CN104604757A - Quality detection technology for blue crab fries - Google Patents
Quality detection technology for blue crab fries Download PDFInfo
- Publication number
- CN104604757A CN104604757A CN201410245722.3A CN201410245722A CN104604757A CN 104604757 A CN104604757 A CN 104604757A CN 201410245722 A CN201410245722 A CN 201410245722A CN 104604757 A CN104604757 A CN 104604757A
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- CN
- China
- Prior art keywords
- quality
- fries
- detection technology
- crab
- adverse environmental
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 238000001514 detection method Methods 0.000 title claims abstract description 11
- 238000005516 engineering process Methods 0.000 title abstract description 6
- 241000238097 Callinectes sapidus Species 0.000 title abstract 4
- 230000007613 environmental effect Effects 0.000 claims abstract description 6
- 241001533364 Portunus trituberculatus Species 0.000 claims description 14
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 13
- 230000001665 lethal effect Effects 0.000 abstract description 11
- 231100000518 lethal Toxicity 0.000 abstract description 10
- 150000002500 ions Chemical class 0.000 abstract description 9
- 238000012360 testing method Methods 0.000 abstract description 7
- 230000002411 adverse Effects 0.000 abstract description 6
- 238000009360 aquaculture Methods 0.000 abstract description 4
- 244000144974 aquaculture Species 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 2
- 238000013441 quality evaluation Methods 0.000 abstract 1
- 230000000366 juvenile effect Effects 0.000 description 22
- 238000000034 method Methods 0.000 description 8
- 239000011701 zinc Substances 0.000 description 6
- 239000010949 copper Substances 0.000 description 5
- 239000011572 manganese Substances 0.000 description 5
- 231100000419 toxicity Toxicity 0.000 description 5
- 230000001988 toxicity Effects 0.000 description 5
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 241000238557 Decapoda Species 0.000 description 2
- 241000371986 Eriocheir Species 0.000 description 2
- 231100000215 acute (single dose) toxicity testing Toxicity 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 241001121957 Atelecyclidae Species 0.000 description 1
- 241000371997 Eriocheir sinensis Species 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003653 coastal water Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000010612 desalination reaction Methods 0.000 description 1
- BXRRQHBNBXJZBQ-UHFFFAOYSA-L dichloromanganese;hydrate Chemical compound O.Cl[Mn]Cl BXRRQHBNBXJZBQ-UHFFFAOYSA-L 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 231100000225 lethality Toxicity 0.000 description 1
- 238000009364 mariculture Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 235000009529 zinc sulphate Nutrition 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention provides a quality detection technology for blue crab fries. The quality detection technology is a detection method which uses an adverse environmental system composed of different heavy metal ions to detect fry quality, belonging to the field of aquaculture science. The quality detection technology is characterized in that orthogonal test is employed for designing and acquirement of the adverse environmental system applicable to distinguish of the quality of different fries; and the adverse environmental system can be applied to distinguish of the range of testing time of quality difference among the different fries. The main points of a technical scheme of the invention are as follows: the adverse environmental system capable of distinguishing the activity of young blue crabs is designed and obtained through orthogonal test, wherein in the system, the concentrations of Fe2+, Mn2+, Hg2+, Cu2+ and Zn 2+ are 4.47 mg/L, 56.23 mg/L, 0.5 mg/L, 5.01 mg/L and 10 mg/L, respectively; and the system is used to obtain one index used for evaluating the quality of the fries, i.e., the quality of fries with lethal time of 0 to 2.5 h is low, the quality of fries with lethal time of 2.6 to 3.5 h is medium, and the quality of fries with lethal time of more than 3.5 h is high. The technology can provide quantitative basis for quality evaluation of fries before releasing of blue crab fries for pond culture.
Description
Technical field:
The present invention relates to a kind of detection method of Portunus trituberculatus Miers fry quality, be a kind of marine aquaculture fry quality detection technique utilizing Portunus trituberculatus Miers seed to develop for the endurance difference of adverse circumstance environment, belong to Aquaculture Science field.
Background technology:
Along with the minimizing day by day of coastal water fishery resource, Portunus trituberculatus Miers (Portunus trituberculatus) mariculture developed rapidly in national coastal area already, cultured area constantly expands, juvenile crab produces has become one of autonomous industry of each nursery producer, fry quality quality, can be directly connected to the economic destiny of each nursery producer, be also become to be related to raiser and to cultivate one of successful key factor.But in culture fishery, also there is no the method for effective comparatively Accurate Assessment aquaculture fry quality quality so far.
