CN104609555B - A kind of batch-type interval activated sludge reaction device and the method for utilizing its enrichment nitrifier and denitrifying bacterium - Google Patents
A kind of batch-type interval activated sludge reaction device and the method for utilizing its enrichment nitrifier and denitrifying bacterium Download PDFInfo
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Abstract
The present invention provides a kind of batch-type interval activated sludge reaction device, it includes reactor, the top of the reactor is sealed using plastic pad, which further includes magnetic stirring apparatus, pH value measurement indicator, dissolved oxygen sensing indicator, blast aeration machine, nitrogen supply (NS) pipeline, intake pump, water outlet pump and temperature survey indicator；The magnetic stirring apparatus is arranged at the bottom of the reactor, pH value measurement indicator, dissolved oxygen sensing indicator and temperature survey indicator are respectively used to measure the pH value of liquid, dissolved oxygen value and temperature value in the reactor, the intake pump and water outlet pump are respectively used to the reactor plus water draws water, and the blast aeration machine and nitrogen supply (NS) pipeline are respectively used to supply air and nitrogen to the reactor.The present invention also provides the method using reaction unit enrichment nitrifier and denitrifying bacterium.Present invention process flow and simple in structure, easy to operate, good to pernicious gas reduction, highly practical, prospect is extensive.
Description
Technical field
The invention belongs to sewage disposal control field, more particularly to a kind of batch-type interval activated sludge reaction device and profit
The method of nitrifier and denitrifying bacterium is enriched with it.
Background technology
N2O is a kind of important greenhouse gases in air, its greenhouse effects is about CO2310 times.In addition, N2O is mesh
Before the main reason for causing depletion of the ozone layer, its presence in an atmosphere can also form acid rain.Some researches show that sewage disposal
Biological denitrification process be N2One of main source of O.Therefore, N during research bio-denitrifying sewage2The mechanism of production of O, carries
Go out effective and feasible N2O minimizings control strategy has positive effect.
In bio-denitrifying sewage processing, the removal of nitrogen is mainly reacted by the nitrification and denitrification of microorganism.Research has shown that,
N can be produced in nitrifying process and denitrification process2O and NO and NO2.Give full play to the work of nitrifier and denitrifying bacterium
With so as to fulfill N2O and NO and NO2Minimizing, the matter of utmost importance to be solved is that nitrifier and denitrifying bacterium are enriched with
Culture, and service condition is adjusted to reach optimal operating condition.
SBR techniques have the advantages that technological process is simple, and treatment effect is stablized, and operation is easy, its basic principle is good
The operating condition of ammonia nitrogen process under the conditions of oxygen in sewage is converted into nitrate nitrogen and nitrite nitrogen by nitrification, is lacking
N is changed into by the approach of denitrification and short-cut denitrification under conditions of oxygen2。
Traditional N2O and NO and NO2Most of minimizing control strategy is so as to nitrification in the service condition for exploring technique
And denitrification process is improved, still, these control measure can be to system while pernicious gas minimizing is realized
Nitrogen removal performance causes certain adverse effect.And by adjusting microbiological paper method and on this basis Reasonable Regulation And Control is run
Operating mode on the basis of efficient denitrification is ensured so that realize N2O and NO and NO2The minimizing research of gas is very few.
The content of the invention
Realized in view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide one kind on the basis of efficient denitrification is ensured
N2O and NO and NO2The batch-type interval activated sludge reaction device of gas minimizing and its enrichment nitrifier of utilization and denitrification
The method of bacterium.
The present invention is that scheme is used by solving above-mentioned technical problem:
A kind of batch-type interval activated sludge reaction device, it includes reactor, it is characterised in that the top of the reactor
Portion is sealed using plastic pad, and the batch-type interval activated sludge reaction device further includes magnetic stirring apparatus, pH value measurement instruction
Device, dissolved oxygen sensing indicator, blast aeration machine, nitrogen supply (NS) pipeline, intake pump, water outlet pump and temperature survey indicator；Institute
State the bottom that magnetic stirring apparatus is arranged at the reactor, pH value measurement indicator, dissolved oxygen sensing indicator and the temperature
Measurement indicator is respectively used to measure the pH value of liquid, dissolved oxygen value and temperature value in the reactor, the intake pump and goes out
Water pump is respectively used to the reactor plus water draws water, and the blast aeration machine and nitrogen supply (NS) pipeline are respectively used to described
Reactor supplies air and nitrogen.
