CN104584859A

CN104584859A - Production method for soft edible lucid ganoderma

Info

Publication number: CN104584859A
Application number: CN201510008890.5A
Authority: CN
Inventors: 曾建斌
Original assignee: Individual
Current assignee: Individual
Priority date: 2015-01-01
Filing date: 2015-01-01
Publication date: 2015-05-06

Abstract

With the emergence of soft edible lucid ganoderma, not only is the blank of the human life history filled but also a good eating condition is created for eaters; as woody lucid ganoderma is not convenient for eating, the popularization of the lucid ganoderma is greatly influenced; a new variety is provided for the society successfully by the culture of the edible lucid ganoderma; meanwhile, the emergence of soft edible lucid ganoderma is also an innovative product in the field of science, and a new project for growing rich is provided for society; a great contribution is made for the popularization of the lucid ganoderma, the development of agriculture, farmers and rural areas and health undertaking by the emergence of soft edible lucid ganoderma.

Description

The production method of software edible-type glossy ganoderma

Technical field: the invention belongs to field of edible fungus culture.

Technical background: well-known, glossy ganoderma is a kind of famous and precious traditional Chinese medicine, and it is to chronic bronchitis, coronary heart disease, chronic hepatitis and immunocompromised person, there is good health-care efficacy, because glossy ganoderma is woody cotton product, inconvenience is edible, drastically influence the universal of glossy ganoderma.In order to the life making glossy ganoderma enter people widely, become a kind of daily bread, woody cotton glossy ganoderma must be changed into product edible as mushroom, straw mushroom, be convenient to people and eat, the daily edible product becoming huge numbers of families for glossy ganoderma create good condition.Inventor was from 2008 for this reason, just started the R&D work woody cotton glossy ganoderma being changed into edible-type glossy ganoderma, and through experiment in a few years, edible-type glossy ganoderma is cultivated successfully finally.It is that a kind of sheet grown up on synthetic medium by Ganoderma Lucidum is circular, and the mycelium that meat is pliable and tough, after testing, the composition that it contains is substantially identical with natural glossy ganoderma with health-care efficacy, and without any side effects.The appearance of edible-type glossy ganoderma, has not only filled up a blank in edible mushroom field, and has opened up a new way for the universal of glossy ganoderma.

Ganoderma Lucidum brief introduction: glossy ganoderma Pseudomonas high-temperature strain, energy is saprophytic also can be parasitic, and occurring in nature belongs to Basidiomycotina, Aphyllophorales, glossy ganoderma Cordycepps, Ganoderma.Middle name: polyporus lucidus, latin name: [Ganoderma lucidum (leyss ex fr) karst].

The object of invention: be exactly in order to woody cotton glossy ganoderma is changed into software edible-type kind, make the health products of this preciousness, enter the life of people widely, not only be convenient to the universal of glossy ganoderma, also for society provides a new project of getting rich, for the development of agriculture-countryside-farmer and healthy cause will make major contribution.

The advance of invention: with modern biotechnology, changes into software edible-type kind by woody cotton glossy ganoderma, domesticly yet there are no relevant report, and the birth of edible-type glossy ganoderma belongs to the world initiation, and China is exclusive.

Summary of the invention: get the test tube strains bought from the Chinese Academy of Sciences ready, and then the enough production that spreads cultivation is with bacterium, washes and get blake bottle and required main auxiliary material ready before spreading cultivation, preparation medium, filling, sealing, sterilizing, inoculation, cultivates, gathers, preserves.

Concrete implementation content divides several step: 1, bacterium is used in the test tube strains production that spreads cultivation.2, wash and get blake bottle ready.3, needed raw material is got ready.4, medium is prepared.5, filling, sealing.6, sterilizing.7, cultivate.8, gather, preserve.

Test tube strains spreads cultivation to produce and uses bacterium method: wash the vial getting band aluminium lid ready, in order to unified measurement criteria, adopt bottle height 100mm, external diameter 81mm, wall thickness 1mm, internal diameter of the bottleneck 70mm, bottleneck height 12mm, bottle end diameter 60mm, volume 420ml, blake bottle is with front, first take out after 30 minutes with the potassium permanganate water soaking of 0.1%, one time is rinsed again with clear water, discharge on the table, add 50 grams, disposable rice afterwards, 8 grams of hominy grits, 3 grams of peptones, 5 grams of beef extracts, 3 grams of dusty yeasts, 3 grams of glucose, 5 grams of potato starches, 0.2 gram of potassium dihydrogen phosphate, 0.2 gram of dipotassium hydrogen phosphate, 0.1 gram of magnesium sulfate, 160ml drinking water, 20ml shao-hsing rice wine, finally add 0.01% gram of defoaming powder again, pH value nature, use fire resistant polypropylene film seal afterwards, sterilizing.Sterilising temp 122-124 DEG C, 50 minutes effective sterilizing time, then, when the bottle body temperature after subject to sterilization is down to 23-22 DEG C, inoculation in sterilized space after cleaning, from test tube strains during inoculation, the bacterial classification that picking 1 piece of soya bean is large, is placed on the medium in blake bottle, sealing, and put incubator into immediately, the temperature inside the box controls at 22 DEG C.15 days afterwards unpack examine, as mycelia be covered with cultivate basal plane time, just can be used as production and use bacterium.