How crab class seed is selected, currently mainly to concentrate in the research of Eriocheir sinensia.He Wen is gentle, and Yang Li rosy clouds (2005) are thought, the discriminating of Eriocheir sinensia juvenile crab quality, can carry out from the following aspects: 1, understand the process that juvenile crab produces: when buying juvenile crab, if the juvenile crab of artificial propagation, the age in days of juvenile crab, bait feeding situation will be understood, the situations such as desalination processing procedure.General feeding and management is good, and juvenile crab age in days reaches more than 5 days, and through repeatedly desalinating process, in pond, seed density is comparatively large, and more active, and consistent with a collection of juvenile crab size specification, General Requirements 80%-90% specification is identical, illustrates that juvenile crab quality is better, otherwise then poor; 2, observe appearance: high-quality juvenile crab body colour is yellowish, is slightly with gloss, crab pond is not containing dead seedling foreign material, and juvenile crab mobility is strong, reaction flexibly, after being drained the water by juvenile crab, is grabbed one with hand and one is held gently, unclamp hand and be put in juvenile crab case, if juvenile crab can scatter immediately, creep rapidly, then quality is better; 3, survey sample: juvenile crab is picked up a part, drains moisture, claims the numeration of 1-2 gram of juvenile crab, specification reaches 14-16 ten thousand/kilogram, illustrates that juvenile crab quality better, can buy; 4, constructed experiment: the juvenile crab that will go out pond takes 1-2 gram, wraps with wet gauze, puts in the cool, checks, if the juvenile crab of more than 80% is all alive, illustrate that quality is better through 8-10 hour after depositing.
As can be seen from the above, what the detection for fry quality mainly relied on is Conventional wisdom, and these experiences can carry out preliminary estimation to fry quality rapidly, but can not accomplish explication de texte.In fact, the excellent seed that we often say is mainly reflected in two aspects, and one is that in breeding process, lethality is low, and two is that growth rate is very fast.The quality of fry quality, except breed difference, depends primarily on the impact of various environmental factor in cultivating process.Different envirment factors is different for the impact of Shrimp waste seed rearing.The research of Xu Jianrong etc. (2006) shows, salinity, bait, illumination etc. have remarkable impact to Eriocheir sinensis megalopa degenerate cllipticity.Therefore, this detects the thinking of fry quality to we providing, and namely can to determine the quality of seed for the opposing degree of the adverse environment factor by detecting different seed, and the investigative technique of this respect is also in space state at home and abroad at present.
Summary of the invention:
The present invention describes a kind of fry quality Examined effect utilizing Portunus trituberculatus Miers seed to set up in the difference against time-to-live length under property environment, solve this problem that raiser assesses fry quality when seedling is put in Portunus trituberculatus Miers cultivation, main contents are:
One, the selection of heavy metal ion and concentration setting
Utilize the toxicity of heavy metal ion to provide inverse property environment, the following chemical reagent of concrete selection is as the source of heavy metal ion: copper sulphate (CuSO
45H
2o) (Chemical Reagent Co., Ltd., Sinopharm Group), ferrous sulfate (FeSO
4) (Chemical Reagent Co., Ltd., Sinopharm Group), four chloride hydrate manganese (Mncl
2.4H
2o) (Chemical Reagent Co., Ltd., Sinopharm Group), Zinc vitriol (ZnSO
4.7H
2o) (Chemical Reagent Co., Ltd., Sinopharm Group), mercury bichloride (Hgcl
2) (Chemical Reagent Co., Ltd., Sinopharm Group), zinc sulphate (ZnSO
4.7H
2o) (Chemical Reagent Co., Ltd., Sinopharm Group).
Each ion arranges 5 gradient concentrations respectively, i.e. 5 levels, specifically in table 1.
The each concentration of heavy metal ion gradient of table 1
Two, Orthogonal Experiment and Design scheme
Adopt these heavy metal ion of more than 6 factor 5 factor orthogonal trials for the toxicity alone or in combination of Portunus trituberculatus Miers second phase young crab, arrange 3 altogether and repeat and 3 contrasts.
