CN104524338B - A kind of Chinese medicine that treating IgA nephrosis and its application - Google Patents

A kind of Chinese medicine that treating IgA nephrosis and its application Download PDF

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CN104524338B
CN104524338B CN201510005056.0A CN201510005056A CN104524338B CN 104524338 B CN104524338 B CN 104524338B CN 201510005056 A CN201510005056 A CN 201510005056A CN 104524338 B CN104524338 B CN 104524338B
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chinese medicine
fruit
tortoiseshell
glossy privet
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CN104524338A (en
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陈以平
王琳
张春崧
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Longhua Hospital Affiliated to Shanghai University of TCM
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/80—Scrophulariaceae (Figwort family)
    • A61K36/804—Rehmannia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/58—Reptiles
    • A61K35/586—Turtles; Tortoises, e.g. terrapins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/284—Atractylodes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
    • A61K36/638—Ligustrum, e.g. Chinese privet
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/74—Rubiaceae (Madder family)
    • A61K36/748—Oldenlandia or Hedyotis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/894—Dioscoreaceae (Yam family)
    • A61K36/8945—Dioscorea, e.g. yam, Chinese yam or water yam
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
    • A61K36/8994—Coix (Job's tears)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
    • A61K9/0095—Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization

Abstract

The present invention relates to the Chinese medicine for the treatment of IgA nephrosis and its applications, and it includes the bulk pharmaceutical chemicals of following parts by weight: 9-15 parts of glutinous rehmannia, 9-15 parts of tortoiseshell, 9-15 parts of the fruit of glossy privet, 15-25 parts of Eclipta prostrata, 5-15 parts of Pollen Typhae, 12-18 parts of rhizoma atractylodis, 12-18 parts of Rhizoma Atractylodis Macrocephalae, 25-35 parts of oldenlandia diffusa, 25-35 parts of semen coicis, 10-18 parts of Chinese yam；Or 9-15 parts of glutinous rehmannia, 9-15 parts of tortoiseshell, 9-15 parts of the fruit of glossy privet, 15-25 parts of Eclipta prostrata, 25-35 parts of oldenlandia diffusa, 5-15 parts of Pollen Typhae.Drug matching of the invention is appropriate, has the effect of nourshing kidney invigorating the spleen, clearing heat and nourishing yin, and CD can be effectively reduced44It in the deposition of glomerulus, therefore can be used to treat IgA nephrosis, delay the progress of IgA nephrosis, while the Chinese medicine flavour of a drug number is few, it is at low cost convenient for preparation, and be made of pure Chinese medicine, it has no toxic side effect, is easy to be accepted by patients.

Description

A kind of Chinese medicine that treating IgA nephrosis and its application
[technical field]
The present invention relates to a kind of Chinese medicine for treating IgA nephrosis and its applications, specifically, being one kind using Chinese herbal medicine as raw material The Chinese patent drug of preparation.
[background technique]
IgA nephrosis (IgA nephropathy, IgAN) is one group of clinical syndrome with co-immunization pathological characters, Its main feature is that based on nephridial tissue immunopathogenesis checks that glomerular mesangium area deposits with IgA, along with different degrees of focal or Diffusivity mesangial matrix *.IgA nephrosis is a kind of progressive disease, and being increasingly becoming leads to China's end-stage renal disease (ESRD) most One of common disease factor is the first protopathy of the chronic maintenance hemodialysis in China.Doctor trained in Western medicine pays attention to the morbidity to IgA nephrosis more The research of mechanism, histological change, but in terms for the treatment of, doctor trained in Western medicine still lacks effective method and means.Recently as Chinese medicine It deepens continuously to IgA nephrosis research, the prospect that traditional Chinese medicine treats the disease attracts attention.
