CN104541662A - Application of sodium tungstate for improving formaldehyde pollution purification of pot plant - Google Patents
Application of sodium tungstate for improving formaldehyde pollution purification of pot plant Download PDFInfo
- Publication number
- CN104541662A CN104541662A CN201410839558.9A CN201410839558A CN104541662A CN 104541662 A CN104541662 A CN 104541662A CN 201410839558 A CN201410839558 A CN 201410839558A CN 104541662 A CN104541662 A CN 104541662A
- Authority
- CN
- China
- Prior art keywords
- plant
- formaldehyde
- sodium tungstate
- leaves
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 title claims abstract description 198
- XMVONEAAOPAGAO-UHFFFAOYSA-N sodium tungstate Chemical compound [Na+].[Na+].[O-][W]([O-])(=O)=O XMVONEAAOPAGAO-UHFFFAOYSA-N 0.000 title claims abstract description 54
- 238000000746 purification Methods 0.000 title abstract description 15
- 240000006677 Vicia faba Species 0.000 abstract description 45
- 235000010749 Vicia faba Nutrition 0.000 abstract description 43
- 241000196324 Embryophyta Species 0.000 abstract description 37
- 230000000694 effects Effects 0.000 abstract description 23
- 238000005507 spraying Methods 0.000 abstract description 18
- 210000002615 epidermis Anatomy 0.000 abstract description 9
- 238000010521 absorption reaction Methods 0.000 abstract description 6
- 238000009825 accumulation Methods 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 abstract description 3
- 235000002098 Vicia faba var. major Nutrition 0.000 description 33
- 238000000034 method Methods 0.000 description 19
- 239000007921 spray Substances 0.000 description 18
- 244000046052 Phaseolus vulgaris Species 0.000 description 17
- 239000011734 sodium Substances 0.000 description 14
- 230000008569 process Effects 0.000 description 12
- 239000003153 chemical reaction reagent Substances 0.000 description 10
- 210000004027 cell Anatomy 0.000 description 9
- 239000012530 fluid Substances 0.000 description 9
- 239000007789 gas Substances 0.000 description 9
- 239000003795 chemical substances by application Substances 0.000 description 8
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- 229910052708 sodium Inorganic materials 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 230000008859 change Effects 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 238000011084 recovery Methods 0.000 description 5
- YRKCREAYFQTBPV-UHFFFAOYSA-N acetylacetone Chemical compound CC(=O)CC(C)=O YRKCREAYFQTBPV-UHFFFAOYSA-N 0.000 description 4
- 230000003750 conditioning effect Effects 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- IEQAICDLOKRSRL-UHFFFAOYSA-N 2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-(2-dodecoxyethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethanol Chemical compound CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO IEQAICDLOKRSRL-UHFFFAOYSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N acetic acid Substances CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 238000003915 air pollution Methods 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 239000012531 culture fluid Substances 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 239000003599 detergent Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- ORZHVTYKPFFVMG-UHFFFAOYSA-N xylenol orange Chemical compound OC(=O)CN(CC(O)=O)CC1=C(O)C(C)=CC(C2(C3=CC=CC=C3S(=O)(=O)O2)C=2C=C(CN(CC(O)=O)CC(O)=O)C(O)=C(C)C=2)=C1 ORZHVTYKPFFVMG-UHFFFAOYSA-N 0.000 description 2
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 1
- 239000005695 Ammonium acetate Substances 0.000 description 1
- 238000009010 Bradford assay Methods 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 206010053615 Thermal burn Diseases 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 241000219873 Vicia Species 0.000 description 1
- 229960000583 acetic acid Drugs 0.000 description 1
- DPKHZNPWBDQZCN-UHFFFAOYSA-N acridine orange free base Chemical compound C1=CC(N(C)C)=CC2=NC3=CC(N(C)C)=CC=C3C=C21 DPKHZNPWBDQZCN-UHFFFAOYSA-N 0.000 description 1
- 229940043376 ammonium acetate Drugs 0.000 description 1
- 235000019257 ammonium acetate Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- DZBUGLKDJFMEHC-UHFFFAOYSA-N benzoquinolinylidene Natural products C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 1
