CN104561615A - Method for bonding uranium tailing slag by using carbon-nano-material-immobilized mineralization bacterium - Google Patents
Method for bonding uranium tailing slag by using carbon-nano-material-immobilized mineralization bacterium Download PDFInfo
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Abstract
The invention discloses a method for bonding uranium tailing slag by using a carbon-nano-material-immobilized mineralization bacterium. The method is characterized by comprising the following steps: culturing Sporosarcina pasteurii in a liquid culture solution to prepare a high-concentration bacterium solution with the concentration of 1-1.5*10<10>/ml; taking 100g of uranium tailing slag, spraying 5-20ml of 1mg/ml carbon nano material water solution, evenly mixing, and standing for 24 hours; adding 10-30ml of high-concentration bacterium solution, evenly mixing, standing for 2-3 hours, and sucking out the residual bacterium solution; adding 50ml of mineralization culture solution, culturing at 27-33 DEG C for 24 hours, and sucking out the mineralization culture solution to obtain the uranium tailing slag subjected to bonding treatment; repeating the process 5-10 times; and standing the uranium tailing slag after the last time for bonding treatment at 27-33 DEG C for 10-20 days, and bonding the uranium tailing slag into a block with certain permeation resistance and mechanical properties under the mineralization action of the microbe.
Description
Technical field
The invention belongs to the solidification treatment of atomic waste, relate to the method for the cementing uranium mine tailings of the immobilized mineralized bacterium of a kind of carbon nanomaterial.Be specially adapted to using carbon nanomaterial as the cementing uranium mine tailings of mineralized bacterium carrier, thus effectively solve diffusion and the loss of uranium mine tailings.
Background technology
Along with the development of nuclear technique, the demand of people to uranium is increasing, in the recovery process of uranium ore, produces a large amount of uranium mine tailings.China about has 180 many places uranium tailings pond and uranium mine tailings to stack place, and wherein major part is distributed in the ground such as Hunan, Guangzhou, Jiangxi.Owing to being subject to rainwash, pollutent can spread along with rainwater, and can discharge radioactive gas radon in uranium mine tailings, and these all have a strong impact on the safety of environment and people.Uranium tailings pond is a dangerous matter sources with the artificial rubble flow of high potential energy, and the change of geology As time goes on, the integrity of uranium tailings pond may be destroyed, various naturally (rainwater, earthquake, rat hole, earth movement etc.) and artificial (manage improperly, relation is inharmonious) unfavorable factor at every moment or periodically threaten its safety, therefore to the emphasis be processed into study of uranium mine tailings.
In prior art, for the process of uranium mine tailings, usually adopt beach face soverlay technique, namely covered by vegetation, sandy soil or artificial material.Covering material soil, ettle, slag building stones etc., also have scholar once to do the research of artificial material (plastics, pitch etc.); Although the spilling of radioactive gas can be suppressed, working life is not ensured; Although this method can reduce spilling and the rainwash of radioactive gas radon.But As time goes on, often occur aging, problems of crack and lost efficacy, working life does not ensure, and technical process is long, practicality is lower.The Chinese patent application of denomination of invention " a kind of utilize the method for plant adsorption uranium mine tailings ", application number 200910044229.4 discloses a kind of novel method utilizing plant adsorption uranium mine tailings, system adopts uranium enriching plant directly to plant in uranium mine tailings, utilizes the high enriching of plant to uranium to absorb uranium.
Carbon nanomaterial (as carbon fiber, carbon nanotube, graphene oxide) as novel nano-material self the potential superiority that has, have broad application prospects at FIELD OF THE INVENTIONThe such as chemistry, physics, medical science, become the focus that global scientific circles scientific research personnel at different levels falls over each other to pay close attention to.Wherein have spiral, tubular structure carbon nanotube performance outstanding especially, its light weight, there is perfect mechanical property and absorption property, and itself, electromagnetism and chemical property.Because it has good mechanical property and absorption property, there is very large advantage as microorganism carrier.
At present, the treatment process for uranium mine tailings is little, there is not yet the bibliographical information about the cementing uranium mine tailings of the immobilized mineralized bacterium of carbon nanomaterial.
Summary of the invention
Object of the present invention is intended to overcome deficiency of the prior art, provides the method for the cementing uranium mine tailings of the immobilized mineralized bacterium of a kind of carbon nanomaterial, thus effectively solves diffusion and the loss of uranium mine tailings.