Orthogonal test table is arranged (see table 2) as follows:
Table 2 orthogonal test designs table
Arrange 3 altogether to repeat and 3 contrasts.
Three, heavy metal is to the acute toxicity testing of Portunus trituberculatus Miers second phase young crab
The growth of Portunus trituberculatus Miers seed just can be sold to raiser's cultivation when reaching the second stage of young crab, therefore selects the second stage of young crab as tested object in this experiment.Get 25 beakers, be placed in bubble chamber, and keep temperature 25 DEG C, add various Heavy Metal Reagent by the every a line in above-mentioned table 2 successively and make corresponding metal ion reach corresponding concentration, each beaker puts 5 young crabs, every 2 hour record dead individuals situation.
Using mean time to death as the foundation weighing lethal effect, the computing formula of mean time to death (Dt):
Wherein, Dt is mean time to death, n
ibe the i-th time dead number, t is the corresponding individual death time, and N is total dead individuals number.
Utilize orthogonal design assistant IIV3.1 professional version software and SPSS14.0 (SPSS Inc., Chicago, IL) to carry out data analysis, concrete outcome is in the experimental result in table 2.
Can intuitively learn from result, the lethal time of testing 25 groups is the shortest, i.e. Cu
2+for 5.01mg/L, Zn
2+for 10mg/L, Mn
2+for 56.23mg/L, Hg
2+for 0.16mg/L, Fe
2+during for 2.11mg/L, the toxicity of combination to young crab of these ions is the strongest.
Four, the best combination of heavy metals concentration of juvenile horse crab vigor differentiation
According to table 2 experimental result, intuitive analysis shows that the toxicity size of each metal ion to young crab is followed successively by Fe
2+> Mn
2+> Hg
2+> Cu
2+> Zn
2+.Through variance analysis, obtain the toxicity size of variant concentration heavy metal, obtain the heavy metal ion combined system can distinguishing juvenile horse crab vigor difference further: Fe
2+for 4.47mg/L, Mn
2+for 56.23mg/L, Hg
2+for 0.5mg/L, Cu
2+for 5.01mg/L and Zn
2+for 10mg/L.
Five, the selection of lethal time point that there are differences of different Portunus trituberculatus Miers family children crab quality
Choose 15 familys as tested family, utilize combination of heavy metals concentration (Fe
2+for 4.47mg/L, Mn
2+for 56.23mg/L, Hg
2+for 0.5mg/L, Cu
2+for 5.01mg/L and Zn
2+for 10mg/L) respectively acute toxicity testing is carried out to these family juvenile horse crabs, obtain the time point can distinguishing 15 family fry quality: lethal time is that the fry quality of 0-2.5 hour is poor; Lethal time is that the fry quality of 2.6-3.5 hour is medium; Lethal time was high-quality seed more than 3.5 hours.
Six, the checking of the testing result of fry quality
Above 15 familys are carried out under identical breeding environment to the comparing check of growth traits and survival rate, result shows: survival rate in actual breeding process of the fry quality that the lethal time detected above defines and these family seeds and growth traits characteristic consistent, illustrate and utilize combination of heavy metals concentration (Fe
2+for 4.47mg/L, Mn
2+for 56.23mg/L, Hg
2+for 0.5mg/L, Cu
2+for 5.01mg/L and Zn
2+for 10mg/L) carry out fry quality detection and be feasible, reliable according to the evaluation criterion of lethal time " 0-2.5 hour ", " 2.6-3.5 hour " and " more than 3.5 hours " three standards division fry quality.