Chinese patent literature CN201110364252.9, publication date 2012.03.14 disclose a kind of IgA nephrosis for the treatment of Chinese traditional compound medicine, including following weight proportion Chinese medicine material: Radix Astragali 13-17, Radix Rehmanniae 8-12, Radix Salviae Miltiorrhizae 8-12, bletilla 8-12, Cicada slough 2-4, garden burnet 8-12, rhizoma imperatae 16-24, cordyceps sinensis (punching) 2-4；Preparation method is to clean above-mentioned 8 taste medicinal material, is pressed Ratio mixing, the ethyl alcohol that 6-8 times of the water or volume ratio of dosing material total weight are 70% decoct 3 times, 40 minutes every time, merge 3 Secondary decoction liquor, filtering, and be concentrated, the liquid extract that specific gravity is 1.21 at 60 DEG C is obtained, is made any one using conventional fabrication process Described dosage form in kind pharmacy, such as tablet, capsule, granule, oral solution, pill, syrup；Herbal mixture system obtained Agent greatly reduces the albuminuria of IgA nephrotic and the concentration of blood urine, substantially improves renal function, significant in efficacy, subtracts significantly Side effect and adverse reaction are lacked.
Chinese periodical " Chinese combination of Chinese tradiational and Western medicine nephrosis magazine " the 10th phase of volume 8 in October, 2007, " kidney is flat for the paper published Particle is to IgA nephrosis kidney of mouse CD44Influence ", inquired into the mechanism of action of the flat granule therapy Experimental IgA Nephropathy of kidney, The flat particle of the kidney is made of Radix Rehmanniae, the fruit of glossy privet, tortoiseshell, Chinese yam, Pollen Typhae, eclipta, Rhizoma Atractylodis Macrocephalae, rhizoma atractylodis, semen coicis etc., is made Person is immunized composite algorithm using oral bovine serum albumin(BSA) (BSA) and tail vein injection staphylococcal enterotoxin B (SEB) and induces mouse IgA nephropathy model, is randomly divided into normal group, model group, Lotensin group and the flat particle low, middle and high dose groups of kidney, and totally 6 groups.Observation Pretherapy and post-treatment deposition of the IgA immune complex in nephridial tissue, detects CD by Immunohistochemical Method44In the expression of nephridial tissue.Knot Fruit: normal group is deposited without IgA, and IgA deposition occurs in model group glomerulus, and treatment group IgA glomerulus deposition compared with model Group is substantially reduced (P < 0.01), and normal group only has a small amount of CD44It expresses, CD in model group44Expression be remarkably reinforced, treatment group (removes The flat low dose group of kidney) CD44It is significantly reduced in the expression of glomerulus, and is in dose-dependence in each treatment group of the flat particle of kidney (P<0.01).Conclude that the flat particle of kidney can reduce IgA nephrosis kidney of mouse CD44Expression, to delay IgA nephrosis Progress.
However the flavour of a drug number of the Chinese traditional compound medicine of above-mentioned treatment IgA nephrosis and the flat particle of Chinese medicine kidney is more, compatibility is not It is enough precise and appropriate, therefore need a kind of Chinese medicine composition that compatibility is more precise and appropriate.Meanwhile the more Chinese prescriptions that curative effect is better of research and development It is also very necessary.
[summary of the invention]
The purpose of the present invention is aiming at the shortcomings in the prior art, provide a kind of Chinese medicine for treating IgA nephrosis.
Another purpose of the invention is to provide the purposes of the Chinese medicine.
To realize above-mentioned first purpose, a kind of technical solution that the present invention takes is: a kind of to treat in IgA nephrosis Medicine, it includes the bulk pharmaceutical chemicals of following parts by weight: 9-15 parts of glutinous rehmannia, 9-15 parts of tortoiseshell, 9-15 parts of the fruit of glossy privet, 15-25 parts of Eclipta prostrata, life 5-15 parts of cattail pollen, 12-18 parts of rhizoma atractylodis, 12-18 parts of Rhizoma Atractylodis Macrocephalae, 25-35 parts of oldenlandia diffusa, 25-35 parts of semen coicis, Chinese yam 10-18 Part.
