CN104604757B - A kind of Portunus trituberculatus Miers fry quality detection technique - Google Patents
A kind of Portunus trituberculatus Miers fry quality detection technique Download PDFInfo
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- CN104604757B CN104604757B CN201410245722.3A CN201410245722A CN104604757B CN 104604757 B CN104604757 B CN 104604757B CN 201410245722 A CN201410245722 A CN 201410245722A CN 104604757 B CN104604757 B CN 104604757B
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- quality
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- fry quality
- portunus trituberculatus
- trituberculatus miers
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- 241001533364 Portunus trituberculatus Species 0.000 title claims abstract description 18
- 238000001514 detection method Methods 0.000 title claims abstract description 11
- 230000007613 environmental effect Effects 0.000 claims abstract description 5
- 230000000366 juvenile effect Effects 0.000 abstract description 21
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 13
- 230000001665 lethal effect Effects 0.000 abstract description 11
- 231100000518 lethal Toxicity 0.000 abstract description 10
- 150000002500 ions Chemical class 0.000 abstract description 9
- 238000013461 design Methods 0.000 abstract description 5
- 238000002474 experimental method Methods 0.000 abstract description 5
- 238000009360 aquaculture Methods 0.000 abstract description 3
- 244000144974 aquaculture Species 0.000 abstract description 3
- 238000011156 evaluation Methods 0.000 abstract description 2
- 230000004069 differentiation Effects 0.000 abstract 1
- 230000002262 irrigation Effects 0.000 abstract 1
- 238000003973 irrigation Methods 0.000 abstract 1
- 238000013441 quality evaluation Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 description 5
- 231100000419 toxicity Toxicity 0.000 description 5
- 230000001988 toxicity Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 4
- 241000371997 Eriocheir sinensis Species 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 241000238557 Decapoda Species 0.000 description 2
- 231100000215 acute (single dose) toxicity testing Toxicity 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 229940126678 chinese medicines Drugs 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 2
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- RZLVQBNCHSJZPX-UHFFFAOYSA-L zinc sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Zn+2].[O-]S([O-])(=O)=O RZLVQBNCHSJZPX-UHFFFAOYSA-L 0.000 description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 229910052927 chalcanthite Inorganic materials 0.000 description 1
- 239000003653 coastal water Substances 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- BXRRQHBNBXJZBQ-UHFFFAOYSA-L dichloromanganese;hydrate Chemical compound O.Cl[Mn]Cl BXRRQHBNBXJZBQ-UHFFFAOYSA-L 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid group Chemical class S(O)(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention is a kind of Portunus trituberculatus Miers fry quality detection technique, it is that the detection method of fry quality is detected with the inverse property environmental system of different heavy metal ion composition, belong to Aquaculture Science field, it is characterised in that obtain an inverse property environmental system for being suited for distinguishing between different fry qualities using Orthogonal Experiment and Design and can distinguish the detection time scope of different fry quality differences using this system.Its drip irrigation device is that the inverse property environmental system of a differentiation juvenile horse crab vigor is obtained first with Orthogonal Experiment and Design：Fe2+For 4.47mg/L, Mn2+For 56.23mg/L, Hg2+For 0.5mg/L, Cu2+For 5.01mg/L and Zn2+For 10mg/L；Secondly, the index of an evaluation fry quality is obtained using the system, i.e. the lethal time fry quality of 0 2.5 hours is poor；The lethal time fry quality of 2.6 3.5 hours is medium；Lethal time more than 3.5 hours for high-quality seed.The fry quality evaluation that this invention can put before seedling for Portunus trituberculatus Miers pond culture provides quantitative basis.
Description
Technical field：
It is that one kind utilizes Portunus trituberculatus Miers seed the present invention relates to a kind of detection method of Portunus trituberculatus Miers fry quality
The marine aquaculture fry quality detection technique that endurance for adverse circumstance environment is different and develops, belongs to Aquaculture Science
Field.
Background technology：
With the increasingly reduction of coastal water fishery resource, Portunus trituberculatus Miers (Portunus trituberculatus) seawater is supported
Grow and developed rapidly already in national coastal area, cultured area constantly expands, juvenile crab production turns into the main body of each nursery producer
One of industry, fry quality quality, be directly connected to the economic destiny of each nursery producer, be also turn into be related to raiser can
Cultivate one of successful key factor.But in culture fishery, so far also without effective more Accurate Assessment water
The method of production cultivation fry quality quality.
