CN104546964A - Traditional Chinese medicine quantification standard method - Google Patents
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/027—Liquid chromatography
Abstract
The invention belongs to the field of traditional Chinese medicine quantification standards and in particular relates to a traditional Chinese medicine quantification standard method. The method comprises the following steps: extracting and separating traditional Chinese medicines, thereby obtaining pure traditional Chinese medicines of different traditional Chinese medicine types; detecting the pure traditional Chinese medicines by adopting high performance liquid chromatography, so that the purity of the pure traditional Chinese medicines is over 99 percent; and taking the pure traditional Chinese medicines of different types as the traditional Chinese medicine quantification standard, weighing and dosing. The pure traditional Chinese medicine disclosed by the invention refers to a set of total-effect ingredients of traditional Chinese medicinal materials, the purity is 99 percent, the difference of production places and varieties is avoided, and the pure traditional Chinese medicine can be stably and efficiently applied to the traditional Chinese medicine formula. The mathematical quantification method for the pure traditional Chinese medicine scientifically solves the formula amount, so that the expected effect is achieved, the quality standard data are provided for traditional Chinese medicine quantification, the pure traditional Chinese medicine is an outstanding representative of traditional Chinese medicine quantification transformation standard, the applied traditional Chinese medicine is quantified by using the standard data, and the traditional Chinese medicine has the advantages of high quality, high efficiency, safety, stability, controllable quality and convenience in administration.
Description
Technical field
The invention belongs to Chinese medicine quantitative criteria field, particularly relate to a kind of method of Chinese medicine quantitative criteria.
Background technology
1996, the Department of Science and Technology, State Administration of Traditional Chinese Medicine have carried out " modernization of Chinese medicine development strategy " subject study, indicate that China's " modernization of Chinese medicine " formally proposes, from then on relevant with Chinese medicine department and industry are all paid much attention to, actively throwing oneself into the practice of the modernization of Chinese medicine, there is the upsurge of the modernization of Chinese medicine in the whole nation.Due to the existence of the various disorders factors such as the standardized related data shortage of Chinese medicine, develop comparatively slow.The method adopting traditional decocting medicine always and eat soup taken by Chinese medicine, Chinese medicine extraction is become grain shape by large corporation with good conditionsi, but the rough extraction of the many employings of preparation concentrates, there is no unified processing technology and the standardized data of purity, cause preparation drug effect low, the property of medicine is stable poor, seriously constrains the development of China's Chinese medicine and pharmacy.Therefore, the distance that the transformation of systematic analysis Chinese medicine is passed by, sums up the current situation and problem of the modernization of Chinese medicine, and improving Chinese crude drug, to quantize transformation standard be a very urgent task.
With regard to human health development, Chinese medicine safety in diagnosis and treatment is high, and side effect is little, and table is originally double to be controlled.And domestic Chinese medicine instructions of taking is complicated, quantizing transformation does not have relevant criterion, causes Chinese medicine to quantize transformation uneven.A kind of method proposing Chinese medicine quantitative criteria is very urgent, and what Chinese medicine was pure appears as Chinese medicine quantification transformation provides gross data.The application that Chinese medicine is pure is with the full effect composition in the methods analyst Chinese crude drug of guantification, for science, accurate medication provide data basis.The pure proposition of Chinese medicine for a change this present situation provides powerful guarantee, for Traditional Chinese Medicine dosage provides the data standard of science.
Summary of the invention
It is change the conventional fabrication method of Chinese medicine that Chinese medicine quantizes transformation, overcomes that boiling medicine of existing in application in TCM is slow, the medical science such as difficult of eating soup covers disease.Chinese medicine is pure is the novel medical material that Chinese medicine quantizes transformation, is the pure full effect composition of Chinese crude drug, can be used as a kind of monomer Chinese crude drug of Chinese patent medicine.There is consumption strong, the advantages such as drug effect is high, and stability is strong.Chinese medicine is pure provides standard for Chinese medicine quantizes transformation, the pure usage variance can effectively avoided because the Chinese crude drug place of production, kind difference produce of Chinese medicine, the purity that Chinese medicine is pure is that Chinese medicine material imitates 99% of composition entirely, there is no kind and place of production difference, for unified Traditional Chinese Medicine dosage provides gross data, for Chinese medicine marches toward international market establish a firm foundation.