The Chinese medicine includes the bulk pharmaceutical chemicals of following parts by weight: 10-14 parts of glutinous rehmannia, 10-14 parts of tortoiseshell, fruit of glossy privet 10-14 Part, 16-20 parts of Eclipta prostrata, 8-11 parts of Pollen Typhae, 14-16 parts of rhizoma atractylodis, 14-16 parts of Rhizoma Atractylodis Macrocephalae, 28-32 parts of oldenlandia diffusa, semen coicis 28-32 parts, 14-16 parts of Chinese yam.
The Chinese medicine includes the bulk pharmaceutical chemicals of following parts by weight: 12 parts of glutinous rehmannia, 12 parts of tortoiseshell, 12 parts of the fruit of glossy privet, Eclipta prostrata 20 Part, 10 parts of Pollen Typhae, 15 parts of rhizoma atractylodis, 15 parts of Rhizoma Atractylodis Macrocephalae, 30 parts of oldenlandia diffusa, 30 parts of semen coicis, 15 parts of Chinese yam.
The another technical solution that the present invention takes is: a kind of Chinese medicine for treating IgA nephrosis, it includes following parts by weight Bulk pharmaceutical chemicals: 9-15 parts of glutinous rehmannia, 9-15 parts of tortoiseshell, 9-15 parts of the fruit of glossy privet, 15-25 parts of Eclipta prostrata, 25-35 parts of oldenlandia diffusa, raw Pu It is 5-15 parts yellow.
The Chinese medicine includes the bulk pharmaceutical chemicals of following parts by weight: 10-14 parts of glutinous rehmannia, 10-14 parts of tortoiseshell, fruit of glossy privet 10-14 Part, 16-20 parts of Eclipta prostrata, 28-32 parts of oldenlandia diffusa, 8-11 parts of Pollen Typhae.
The Chinese medicine includes the bulk pharmaceutical chemicals of following parts by weight: 12 parts of glutinous rehmannia, 12 parts of tortoiseshell, 12 parts of the fruit of glossy privet, Eclipta prostrata 18 Part, 30 parts of oldenlandia diffusa, 9 parts of Pollen Typhae.
As above the dosage form of any Chinese medicine can be capsule, granule, tablet or mixture.
To realize above-mentioned second purpose, the technical solution adopted by the present invention is that:
As above application of any Chinese medicine in the drug of preparation treatment IgA nephrosis.
It should be noted that the fruit of glossy privet is the drying of Oleaceae plants glossy privet Ligustrum lucidum Ait. Ripening fruits.The Pollen Typhae is that cattail pollen is rubbed to broken agglomeration, and sieving removes impurity to obtain the final product.
The invention has the advantages that:
1, Chinese medicine one of the invention and the equal compatibility of Chinese medicine two are appropriate, have the effect of nourshing kidney invigorating the spleen, clearing heat and nourishing yin, can be effective Reduce CD44It in the deposition of glomerulus, therefore can be used to treat IgA nephrosis, delay the progress of IgA nephrosis；
2, one flavour of a drug number of Chinese medicine of the invention is few, and convenient for preparation, abundant raw materials are easy to get, at low cost, is suitable for promoting；
3, it is made, has no toxic side effect of pure Chinese medicine, be easy to be accepted by patients.
[specific embodiment]
It elaborates below to specific embodiment provided by the invention.
Herein, conventional method decoction is the production method of traditional Chinese herbal decoction routine, i.e., adds the bulk pharmaceutical chemicals Decocting is cooked into decoction.
The preparation (one) of the Chinese medicine one of the invention of embodiment 1
It is 12 parts of glutinous rehmannia, 12 parts of tortoiseshell, 12 parts of the fruit of glossy privet, 20 parts of Eclipta prostrata, 10 parts of Pollen Typhae, 15 parts of rhizoma atractylodis, 15 parts of Rhizoma Atractylodis Macrocephalae, white Flower 30 parts of HERBA HEDYOTIS DIFFUSAE, 30 parts of semen coicis, 15 parts of Chinese yam, conventional method decoct.
