CN104588405B - The microorganism of a kind of oil-polluted soils-electronic two-part restorative procedure - Google Patents
The microorganism of a kind of oil-polluted soils-electronic two-part restorative procedure Download PDFInfo
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Abstract
The invention belongs to technical field of environmental management, be specifically related to the microorganism electronic two-part restorative procedure of a kind of oil-polluted soils.With total petroleum hydrocarbons content in contaminated soil and the ratio of total content of organic carbon, carry out microorganism remediation and the conversion of electro reclamation two-part, and then reach the purpose of remedying oil-polluted soils.In the inventive method, microorganism is preferentially by the light component degradation of easily degraded in petroleum hydrocarbon, removes heavy constituent by the invigoration effect of electric field the most again, it is achieved the stage degraded of pollutant.This invention had both avoided the problem that pollutant can not thoroughly be degraded by independent microorganism, reduced again independent electro reclamation energy consumption.The inventive method remedying oil-polluted soils speed is fast, efficiency is high, low cost in employing, and more single microorganism remediation, electro reclamation improve efficiency 20 more than 40%.
Description
Technical field
The invention belongs to technical field of environmental management, be specifically related to the microorganism-electronic two-part restorative procedure of a kind of oil-polluted soils.
Background technology
Soil petroleum pollution has become the environmental problem that can not be ignored, and oil-polluted soils reparation at home and abroad receives the attention of height.Current existing recovery technique includes peripheral doses technology, chemical remediation technology and bioremediation technology.Bioremediation technology typically refers to microorganism remediation, is a kind of recovery technique grown up on the basis of microbial degradation, compared with physics, chemical remediation technology, has low cost, does not destroy soil environment, non-secondary pollution and the feature such as simple to operate.But microorganism remediation is affected bigger by soil pollutant characteristic, microorganism can alkane in preferential degradation petroleum hydrocarbon and aromatic hydrocarbons, and even can not degrade to the degradation efficiency of gum asphalt is extremely low, so cause alkane and aromatic hydrocarbons in soil drastically to decline, gum asphalt ratio increases.A large amount of accumulations of gum asphalt can suppress growth of microorganism, reduces the remediation efficiency of microorganism, and therefore, simple dependence biological method is difficult to thoroughly repair oil-polluted soils, needs to combine the removal of other technique heavy constituent.
Electric repairing technique is considered as the effective technology solving oil-polluted soils, and its ultimate principle is that the various electrokinesises utilizing electric field to produce strengthen the bioavailability of pollutant in underground environment, the activity of microorganism or direct mineralising Organic substance.Because this technology has, rehabilitation cost is low, removal efficiency high, receives the extensive concern of domestic and international soil remediation expert.Electric repairing technique has been applied to (such as PAHs, petroleum hydrocarbon etc.) during high-concentration hardly-degradable organic polluted soil is repaired in recent years, and achieves preferable repairing effect.But in order to reduce the simple electric repairing technique energy consumption that uses, therefore correlational study is more likely to combined remediation technology exploitation, other technologies the pollutant of combining to remove in soil will be carried out with electric repairing technique.For the most cost-effective, raising remediation efficiency, for the combination of bioremediation technology and electric repairing technique is compared to other method, more meet the needs of in-situ immobilization and Practical Project.
Soil organic matter is the general name of various carbon compounds in soil, is the basis of contaminated soil biological restoration, and its content and composition all play decisive role to feasibility and the remediation efficiency of biological restoration.Petroleum hydrocarbon is the organic compound of a kind of carbon containing, therefore after oil pollution, the organic carbon content in soil substantially increases, numerous studies show oil-polluted soils organic carbon content and oil content correlation, and organic carbon consumption decides biodegradation rate and palliating degradation degree.Therefore, ratio based on total petroleum hydrocarbons content in contaminated soil and total content of organic carbon determines microorganism remediation and the binding site of electro reclamation two-stage process, can be effectively connected by two-stage process, shortens repair time, the remediation efficiency of raising petroleum hydrocarbon.
Summary of the invention
It is an object of the invention to provide the microorganism-electronic two-part restorative procedure of a kind of oil-polluted soils.
For achieving the above object, the technical solution used in the present invention is:
The microorganism of a kind of oil-polluted soils-electronic two-part restorative procedure, with total petroleum hydrocarbons content in contaminated soil and the ratio of total content of organic carbon, carries out microorganism remediation and the conversion of electro reclamation two-part, and then reaches the purpose of remedying oil-polluted soils.