- FFBHFFJDDLITSX-UHFFFAOYSA-N benzyl N-[2-hydroxy-4-(3-oxomorpholin-4-yl)phenyl]carbamate Chemical compound OC1=C(NC(=O)OCC2=CC=CC=C2)C=CC(=C1)N1CCOCC1=O FFBHFFJDDLITSX-UHFFFAOYSA-N 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000007084 catalytic combustion reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 238000006385 ozonation reaction Methods 0.000 description 1
- 235000019362 perlite Nutrition 0.000 description 1
- 239000010451 perlite Substances 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 238000013033 photocatalytic degradation reaction Methods 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 239000011044 quartzite Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000005067 remediation Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000000779 smoke Substances 0.000 description 1
- RMJPDRUNCDRUQC-MCDZGGTQSA-M sodium;[[[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl] hydrogen phosphate Chemical compound [Na+].C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)([O-])=O)[C@@H](O)[C@H]1O RMJPDRUNCDRUQC-MCDZGGTQSA-M 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000005418 vegetable material Substances 0.000 description 1
Landscapes
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
Abstract
The invention discloses an application of sodium tungstate for improving formaldehyde pollution purification of a pot plant. During use, a sodium tungstate solution is used for spraying the lower epidermis of leaves of a pot vicia faba, after 8 hours, the vicia faba plant is placed into air environments containing 14.8 mg/m<3> and 4 mg/m<3> of gas formaldehyde respectively, the plant is treated for 1 h in a formaldehyde stress environment with the concentration of 14.8 mg/m<3> and treated for 48 h in the formaldehyde stress environment with the concentration of 4 mg/m<3>, and then physiological and biochemical indexes of the leaves are determined respectively; experiment results show that compared with a control plant, H2O2 accumulation in leaf cells of vicia faba sprayed with sodium tungstate is reduced, H<+>- pump activity and stomatal aperture are increased, stomatal opening activity of the plant can be improved through the changes, the formaldehyde absorption rate of the leaves is increased, the capacity of the plant for purifying formaldehyde pollution in air is improved, and the capacity of the plant for purifying formaldehyde pollution in the air can be improved by spraying sodium tungstate with proper concentration to the plant leaves.
Description
Technical field
The invention belongs to plant purification air-polluting field, be specifically related to the novelty teabag of sodium tungstate in plant purification air formaldehyde is polluted.
Background technology
Room air pollution become after " coal-smoke pollution " and " pollution of photochemical fog type " the 3rd pollute period.In the organic pollutant that indoor are numerous, formaldehyde, with its wide material sources, the feature such as harmfulness is large, the duration is long, becomes ubiquity and one of comparatively serious indoor pollutant.Correlation study shows that the concentration of China's indoor formaldehyde is generally higher than Indoor Air Quality standards, Formaldehyde Pollution of Indoor Air has become the topmost indoor air environment problem of China, seriously endanger the life and health of people, the formaldehyde pollution that purifies the air of a room has become the problem that people pay close attention to always.Current purifying formaldehyde mainly contains the methods such as absorption, condensation, Ozonation, photocatalytic degradation method, catalytic combustion, microbial cleaning, but the shortcomings such as these method existence conditions are harsh, expensive, complex operation, purification efficiency are low, secondary pollution, utilize plant purification Formaldehyde Pollution of Indoor Air to be a kind of economical and effective, and meet the pollution remediation technology of public's needs and psychology.Plant purification gas formaldehyde is mainly absorbed by pore, and the function of stomatal opening is strong and weak to be determined by stomatal aperture and degree of leading, and many biologies and abiotic factor can suppress aperture and the degree of leading of pore, thus have impact on plant absorption and the function of purifying formaldehyde.Therefore, find a kind of efficient, easy, cheap and easy received plant stomata conditioning agent and the strong plant of function of absorbing formaldehyde significant.
