CN104547001A - Process for extracting anti-osteoporosis and lipid-decreasing substances from pigeon pea leaves - Google Patents
Process for extracting anti-osteoporosis and lipid-decreasing substances from pigeon pea leaves Download PDFInfo
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- CN104547001A CN104547001A CN201510021698.XA CN201510021698A CN104547001A CN 104547001 A CN104547001 A CN 104547001A CN 201510021698 A CN201510021698 A CN 201510021698A CN 104547001 A CN104547001 A CN 104547001A
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Abstract
The invention relates to a process for extracting anti-osteoporosis and lipid-decreasing substances from pigeon pea leaves. The process comprises the steps of carrying out coarse powder grinding, percolation, macroporous resin adsorption, reduced pressure condensation, low-temperature precipitation, elution and low-temperature crystallization on dried pigeon pea leaves, so as to obtain the anti-osteoporosis and lipid-decreasing substances. According to the process, the percolation extraction is carried out, and heating is omitted, so that the damage to effective components is low; macroporous resin columns DM21/D41 are combined for adsorbing the different components of the pigeon pea leaves, so that the process is simple, the yield is high, extracted effective parts are toxic free, and the substances have remarkable anti-osteoporosis and lipid-decreasing effects.
Description
Technical field
The invention belongs to pharmaceutical technology sectors, be specifically related to a kind of extraction process, particularly relate to a kind of technique extracting osteoporosis and blood fat reducing thing from Folium Cajani.
Background technology
Osteoporosis is impaired with osseous tissue microstructure, bone ore deposit composition and bone matrix equal proportion ground constantly reduce, sclerotin is thinning, bone trabecula quantity reduces, the disease of a kind of general metabolism obstacle that bone fragility increases and risk of fractures degree raises, it is a kind of commonly encountered diseases of postmenopausal women and old people, a large amount of research data shows in addition, with advancing age, function of human body more or less occurs that Progressive symmetric erythrokeratodermia goes down, under endocrine hormone impact, there is redistribution in body fat, the prevalence of China's middle-aged and elderly people hyperlipemia has the trend increased year by year, although countries in the world have carried out studying extensively and profoundly to hyperlipemia and osteoporosis, but still lack efficient, the clinical application of safe and applicable China morbidity feature.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art and provide that a kind of raw material sources are extensive, cheap, extraction process is simple, osteoporosis is evident in efficacy, there is the Folium Cajani extraction process of effect for reducing fat.
Technical solution of the present invention is: a kind of technique extracting osteoporosis and blood fat reducing thing from Folium Cajani, described extraction process comprises the steps:
Step 1: selection: choose a certain amount of dry Folium Cajani;
Step 2: coarse powder: Folium Cajani step 1 obtained is put into pulverizer and pulverized, the granule of pulverizing is particle diameter 20-60 order;
Step 3: percolation: coarse powder particles step 2 obtained puts into percolator, airtight placement 2 hours, is then taken as 80% ethanol percolation of Folium Cajani 5 times of quality, flow speed control 10-20ml/min;
Step 4: process DM21: get 4% sodium hydroxide solution, 4% hydrochloric acid solution, ethanol alternate treatment successively that volume is DM21 volume 2 times respectively;
Step 5: macroporous resin adsorption: percolate step 3 obtained slowly adds to the DM21 macroporous resin column handled well, flow speed control 10-20ml/min;
Step 6: concentrating under reduced pressure: liquid step 5 obtained, at 60 DEG C, concentrates the quality of most Folium Cajani amount half under the environment of pressure 0.04-0.1mpa;
Step 7: low-temperature precipitation: concentrated solution step 6 obtained is placed to and occurs a large amount of white precipitate at 0-10 DEG C;
Step 8: eluting: the 90% ethanol elution DM21 resin column being taken as Folium Cajani quality 2 times;
Step 9; Reclaim ethanol: put into by the eluent in step 8 in decompression tank, control temperature is 60 DEG C, pressure 0.04-0.1mpa, and heating recovery ethanol is not until have alcohol taste;
Step 10: process D941: getting volume is that 4% sodium hydroxide solution of D941 volume 2 times passes through at 10-30ml/min flow velocity, deionized water is washed till effluent neutrality, getting volume is again that 4% hydrochloric acid solution of D941 volume 2 times passes through at 10-30ml/min flow velocity, deionized water is washed till effluent neutrality, get 95% ethanol elution that volume is D941 volume 1 times again, finally get the deionization washing that volume is D941 volume 3 times;
Step 11: water elution: step 9 is obtained liquid and flow into D941 macroporous adsorptive resins with flow velocity 20-30ml/min, then water elution to effluent is colourless, discards aqueous solution;
Step 12:70% ethanol elution: the D941 macroporous adsorptive resins of step 11 is continued 70% ethanol elution being incorporated as Folium Cajani quality 2 times, collects eluent;
Step 13: concentrated: being put by the eluent obtained of step 12 to thin film concentration device in temperature is 60 DEG C, pressure 0.04-0.1mpa, the quality of concentrated most Folium Cajani amount 1/5;
Step 14: low temperature crystallization: concentrated solution step 13 obtained is placed 12 hours at low temperature 0-4 DEG C, occurs white crystal.
