CN102944627B - Method for detecting content of four pigments in tobacco shreds for cigarettes - Google Patents
Method for detecting content of four pigments in tobacco shreds for cigarettes Download PDFInfo
- Publication number
- CN102944627B CN102944627B CN201210492584XA CN201210492584A CN102944627B CN 102944627 B CN102944627 B CN 102944627B CN 201210492584X A CN201210492584X A CN 201210492584XA CN 201210492584 A CN201210492584 A CN 201210492584A CN 102944627 B CN102944627 B CN 102944627B
- Authority
- CN
- China
- Prior art keywords
- amaranth
- solution
- standard
- kinds
- yellow
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Sampling And Sample Adjustment (AREA)
- Cigarettes, Filters, And Manufacturing Of Filters (AREA)
Abstract
A method for detecting the content of four pigments (lemon yellow, amaranth, carmine and sunset yellow) in tobacco shreds for cigarettes comprises the steps of preparing a sample solution, performing liquid chromatogram analysis, calculating detected results and the like. After optimized, the method has the advantages of being short in detection time, simple and convenient to operate, high in sensitivity and recovery rate, good in repeatability and the like. The chromatographic condition of the method enables the lemon yellow, the amaranth, the carmine and the sunset yellow to be separated well, the method has good correlation, a detecting range is limited from 0.02mug to 0.03mug, the average recovery rates are respectively 102.47%, 92.48%, 89.94% and 96.66%, and average relative standard deviations of sample testing results are respectively 3.18%, 2.63%, 2.74% and 3.02%, so that the method is high in recovery rate and good in repeatability. The method for detecting the content of four pigments in tobacco shreds for cigarettes provides a scientific basis for quality surveillance of the cigarettes.
Description
Technical field
The invention belongs to the physical and chemical inspection technical field of cigarette with auxiliary material, be specifically related to the assay method of cigarette with four kinds of pigments (lemon yellow, amaranth, famille rose and sunset yellow) content in cigarette shreds.
Background technology
The synthetic food colors such as amaranth, famille rose, lemon yellow, sunset yellow are synthetic through a series of more complicated technological processs such as oversulfonate, nitrated, halogenation, azo take chemical products such as benzene, toluene, naphthalenes as raw material, therefore have certain toxicity, but because of its cost low, bright in colour, strong coloring force, be widely used.But a large amount of research reports is pointed out, nearly all synthetic dyestuff all can not provide nutriment to human body, some synthetic dyestuff even can be detrimental to health, and causes fertility decline, monster etc., and some synthetic dyestuff may convert carcinogen in human body.The harm of synthetic dyestuff comprises general toxicity, causes rushing down property, causes prominent property (gene mutation) and carcinogenesis.Result of study shows, the food that children's often takes man-made additive easily causes some harm.
Countries in the world especially western developed country have not only been done a large amount of investigation and research at pigment aspect Health Impact, and in the management of food coloring, the use of synthetic dyestuff, strict regulation being arranged all, multiple synthetic dyestuff has been prohibited or the use of strictly limiting the quantity of.
Most countries strictly limits some pigment, the particularly use of azo pigment in food.Through effort for many years, edible synthesized coloring matter kind that now can legal use greatly reduces.While in countries in the world, using synthetic dyestuff maximum, kind nearly more than 100 is planted, and the secondary colour that once approval of Japan is used have 27 kinds, has now banned use of wherein 16 kinds.The secondary colour that the U.S. allows to use nineteen sixty have 35 kinds, existing only remaining 7 kinds.Sweden, Finland, Norway, India, Denmark, France etc. ban use of the azo pigment already, wherein some countries such as Norway also total bans use any chemosynthesis pigments.Also have some countries to forbid adding synthetic dyestuff in the food such as meat, fish and processed goods thereof, fruit and goods thereof, flavouring, baby food, cake in addition.
Also there is strict restriction in China in food, adding synthetic dyestuff: flavouring, fruit and goods thereof, newborn class and dairy products, baby food, biscuit, the cakes such as every meat and processed goods thereof, fish and processed goods thereof, vinegar, soy sauce, fermented bean curd all can not use synthetic food color.Only having carbonated drink, cold drink food, candy, assembled alcoholic drinks and fruit juice to reveal can use on a small quantity, generally must not surpass 1/10000.