Content of the present invention is: the method for the immobilized mineralized bacterium of a kind of carbon nanomaterial cementing (referring to the mineral substance be settled out from pore solution, the effect by loose sediment consolidation gets up) uranium mine tailings, is characterized in that comprising the following steps:
A, prepare high-concentration bacterial liquid: Pasteur's gemma sarcina (Sporosarcina pasteurii) is carried out enlarged culturing in liquid medium, shaking speed per minute 180 ~ 250 turns, cultivate 24 hours at 27 ~ 33 DEG C, bacterium liquid is left the heart 20 ~ 40 minutes with per minute 4000 ~ 5000, abandoning supernatant, collecting precipitation thalline, with deionized water wash 2 ~ 3 times, obtained concentration is every milliliter 1 × 10
10~ 1.5 × 10
10the high-concentration bacterial liquid (i.e. high density Pasteur gemma sarcina bacterium liquid) of individual thalline;
B, uranium mine tailings cementing: by every 100 grams of uranium mine tailings, spray the carbon nanomaterial aqueous solution that 5 ~ 20 ml concns are 1 milligram every milliliter, mix, leave standstill 24 hours (making it fully be adsorbed on uranium mine tailings surface), obtain the uranium mine tailings being adsorbed with carbon nanomaterial; Be adsorbed with in the uranium mine tailings of carbon nanomaterial by 100 grams and add high-concentration bacterial liquid 10 ~ 30 milliliters, mix, leave standstill 2 ~ 3 hours, sucking-off remains bacterium liquid; Add Mineralized Culture liquid 50 milliliters again, at 27 ~ 33 DEG C of temperature, quiescent culture is after 24 hours, and sucking-off Mineralized Culture liquid, obtains the uranium mine tailings after cementing process; Repeat this process 5 ~ 10 times;
C, placement are preserved: placed 10 ~ 20 days at the temperature of 27 ~ 33 DEG C by the uranium mine tailings after last cementing process, obtain the uranium mine tailings that the immobilized mineralized bacterium of carbon nanomaterial is cementing; After above-mentioned process, former loose uranium mine tailings particle is cemented to block by Pasteur's gemma sarcina mineralising, its ultimate compression strength 1 ~ 3MPa, porosity 25 ~ 40%.
In content of the present invention: in the liquid medium described in step a, containing glucose 15 ~ 25 grams, peptone 11 ~ 15 grams, 4 ~ 8 grams, sodium-chlor, potassium primary phosphate 3 ~ 5 grams and dipotassium hydrogen phosphate 3 ~ 5 grams in often liter of liquid medium, the pH value of described liquid medium is 7.0 ~ 8.0.
In content of the present invention: the carbon nanomaterial aqueous solution described in step b is the aqueous solution of carbon fiber, carbon nanotube, graphene oxide etc.
In content of the present invention: in the Mineralized Culture liquid described in step b, containing glucose 15 ~ 25 grams, peptone 11 ~ 15 grams, 4 ~ 8 grams, sodium-chlor, potassium primary phosphate 3 ~ 5 grams, dipotassium hydrogen phosphate 3 ~ 5 grams in often liter of Mineralized Culture liquid, and also containing 3 ~ 5 grams, urea, 5 ~ 7 grams, nitrocalcite in every 100 grams of Mineralized Culture liquid, the pH value of Mineralized Culture liquid is 7.0 ~ 10.0.
In content of the present invention: the particle diameter of uranium mine tailings described in step b is 0.1 ~ 5 millimeter preferably.
Compared with prior art, the present invention has features and beneficial effect:
(1) the present invention is adopted, Pasteur's gemma sarcina can produce in own metabolism activity a large amount of has highly active urease (Urea amidolyase), urease activity is found in large-scale microorganism and plant, Pasteur's gemma sarcina can take urea as the energy, produce ammonia and carbonic acid gas by urease hydrolyze urea, chemical equation is as follows:
Ammonia makes environment pH improve, and impels the calcium ion of external source to be combined with carbonate, and form calcium carbonate crystal, and mainly deposit with the form of calcite, this process reaction is as follows:
In the process, Pasteur's gemma sarcina seldom produces toxicant or other by products, and the cell of Pasteur's gemma sarcina can not be assembled, and this ensures that it has the long-pending and volume ratio of a high cell surface.These features all make Pasteur's gemma sarcina possess ability for practical application above; Pasteur's gemma sarcina provide not only the formation site of calcium carbonate nucleus in the process of its induction tosca, but also a generation alkaline environment induces calcium carbonate to deposit further.