The preparation (two) of the Chinese medicine one of the invention of embodiment 2
It is 15 parts of glutinous rehmannia, 9 parts of tortoiseshell, 15 parts of the fruit of glossy privet, 15 parts of Eclipta prostrata, 15 parts of Pollen Typhae, 12 parts of rhizoma atractylodis, 18 parts of Rhizoma Atractylodis Macrocephalae, white Flower 25 parts of HERBA HEDYOTIS DIFFUSAE, 35 parts of semen coicis, 10 parts of Chinese yam, conventional method decoct.
The preparation (three) of the Chinese medicine one of the invention of embodiment 3
9 parts of glutinous rehmannia, 15 parts of tortoiseshell, 9 parts of the fruit of glossy privet, 25 parts of Eclipta prostrata, 5 parts of Pollen Typhae, 18 parts of rhizoma atractylodis, 12 parts of Rhizoma Atractylodis Macrocephalae, white flower 35 parts of HERBA HEDYOTIS DIFFUSAE, 25 parts of semen coicis, 18 parts of Chinese yam, conventional method decoct.
The preparation (four) of the Chinese medicine one of the invention of embodiment 4
9 parts of glutinous rehmannia, 9 parts of tortoiseshell, 15 parts of the fruit of glossy privet, 25 parts of Eclipta prostrata, 5 parts of Pollen Typhae, 12 parts of rhizoma atractylodis, 18 parts of Rhizoma Atractylodis Macrocephalae, white flower 35 parts of HERBA HEDYOTIS DIFFUSAE, 25 parts of semen coicis, 10 parts of Chinese yam, conventional method decoct.
The preparation (five) of the Chinese medicine one of the invention of embodiment 5
It is 15 parts of glutinous rehmannia, 15 parts of tortoiseshell, 15 parts of the fruit of glossy privet, 15 parts of Eclipta prostrata, 5 parts of Pollen Typhae, 12 parts of rhizoma atractylodis, 18 parts of Rhizoma Atractylodis Macrocephalae, white Flower 35 parts of HERBA HEDYOTIS DIFFUSAE, 35 parts of semen coicis, 18 parts of Chinese yam, conventional method decoct.
The preparation (six) of the Chinese medicine one of the invention of embodiment 6
It is 10 parts of glutinous rehmannia, 14 parts of tortoiseshell, 10 parts of the fruit of glossy privet, 20 parts of Eclipta prostrata, 8 parts of Pollen Typhae, 16 parts of rhizoma atractylodis, 14 parts of Rhizoma Atractylodis Macrocephalae, white Flower 32 parts of HERBA HEDYOTIS DIFFUSAE, 28 parts of semen coicis, 16 parts of Chinese yam, conventional method decoct.
The preparation (seven) of the Chinese medicine one of the invention of embodiment 7
It is 14 parts of glutinous rehmannia, 10 parts of tortoiseshell, 14 parts of the fruit of glossy privet, 16 parts of Eclipta prostrata, 11 parts of Pollen Typhae, 14 parts of rhizoma atractylodis, 16 parts of Rhizoma Atractylodis Macrocephalae, white Flower 28 parts of HERBA HEDYOTIS DIFFUSAE, 32 parts of semen coicis, 14 parts of Chinese yam, conventional method decoct.
The preparation (eight) of the Chinese medicine one of the invention of embodiment 8
It is 9 parts of glutinous rehmannia, 15 parts of tortoiseshell, 15 parts of the fruit of glossy privet, 25 parts of Eclipta prostrata, 15 parts of Pollen Typhae, 12 parts of rhizoma atractylodis, 12 parts of Rhizoma Atractylodis Macrocephalae, white Flower 25 parts of HERBA HEDYOTIS DIFFUSAE, 35 parts of semen coicis, 10 parts of Chinese yam, conventional method decoct.
The preparation (one) of the Chinese medicine two of the invention of embodiment 9
12 parts of glutinous rehmannia, 12 parts of tortoiseshell, 12 parts of the fruit of glossy privet, 18 parts of Eclipta prostrata, 30 parts of oldenlandia diffusa, 9 parts of Pollen Typhae, routine side Method decocts.