Broad bean (Vicia faba L.) belongs to a cultivar in Papilionaceae (Papi-lionaceae) Vetch, and annual or more year life, extensively plants in China.Broad bean lower epidermis stoma number is many, and pore is comparatively large, is the extraordinary vegetable material absorbing air formaldehyde.Excavate a kind of broad bean that can improve to purify air the stomatal conductivity agent of formaldehyde pollution ability, find a kind of inexpensive and function of absorbing formaldehyde is strong plant and there is great reality and application value.
Summary of the invention
The object of the invention is to provide a kind of novelty teabag of sodium tungstate, and it is as the stomatal conductivity agent improving plant purification air formaldehyde pollution capacity, i.e. sodium tungstate (Na
2wO
42H
2o) effect in plant purification formaldehyde pollution.
In order to realize above-mentioned purpose of the present invention, technical scheme of the present invention is as follows:
(1) the autumn sowing broad bean full seed in Yunnan is selected to carry out boiling hot kind, scald dead germ and worm's ovum, Germination under 25 DEG C of dark, treat that root grows 2cm and proceeds to flowerpot cultivation, three healthy and strong seeds sprouted are placed in each flowerpot, within between culture period two days, water a water, grow after-applied Hoagland ' the s culture fluid of leaf;
(2) when broad bean seedling grows to four pairs of blades, the healthy and strong plant that growing way is consistent is selected, with 0.5,1.5,4.5 mmol/L sodium tungstates, potted plant Broad Bean Leaves lower epidermis is sprayed respectively, the air ambient containing formaldehyde is put into respectively, at 14.8 mg/m after 8 hours
31h and 4mg/m is processed in formaldehyde stress environment
3process 48h in formaldehyde stress environment, each process arranges three repetitions; Get the mensuration that second pair of several from top to bottom leaf of broad bean seedling carries out physiological and biochemical index, therefrom filter out best spraying concentration;
(3) respectively with the sodium tungstate process broad bean of best spraying concentration (1.5 mmol/L), process according to the above-mentioned processing time, and get the mensuration of second pair of leaf for physiological and biochemical index.
Sodium tungstate (Na provided by the invention
2wO
42H
2o) as the stomatal conductivity agent that plant purification air formaldehyde is polluted, easy to use, cost is very low.This conditioning agent significantly improves the ability that plant purification air formaldehyde is polluted; open the new way improving plant purification formaldehyde pollution with conditioning agent; contribute to scientific worker improves plant purification air formaldehyde pollution capacity molecule mechanism research to sodium tungstate; have broad prospects in the room air pollution prevention and control field of environmental protection, also open economical crops plays new role blank in removing pollutant field.
Beneficial effect of the present invention: the conditioning agent of raising plant purification air formaldehyde pollution capacity of the present invention, has and drops into the feature low, simple to operate, efficiency is high.Under normal temperature, sodium tungstate is the stomatal conductivity agent that more satisfactory plant purification air formaldehyde is polluted, and sodium tungstate is used and can be reduced H in blade cell
2o
2accumulation, improves H on cell membrane
+-pump is active, improves stomatal aperture, thus improves formaldehyde absorption rate, significant to room air pollution control.