In such scheme, the batch-type interval activated sludge reaction device further includes inlet bucket and out of the bucket, it is described into
Water pump is positioned in the inlet bucket, and the water outlet pump is positioned in the out of the bucket.
In such scheme, the batch-type interval activated sludge reaction device is further included for controlling the reactor liquid level
Liquid level relay.
A kind of method that nitrifier and denitrifying bacterium are enriched with using batch-type interval activated sludge reaction device, it is included such as
Lower step：
1) seed sludge after domestication is transferred to anti-equipped with waste water in batch-type interval activated sludge reaction device
Answer in device；
2) the aerobic and pattern of anoxic alternate run enrichment nitrifier and denitrifying bacterium are taken；
3) operating condition is further optimized under conditions of nitrifier and denitrifying bacterium have high activity；
4) loading factor C/N ratios and DO values are changed on the basis of stable operation after reactor optimization, to NO, NO2、N2O
Escaped quantity and total nitrogen decrement measure.
In such scheme, the aerobic section dissolved oxygen in the step 2) is controlled in 2.0~2.6mg/L, anoxic section dissolved oxygen
Control is in 0.1~0.7mg/L.
In such scheme, the aerobic section time is 6h in the step 2), and the anoxic section time is 5h, sedimentation time 0.5h, into,
Water discharging time is 0.25h.
In such scheme, the pH value of waste water is 7~8, and water temperature is 25~35 DEG C.
In such scheme, using the carbon source and nitrogen of sodium acetate and ammonium chloride as the sludge after domestication in the step 1)
Source, C/N ratios are 3-8.
In such scheme, the aerobic section dissolved oxygen in the step 2) is controlled in 2.0~2.6mg/L, anoxic section dissolved oxygen
It is 8 to control the C/N ratios in 0.3mg/L, the step 1).
To ensure the good running environment of reactor, with thermostatical circulating water to maintain the temperature of reaction solution around reactor
Degree.For waste water used for the strong chemical production wastewater (water quality table 1) of Practical Project Yichang China, the sludge being inoculated with is dirty for the enterprise
Anoxic zone sludge (sludge parameter list 2) during engineering of water treatment operational excellence.
The strong waste water quality parameter list of the China of table 1
| Parameter | CODcr | TN | C | NO3 --N | NO2 --N | pH |
| Raw water quality | 500~800 | 300~600 | 250~500 | 50~100 | <10 | 7.20~8.62 |
2 anoxic zone sludge parameter list of table
| Color | Form | MLSS(mg/L) | MLVSS(mg/L) | SVI(ml/g) | SV30(%) |
| It is deep yellow | Cotton-shaped, nothing crushes | 4251 | 4067 | 63.5 | 27 |
Beneficial effects of the present invention are：Technological process is simple, simple in structure, easy to operate, and pernicious gas minimizing is imitated
Fruit is good, highly practical, and prospect is extensive.
Brief description of the drawings
Fig. 1 is the structure diagram of the batch-type interval activated sludge reaction device described in the embodiment of the present invention.
Fig. 2 is nitrifier electromicroscopic photograph after stablizing.
Fig. 3 is denitrifying bacterium electromicroscopic photograph after stablizing.
In figure：1- organic glass cylindrical reactors；2- magnetic stirring apparatus；3-pH values measure indicator；4-DO is measured
Indicator；5- plastic pads；6- temperature survey indicators；7- collection gas ports；8- high pure nitrogen supply lines；9- spinner flowmeters；10-
Blast aeration machine；11- intake pumps；12- water outlet pumps；13- liquid level relays；14- inlet buckets；15- out of the bucket.
Embodiment
Below in conjunction with drawings and examples, further the present invention will be described, but present disclosure is not limited solely to
The following examples.
Embodiment 1
The present invention is that aerobic/anoxic pattern is run in homemade batch-type interval activated sludge reaction device, is established high
The nitrifier of selectivity and the culture environment of denitrifying bacterium are spent, so as to fulfill the nitrifier in sbr reactor device and denitrifying bacterium
Enrichment.And carry out searching system optimal operating condition by adjusting loading factor C/N and DO value so as to fulfill in denitrification process
Pernicious gas minimizing.
