CN104535705B - Method for synchronously measuring capsaicin and capsorubin in capsicum oleoresin - Google Patents
Method for synchronously measuring capsaicin and capsorubin in capsicum oleoresin Download PDFInfo
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Abstract
The invention provides a method for synchronously measuring capsaicin and capsorubin in a capsicum oleoresin and belongs to the technical field of detection methods of the capsicum oleoresins. According to the method for synchronously measuring the capsaicin and capsorubin in the capsicum oleoresin, an isoabsorptive point graphing method is adopted, correction is performed by virtue of reversed phase high-performance liquid chromatography, the correction coefficient is introduced, and the content of the capsaicin and capsorubin in the capsicum oleoresin can be accurately, rapidly and synchronously measured. Therefore, the defect of dual wavelength spectrophotometry is overcome, the single wavelength direct colorimetric error is reduced, and the method is particularly applicable to the fields of tracking detection and quality analysis of products in industrial production.
Description
Technical field
The invention belongs to the detection method technical field of capsicum oleoresin is and in particular to a kind of Simultaneous Determination capsicum oleoresin
Middle capsaicin and the method for capsorubin.
Background technology
Capsicum oleoresin is a kind of dark red oil liquid extracting from Fructus Capsici, being concentrated to give, and color, smell and taste are all good, are wide
The food additive raw material of general application.The main active of capsicum oleoresin is capsaicin and capsorubin, domestic and international Fructus Capsici
The demand of oleoresin is all very big, since Guizhou Galla Chinensiss company limited first time in 1997 pushes to market it, annual pin
The amount of selling all is being doubled and redoubled, and China's sales volume is more than 100 tons within 2003.For detection capsicum oleoresin product quality and determination
Optimal production process route, the quantitative analysis method setting up reliable capsicum oleoresin is just particularly important.Current not yet shape
Become comparative maturity, simplicity, economy, reliable analysis method, therefore, improving of capsicum oleoresin detection method is urgently to be resolved hurrily.
At present, measure capsorubin and the method for capsaicin mainly has: gb10783-1996, fao UV double colorimetry.This
Outward, sodium nitrite colorimetry, high performance liquid chromatography (hplc), reversed phase high-performance liquid chromatography (rp-hplc) can also be adopted
Deng the content measuring capsorubin and capsaicin.Hplc and rp-hplc instrument cost intensive, and complex operation, are not suitable for
Commercial introduction is applied, and other method once can only measure a kind of content of main component, also needs to sample is carried out before mensure
Preliminary treatment etc..It is additionally, since capsorubin and capsaicin is combined together, both separate extremely difficult, mensure one of which
During composition, another kind of composition often interferes to it.
Additionally, the method measuring binary mixture content has simultaneously: y- reference method, isobestic point graphing method.Work as component
Between absorption spectrum overlap very serious when, y- reference method can be adopted, but capsorubin and capsaicin chemical constitution differ greatly, overlapping
Rate not high it is impossible to use y- reference method.Mixture absorption curve in view of two components is had during intersection point and can be mapped using isobestic point
Method, the present invention overcomes the deficiencies in the prior art, on the basis of existing technology, explores one kind with isobestic point graphing method as base
Plinth, the method simultaneously measuring the content of capsorubin in capsicum oleoresin and capsaicin in conjunction with rp-hplc.
Content of the invention
The technical problem to be solved is, for the deficiencies in the prior art, provides a kind of Simultaneous Determination chilli oil
The method of capsaicin and capsorubin in resin, using isobestic point graphing method, is corrected with the result of rp-hplc, introduces
Correction coefficient, accurately, the content that quick, Simultaneous Determination goes out capsaicin and capsorubin in capsicum oleoresin, both compensate for double wave
The deficiency of long spectrophotography, reduces the error of Single wavelength direct color comparison again, be particularly suitable for product in commercial production with
Track detection and quality analysiss field.