The problem to be solved in the present invention how to overcome the deficiencies in the prior art, provides a kind of method of Chinese medicine quantitative criteria.The scheme that the present invention adopts is: a kind of method of Chinese medicine quantitative criteria, Chinese medicine being carried out extraction and isolation, to obtain the Chinese medicine of different Chinese medicine kind pure, adopt efficient Liquid Detection Chinese medicine pure, ensure that Chinese medicine purity is more than 99%, carries out weighing administration so that different types of Chinese medicine is pure as Chinese medicine quantitative criteria.
Present invention also offers the pure preparation method of described Chinese medicine: the first step, get quantitative Chinese crude drug, add the ethanol of 1-5 times amount, at 55-85 DEG C of alcoholization 30-90 minute, then add the water of 1-5 times amount, at 100 DEG C of water-soluble 30-90 minute, reclaim ethanol, obtain mixed liquor, filter mixed liquor obtains filtrate, and the Chinese medicine residue of filtration repeats above extraction step; Second step, merges twice filtrate, goes out large particulate matter in filtrate and supernatant with microporous filter membrane or Ultra filtration membrane, and wherein large particulate matter repeats to extract as Chinese medicine residue, and supernatant concentration, spraying are dried Chinese medicine is pure.
Present invention also offers the pure preparation method of described Chinese medicine: the first step, get quantitative Chinese crude drug, add the distilled water of 1-5 times amount, decoct 30-90 minute at 55-85 DEG C, then add the distilled water of 1-5 times amount, at 100 DEG C of water-soluble 30-90 minute, distilling under reduced pressure, obtain the mixed liquor concentrated, filter mixed liquor obtains filtrate, and the Chinese medicine residue of filtration repeats above extraction step; Second step, merges twice filtrate, goes out large particulate matter in filtrate and supernatant with microporous filter membrane or Ultra filtration membrane, and wherein large particulate matter repeats to extract as Chinese medicine residue, and supernatant concentration, spraying are dried Chinese medicine is pure.
The pure detection of Chinese medicine of the present invention adopts efficient Liquid Detection.
Ultrafilter membrane molecular cut off of the present invention is 1K-300KDalton.
The invention has the advantages that: Chinese medicine is pure is the set that all kinds of Chinese crude drug imitates composition entirely, and its high purity 99%, do not have the place of production and breed difference, Absorbable organic halogens is applicable to Chinese medicine formula efficiently.Chinese medicine is pure is the new medicine material that Chinese crude drug quantizes transformation, has the advantages such as high-quality, efficient, safe, stable, quality controllable, taking convenience.The methodological science of the pure guantification of Chinese medicine solves formula consumption, thus realizes effect of expection.The method of a kind of Chinese medicine quantitative criteria of the present invention, quality standard data are provided for Chinese medicine quantizes transformation, Chinese medicine is pure is that Chinese medicine quantizes the outstanding representative of transformation standard, quantizes application Chinese medicine with normal data, has high-quality, efficient, safe, stable, quality controllable, taking convenience etc.
Detailed description of the invention
" Chinese medicine is pure " is prerequisite and the object of Chinese medicine quantitative criteria, the pure deviation fundamentally solving Chinese crude drug consumption and produce because of kind and place of production difference of Chinese medicine.Such as: the average withdrawal ratio that Radix Angelicae Sinensis is pure is 16%, the average purification ratio of Radix Et Caulis Acanthopanacis Senticosi is 4%; For another example, the average withdrawal ratio that Sichuan Radix Angelicae Sinensis is pure is 17%, and the average withdrawal ratio that Angelica Sinensis is pure is 15%.Chinese medicine is pure is the set that all kinds of Chinese crude drug imitates composition entirely, and its high purity 99%, do not have the place of production and breed difference, Absorbable organic halogens is applicable to Chinese medicine formula efficiently.Chinese medicine is pure is the new medicine material that Chinese crude drug quantizes transformation, has the advantages such as high-quality, efficient, safe, stable, quality controllable, taking convenience.The methodological science of the pure guantification of Chinese medicine solves formula consumption, thus realizes effect of expection.