Described DM21 macroporous resin column is diameter 8cm, high 150cm.
Described D941 macroporous resin column is diameter 8cm, high 150cm.
The present invention has the following advantages: it is through coarse powder by the Folium Cajani of drying, percolation, macroporous resin adsorption, concentrating under reduced pressure, low-temperature precipitation, eluting, the osteoporosis obtained after concentrating under reduced pressure and low temperature crystallization and blood fat reducing thing, the present invention passes through seepage pressure effects, without heating, effective ingredient destroys less, by macroporous resin DM21/D941 conbined usage and the volume of DM21 with D941 is identical, adsorb the heterogeneity in Folium Cajani, the white precipitate obtained and white crystal are different material, but all there is the function of osteoporosis and blood fat reducing, whole technique is simple, yield is high, the effective site avirulence extracted, its osteoporosis, lipid-lowering effect is remarkable, to have raw material sources extensive in the present invention in a word, cheap, extraction process is simple, osteoporosis is evident in efficacy, there is the advantage of effect for reducing fat.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated.
Embodiment 1
From Folium Cajani, extract a technique for osteoporosis and blood fat reducing thing, described extraction process comprises the steps:
Step 1: selection: choose 100 kilograms of dry Folium Cajanis;
Step 2: coarse powder: Folium Cajani step 1 obtained is put into pulverizer and pulverized, the granule of pulverizing is particle diameter 20-60 order;
Step 3: percolation: coarse powder particles step 2 obtained puts into percolator, airtight placement 2 hours, then gets 500 kilogram of 80% ethanol percolation, flow speed control 10-20ml/min;
Step 4: process DM21: get 4% sodium hydroxide solution, 4% hydrochloric acid solution, ethanol alternate treatment successively that volume is DM21 volume 2 times respectively;
Step 5: macroporous resin adsorption: percolate step 3 obtained slowly adds to the DM21 macroporous resin column handled well, flow speed control 10-20ml/min;
Step 6: concentrating under reduced pressure: liquid step 5 obtained, at 60 DEG C, concentrates most 50 kilograms under the environment of pressure 0.04-0.1mpa;
Step 7: low-temperature precipitation: concentrated solution step 6 obtained is placed to and occurs a large amount of white precipitate at 0-10 DEG C;
Step 8: eluting: the 90% ethanol elution DM21 resin column getting 200 kilograms;
Step 9: reclaim ethanol: put into by the eluent in step 8 in decompression tank, control temperature is 60 DEG C, pressure 0.04-0.1mpa, and heating recovery ethanol is not until have alcohol taste;
Step 10: process D941: getting volume is that 4% sodium hydroxide solution of D941 volume 2 times passes through at 10-30ml/min flow velocity, deionized water is washed till effluent neutrality, getting volume is again that 4% hydrochloric acid solution of D941 volume 2 times passes through at 10-30ml/min flow velocity, deionized water is washed till effluent neutrality, get 95% ethanol elution that volume is D941 volume 1 times again, finally get the deionization washing that volume is D941 volume 3 times;
Step 11: water elution: step 9 is obtained liquid and flow into D941 macroporous adsorptive resins with flow velocity 20-30ml/min, then water elution to effluent is colourless, discards aqueous solution;
Step 12:70% ethanol elution: the D941 macroporous adsorptive resins of step 11 is continued 70% ethanol elution adding 200 kilograms, collects eluent;
Step 13: concentrated: being put by the eluent obtained of step 12 to thin film concentration device in temperature is 60 DEG C, and pressure 0.04-0.1mpa, is concentrated into 20 kilograms;
Step 14: low temperature crystallization: concentrated solution step 13 obtained is placed 12 hours at low temperature 0-4 DEG C, occurs white crystal.