The assay method of synthetic food color is a lot, as high performance liquid chromatography, capillary electrophoresis, oscilloscopic polarography, spectrophotometric method, thin-layered chromatography etc., wherein high performance liquid chromatography is the most frequently used assay method, it is high that application HPLC method is measured synthetic hybrid pigment accuracy, method is sensitive, favorable reproducibility, can measure several hybrid pigments simultaneously.
Summary of the invention
purpose of the present invention just is being based on above-mentioned prior art situation, be intended to overcome the prior art defect, four kinds of pigment (lemon yellows in a kind of cigarette shreds are provided, amaranth, famille rose and sunset yellow) assay method of content, the present invention has investigated four kinds of pigment (lemon yellows in cigarette shreds, amaranth, famille rose and sunset yellow) extraction conditions, optimized the condition of chromatographic resolution, for easy, measure fast and effectively four kinds of pigment (lemon yellows in cigarette shreds, amaranth, famille rose and sunset yellow) residual content provides technical support, the foundation of science is provided for the quality supervision of cigarette.
The objective of the invention is to be achieved through the following technical solutions:
The assay method of four kinds of pigment lemon yellows, amaranth, famille rose and sunset yellow content in cigarette shreds comprises the following steps:
(1) preparation of standard solution: preparation has the hybrid standard working solution (namely four kinds of materials mix and are mixed with the standard solution of series concentration) of lemon yellow, amaranth, famille rose and the sunset yellow of concentration gradient respectively;
The compound method of described standard solution is as follows: take each 25mg(of four kinds of pigments (lemon yellow, amaranth, famille rose and sunset yellow) and be accurate to 0.1mg), with the high purity water solution transfer, to the 50mL volumetric flask, be settled to scale and shake up, as the one-level mother liquor; Get in one-level mother liquor 1mL to 50mL volumetric flask, with ultrapure water, be settled to scale, as the secondary mother liquor; Accurately pipette respectively 10 μ L, 50 μ L, 100 μ L, 200 μ L, 500 μ L, the secondary mother liquor of 1000 μ L, to the 10mL volumetric flask, is settled to scale with ultrapure water, obtains the series standard working solution.
(2) inner mark solution preparation: take the light blue standard items, with high purity water, dissolve, obtaining concentration is the light blue standard solution of 10 μ g/mL, as internal standard compound matter;
The concrete compound method of inner mark solution is: take the light blue standard items of 10mg, be accurate to 0.1mg, in the volumetric flask of 1000mL, with high purity water, dissolve and constant volume, obtaining concentration is the light blue standard solution of 10 μ g/mL, as internal standard compound matter.
(3) preparation of sample solution: use pure water as extractant, then cross C-18 Solid-Phase Extraction column purification sample solution;
The preparation method of described sample solution is: take the pipe tobacco of 2 cigarette in 50mL tool plug conical flask, accurately add ultrapure water 30mL, interior mark liquid 100 μ L, on oscillator in 150r/min oscillation extraction 30min; Filter out pipe tobacco, get 5mL filtrate and cross the C-18 solid-phase extraction column, collect filtrate and eluent, as the sample solution of stratographic analysis.
(4) liquid-phase chromatographic analysis: utilize liquid chromatograph respectively standard solution and sample solution to be detected to analysis;
Described chromatographiccondition is chromatographic column adopting Agela Venusil MP C18 liquid-phase chromatographic column, and chromatogram column temperature is 30 ℃, and sample size is 10 μ L, and flow velocity is 1.0 mL/min; Diode array detector detects wavelength: lemon yellow is 430 nm, and amaranth is 520 nm, and famille rose is 510 nm, sunset yellow 482 nm; Mobile phase: A is the 0.02mol/L ammonium acetate, and B is acetonitrile, adopts the gradient elution program, and namely initial A is 90%, when B is 10%, 10min A be 30%, B while being 70%, 13min A be 90%, when B was 10%, 15min, A was that 50%, B is 50%, and total elution time is 15min.
(5) calculating of four kinds of pigment lemon yellows, amaranth, famille rose and sunset yellow content in cigarette shreds.