The transfer point of described microorganism remediation and electro reclamation two-part is that in contaminated soil, the ratio of total petroleum hydrocarbons content and total content of organic carbon is 0.6；
In soil, total petroleum hydrocarbons content/total content of organic carbon < when 0.6, uses the mode of microorganism remediation to process contaminated soil；
In soil during total petroleum hydrocarbons content/total content of organic carbon >=0.6, the mode of electro reclamation is used to process contaminated soil.
Two-part restorative procedure further,
(1) measuring total petroleum hydrocarbons content and total content of organic carbon in pending contaminated soil, < when 0.6, inoculation degradation bacteria strains carries out microorganism remediation in pending contaminated soil to total petroleum hydrocarbons content/total content of organic carbon；Total petroleum hydrocarbons content and the change of total content of organic carbon ratio in periodic monitoring soil during reparation；
In pending contaminated soil during total petroleum hydrocarbons content/total content of organic carbon>=0.6, in soil, add organic carbon make total petroleum hydrocarbons content/total content of organic carbon<0.6, and then employing microorganism remediation contaminated soil；Total petroleum hydrocarbons content and the change of total content of organic carbon ratio in periodic monitoring soil during reparation；
(2) during microorganism remediation when total petroleum hydrocarbons content/total content of organic carbon >=0.6, electro reclamation is started.
During described microorganism remediation, degradation bacteria is inoculated in contaminated soil, temperature is 15-30 DEG C, humidity be 15-35%, pH be 6.0-8.0, repair；Wherein, the inoculum concentration of degradation bacteria is 5-8 × 108Cfu/g dry ground；
Described degradation bacteria strains is Bacillus licheniformis PS5 (Bacillus licheniformis PS5), culture presevation number is CCTCC NO:M2011261, preservation date is on July 19th, 2011, and preservation mechanism is China typical culture collection center, and address is China. Wuhan. and Wuhan University.
Described organic carbon refer to humic acid, mud, fertilizer one or more.
During described electro reclamation, to soil apply unidirectional current, the voltage gradient of soil is 1-1.5V/cm, use polarity of electrode switching the method for operation, the polarity switching interval time is 5-10min, holding soil moisture be 15-35%, pH be 5.5-8.5.
Meanwhile, two-part restorative procedure is preferably applicable to soil petroleum hydrocarbon content less than 8% contaminated soil.
The invention have the advantage that ratio based on total petroleum hydrocarbons content in contaminated soil and total content of organic carbon determines microorganism remediation and the binding site of electro reclamation two-stage process, and biological restoration and the process conditions of electro reclamation before and after binding site, microorganism and electric repair method is made effectively to combine, first microorganism is preferentially by the light component degradation of easily degraded in petroleum hydrocarbon, heavy constituent is removed the most again, it is achieved the stage degraded of pollutant by the invigoration effect of electric field.The present invention had both avoided the problem that pollutant can not thoroughly be degraded by independent microorganism, reduced again independent electro reclamation energy consumption.The inventive method remedying oil-polluted soils speed is fast, efficiency is high, low cost in employing, can effectively shorten oil-polluted soils and process the time, and more single microorganism remediation, electro reclamation improve more than efficiency 20-40%.
Accompanying drawing explanation
Total petroleum hydrocarbons content/total content of organic carbon ratio change and removal rate of petroleum hydrocarbons variation diagram in the Liaohe Oil Field soil that Fig. 1 provides for the embodiment of the present invention.
Total petroleum hydrocarbons content/total content of organic carbon ratio change and removal rate of petroleum hydrocarbons variation diagram in the Shengli Oil Field soil that Fig. 2 provides for the embodiment of the present invention.
Detailed description of the invention
Technical scheme is further illustrated below by way of specific embodiment.
Embodiment 1 Liaohe Oil Field oil-polluted soils is repaired
Contaminated soil used by test picks up from well site, Liaohe Oil Field periphery (Liaoning Panjin).The soil adopted back going the removal of impurity, air-dries, levigate mistake 20 mesh sieve is standby.In soil, total content of organic carbon is 5.21%, and total petroleum hydrocarbons content is 3.45%, and total petroleum hydrocarbons content/total content of organic carbon is 0.66.
Before carrying out microorganism-electronic two-part and repairing, being uniformly blended into the fertilizer that organic carbon content is high in contaminated soil, in soil, total petroleum hydrocarbons content/total content of organic carbon is 0.45 after measured.The contaminated soil prepared is loaded long 30cm, wide 24cm, in the soil chamber of high 15cm.