The preparation (two) of the Chinese medicine two of the invention of embodiment 10
9 parts of glutinous rehmannia, 15 parts of tortoiseshell, 9 parts of the fruit of glossy privet, 25 parts of Eclipta prostrata, 25 parts of oldenlandia diffusa, 15 parts of Pollen Typhae, routine side Method decocts.
The preparation (three) of the Chinese medicine two of the invention of embodiment 11
15 parts of glutinous rehmannia, 9 parts of tortoiseshell, 15 parts of the fruit of glossy privet, 15 parts of Eclipta prostrata, 35 parts of oldenlandia diffusa, 5 parts of Pollen Typhae, routine side Method decocts.
The preparation (four) of the Chinese medicine two of the invention of embodiment 12
9 parts of glutinous rehmannia, 9 parts of tortoiseshell, 15 parts of the fruit of glossy privet, 25 parts of Eclipta prostrata, 25 parts of oldenlandia diffusa, 5 parts of Pollen Typhae, conventional method It decocts.
The preparation (five) of the Chinese medicine two of the invention of embodiment 13
15 parts of glutinous rehmannia, 15 parts of tortoiseshell, 9 parts of the fruit of glossy privet, 15 parts of Eclipta prostrata, 35 parts of oldenlandia diffusa, 15 parts of Pollen Typhae, routine side Method decocts.
The preparation (six) of the Chinese medicine two of the invention of embodiment 14
10 parts of glutinous rehmannia, 14 parts of tortoiseshell, 10 parts of the fruit of glossy privet, 20 parts of Eclipta prostrata, 28 parts of oldenlandia diffusa, 11 parts of Pollen Typhae, it is conventional Method decocts.
The preparation (seven) of the Chinese medicine two of the invention of embodiment 15
14 parts of glutinous rehmannia, 10 parts of tortoiseshell, 14 parts of the fruit of glossy privet, 16 parts of Eclipta prostrata, 32 parts of oldenlandia diffusa, 8 parts of Pollen Typhae, routine side Method decocts.
The preparation (eight) of the Chinese medicine two of the invention of embodiment 16
9 parts of glutinous rehmannia, 14 parts of tortoiseshell, 12 parts of the fruit of glossy privet, 20 parts of Eclipta prostrata, 25 parts of oldenlandia diffusa, 5 parts of Pollen Typhae, routine side Method decocts.
The preparation of the Chinese medicinal tablet/capsule of the present invention of embodiment 17
Any Chinese herb of Example 1-16, adds 8-12 times to measure water, decocts 1-3 hours, filter out decoction.Again Add 10 times of amount water, decoct 1.5-2.5 hours, filter out decoction, merge secondary decocting liquid, stand, leaching supernatant, concentration lets cool, adds 2.5 times of amount alcohol of concentrate stir precipitates overnight.Supernatant is taken, thick medicinal extract is concentrated into；Pharmaceutical aids, vacuum drying, powder is added Broken granulation, tabletted or filling are encapsulated.
The preparation of the Chinese herbal granules of the present invention of embodiment 18
Any Chinese herb of Example 1-16, adds 8-10 times to measure water, decocts 2.5-3.5 hours, filter out medicine Juice.Again plus 10 times of amount water, decoction 2 hours filter out decoction, merge secondary decocting liquid, stand, leaching supernatant, concentration lets cool, adds 2 times of amount alcohol of concentrate stir precipitates overnight.Supernatant is taken, thick medicinal extract is concentrated into；Add appropriate pharmaceutical aids, pelletizes, it is dry, Whole grain obtains 20g particle, dispenses 10g/ bags.
The preparation of the herb mixture of the present invention of embodiment 19
Any Chinese herb of Example 1-16, adds 8-10 times to measure water, decocts 3 hours, filter out decoction.Again plus 8 times of amount water decoct 2 hours, filter out decoction, merge secondary decocting liquid, stand, leaching supernatant, and concentration lets cool, adds 2 times of concentrate Alcohol is measured, precipitates overnight is stirred.Supernatant is taken, thick medicinal extract is concentrated into；Add appropriate pharmaceutical aids, mixture is made.
The animal experiment of the Chinese medicine of the present invention of embodiment 20
1 experimental material
1.1 drugs and reagent
Bovine serum albumin(BSA) (BSA): AMRESCO company (lot number 0409A09).Staphylococcal enterotoxin B (SEB): 1mg/ Branch, Beijing Microbiology and Epidemic Disease Inst., Academy of Military-Medical Sciences (lot number 04-1-9).The flat particle of kidney: 12g/ bags, Shanghai Chinese medicine The production of the commission Shanghai Xiu Long pharmaceutical factory of traditional Chinese medicine, attached Longhua hospital, medicine university (by Radix Rehmanniae 10g, fruit of glossy privet 10g, tortoise plastron 10g, Chinese yam 30g, Pollen Typhae 20g, eclipta 20g, Rhizoma Atractylodis Macrocephalae 10g, rhizoma atractylodis 10g, semen coicis 30g composition).Lotensin: 10mg/ piece, Beijing Novartis system Medicine Co., Ltd (lot number 04128).The granule of Chinese medicine one of the present invention: it is prepared into and the flat particle of kidney according to the prescription of embodiment 1 The granule of isoreactivity component content.The granule of Chinese medicine two of the present invention: it is prepared into and the flat particle of kidney according to the prescription of embodiment 9 The granule of isoreactivity component content.Fluorescein isothiocynate (FITC) marks rabbit anti-mouse IgA antibody, Rocklang company (lot number 2520)；Mouse CD44Monoclonal antibody: Santa Crus company (lot number L1003).
1.2 key instrument
80-2 centrifugation device, 7170 type automatic analyzer of Hitachi, LEICA CM1850 freezing microtome, Nikon E600 Fluorescence microscope, Nikon IX71 inversion type research microscope.
1.3 experimental animal
6-7 week old male BALB/C mice 107, weight 16-18g, Shanghai western Poole-Bi Kai experimental animal Co., Ltd It provides.
2 experimental methods
2.1 animal packets and modeling
The preparation of IgA nephropathy model is referring to (Huang Wenzheng, Ma Hongmei bovine serum albumin(BSA) and the staphylococcus intestines poison such as Huang Wenzheng Element induces research the 12nd national Chinese Medical Nephrosis academic meeting paper compilation of mouse IgA nephrosis, 1996:120-122.) report The method in road first raises above-mentioned mouse in cleaning grade animal house, and precuring has urine examined routine after 1 week, selects Urine proteins and red blood cell It is negative mice 54.Taking 8 at random is normal group, and 0.1% bovine serum albumin(BSA) (BSA) acid is taken orally the next day remaining mouse is equal Change water (6mM hydrochloric acid), until putting to death day.Modeling mouse timing tail vein injection 1%BSA buffer 0.4ml/ at the 6th week, daily 1 time, continuous 3d；Tail vein injection staphylococcal enterotoxin B (SEB), dosage 0.8mgkg from 9th week-1Week-1, weekly 1 It is secondary, continuous 3 weeks.Normal group free water, conventinal breeding, until putting to death day.46 mouse are randomly divided by weight at the 9th week Two groups, the flat particle group of kidney 16, one group 15, Chinese medicine of the present invention, two groups 15, Chinese medicine of the present invention.
2.2 medication
Each pharmaceutical intervention group mouse gives corresponding drug stomach-filling respectively, one time a day, totally 5 weeks.The flat particle group of kidney, the present invention Two groups of one group of Chinese medicine, Chinese medicine of the present invention equal 9.6gkg-1·d-1(dosage be equivalent to adult human dose 20 times).It is each in 14 weeks Group animal ad lib, the next day free water.