Circular is as follows: at first standard substance is mixed with to certain density standard solution, and the interior mark liquid (the light blue standard solution of 10 μ g/mL) that adds 50 μ L mixes rear sample introduction analysis, ratio with the peak area of the peak area of internal standard compound and object carries out regretional analysis to its respective standard solution concentration, obtains standard working curve; Then the object that sample solution is detected and detected internal standard compound peak area ratio, the substitution standard working curve; Namely obtain the content of four kinds of pigments (lemon yellow, amaranth, famille rose and sunset yellow) in sample.
Detection method of the present invention is optimized disposal route and the chromatographic condition of sample, has reached following effect:
(1) detection time is short: adopting the present invention to measure four kinds of pigment (lemon yellow, amaranth, famille rose and sunset yellow) content cycles in cigarette shreds only needs 15 minutes;
(2) the present invention have advantages of easy and simple to handle, highly sensitive, the recovery is high and reproducible: the chromatographic condition of the inventive method makes four kinds of pigments (lemon yellow, amaranth, famille rose and sunset yellow) chromatographic peak in cigarette shreds separate better with the impurity chromatographic peak, and has correlativity preferably, detectability is between 0.02～0.03 μ g/g, average recovery rate is respectively 102.47%, 92.48%, 89.94% and 96.66%, and the average relative standard deviation of sample test result is respectively 3.18%, 2.63%, 2.74% and 3.02%.
The accompanying drawing explanation
Fig. 1 is the process flow diagram of assay method of the present invention;
Fig. 2 is typical curve linear equation and the linear regression coeffficient coordinate diagram of lemon yellow in cigarette shreds;
Fig. 3 is typical curve linear equation and the linear regression coeffficient coordinate diagram of amaranth in cigarette shreds;
Fig. 4 is typical curve linear equation and linear regression coeffficient coordinate diagram carmine in cigarette shreds;
Fig. 5 is typical curve linear equation and the linear regression coeffficient coordinate diagram of sunset yellow in cigarette shreds;
Fig. 6 is standard solution 430nm place lemon yellow spectrogram;
Fig. 7 is standard solution 520nm place amaranth chromatogram;
Fig. 8 is standard solution 510nm place kermes spectrogram;
Fig. 9 is standard solution 482nm place sunset yellow chromatogram;
Figure 10 is standard solution 630nm place internal standard compound chromatogram;
Figure 11 is sample solution 430nm place lemon yellow spectrogram;
Figure 12 is sample solution 520nm place amaranth chromatogram;
Figure 13 is sample solution 510nm place kermes spectrogram;
Figure 14 is sample solution 482nm place sunset yellow chromatogram;
Figure 15 is sample solution 630nm place internal standard compound chromatogram;
Embodiment
The present invention is described further below in conjunction with embodiment (accompanying drawing):
The assay method following (process flow diagram of described detection method as shown in Figure 1) of the present embodiment to four kinds of pigments (lemon yellow, amaranth, famille rose and sunset yellow) content in cigarette shreds
(1) preparation of standard solution: take each 25mg(of four kinds of pigments (lemon yellow, amaranth, famille rose and sunset yellow) and be accurate to 0.1mg), with the ultrapure water solution transfer, to the 50mL volumetric flask, be settled to scale and shake up, as the one-level mother liquor; Get in one-level mother liquor 1mL to 50mL volumetric flask, with ultrapure water, be settled to scale, as the secondary mother liquor; Accurately pipette respectively 10 μ L, 50 μ L, 100 μ L, 200 μ L, 500 μ L, the secondary mother liquor of 1000 μ L, to the 10mL volumetric flask, is settled to scale with ultrapure water, obtains the series standard working solution, is stored in 2 ℃-8 ℃, the term of validity 1 month.
Take the light blue standard items of 10mg left and right, be accurate to 0.1mg, in the volumetric flask of 100mL, with ultrapure water, dissolve and constant volume, obtaining concentration is the light blue standard solution liquid of 10 μ g/mL, as internal standard compound matter.Be stored in 2 ℃-8 ℃, the term of validity 1 month.
