CN104584897A - New purpose of ascorbic acid - Google Patents
New purpose of ascorbic acid Download PDFInfo
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- CN104584897A CN104584897A CN201410839539.6A CN201410839539A CN104584897A CN 104584897 A CN104584897 A CN 104584897A CN 201410839539 A CN201410839539 A CN 201410839539A CN 104584897 A CN104584897 A CN 104584897A
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- Prior art keywords
- formaldehyde
- ascorbic acid
- plant
- broad bean
- leaves
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- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 title claims abstract description 140
- 229960005070 ascorbic acid Drugs 0.000 title claims abstract description 70
- 235000010323 ascorbic acid Nutrition 0.000 title claims abstract description 61
- 239000011668 ascorbic acid Substances 0.000 title claims abstract description 61
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims abstract description 183
- 238000000746 purification Methods 0.000 claims description 14
- 240000006677 Vicia faba Species 0.000 abstract description 43
- 235000010749 Vicia faba Nutrition 0.000 abstract description 41
- 241000196324 Embryophyta Species 0.000 abstract description 34
- 235000002098 Vicia faba var. major Nutrition 0.000 abstract description 33
- 238000000034 method Methods 0.000 abstract description 20
- 238000005507 spraying Methods 0.000 abstract description 17
- 230000000694 effects Effects 0.000 abstract description 14
- 230000008569 process Effects 0.000 abstract description 13
- 238000010521 absorption reaction Methods 0.000 abstract description 6
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 abstract description 6
- 238000009825 accumulation Methods 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 abstract description 2
- 230000002349 favourable effect Effects 0.000 abstract 1
- 239000007921 spray Substances 0.000 description 21
- 244000046052 Phaseolus vulgaris Species 0.000 description 19
- 239000003153 chemical reaction reagent Substances 0.000 description 10
- 239000007789 gas Substances 0.000 description 10
- 239000002211 L-ascorbic acid Substances 0.000 description 9
- 235000000069 L-ascorbic acid Nutrition 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 9
- 230000008859 change Effects 0.000 description 9
- 239000012530 fluid Substances 0.000 description 9
- 238000010306 acid treatment Methods 0.000 description 8
- 239000003795 chemical substances by application Substances 0.000 description 8
- 210000002615 epidermis Anatomy 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- YRKCREAYFQTBPV-UHFFFAOYSA-N acetylacetone Chemical compound CC(=O)CC(C)=O YRKCREAYFQTBPV-UHFFFAOYSA-N 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 230000003750 conditioning effect Effects 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 238000011084 recovery Methods 0.000 description 4
- 238000003915 air pollution Methods 0.000 description 3
- 210000000170 cell membrane Anatomy 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- IEQAICDLOKRSRL-UHFFFAOYSA-N 2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-(2-dodecoxyethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethanol Chemical compound CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO IEQAICDLOKRSRL-UHFFFAOYSA-N 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000012531 culture fluid Substances 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 239000003599 detergent Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- ORZHVTYKPFFVMG-UHFFFAOYSA-N xylenol orange Chemical compound OC(=O)CN(CC(O)=O)CC1=C(O)C(C)=CC(C2(C3=CC=CC=C3S(=O)(=O)O2)C=2C=C(CN(CC(O)=O)CC(O)=O)C(O)=C(C)C=2)=C1 ORZHVTYKPFFVMG-UHFFFAOYSA-N 0.000 description 2
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 1
- 239000005695 Ammonium acetate Substances 0.000 description 1
- 238000009010 Bradford assay Methods 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 206010053615 Thermal burn Diseases 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 241000219873 Vicia Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 229960000583 acetic acid Drugs 0.000 description 1
- DPKHZNPWBDQZCN-UHFFFAOYSA-N acridine orange free base Chemical compound C1=CC(N(C)C)=CC2=NC3=CC(N(C)C)=CC=C3C=C21 DPKHZNPWBDQZCN-UHFFFAOYSA-N 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 229940043376 ammonium acetate Drugs 0.000 description 1
- 235000019257 ammonium acetate Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- DZBUGLKDJFMEHC-UHFFFAOYSA-N benzoquinolinylidene Natural products C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 1
- FFBHFFJDDLITSX-UHFFFAOYSA-N benzyl N-[2-hydroxy-4-(3-oxomorpholin-4-yl)phenyl]carbamate Chemical compound OC1=C(NC(=O)OCC2=CC=CC=C2)C=CC(=C1)N1CCOCC1=O FFBHFFJDDLITSX-UHFFFAOYSA-N 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 238000007084 catalytic combustion reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 238000006385 ozonation reaction Methods 0.000 description 1
