CN104558256A - Method for pulsed electric field coupled resistance heating-enhanced assisting countercurrent extraction of inulin - Google Patents
Method for pulsed electric field coupled resistance heating-enhanced assisting countercurrent extraction of inulin Download PDFInfo
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- CN104558256A CN104558256A CN201510006439.XA CN201510006439A CN104558256A CN 104558256 A CN104558256 A CN 104558256A CN 201510006439 A CN201510006439 A CN 201510006439A CN 104558256 A CN104558256 A CN 104558256A
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Abstract
The invention discloses a method for pulsed electric field coupled resistance heating-enhanced assisting countercurrent extraction of inulin. The method comprises the following specific steps: cleaning jerusalem artichoke roots and cutting into jerusalem artichoke root shreds having uniform sizes with a rotary automatic shredding machine, placing the jerusalem artichoke root shreds in a treatment chamber between two electrodes of which the distance is 5-10 cm in the pulsed electric field, adding an inulin extractive liquid until the jerusalem artichoke root shreds are immersed, carrying out pulsed electric field coupled resistance heating pretreatment by adjusting the pulse period intervals and placing the jerusalem artichoke root shreds in a countercurrent extractor for extracting inulin. According to the method, by coupling the electroporation phenomenon of the pulsed electric field and the thermal effects of resistance heating, under the synergistic effect of the pulsed electric field and resistance heating, the plant cell structures are effectively loosened by virtue of short-time high temperature, the expansion of the electroporation effect of the pulsed electric field is facilitated, the mass transfer of plant cells is promoted and the diffusion effect of inulin from the cells to the extractive liquid is enhanced. Compared with the traditional extraction process, the technology disclosed by the invention has the advantages of high speed, energy conservation, low-temperature extraction and the like and the extraction rate of inulin is increased.
Description
Technical field
The present invention relates to food engineering and functional ingredient preparing technical field, specifically the method for the auxiliary countercurrent extraction inulin of a kind of pulsed electrical field repeating resistance heating strengthening.
Background technology
Inulin is a kind of Polylevulosan, is formed by fructose molecule aggregation.It is a kind of water-soluble dietary fibre, and due to the molecular structure of its uniqueness, inulin has reducing blood-fat, improves intestinal environment, promotes proliferation of probiotics, promotes the physiological functions such as mineral absorption.Inulin does not cause blood glucose fluctuation after eating in addition, can be diabetes patient.Be widely used as foodstuff additive, with strengthening immunity, assist fat-reducing, promote tooth and gums healthy.Inulin is present in multiple natural phant, wherein the highest with content in Garden Dahlia root, jerusalem artichoke/Jerusalem artichoke root and root of Herba Cichorii.According to plantation climate requirements, Europe is main is that inulin produced by starting material with root of Herba Cichorii, and China mainly with jerusalem artichoke root for inulin produced by starting material.
Tradition inulin industrial extraction method is mainly solvent with water, high temperature extraction.Root of Herba Cichorii is placed in 80-90 after chopping
obe that extracting solution extracts with water in C countercurrent extractor, extraction time is 1.5-2h, water/witloof rhizoid ratio is 1.4/1, after obtaining inulin extracting solution, add liming by carbonating and pass into carbonic acid gas adjust ph to produce precipitation to reach the pectin removed in extracting solution, the object of the impurity such as protein.The inulin solution of preliminary purification through ion exchange resin demineralization, then through activated carbon decolorizing, eventually pass evaporation and spraying dry carry out the inulin powder of obtained purity more than 93%.
In order to ensure higher inulin extraction yield, higher Extracting temperature and longer extraction time is have employed in current technology, Extracting temperature is 80-90 DEG C, extraction time is 1.5-2h, but the utilization of high temperature had both added the consumption of energy, also destroy the cell walls of root of Herba Cichorii cell simultaneously, thus cause more thermo-sensitivity impurity to enter extracting solution, affect extracting solution purity.In recent years, pulsed electrical field strengthening extracting method is widely used in the extraction of effective ingredients in plant.The electroporation phenomenon utilizing pulsed electrical field to produce, the cytolemma of vegetable cell can be made to produce reversible or irreversible aperture, and cell walls keeps its integrity, thus reaches the object of extract at low temperature and selective extraction.