Accompanying drawing explanation
Fig. 1 is that variable concentrations sodium tungstate sprays rear Broad Bean Leaves H
2o
2content (A figure), PM H
+-ATPase activity (B figure) measurement result; In figure, N represents in gas-tight silo does not have formaldehyde stress not spray Na yet
2wO
4the bean plant for the treatment of fluid; Be subject to formaldehyde stress in 0 expression gas-tight silo but do not spray Na
2wO
4the bean plant for the treatment of fluid; 0.5-4.5 mmol/L represents in gas-tight silo by 14.8 mg/m
3gas formaldehyde is coerced but has been sprayed variable concentrations Na
2wO
4bean plant;
Fig. 2 is that variable concentrations sodium tungstate sprays rear Broad Bean Leaves H
+-pump determination of activity result; In figure, N represents in gas-tight silo the bean plant not having formaldehyde stress also not spray sodium tungstate treatment fluid; 0 to represent in gas-tight silo by formaldehyde stress but does not spray the bean plant of sodium tungstate treatment fluid; 0.5-4.5 mmol/L represents in gas-tight silo by 14.8 mg/m
3gas formaldehyde is coerced but has been sprayed the bean plant of variable concentrations sodium tungstate;
Fig. 3 is the change that variable concentrations sodium tungstate sprays rear Broad Bean Leaves stomatal aperture;
Fig. 4 is the change that variable concentrations sodium tungstate sprays residual formaldehyde concentration in rear gas-tight silo (A figure) and Leaf Cells of Vicia Faba Free-formaldehyde concentration (B figure);
Fig. 5 is that 1.5 mmol/L sodium tungstates spray rear bean plant in furniture cabinet, process the blade H that 48h records
2o
2content (A figure), PM H
+-ATPase activity (B figure) measurement result;
Fig. 6 is that 1.5 mmol/L sodium tungstates spray rear bean plant in furniture cabinet, process the blade H that 48h records
+-pump activity (C) measurement result;
Fig. 7 is the change that 1.5 mmol/L sodium tungstates spray that rear bean plant processes Stoma of Leaves aperture (A figure) that 48h records and degree of leading (figure B) in furniture cabinet.
Embodiment
Below by embodiment and accompanying drawing, the present invention is described in further detail, but scope is not limited to described content.In embodiment, method if no special instructions, and operation is carried out routinely, and use reagent is the reagent that routine is purchased reagent or prepared according to a conventional method if no special instructions.
Embodiment 1: the cultivation of bean plant and process
1, experiment material is Vicia faba seedling, be seeded in the flowerpot that red soil and perlite mix with 6:1 ratio after Broad Bean Seeds vernalization, three healthy and strong seeds sprouted are placed in each flowerpot, within between culture period two days, water a water, grow after-applied Hoagland ' the s culture fluid of leaf, test for this when seedling grows to four pairs of leaves;
2, the sodium tungstate treatment fluid of variable concentrations (0.5,1.5,4.5 mmol/L) is configured;
3, spray Broad Bean Leaves lower epidermis with the treatment fluid of above-mentioned concentration gradient respectively, be covered with the globule with lower epidermis and the amount of spraying of not dripping as often opening blade, the bean plant sprayed with water is blank.After 8 hours sodium tungstate treatment fluid processing times, bean plant is put into air containing 14.8 mg/m
3in the glass capsulation storehouse of gas formaldehyde, this device specification is that 700 × 600 × 700 mm(are long × wide × high), after testing without gas leak phenomenon.The both sides of gas-tight silo provide light source, and in storehouse, intensity of illumination is 600 μm of ol/m
2s, the corner of gas-tight silo is equipped with four small fans, is dispersed evenly to space in storehouse to accelerate gas formaldehyde at storehouse Inner eycle.Temperature and humidity in gas-tight silo is by sensor (CH
2o/C-10, MEMBRAPOR, Swizerland) detect and automatically show at instrument board.In Stress treatment process, in storehouse, average epidemic disaster that is initial and end eventually is respectively 30 ± 1.91 DEG C, 30 ± 0.33% and 32 ± 0.79 DEG C, 75 ± 0.85%.Formaldehyde stress processing method is as follows: drip 37% formalin on little sponge ball, hung on by sponge ball in gas-tight silo, formaldehyde volatilizees from sponge ball, and the concentration of formaldehyde volatilized shows reading, at concentration of formaldehyde close to 14.8 mg/m by the display screen above measuring chamber
3time bean plant is put into gas-tight silo, seal door, when gas-tight silo initial concentration reaches above-mentioned concentration, sponge ball taken out.After broad bean Stress treatment 1h, in storehouse, concentration of formaldehyde starts to decline, and the mensuration of every physiological and biochemical index is carried out in sampling, and the bean plant sprayed with water is contrast, and each process arranges three repetitions.Experimental session round the clock temperature Change at 13-22 DEG C, absorbs formaldehyde for avoiding soil and edaphon and impacts experimental result, with the sealing of PV film bottom from plant base portion to flowerpot.