Claims (1)
1. Portunus trituberculatus Miers fry quality detection method
Choose the second stage of young crab of Portunus trituberculatus Miers to put into as subinverse environmental system: Fe
2+for 4.47mg/L, Mn
2+for 56.23mg/L, Hg
2+for 0.5mg/L, Cu
2+for 5.01mg/L and Zn
2+for 10mg/L, observe subsequently and record death time of young crab, judging the fry quality of Portunus trituberculatus Miers according to following index: death time scope is that the fry quality of 0-2.5 hour is poor; Death time scope is that the fry quality of 2.6-3.5 hour is medium; Death time scope was high-quality seed more than 3.5 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410245722.3A CN104604757B (en) | 2014-06-06 | 2014-06-06 | A kind of Portunus trituberculatus Miers fry quality detection technique |
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|---|---|---|---|
| CN201410245722.3A CN104604757B (en) | 2014-06-06 | 2014-06-06 | A kind of Portunus trituberculatus Miers fry quality detection technique |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104604757A true CN104604757A (en) | 2015-05-13 |
| CN104604757B CN104604757B (en) | 2017-10-10 |
Family
ID=53139633
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410245722.3A Expired - Fee Related CN104604757B (en) | 2014-06-06 | 2014-06-06 | A kind of Portunus trituberculatus Miers fry quality detection technique |
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| Country | Link |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003274793A (en) * | 2002-03-25 | 2003-09-30 | Yamagata Prefecture | Crab culturing apparatus and use thereof |
| JP2006288323A (en) * | 2005-04-13 | 2006-10-26 | Jfe Steel Kk | Artificial water bottom base |
| CN102217566A (en) * | 2011-04-29 | 2011-10-19 | 宁波大学 | Pond polyculture method for pseudosciaena crocea and portunus trituberculatus |
| CN102613126A (en) * | 2012-04-26 | 2012-08-01 | 中国水产科学研究院黄海水产研究所 | High-efficient ecological seedling-raising method of blue crabs |
| CN102823533A (en) * | 2012-09-20 | 2012-12-19 | 中国水产科学研究院黄海水产研究所 | Low-salt overwintering and breeding method of portunus trituberculatus |
| CN103387978A (en) * | 2013-07-24 | 2013-11-13 | 浙江省淡水水产研究所 | Gene polymorphic marker of C-type lectin of portunus trituberculatus and genetic typing method of SNP (Single Nucleotide Polymorphism) molecular markers |
-
2014
- 2014-06-06 CN CN201410245722.3A patent/CN104604757B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003274793A (en) * | 2002-03-25 | 2003-09-30 | Yamagata Prefecture | Crab culturing apparatus and use thereof |
| JP2006288323A (en) * | 2005-04-13 | 2006-10-26 | Jfe Steel Kk | Artificial water bottom base |
| CN102217566A (en) * | 2011-04-29 | 2011-10-19 | 宁波大学 | Pond polyculture method for pseudosciaena crocea and portunus trituberculatus |
| CN102613126A (en) * | 2012-04-26 | 2012-08-01 | 中国水产科学研究院黄海水产研究所 | High-efficient ecological seedling-raising method of blue crabs |
| CN102823533A (en) * | 2012-09-20 | 2012-12-19 | 中国水产科学研究院黄海水产研究所 | Low-salt overwintering and breeding method of portunus trituberculatus |
| CN103387978A (en) * | 2013-07-24 | 2013-11-13 | 浙江省淡水水产研究所 | Gene polymorphic marker of C-type lectin of portunus trituberculatus and genetic typing method of SNP (Single Nucleotide Polymorphism) molecular markers |
Non-Patent Citations (2)
| Title |
|---|
| 姚海富等: "四种重金属对三疣梭子蟹溞状幼体的急性毒性", 《集美大学学报》 * |
| 廖永岩: "Cu、Pb、Cd、Hg亚致死浓度对三疣梭子蟹幼体的影响", 《环境科学学报》 * |
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| Publication number | Publication date |
|---|---|
| CN104604757B (en) | 2017-10-10 |
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Inventor after: Gao Huan Inventor after: Yan Binlun Inventor after: Yu Fei Inventor after: Xu Jing Inventor after: Chen Jianhua Inventor after: Lai Xiaofang Inventor before: Gao Huan Inventor before: Yan Binlun Inventor before: Xu Jing Inventor before: Chen Jianhua Inventor before: Lai Xiaofang |
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Free format text: CORRECT: INVENTOR; FROM: GAO HUAN YAN BINLUN XU JING CHEN JIANHUA LAI XIAOFANG TO: GAO HUAN YAN BINLUN YU FEI XU JING CHEN JIANHUA LAI XIAOFANG |
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Effective date of registration: 20180112 Address after: 222000 Cangwu Road, Haizhou District, Jiangsu, No. 59, No. Co-patentee after: Ningbo University Patentee after: Huaihai Institute of Technology Address before: 222005 Cangwu Road, Sinpo District, Jiangsu, No. 59, No. Patentee before: Huaihai Institute of Technology |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
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Granted publication date: 20171010 Termination date: 20210606 |