CN104535705A - Method for synchronously measuring capsaicin and capsorubin in capsicum oleoresin - Google Patents
Method for synchronously measuring capsaicin and capsorubin in capsicum oleoresin Download PDFInfo
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Abstract
The invention provides a method for synchronously measuring capsaicin and capsorubin in a capsicum oleoresin and belongs to the technical field of detection methods of the capsicum oleoresins. According to the method for synchronously measuring the capsaicin and capsorubin in the capsicum oleoresin, an isoabsorptive point graphing method is adopted, correction is performed by virtue of reversed phase high-performance liquid chromatography, the correction coefficient is introduced, and the content of the capsaicin and capsorubin in the capsicum oleoresin can be accurately, rapidly and synchronously measured. Therefore, the defect of dual wavelength spectrophotometry is overcome, the single wavelength direct colorimetric error is reduced, and the method is particularly applicable to the fields of tracking detection and quality analysis of products in industrial production.
Description
Technical field
The invention belongs to the detection method technical field of capsicum oleoresin, be specifically related to a kind of method of capsaicine and capsorubin in Simultaneous Determination capsicum oleoresin.
Background technology
Capsicum oleoresin is extraction from capsicum, the concentrated a kind of dark red oil liquid obtained, and color, smell and taste are all good, are the food additives raw materials of widespread use.The main active of capsicum oleoresin is capsaicine and capsorubin, the demand of domestic and international capsicum oleoresin is all very large, since Guizhou Chinese gall company limited in 1997 pushes to market it first time, annual sales is all being doubled and redoubled, and within 2003, China's sales volume is more than 100 tons.For detecting capsicum oleoresin product quality and determining best production process route, the quantitative analysis method of setting up reliable capsicum oleoresin just seems particularly important.Not yet form comparative maturity, easy, economic, reliable analytical approach at present, therefore, improving of capsicum oleoresin detection method is urgently to be resolved hurrily.
At present, the method measuring capsorubin and capsaicine mainly contains: GB10783-1996, FAO UV double colourimetry.In addition, sodium nitrite colourimetry, high performance liquid chromatography (HPLC), reversed-phased high performace liquid chromatographic (RP-HPLC) etc. can also be adopted to measure the content of capsorubin and capsaicine.HPLC and RP-HPLC instrument cost intensive, and complex operation, be not suitable for commercial introduction application, and other method once can only measure a kind of content of principal ingredient, also needs to carry out rough handling etc. to sample before mensuration.And because capsorubin and capsaicine combine, both separation are very difficult, measure wherein a kind of composition time, another kind of composition often causes interference to it.
In addition, the method for Simultaneously test binary mixture content has: Y-reference method, isobestic point graphing method.When absorption spectrum overlap is very serious when between component, can adopt Y-reference method, but capsorubin and capsaicine chemical constitution differ greatly, Duplication is not high, can not use Y-reference method.Consider that the potpourri absorption curve of two components has during intersection point and can adopt isobestic point graphing method, the present invention overcomes the deficiencies in the prior art, on the basis of existing technology, explore a kind of based on isobestic point graphing method, in conjunction with the method for the content of the capsorubin in RP-HPLC Simultaneously test capsicum oleoresin and capsaicine.
Summary of the invention
Technical matters to be solved by this invention is, for the deficiencies in the prior art, a kind of method of capsaicine and capsorubin in Simultaneous Determination capsicum oleoresin is provided, adopt isobestic point graphing method, correct with the result of RP-HPLC, introduce correction coefficient, accurately, fast, Simultaneous Determination goes out the content of capsaicine and capsorubin in capsicum oleoresin, both compensate for the deficiency of dual-wavelength spectrophotometry, again reduce the error of Single wavelength direct color comparison, be particularly suitable for tracing detection and the quality analysis field of product in commercial production.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is: a kind of method of capsaicine and capsorubin in Simultaneous Determination capsicum oleoresin, comprises the following steps:
The first step: utilize isobestic point graphing method to measure the content of capsaicine and capsorubin in capsicum oleoresin sample, be designated as C
1and C
2;
Second step: rp-hplc determination capsaicine standard items and capsorubin standard items, obtains the reversed-phase high-performance liquid chromatography spectrogram of capsaicine standard items and capsorubin standard items;
3rd step: the content of capsaicine and capsorubin in rp-hplc determination capsicum oleoresin sample, is designated as C
1 'and C
2 ';
4th step: the correction of capsaicine and capsorubin content in capsicum oleoresin sample, introduces correction coefficient alpha and β, then α=C respectively
1 '/ C
1, β=C
2 '/ C
2;
5th step: the content calculating capsaicine and capsorubin in capsicum oleoresin, the actual content obtaining capsaicine is α C
1, the actual content of capsorubin is β C
2.