How to select, be currently concentrated mainly in the research of Eriocheir sinensis for crab class seed.The gentle Yang Li rosy clouds of He Wen
(2005) think, the discriminating of Eriocheir sinensis juvenile crab quality, can be carried out from the following aspects：1st, the mistake of juvenile crab production is understood
Journey：When buying juvenile crab, if the juvenile crab of artificial propagation is it is necessary to understanding age in days, the bait feeding situation of juvenile crab, desalt processing mistake
Situations such as journey.General feeding management is good, and juvenile crab age in days was up to more than 5 days, by multiple desalt processing, and seed density is larger in pond,
And it is relatively active, it is consistent with a collection of juvenile crab size specification, typically require that 80%-90% specifications are identical, illustrate juvenile crab quality compared with
It is good, it is on the contrary then poor；2nd, appearance is observed：High-quality juvenile crab body colour is yellowish, slightly band gloss, and crab pond is free of dead seedling debris, juvenile crab activity
Ability is strong, and reaction is flexible, after juvenile crab is drained the water, is held by hand one and gently one holds, and release is manually placed into juvenile crab case, if juvenile crab
It can immediately scatter, creep rapid, then quality is preferable；3rd, survey sample：Juvenile crab is picked up into a part, moisture is drained, claims 1-2 grams of crab
Seedling counts, and specification reaches/kilogram of 14-16 ten thousand, illustrates that juvenile crab quality preferably, can be bought；4th, simulated test：Pond will be gone out
Juvenile crab weigh 1-2 grams, wrapped with wet gauze, be placed on shady place, checked after the storage of 8-10 hour, if more than 80%
Juvenile crab is all living, illustrates that quality is preferable.
From the above, it can be seen that what the detection for fry quality relied primarily on is Conventional wisdom, these experiences can be fast
Preliminary estimation fastly is carried out to fry quality, but explication de texte can not be accomplished.In fact, the excellent seed master that we often say
It is embodied in two aspects, one is that the death rate is low in breeding process, and two be that the speed of growth is very fast.The quality of fry quality is except product
Outside species diversity, the influence of various environmental factors in cultivating process is depended primarily on.Different envirment factors is for Shrimp waste seed
The influence of cultivation is different.Xu Jianrong etc. (2006) research shows that salinity, bait, illumination etc. are big to Eriocheir sinensis
Eye young degenerate cllipticity, which has, to be significantly affected.Therefore, this gives the thinking for we providing detection fry quality, you can to pass through inspection
Different seeds are surveyed for the resistance degree of the adverse environment factor to determine the quality of seed, and the investigative technique of this respect exists at present
Both at home and abroad also in space state.
The content of the invention：
The present invention be description using Portunus trituberculatus Miers seed under inverse property environment the difference of time-to-live length and set up
A kind of fry quality Examined effect come, solves raiser and fry quality is estimated when seedling is put in Portunus trituberculatus Miers cultivation
This problem, main contents are：
First, the selection of heavy metal ion and concentration setting
Inverse property environment is provided using the toxicity of heavy metal ion, specifically chosen following chemical reagent is used as heavy metal ion
Source：Copper sulphate (CuSO4·5H2O) (Chemical Reagent Co., Ltd., Sinopharm Group), ferrous sulfate (FeSO4) (Chinese medicines group
Learn reagent Co., Ltd), four chloride hydrate manganese (Mncl2.4H2O) (Chemical Reagent Co., Ltd., Sinopharm Group), seven hydrated sulfuric acids
Zinc (ZnSO4.7H2O) (Chemical Reagent Co., Ltd., Sinopharm Group), mercury bichloride (Hgcl2) (Chinese medicines group chemical reagent is limited
Company), zinc sulfate (ZnSO4.7H2O) (Chemical Reagent Co., Ltd., Sinopharm Group).
Each ion sets 5 gradient concentrations respectively, i.e., 5 levels are specifically shown in Table 1.
Each concentration of heavy metal ion gradient of table 1
2nd, Orthogonal Experiment and Design scheme
Using list of the factor orthogonal trial the above heavy metal ion of 6 factor 5 for Portunus trituberculatus Miers second phase young crab
Only or joint toxicity, sets 3 repetitions and 3 controls altogether.
Orthogonal test table is set following (being shown in Table 2)：
The orthogonal test designs table of table 2
3 repetitions and 3 controls are set altogether.
3rd, acute toxicity testing of the heavy metal to Portunus trituberculatus Miers second phase young crab
The growth of Portunus trituberculatus Miers seed can just be sold to raiser's cultivation when reaching the second stage of young crab, therefore be selected in this experiment
The second stage of children crab is used as subject.25 beakers are taken, are placed in bubble chamber, and 25 DEG C of keeping temperature, successively by above-mentioned table 2
Various Heavy Metal Reagents are added per a line and corresponding metal ion is reached corresponding concentration, each beaker puts 5 young crabs, often
Every dead individuals situation of 2 hour record.
The foundation for weighing lethal effect, the calculation formula of average death time (Dt) are used as using average death time：
Wherein, Dt is average death time, niFor the i-th time dead number, t is the death time of correspondence individual, and N is total
Dead individuals number.
Carried out using orthogonal design assistant IIV3.1 professional versions software and SPSS14.0 (SPSS Inc., Chicago, IL)
Data analysis, concrete outcome is shown in Table the experimental result in 2.
Can intuitively it be learnt from result, the lethal time of 25 groups of experiment is most short, i.e. Cu2+For 5.01mg/L, Zn2+For 10mg/
L, Mn2+For 56.23mg/L, Hg2+For 0.16mg/L, Fe2+During for 2.11mg/L, the combinations of these ions to the toxicity of young crab most
By force.