The present invention has the following advantages: it is through coarse powder by the Folium Cajani of drying, percolation, macroporous resin adsorption, concentrating under reduced pressure, low-temperature precipitation, eluting, the osteoporosis obtained after concentrating under reduced pressure and low temperature crystallization and blood fat reducing thing, first Folium Cajani is pulverized by the present invention, in order in the process of percolation, can fully contact with ethanol, when percolation, airtight placement 2 hours, in order to ensure that Folium Cajani can be fully moistening, then macroporous resin adsorption is passed through, obtain white precipitate, there is the function of osteoporosis and blood fat reducing, whole process is without heating, effective ingredient destroys less, just macroporous resin DM21/D941 conbined usage again in addition, adsorb the heterogeneity in Folium Cajani, obtain white crystal, also there is the function of osteoporosis and blood fat reducing, whole technique is simple, yield is high, the effective site avirulence extracted, its osteoporosis, lipid-lowering effect is remarkable, to have raw material sources extensive in the present invention in a word, cheap, extraction process is simple, osteoporosis is evident in efficacy, there is the advantage of effect for reducing fat.
Embodiment 2
From Folium Cajani, extract a technique for osteoporosis and blood fat reducing thing, described extraction process comprises the steps:
Step 1: selection: choose 50 kilograms of dry Folium Cajanis;
Step 2: coarse powder: Folium Cajani step 1 obtained is put into pulverizer and pulverized, the granule of pulverizing is particle diameter 20-60 order;
Step 3: percolation: coarse powder particles step 2 obtained puts into percolator, airtight placement 2 hours, is then taken as 80% ethanol percolation of Folium Cajani 5 times of quality, flow speed control 10-20ml/min;
Step 4: process DM21: get 4% sodium hydroxide solution, 4% hydrochloric acid solution, ethanol alternate treatment successively that volume is DM21 volume 2 times respectively;
Step 5: macroporous resin adsorption: percolate step 3 obtained slowly adds to the DM21 macroporous resin column handled well, flow speed control 10-20ml/min;
Step 6: concentrating under reduced pressure: liquid step 5 obtained, at 60 DEG C, is concentrated into 25 kilograms under the environment of pressure 0.04-0.1mpa;
Step 7: low-temperature precipitation: concentrated solution step 6 obtained is placed to and occurs a large amount of white precipitate at 0-10 DEG C;
Step 8: eluting: the 90% ethanol elution DM21 resin column getting 100 kilograms;
Step 9: reclaim ethanol: put into by the eluent in step 8 in decompression tank, control temperature is 60 DEG C, pressure 0.04-0.1mpa, and heating recovery ethanol is not until have alcohol taste;
Step 10: process D941: getting volume is that 4% sodium hydroxide solution of D941 volume 2 times passes through at 10-30ml/min flow velocity, deionized water is washed till effluent neutrality, getting volume is again that 4% hydrochloric acid solution of D941 volume 2 times passes through at 10-30ml/min flow velocity, deionized water is washed till effluent neutrality, get 95% ethanol elution that volume is D941 volume 1 times again, finally get the deionization washing that volume is D941 volume 3 times;
Step 11: water elution: step 9 is obtained liquid and flow into D941 macroporous adsorptive resins with flow velocity 20-30ml/min, then water elution to effluent is colourless, discards aqueous solution;
Step 12:70% ethanol elution: the D941 macroporous adsorptive resins of step 11 is continued 70% ethanol elution adding 100 kilograms, collects eluent;
Step 13: concentrated: being put by the eluent obtained of step 12 to thin film concentration device in temperature is 60 DEG C, and pressure 0.04-0.1mpa, is concentrated into 10 kilograms;
Step 14: low temperature crystallization: concentrated solution step 13 obtained is placed 12 hours at low temperature 0-4 DEG C, occurs white crystal.
The present invention has the following advantages: it is through coarse powder by the Folium Cajani of drying, percolation, macroporous resin adsorption, concentrating under reduced pressure, low-temperature precipitation, eluting, the osteoporosis obtained after concentrating under reduced pressure and low temperature crystallization and blood fat reducing thing, first Folium Cajani is pulverized by the present invention, in order in the process of percolation, can fully contact with ethanol, when percolation, airtight placement 2 hours, in order to ensure that Folium Cajani can be fully moistening, then macroporous resin adsorption is passed through, obtain white precipitate, there is the function of osteoporosis and blood fat reducing, whole process is without heating, effective ingredient destroys less, just macroporous resin DM21/D941 conbined usage again in addition, adsorb the heterogeneity in Folium Cajani, obtain white crystal, also there is the function of osteoporosis and blood fat reducing, whole technique is simple, yield is high, the effective site avirulence extracted, its osteoporosis, lipid-lowering effect is remarkable, to have raw material sources extensive in the present invention in a word, cheap, extraction process is simple, osteoporosis is evident in efficacy, there is the advantage of effect for reducing fat.