- 235000019362 perlite Nutrition 0.000 description 1
- 239000010451 perlite Substances 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 238000013033 photocatalytic degradation reaction Methods 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 239000011044 quartzite Substances 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000005067 remediation Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000000779 smoke Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000005418 vegetable material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
Abstract
The invention discloses application of ascorbic acid to improvement of plant capability for purifying air formaldehyde pollution. In the use process, an ascorbic acid solution is used for spraying lower skin of potted broad bean leaves, after 8 hours, broad bean plants are respectively put into air environments containing 14.8mg/m<3> and 4mg/m<3> formaldehyde, and after the treatment for 1h in the 14.8mg/m<3> formaldehyde stress environment and the treatment for 48h in the 4mg/m<3> formaldehyde stress environment, the leaf physiological and biochemical index test is respectively carried out; experiment results show that compared with control plants, the plants sprayed with the ascorbic acid have the effects that the H2O2 accumulation in the broad bean leaves is reduced, the H<+>-pump activity and the stomatal aperture are respectively improved, the changes are favorable for improving the plant stomatal opening activity, and the absorption rate of the leaves on the formaldehyde is improved, so that the plant capability for purifying air formaldehyde pollution is enhanced. The results show that when the ascorbic acid at the proper concentration is used for spraying the plant leaves, the plant capability for purifying air formaldehyde pollution can be improved.
Description
Technical field
The invention belongs to plant purification air-polluting field, relate to the novelty teabag of ascorbic acid, be specially ascorbic acid and improving the application in plant purification air formaldehyde pollution capacity.
Background technology
Room air pollution become after " coal-smoke pollution " and " pollution of photochemical fog type " the 3rd pollute period.In the organic pollutant that indoor are numerous, formaldehyde, with its wide material sources, the feature such as harmfulness is large, the duration is long, becomes ubiquity and one of comparatively serious indoor pollutant.Correlation study shows that the concentration of China's indoor formaldehyde is generally higher than Indoor Air Quality standards, Formaldehyde Pollution of Indoor Air has become the topmost indoor air environment problem of China, seriously endanger the life and health of people, the formaldehyde pollution that purifies the air of a room has become the problem that people pay close attention to always.Current purifying formaldehyde mainly contains the methods such as absorption, condensation, Ozonation, photocatalytic degradation method, catalytic combustion, microbial cleaning, but the shortcomings such as these method existence conditions are harsh, expensive, complex operation, purification efficiency are low, secondary pollution, utilize plant purification Formaldehyde Pollution of Indoor Air to be a kind of economical and effective, and meet the pollution remediation technology of public's needs and psychology.Plant purification gas formaldehyde is mainly absorbed by pore, and the function of stomatal opening is strong and weak to be determined by stomatal aperture and degree of leading, and many biologies and abiotic factor can suppress aperture and the degree of leading of pore, thus have impact on plant absorption and the function of purifying formaldehyde.Therefore, a kind of efficient, easy, cheap and easy received plant stomata conditioning agent is found to have great reality and application value.
Broad bean (Vicia faba L.) belongs to a cultivar in Papilionaceae (Papi-lionaceae) Vetch, and annual or more year life, extensively plants in China.Broad bean lower epidermis stoma number is many, and pore is comparatively large, is the extraordinary vegetable material absorbing air formaldehyde.Find a kind of inexpensive and plant that function of absorbing formaldehyde is strong, excavate and a kind ofly can improve the purify air stomatal conductivity agent of formaldehyde pollution ability of broad bean there is important environment protection significance.
Summary of the invention
The object of this invention is to provide the novelty teabag of a kind of ascorbic acid (ASA), namely it is as the stomatal conductivity agent improving plant purification air formaldehyde pollution capacity, and namely ascorbic acid is improving the effect in plant purification formaldehyde pollution.