4th, the optimal combination of heavy metals concentration that juvenile horse crab vigor is distinguished
According to the experimental result of table 2, each metal ion of analysis shows directly perceived is followed successively by Fe to the toxicity size of young crab2+＞ Mn2+
＞ Hg2+＞ Cu2+＞ Zn2+.By variance analysis, the toxicity size of variant concentration heavy metal is obtained, further obtaining can be with area
Divide the heavy metal ion combined system of juvenile horse crab vigor difference：Fe2+For 4.47mg/L, Mn2+For 56.23mg/L, Hg2 +For 0.5mg/L, Cu2+For 5.01mg/L and Zn2+For 10mg/L.
5th, the selection for the lethal time point that the different young crab quality of Portunus trituberculatus Miers family have differences
15 familys are chosen as tested family, combination of heavy metals concentration (Fe is utilized2+For 4.47mg/L, Mn2+For
56.23mg/L、Hg2+For 0.5mg/L, Cu2+For 5.01mg/L and Zn2+For 10mg/L) respectively to these family Portunus trituberculatus Miers children
Crab carries out acute toxicity testing, and the time point of 15 family fry qualities can be distinguished by obtaining：Lethal time is 0-2.5 hours
Fry quality it is poor；Lethal time is medium for the fry quality of 2.6-3.5 hours；Lethal time was more than 3.5 hours
High-quality seed.
The checking of the testing result of fry quality
15 familys carry out the comparing check of growth traits and survival rate under identical breeding environment to more than, as a result table
It is bright：The fry quality that the lethal time detected above is defined and these survivals of family seed in actual breeding process
Rate and growth traits characteristic are consistent, illustrate to utilize combination of heavy metals concentration (Fe2+For 4.47mg/L, Mn2+For 56.23mg/L,
Hg2+For 0.5mg/L, Cu2+For 5.01mg/L and Zn2+For 10mg/L) carry out fry quality detection and according to lethal time " 0-2.5
Hour ", " 2.6-3.5 hours " with " " evaluation criterion that three standards divide fry qualities was feasible, reliable in more than 3.5 hours
's.
Claims (1)
1. Portunus trituberculatus Miers fry quality detection method
Portunus trituberculatus Miers second phase young crab is chosen to be put into as in subinverse environmental system：Fe2+For 4.47mg/L, Mn2+For 56.23mg/
L、Hg2+For 0.5mg/L, Cu2+For 5.01mg/L and Zn2+For 10mg/L, the death time of young crab is then observed and records, according to
Following index judges the fry quality of Portunus trituberculatus Miers：Death time scope is poor for the fry quality of 0-2.5 hours；When dead
Between scope it is medium for the fry quality of 2.6-3.5 hours；Death time scope more than 3.5 hours for high-quality seed.
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|---|---|---|---|---|
| CN102217566A (en) * | 2011-04-29 | 2011-10-19 | 宁波大学 | Pond polyculture method for pseudosciaena crocea and portunus trituberculatus |
| CN102613126A (en) * | 2012-04-26 | 2012-08-01 | 中国水产科学研究院黄海水产研究所 | High-efficient ecological seedling-raising method of blue crabs |
| CN102823533A (en) * | 2012-09-20 | 2012-12-19 | 中国水产科学研究院黄海水产研究所 | Low-salt overwintering and breeding method of portunus trituberculatus |
| CN103387978A (en) * | 2013-07-24 | 2013-11-13 | 浙江省淡水水产研究所 | Gene polymorphic marker of C-type lectin of portunus trituberculatus and genetic typing method of SNP (Single Nucleotide Polymorphism) molecular markers |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3669968B2 (en) * | 2002-03-25 | 2005-07-13 | 山形県 | Aquaculture equipment and method of use |
| JP2006288323A (en) * | 2005-04-13 | 2006-10-26 | Jfe Steel Kk | Artificial water bottom base |
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2014
- 2014-06-06 CN CN201410245722.3A patent/CN104604757B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102217566A (en) * | 2011-04-29 | 2011-10-19 | 宁波大学 | Pond polyculture method for pseudosciaena crocea and portunus trituberculatus |
| CN102613126A (en) * | 2012-04-26 | 2012-08-01 | 中国水产科学研究院黄海水产研究所 | High-efficient ecological seedling-raising method of blue crabs |
| CN102823533A (en) * | 2012-09-20 | 2012-12-19 | 中国水产科学研究院黄海水产研究所 | Low-salt overwintering and breeding method of portunus trituberculatus |
| CN103387978A (en) * | 2013-07-24 | 2013-11-13 | 浙江省淡水水产研究所 | Gene polymorphic marker of C-type lectin of portunus trituberculatus and genetic typing method of SNP (Single Nucleotide Polymorphism) molecular markers |
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| Cu、Pb、Cd、Hg亚致死浓度对三疣梭子蟹幼体的影响;廖永岩;《环境科学学报》;20070815;第27卷(第8期);全文 * |
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Effective date of registration: 20180112 Address after: 222000 Cangwu Road, Haizhou District, Jiangsu, No. 59, No. Co-patentee after: Ningbo University Patentee after: Huaihai Institute of Technology Address before: 222005 Cangwu Road, Sinpo District, Jiangsu, No. 59, No. Patentee before: Huaihai Institute of Technology |
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