(2) principle of the inventive method is: after carbon nanomaterial is adsorbed on uranium mine tailings surface, thalline is using carbon nanomaterial as carrier; Because carbon nanomaterial has good absorption property, energy useful load microorganism, finally makes thalline and uranium mine tailings be uniformly distributed; In inoculum, urea is broken down into carbonate under the effect of urase; Calcium ion attracts bacteria phage surface in solution, and be combined with carbonate and form calcium carbonate; The calcium carbonate crystal that microorganism induction is formed serves as function served as bridge between discrete particle, thus discrete particle consolidation is become the entirety with certain mechanical property; Thus effectively solve diffusion and the loss of uranium mine tailings;
(3) adopt the present invention, inject carbon nanomaterial in uranium mine tailings as microorganism carrier, make it fully be adsorbed on mine tailings surface; Reinject concentrated bacterium liquid, and static rear sucking-off remains bacterium liquid; Mineralized Culture of reinjecting liquid, after cultivation, sucking-off Mineralized Culture liquid; Repeat this process repeatedly; Final microorganism take urea as substrate, uranium mine tailings is consolidated into the entirety with certain permeability resistance and mechanical property by the mineralization of microorganism; Because the carbon nanomaterial wherein adulterated has good absorption property and mechanical property, make cementing after overall mechanical property improve further;
(4) the inventive method cost low, simple to operate, can not secondary pollution be caused, have broad application prospects, practical.
Embodiment
The invention will be further described for embodiment plan given below; but can not be interpreted as it is limiting the scope of the invention; some nonessential improvement and adjustment that person skilled in art makes the present invention according to the content of the invention described above, still belong to protection scope of the present invention.
Embodiment 1:
Get uranium mine tailings 100 grams, in uranium mine tailings, add the carbon fiber water solution 5 milliliters that concentration is 1 milligram every milliliter, mix, leave standstill 24 hours.Put one deck filter paper in die trial (can be upper end open, the porose cylindrical mold in bottom) bottom, uranium mine tailings is put into die trial, uranium mine tailings puts one deck filter paper again, play shock absorption.Pasteur's gemma sarcina is carried out enlarged culturing in liquid medium, and often liter of liquid medium composition and content are: glucose 20 grams, peptone 12 grams, 5 grams, sodium-chlor, potassium primary phosphate 3 grams, dipotassium hydrogen phosphate 3 grams; Liquid medium pH value is 8.0.Cultivate 24 hours at 30 DEG C, bacterium liquid is left the heart 20 minutes with 4000, obtains concentrated bacterium liquid.With the flow velocity of per minute 5 milliliters, 15 milliliters of concentrated bacterium liquid are injected uranium mine tailings with peristaltic pump, stagnate after 2.5 hours and release bacterium liquid (namely sucking-off remains bacterium liquid), 50 milliliters of Mineralized Culture liquid are injected with the flow velocity of per minute 10 milliliters again with peristaltic pump, often liter of Mineralized Culture liquid composition and content are: glucose 20 grams, peptone 11 grams, 5 grams, sodium-chlor, potassium primary phosphate 3 grams, dipotassium hydrogen phosphate 3 grams; And also containing 3 grams, urea, 5 grams, nitrocalcite in every 100 grams of Mineralized Culture liquid, the pH value of Mineralized Culture liquid is 8.0.Constant temperature 30 DEG C is cultivated after 24 hours, and Mineralized Culture liquid is released (i.e. sucking-off Mineralized Culture liquid).Repeat this process 5 times.Constant temperature is placed, preserve: placed 10 days by the uranium mine tailings constant temperature 30 DEG C after cementing for the last time, take out and preserve.
Test result is: ultimate compression strength: 1.5MPa, porosity: 35.3%.