Embodiment 3
From Folium Cajani, extract a technique for osteoporosis and blood fat reducing thing, described extraction process comprises the steps:
Step 1: selection: the dry Folium Cajani choosing 1000 kilograms;
Step 2: coarse powder: Folium Cajani step 1 obtained is put into pulverizer and pulverized, the granule of pulverizing is particle diameter 20-60 order;
Step 3: percolation: coarse powder particles step 2 obtained puts into percolator, airtight placement 2 hours, then gets 80% ethanol percolation of 5000 kilograms, flow speed control 10-20ml/min;
Step 4: process DM21: get 4% sodium hydroxide solution, 4% hydrochloric acid solution, ethanol alternate treatment successively that volume is DM21 volume 2 times respectively;
Step 5: macroporous resin adsorption: percolate step 3 obtained slowly adds to the DM21 macroporous resin column handled well, flow speed control 10-20ml/min;
Step 6: concentrating under reduced pressure: liquid step 5 obtained, at 60 DEG C, is concentrated into 500 kilograms under the environment of pressure 0.04-0.1mpa;
Step 7: low-temperature precipitation: concentrated solution step 6 obtained is placed to and occurs a large amount of white precipitate at 0-10 DEG C;
Step 8: eluting: the 90% ethanol elution DM21 resin column getting 2000 kilograms;
Step 9: reclaim ethanol: put into by the eluent in step 8 in decompression tank, control temperature is 60 DEG C, pressure 0.04-0.1mpa, and heating recovery ethanol is not until have alcohol taste;
Step 10: process D941: getting volume is that 4% sodium hydroxide solution of D941 volume 2 times passes through at 10-30ml/min flow velocity, deionized water is washed till effluent neutrality, getting volume is again that 4% hydrochloric acid solution of D941 volume 2 times passes through at 10-30ml/min flow velocity, deionized water is washed till effluent neutrality, get 95% ethanol elution that volume is D941 volume 1 times again, finally get the deionization washing that volume is D941 volume 3 times;
Step 11: water elution: step 9 is obtained liquid and flow into D941 macroporous adsorptive resins with flow velocity 20-30ml/min, then water elution to effluent is colourless, discards aqueous solution;
Step 12:70% ethanol elution: the D941 macroporous adsorptive resins of step 11 is continued 70% ethanol elution adding 2000 kilograms, collects eluent;
Step 13: concentrated: being put by the eluent obtained of step 12 to thin film concentration device in temperature is 60 DEG C, and pressure 0.04-0.1mpa, is concentrated into 200 kilograms;
Step 14: low temperature crystallization: concentrated solution step 13 obtained is placed 12 hours at low temperature 0-4 DEG C, occurs white crystal.
The present invention has the following advantages: it is through coarse powder by the Folium Cajani of drying, percolation, macroporous resin adsorption, concentrating under reduced pressure, low-temperature precipitation, eluting, the osteoporosis obtained after concentrating under reduced pressure and low temperature crystallization and blood fat reducing thing, first Folium Cajani is pulverized by the present invention, in order in the process of percolation, can fully contact with ethanol, when percolation, airtight placement 2 hours, in order to ensure that Folium Cajani can be fully moistening, then macroporous resin adsorption is passed through, obtain white precipitate, there is the function of osteoporosis and blood fat reducing, whole process is without heating, effective ingredient destroys less, just macroporous resin DM21/D941 conbined usage again in addition, adsorb the heterogeneity in Folium Cajani, obtain white crystal, also there is the function of osteoporosis and blood fat reducing, whole technique is simple, yield is high, the effective site avirulence extracted, its osteoporosis, lipid-lowering effect is remarkable, to have raw material sources extensive in the present invention in a word, cheap, extraction process is simple, osteoporosis is evident in efficacy, there is the advantage of effect for reducing fat.