Embodiment 2: adopt the Broad Bean Leaves in embodiment 1 after the 3rd step process to carry out H
2o
2content and H
+the determination of activity of-pump
1, H
2o
2assay:
Adopt xylenol orange method.Take fresh plant blade, add after pre-cold acetone becomes homogenate with a little quartzite sand grind, proceed to centrifuge tube according to material and the ratio of extractant mass volume ratio 3:1,12000g, centrifugal 20min at 4 DEG C, discard residue, supernatant is sample extracting solution.Use ddH respectively
2o reagent preparation A (includes 3.3 mmol/L FeSO
4, 3.3 mmol/L (NH
4) 2SO
4, 412.5 mmol/L H
2sO
4) and reagent B (including 165 μm of ol/L xylenol orange, 165 mmol/L sorbierites), use front reagent A and reagent B to form working reagent according to the ratio mixing of 1:10.This working reagent and H
2o
2liquid to be measured mixes according to the ratio of volume ratio 2:1, and 30 DEG C of water-bath colour developing 30min, measure OD value in 560nm place, calculate H
2o
2content (Figure 1A).
2, plasmalemma protein extracts and concentration determination: the Broad Bean Leaves Extraction of plasma membrane that different concentration of sodium tungstate sprays uses the kit of Bei Bo reagent Co., Ltd to carry out.Plasmalemma protein after extraction Bradford method measures plasmalemma protein concentration, at the ddH of 800 μ L
2add the plasmalemma protein of 5 μ L in O, mixing, then adds the Bradford solution of 200 μ L, and room temperature leaves standstill 10 minutes, surveys OD
595, calculate the volume that 50 μ g plasmalemma proteins are corresponding.
3, PM H
+-ATPase determination of activity step is as follows:
(1) PM H
+being determined at of-ATPase activity is carried out in the reaction system of 0.5 mL; Reaction system comprises 50 mmol/L BTP/MES, 5 mmol/L MgSO
4, 50 mmol/L KCl, 0.02% Brij-35 (w/v), 50 mmol/L KNO
3, 1 mmol/L (NH
4)
2moO
4, 1 mmol/L NaN
3, 4 mmol/L ATP-Na
2, after adding the plasmalemma protein extract of 50 μ g, start reaction;
(2) reactant mixture to be placed in after 37 DEG C of water-bath 30min ice bath immediately, to add reaction terminating liquid 1ml 2% H
2sO
4(v/v), 5% SDS (w/v) and 0.7 % (NH
4)
2moO
4(w/v) after, add 50 μ L 10% Vc (w/v) immediately and put 40min in ambient temperatare, measure the OD value that wavelength is 700nm place.To boil after 30 minutes deactivated zymoprotein under the same terms for blank.
(3) calculate according to calibration curve the Broad Bean Leaves PM H that different I AA concentration sprays
+-ATPase activity (Figure 1B).
4, H
+-pump determination of activity step is as follows:
Use BTP to adjust pH to 6.0 containing 5 mmol/L BTP/MES (pH 6.0), 12 μm of ol/L AO, 300 mmol/L KCl, 250 mmol/L sucrose, 0.5 mmol/L EGTA(in (1) 1.5 ml reaction system), 1 mmol/L NaN
3, 1 mmol/L Na
2moO
4, 50 mmol/L KNO
3, 0.05% Brij-35 (w/v) and 100 μ g plasmalemma proteins; Adding abstergent Brij-35 makes original position film overturn, and after reaction mixture at room temperature places 20 min, adds 5 mmol/L ATP/BTP(pH=6.0) to start reaction;
(2) with reactant liquor zeroing contrast, record per minute OD value, by quencher speed, mensuration acridine orange light absorption value quencher speed in 492 nm place 8 minutes, reflects that the Leaf Cells of Vicia Faba membrane vesicle body of different sodium tungstate spraying concentration pumps H
+ability, i.e. H
+-pump activity (Fig. 2).