Embodiment 2:
Get uranium mine tailings 100 grams, in uranium mine tailings, add the carbon fiber water solution 10 milliliters that concentration is 1 milligram every milliliter, mix, leave standstill 24 hours.Put one deck filter paper in die trial (can be upper end open, the porose cylindrical mold in bottom) bottom, uranium mine tailings is put into die trial, uranium mine tailings puts one deck filter paper again, play shock absorption.Pasteur's gemma sarcina is carried out enlarged culturing in liquid medium, and often liter of liquid medium composition and content are: glucose 20 grams, peptone 11 grams, 5 grams, sodium-chlor, potassium primary phosphate 3 grams, dipotassium hydrogen phosphate 3 grams; Liquid medium pH value is 8.0.Cultivate 24 hours at 30 DEG C, bacterium liquid is left the heart 20 minutes with 4000, obtains concentrated bacterium liquid.With the flow velocity of per minute 5 milliliters, 15 milliliters of concentrated bacterium liquid are injected uranium mine tailings with peristaltic pump, stagnate after 2.5 hours and release bacterium liquid (namely sucking-off remains bacterium liquid), 50 milliliters of Mineralized Culture liquid are injected with the flow velocity of per minute 8 milliliters again with peristaltic pump, often liter of Mineralized Culture liquid composition and content are: glucose 20 grams, peptone 11 grams, 5 grams, sodium-chlor, potassium primary phosphate 3 grams, dipotassium hydrogen phosphate 3 grams; And also containing 3 grams, urea, 5 grams, nitrocalcite in every 100 grams of Mineralized Culture liquid, the pH value of Mineralized Culture liquid is 8.0.Constant temperature 30 DEG C is cultivated after 24 hours, and Mineralized Culture liquid is released (i.e. sucking-off Mineralized Culture liquid).Repeat this process 5 times.Constant temperature is placed, preserve: placed 12 days by the uranium mine tailings constant temperature 30 DEG C after cementing for the last time, take out and preserve.
Test result is: ultimate compression strength: 2.0MPa, porosity: 35.6%.
Embodiment 3:
Get uranium mine tailings 100 grams, in uranium mine tailings, add the carbon fiber water solution 8 milliliters that concentration is 1 milligram every milliliter, mix, leave standstill 24 hours.Put one deck filter paper in die trial (can be upper end open, the porose cylindrical mold in bottom) bottom, uranium mine tailings is put into die trial, uranium mine tailings puts one deck filter paper again, play shock absorption.Pasteur's gemma sarcina is carried out enlarged culturing in liquid medium, and often liter of liquid medium composition and content are: glucose 20 grams, peptone 11 grams, 5 grams, sodium-chlor, potassium primary phosphate 4 grams, dipotassium hydrogen phosphate 4 grams; Liquid medium pH value is 7.0.Cultivate 24 hours at 30 DEG C, bacterium liquid is left the heart 30 minutes with 4500, obtains concentrated bacterium liquid.With the flow velocity of 7mL/min, 20 milliliters of concentrated bacterium liquid are injected uranium mine tailings with peristaltic pump, stagnate after 2.5 hours and release bacterium liquid (namely sucking-off remains bacterium liquid), 50 milliliters of Mineralized Culture liquid are injected with the flow velocity of per minute 9 milliliters again with peristaltic pump, often liter of Mineralized Culture liquid composition and content are: glucose 20 grams, peptone 11 grams, 5 grams, sodium-chlor, potassium primary phosphate 4 grams, dipotassium hydrogen phosphate 4 grams; And also containing 4 grams, urea, 7 grams, nitrocalcite in every 100 grams of Mineralized Culture liquid, the pH value of Mineralized Culture liquid is 7.0.Constant temperature 32 DEG C is cultivated after 24 hours, and Mineralized Culture liquid is released (i.e. sucking-off Mineralized Culture liquid).Repeat this process 5 times.Constant temperature is placed, preserve: placed 15 days by the uranium mine tailings constant temperature 30 DEG C after cementing for the last time, take out and preserve.Test result is: ultimate compression strength: 2.7MPa, porosity: 32%.
Embodiment 4:
Get uranium mine tailings 100 grams, in uranium mine tailings, add the carbon fiber water solution 10 milliliters that concentration is 1 milligram every milliliter, mix, leave standstill 24 hours.Put one deck filter paper in die trial (can be upper end open, the porose cylindrical mold in bottom) bottom, uranium mine tailings is put into die trial, uranium mine tailings puts one deck filter paper again, play shock absorption.Pasteur's gemma sarcina is carried out enlarged culturing in liquid medium, and often liter of liquid medium composition and content are: glucose 20 grams, peptone 11 grams, 5 grams, sodium-chlor, potassium primary phosphate 4 grams, dipotassium hydrogen phosphate 4 grams; Liquid medium pH value is 7.0.Cultivate 24 hours at 30 DEG C, bacterium liquid is left the heart 40 minutes with 4500, obtains concentrated bacterium liquid.With the flow velocity of per minute 8 milliliters, 20 milliliters of concentrated bacterium liquid are injected uranium mine tailings with peristaltic pump, stagnate after 3 hours and release bacterium liquid (namely sucking-off remains bacterium liquid), 50 milliliters of Mineralized Culture liquid are injected with the flow velocity of per minute 5 milliliters again with peristaltic pump, often liter of Mineralized Culture liquid composition and content are: glucose 20 grams, peptone 11 grams, 5 grams, sodium-chlor, potassium primary phosphate 4 grams, dipotassium hydrogen phosphate 4 grams; And also containing 4 grams, urea, 7 grams, nitrocalcite in every 100 grams of Mineralized Culture liquid, the pH value of Mineralized Culture liquid is 7.0.Constant temperature 32 DEG C is cultivated after 24 hours, and Mineralized Culture liquid is released (i.e. sucking-off Mineralized Culture liquid).Repeat this process 5 times.Constant temperature is placed, preserve: placed 17 days by the uranium mine tailings constant temperature 30 DEG C after cementing for the last time, take out and preserve.Test result is: ultimate compression strength: 1.8MPa, porosity: 34%.
