CN104543333A - Method for processing alcohol-soluble protein of garlic - Google Patents
Method for processing alcohol-soluble protein of garlic Download PDFInfo
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- CN104543333A CN104543333A CN201410825848.8A CN201410825848A CN104543333A CN 104543333 A CN104543333 A CN 104543333A CN 201410825848 A CN201410825848 A CN 201410825848A CN 104543333 A CN104543333 A CN 104543333A
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- garlic
- soluble protein
- alcohol soluble
- protein
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Abstract
The invention discloses a method for processing alcohol-soluble protein of garlic. The method comprises the following specific steps: dissolving garlic protein powder in 90-95% ethanol with the volume being 10-15 times that of the protein powder of the garlic and 90-95% ethanol with the volume being 8-10 times that of the protein powder of the garlic respectively, performing heat preservation extraction twice, combining supernatant liquor, performing vacuum concentration on the supernatant liquor to obtain concentrated liquor, adding water with the volume being 5 times that of the concentrated liquor into the concentrated liquor to settle the alcohol-soluble protein, washing the settled alcohol-soluble protein with purified water twice, and performing vacuum drying to obtain the alcohol-soluble protein of the garlic, wherein a product has the protein content of 94.6-96.5% and the moisture content of 2.5-3.5%. The method expands the application range of the garlic protein; the product has a very high application value and further expands the application range of the garlic protein.
Description
Technical field
The present invention relates to processing of agriculture product field, be specifically related to a kind of processing method of garlic alcohol soluble protein.
Background technology
The research of current scientist both domestic and external to garlic is quite paid attention to, the deep research of system has been carried out from various aspects such as pharmacology, clinical, application, achieve a large amount of scientific achievements, further demonstrating garlic and be not only a kind of good health food, is also a kind of natural medicine with wide application prospect.After particularly effective bioactive ingredients of being rich in of garlic and nutriment composition are extracted application effectively, bring more how useful value to this traditional product of garlic.Garlic has been rich in allicin, protein, amino acid, vitamin, carbohydrate and the nutritional labeling such as mineral matter element and trace element.Existing garlic converted products kind is more, the curing food of various local flavor, still there are allicin sheet, capsule, oral liquid, Garlic beverage etc., but the product of garlic deep processing is still on the low side, in the process that allicin (oil) extracts, a lot of composition is by as useless physical obsolescence, as protein etc., garlic is rich in protein, and be greater than 4% containing protein in fresh garlic, garlic albumen is a kind of glycoprotein, but the covalent complex that glycoprotein is protein and is connected to form with the few chain sugar with branch, at present enough attention are not also obtained to the utilization of garlic protein.The application number that the applicant submitted on November 19th, 2013 is 201310580196.1, name is called the Chinese patent application of " taking garlic as the extraction process that raw material carries out fully utilizing ", disclose and utilize garlic to prepare garlic albumen powder for raw material, and by garlic albumen powder through one, secondary enzymolysis is obtained garlic glycopeptide respectively, amino acid balanced peptide and garlic polypeptide, show the valuable method making full use of garlic protein, but also do not obtain enough application with regard to alcohol dissolubility garlic albumen, garlic alcohol soluble protein has good film forming, improve its plasticity, the sustained release agent of medicine can be widely used as, tablet anti-adhesion agent, make edible freshness-keeping thin coat in the food industry, effectively can extend the holding time of food, there is very high using value, the development and application of in-depth garlic product, the economic worth promoting garlic is the direction of garlic deep processing, the object of the invention is to utilize garlic Protein processing garlic alcohol soluble protein, the application of further expansion garlic albumen and purposes scope.