Claims (3)
1. from Folium Cajani, extract a technique for osteoporosis and blood fat reducing thing, it is characterized in that: described extraction process comprises the steps:
Step 1: selection: choose a certain amount of dry Folium Cajani;
Step 2: coarse powder: Folium Cajani step 1 obtained is put into pulverizer and pulverized, the granule of pulverizing is particle diameter 20-60 order;
Step 3: percolation: coarse powder particles step 2 obtained puts into percolator, airtight placement 2 hours, is then taken as 80% ethanol percolation of Folium Cajani 5 times of quality, flow speed control 10-20ml/min;
Step 4: process DM21: get 4% sodium hydroxide solution, 4% hydrochloric acid solution, ethanol alternate treatment successively that volume is DM21 volume 2 times respectively;
Step 5: macroporous resin adsorption: percolate step 3 obtained slowly adds to the DM21 macroporous resin column handled well, flow speed control 10-20ml/min;
Step 6: concentrating under reduced pressure: liquid step 5 obtained, at 60 DEG C, concentrates the quality of most Folium Cajani amount half under the environment of pressure 0.04-0.1mpa;
Step 7: low-temperature precipitation: concentrated solution step 6 obtained is placed to and occurs a large amount of white precipitate at 0-10 DEG C;
Step 8: eluting: the 90% ethanol elution DM21 resin column being taken as Folium Cajani quality 2 times;
Step 9: reclaim ethanol: put into by the eluent in step 8 in decompression tank, control temperature is 60 DEG C, pressure 0.04-0.1mpa, and heating recovery ethanol is not until have alcohol taste;
Step 10: process D941: getting volume is that 4% sodium hydroxide solution of D941 volume 2 times passes through at 10-30ml/min flow velocity, deionized water is washed till effluent neutrality, getting volume is again that 4% hydrochloric acid solution of D941 volume 2 times passes through at 10-30ml/min flow velocity, deionized water is washed till effluent neutrality, get 95% ethanol elution that volume is D941 volume 1 times again, finally get the deionization washing that volume is D941 volume 3 times;
Step 11: water elution: step 9 is obtained liquid and flow into D941 macroporous adsorptive resins with flow velocity 20-30ml/min, then water elution to effluent is colourless, discards aqueous solution;
Step 12:70% ethanol elution: the D941 macroporous adsorptive resins of step 11 is continued 70% ethanol elution being incorporated as Folium Cajani quality 2 times, collects eluent;
Step 13: concentrated: being put by the eluent obtained of step 12 to thin film concentration device in temperature is 60 DEG C, pressure 0.04-0.1mpa, the quality of concentrated most Folium Cajani amount 1/5;
Step 14: low temperature crystallization: concentrated solution step 13 obtained is placed 12 hours at low temperature 0-4 DEG C, occurs white crystal.
2. a kind of technique extracting osteoporosis and blood fat reducing thing from Folium Cajani as claimed in claim 1, is characterized in that: described DM21 macroporous resin column is diameter 8cm, high 150cm.
3. a kind of technique extracting osteoporosis and blood fat reducing thing from Folium Cajani as claimed in claim 1, is characterized in that: described D941 macroporous resin column is diameter 8cm, high 150cm.
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| CN201510021698.XA CN104547001A (en) | 2015-01-09 | 2015-01-09 | Process for extracting anti-osteoporosis and lipid-decreasing substances from pigeon pea leaves |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107879906A (en) * | 2018-01-16 | 2018-04-06 | 河南省中医药研究院 | Extract cajanin, Longistylin A, Longistylin C, Cajanuslactone A technique |
| CN108096369A (en) * | 2018-01-16 | 2018-06-01 | 河南省中医药研究院 | A kind of trophic nerve cell, the extraction process of anti-apolexis composition |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020055541A1 (en) * | 2000-08-16 | 2002-05-09 | Shaman Pharmaceuticals, Inc. | Compositions containing hypotriglyceridemically active stilbenoids |
| CN102755309A (en) * | 2012-06-19 | 2012-10-31 | 暨南大学 | Application of cajanin to preparation of medicament for treating or preventing caput femoris necrosis |
-
2015
- 2015-01-09 CN CN201510021698.XA patent/CN104547001A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020055541A1 (en) * | 2000-08-16 | 2002-05-09 | Shaman Pharmaceuticals, Inc. | Compositions containing hypotriglyceridemically active stilbenoids |
| CN102755309A (en) * | 2012-06-19 | 2012-10-31 | 暨南大学 | Application of cajanin to preparation of medicament for treating or preventing caput femoris necrosis |
Non-Patent Citations (1)
| Title |
|---|
| 梅全喜: "《广州地产药材研究》", 31 May 2011 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107879906A (en) * | 2018-01-16 | 2018-04-06 | 河南省中医药研究院 | Extract cajanin, Longistylin A, Longistylin C, Cajanuslactone A technique |
| CN108096369A (en) * | 2018-01-16 | 2018-06-01 | 河南省中医药研究院 | A kind of trophic nerve cell, the extraction process of anti-apolexis composition |
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