Embodiment 1
Pure for example with Radix Angelicae Sinensis Chinese medicine, first extract Radix Angelicae Sinensis Chinese medicine pure, the first step, gets a certain amount of Radix Angelicae Sinensis, add the ethanol of 1-5 times amount, at 55-85 DEG C of alcoholization 30-90 minute, then add the water of 1-5 times amount, at 100 DEG C of water-soluble 30-90 minute, reclaim ethanol, obtain mixed liquor, filter mixed liquor obtains filtrate, and the Radix Angelicae Sinensis residue of filtration repeats above extraction step; Second step, merge twice filtrate, use aperture 1000um, molecular cut off is that 300KDalton microporous filter membrane isolates large particulate matter in filtrate and supernatant, wherein large particulate matter repeats to extract as Chinese medicine residue, and supernatant concentration, spraying are dried Radix Angelicae Sinensis Chinese medicine is pure.Adopt that high-efficient liquid is pure relative to Radix Angelicae Sinensis Chinese medicine detects, ensure purity more than 99%.
According to Chinese medicine formula, calculate the content of Radix Angelicae Sinensis in Chinese medicine formula, the average purification ratio inverse according to Chinese medicine angelica goes out the pure consumption of required Radix Angelicae Sinensis Chinese medicine, directly take the consumption that above-mentioned Radix Angelicae Sinensis Chinese medicine is pure, time saving and energy saving, avoid extraction step, time saving and energy saving.
Embodiment 2
Pure for example with Radix Et Caulis Acanthopanacis Senticosi Chinese medicine, first extract Radix Et Caulis Acanthopanacis Senticosi Chinese medicine pure, the first step, gets a certain amount of Radix Et Caulis Acanthopanacis Senticosi, add the distilled water of 1-5 times amount, decoct 30-90 minute at 55-85 DEG C, then add the water of 1-5 times amount, at 100 DEG C of water-soluble 30-90 minute, distilling under reduced pressure, obtain mixed liquor, filter mixed liquor obtains filtrate, and the Radix Et Caulis Acanthopanacis Senticosi residue of filtration repeats above extraction step; Second step, merge twice filtrate, use aperture 0.2um, molecular cut off is that 150KDalton nanofiltration membrane separation goes out large particulate matter in filtrate and supernatant, wherein large particulate matter repeats to extract as Radix Et Caulis Acanthopanacis Senticosi residue, and supernatant concentration, spraying are dried Radix Et Caulis Acanthopanacis Senticosi Chinese medicine is pure.Adopt that high-efficient liquid is pure relative to Radix Et Caulis Acanthopanacis Senticosi Chinese medicine detects, ensure purity more than 99%.
According to suitable Chinese medicine formula, calculate the content of Radix Et Caulis Acanthopanacis Senticosi in Chinese medicine formula, the average purification ratio inverse according to Chinese medicine Radix Et Caulis Acanthopanacis Senticosi goes out the pure consumption of required Radix Et Caulis Acanthopanacis Senticosi Chinese medicine, directly take the consumption that above-mentioned Radix Et Caulis Acanthopanacis Senticosi Chinese medicine is pure, time saving and energy saving, avoid extraction step, time saving and energy saving.
Embodiment 3
Pure for example with Radix Schisandrae Bicoloris Chinese medicine, first extract Radix Schisandrae Bicoloris Chinese medicine pure, the first step, gets a certain amount of Radix Schisandrae Bicoloris, add the distilled water of 5 times amount, decoct 120 minutes at 100 DEG C, then add the water of 3 times amount, water-soluble 60 minutes at 100 DEG C, distilling under reduced pressure, obtain mixed liquor, filter mixed liquor obtains filtrate, and the Radix Schisandrae Bicoloris residue of filtration repeats above extraction step; Second step, merge twice filtrate, by high temperature resistant high rejection rolling NF membrane, tolerable temperature 50-90 DEG C, aperture is at 1000um, and molecular cut off, at 300KDalton, isolates the large particulate matter in filtrate and supernatant, wherein large particulate matter repeats to extract as Radix Schisandrae Bicoloris residue, and supernatant concentration, spraying are dried Radix Schisandrae Bicoloris Chinese medicine is pure.Adopt that high-efficient liquid is pure relative to Radix Schisandrae Bicoloris Chinese medicine detects, ensure purity more than 99%.