In order to realize above-mentioned purpose of the present invention, technical scheme of the present invention is as follows:
(1) the autumn sowing broad bean full seed in Yunnan is selected to carry out boiling hot kind, scald dead germ and worm's ovum, Germination under 25 DEG C of dark, treat that root grows 2cm and proceeds to flowerpot cultivation, three healthy and strong seeds sprouted are placed in each flowerpot, within between culture period two days, water a water, grow after-applied Hoagland ' the s culture fluid of leaf;
(2) when broad bean seedling grows to four pairs of blades, select the healthy and strong plant that growing way is consistent, with 0.1,0.5,2,5,10 mmol/L ascorbic acid solutions, potted plant Broad Bean Leaves lower epidermis is sprayed respectively, put into respectively containing 14.8 mg/m after 8 hours
3and 4mg/m
3in the air ambient of gas formaldehyde, at 14.8 mg/m
31h and 4mg/m is processed in formaldehyde stress environment
3process 48h in formaldehyde stress environment, each process arranges three repetitions, gets the mensuration that second pair of several from top to bottom leaf of broad bean seedling carries out physiological and biochemical index, therefrom filters out best spraying concentration;
(3) respectively with the ASA process broad bean of best spraying concentration, process according to the above-mentioned processing time, and get the mensuration of second pair of leaf for physiological and biochemical index.
The stomatal conductivity agent that ascorbic acid provided by the invention pollutes as plant purification air formaldehyde, easy to use, cost is very low.This conditioning agent significantly improves the ability that plant purification air formaldehyde is polluted; open the new way improving plant purification formaldehyde pollution with conditioning agent; contribute to the research that scientific worker's Ascorbic Acid improves plant purification air formaldehyde pollution capacity molecule mechanism; have broad prospects in the room air pollution prevention and control field of environmental protection, also open economical crops plays new role blank in removing pollutant field.
Beneficial effect of the present invention: the conditioning agent of raising plant purification air formaldehyde pollution capacity of the present invention, has and drops into the feature low, simple to operate, efficiency is high.Under normal temperature, ascorbic acid is the stomatal conductivity agent that more satisfactory plant purification air formaldehyde is polluted, and ascorbic acid sprays and can reduce H in blade cell
2o
2accumulation, improves H on cell membrane
+-pump is active, improves stomatal aperture, thus improves formaldehyde absorption rate, significant to room air pollution control.
Accompanying drawing explanation
Fig. 1 is that variable concentrations ascorbic acid sprays rear Broad Bean Leaves H
2o
2assay result; In figure, N represents in gas-tight silo the bean plant not having formaldehyde stress also not spray ascorbic acid treatment fluid; 0 to represent in gas-tight silo by formaldehyde stress but does not spray the bean plant of ascorbic acid treatment fluid; 0.1-10 mmol/L represents in gas-tight silo by 14.8 mg/m
3gas formaldehyde is coerced but has been sprayed the bean plant of variable concentrations ascorbic acid;
Fig. 2 is that variable concentrations ascorbic acid sprays rear Broad Bean Leaves H
+-pump determination of activity result; In figure, N represents in gas-tight silo the bean plant not having formaldehyde stress also not spray ascorbic acid treatment fluid; 0 to represent in gas-tight silo by formaldehyde stress but does not spray the bean plant of ascorbic acid treatment fluid; 0.1-10 mmol/L represents in gas-tight silo by 14.8 mg/m
3gas formaldehyde is coerced but has been sprayed the bean plant of variable concentrations ascorbic acid;
Fig. 3 is the change that variable concentrations ascorbic acid sprays rear Broad Bean Leaves stomatal aperture;
Fig. 4 is that variable concentrations ascorbic acid sprays residual formaldehyde change in concentration in rear gas-tight silo;
Fig. 5 is the change that variable concentrations ascorbic acid sprays Leaf Cells of Vicia Faba Free-formaldehyde concentration in rear gas-tight silo;
Fig. 6 be 2 mmol/L ascorbic acid spray rear bean plant in cabinet and cabinet process the blade H that 48h records outward
2o
2changes of contents;
Fig. 7 be 2 mmol/L ascorbic acid spray rear bean plant in cabinet and cabinet process the blade plasma membrane H that 48h records outward
+-pump activity change;
Fig. 8 be 2 mmol/L ascorbic acid spray rear bean plant in cabinet and cabinet process the Stoma of Leaves aperture change that 48h records outward;
Fig. 9 be 2 mmol/L ascorbic acid spray rear bean plant in cabinet and cabinet process the leaf stomatal conductance change that 48h records outward;
In above-mentioned figure, ASA is ascorbic acid.