As shown in Figure 1, pernicious gas subtracts during it is used for realization sewage water denitrification for one kind provided in an embodiment of the present invention
The batch-type interval activated sludge reaction device (abbreviation SBR) of quantization, it includes organic glass system cylindrical reactor 1, described
The top of reactor is sealed using plastic pad 5, the batch-type interval activated sludge reaction device further include magnetic stirring apparatus 2,
PH value measurement indicator 3, dissolved oxygen sensing indicator 4, blast aeration machine 10, nitrogen supply (NS) pipeline 8, intake pump 11, water outlet pump
12 and temperature survey indicator 6.Magnetic stirring apparatus 2 is arranged at the bottom of the reactor 1, the pH value measurement indicator 3, molten
Solution oxygenation measurement indicator 4 and temperature survey indicator 6 are respectively used to measure the pH value of liquid, dissolved oxygen value in the reactor 1
And temperature value.Intake pump 11 and water outlet pump 12 are respectively used to the reactor 1 plus water draws water, 10 He of blast aeration machine
Nitrogen supply (NS) pipeline 8 is respectively used to supply air and nitrogen to the reactor 1.Blast aeration machine 10 passes through spinner flowmeter 9
Adjust aeration quantity.The dischargeable capacity of reactor 1 in the present embodiment is 2L.
The present embodiment also provides the side that nitrifier and denitrifying bacterium are enriched with using batch-type interval activated sludge reaction device
Method, using the carbon source and nitrogen source of sodium acetate and ammonium chloride as the sludge after domestication, C/N ratios are 3.Water inlet NH4 +- N is 50mg/L；
COD is 150mg/L；Aerobic section DO is 2.0~2.6mg/L；PH is 7.0~7.5.The sbr reactor device aerobic section time is 6h, anoxic
The section time is 5h, and sedimentation time 0.5h, the water-in and water-out time is 0.25h, and water temperature is 28~30 DEG C.NO、NO2、N2The maximum ease of O
Output is respectively 0.827mg, 1.209mg, 4.207mg, and three kinds of total escaped quantities of gas are 6.243mg, N2The escaped quantity of O is accounted for into water
TN ratios are 4.207%；The decrement of total nitrogen is 53.79mg, and anoxic zone denitrification percent is 43.55%, and total denitrification percent is
53.79%.
Embodiment 2
The batch-type interval activated sludge reaction device of the present embodiment is same as Example 1, and difference is to utilize sequence
Batch interval activated sludge reaction device is enriched with nitrifier and the method for denitrifying bacterium is：Using sodium acetate and ammonium chloride as warp
The carbon source and nitrogen source of sludge after domestication, C/N ratios are 4.Water inlet NH4 +- N is 50mg/L；COD is 200mg/L；Aerobic section DO is 2.0
~2.6mg/L；PH is 7.0~7.5.The sbr reactor device aerobic section time is 6h, and the anoxic section time is 5h, sedimentation time 0.5h,
The water-in and water-out time is 0.25h, and water temperature is 28~30 DEG C.NO、NO2And N2The maximum escaped quantity of O be respectively 0.542mg,
0.885mg and 2.808mg, three kinds of total escaped quantities of gas are 4.235mg, N2It is 2.808% that the escaped quantity of O, which is accounted for into water TN ratios,；
The decrement of total nitrogen is 70.01mg, and anoxic zone denitrification percent is 58.24%, and total denitrification percent is 70.01%.
Embodiment 3
The batch-type interval activated sludge reaction device of the present embodiment is same as Example 1, and difference is to utilize sequence
Batch interval activated sludge reaction device is enriched with nitrifier and the method for denitrifying bacterium is：Using sodium acetate and ammonium chloride as warp
The carbon source and nitrogen source of sludge after domestication, C/N ratios are 6.Water inlet NH4 +- N is 50mg/L；COD is 300mg/L；Aerobic section DO is 2.0
~2.6mg/L；PH is 7.0~7.5.The sbr reactor device aerobic section time is 6h, and the anoxic section time is 5h, sedimentation time 0.5h,
The water-in and water-out time is 0.25h, and water temperature is 28~30 DEG C.NO、NO2And N2The maximum escaped quantity of O be respectively 0.550mg,
0.895mg and 2.565mg, three kinds of total escaped quantities of gas are 4.010mg, N2It is 2.565% that the escaped quantity of O, which is accounted for into water TN ratios,；
The decrement of total nitrogen is 73.38mg, and anoxic zone denitrification percent is 61.07%, and total denitrification percent is 73.38%.