The present embodiment sets 3 process, and the most independent microbial treatments, independent electrokinetic process and microorganism-electronic two stage process, actual conditions is shown in Table 1.Microbial treatments inoculate the most cultured petroleum hydrocarbon degradation bacterium, and inoculum concentration is 5.2 × 108Cfu/g dry ground.Electrokinetic process does not inoculate petroleum hydrocarbon degradation bacterium, but applies DC electric field, and electric field intensity is 1.2V/cm, uses the method for operation of polarity of electrode switching, and the polarity switching interval time is 8min.Microorganism-electronic two stage process first inoculates the after-applied DC electric field of petroleum hydrocarbon degradation bacterium, and petroleum hydrocarbon degradation bacterium inoculum concentration is 5.2 × 108Cfu/g dry ground, electric field intensity is 1.2V/cm, and electric field uses the method for operation of polarity switching, and the polarity switching interval time is 8min；Microorganism-electronic two stage process convergence condition is that now petroleum hydrocarbon removal rate slows down when total petroleum hydrocarbons content/total content of organic carbon >=0.6 in soil, uses the mode applying DC electric field to continue repair process.
Electrode used by independent electrokinetic process and the electronic Combined Treatment of microorganism is stainless steel electrode, and electrode diameter is 10mm, and electrode length is 120mm；All process that to keep the soil moistures be 25 DEG C, humidity is 25%.In processing procedure, sample once every 7d, total petroleum hydrocarbons content and total content of organic carbon change in monitoring soil.
Be can be seen that as microbial treatments soil Petroleum Hydrocarbon content/total content of organic carbon >=0.6 (28d) by measurement result (Fig. 1), the removal rate of petroleum hydrocarbon is reduced by microorganism, show the reduced capability of microbial degradation petroleum hydrocarbon, and after applying DC electric field at this moment, the clearance of petroleum hydrocarbon increases rapidly, when 77d, removal rate of petroleum hydrocarbons reaches 83.7%, and more single microbial treatments or electrokinetic process improve 30.6%, 38.8%.It can be said that bright, at the inefficient timing node of microbial treatments, apply electric field and can be effectively improved the removal efficiency of petroleum hydrocarbon, shortening processes the time.
Embodiment 2 Shengli Oil Field oil-polluted soils is repaired
Contaminated soil used by test picks up from Shengli Oil Field well site periphery (Shandong region).The soil adopted back going the removal of impurity, air-dries, levigate mistake 20 mesh sieve is standby.In soil, total content of organic carbon is 6.71%, and total petroleum hydrocarbons content is 2.82%, and total petroleum hydrocarbons content/total content of organic carbon is 0.42.
The present embodiment sets 3 process, and the most independent microbial treatments, independent electrokinetic process and microorganism-electronic two stage process, actual conditions is shown in Table 2.Microbial treatments inoculate the most cultured petroleum hydrocarbon degradation bacterium, and inoculum concentration is 6.8 × 108Cfu/g dry ground.Electrokinetic process does not inoculate petroleum hydrocarbon degradation bacterium, but applies DC electric field, and electric field intensity is 1.5V/cm, uses the method for operation of polarity of electrode switching, and the polarity switching interval time is 5min.Microorganism-electronic two stage process first inoculates the after-applied DC electric field of petroleum hydrocarbon degradation bacterium, and petroleum hydrocarbon degradation bacterium inoculum concentration is 6.8 × 108Cfu/g dry ground, electric field intensity is 1.5V/cm, and electric field uses the method for operation of polarity switching, and the polarity switching interval time is 5min；Microorganism-electronic two stage process convergence condition is that now petroleum hydrocarbon removal rate slows down when total petroleum hydrocarbons content/total content of organic carbon >=0.6 in soil, uses the mode applying DC electric field to continue repair process.
Electrode used by independent electrokinetic process and the electronic Combined Treatment of microorganism is stainless steel electrode, and electrode diameter is 10mm, and electrode length is 120mm；All process that to keep the soil moistures be 25 DEG C, humidity is 25%.In processing procedure, sample once every 7d, total petroleum hydrocarbons content and total content of organic carbon change in monitoring soil.
Be can be seen that as total petroleum hydrocarbons content/total content of organic carbon >=0.6 (21d) in microbial treatments soil by measurement result (Fig. 2), the removal rate of petroleum hydrocarbon is reduced by microorganism, show the reduced capability of microbial degradation petroleum hydrocarbon, and after applying DC electric field at this moment, the clearance of petroleum hydrocarbon increases rapidly, when 70d, removal rate of petroleum hydrocarbons reaches 71.6%, and more single microbial treatments or electrokinetic process improve 28.4%, 40.8%.It can be said that bright, at the inefficient timing node of microbial treatments, apply electric field and can be effectively improved the removal efficiency of petroleum hydrocarbon, shortening processes the time.