Embodiment
Below by embodiment and accompanying drawing, the present invention is described in further detail, but scope is not limited to described content.In embodiment, method if no special instructions, and operation is carried out routinely, and use reagent is the reagent that routine is purchased reagent or prepared according to a conventional method if no special instructions.
Embodiment 1: the cultivation of bean plant and process, step is as follows:
1, experiment material is Vicia faba seedling
Be seeded in the flowerpot that red soil and perlite mix with mass ratio 6:1 ratio after Broad Bean Seeds vernalization, three healthy and strong seeds sprouted are placed in each flowerpot, within between culture period two days, water a water, grow after-applied Hoagland ' the s culture fluid of leaf, test for this when seedling grows to four pairs of leaves;
2, the ascorbic acid treatment fluid of variable concentrations (0.1,0.5,2,5,10 mmol/L) is configured;
3, spray Broad Bean Leaves lower epidermis with the treatment fluid of above-mentioned concentration gradient respectively, be covered with the globule with lower epidermis and the amount of spraying of not dripping as often opening blade, the bean plant sprayed with water is blank.After 8 hours ascorbic acid treatment fluid processing times, bean plant is put into air containing 14.8 mg/m
3in the glass capsulation storehouse of gas formaldehyde, this device specification is that 700 mm × 700, mm × 600 mm(are long × wide × high), after testing without gas leak phenomenon.The both sides of gas-tight silo provide light source, and in storehouse, intensity of illumination is 600 μm of ol/m
2s, the corner of gas-tight silo is equipped with four small fans, is dispersed evenly to space in storehouse to accelerate gas formaldehyde at storehouse Inner eycle.Temperature and humidity in gas-tight silo is by sensor (CH
2o/C-10, MEMBRAPOR, Swizerland) detect and automatically show at instrument board.Formaldehyde stress processing method is as follows:
Drip mass percent concentration be the formalin of 37% on little sponge ball, hung on by sponge ball in gas-tight silo, formaldehyde volatilizees from sponge ball, the concentration of formaldehyde volatilized by above measuring chamber display screen show reading, at concentration of formaldehyde close to 14.8 mg/m
3time bean plant is put into gas-tight silo, seal door, when gas-tight silo initial concentration reaches above-mentioned concentration, sponge ball taken out.After broad bean Stress treatment 1h, in storehouse, concentration of formaldehyde starts to decline, and the mensuration of every physiological and biochemical index is carried out in sampling, and the bean plant sprayed with water is contrast, and each process arranges three repetitions.Experimental session round the clock temperature Change at 13-22 DEG C, absorbs formaldehyde for avoiding soil and edaphon and impacts experimental result, with the sealing of PV film bottom from plant base portion to flowerpot.
Embodiment 2: adopt the Broad Bean Leaves in embodiment 1 after the 3rd step process to carry out H
2o
2content and H
+the determination of activity of-pump
1, H
2o
2assay: adopt xylenol orange method, take fresh plant blade, add after pre-cold acetone becomes homogenate with a little quartzite sand grind according to material and the ratio of extractant mass volume ratio 1:3, proceed to centrifuge tube in 12000g, centrifugal 20min at 4 DEG C, discard residue, supernatant is sample extracting solution.Use ddH respectively
2o reagent preparation A (includes 3.3 mmol/L FeSO
4, 3.3 mmol/L (NH
4)
2sO
4, 412.5 mmol/L H
2sO
4) and reagent B (including 165 μm of ol/L xylenol orange, 165 mmol/L sorbierites), use front reagent A and reagent B to form working reagent according to the ratio mixing of volume ratio 1:10.This working reagent and H
2o
2liquid to be measured mixes according to the ratio of volume ratio 2:1, and 30 DEG C of water-bath colour developing 30min, measure OD value in 560nm place, calculate H
2o
2content (Fig. 1).
2, plasmalemma protein extracts and concentration determination: the Broad Bean Leaves Extraction of plasma membrane that different ascorbic acid concentrations sprays uses the kit of Bei Bo reagent Co., Ltd to carry out.Plasmalemma protein after extraction Bradford method measures plasmalemma protein concentration, at the ddH of 800 μ L
2add the plasmalemma protein of 5 μ L in O, mixing, then adds the Bradford solution of 200 μ L, and room temperature leaves standstill 10 minutes, measures OD
595, calculate the volume that 50 μ g plasmalemma proteins are corresponding.