Batch production edible-type glossy ganoderma method: spread cultivation and produce with after bacterium, before starting batch production, first gets required blake bottle ready with the potassium permanganate washing containing 0.1%, discharge on the table, for the ease of metering, first add 50 grams of disposable rice in each bottle, 5 grams of corn starchs, 5 grams of potato starches, 3 grams of dried silkworm chrysalis meals, 3 grams of peptones, 3 grams of dusty yeasts, 1 gram of aspartoyl, 0.2 gram of inosine, 5 grams of beef extracts, 3 grams of glucose, 1 gram of sodium chloride, 0.2 gram of potassium dihydrogen phosphate, 0.1 gram of potassium nitrate, 0.2 gram of dipotassium hydrogen phosphate, 0.1 gram of magnesium sulfate, 0.2 gram of Cobastab ₁, 0.2 gram of Cobastab ₁₂, 3 grams of skim milk powder, 160ml drinking water, 20ml shao-hsing rice wine, 0.01% gram of defoaming powder, then fire resistant polypropylene film seal is used, sterilizing, sterilising temp 124-126 DEG C, 50 minutes effective sterilizing time, then afterwards when bottle temperature is down to 20 DEG C, aseptic indoor inoculation again after purification, the bacterial classification that picking 2 pieces of soya beans are large from the bacterium bottle spread cultivation, be placed on the medium in bottle, postvaccinal blake bottle is emitted in culturing room immediately, indoor temperature control is at 25-26 DEG C, humid control is at 60%-65%, indoor not illumination in 20 days, will in dim middle cultivation, within in culturing room one day, ventilate once, be no less than 20 minutes at every turn, when 35 to 40 days, when edible-type Ganoderma lucidum mycelium body thickness reaches more than 5mm, just can gather.Need the adhesive liquor cleaning bottom with clear water after gathering, then exhausted by body surface moisture content with absorbent wool, put into vacuum bag refrigeration immediately, refrigerated storage temperature 8-10 DEG C, does not dry and dries, and is more than whole technique contents of producing edible-type glossy ganoderma.

Claims

1. the production method of edible-type glossy ganoderma is characterized in that following content and step:

Test tube strains spreads cultivation to produce and uses bacterium method: wash the vial getting band aluminium lid ready, in order to unified measurement criteria, adopt bottle height 100mm, external diameter 81mm, wall thickness 1mm, internal diameter of the bottleneck 70mm, bottleneck height 12mm, bottle end diameter 60mm, volume 420ml, blake bottle is with front, first take out after 30 minutes with the potassium permanganate water soaking of 0.1%, one time is rinsed again with clear water, discharge on the table, add 50 grams, disposable rice afterwards, 8 grams of hominy grits, 3 grams of peptones, 5 grams of beef extracts, 3 grams of dusty yeasts, 3 grams of glucose, 5 grams of potato starches, 0.2 gram of potassium dihydrogen phosphate, 0.2 gram of dipotassium hydrogen phosphate, 0.1 gram of magnesium sulfate, 160ml drinking water, 20ml shao-hsing rice wine, finally add 0.01% gram of defoaming powder again, pH value nature, use fire resistant polypropylene film seal afterwards, sterilizing, sterilising temp 122-124 DEG C, 50 minutes effective sterilizing time, then, when the bottle body temperature after subject to sterilization is down to 23-22 DEG C, inoculation in sterilized space after cleaning, from test tube strains during inoculation, the bacterial classification that picking 1 piece of soya bean is large, is placed on the medium in blake bottle, sealing, and put incubator into immediately, the temperature inside the box controls at 22 DEG C, within 15 days, unpack afterwards and examine, as mycelia be covered with cultivate basal plane time, just can be used as and produce with bacterium, spread cultivation and produce with after bacterium, before starting batch production, first get required blake bottle ready with the potassium permanganate washing containing 0.1%, discharge on the table, for the ease of metering, 50 grams of disposable rice are first added in each bottle, 5 grams of corn starchs, 5 grams of potato starches, 3 grams of dried silkworm chrysalis meals, 3 grams of peptones, 3 grams of dusty yeasts, 1 gram of aspartoyl, 0.2 gram of inosine, 5 grams of beef extracts, 3 grams of glucose, 1 gram of sodium chloride, 0.2 gram of potassium dihydrogen phosphate, 0.1 gram of potassium nitrate, 0.2 gram of dipotassium hydrogen phosphate, 0.1 gram of magnesium sulfate, 0.2 gram of vitamin B1, 0.2 gram of cobalamin, 3 grams of skim milk powder, 160ml drinking water, 20ml shao-hsing rice wine, 0.01% gram of defoaming powder, then fire resistant polypropylene film seal is used, sterilizing, sterilising temp 124-126 DEG C, 50 minutes effective sterilizing time, then afterwards when bottle temperature is down to 20 DEG C, aseptic indoor inoculation again after purification, the bacterial classification that picking 2 pieces of soya beans are large from the bacterium bottle spread cultivation, be placed on the medium in bottle, postvaccinal blake bottle is emitted in culturing room immediately, indoor temperature control is at 25-26 DEG C, humid control is at 60%-65%, indoor not illumination in 20 days, will in dim middle cultivation, within in culturing room one day, ventilate once, be no less than 20 minutes at every turn, when 35 to 40 days, when edible-type Ganoderma lucidum mycelium body thickness reaches more than 5mm, just can gather, need the adhesive liquor cleaning bottom with clear water after gathering, then exhausted by body surface moisture content with absorbent wool, put into vacuum bag refrigeration immediately, refrigerated storage temperature 8-10 DEG C, does not dry and dries, and is more than whole technique contents of producing edible-type glossy ganoderma.