The preparation method of described capsicum oleoresin sample is: take chilli powder, uses 75% ethanol, solid-to-liquid ratio 1:4(w/v), extract 4 hours at 40 DEG C, extract twice, extract merges, concentrated, be placed in the air dry oven of 40 DEG C, dry to constant weight, obtain capsicum oleoresin sample.
The described first step, utilizes isobestic point graphing method to measure the content of capsaicine and capsorubin in capsicum oleoresin sample, specifically comprises the following steps:
1. accurately take capsaicine standard items, be solvent, be mixed with standard solution with ethanol, concentration is 0.03mg/ml; Get capsorubin standard items, be solvent, be mixed with standard solution with ether, concentration is 0.03mg/ml; Then ultraviolet-visible spectrophotometer is utilized to carry out absorbance scanning to capsaicine standard solution and capsorubin standard solution respectively;
2. on the absorbance scintigram of capsorubin standard solution and capsaicine standard solution, common absorption point λ is found out
2, determine λ
1;
3. with (0,
) and (1,
), and (0,
) and (1,
) make absorbance and the relative concentration graph of a relation of capsorubin standard items and capsaicine standard items;
4. at absorption point λ
1and λ
2place measures the absorbance A of capsicum oleoresin sample respectively
λ 1and A
λ 2, then ask its ratio;
5. calculate the content of capsaicine and capsorubin in capsicum oleoresin sample, be designated as C
1and C
2.
Beneficial effect of the present invention is as follows:
1. the method for capsaicine and capsorubin in Simultaneous Determination capsicum oleoresin of the present invention, first the content of capsaicine and capsorubin is measured by isobestic point method, then corrected by RP-HLPC assay method, introduce correction coefficient, eliminate systematic error, final Accurate Determining goes out the actual content of capsaicine and capsorubin.Experimental cost of the present invention is cheap, easy and simple to handle, especially it can use common cheap mono-wavelength ultraviolet-visible spectrophotometer to analyze the content of the two kinds of materials co-existing in an individual system simultaneously, and without the need to introducing loaded down with trivial details isolation and purification process, so both compensate for the deficiency of Dual-Wavelength Spectrophotometric Determination, greatly reduce again the error of Single wavelength direct color comparison, and solve the high present situation of testing cost existing for high-performance liquid chromatogram determination method dexterously.
2. the present invention also comprises the preparation of capsicum oleoresin, make the present invention can measure capsorubin in self-control capsicum oleoresin and the content of capsaicine by isobestic point method, then corrected by RP-HLPC method, obtain capsorubin actual in capsicum oleoresin and the content of capsaicine, and then use as standard items, and without the need to again buying standard items, substantial saving in cost like this, method is easy, easy to implement.Like this when even without standard items, also can measure the capsorubin in capsicum oleoresin and capsaicine easily and accurately, be particularly suitable for tracing detection and the quality analysis of product in commercial production.
3. high performance liquid chromatography utilizes chromatography means, primary standard substance is effectively separated with impurities, be acknowledged as separating effect in existing various method in this way best, the method that accuracy of analysis is also the highest, but due to the fetch long price of instrument, regular job costly, complex operation, need special technician to test, limit its applying industrially.In the present invention, high performance liquid chromatography only plays the booster action of correcting measuring, determine correction coefficient in once testing after, then this experimental data can be reused in new products measure, and do not need frequently to utilize high performance liquid chromatography to measure, greatly reduce testing cost, be particularly suitable for tracing detection and the quality analysis field of product in commercial production.