Chinese patent CN201210172455.2 discloses a kind of pulsed electric field that adopts and produces Ganoderma extract method fast.Chinese patent CN201310141353.9 discloses the device and method that a kind of pulsed electrical field assists countercurrent extraction grape skin polyphenol.Resistive heating is also a kind of method strengthening material transfer in leaching process on the other hand.But single extraction strengthening means have its limitation, pulsed electrical field extraction efficiency turnout improves limited, and long-time resistive heating easily produces superheating phenomenon, and inulin macromolecular chain is degraded, and is unfavorable for producing the uniform inulin product of molecular chain.
Summary of the invention
The object of the present invention is to provide the method for the auxiliary countercurrent extraction inulin of pulsed electrical field repeating resistance heating strengthening that a kind of extraction efficiency is high, Extracting temperature is low, to solve the problem proposed in above-mentioned background technology.
For achieving the above object, the invention provides following technical scheme:
A method for the auxiliary countercurrent extraction inulin of pulsed electrical field repeating resistance heating strengthening, concrete steps are as follows:
(1) choose the jerusalem artichoke root of fresh mature, after being cleaned by jerusalem artichoke root, be cut into uniform jerusalem artichoke rhizoid with rotary automatic shredding machine;
(2) above-mentioned jerusalem artichoke rhizoid is placed in pulsed electrical field apart from the treatment chamber between two electrodes for 5-10cm, add inulin extracting solution to not having all jerusalem artichoke rhizoids, pulsed electrical field repeating resistance heat pre-treatment is carried out by adjustment recurrence interval interval, pulse strength is 500-1000v/cm, pulse duration 50-100 μ s, the recurrent interval is 100-200 μ s, has 30-100 umber of pulse in each recurrence interval, recurrence interval number 5-100, the recurrence interval is spaced apart 1-5s;
(3) above-mentioned jerusalem artichoke rhizoid is placed in countercurrent extractor and carries out inulin extraction, Extracting temperature is 30-80 DEG C, extraction time 60-120min.
As the further scheme of the present invention: in described inulin extracting solution, inulin is 10-20% according to the content of the content amount percentage ratio of mass percent.
As the further scheme of the present invention: the extraction agent in described countercurrent extractor is water, the mass ratio of extraction agent and jerusalem artichoke rhizoid is 1.2-2.5:1.
As the further scheme of the present invention: described electrode adopts plate electrode.
As the present invention's further scheme: the material of described electrode is iron, copper or aluminium.
Compared with prior art, the invention has the beneficial effects as follows:
The present invention has been coupled the electroporation phenomenon of pulsed electrical field and the heat effect of resistive heating, under the synergy of pulsed electrical field and resistive heating, utilize the effectively loose plant cell structures of the high temperature of short period of time, the expansion being more conducive to pulsed electric field electroporation effect realizes, promote the mass transfer of vegetable cell, strengthen inulin from cell to the diffusion extracting solution, reduce the temperature needed for extraction, improve extraction efficiency.
Accompanying drawing explanation
Fig. 1 is schematic flow sheet of the present invention.
Embodiment
Be described in more detail below in conjunction with the technical scheme of embodiment to this patent.
Embodiment 1
Refer to Fig. 1, in the embodiment of the present invention, the method for the auxiliary countercurrent extraction inulin of a kind of pulsed electrical field repeating resistance heating strengthening, concrete steps are as follows:
(1) choose the jerusalem artichoke root of fresh mature, after being cleaned by jerusalem artichoke root, be cut into uniform jerusalem artichoke rhizoid with rotary automatic shredding machine;
(2) get 12kg jerusalem artichoke rhizoid and be placed in pulsed electrical field apart from the treatment chamber between two electrodes for 5cm, adding inulin content is that the inulin extracting solution of 10% is to not having all jerusalem artichoke rhizoids, pulsed electrical field repeating resistance heat pre-treatment is carried out by adjustment recurrence interval interval, pulse strength is 600v/cm, pulse duration 50 μ s, the recurrent interval is 100 μ s, has 30 umber of pulses in each recurrence interval, recurrence interval number 100, the recurrence interval is spaced apart 1s;
(3) above-mentioned jerusalem artichoke rhizoid is placed in countercurrent extractor and carries out inulin extraction, Extracting temperature is 50 DEG C, extraction time 70min, and the extracting solution in countercurrent extractor is water, and the mass ratio of extracting solution and jerusalem artichoke rhizoid is 1.4:1.