Embodiment 4
The batch-type interval activated sludge reaction device of the present embodiment is same as Example 1, and difference is to utilize sequence
Batch interval activated sludge reaction device is enriched with nitrifier and the method for denitrifying bacterium is：Using sodium acetate and ammonium chloride as warp
The carbon source and nitrogen source of sludge after domestication, C/N ratios are 8.Water inlet NH4 +- N is 50mg/L；COD is 400mg/L；Aerobic section DO is 2.0
~2.6mg/L；PH is 7.0~7.5.The sbr reactor device aerobic section time is 6h, and the anoxic section time is 5h, sedimentation time 0.5h,
The water-in and water-out time is 0.25h, and water temperature is 28~30 DEG C.NO、NO2And N2The maximum escaped quantity of O be respectively 0.547mg,
0.887mg and 2.457mg, three kinds of total escaped quantities of gas are 3.891mg, N2It is 2.457% that the escaped quantity of O, which is accounted for into water TN ratios,；
The decrement of total nitrogen is 74.28mg, and anoxic zone denitrification percent is 62.74%, and total denitrification percent is 74.28%.
Embodiment 5
The batch-type interval activated sludge reaction device of the present embodiment is same as Example 1, and difference is to utilize sequence
Batch interval activated sludge reaction device is enriched with nitrifier and the method for denitrifying bacterium is：Using sodium acetate and ammonium chloride as warp
The carbon source and nitrogen source of sludge after domestication, C/N ratios are 8, and it is 2.0~2.6mg/L to make aerobic section DO；Anoxic section DO values are 0.7mg/L.
Water inlet NH4 +- N is 50mg/L；COD is 200mg/L；PH is 7.0~7.5.The sbr reactor device aerobic section time is 6h, during anoxic section
Between be 5h, sedimentation time 0.5h, the water-in and water-out time is 0.25h, and water temperature is 28~30 DEG C.NO、NO2And N2Total escaped quantity of O
Respectively 0.776mg, 1.214mg and 3.294mg, three kinds of total escaped quantities of gas are 5.284mg, N2The escaped quantity of O is accounted for into water TN
Ratio is 3.294%；The decrement of total nitrogen is 47.12mg, and anoxic zone denitrification percent is 38.98%, and total denitrification percent is 47.12%.
Embodiment 6
The batch-type interval activated sludge reaction device of the present embodiment is same as Example 1, and difference is to utilize sequence
Batch interval activated sludge reaction device is enriched with nitrifier and the method for denitrifying bacterium is：Using sodium acetate and ammonium chloride as warp
The carbon source and nitrogen source of sludge after domestication, C/N ratios are 8, and it is 2.0~2.6mg/L to make aerobic section DO；Anoxic section DO values are 0.5mg/L.
Water inlet NH4 +- N is 50mg/L；COD is 200mg/L；PH is 7.0~7.5.The sbr reactor device aerobic section time is 6h, during anoxic section
Between be 5h, sedimentation time 0.5h, the water-in and water-out time is 0.25h, and water temperature is 28~30 DEG C.NO、NO2And N2Total escaped quantity of O
Respectively 0.578mg, 0.849mg and 2.347mg, three kinds of total escaped quantities of gas are 3.774mg, N2The escaped quantity of O is accounted for into water TN
Ratio is 2.347%；The decrement of total nitrogen is 67.30mg, and anoxic zone denitrification percent is 60.80%, and total denitrification percent is 67.30%.
Embodiment 7
The batch-type interval activated sludge reaction device of the present embodiment is same as Example 1, and difference is to utilize sequence
Batch interval activated sludge reaction device is enriched with nitrifier and the method for denitrifying bacterium is：Using sodium acetate and ammonium chloride as warp
The carbon source and nitrogen source of sludge after domestication, C/N ratios are 8, and it is 2.0~2.6mg/L to make aerobic section DO；Anoxic section DO values are 0.3mg/L.