3, H
+-pump determination of activity step is as follows:
Use BTP to adjust pH to 6.0 containing 5 mmol/L BTP/MES (pH 6.0), 12 μm of ol/L AO, 300 mmol/L KCl, 250 mmol/L sucrose, 0.5 mmol/L EGTA(in (1) 1.5 ml reaction system), 1 mmol/L NaN
3, 1 mmol/L Na
2moO
4, 50 mmol/L KNO
30.05% Brij-35 (w/v) and 100 μ g plasmalemma proteins, adding abstergent Brij-35 makes original position film overturn, and after reaction mixture at room temperature places 20 min, adds 5 mmol/L ATP-BTP(pH=6.0) mixed liquor to be to start reaction;
(2) with reactant liquor zeroing contrast, record per minute OD value, mensuration acridine orange is 8 minutes internal absorbance quencher speed at 492 nm place, reflects that the Leaf Cells of Vicia Faba membrane vesicle body of different ascorbic acid spraying concentration pumps H by quencher speed
+ability, i.e. H
+-pump activity (Fig. 2).
As can be seen from Figure 1, after spraying the ascorbic acid of variable concentrations, the H of Broad Bean Leaves
2o
2content compares with plant in the cabinet not spraying ascorbic acid the minimizing all had in various degree; 2 mmol/L ascorbic acid processed group H
2o
2content reduces at most, even lower than the outer plant of the cabinet not spraying ascorbic acid; Ascorbic acid concentrations raises again, H
2o
2content no longer includes significant change.As can be seen from Figure 2, after spraying the ascorbic acid of variable concentrations, the H of Broad Bean Leaves
+-pump compares with plant in the cabinet not spraying ascorbic acid the recovery had in various degree, 2 mmol/L ASA processed group H
+-pump activation recovering is the strongest.H
2o
2and H
+-pump is the important regulatory factor in the open activity of plant stomata, H
2o
2content reduces and H
+-pump increased activity all contributes to the increase of stomatal aperture or degree of leading, thus promotes stomatal opening.
Embodiment 3: Stoma of Leaves aperture measures
Processed the blade of 1 hour in Example 1 the 3rd step, torn and take off epidermis, be placed on cover glass, dripped a physiological saline, covered, in times Microscopic observation measure stomatal aperture, each concentration random measurement 40, average (Fig. 3).
From Fig. 3, variable concentrations ascorbic acid sprays rear Broad Bean Leaves stomatal aperture recovery in various degree, but the recovery extent of 2 mmol/L ASA to Broad Bean Leaves stomatal aperture is maximum.
Embodiment 4: process 1 hour in embodiment 1 the 3rd step after, start to record residual formaldehyde concentration in gas-tight silo air, 10 minutes records once, record 100 minutes (Fig. 4) continuously.And the Leaf Cells of Vicia Faba Free-formaldehyde content having sprayed variable concentrations ascorbic acid in gas-tight silo is measured.Assay method is as follows:
Get 1.0g Broad Bean Leaves and add the homogenate of 1mL Tris extract liquid nitrogen grinding, proceed to centrifuge tube in 12000g, centrifugal 20min at 4 DEG C, discard residue, supernatant adds 1 mL Nash reagent (acetylacetone,2,4-pentanedione: 0.2%, glacial acetic acid: 0.3%, ammonium acetate: 15%), 30 DEG C of insulations measure OD in 30 minutes afterwards
410, the concentration of blade Free-formaldehyde is calculated according to calibration curve.
From Fig. 5, after formaldehyde stress 1h, place sprayed ascorbic acid bean plant gas-tight silo in air formaldehyde decrease speed than place do not spray ascorbic acid bean plant gas-tight silo in fast, the broad bean wherein having sprayed 2 mmol/L ascorbic acid makes air formaldehyde in gas-tight silo reduce more.Fig. 5 demonstrates air formaldehyde in gas-tight silo and reduces relevant with the formaldehyde amount that Broad Bean Leaves absorbs, the formaldehyde that the Broad Bean Leaves having sprayed 2 mmol/L ascorbic acid absorbs is maximum, and this illustrates that the ability effect that the ascorbic acid of this concentration improves absorption formaldehyde to Broad Bean Leaves is maximum.