Embodiment 5:
Get uranium mine tailings 100 grams, in uranium mine tailings, add the carbon fiber water solution 10 milliliters that concentration is 1 milligram every milliliter, mix, leave standstill 24 hours.Put one deck filter paper in die trial (can be upper end open, the porose cylindrical mold in bottom) bottom, uranium mine tailings is put into die trial, uranium mine tailings puts one deck filter paper again, play shock absorption.Pasteur's gemma sarcina is carried out enlarged culturing in liquid medium, and often liter of liquid medium composition and content are: glucose 22 grams, peptone 15 grams, 5 grams, sodium-chlor, potassium primary phosphate 3.5 grams, dipotassium hydrogen phosphate 3.5 grams; Liquid medium pH value is 8.0.Cultivate 24 hours at 30 DEG C, bacterium liquid is left the heart 25 minutes with 5000, obtains concentrated bacterium liquid.With the flow velocity of per minute 10 milliliters, 25 milliliters of concentrated bacterium liquid are injected uranium mine tailings with peristaltic pump, stagnate after 3 hours and release bacterium liquid (namely sucking-off remains bacterium liquid), 50 milliliters of Mineralized Culture liquid are injected with the flow velocity of per minute 10 milliliters again with peristaltic pump, often liter of Mineralized Culture liquid composition and content are: glucose 22 grams, peptone 11 grams, 5 grams, sodium-chlor, potassium primary phosphate 3.5 grams, dipotassium hydrogen phosphate 3.5 grams; And also containing 5 grams, urea, 7 grams, nitrocalcite in every 100 grams of Mineralized Culture liquid, the pH value of Mineralized Culture liquid is 8.0.Constant temperature 32 DEG C is cultivated after 24 hours, and Mineralized Culture liquid is released (i.e. sucking-off Mineralized Culture liquid).Repeat this process 5 times.Constant temperature is placed, preserve: placed 20 days by the uranium mine tailings constant temperature 30 DEG C after cementing for the last time, take out and preserve.Test result is: ultimate compression strength: 2.8MPa, porosity: 33.5%.
Embodiment 6:
A method for the cementing uranium mine tailings of the immobilized mineralized bacterium of carbon nanomaterial, comprises the following steps:
A, prepare high-concentration bacterial liquid: Pasteur's gemma sarcina (Sporosarcina pasteurii) is carried out enlarged culturing in liquid medium, shaking speed per minute 180 turns, cultivate 24 hours at 27 DEG C, bacterium liquid is left the heart 20 minutes with per minute 4000, abandoning supernatant, collecting precipitation thalline, with deionized water wash 2 times, obtained concentration is every milliliter 1 × 10
10~ 1.5 × 10
10high density (Pasteur's gemma sarcina) bacterium liquid in individual thalline scope;
In described liquid medium, containing glucose 15 grams, peptone 11 grams, 4 grams, sodium-chlor, potassium primary phosphate 3 grams and dipotassium hydrogen phosphate 3 grams in often liter of liquid medium, the pH value of described liquid medium is in 7.0 ~ 8.0 scopes;
B, uranium mine tailings cementing: by every 100 grams of uranium mine tailings, spray the carbon nanomaterial aqueous solution that 5 ml concns are 1 milligram every milliliter, mix, leave standstill 24 hours (making it fully be adsorbed on uranium mine tailings surface), obtain the uranium mine tailings being adsorbed with carbon nanomaterial; Be adsorbed with in the uranium mine tailings of carbon nanomaterial by 100 grams and add high-concentration bacterial liquid 10 milliliters, mix, leave standstill 2 hours, sucking-off remains bacterium liquid; Add Mineralized Culture liquid 50 milliliters again, at 27 DEG C of temperature, quiescent culture is after 24 hours, and sucking-off Mineralized Culture liquid, obtains the uranium mine tailings after cementing process; Repeat this process 5 times;
In described Mineralized Culture liquid, containing glucose 15 grams, peptone 11 grams, 4 grams, sodium-chlor, potassium primary phosphate 3 grams, dipotassium hydrogen phosphate 3 grams in often liter of Mineralized Culture liquid, and also containing 3 grams, urea, 5 grams, nitrocalcite in every 100 grams of Mineralized Culture liquid, the pH value of Mineralized Culture liquid is in 7.0 ~ 10.0 scopes;
C, placement are preserved: placed 10 days at the temperature of 27 ~ 33 DEG C by the uranium mine tailings after last cementing process, obtain the uranium mine tailings that the immobilized mineralized bacterium of carbon nanomaterial is cementing.