(2) preparation of sample solution: take the pipe tobacco of 2 cigarette in 50mL tool plug conical flask, accurately add ultrapure water 30mL, interior mark liquid 100 μ L, on oscillator in 150r/min oscillation extraction 30min; Filter out pipe tobacco, get 5mL filtrate and cross the C18 solid-phase extraction column, collect filtrate and eluent, on liquid chromatograph, analyze.
(3) stratographic analysis: described chromatographiccondition is chromatographic column adopting Agela Venusil MP C18 liquid-phase chromatographic column, and chromatogram column temperature is 30 ℃, and sample size is 10 μ L, and flow velocity is 1.0 mL/min; Diode array detector detects wavelength: lemon yellow is 430 nm, and amaranth is 520 nm, and famille rose is 510 nm, sunset yellow 482 nm; Mobile phase: A is the 0.02mol/L ammonium acetate, and B is acetonitrile, adopts the gradient elution program, and namely initial A is 90%, when B is 10%, 10min A be 30%, B while being 70%, 13min A be 90%, when B was 10%, 15min, A was that 50%, B is 50%, and total elution time is 15min.The stratographic analysis result of standard solution is as shown in Fig. 6-10; The stratographic analysis result of sample solution is as shown in Figure 11-15;
(4) calculating of four kinds of pigments (lemon yellow, amaranth, famille rose and sunset yellow) content in cigarette shreds: at first standard substance is mixed with to certain density standard solution, and add certain density interior mark liquid, sample introduction analysis after mixing, ratio with the peak area of the peak area of internal standard compound and object carries out regretional analysis to its respective standard solution concentration, obtains standard working curve (referring to Fig. 2-5); Then the object that sample solution is detected and detected internal standard compound peak area ratio, the substitution standard working curve; Namely obtain the content of four kinds of pigments (lemon yellow, amaranth, famille rose and sunset yellow) in sample.
Then the chromatographic peak area that detects object that sample is recorded, the substitution typical curve, namely obtain four kinds of pigments (lemon yellow, amaranth, famille rose and the sunset yellow) quality in sample, calculate thus the content of sunset yellow in cigarette, lemon yellow, famille rose and amaranth, computing formula is as follows:
In formula:
The content of lemon yellow, amaranth, famille rose and sunset yellow in m-every gram pipe tobacco, unit are the every gram of microgram (μ g/g);
The quality of lemon yellow, amaranth, famille rose and sunset yellow in A-sample (μ g);
N-the take quality (g) of sample.
As can be known by table 1 and accompanying drawing 4, the chromatographic condition that adopts makes four kinds of pigments (lemon yellow, amaranth, famille rose and sunset yellow) chromatographic peak separate better with the impurity chromatographic peak, and has correlativity preferably, and detectability is between 0.02-0.03 μ g/g.The content of the lemon yellow of the present embodiment sample, amaranth, famille rose and sunset yellow is respectively 22.36 μ g/g, 17.26 μ g/g, 18.65 μ g/g and 19.47 μ g/g.
The present embodiment is as follows to the detection method of the repeatability of the inventive method and recovery of standard addition
Adopt the test of sample recovery of standard addition, in sample, add respectively the standard solution of a low middle Senior Three variable concentrations to carry out the recovery of standard addition test, each sample is measured respectively 6 times, the condition of stratographic analysis is with embodiment 1, according to the relative standard deviation of measured value after the recovery of standard addition of lemon yellow, amaranth, famille rose and sunset yellow in Analysis result calculation this method pipe tobacco and mark-on, result is as shown in table 2:
As can be seen from Table 2, on 3 mark-on levels, the average recovery rate that utilizes the method to detect four kinds of pigments (lemon yellow, amaranth, famille rose and sunset yellow) in cigarette shreds is respectively 102.47%, 92.48%, 89.94% and 96.66%, the average relative standard deviation of sample test result is respectively 3.18%, 2.63%, 2.74% and 3.02%, the recovery that this law is described is higher, and repeatability better.