Embodiment 5: according to above-mentioned Leaf Cells of Vicia Faba H
2o
2content, stomatal aperture, cytoplasma membrane H
+the assay result of residual formaldehyde concentration and blade cell Free-formaldehyde in-pump activity, gas-tight silo, filtering out 2 mmol/L is the optium concentration that ascorbic acid sprays.In order to verify the facilitation of 2 mmol/L ascorbic acid detergent power that gas formaldehyde pollutes in plant is to true indoor environment, Broad Bean Leaves lower epidermis is sprayed with the ascorbic acid treatment fluid of 2 mmol/L, the globule is covered with and the amount of spraying of not dripping as often opening blade with lower epidermis, after 8 hours ascorbic acid treatment fluid processing times, broad bean being put into release gas formaldehyde daily mean of concentration is 4mg/m
3timeworn furniture cabinet in, furniture cabinet three is made up of glass door compressed sheet (59 ' 40 ' 48cm), one side, the flowerpot of bean plant is had to put into cabinet rear enclosed cabinet door by long, Stress treatment 48h, respectively in outer, the cabinet of sampling and measuring cabinet (not spraying ascorbic acid) and cabinet interior+the plant leaf cell of ascorbic acid (spraying ascorbic acid in cabinet) in ascorbic acid content and H
+-pump is active.Each process arranges three repetitions, sprays blade lower epidermis and to be placed in cabinet and bean plant outside cabinet is contrast with water.The conditions such as the illumination in switch board and outside cabinet, temperature and relative moisture are in close level, and illumination and dark processing time are set as 12h/12h, experimental session round the clock temperature Change and flowerpot encapsulating method the same.
Can see from Fig. 6,7, in formaldehyde pollution environment, the Broad Bean Leaves having sprayed ascorbic acid in cabinet is compared with the Broad Bean Leaves not spraying ascorbic acid, H
2o
2accumulation obviously declines (Fig. 6), H
+-pump is active obviously raises (Fig. 7).This illustrates that the ascorbic acid of 2 mmol/L consumes H in Leaf Cells of Vicia Faba
2o
2, and the H of Leaf Cells of Vicia Faba film under making formaldehyde stress
+-pump activity is gone up.
Embodiment 6: process with embodiment 5, measures the stomatal aperture and degree of leading (Fig. 8,9) that do not spray the Broad Bean Leaves spraying ascorbic acid in ascorbic acid and cabinet in 48h cabinet.
From Fig. 8 and Fig. 9, because gas formaldehyde is coerced, in cabinet, the stomatal aperture of Broad Bean Leaves all obviously declines with degree of leading, but after spraying ascorbic acid, is obtained for obvious recovery by Broad Bean Leaves stomatal aperture in the cabinet of formaldehyde stress and degree of leading.It can thus be appreciated that the ascorbic acid of 2 mmol/L contributes to the raising of plant leaf blade stomatal aperture and degree of leading in real formaldehyde pollution air ambient, thus promote plant absorption formaldehyde, purifying formaldehyde pollutes.
Claims (1)
1. ascorbic acid is improving the application in the pollution of plant purification air formaldehyde.
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| CN104871847A (en) * | 2015-05-14 | 2015-09-02 | 昆明理工大学 | Application of ascorbic acid in plant nitrate nitrogen adsorption under increased aluminum stress |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2859316Y (en) * | 2005-11-09 | 2007-01-17 | 周宏伟 | Plant-supported biological filtering air purifier |
-
2014
- 2014-12-30 CN CN201410839539.6A patent/CN104584897A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2859316Y (en) * | 2005-11-09 | 2007-01-17 | 周宏伟 | Plant-supported biological filtering air purifier |
Non-Patent Citations (5)
| Title |
|---|
| 刘亚丽，侯瑛: "抗坏血酸、甲醇对蚕豆气孔面积及生理指标的影响", 《农业科学》 * |
| 刘栋等: "甲醛气体胁迫对3种观赏植物的形态及部分生理指标的影响", 《河北农业大学学报》 * |
| 安国勇等: "过氧化氢对蚕豆气孔运动和质膜K+通道的影响", 《植物生理学报》 * |
| 陈坤明等: "植物抗坏血酸的生物合成、转运及其生物学功能", 《西北植物学报》 * |
| 鲁敏等: "室内植物受甲醛污染胁迫响应机制的研究进展", 《山东建筑大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104871847A (en) * | 2015-05-14 | 2015-09-02 | 昆明理工大学 | Application of ascorbic acid in plant nitrate nitrogen adsorption under increased aluminum stress |
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