Water inlet NH4 +- N is 50mg/L；COD is 200mg/L；PH is 7.0~7.5.The sbr reactor device aerobic section time is 6h, during anoxic section
Between be 5h, sedimentation time 0.5h, the water-in and water-out time is 0.25h, and water temperature is 28~30 DEG C.NO、NO2And N2Total escaped quantity of O
Respectively 0.417mg, 0.619mg and 1.240mg, three kinds of total escaped quantities of gas are 2.276mg/L, N2The escaped quantity of O is accounted for into water
TN ratios are 1.240%；The decrement of total nitrogen is 72.70mg, and anoxic zone denitrification percent is 70.49%, and total denitrification percent is
72.70%.
Embodiment 8
The batch-type interval activated sludge reaction device of the present embodiment is same as Example 1, and difference is to utilize sequence
Batch interval activated sludge reaction device is enriched with nitrifier and the method for denitrifying bacterium is：Using sodium acetate and ammonium chloride as warp
The carbon source and nitrogen source of sludge after domestication, C/N ratios are 8, and it is 2.0~2.6mg/L to make aerobic section DO；Anoxic section DO values are 0.1mg/L.
Water inlet NH4 +- N is 50mg/L；COD is 200mg/L；PH is 7.0~7.5.The sbr reactor device aerobic section time is 6h, during anoxic section
Between be 5h, sedimentation time 0.5h, the water-in and water-out time is 0.25h, and water temperature is 28~30 DEG C.NO、NO2And N2Total escaped quantity of O
Respectively 0.540mg, 0.801mg and 1.185mg, three kinds of total escaped quantities of gas are 2.526mg/L, N2The escaped quantity of O is accounted for into water
TN ratios are 1.185%；The decrement of total nitrogen is 36.90mg, and anoxic zone denitrification percent is 33.03%, and total denitrification percent is
36.90%.
Claims (6)
1. a kind of carry out N using batch-type interval activated sludge reaction device2O and NO and NO2The side of pernicious gas minimizing
Method, it is characterised in that it includes the following steps：
1）Seed sludge after domestication is transferred to the reactor that waste water is housed in batch-type interval activated sludge reaction device
It is interior, wherein, using the carbon source and nitrogen source of sodium acetate and ammonium chloride as the sludge after domestication, C/N ratios are 3-8；
2）The aerobic and pattern of anoxic alternate run enrichment nitrifier and denitrifying bacterium are taken, wherein, the aerobic section time is 6h, is lacked
The oxygen section time is 5h, and sedimentation time 0.5h, the water-in and water-out time is 0.25h；Aerobic section dissolved oxygen is controlled in 2.0~2.6mg/
L, anoxic section dissolved oxygen are controlled in 0.1~0.7mg/L；
3) operating condition is further optimized under conditions of nitrifier and denitrifying bacterium have high activity；
4) loading factor C/N ratios and DO values are changed on the basis of stable operation after reactor optimization, to NO, NO2、N2O's
Escaped quantity and total nitrogen decrement measure.
2. the method as described in claim 1, it is characterised in that the pH value of waste water is 7~8, and water temperature is 25~35 DEG C.
3. the method as described in claim 1, it is characterised in that the step 2）In the control of aerobic section dissolved oxygen 2.0~
2.6mg/L, anoxic section dissolved oxygen are controlled in 0.3mg/L, the step 1）In C/N ratios be 8.
4. the method as described in claim 1, it is characterised in that the batch-type interval activated sludge reaction device includes reaction
Device, the top of the reactor are sealed using plastic pad, and the batch-type interval activated sludge reaction device further includes magnetic force and stirs
Mix device, pH value measurement indicator, dissolved oxygen sensing indicator, blast aeration machine, nitrogen supply (NS) pipeline, intake pump, water outlet pump and
Temperature survey indicator；The magnetic stirring apparatus is arranged at the bottom of the reactor, the pH value measurement indicator, dissolved oxygen
Measurement indicator and temperature survey indicator are respectively used to measure the pH value of liquid, dissolved oxygen value and temperature in the reactor
Value, the intake pump and water outlet pump are respectively used to the reactor plus water or draws water, the blast aeration machine and nitrogen supply (NS)
Pipeline is respectively used to supply air and nitrogen to the reactor, and the top of the reactor is taper.
5. method as claimed in claim 4, it is characterised in that the batch-type interval activated sludge reaction device further include into
Bucket and out of the bucket, the intake pump are positioned in the inlet bucket, and the water outlet pump is positioned in the out of the bucket.
6. method as claimed in claim 4, it is characterised in that the batch-type interval activated sludge reaction device further includes use
In the liquid level relay for controlling the reactor liquid level.
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