The inulin extraction yield that the present embodiment obtains is higher by 15% than the inulin extraction yield of traditional extraction technique.
Embodiment 2
Refer to Fig. 1, in the embodiment of the present invention, the method for the auxiliary countercurrent extraction inulin of a kind of pulsed electrical field repeating resistance heating strengthening, concrete steps are as follows:
(1) choose the jerusalem artichoke root of fresh mature, after being cleaned by jerusalem artichoke root, be cut into uniform jerusalem artichoke rhizoid with rotary automatic shredding machine;
(2) get 13kg rhizoid and be placed in pulsed electrical field apart from the treatment chamber between two electrodes for 8m, adding inulin content is that the inulin extracting solution of 15% is to not having all jerusalem artichoke rhizoids, pulsed electrical field repeating resistance heat pre-treatment is carried out by adjustment recurrence interval interval, pulse strength is 800v/cm, pulse duration 70 μ s, the recurrent interval is 150 μ s, has 60 umber of pulses in each recurrence interval, recurrence interval number 80, the recurrence interval is spaced apart 3s;
(3) above-mentioned jerusalem artichoke rhizoid is placed in countercurrent extractor and carries out inulin extraction, Extracting temperature is 30 DEG C, extraction time 90min, and the extracting solution in countercurrent extractor is water, and the mass ratio of extracting solution and jerusalem artichoke rhizoid is 2:1.
The inulin extraction yield that the present embodiment obtains is higher by 5% than the inulin extraction yield of traditional extraction technique.
Embodiment 3
Refer to Fig. 1, in the embodiment of the present invention, the method for the auxiliary countercurrent extraction inulin of a kind of pulsed electrical field repeating resistance heating strengthening, concrete steps are as follows:
(1) choose the jerusalem artichoke root of fresh mature, after being cleaned by jerusalem artichoke root, be cut into uniform jerusalem artichoke rhizoid with rotary automatic shredding machine;
(2) get 14kg rhizoid and be placed in pulsed electrical field apart from the treatment chamber between two electrodes for 10m, add inulin extracting solution to not having all jerusalem artichoke rhizoids, pulsed electrical field repeating resistance heat pre-treatment is carried out by adjustment recurrence interval interval, pulse strength is 1000v/cm, pulse duration 100 μ s, the recurrent interval is 200 μ s, has 100 umber of pulses in each recurrence interval, recurrence interval number 100, the recurrence interval is spaced apart 5s;
(3) above-mentioned jerusalem artichoke rhizoid is placed in countercurrent extractor and carries out inulin extraction, Extracting temperature is 40 DEG C, extraction time 120min, and the extracting solution in countercurrent extractor is water, and the mass ratio of extracting solution and jerusalem artichoke rhizoid is 2.5:1.
The inulin extraction yield that the present embodiment obtains is higher by 10% than the inulin extraction yield of traditional extraction technique.
Compared with prior art, the invention has the beneficial effects as follows:
The present invention has been coupled the electroporation phenomenon of pulsed electrical field and the heat effect of resistive heating, under the synergy of pulsed electrical field and resistive heating, utilize the effectively loose plant cell structures of the high temperature of short period of time, the expansion being more conducive to pulsed electric field electroporation effect realizes, promote the mass transfer of vegetable cell, strengthen inulin from cell to the diffusion extracting solution, reduce the temperature needed for extraction, improve extraction efficiency.
Above the better embodiment of this patent is explained in detail, but this patent is not limited to above-mentioned embodiment, in the ken that one skilled in the relevant art possesses, various change can also be made under the prerequisite not departing from this patent aim.
Claims (5)
1. a method for the auxiliary countercurrent extraction inulin of pulsed electrical field repeating resistance heating strengthening, it is characterized in that, concrete steps are as follows:
(1) choose the jerusalem artichoke root of fresh mature, after being cleaned by jerusalem artichoke root, be cut into uniform jerusalem artichoke rhizoid with rotary automatic shredding machine;
(2) above-mentioned jerusalem artichoke rhizoid is placed in pulsed electrical field apart from the treatment chamber between two electrodes for 5-10cm, add inulin extracting solution to not having all jerusalem artichoke rhizoids, pulsed electrical field repeating resistance heat pre-treatment is carried out by adjustment recurrence interval interval, pulse strength is 500-1000v/cm, pulse duration 50-100 μ s, the recurrent interval is 100-200 μ s, has 30-100 umber of pulse in each recurrence interval, recurrence interval number 5-100, the recurrence interval is spaced apart 1-5s;
(3) above-mentioned jerusalem artichoke rhizoid is placed in countercurrent extractor and carries out inulin extraction, Extracting temperature is 30-80 DEG C, extraction time 60-120min.