As can be seen from Figure 1A, after spraying the sodium tungstate of variable concentrations, the H of Broad Bean Leaves
2o
2content compares with plant in the cabinet not spraying sodium tungstate the minimizing all had in various degree; 1.5 mmol/L sodium tungstate processed group H
2o
2content reduces at most, and concentration of sodium tungstate raises again, H
2o
2content no longer includes significant change.From Figure 1B, after spraying the sodium tungstate of variable concentrations, the PM H of Broad Bean Leaves
+-ATPase activity compares with plant in the cabinet not spraying sodium tungstate the recovery had in various degree, 1.5 mmol/L sodium tungstate processed group H
+-pump activation recovering is the strongest.As can be seen from Figure 2, after spraying the sodium tungstate of variable concentrations, the H of Broad Bean Leaves
+-pump activity compares with plant in the cabinet not spraying sodium tungstate the recovery had in various degree, 1.5 mmol/L sodium tungstate processed group H
+-pump activation recovering is the strongest.H
2o
2, PM H
+-ATPase and H
+-pump is the important regulatory factor in the open activity of plant stomata, H
2o
2content reduces and PM H
+-ATPase, H
+-pump increased activity all contributes to the increase of stomatal aperture or degree of leading, thus promotes stomatal opening.
Embodiment 3: Stoma of Leaves aperture measures.Processed the blade of 1 hour in Example 1 the 3rd step, torn and take off epidermis, be placed on cover glass, dripped a physiological saline, covered, in times Microscopic observation measure stomatal aperture, each concentration random measurement 40, average (Fig. 3).
From Fig. 3, variable concentrations sodium tungstate sprays rear Broad Bean Leaves stomatal aperture recovery in various degree, but 1.5 mmol/L Na
2wO
4maximum to the recovery extent of Broad Bean Leaves stomatal aperture.
Embodiment 4: process 1 hour in embodiment 1 the 3rd step after, start to record residual formaldehyde concentration in gas-tight silo air, 10 minutes records once, record 100 minutes (Fig. 4 A) continuously.And the Leaf Cells of Vicia Faba Free-formaldehyde content having sprayed variable concentrations sodium tungstate in gas-tight silo is measured.Assay method is as follows: get 1.0g Broad Bean Leaves and add the homogenate of 1mL Tris extract liquid nitrogen grinding, proceed to centrifuge tube in 12000g, centrifugal 20min at 4 DEG C, discard residue, supernatant adds 1 mL Nash reagent (acetylacetone,2,4-pentanedione: 0.2%, glacial acetic acid: 0.3%, ammonium acetate: 15%), 30 DEG C of insulations measure OD in 30 minutes afterwards
410, the concentration of blade Free-formaldehyde is calculated according to calibration curve.
From Fig. 3 A, after formaldehyde stress 1h, place sprayed sodium tungstate bean plant gas-tight silo in air formaldehyde decrease speed than not have to place sprayed sodium tungstate bean plant gas-tight silo in soon, the broad bean wherein having sprayed 1.5 mmol/L sodium tungstates makes air formaldehyde in gas-tight silo reduce more, Fig. 3 B demonstrates air formaldehyde in gas-tight silo and reduces relevant with the formaldehyde amount that Broad Bean Leaves absorbs, and the formaldehyde that the Broad Bean Leaves having sprayed 1.5 mmol/L sodium tungstates absorbs is maximum.This illustrates that the ability effect that the sodium tungstate of this concentration improves absorption formaldehyde to Broad Bean Leaves is maximum.