For solving above-mentioned technical problem, the technical solution adopted in the present invention is: in a kind of Simultaneous Determination capsicum oleoresin
Capsaicin and the method for capsorubin, using isobestic point graphing method, are corrected with the result of reversed phase high-performance liquid chromatography,
Introduce correction coefficient, this correction coefficient is reused in new products measure, comprised the following steps:
The first step: the content of capsaicin and capsorubin in capsicum oleoresin sample is measured using isobestic point graphing method,
It is designated as c1And c2；
Second step: rp-hplc determination capsaicin standard substance and capsorubin standard substance, obtain capsaicin
The reversed-phase high-performance liquid chromatography spectrogram of standard substance and capsorubin standard substance；
3rd step: the content of capsaicin and capsorubin in rp-hplc determination capsicum oleoresin sample,
It is designated as c1 ’And c2 ’；
4th step: the correction of capsaicin and capsorubin content in capsicum oleoresin sample, respectively introduce correction coefficient alpha and
β, then α=c1 ’/c1, β=c2 ’/c2；
5th step: calculate the content of capsaicin and capsorubin in capsicum oleoresin, the actual content obtaining capsaicin is α
c1", the actual content of capsorubin is β c2”.
The preparation method of described capsicum oleoresin sample is: weighs Fructus Capsici powder, uses 75% ethanol, solid-to-liquid ratio 1:4(w/v), 40
Extract 4 hours at DEG C, extract twice, extracting solution merges, concentrate, be placed in 40 DEG C of air dry oven, dry to constant weight, obtain
Capsicum oleoresin sample.
The described first step, measures containing of capsaicin and capsorubin in capsicum oleoresin sample using isobestic point graphing method
Amount, specifically includes following steps:
1. accurately weigh capsaicin standard substance, with ethanol as solvent, be configured to standard solution, concentration is 0.03mg/ml；Take
Capsorubin standard substance, with ether as solvent, are configured to standard solution, and concentration is 0.03mg/ml；Then utilize ultraviolet-visible
Spectrophotometer carries out absorbance scanning to capsaicin standard solution and capsorubin standard solution respectively；
2. common absorption point is found out on the absorbance scanning figure of capsorubin standard solution and capsaicin standard solution
λ2, determine λ1；
3. with (0,) and (1,), and (0,) and (1,) make capsorubin standard substance and capsaicin standard
The absorbance of product and relative concentration graph of a relation, a represents capsaicin, and b represents capsorubin；
4. absorbing point λ1And λ2Place measures the absorbance a of capsicum oleoresin sample respectivelyλ1And aλ2, then seek its ratio
Value；
5. calculate the content of capsaicin and capsorubin in capsicum oleoresin sample, be designated as c1And c2.
Beneficial effects of the present invention are as follows:
1. in Simultaneous Determination capsicum oleoresin of the present invention capsaicin and capsorubin method, first use isobestic point method survey
Determine the content of capsaicin and capsorubin, be then corrected by rp-hlpc assay method, introduce correction coefficient, eliminate system
System error, final Accurate Determining goes out the actual content of capsaicin and capsorubin.Experimental cost of the present invention is cheap, easy and simple to handle,
Especially it may use that common cheap mono-wavelength ultraviolet-visible spectrophotometer is simultaneously to two kinds of things co-existing in a system
The content of matter is analyzed, and need not introduce loaded down with trivial details isolation and purification process, so both compensate for dual-wavelength spectrophotometry
The deficiency measuring, greatly reduces the error of Single wavelength direct color comparison again, and dexterously solves high-performance liquid chromatogram determination
The high present situation of testing cost existing for method.