Embodiment 7:
A method for the cementing uranium mine tailings of the immobilized mineralized bacterium of carbon nanomaterial, comprises the following steps:
A, prepare high-concentration bacterial liquid: Pasteur's gemma sarcina (Sporosarcina pasteurii) is carried out enlarged culturing in liquid medium, shaking speed per minute 250 turns, cultivate 24 hours at 33 DEG C, bacterium liquid is left the heart 40 minutes with per minute 5000, abandoning supernatant, collecting precipitation thalline, with deionized water wash 3 times, obtained concentration is every milliliter 1 × 10
10~ 1.5 × 10
10high density (Pasteur's gemma sarcina) bacterium liquid in individual thalline scope;
In described liquid medium, containing glucose 25 grams, peptone 15 grams, 8 grams, sodium-chlor, potassium primary phosphate 5 grams and dipotassium hydrogen phosphate 5 grams in often liter of liquid medium, the pH value of described liquid medium is in 7.0 ~ 8.0 scopes;
B, uranium mine tailings cementing: by every 100 grams of uranium mine tailings, spray the carbon nanomaterial aqueous solution that 20 ml concns are 1 milligram every milliliter, mix, leave standstill 24 hours (making it fully be adsorbed on uranium mine tailings surface), obtain the uranium mine tailings being adsorbed with carbon nanomaterial; Be adsorbed with in the uranium mine tailings of carbon nanomaterial by 100 grams and add high-concentration bacterial liquid 30 milliliters, mix, leave standstill 3 hours, sucking-off remains bacterium liquid; Add Mineralized Culture liquid 50 milliliters again, at 33 DEG C of temperature, quiescent culture is after 24 hours, and sucking-off Mineralized Culture liquid, obtains the uranium mine tailings after cementing process; Repeat this process 10 times;
In described Mineralized Culture liquid, containing glucose 25 grams, peptone 15 grams, 8 grams, sodium-chlor, potassium primary phosphate 5 grams, dipotassium hydrogen phosphate 5 grams in often liter of Mineralized Culture liquid, and also containing 5 grams, urea, 7 grams, nitrocalcite in every 100 grams of Mineralized Culture liquid, the pH value of Mineralized Culture liquid is in 7.0 ~ 10.0 scopes;
C, placement are preserved: placed 20 days at the temperature of 33 DEG C by the uranium mine tailings after last cementing process, obtain the uranium mine tailings that the immobilized mineralized bacterium of carbon nanomaterial is cementing.
Embodiment 8:
A method for the cementing uranium mine tailings of the immobilized mineralized bacterium of carbon nanomaterial, comprises the following steps:
A, prepare high-concentration bacterial liquid: Pasteur's gemma sarcina (Sporosarcina pasteurii) is carried out enlarged culturing in liquid medium, shaking speed per minute 220 turns, cultivate 24 hours at 30 DEG C, bacterium liquid is left the heart 30 minutes with per minute 4500, abandoning supernatant, collecting precipitation thalline, with deionized water wash 3 times, obtained concentration is every milliliter 1 × 10
10~ 1.5 × 10
10high density (Pasteur's gemma sarcina) bacterium liquid in individual thalline scope;
In described liquid medium, containing glucose 20 grams, peptone 13 grams, 6 grams, sodium-chlor, potassium primary phosphate 4 grams and dipotassium hydrogen phosphate 4 grams in often liter of liquid medium, the pH value of described liquid medium is in 7.0 ~ 8.0 scopes;
B, uranium mine tailings cementing: by every 100 grams of uranium mine tailings, spray the carbon nanomaterial aqueous solution that 12 ml concns are 1 milligram every milliliter, mix, leave standstill 24 hours (making it fully be adsorbed on uranium mine tailings surface), obtain the uranium mine tailings being adsorbed with carbon nanomaterial; Be adsorbed with in the uranium mine tailings of carbon nanomaterial by 100 grams and add high-concentration bacterial liquid 20 milliliters, mix, leave standstill 2.5 hours, sucking-off remains bacterium liquid; Add Mineralized Culture liquid 50 milliliters again, at 30 DEG C of temperature, quiescent culture is after 24 hours, and sucking-off Mineralized Culture liquid, obtains the uranium mine tailings after cementing process; Repeat this process 8 times;
In described Mineralized Culture liquid, containing glucose 20 grams, peptone 13 grams, 6 grams, sodium-chlor, potassium primary phosphate 4 grams, dipotassium hydrogen phosphate 4 grams in often liter of Mineralized Culture liquid, and also containing 4 grams, urea, 6 grams, nitrocalcite in every 100 grams of Mineralized Culture liquid, the pH value of Mineralized Culture liquid is in 7.0 ~ 10.0 scopes;
C, placement are preserved: placed 15 days at the temperature of 30 DEG C by the uranium mine tailings after last cementing process, obtain the uranium mine tailings that the immobilized mineralized bacterium of carbon nanomaterial is cementing.