Summary of the invention
Method of the present invention is that garlic alcohol soluble protein is extracted in solvent processing with ethanol, for realizing the present invention, specifically have employed following technical scheme:
A processing method for garlic alcohol soluble protein, it comprises following processing step:
Step (one) is got garlic albumen powder and is put in extractor, under agitation adding garlic albumen powder weight 10-15 times of concentration is 90-95% ethanolic solution, Steam Heating is passed into through extractor interlayer, be warming up to 45 DEG C, stir insulation lixiviate 2-3h, then after leaving standstill 10-12h, siphon supernatant liquor, it is 90-95% ethanolic solution that its precipitation adds garlic albumen powder weight 8-10 times of concentration again, passes into Steam Heating, be warming up to 45 DEG C through extractor interlayer, stir insulation and extract 1.5-2.5h, then, after leaving standstill 10-12h, siphon supernatant liquor, merges supernatant liquor;
Supernatant liquor after merging is placed in vacuum concentrator by step (two), be 50-70 DEG C in temperature, vacuum is carry out Vacuum Concentration under-0.05 ~-0.1MPa condition, be concentrated into when concentrate weight is former supernatant liquor weight 1/8 and stop concentrating, in concentrate, under agitation add the pure water of concentrate weight 5 times, 10h is left standstill under temperature is 5-10 DEG C of environment, alcohol soluble protein is separated out, then siphon supernatant liquor, lower sediment then adopts centrifuge to set rotating speed as 8000r/min, centrifugal 10min, collecting precipitation washs 2 times with pure water again, each washing all adopts centrifuge to set rotating speed as 8000r/min, centrifugal 10min, acquisition alcohol soluble protein precipitates,
Step (three) by alcohol soluble protein precipitation divides in stainless steel square plate, the thick 2-3cm of the bed of material, put in vacuum desiccator temperature be 50-75 DEG C, vacuum be-0.065 ~-0.1MPa condition under, vacuum drying 12-18h.
Obtaining garlic alcohol soluble protein dry product by above-mentioned processing method, as pulverized further by garlic alcohol soluble protein, crossing 100 mesh sieves, namely obtain garlic alcohol soluble protein powder.
The garlic albumen powder that the present invention relates to, adopt the applicant in the application number that on November 19th, 2013 submits to be 201310580196.1, name is called that Chinese patent application step (seven) method of " taking garlic as the extraction process that raw material carries out fully utilizing " obtains garlic albumen powder, its method is by the rinsing more than 3 times repeatedly of garlic clear water, removing impurity, strip off garlic clove, insert in the hot water of 35-45 DEG C and soak after 2 hours, rub garlic clove, choose except band spot, scar, shrivelled, sick dirty assorted lobe garlic, carry out rinsed clean, inserting containing weight ratio is in the salt boiling water of 6%, within blanching 3-6 minute, carry out Passivation Treatment, obtain passivation garlic clove, garlic clove is added by weight 2-10 pure water doubly, insert after roughly grinding in grinding-wheel grinder, immediately send in colloid mill again and carry out fine grinding 15-20 minute, make uniformly milk-like slurry, insert in stainless steel jacketed pan, interlayer Steam Heating 45-85 DEG C, stir and extract after 30-120 minute, solution and sediment is obtained through centrifugation, sediment separation obtained is through 40-55 DEG C of vacuum drying, pulverize to obtain odourless garlic powder, odourless garlic powder is added by weight 20-40 pure water doubly, fully stir evenly, heating 35-65 DEG C, dissolve with between 1moL/L sodium hydroxide solution adjust ph to 6.8-10, under constantly stirring, soak 2 hours, then leave standstill after 1 hour, use between 0.5moL/L salt acid for adjusting pH value to 3.9-4.9 again, heating boils 1.5 hours, then staticly settle, centrifugation removing supernatant, obtain sediment, again sediment is added by weight the pure water of 10 times amount, stir evenly latter standing 30 minutes, centrifuge dripping moisture obtains garlic albumen, obtain garlic albumen powder after drying.(statement of concrete grammar with reference to specific embodiment 1 and 2 and 7), it be 201310580196.1(publication number is 103598544A that the garlic albumen powder that the application relates to refer to the patent No.) Chinese patent full text as a reference, the document is the statement in order to more clearly describe garlic albumen powder in the present invention, its entire contents is all included in and is carried out reference herein, just looks like be that repeated description is excessively the same in this article for its full text.