Embodiment 5: according to above-mentioned Leaf Cells of Vicia Faba H
2o
2content, stomatal aperture, cytoplasma membrane H
+the assay result of residual formaldehyde concentration and blade cell Free-formaldehyde in-pump activity, gas-tight silo, filtering out 1.5 mmol/L is the optium concentration that sodium tungstate sprays.In order to verify the facilitation of 1.5 mmol/L sodium tungstates detergent power that gas formaldehyde pollutes in plant is to true indoor environment, Broad Bean Leaves lower epidermis is sprayed with the sodium tungstate treatment fluid of 1.5 mmol/L, the globule is covered with and the amount of spraying of not dripping as often opening blade with lower epidermis, after 8 hours sodium tungstate treatment fluid processing times, broad bean being put into release gas formaldehyde daily mean of concentration is 4mg/m
3timeworn furniture cabinet in, furniture cabinet three is made up of glass door compressed sheet (59 ' 40 ' 48cm), one side, the flowerpot of bean plant is had to put into cabinet rear enclosed cabinet door by long, Stress treatment 48h, each process arranges three repetitions, sprays blade lower epidermis and be placed on the bean plant contrast outside cabinet and in cabinet respectively with water.The conditions such as the illumination in switch board and outside cabinet, temperature and relative moisture are in close level, and illumination and dark processing time are set as 12h/12h.Experimental session round the clock temperature Change and flowerpot encapsulating method the same.Measure that cabinet is outer in 48h, in cabinet in (not spraying sodium tungstate) and cabinet+the plant leaf cell of sodium tungstate (spraying sodium tungstate in cabinet) in H
2o
2content and H
+-pump activity (Fig. 5,6).
Can see from Fig. 5,6, the Broad Bean Leaves having sprayed sodium tungstate in cabinet is compared with the Broad Bean Leaves not spraying sodium tungstate, H
2o
2accumulation obviously declines (Fig. 5 A), PM H
+-ATPase activity (Fig. 5 B) and H
+-pump activity (Fig. 6) obviously raises.This illustrates that the sodium tungstate of 1.5 mmol/L consumes H in Leaf Cells of Vicia Faba
2o
2, and the PM H of Leaf Cells of Vicia Faba film under making formaldehyde stress
+-ATPase and H
+-pump activity is gone up.
Embodiment 6: experimental technique processes with reference to embodiment 5, measures the stomatal aperture, the degree of leading (Fig. 7) that do not spray the Broad Bean Leaves spraying sodium tungstate in sodium tungstate and cabinet in 48h cabinet;
From Fig. 7 A and Fig. 7 B, because gas formaldehyde is coerced, in cabinet, the stomatal aperture of Broad Bean Leaves all obviously declines with degree of leading, but after spraying sodium tungstate, is obtained for obvious recovery by Broad Bean Leaves stomatal aperture in the cabinet of formaldehyde stress and degree of leading.It can thus be appreciated that the sodium tungstate of 1.5 mmol/L contributes to the raising of plant leaf blade stomatal aperture and degree of leading in real formaldehyde pollution air ambient, thus promote plant absorption formaldehyde, purifying formaldehyde pollutes.
Claims (1)
1. sodium tungstate is improving the application that purifies air in formaldehyde pollution of pot plant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410839558.9A CN104541662A (en) | 2014-12-30 | 2014-12-30 | Application of sodium tungstate for improving formaldehyde pollution purification of pot plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410839558.9A CN104541662A (en) | 2014-12-30 | 2014-12-30 | Application of sodium tungstate for improving formaldehyde pollution purification of pot plant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104541662A true CN104541662A (en) | 2015-04-29 |
Family
ID=53059612
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410839558.9A Pending CN104541662A (en) | 2014-12-30 | 2014-12-30 | Application of sodium tungstate for improving formaldehyde pollution purification of pot plant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104541662A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104928316A (en) * | 2015-05-08 | 2015-09-23 | 昆明理工大学 | Plant expression vector of arabidopsis thaliana catalase gene CAT and application of plant expression vector |
| CN107677769A (en) * | 2017-08-10 | 2018-02-09 | 佛山地环科技检测有限公司 | A kind of method and system measured using stifling mode with evaluation plant purification efficiency |