According to suitable Chinese medicine formula, calculate the content of Radix Schisandrae Bicoloris in Chinese medicine formula, the average purification ratio inverse according to Chinese medicine Radix Schisandrae Bicoloris goes out the pure consumption of required Radix Schisandrae Bicoloris Chinese medicine, directly takes the consumption that above-mentioned Radix Schisandrae Bicoloris Chinese medicine is pure, avoid extraction step, time saving and energy saving.
Embodiment 4
Pure for example with Radix Isatidis Chinese medicine, first Radix Isatidis Chinese medicine is extracted pure, the first step, gets a certain amount of Radix Isatidis, adds the dehydrated alcohol of 10 times amount, decoct 100 minutes at 100 DEG C, distilling under reduced pressure, goes out ethanol, obtains mixed liquor, filter mixed liquor obtains filtrate, and the Radix Isatidis residue of filtration repeats above extraction step; Second step, merge twice filtrate, by high temperature resistant high rejection rolling NF membrane, tolerable temperature 50-90 DEG C, aperture is at 1000um, and molecular cut off, at 300KDalton, isolates the large particulate matter in filtrate and supernatant, wherein large particulate matter repeats to extract as Radix Isatidis residue, and supernatant concentration, spraying are dried Radix Isatidis Chinese medicine is pure.Adopt that high-efficient liquid is pure relative to Radix Isatidis Chinese medicine detects, ensure purity more than 99%.
According to suitable Chinese medicine formula, calculate the content of Radix Isatidis in Chinese medicine formula, the average purification ratio inverse according to Radix Isatidis goes out the pure consumption of required Radix Isatidis Chinese medicine, directly take the consumption that above-mentioned Radix Isatidis Chinese medicine is pure, time saving and energy saving, avoid extraction step, time saving and energy saving.
Embodiment 5
Pure for example with Radix Scutellariae Chinese medicine, first extract Radix Scutellariae Chinese medicine pure, the first step, gets a certain amount of Radix Scutellariae, add the dehydrated alcohol of 10 times amount, decoct 100 minutes at 100 DEG C, distilling under reduced pressure, go out ethanol, obtain mixed liquor, filter mixed liquor obtains filtrate, and the Radix Scutellariae residue of filtration repeats above extraction step; Second step, merge twice filtrate, by high temperature resistant high rejection rolling NF membrane, tolerable temperature 50-90 DEG C, aperture is at 1000um, and molecular cut off, at 300KDalton, isolates the large particulate matter in filtrate and supernatant, wherein large particulate matter repeats to extract as Radix Scutellariae residue, and supernatant concentration, spraying are dried Radix Scutellariae Chinese medicine is pure.Adopt that high-efficient liquid is pure relative to Radix Scutellariae Chinese medicine detects, ensure purity more than 99%.
According to suitable Chinese medicine formula, calculate the content of Radix Scutellariae in Chinese medicine formula, the average purification ratio inverse according to baikal skullcap root goes out the pure consumption of required Radix Scutellariae Chinese medicine, directly take the consumption that above-mentioned Radix Scutellariae Chinese medicine is pure, time saving and energy saving, avoid extraction step, time saving and energy saving.
Embodiment 6
To treat laryngopharynx swelling and pain prescription: bear eastern rattan 5g, Herba Duchesneae Indicae 2g, Herba Menthae 2g, Lindernia angustifolia (Benth.) Wettst. 1g, Herba Diclipterae Chinensis 2g, Herba Oxalidis Corniculatae 2g, Flos Chrysanthemi 1g.
Above medicine extracts the pure method of Chinese medicine according to the present invention, has extracted to bear that eastern rattan Chinese medicine is pure, Herba Duchesneae Indicae Chinese medicine is pure, Herba Menthae Chinese medicine is pure, Lindernia angustifolia (Benth.) Wettst. Chinese medicine is pure, Herba Diclipterae Chinensis Chinese medicine is pure, Herba Oxalidis Corniculatae Chinese medicine is pure, Flos Chrysanthemi Chinese medicine is pure, for subsequent use.By the withdrawal ratio of above Chinese medicine, return that to calculate required Chinese medicine pure, directly take that above Chinese medicine is pure carries out mix homogeneously.
Above-described embodiment, just in order to technical conceive of the present invention and feature are described, its objective is and is one of ordinary skilled in the art can be understood content of the present invention and implement according to this, can not limit the scope of the invention with this.The change of every equivalence made according to the essence of content of the present invention or modification, all should be encompassed in protection scope of the present invention.