In above-mentioned steps (two), preferred concentrated condition is 60-65 DEG C, and vacuum is-0.075
~-0.085MPa.
Vacuum drying described in above-mentioned steps (three), preferred condition is temperature 65-70 DEG C, and vacuum is-0.08
~under-0.09MPa condition, vacuum drying 12-16h.
" % " described in the present invention, than except separately explaining, is all weight percentage.
Beneficial features of the present invention is:
The processing method of a kind of garlic alcohol soluble protein of the present invention, that garlic albumen is processed into protein,alcohol-soluble further, this albumen has good moulding property, edibility garlic alcohol soluble protein preservative film can be made well, effectively can extend the shelf-life of food as encasing food, garlic alcohol soluble protein also can be widely used as sustained release agent, the anti-adhesion agent of medicine, adhesive agent etc., effect of the present invention is the range of application expanding garlic albumen, makes garlic albumen have versatility widely.
Garlic albumen is a kind of glycoprotein, and protein,alcohol-soluble yield is high, and total recovery rate is 68-75%, protein content is 94.6-96.5%, moisture is at 2.5-3.5%, and obtained garlic alcohol soluble protein, not in water-soluble and absolute ethyl alcohol, is dissolved in the ethanol that concentration is 55-85%.
Garlic alcohol soluble protein has good resistance to water, heat resistance, resistance to fat and film forming, and be widely used in the industries such as food, pharmaceuticals, weaving, papermaking, agricultural chemicals, Application and Development garlic alcohol soluble protein has great using value.
The present invention utilizes the deep processing in garlic process, and preparation technology is simple, and easy to operate, consume energy low, low cost of manufacture, can form scale industrial production, and product has good economic benefit.
Detailed description of the invention
Embodiment 1
(1) take garlic albumen powder 10 kilograms and be placed in extractor, under agitation adding 150 kilograms of concentration is the ethanol of 90%, passes into Steam Heating through extractor interlayer, promote temperature to 45 DEG C, stir insulation lixiviate 3h, then leave standstill 12h, siphon supernatant liquor, it is the ethanol of 90% that precipitation is added 100 kilograms of concentration again, through the heating of extractor interlayer, promotes temperature to 45 DEG C, stir insulation and extract 2.5h, then leave standstill 12h, siphon supernatant liquor, merge supernatant liquor, (2) supernatant liquor after merging is placed in vacuum concentrator, it is 65 DEG C in temperature, vacuum carries out Vacuum Concentration under-0.075MPa condition, obtain concentrate 32.5 kilograms, in concentrate, under agitation add the pure water of 162.5 kilograms, being placed in temperature is leave standstill 10h under 5-10 DEG C of environment, alcohol soluble protein is separated out, then siphon supernatant liquor, lower sediment then adopts centrifuge, be 8000r/min at rotating speed, centrifugal 10min, collecting precipitation washs 2 times with pure water again, it is 8000r/min at rotating speed that each washing all adopts centrifugal, centrifugal 10min, acquisition alcohol soluble protein precipitates, (3) divide in stainless steel square plate by the precipitation of alcohol soluble protein, the thick 2-3cm of the bed of material, is placed in vacuum desiccator, temperature be 70 DEG C, vacuum is under-0.09MPa condition, vacuum drying 12h, obtains garlic alcohol soluble protein 6.97 kilograms, and product water content is 2.5%.
Embodiment 2
(1) take garlic albumen powder 10 kilograms and be placed in extractor, under agitation adding 100 kilograms of concentration is the ethanol of 95%, passes into Steam Heating through extractor interlayer, promote temperature to 45 DEG C, stir insulation lixiviate 2h, then leave standstill 10h, siphon supernatant liquor, it is the ethanol of 95% that precipitation is added 80 kilograms of concentration again, through the heating of extractor interlayer, promotes temperature to 45 DEG C, stir insulation and extract 1.5h, then leave standstill 10h, siphon supernatant liquor, merge supernatant liquor, (2) supernatant liquor after merging is placed in vacuum concentrator, it is 60 DEG C in temperature, vacuum carries out Vacuum Concentration under-0.085MPa condition, obtain concentrate 23.75 kilograms, in concentrate, under agitation add the pure water of 118.75 kilograms, being placed in temperature is leave standstill 10h under 5-10 DEG C of environment, alcohol soluble protein is separated out, then siphon supernatant liquor, lower sediment then adopts centrifuge, be 8000r/min at rotating speed, centrifugal 10min, collecting precipitation washs 2 times with pure water again, it is 8000r/min at rotating speed that each washing all adopts centrifugal, centrifugal 10min, acquisition alcohol soluble protein precipitates, (3) divide in stainless steel square plate by the precipitation of alcohol soluble protein, the thick 2-3cm of the bed of material, is placed in vacuum desiccator, temperature be 65 DEG C, vacuum is under-0.08MPa condition, vacuum drying 16h, obtains garlic alcohol soluble protein 7.45 kilograms, and product water content is 3.3%.
Embodiment 3
(1) take garlic albumen powder 10 kilograms and be placed in extractor, under agitation add the ethanol that 120 kg concentration is 92%, pass into Steam Heating through extractor interlayer, promote temperature to 45 DEG C, stir insulation lixiviate 2.5h, then leave standstill 11h, siphon supernatant liquor, it is the ethanol of 92% that precipitation is added 90 kilograms of concentration again, through the heating of extractor interlayer, promotes temperature to 45 DEG C, stir insulation and extract 2h, then leave standstill 11h, siphon supernatant liquor, merge supernatant liquor, (2) supernatant liquor after merging is placed in vacuum concentrator, it is 62 DEG C in temperature, vacuum carries out Vacuum Concentration under-0.08MPa condition, obtain concentrate 27.5 kilograms, in concentrate, under agitation add the pure water of 137.5 kilograms, being placed in temperature is leave standstill 10h under 5-10 DEG C of environment, alcohol soluble protein is separated out, then siphon supernatant liquor, lower sediment then adopts centrifuge, be 8000r/min at rotating speed, centrifugal 10min, collecting precipitation washs 2 times with pure water again, it is 8000r/min at rotating speed that each washing all adopts centrifugal, centrifugal 10min, acquisition alcohol soluble protein precipitates, (3) divide in stainless steel square plate by the precipitation of alcohol soluble protein, the thick 2-3cm of the bed of material, is placed in vacuum desiccator, temperature be 68 DEG C, vacuum is under-0.085MPa condition, vacuum drying 14h, obtains garlic alcohol soluble protein 7.31 kilograms, and product water content is 2.9%.
Claims (6)
1. a processing method for garlic alcohol soluble protein, is characterized in that, comprises the following steps:
Step (one) is got garlic albumen powder and is put in extractor, under agitation adding garlic albumen powder weight 10-15 times of concentration is 90-95% ethanolic solution, Steam Heating is passed into through extractor interlayer, be warming up to 45 DEG C, stir insulation lixiviate 2-3h, then after leaving standstill 10-12h, siphon supernatant liquor, it is 90-95% ethanolic solution that its precipitation adds garlic albumen powder weight 8-10 times of concentration again, passes into Steam Heating, be warming up to 45 DEG C through extractor interlayer, stir insulation and extract 1.5-2.5h, then, after leaving standstill 10-12h, siphon supernatant liquor, merges supernatant liquor;
Supernatant liquor after merging is placed in vacuum concentrator by step (two), be 50-70 DEG C in temperature, vacuum is carry out Vacuum Concentration under-0.05 ~-0.1MPa condition, be concentrated into when concentrate weight is former supernatant liquor weight 1/8 and stop concentrating, in concentrate, under agitation add the pure water of concentrate weight 5 times, 10h is left standstill under temperature is 5-10 DEG C of environment, alcohol soluble protein is separated out, then siphon supernatant liquor, lower sediment then adopts centrifuge to set rotating speed as 8000r/min, centrifugal 10min, collecting precipitation washs 2 times with pure water again, each washing all adopts centrifuge to set rotating speed as 8000r/min, centrifugal 10min, acquisition alcohol soluble protein precipitates,
Step (three) by alcohol soluble protein precipitation divides in stainless steel square plate, the thick 2-3cm of the bed of material, put in vacuum desiccator temperature be 50-75 DEG C, vacuum be-0.065 ~-0.1MPa condition under, vacuum drying 12-18h.
2. the processing method of a kind of garlic alcohol soluble protein as claimed in claim 1, is characterized in that, described step (two) preferably concentrated condition is 60-65 DEG C ,-0.075 ~-0.085MPa.
3. the processing method of a kind of garlic alcohol soluble protein as claimed in claim 1, is characterized in that, the vacuum drying described in described step (three), and preferred condition is 65-70 DEG C, vacuum drying 12-16h under-0.08 ~-0.09MPa condition.
4. the processing method of a kind of garlic alcohol soluble protein according to claim 1 or 3, is characterized in that: carried out further by gained garlic alcohol soluble protein pulverizing, crossing 100 mesh sieves, obtained garlic alcohol soluble protein powder.
5. the garlic alcohol soluble protein product that the processing method described in a claim 1-3 obtains.
6. a garlic alcohol soluble protein, is characterized in that: protein content is 94.6-96.5%, and moisture is 2.5-3.5%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410825848.8A CN104543333A (en) | 2014-12-29 | 2014-12-29 | Method for processing alcohol-soluble protein of garlic |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410825848.8A CN104543333A (en) | 2014-12-29 | 2014-12-29 | Method for processing alcohol-soluble protein of garlic |
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| CN104543333A true CN104543333A (en) | 2015-04-29 |
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|---|---|---|---|
| CN201410825848.8A Withdrawn CN104543333A (en) | 2014-12-29 | 2014-12-29 | Method for processing alcohol-soluble protein of garlic |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1435430A (en) * | 2003-03-03 | 2003-08-13 | 华南理工大学 | Method for extracting prolamine from corn |
| CN1511912A (en) * | 2002-12-31 | 2004-07-14 | 河南莲花集团技术开发公司 | Process for preparing alcohol soluble wheat protein and its use in adhesive |
| CN101434643A (en) * | 2008-12-11 | 2009-05-20 | 广州珠江啤酒股份有限公司 | Method for extracting alcohol soluble protein in malt |
| CN103598544A (en) * | 2013-11-19 | 2014-02-26 | 徐州绿之野生物食品有限公司 | Extraction process for carrying out comprehensive utilization on garlic as raw material |
-
2014
- 2014-12-29 CN CN201410825848.8A patent/CN104543333A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1511912A (en) * | 2002-12-31 | 2004-07-14 | 河南莲花集团技术开发公司 | Process for preparing alcohol soluble wheat protein and its use in adhesive |
| CN1435430A (en) * | 2003-03-03 | 2003-08-13 | 华南理工大学 | Method for extracting prolamine from corn |
| CN101434643A (en) * | 2008-12-11 | 2009-05-20 | 广州珠江啤酒股份有限公司 | Method for extracting alcohol soluble protein in malt |
| CN103598544A (en) * | 2013-11-19 | 2014-02-26 | 徐州绿之野生物食品有限公司 | Extraction process for carrying out comprehensive utilization on garlic as raw material |
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Application publication date: 20150429 |