Embodiment 9 ~ 15:
A method for the cementing uranium mine tailings of the immobilized mineralized bacterium of carbon nanomaterial, comprises the following steps:
A, prepare high-concentration bacterial liquid: Pasteur's gemma sarcina (Sporosarcina pasteurii) is carried out enlarged culturing in liquid medium, shaking speed per minute 180 ~ 250 turns, cultivate 24 hours at 27 ~ 33 DEG C, bacterium liquid is left the heart 20 ~ 40 minutes with per minute 4000 ~ 5000, abandoning supernatant, collecting precipitation thalline, with deionized water wash 2 ~ 3 times, obtained concentration is every milliliter 1 × 10
10~ 1.5 × 10
10high density (Pasteur's gemma sarcina) bacterium liquid in individual thalline scope;
In described liquid medium, containing glucose 15 ~ 25 grams, peptone 11 ~ 15 grams, 4 ~ 8 grams, sodium-chlor, potassium primary phosphate 3 ~ 5 grams and dipotassium hydrogen phosphate 3 ~ 5 grams in often liter of liquid medium, the pH value of described liquid medium is in 7.0 ~ 8.0 scopes;
See the following form containing each feed composition quality consumption (gram) in often liter of liquid medium in each embodiment:
B, uranium mine tailings cementing: by every 100 grams of uranium mine tailings, spray the carbon nanomaterial aqueous solution that 5 ~ 20 ml concns are 1 milligram every milliliter, mix, leave standstill 24 hours (making it fully be adsorbed on uranium mine tailings surface), obtain the uranium mine tailings being adsorbed with carbon nanomaterial; Be adsorbed with in the uranium mine tailings of carbon nanomaterial by 100 grams and add high-concentration bacterial liquid 10 ~ 30 milliliters (in embodiment 9 ~ 15, consumption can be respectively: 12,16,19,22,25,27,29 milliliters), mix, leave standstill 2 ~ 3 hours, sucking-off remains bacterium liquid; Add Mineralized Culture liquid 50 milliliters again, at 27 ~ 33 DEG C of temperature, quiescent culture is after 24 hours, and sucking-off Mineralized Culture liquid, obtains the uranium mine tailings after cementing process; Repeat this process 5 ~ 10 times;
In described Mineralized Culture liquid, containing glucose 15 ~ 25 grams, peptone 11 ~ 15 grams, 4 ~ 8 grams, sodium-chlor, potassium primary phosphate 3 ~ 5 grams, dipotassium hydrogen phosphate 3 ~ 5 grams in often liter of Mineralized Culture liquid, and also containing 3 ~ 5 grams, urea, 5 ~ 7 grams, nitrocalcite in every 100 grams of Mineralized Culture liquid, the pH value of Mineralized Culture liquid is in 7.0 ~ 10.0 scopes;
See the following form containing each feed composition quality consumption (gram) in often liter of liquid medium in each embodiment:
C, placement are preserved: placed at the temperature of 27 ~ 33 DEG C by the uranium mine tailings after last cementing process 10 ~ 20 days (can be respectively in embodiment 9 ~ 15: 10,12,14,16,17,18,19 days), obtain the uranium mine tailings that the immobilized mineralized bacterium of carbon nanomaterial is cementing.
In above-described embodiment 6 ~ 15: after each step process of method of the cementing uranium mine tailings of the immobilized mineralized bacterium of described carbon nanomaterial, former loose uranium mine tailings particle is cemented to block by Pasteur's gemma sarcina mineralising, its ultimate compression strength 1 ~ 3MPa, porosity 25 ~ 40%.