Claims (5)
1. the method for a Chinese medicine quantitative criteria, it is characterized in that: Chinese medicine being carried out extraction and isolation, to obtain the Chinese medicine of different Chinese medicine kind pure, adopt efficient Liquid Detection Chinese medicine pure, ensure that Chinese medicine purity is more than 99%, carries out weighing administration so that different types of Chinese medicine is pure as Chinese medicine quantitative criteria.
2. the method for a kind of Chinese medicine quantitative criteria according to claim 1, it is characterized in that: the pure preparation method of described Chinese medicine: the first step, get quantitative Chinese crude drug, add the ethanol of 1-5 times amount, at 55-85 DEG C of alcoholization 30-90 minute, add the water of 1-5 times amount again, at 100 DEG C of water-soluble 30-90 minute, reclaim ethanol, obtain mixed liquor, filter mixed liquor obtains filtrate, and the Chinese medicine residue of filtration repeats above extraction step; Second step, merges twice filtrate, goes out large particulate matter in filtrate and supernatant with microporous filter membrane or Ultra filtration membrane, and wherein large particulate matter repeats to extract as Chinese medicine residue, and supernatant concentration, spraying are dried Chinese medicine is pure.
3. the method for a kind of Chinese medicine quantitative criteria according to claim 1, it is characterized in that: the pure preparation method of described Chinese medicine: the first step, get quantitative Chinese crude drug, add the distilled water of 1-5 times amount, decoct 30-90 minute at 55-85 DEG C, add the distilled water of 1-5 times amount again, at 100 DEG C of water-soluble 30-90 minute, distilling under reduced pressure, obtains the mixed liquor concentrated, filter mixed liquor obtains filtrate, and the Chinese medicine residue of filtration repeats above extraction step; Second step, merges twice filtrate, goes out large particulate matter in filtrate and supernatant with microporous filter membrane or Ultra filtration membrane, and wherein large particulate matter repeats to extract as Chinese medicine residue, and supernatant concentration, spraying are dried Chinese medicine is pure.
4. the method for a kind of Chinese medicine quantitative criteria according to claims 1 to 3, is characterized in that: the pure detection of described Chinese medicine adopts efficient Liquid Detection.
5. the method for a kind of Chinese medicine quantitative criteria according to Claims 2 or 3, is characterized in that: described ultrafilter membrane molecular cut off is 1K-300KDalton.
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| CN201510045300.6A CN104546964A (en) | 2015-01-29 | 2015-01-29 | Traditional Chinese medicine quantification standard method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105106869A (en) * | 2015-09-30 | 2015-12-02 | 青岛友诚高新技术有限公司 | Traditional Chinese medicine composition for treating melanoma and preparation method of traditional Chinese medicine composition |
| CN105169249A (en) * | 2015-09-11 | 2015-12-23 | 青岛友诚高新技术有限公司 | Traditional Chinese medicine composition for treating gout and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101703526A (en) * | 2009-11-12 | 2010-05-12 | 尹享邑 | Preparation method for consubstantial traditional Chinese medicinal materials |
| CN101822697A (en) * | 2009-07-13 | 2010-09-08 | 湖南省中医药研究院 | Preparation method of traditional Chinese medicine ultrafine formula particles |
-
2015
- 2015-01-29 CN CN201510045300.6A patent/CN104546964A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101822697A (en) * | 2009-07-13 | 2010-09-08 | 湖南省中医药研究院 | Preparation method of traditional Chinese medicine ultrafine formula particles |
| CN101703526A (en) * | 2009-11-12 | 2010-05-12 | 尹享邑 | Preparation method for consubstantial traditional Chinese medicinal materials |
Non-Patent Citations (2)
| Title |
|---|
| 张铁军，高文远编著: "《中药配方颗粒的研究与应用》", 30 January 2010, 北京：中国医药科技出版社 * |
| 涂瑶生: "中药配方颗粒的质量控制研究", 《世界科学技术》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105169249A (en) * | 2015-09-11 | 2015-12-23 | 青岛友诚高新技术有限公司 | Traditional Chinese medicine composition for treating gout and preparation method thereof |
| CN105106869A (en) * | 2015-09-30 | 2015-12-02 | 青岛友诚高新技术有限公司 | Traditional Chinese medicine composition for treating melanoma and preparation method of traditional Chinese medicine composition |
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