Claims (5)
1. the assay method of four kinds of pigment lemon yellows, amaranth, famille rose and sunset yellow content in a cigarette shreds is characterized in that: comprise the following steps:
(1) preparation of standard solution: preparation has the hybrid standard working solution of lemon yellow, amaranth, famille rose and the sunset yellow of concentration gradient respectively;
(2) inner mark solution preparation: take the light blue standard items, with high purity water, dissolve, obtaining concentration is the light blue standard solution of 10 μ g/mL, as internal standard compound matter;
(3) preparation of sample solution: use pure water as extractant, then cross C-18 Solid-Phase Extraction column purification sample solution;
(4) liquid-phase chromatographic analysis: utilize liquid chromatograph respectively standard solution and sample solution to be detected to analysis; Described chromatographiccondition is chromatographic column adopting C18 liquid-phase chromatographic column, and chromatogram column temperature is 30 ℃, and sample size is 10 μ L, and flow velocity is 1.0 mL/min; Diode array detector detects wavelength: lemon yellow is 430 nm, and amaranth is 520 nm, and famille rose is 510 nm, sunset yellow 482 nm; Mobile phase: A is the 0.02mol/L ammonium acetate, and B is acetonitrile, adopts the gradient elution program, and namely initial A is 90%, when B is 10%, 10min A be 30%, B while being 70%, 13min A be 90%, when B was 10%, 15min, A was that 50%, B is 50%, and total elution time is 15min;
(5) calculating of four kinds of pigment lemon yellows, amaranth, famille rose and sunset yellow content in cigarette shreds.
2. the assay method of four kinds of pigment lemon yellows, amaranth, famille rose and sunset yellow content in cigarette shreds according to claim 1, it is characterized in that: the compound method of described standard solution is as follows: take four kinds of pigments: each 25mg of lemon yellow, amaranth, famille rose and sunset yellow, be accurate to 0.1mg, with the high purity water solution transfer to the 50mL volumetric flask, be settled to scale and shake up, as the one-level mother liquor; Get in one-level mother liquor 1mL to 50mL volumetric flask, with ultrapure water, be settled to scale, as the secondary mother liquor; Accurately pipette respectively 10 μ L, 50 μ L, 100 μ L, 200 μ L, 500 μ L, the secondary mother liquor of 1000 μ L, to the 10mL volumetric flask, is settled to scale with ultrapure water, obtains the series standard working solution.
3. the assay method of four kinds of pigment lemon yellows, amaranth, famille rose and sunset yellow content in cigarette shreds according to claim 1, it is characterized in that: the concrete compound method of inner mark solution is: the light blue standard items that take 10mg, be accurate to 0.1mg, in the volumetric flask of 1000mL, with high purity water, dissolve and constant volume, obtaining concentration is the light blue standard solution of 10 μ g/mL, as internal standard compound matter.
4. the assay method of four kinds of pigment lemon yellows, amaranth, famille rose and sunset yellow content in cigarette shreds according to claim 1, it is characterized in that: the preparation method of described sample solution is: take the pipe tobacco of 2 cigarette in 50mL tool plug conical flask, accurately add ultrapure water 30mL, interior mark liquid 100 μ L, on oscillator in 150r/min oscillation extraction 30min; Filter out pipe tobacco, get 5mL filtrate and cross the C-18 solid-phase extraction column, collect filtrate and eluent, as the sample solution of stratographic analysis.