Table 1
Table 2
Claims (4)
1. the microorganism of an oil-polluted soils-electronic two-part restorative procedure, it is characterised in that: with
Total petroleum hydrocarbons content and the ratio of total content of organic carbon in contaminated soil, carry out microorganism remediation and electronic
Repair the conversion of two-part, and then reach the purpose of remedying oil-polluted soils；
The transfer point of described microorganism remediation and electro reclamation two-part is that in contaminated soil, total petroleum hydrocarbon contains
The ratio of amount and total content of organic carbon is 0.6；
In soil, total petroleum hydrocarbons content/total content of organic carbon < when 0.6, uses at the mode of microorganism remediation
Reason contaminated soil；
In soil during total petroleum hydrocarbons content/total content of organic carbon >=0.6, use at the mode of electro reclamation
Reason contaminated soil.
2. the microorganism of oil-polluted soils as described in claim 1-electronic two-part restorative procedure, its
Being characterised by: during described microorganism remediation, degradation bacteria be inoculated in contaminated soil, temperature is
15-30 DEG C, humidity be 15-35%, pH be 6.0-8.0, repair；Wherein, the inoculation of degradation bacteria
Amount is 5-8 × 108Cfu/g dry ground；
Described degradation bacteria is Bacillus licheniformis (Bacillus licheniformis), culture presevation number
For CCTCC NO:2011261, preservation mechanism is China typical culture collection center.
3. the microorganism of oil-polluted soils as described in claim 1-electronic two-part restorative procedure, its
It is characterised by: described organic carbon refers to humic acid or fertilizer.
4. the microorganism of oil-polluted soils as described in claim 1-electronic two-part restorative procedure, its
Being characterised by: during described electro reclamation, soil is applied unidirectional current, and the voltage gradient of soil is
1-1.5V/cm, uses the method for operation of polarity of electrode switching, and the polarity switching interval time is 5-10min,
Holding soil moisture be 15-35%, pH be 5.5-8.5.
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| CN104998895B (en) * | 2015-06-26 | 2018-03-23 | 西安工程大学 | Based on Plesiomonas, dynamic glue bacterium and the united petroleum-contaminated soil biodegrading process of electric field |
| CN104923559B (en) * | 2015-06-26 | 2018-03-23 | 西安工程大学 | Based on pseudomonad, dynamic glue bacterium and the united petroleum-contaminated soil biodegrading process of electric field |
| CN105396871B (en) * | 2015-12-21 | 2018-10-16 | 中国科学院沈阳应用生态研究所 | A kind of method that hydrocarbon contamination soil is repaired in efficient collaboration |
| CN105417909B (en) * | 2015-12-30 | 2018-10-09 | 哈尔滨工业大学 | A kind of oily sludge electrochemica biological coupling depth processing method |
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| CN108004163A (en) * | 2017-12-04 | 2018-05-08 | 中国科学院沈阳应用生态研究所 | Mix bacterium agent and its preparation and the application of petroleum hydrocarbon contaminated soil are repaired suitable for depth in medium temperature aerobe shut-down system |
| CN111440741B (en) * | 2020-04-03 | 2022-05-31 | 中国科学院沈阳应用生态研究所 | Functional bacterium suitable for restoring petroleum-polluted soil in direct-current electric field anode region and application |
| CN112588814B (en) * | 2020-12-24 | 2022-08-19 | 中国科学院沈阳应用生态研究所 | Electric-microorganism combined in-situ remediation method for contaminated soil |
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| CN101767105A (en) * | 2008-12-30 | 2010-07-07 | 中国科学院沈阳应用生态研究所 | Organic pollution soil repair system and method |
| CN101947539B (en) * | 2010-08-06 | 2012-02-22 | 浙江博世华环保科技有限公司 | Soil remediation method for treating heavy metal pollutants |
| CN103160448B (en) * | 2011-12-13 | 2014-06-11 | 中国科学院沈阳应用生态研究所 | Petroleum hydrocarbon degradation bacteria being suitable for electric field condition and application thereof |
| CN104056857B (en) * | 2013-03-22 | 2017-10-27 | 东北林业大学 | A kind of electronic auxiliary repairing method of microorganism of soil contaminated by crude oil |
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