| CN108713471A (en) * | 2018-04-28 | 2018-10-30 | 湖北省烟草科学研究院 | A kind of method of tobacco weather-fleck seedling resistance screening |
| CN109362783A (en) * | 2018-12-12 | 2019-02-22 | 四川国光农化股份有限公司 | A kind of vitaceae is without seed growth regulator composition, preparation and its application |
-
2014
- 2014-12-30 CN CN201410839558.9A patent/CN104541662A/en active Pending
Non-Patent Citations (4)
| Title |
|---|
| 张启翔: "《中国观赏园艺研究进展.2011》", 31 December 2011, 中国林业出版社 * |
| 李艳等: "壳寡糖、一氧化氮和植物激素在烟草气孔运动中的作用及其相互关系", 《植物生理学通讯》 * |
| 蔡宝珍等: "室内植物对甲醛净化性能的研究进展", 《中国农学通报》 * |
| 马庆生: "《生物学大辞典》", 31 January 1999, 广西科学技术出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104928316A (en) * | 2015-05-08 | 2015-09-23 | 昆明理工大学 | Plant expression vector of arabidopsis thaliana catalase gene CAT and application of plant expression vector |
| CN107677769A (en) * | 2017-08-10 | 2018-02-09 | 佛山地环科技检测有限公司 | A kind of method and system measured using stifling mode with evaluation plant purification efficiency |
| CN108713471A (en) * | 2018-04-28 | 2018-10-30 | 湖北省烟草科学研究院 | A kind of method of tobacco weather-fleck seedling resistance screening |
| CN109362783A (en) * | 2018-12-12 | 2019-02-22 | 四川国光农化股份有限公司 | A kind of vitaceae is without seed growth regulator composition, preparation and its application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| El-Tayeb | Response of barley grains to the interactive e. ect of salinity and salicylic acid | |
| Tiwari et al. | Evaluation of ambient air pollution impact on carrot plants at a sub urban site using open top chambers | |
| CN104541662A (en) | Application of sodium tungstate for improving formaldehyde pollution purification of pot plant | |
| Nabaei et al. | Interactive effect of melatonin and sodium nitroprusside on seed germination and seedling growth of Catharanthus roseus under cadmium stress | |
| CN104541659B (en) | A kind of method promoting lobus cardiacus Herba Ardisiae Japonicae seed germination | |
| CN105191984B (en) | A kind of crop protection agents alleviating cadmium infringement and preparation method and application | |
| CN108096086A (en) | A kind of method that the ice plant extract without preservative is prepared using ice plant freeze-dried powder | |
| Selim et al. | Magnetic field treated water effects on germination, growth and physio-chemical aspects of some economic plants | |
| CN108271689A (en) | A kind of method of the indefinite Root tissue culture of wild ginseng | |
| Villiers et al. | The possible role of low temperature in breaking the dormancy of seeds of Fraxinus excelsior L. | |
| Bhoyar et al. | Effects of various dormancy breaking treatments on the germination of wild caper (Capparis spinosa) seeds from the cold arid desert of trans-Himalayas | |
| CN105145023B (en) | A kind of silver drags Chinese cymbidium florescence control method | |
| El-Nashar et al. | Response of Nemesia (Nemesia× hybridus) plants to different irrigation water sources and arbuscular mycorrhizal fungi inoculation | |
| Tang et al. | Role of 5-aminolevulinic acid on growth, photosynthetic parameters and antioxidant enzyme activity in NaCl-stressed Isatis indigotica Fort. | |
| CN104663267A (en) | Method for rapidly and effectively improving drought resistance of tobacco | |
| CN104871847A (en) | Application of ascorbic acid in plant nitrate nitrogen adsorption under increased aluminum stress | |
| Katiyar et al. | Growth behaviour of two cultivars of maize in response to SO~ 2 and NO~ 2 | |
| CN104584897A (en) | New purpose of ascorbic acid | |
| CN104584896A (en) | Application of auxin on aspect of improvement of air formaldehyde pollution purification by potted plant | |
| Brar et al. | Non-thermal plasma treatment of flowing water: A solution to reduce water usage and soil treatment cost without compromising yield | |
| CN104886053B (en) | Application of ascorbic acid to improvement of plant photosynthesis efficiency | |
| CN103909087A (en) | Safe use method of cadmium-polluted vegetable plot | |
| CN202676509U (en) | Device for collecting root exudates of bulbus propagation plants | |
| CN106852209A (en) | A kind of capsicum seeding growing seedlings method | |
| CN106590951A (en) | Big-leaf tea extract detergent and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150429 |
|
| RJ01 | Rejection of invention patent application after publication |