In above-described embodiment 6 ~ 15: the carbon nanomaterial aqueous solution described in step b can be the aqueous solution of carbon fiber, carbon nanotube, graphene oxide etc.
In above-described embodiment 6 ~ 15: the particle diameter of uranium mine tailings described in step b is 0.1 ~ 5 millimeter preferably.
In above-described embodiment: each raw material adopted is commercially available prod.
In above-described embodiment: in the percentage adopted, do not indicate especially, be quality (weight) percentage or well known to a person skilled in the art percentage; Described quality (weight) part can be all gram or kilogram.
In above-described embodiment: the processing parameter (temperature, time, concentration etc.) in each step and each amounts of components numerical value etc. are scope, and any point is all applicable.
The concrete same prior art of technology contents described in content of the present invention and above-described embodiment.
The invention is not restricted to above-described embodiment, all can implement described in content of the present invention and there is described good result.
Claims (5)
1. a method for the cementing uranium mine tailings of the immobilized mineralized bacterium of carbon nanomaterial, is characterized in that comprising the following steps:
A, prepare high-concentration bacterial liquid: Pasteur's gemma sarcina is carried out enlarged culturing in liquid medium, shaking speed per minute 180 ~ 250 turns, cultivate 24 hours at 27 ~ 33 DEG C, bacterium liquid is left the heart 20 ~ 40 minutes with per minute 4000 ~ 5000, abandoning supernatant, collecting precipitation thalline, with deionized water wash 2 ~ 3 times, obtained concentration is every milliliter 1 × 10
10~ 1.5 × 10
10the high-concentration bacterial liquid of individual thalline;
B, uranium mine tailings cementing: by every 100 grams of uranium mine tailings, sprays the carbon nanomaterial aqueous solution that 5 ~ 20 ml concns are 1 milligram every milliliter, mixes, leave standstill 24 hours, obtain the uranium mine tailings being adsorbed with carbon nanomaterial; Be adsorbed with in the uranium mine tailings of carbon nanomaterial by 100 grams and add high-concentration bacterial liquid 10 ~ 30 milliliters, mix, leave standstill 2 ~ 3 hours, sucking-off remains bacterium liquid; Add Mineralized Culture liquid 50 milliliters again, at 27 ~ 33 DEG C of temperature, quiescent culture is after 24 hours, and sucking-off Mineralized Culture liquid, obtains the uranium mine tailings after cementing process; Repeat this process 5 ~ 10 times;
C, placement are preserved: placed 10 ~ 20 days at the temperature of 27 ~ 33 DEG C by the uranium mine tailings after last cementing process, obtain the uranium mine tailings that the immobilized mineralized bacterium of carbon nanomaterial is cementing.
2. by the method for the cementing uranium mine tailings of the immobilized mineralized bacterium of carbon nanomaterial described in claim 1, it is characterized in that: in the liquid medium described in step a, containing glucose 15 ~ 25 grams, peptone 11 ~ 15 grams, 4 ~ 8 grams, sodium-chlor, potassium primary phosphate 3 ~ 5 grams and dipotassium hydrogen phosphate 3 ~ 5 grams in often liter of liquid medium, the pH value of described liquid medium is 7.0 ~ 8.0.
3., by the method for the cementing uranium mine tailings of the immobilized mineralized bacterium of carbon nanomaterial described in claim 1, it is characterized in that: the carbon nanomaterial aqueous solution described in step b is the aqueous solution of carbon fiber, carbon nanotube, graphene oxide.
4. by the method for the cementing uranium mine tailings of the immobilized mineralized bacterium of carbon nanomaterial described in claim 1, it is characterized in that: in the Mineralized Culture liquid described in step b, containing glucose 15 ~ 25 grams, peptone 11 ~ 15 grams, 4 ~ 8 grams, sodium-chlor, potassium primary phosphate 3 ~ 5 grams, dipotassium hydrogen phosphate 3 ~ 5 grams in often liter of Mineralized Culture liquid, and also containing 3 ~ 5 grams, urea, 5 ~ 7 grams, nitrocalcite in every 100 grams of Mineralized Culture liquid, the pH value of Mineralized Culture liquid is 7.0 ~ 10.0.
5., by the method for the cementing uranium mine tailings of the immobilized mineralized bacterium of carbon nanomaterial described in claim 1, it is characterized in that: the particle diameter of uranium mine tailings described in step b is 0.1 ~ 5 millimeter.
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