2. the method for the auxiliary countercurrent extraction inulin of pulsed electrical field repeating resistance heating strengthening according to claim 1, it is characterized in that, in described inulin extracting solution, inulin is 10-20% according to the content of the content amount percentage ratio of mass percent.
3. the method for the auxiliary countercurrent extraction inulin of pulsed electrical field repeating resistance heating strengthening according to claim 1, it is characterized in that, the extraction agent in described countercurrent extractor is water, and the mass ratio of extraction agent and jerusalem artichoke rhizoid is 1.2-2.5:1.
4. the method for the auxiliary countercurrent extraction inulin of pulsed electrical field repeating resistance heating strengthening according to claim 1, it is characterized in that, described electrode adopts plate electrode.
5. the method for the auxiliary countercurrent extraction inulin of pulsed electrical field repeating resistance heating strengthening according to claim 1,4, it is characterized in that, the material of described electrode is iron, copper or aluminium.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107261549A (en) * | 2017-07-19 | 2017-10-20 | 宁波检验检疫科学技术研究院 | Plant catalyst technology is extracted in a kind of electromagnetic wave and electric pulse combination extraction apparatus and electromagnetic wave and electric pulse combination |
| CN107261548A (en) * | 2017-07-19 | 2017-10-20 | 宁波检验检疫科学技术研究院 | Plant catalyst technology and electromagnetic wave and electric pulse combination extraction element are extracted in a kind of electromagnetic wave and electric pulse combination |
| CN107586715A (en) * | 2017-10-09 | 2018-01-16 | 复旦大学 | Utilize the apparatus and method of electroporation technology extraction Chinese medicine drug ingedient |
| CN111296728A (en) * | 2020-04-10 | 2020-06-19 | 武汉轻工大学 | Inulin compound induced by pulsed electric field and preparation method thereof |
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| CN102898548A (en) * | 2012-09-19 | 2013-01-30 | 中国科学院烟台海岸带研究所 | Method for preparing inulin |
| CN103223261A (en) * | 2013-04-22 | 2013-07-31 | 华南理工大学 | Apparatus and method for pulsed electric field-assisted countercurrent extraction of grape skin residue polyphenol |
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2015
- 2015-01-07 CN CN201510006439.XA patent/CN104558256A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102898548A (en) * | 2012-09-19 | 2013-01-30 | 中国科学院烟台海岸带研究所 | Method for preparing inulin |
| CN103223261A (en) * | 2013-04-22 | 2013-07-31 | 华南理工大学 | Apparatus and method for pulsed electric field-assisted countercurrent extraction of grape skin residue polyphenol |
Non-Patent Citations (3)
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| K.V.LOGINOVA 等: "Acceleration of soluble matter extraction from chicory with pulsed electric fields", 《JOURNAL OF FOOD ENGINEERING》 * |
| ZHENZHOU ZHU 等: "Better damage of chicory tissue by combined electroporation and ohmic heating for solute extraction", 《FOOD AND BIOPRODUCTS PROCESSING》 * |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107261549A (en) * | 2017-07-19 | 2017-10-20 | 宁波检验检疫科学技术研究院 | Plant catalyst technology is extracted in a kind of electromagnetic wave and electric pulse combination extraction apparatus and electromagnetic wave and electric pulse combination |
| CN107261548A (en) * | 2017-07-19 | 2017-10-20 | 宁波检验检疫科学技术研究院 | Plant catalyst technology and electromagnetic wave and electric pulse combination extraction element are extracted in a kind of electromagnetic wave and electric pulse combination |
| CN107586715A (en) * | 2017-10-09 | 2018-01-16 | 复旦大学 | Utilize the apparatus and method of electroporation technology extraction Chinese medicine drug ingedient |
| CN111296728A (en) * | 2020-04-10 | 2020-06-19 | 武汉轻工大学 | Inulin compound induced by pulsed electric field and preparation method thereof |
| CN111296728B (en) * | 2020-04-10 | 2023-03-07 | 武汉轻工大学 | Inulin compound induced by pulsed electric field and preparation method thereof |
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Application publication date: 20150429 |