5. four kinds of pigment lemon yellows in cigarette shreds according to claim 1, amaranth, the assay method of famille rose and sunset yellow content, it is characterized in that: four kinds of pigment sunset yellows in described cigarette shreds, lemon yellow, computing method carmine and amaranth content are as follows: at first standard substance is mixed with to certain density standard solution, and to add concentration be the inner mark solution 50 μ L of 10 μ g/mL, sample introduction analysis after mixing, ratio with the peak area of the peak area of internal standard compound and object carries out regretional analysis to its respective standard solution concentration, obtain standard working curve, then the object that sample solution is detected and detected internal standard compound peak area ratio, the substitution standard working curve, namely obtain the content of four kinds of pigment lemon yellows, amaranth, famille rose and sunset yellows in sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210492584XA CN102944627B (en) | 2012-11-28 | 2012-11-28 | Method for detecting content of four pigments in tobacco shreds for cigarettes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210492584XA CN102944627B (en) | 2012-11-28 | 2012-11-28 | Method for detecting content of four pigments in tobacco shreds for cigarettes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102944627A CN102944627A (en) | 2013-02-27 |
| CN102944627B true CN102944627B (en) | 2013-11-20 |
Family
ID=47727589
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210492584XA Active CN102944627B (en) | 2012-11-28 | 2012-11-28 | Method for detecting content of four pigments in tobacco shreds for cigarettes |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102944627B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105403630A (en) * | 2015-10-27 | 2016-03-16 | 云南瑞升烟草技术（集团）有限公司 | Method for detecting concentration of nine compound coloring agents in auxiliary materials for reconstituted tobacco |
| CN105699465B (en) * | 2016-03-09 | 2018-08-17 | 福州大学 | A kind of method that pressurization capillary electric chromatogram multi-mode continuously detaches synthetic dyestuff |
| CN106093216B (en) * | 2016-05-27 | 2018-11-06 | 国家烟草质量监督检验中心 | The high performance liquid chromatography test method of 11 kinds of colorants in a kind of tobacco leaf |
-
2012
- 2012-11-28 CN CN201210492584XA patent/CN102944627B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN102944627A (en) | 2013-02-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Minioti et al. | Determination of 13 synthetic food colorants in water-soluble foods by reversed-phase high-performance liquid chromatography coupled with diode-array detector | |
| CN104730186B (en) | Precolumn derivatization-UPLC(ultra performance liquid chromatography)-ESI(electronic spray ion)+-MS/MS (mass spectrometry) detection method of glyphosate and glufosinate-ammonium pesticide residue in tea | |
| Liao et al. | Applicability of accelerated solvent extraction for synthetic colorants analysis in meat products with ultrahigh performance liquid chromatography–photodiode array detection | |
| CN104251893A (en) | Method for determining colorant in cigarette package material | |
| Francis et al. | A rapid antioxidant assay based on acidic potassium permanganate chemiluminescence | |
| CN106990183A (en) | The method for chromatographic determination of synthetic coloring matter in a kind of food | |
| CN102944627B (en) | Method for detecting content of four pigments in tobacco shreds for cigarettes | |
| CN107091892A (en) | The method that the content of synthetic coloring matter in food is tested using high performance liquid chromatography | |
| CN104535705B (en) | Method for synchronously measuring capsaicin and capsorubin in capsicum oleoresin | |
| CN101876638B (en) | Simultaneous and rapid detection method for benzo(a)pyrene, benzo(k)fluoranthene and anthracene in tea | |
| CN109655570A (en) | The measuring method of prothioconazoles residual quantity in a kind of food | |
| CN103604759A (en) | Rapid screening kit for synthetic pigment in juice beverage and application method of rapid screening kit | |
| Yang et al. | Immunoaffinity column clean-up and liquid chromatography with post-column derivatization for analysis of aflatoxins in traditional Chinese medicine | |
| CN102928530A (en) | Detection method for measuring residual quantity of urea in bean sprout by high performance liquid chromatography | |
| Monakhova et al. | Rapid determination of coenzyme Q10 in food supplements using 1H NMR spectroscopy | |
| CN103913538B (en) | The quantitative detecting method of organophosphorus insecticide in a kind of tea fresh leaves | |
| CN106546724A (en) | A kind of new method of molecular beacon probe quick detection ochratoxin A | |
| CN103293119A (en) | Method for rapidly detecting contents of amaranthus red and brilliant blue in foods | |
| CN103760289A (en) | Extraction and high efficiency liquid-phase measurement method for anthocyanin in blood-flesh peach fruits | |
| Chen et al. | A rapid measurement of rutin-degrading enzyme activity in extract of tartary buckwheat seeds | |
| Nguyen et al. | Simultaneous determination of 11 water-soluble dyes in food products and beverages by high performance liquid chromatography | |
| CN101776659A (en) | Method for detecting anthocyanin in red radish through high-performance liquid chromatography | |
| Song et al. | Resonance Rayleigh scattering spectra, non-linear scattering spectra of malachite green-12-tungstophosphoric acid system and its analytical application in fish | |
| CN104483429B (en) | In Folium Camelliae sinensis, external source adds the fast and convenient detection method of 12 kinds of water solublity synthetic dyestuffs | |
| CN110208260A (en) | Potassium bromate quick detection reagent, kit and detection method in a kind of flour |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |