described sequence batch (interval activated sludge reaction device also comprises the liquid level relay for controlling described reactor liquid level.
4. utilize a method for sequence batch (interval activated sludge reaction device enrichment nitrifier and denitrifying bacteria, it is characterized in that, it comprises the steps:
1) seed sludge after domestication is transferred to and be equipped with in the reactor of waste water in sequence batch (interval activated sludge reaction device;
2) pattern enrichment nitrifier and the denitrifying bacteria of aerobic and anoxic alternate run is taked;
3) further optimizing operation operating mode under nitrifier and denitrifying bacteria have highly active condition;
4) basis of steady running after reactor optimization changes loading factor C/N ratio and DO value, to NO, NO
2, N
2escaped quantity and the total nitrogen reduction of O are measured.
5. method as claimed in claim 4, is characterized in that, described step 2) in aerobic section dissolved oxygen control at 2.0 ~ 2.6mg/L, anoxic section dissolved oxygen controls at 0.1 ~ 0.7mg/L.
6. method as claimed in claim 4, is characterized in that, described step 2) in the aerobic section time be 6h, the anoxic section time is 5h, sedimentation time 0.5h, and the water-in and water-out time is 0.25h.
7. method as claimed in claim 4, it is characterized in that, described waste water ph is 7 ~ 8, and water temperature is 25 ~ 35 DEG C.
8. method as claimed in claim 4, is characterized in that, described step 1) in adopt sodium acetate and ammonium chloride as the Carbon and nitrogen sources of mud after domestication, C/N is than being 3-8.
9. method as claimed in claim 4, is characterized in that, described step 2) in aerobic section dissolved oxygen control at 2.0 ~ 2.6mg/L, anoxic section dissolved oxygen controls at 0.3mg/L, described step 1) in C/N ratio be 8.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115487666A (en) * | 2022-08-26 | 2022-12-20 | 福州大学 | High-efficiency conversion of NO in flue gas into N 2 Method of O |
-
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Non-Patent Citations (2)
| Title |
|---|
| 贾文林: ""同步硝化反硝化过程中N2O释放特征及其机理研究"", 《中国博士学位论文全文数据库 工程科技I辑》 * |
| 龚云华等: "废水处理同时硝化/反硝化脱氮技术现状与展望", 《能源环境保护》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115487666A (en) * | 2022-08-26 | 2022-12-20 | 福州大学 | High-efficiency conversion of NO in flue gas into N 2 Method of O |
| CN115487666B (en) * | 2022-08-26 | 2023-09-12 | 福州大学 | Efficient conversion of NO in flue gas to N 2 O method |
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