The method of capsaicine and capsorubin in Simultaneous Determination capsicum oleoresin of the present invention, first isobestic point method is adopted to measure the content of capsaicine and capsorubin in capsicum oleoresin, then corrected by RP-HPLC method, introduce correction coefficient, obtain the actual content of capsorubin in capsicum oleoresin and capsaicine, be skillfully constructed, method is novel, easy, can the content of capsorubin quickly and accurately in Simultaneous Determination capsicum oleoresin and capsaicine.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the present invention is described in further detail.
Fig. 1: the reversed-phase high-performance liquid chromatography figure of capsaicine standard items;
Fig. 2: the reversed-phase high-performance liquid chromatography figure of self-control capsorubin standard items;
Fig. 3: the scanning curve figure of capsorubin and capsaicine standard items, A-capsaicine, B-capsorubin;
Fig. 4: the absorbance of capsorubin and capsaicine and relative concentration graph of a relation;
Fig. 5: the reversed-phase high-performance liquid chromatography figure of capsicum oleoresin.
Embodiment
The method of capsaicine and capsorubin in Simultaneous Determination capsicum oleoresin of the present invention, can carry out according to the following steps:
The first step: utilize isobestic point graphing method to measure the content of capsaicine and capsorubin in capsicum oleoresin sample
1) capsicum oleoresin sample is prepared
Take 25g chilli powder, use 75% ethanol, solid-to-liquid ratio 1:4(w/v), extract 4 hours at 40 DEG C, extract twice, extract merges, concentrated, is placed in the air dry oven of 40 DEG C, dries to constant weight, obtain capsicum oleoresin sample 5.3337g;
2) utilize isobestic point graphing method to measure the content of capsaicine and capsorubin in capsicum oleoresin sample, be designated as C
1and C
2;
1. accurately take capsaicine standard items, be solvent, be mixed with standard solution with ethanol, concentration is 0.03mg/ml; Get capsorubin standard items, be solvent, be mixed with standard solution with ether, concentration is 0.03mg/ml; Then ultraviolet-visible spectrophotometer is utilized to carry out absorbance scanning to capsaicine standard solution and capsorubin standard solution respectively;
2. on the absorbance scintigram of capsorubin standard solution and capsaicine standard solution, common absorption point λ is found out
2, determine λ
1;
3. with (0,
) and (1,
), and (0,
) and (1,
) make absorbance and the relative concentration graph of a relation of capsorubin standard items and capsaicine standard items;
4. at absorption point λ
1and λ
2place measures the absorbance A of capsicum oleoresin sample respectively
λ 1and A
λ 2, then ask its ratio;
5. calculate the content of capsaicine and capsorubin in capsicum oleoresin sample, be designated as C
1and C
2;
Second step: rp-hplc determination capsaicine standard items and capsorubin standard items, obtains the RP-HPLC spectrogram of capsaicine standard items and capsorubin standard items;
Take capsorubin standard items 0.2496g, be dissolved in 10.0ml hplc grade methanol, be mixed with the capsorubin standard solution of 0.2496g/ml; Take capsaicine standard items 0.18mg and be dissolved in the capsaicine standard solution that 5ml hplc grade methanol is mixed with 0.036mg/ml; Then high performance liquid chromatograph is utilized to measure;
3rd step: the content of capsaicine and capsorubin in rp-hplc determination capsicum oleoresin sample
1. take capsicum oleoresin sample 6.2mg and be dissolved in the solution being mixed with 1.24mg/ml in 5.0ml hplc grade methanol;
2. utilize high performance liquid chromatograph to measure capsicum oleoresin sample solution, obtain reversed-phase high-performance liquid chromatography spectrogram, calculate the content of capsaicine and capsorubin in capsicum oleoresin sample respectively, be designated as C
1 'and C
2 ';
4th step: the correction of capsaicine and capsorubin content in capsicum oleoresin sample
In conjunction with the measurement result of RP-HPLC method, the measurement result with isobestic point graphing method is contrasted, introduce correction coefficient alpha and β, then α=C respectively
1 '/ C
1, β=C
2 '/ C
2;
5th step: the content calculating capsaicine and capsorubin in capsicum oleoresin, the actual content obtaining capsaicine is α C
1, the actual content of capsorubin is β C
2.
In order to illustrate further in more detail, deepen the understanding of the present invention, instead of restriction the present invention, by reference to the accompanying drawings, provide following embodiment.
In the present embodiment, capsorubin is laboratory self-control, and is measured its purity by high performance liquid chromatograph, and concrete determination step is as follows:
Take homemade capsorubin 0.2496g, be dissolved in 10.0ml hplc grade methanol, be mixed with the capsorubin standard solution of 0.2496g/ml; Take capsaicine standard items 0.18mg and be dissolved in the capsaicine standard solution that 5ml hplc grade methanol is mixed with 0.036mg/ml; Then utilize high performance liquid chromatograph to measure, obtain the RP-HPLC spectrogram of capsaicine standard items and self-control capsorubin respectively, as depicted in figs. 1 and 2.
By comparison diagram 1 and Fig. 2, can find out, the capsaicine content in laboratory self-control capsorubin ten thousand/several, can ignore, so homemade capsorubin can use as standard items.
Therefore capsorubin standard items adopt the homemade capsorubin in laboratory in the present embodiment.
The first step: utilize isobestic point graphing method to measure the content of capsaicine and capsorubin in capsicum oleoresin sample
1) capsicum oleoresin sample is prepared
Take 25g chilli powder, use 75% ethanol, solid-to-liquid ratio 1:4(w/v), extract 4 hours at 40 DEG C, extract twice, extract merges, concentrated, is placed in the air dry oven of 40 DEG C, dries to constant weight, obtain capsicum oleoresin sample 5.3337g;
2) isobestic point graphing method measures the content of capsaicine and capsorubin in capsicum oleoresin sample
1. accurately take capsaicine standard items, be solvent, be mixed with standard solution with ethanol, concentration is 0.03mg/ml; Get capsorubin standard items, be solvent, be mixed with standard solution with ether, concentration is 0.03mg/ml; Then ultraviolet-visible spectrophotometer is utilized to carry out absorbance scanning to capsaicine standard solution and capsorubin standard solution respectively;
2. on the absorbance scintigram of capsorubin standard items and capsaicine standard solution, common absorption point λ is found out
2, determine λ
1;
;
Can obtain according to absorption law:
，
Wherein, A-absorbance, a-absorptivity, C-concentration;
Two formulas are divided by, then
;
Cause
, when A and B is isobestic point, then have
, and establish λ
2for isobestic point.
As shown in the capsorubin standard items of Fig. 3 and the scanning curve figure of capsaicine standard items, capsaicine and capsanthin have three intersection points, are respectively (242nm, 0.210), (263nm, 0.127), (292nm, 0.107).Consider from sensitivity, choose middle intersection point (larger or less all can cause comparatively big error) 263nm as λ
2.Capsaicine has larger absorption at wavelength 280nm place, and comparatively large in the receptivity difference of this wavelength place capsaicine and capsorubin, is the interference of both reductions, improves the sensitivity of measuring and select 280nm as λ
1.
3. absorbance and the relative concentration graph of a relation of capsorubin standard items and capsaicine standard items is made
Record capsaicine standard items and capsorubin standard items at λ
1(280nm) absorbance under is respectively
,
, carry it into formula
with
in,
,
;
Again, λ
2for isobestic point, so
,
If
,
, wherein, x, y represent relative concentration;
Make x=0,
=
=2.6096;
Make x=1 again,
=0.9456;
Then the absorbance of capsorubin standard items and capsaicine standard items and the graph of a relation of relative concentration is done with (0,0.9456) and (1,2.6096) and (0,2.6096) and (1,0.9456), as shown in Figure 4.
4. respectively at absorption point λ
1and λ
2the absorbance of lower mensuration capsicum oleoresin sample, and calculate the content of capsaicine and capsorubin in capsicum oleoresin sample
In wavelength X
2=263nm and λ
1=280nm place, tests the absorbance of capsicum oleoresin sample respectively, consults table 1, and calculate
/
.
The absorbance of table 1 capsicum oleoresin sample respectively when wavelength 263nm and 280nm
As table 1, the absorbance of capsicum oleoresin sample is at λ
1(280nm), λ
2(263nm) absorbance that place records sample is respectively: A
λ 1=1.227, A
λ 2=0.993, try to achieve ratio
=1.236, and the numerical value finding out corresponding x and y in the diagram, then basis
, try to achieve (
);
Substitute into formula:
with
;
Try to achieve
with
.
Then, then will
with
substitute into following formula:
The content C of capsaicine in capsicum oleoresin sample
1=C
a× V/m
The content C of capsorubin in capsicum oleoresin sample
2=C
b× V/m
In formula: C
athe content mg/ml of capsaicine in-capsicum oleoresin sample; V-liquor capacity ml; The quality g of the capsicum oleoresin sample that m-measures.
Result of calculation is: in capsicum oleoresin sample, the content of capsaicine and capsorubin is respectively C
1=7.28mg/g and C
2=17.29mg/g.
Second step: rp-hplc determination capsaicine standard items and capsorubin standard items, obtains the RP-HPLC spectrogram of capsaicine standard items and capsorubin standard items,
This step, front already described, repeats no more, and the RP-HPLC spectrogram of capsaicine standard items and capsorubin standard items as depicted in figs. 1 and 2.
3rd step: the content of capsaicine and capsorubin in rp-hplc determination capsicum oleoresin sample
1. take capsicum oleoresin sample 6.2mg and be dissolved in the solution being mixed with 1.24mg/ml in 5.0ml hplc grade methanol;
2. utilize high performance liquid chromatograph to measure capsicum oleoresin sample solution, obtain reversed-phase high-performance liquid chromatography spectrogram, as shown in Figure 5, composition graphs 1 and Fig. 2 calculate the content of capsaicine and capsorubin in capsicum oleoresin sample respectively, are designated as C
1 'and C
2 ';
As can be seen from Figure 1, the peak that retention time is about 3.47min should be the peak that the dissolvent residual of high polarity or other impurity are formed.Capsaicine has larger absorption at 280nm, and its retention time is 15.90min, and area integral is 663656, accounts for 63.26% of the total area; And last larger peak is dihydrocapsaicin, its retention time is 23.32min, and area integral is 246457, accounts for 23.49% of the total area.As can be seen from Fig. 5 composition graphs 1, retention time is the peak of 16.00min is capsaicine, and its area integral is 500696; And the peak that another retention time is 22.26 is dihydrocapsaicin, its area integral is 246958.
The area integral of capsaicine in composition graphs 1 and Fig. 5, and capsicum paper mill wastewater in capsaicine standard items, can calculate the content C of capsaicine in capsicum oleoresin
1 'for 13.85mg/g, the content of dihydrocapsaicin is 6.83mg/g.
Because homemade capsorubin is through purity testing, its purity is approximately 100%, does not adopt RP-HPLC to correct further to it in the present embodiment; In addition, also can carry out RP-HPLC mensuration to it, be designated as C
2 '.
4th step: the correction of capsaicine and capsorubin content in capsicum oleoresin sample
High performance liquid chromatography is adopted to correct the systematic error of isobestic point method, in order to evaluate the precision of the method.The content that reversed-phased high performace liquid chromatographic records capsaicine in capsicum oleoresin is 13.85mg/g, and the result that isobestic point method measures capsaicine is 7.28mg/g, 7.34mg/g and 7.22mg/g, its mean value is 7.28mg/g, and linearly better.In order to adopt more practical isobestic point graphing method, solving the systematic error problem that it is comparatively on the low side, introducing a correction coefficient, with reference to the result of RP-HPLC, this correction coefficient should be α=C
1 '/ C
1=13.85/7.28=1.90.
For capsorubin, said method also can be adopted to correct, introduce correction coefficient β, then β=C
2 '/ C
2; And in the present embodiment, because capsorubin is homemade, and verify that its purity is close to 100% through high performance liquid chromatography, without the need to correcting, or be interpreted as that correction coefficient is β=1.
5th step: the content calculating capsaicine and capsorubin in capsicum oleoresin
Actual capsaicine content should be the result C that isobestic point method calculates
1be multiplied by 1.90 times again, namely the actual content of capsaicine is C
1 '=α C
1=1.90 × 7.28mg/g=13.85mg/g;
The actual content of capsorubin is C
2 '=β C
2=1 × 17.29mg/g=17.29mg/g.
Adopt isobestic point graphing method of the present invention, then after correcting with RP-HPLC method, then without the need to repurchasing standard items.Experimental cost of the present invention is cheap, easy and simple to handle, and especially its available common cheap mono-wavelength ultraviolet-visible spectrophotometer is analyzed the content of the two kinds of materials co-existing in an individual system simultaneously, and without the need to introducing loaded down with trivial details isolation and purification.Both compensate for the deficiency of Dual-Wavelength Spectrophotometric Determination, again reduced the error of Single wavelength direct color comparison, be particularly suitable for tracing detection and the quality analysis of product in commercial production.Isobestic point graphing method corrects through RP-HPLC, can be used as a kind of very novel, easy, method of synchronously detecting capsaicine and capsorubin fast.
In sum, clearly, all any amendments done according to technical support essence of the present invention and change still belong in the scope that the technology of the present invention supports for every claim that in a kind of Simultaneous Determination of the present invention capsicum oleoresin, the method for capsaicine and capsorubin is addressed and technical support.
Claims (3)
1. the method for capsaicine and capsorubin in Simultaneous Determination capsicum oleoresin, is characterized in that, comprise the following steps:
The first step: utilize isobestic point graphing method to measure the content of capsaicine and capsorubin in capsicum oleoresin sample, be designated as C
1and C
2;
Second step: rp-hplc determination capsaicine standard items and capsorubin standard items, obtains the reversed-phase high-performance liquid chromatography spectrogram of capsaicine standard items and capsorubin standard items;
3rd step: the content of capsaicine and capsorubin in rp-hplc determination capsicum oleoresin sample, is designated as C
1 'and C
2 ';
4th step: the correction of capsaicine and capsorubin content in capsicum oleoresin sample, introduces correction coefficient alpha and β, then α=C respectively
1 '/ C
1, β=C
2 '/ C
2;
5th step: the content calculating capsaicine and capsorubin in capsicum oleoresin, the actual content obtaining capsaicine is α C
1, the actual content of capsorubin is β C
2.
2. the method for capsaicine and capsorubin in Simultaneous Determination capsicum oleoresin as claimed in claim 1, it is characterized in that, the preparation method of described capsicum oleoresin sample is: take chilli powder, uses 75% ethanol, solid-to-liquid ratio 1:4(w/v), extract 4 hours at 40 DEG C, extract twice, extract merges, concentrated, be placed in the air dry oven of 40 DEG C, dry to constant weight, obtain capsicum oleoresin sample.
3. the method for capsaicine and capsorubin in Simultaneous Determination capsicum oleoresin as claimed in claim 1, it is characterized in that: the described first step, utilize isobestic point graphing method to measure the content of capsaicine and capsorubin in capsicum oleoresin sample, specifically comprise the following steps:
1. accurately take capsaicine standard items, be solvent, be mixed with standard solution with ethanol, concentration is 0.03mg/ml; Get capsorubin standard items, be solvent, be mixed with standard solution with ether, concentration is 0.03mg/ml; Then ultraviolet-visible spectrophotometer is utilized to carry out absorbance scanning to capsaicine standard solution and capsorubin standard solution respectively;
2. on the absorbance scintigram of capsorubin standard solution and capsaicine standard solution, common absorption point λ is found out
2, determine λ
1;
3. with (0,
) and (1,
), and (0,
) and (1,
) make absorbance and the relative concentration graph of a relation of capsorubin standard items and capsaicine standard items;
4. at absorption point λ
1and λ
2place measures the absorbance A of capsicum oleoresin sample respectively
λ 1and A
λ 2, then ask its ratio;
5. calculate the content of capsaicine and capsorubin in capsicum oleoresin sample, be designated as C
1and C
2.
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| CN107656000A (en) * | 2017-10-26 | 2018-02-02 | 晨光生物科技集团股份有限公司 | The analysis and identification method of capsaicin compound in capsicum oleoresin |
| CN108181397A (en) * | 2017-12-29 | 2018-06-19 | 浙江农林大学 | Hangzhou chili capsaicine concentration extraction measuring method |
| CN108414665A (en) * | 2018-04-23 | 2018-08-17 | 河南科高中标检测技术有限公司 | The assay method of gingerol content in a kind of ginger medicinal material and its preparation |
| CN108414666A (en) * | 2018-04-23 | 2018-08-17 | 河南科高中标检测技术有限公司 | The assay method of gingerol content in a kind of ginger medicinal material extractive of volatile oil |
| CN112945879A (en) * | 2021-02-07 | 2021-06-11 | 四川省农业科学院园艺研究所 | Synergistic detection method for color value of capsaicin and capsorubin |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1105451A (en) * | 1994-01-15 | 1995-07-19 | 武汉大学 | Single-beam double-wavelength spectrophotometer |
| CN1587321A (en) * | 2004-08-26 | 2005-03-02 | 上海交通大学 | Process for separating capsorubin and capsaicine from hot pepper |
| CN101082609A (en) * | 2006-05-30 | 2007-12-05 | 田丽萍 | Method for detecting natural colouring matter pimiento red pigment in food product |
| CN102060728A (en) * | 2010-12-20 | 2011-05-18 | 重庆市农业科学院 | Method for extracting capsorubin and capsaicin from chillies |
-
2014
- 2014-12-23 CN CN201410815195.5A patent/CN104535705B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1105451A (en) * | 1994-01-15 | 1995-07-19 | 武汉大学 | Single-beam double-wavelength spectrophotometer |
| CN1587321A (en) * | 2004-08-26 | 2005-03-02 | 上海交通大学 | Process for separating capsorubin and capsaicine from hot pepper |
| CN101082609A (en) * | 2006-05-30 | 2007-12-05 | 田丽萍 | Method for detecting natural colouring matter pimiento red pigment in food product |
| CN102060728A (en) * | 2010-12-20 | 2011-05-18 | 重庆市农业科学院 | Method for extracting capsorubin and capsaicin from chillies |
Non-Patent Citations (8)
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107656000A (en) * | 2017-10-26 | 2018-02-02 | 晨光生物科技集团股份有限公司 | The analysis and identification method of capsaicin compound in capsicum oleoresin |
| CN107656000B (en) * | 2017-10-26 | 2021-04-09 | 晨光生物科技集团股份有限公司 | Method for analyzing and identifying capsaicin compounds in capsicum oleoresin |
| CN108181397A (en) * | 2017-12-29 | 2018-06-19 | 浙江农林大学 | Hangzhou chili capsaicine concentration extraction measuring method |
| CN108414665A (en) * | 2018-04-23 | 2018-08-17 | 河南科高中标检测技术有限公司 | The assay method of gingerol content in a kind of ginger medicinal material and its preparation |
| CN108414666A (en) * | 2018-04-23 | 2018-08-17 | 河南科高中标检测技术有限公司 | The assay method of gingerol content in a kind of ginger medicinal material extractive of volatile oil |
| CN112945879A (en) * | 2021-02-07 | 2021-06-11 | 四川省农业科学院园艺研究所 | Synergistic detection method for color value of capsaicin and capsorubin |
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