2. present invention additionally comprises the preparation of capsicum oleoresin is so that the present invention can be peppery by the mensure self-control of isobestic point method
Capsorubin in green pepper oleoresin and the content of capsaicin, are then corrected by rp-hlpc method, obtain capsicum oleoresin
Middle actual capsorubin and the content of capsaicin, and then use as standard substance, and standard substance need not be bought again, so big
Amount has saved cost, and method simplicity is it is easy to implement.Even if it is also possible to convenient, accurate so in the case of there is no standard substance
Really the capsorubin in capsicum oleoresin and capsaicin are measured, are particularly suitable for the tracking inspection of product in commercial production
Survey and quality analysiss.
3. high performance liquid chromatography utilizes chromatography means, so that primary standard substance is efficiently separated with impurities, and institute is in this way
It is acknowledged as separating effect in existing various method best, accuracy of analysis also highest method, but the price due to instrument
Costliness, regular job costly, complex operation, need the special technical staff to be tested, limit it industrially
Popularization and application.In the present invention, high performance liquid chromatography only plays the assosting effect of correction measurement, determines correction system in once testing
After number, then this experimental data can be reused in new products measure, without frequently utilizing high performance liquid chromatography
It is measured, greatly reduces testing cost, be particularly suitable for the tracing detection of product and quality analysiss field in commercial production.
The method of capsaicin and capsorubin in Simultaneous Determination capsicum oleoresin of the present invention, surveys initially with isobestic point method
Determine the content of capsaicin and capsorubin in capsicum oleoresin, be then corrected by rp-hplc method, introduce correction system
Number, obtains the actual content of capsorubin in capsicum oleoresin and capsaicin, is skillfully constructed, method is novel, easy, can be fast
Capsorubin in speed, exactly Simultaneous Determination capsicum oleoresin and the content of capsaicin.
Brief description
The present invention is described in further detail below in conjunction with the accompanying drawings.
The reversed-phase high-performance liquid chromatography figure of Fig. 1: capsaicin standard substance；
Fig. 2: the reversed-phase high-performance liquid chromatography figure of self-control capsorubin standard substance；
Fig. 3: capsorubin and the scanning curve figure of capsaicin standard substance, a- capsaicin, b- capsorubin；
The absorbance of Fig. 4: capsorubin and capsaicin and relative concentration graph of a relation；
The reversed-phase high-performance liquid chromatography figure of Fig. 5: capsicum oleoresin.
Specific embodiment
The method of capsaicin and capsorubin in Simultaneous Determination capsicum oleoresin of the present invention, using isobestic point graphing method,
It is corrected with the result of reversed phase high-performance liquid chromatography, introduce correction coefficient, this correction coefficient weight in new products measure
Multiple use, can sequentially include the following steps:
The first step: measure the content of capsaicin and capsorubin in capsicum oleoresin sample using isobestic point graphing method
1) prepare capsicum oleoresin sample
Weigh 25g Fructus Capsici powder, use 75% ethanol, solid-to-liquid ratio 1:4(w/v), extract 4 hours at 40 DEG C, extract twice, extract
Liquid merges, and concentrates, is placed in 40 DEG C of air dry oven, dries to constant weight, obtain capsicum oleoresin sample 5.3337g；
2) utilize isobestic point graphing method to measure the content of capsaicin and capsorubin in capsicum oleoresin sample, be designated as c1
And c2；
1. accurately weigh capsaicin standard substance, with ethanol as solvent, be configured to standard solution, concentration is 0.03mg/ml；Take
Capsorubin standard substance, with ether as solvent, are configured to standard solution, and concentration is 0.03mg/ml；Then utilize ultraviolet-visible
Spectrophotometer carries out absorbance scanning to capsaicin standard solution and capsorubin standard solution respectively；
2. common absorption point is found out on the absorbance scanning figure of capsorubin standard solution and capsaicin standard solution
λ2, determine λ1；
3. with (0,) and (1,), and (0,) and (1,) make capsorubin standard substance and capsaicin standard
The absorbance of product and relative concentration graph of a relation, a represents capsaicin, and b represents capsorubin；
4. absorbing point λ1And λ2Place measures the absorbance a of capsicum oleoresin sample respectivelyλ1And aλ2, then seek its ratio
Value；
5. calculate the content of capsaicin and capsorubin in capsicum oleoresin sample, be designated as c1And c2；
Second step: rp-hplc determination capsaicin standard substance and capsorubin standard substance, obtain capsaicin
The rp-hplc spectrogram of standard substance and capsorubin standard substance；
Weigh capsorubin standard substance 0.2496g, be dissolved in 10.0ml hplc grade methanol, be configured to the peppery of 0.2496g/ml
Green pepper red pigment standard solution；Weigh capsaicin standard substance 0.18mg and be dissolved in the Fructus Capsici that 5ml hplc grade methanol is configured to 0.036mg/ml
Alkali standard solution；Then it is measured using high performance liquid chromatograph；
3rd step: the content of capsaicin and capsorubin in rp-hplc determination capsicum oleoresin sample
1. weigh capsicum oleoresin sample 6.2mg and be dissolved in the solution being configured to 1.24mg/ml in 5.0ml hplc grade methanol；
2. utilize high performance liquid chromatograph to measure capsicum oleoresin sample solution, obtain reversed-phase high-performance liquid chromatography spectrogram,
Calculate the content of capsaicin and capsorubin in capsicum oleoresin sample respectively, be designated as c1 ’And c2 ’；
4th step: the correction of capsaicin and capsorubin content in capsicum oleoresin sample
In conjunction with the measurement result of rp-hplc method, the measurement result with isobestic point graphing method is contrasted, is introduced respectively
Correction coefficient alpha and β, then α=c1 ’/c1, β=c2 ’/c2；
5th step: calculate the content of capsaicin and capsorubin in capsicum oleoresin, the actual content obtaining capsaicin is α
c1", the actual content of capsorubin is β c2”.
In order to be further elucidated with more detail, deepen the understanding of the present invention, rather than limit the present invention, in conjunction with accompanying drawing,
Provide following embodiments.
In the present embodiment, capsorubin is made by oneself for laboratory, and by high performance liquid chromatograph, its purity is surveyed
Fixed, concrete determination step is as follows:
Weigh homemade capsorubin 0.2496g, be dissolved in 10.0ml hplc grade methanol, be configured to the peppery of 0.2496g/ml
Green pepper red pigment standard solution；Weigh capsaicin standard substance 0.18mg and be dissolved in the Fructus Capsici that 5ml hplc grade methanol is configured to 0.036mg/ml
Alkali standard solution；Then it is measured using high performance liquid chromatograph, respectively obtain capsaicin standard substance and self-control capsorubin
Rp-hplc spectrogram, as depicted in figs. 1 and 2.
By contrast Fig. 1 and Fig. 2 it can be seen that laboratory make by oneself Fructus Capsici alkali content in capsorubin ten thousand/several,
Can ignore, so, homemade capsorubin can use as standard substance.
Therefore capsorubin standard substance adopt the homemade capsorubin of laboratory in the present embodiment.
The first step: measure the content of capsaicin and capsorubin in capsicum oleoresin sample using isobestic point graphing method
1) prepare capsicum oleoresin sample
Weigh 25g Fructus Capsici powder, use 75% ethanol, solid-to-liquid ratio 1:4(w/v), extract 4 hours at 40 DEG C, extract twice, extract
Liquid merges, and concentrates, is placed in 40 DEG C of air dry oven, dries to constant weight, obtain capsicum oleoresin sample 5.3337g；
2) isobestic point graphing method measures the content of capsaicin and capsorubin in capsicum oleoresin sample
1. accurately weigh capsaicin standard substance, with ethanol as solvent, be configured to standard solution, concentration is 0.03mg/ml；Take
Capsorubin standard substance, with ether as solvent, are configured to standard solution, and concentration is 0.03mg/ml；Then utilize ultraviolet-visible
Spectrophotometer carries out absorbance scanning to capsaicin standard solution and capsorubin standard solution respectively；
2. common absorption point λ is found out on the absorbance scanning figure of capsorubin standard substance and capsaicin standard solution2,
Determine λ1；；
Can be obtained according to absorption law:
,
Wherein, a- absorbance, a- specific absorbance, c- concentration；
Two formulas are divided by, then；
Cause , when a and b is isobestic point, then have, and set λ2For isobestic point.
As shown in the capsorubin standard substance of Fig. 3 and the scanning curve figure of capsaicin standard substance, capsaicin and capsorubin
There are three intersection points, respectively (242nm, 0.210), (263nm, 0.127), (292nm, 0.107).Consider from sensitivity, choose
Middle intersection point (larger or smaller can cause larger error) 263nm is as λ2.Capsaicin has larger suction at wavelength 280nm
Receive, and the absorbability difference of capsaicin and capsorubin is larger at this wavelength, for reducing both interference, improves measurement
Sensitivity selects 280nm as λ1.
3. absorbance and the relative concentration graph of a relation of capsorubin standard substance and capsaicin standard substance are made
Record capsaicin standard substance and capsorubin standard substance in λ1(280nm) absorbance under is respectively,, carry it into formulaWithIn, obtain,；
Also, λ2For isobestic point, so,
If,, wherein, x, y represent relative concentration；
Make x=0, obtain==2.6096；
Make x=1 again, obtain=0.9456；
Then capsorubin standard is done with (0,0.9456) and (1,2.6096) and (0,2.6096) and (1,0.9456)
The absorbance of product and capsaicin standard substance and the graph of a relation of relative concentration, as shown in Figure 4.
4. absorbing point λ respectively1And λ2The lower absorbance measuring capsicum oleoresin sample, and calculate capsicum oleoresin sample
The content of capsaicin and capsorubin in product
In wavelength X2=263nm and λ1At=280nm, test the absorbance of capsicum oleoresin sample respectively, refering to table 1, and count
Calculate/.
The table 1 capsicum oleoresin sample absorbance in wavelength 263nm and 280nm respectively
As table 1, the absorbance of capsicum oleoresin sample is in λ1(280nm)、λ2(263nm) place records the absorbance of sample and divides
It is not: aλ1=1.227, aλ2=0.993, try to achieve ratio=1.236, and find out the numerical value of corresponding x and y in the diagram, then root
According to, try to achieve ()；
Substitution formula:With；
Try to achieveWith.
Then, then willWithSubstitution below equation:
Content c of capsaicin in capsicum oleoresin sample1=ca×v/m
Content c of capsorubin in capsicum oleoresin sample2=cb×v/m
In formula: caContent mg/ml of capsaicin in-capsicum oleoresin sample；V- liquor capacity ml；The chilli oil that m- measures
Quality g of resin sample.
Result of calculation is: in capsicum oleoresin sample, the content of capsaicin and capsorubin is respectively c1=7.28mg/g and c2
=17.29mg/g.
Second step: rp-hplc determination capsaicin standard substance and capsorubin standard substance, obtain capsaicin
The rp-hplc spectrogram of standard substance and capsorubin standard substance,
This step front already described, repeats no more, and the rp-hplc spectrogram of capsaicin standard substance and capsorubin standard substance is as schemed
Shown in 1 and Fig. 2.
3rd step: the content of capsaicin and capsorubin in rp-hplc determination capsicum oleoresin sample
1. weigh capsicum oleoresin sample 6.2mg and be dissolved in the solution being configured to 1.24mg/ml in 5.0ml hplc grade methanol；
2. utilize high performance liquid chromatograph to measure capsicum oleoresin sample solution, obtain reversed-phase high-performance liquid chromatography spectrogram,
As shown in figure 5, the content calculating capsaicin and capsorubin in capsicum oleoresin sample respectively with reference to Fig. 1 and Fig. 2, it is designated as c1 ’With
c2 ’；
From fig. 1, it can be seen that the peak of retention time about 3.47min should be the dissolvent residual of high polarity or other impurity formed
Peak.Capsaicin has larger absorption in 280nm, and its retention time is 15.90min, and area integral is 663656, accounts for the gross area
63.26%；And last larger peak is dihydrocapsaicin, its retention time is 23.32min, and area integral is 246457, accounts for
The 23.49% of the gross area.Can be seen that, with reference to Fig. 1, the peak that retention time is 16.00min by Fig. 5 is capsaicin, its area integral
For 500696；And the peak that another retention time is 22.26 is dihydrocapsaicin, its area integral is 246958.
In conjunction with the concentration of capsaicin in the area integral of capsaicin in Fig. 1 and Fig. 5, and capsaicin standard substance, can calculate
Go out content c of capsaicin in capsicum oleoresin1 ’For 13.85mg/g, the content of dihydrocapsaicin is 6.83mg/g.
Because homemade capsorubin is through purity testing, its purity is approximately 100%, does not enter one to it in the present embodiment
Step is corrected using rp-hplc；In addition it is also possible to rp-hplc mensure is carried out to it, it is designated as c2 ’.
4th step: the correction of capsaicin and capsorubin content in capsicum oleoresin sample
To correct the systematic error of isobestic point method using high performance liquid chromatography, in order to evaluate the precision of the method.
The content that reversed phase high-performance liquid chromatography records capsaicin in capsicum oleoresin is 13.85mg/g, and isobestic point method mensure is peppery
The result of green pepper alkali is 7.28mg/g, 7.34mg/g and 7.22mg/g, and its meansigma methods is 7.28mg/g, and linearly preferable.In order to adopt
With more practical isobestic point graphing method, solve the problems, such as its more low systematic error, introduce a correction coefficient, reference
The result of rp-hplc, this correction coefficient should be α=c1 ’/c1=13.85/7.28=1.90.
For capsorubin, it would however also be possible to employ said method is corrected, introduce correction coefficient β, then β=c2 ’/c2；
And in the present embodiment, because capsorubin is homemade, and verify its purity close to 100% through high performance liquid chromatography, without school
Just, or be interpreted as that correction coefficient is β=1.
5th step: calculate the content of capsaicin and capsorubin in capsicum oleoresin
Actual Fructus Capsici alkali content should be result c of isobestic point method calculating1It is multiplied by 1.90 times again, i.e. the reality of capsaicin
Content is c1 ’=αc1=1.90×7.28mg/g=13.85mg/g；
The actual content of capsorubin is c2 ’=βc2=1×17.29mg/g=17.29mg/g.
Using the isobestic point graphing method of the present invention, then with rp-hplc method correction after, then need not repurchase standard substance.This
Bright experimental cost is cheap, easy and simple to handle, and especially it can use common cheap mono-wavelength ultraviolet-visible spectrophotometer simultaneously
The content of two kinds of materials co-existing in a system is analyzed, and loaded down with trivial details isolation and purification need not be introduced.Both compensate for
The deficiency of Dual-Wavelength Spectrophotometric Determination, reduces the error of Single wavelength direct color comparison again, is particularly suitable in commercial production
The tracing detection of product and quality analysiss.Isobestic point graphing method corrects through rp-hplc, can be used as a kind of very novel, letter
Just, the method for quick synchronous detecting capsaicin and capsorubin.
In sum, in a kind of Simultaneous Determination of present invention capsicum oleoresin, the method for capsaicin and capsorubin is addressed
Every claim and technical support clearly, any modification and change that all technical supports according to the present invention are substantially made
Still fall within the range of the technology of the present invention support.
Claims (2)
1. in a kind of Simultaneous Determination capsicum oleoresin capsaicin and capsorubin method, using isobestic point graphing method, with anti-
The result of phase high performance liquid chromatography is corrected, and introduces correction coefficient, and this correction coefficient repeats to make in new products measure
With it is characterised in that comprising the following steps:
The first step: measure the content of capsaicin and capsorubin in capsicum oleoresin sample using isobestic point graphing method, specifically
Comprise the following steps:
1. accurately weigh capsaicin standard substance, with ethanol as solvent, be configured to standard solution, concentration is 0.03mg/ml；Take Fructus Capsici
Red pigment standard substance, with ether as solvent, are configured to standard solution, and concentration is 0.03mg/ml；Then utilize UV-vis spectroscopy
Photometer carries out absorbance scanning to capsaicin standard solution and capsorubin standard solution respectively；
2. common absorption point λ is found out on the absorbance scanning figure of capsorubin standard solution and capsaicin standard solution2, really
Determine λ1；
3. with (0,) and (1,), and (0,) and (1,) make capsorubin standard substance and capsaicin standard
The absorbance of product and relative concentration graph of a relation, a represents capsaicin, and b represents capsorubin；
4. absorbing point λ1And λ2Place measures the absorbance a of capsicum oleoresin sample respectivelyλ1And aλ2, then seek its ratio；
5. calculate the content of capsaicin and capsorubin in capsicum oleoresin sample, be designated as c1And c2；
Second step: rp-hplc determination capsaicin standard substance and capsorubin standard substance, obtain capsaicin standard
The reversed-phase high-performance liquid chromatography spectrogram of product and capsorubin standard substance；
3rd step: the content of capsaicin and capsorubin in rp-hplc determination capsicum oleoresin sample, it is designated as
c1 ’And c2 ’；
4th step: the correction of capsaicin and capsorubin content in capsicum oleoresin sample, introduce correction coefficient alpha and β respectively, then
α=c1 ’/c1, β=c2 ’/c2；
5th step: calculate the content of capsaicin and capsorubin in capsicum oleoresin, the actual content obtaining capsaicin is α c1 ’’,
The actual content of capsorubin is β c2 ’’.
2. in Simultaneous Determination capsicum oleoresin as claimed in claim 1 capsaicin and capsorubin method it is characterised in that
The preparation method of described capsicum oleoresin sample is: weighs Fructus Capsici powder, uses 75% ethanol, solid-to-liquid ratio 1:4(w/v), extract at 40 DEG C
4 hours, extract twice, extracting solution merges, concentrate, be placed in 40 DEG C of air dry oven, dry to constant weight, obtain chilli oil tree
Fat sample.
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| CN107656000B (en) * | 2017-10-26 | 2021-04-09 | 晨光生物科技集团股份有限公司 | Method for analyzing and identifying capsaicin compounds in capsicum oleoresin |
| CN108181397A (en) * | 2017-12-29 | 2018-06-19 | 浙江农林大学 | Hangzhou chili capsaicine concentration extraction measuring method |
| CN108414666B (en) * | 2018-04-23 | 2020-05-05 | 河南科高中标检测技术有限公司 | Method for measuring gingerol content in ginger medicinal material volatile oil extract |
| CN108414665B (en) * | 2018-04-23 | 2020-05-05 | 河南科高中标检测技术有限公司 | Method for measuring gingerol content in ginger medicinal material and preparation thereof |
| CN112945879A (en) * | 2021-02-07 | 2021-06-11 | 四川省农业科学院园艺研究所 | Synergistic detection method for color value of capsaicin and capsorubin |
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| CN1266226C (en) * | 2004-08-26 | 2006-07-26 | 上海交通大学 | Process for separating capsorubin and capsaicine from hot pepper |
| CN101082609A (en) * | 2006-05-30 | 2007-12-05 | 田丽萍 | Method for detecting natural colouring matter pimiento red pigment in food product |
| CN102060728A (en) * | 2010-12-20 | 2011-05-18 | 重庆市农业科学院 | Method for extracting capsorubin and capsaicin from chillies |
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2014
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