3 detection projects and method
3.1 kidney function test
Serum creatinine (Scr), urea nitrogen (BUN) measurement, are detected using 7170 type automatic analyzer of Hitachi.
3.2 immunofluorescences (IF) observation
Kidney of mouse is taken, row OCT is embedded at once, and the frozen section of 5um thickness is made, carries out using direct immunofluorescence IgA dyeing, observation glomerulus is interior, and whether there is or not IgA deposition, the distribution of double-blind study record and intensity.It is strong according to its fluorescence under fluorescence microscope Degree is divided into: (-) unstressed configuration；(±) fluorescence is very weak；(+) fluorescence is weaker, but clear；(++) fluorescence is bright；(+++) fluorescence is very strong.
3.3 Immunohistochemical detection
Paraffin section is taken, using ABC method row CD44Immunohistochemical staining, takes 3um thickness paraffin section, dewaxing aquation, 1.5% hydrogen peroxide-methanol solution blocks endogenous peroxydase 30min, sets 30min in 0.1% pancreatin buffer and carries out Antigen retrieval is added dropwise 5% notmal horse sera 20min and closes heterogenetic antigen, then with 5% 1 anti-mouse CD44Monoclonal is anti- Body is incubated for 4 DEG C of refrigerator overnights of 60min juxtaposition, and next day takes out, and 1% secondary antibody horse anti-mouse IgG antibody is added dropwise and is incubated for 60min, is added dropwise ABC reagent 50min, after develop the color and methyl green counterstain nucleus with 0.2% in 3,3 '-diaminobenzidines (DAB), drying, Mounting.Positive as the result is shown is in sepia, and nucleus is in light green.In all samples under the microscope, each sample takes 10 big Small similar glomerulus, according to software analysis (the cellular immunity immunohistochemical assay that East China University of Science develops, version: V3.0for Windows) each glomerulus immunohistochemical staining positive area accounts for the ratio of entire glomerular filtration area, counts every group of immunohistochemistry Positive area percentage.
4 statistical methods
Using 11.0 for Windows of SPSS Version carry out data statistics, measurement data withIt indicates, Compare between multiple groups data and analyzed using One-Way ANOVA, the comparison of class count data uses rank sum test.
5 experimental results
The detection of 5.1 renal functions
After observation treatment the case where each group renal function, compare creatinine, urea nitrogen between each group, no difference of science of statistics.It is shown in Table 1.
1 each group Mouse Kidney Function detection situation of table
Group n Scr(μmol/L) BUN(mmol/L)
Normal group 8 28.80±2.52 8.83±1.49
The flat particle group of kidney 16 30.03±3.78 8.81±1.21
One group of Chinese medicine of the present invention 15 29.92±3.54 7.98±1.23
Two groups of Chinese medicine of the present invention 15 28.79±3.23 7.07±1.06
The observation of 5.2 immunofluorescences
Only there is IgA deposition (±) in individual mice in normal group glomerular mesangium area, remaining mouse is feminine gender；In the present invention One group of medicine, two groups of Chinese medicine of the present invention have statistical difference (P < 0.05) compared with the flat particle group of kidney respectively, are shown in Table 2.
2 each group mouse IgA Immunofluorescence test situation of table
5.3 immunohistochemical observation
Normal group kidney of mouse has a small amount of CD44Positive brown dyeing, is distributed mainly on glomerular mesangium area, capillary Button loop and tubulo-interstital position；One group of Chinese medicine of the present invention, two groups of the Chinese medicine of the present invention equal no difference of science of statistics compared with normal group respectively (P>0.05) has statistical difference (P<0.05) compared with the flat particle group of kidney respectively, is shown in Table 3.
3 CD of table44It dyes glomerular matrix positive area ratio (%)
Group n CD44
Normal group 6 32.04±9.50#
The flat particle group of kidney 6 51.98±12.90*
One group of Chinese medicine of the present invention 6 33.71±12.34#
Two groups of Chinese medicine of the present invention 6 37.25±10.17#
Note: compared with normal group, P < 0.05 *；Compared with the flat particle group of kidney, #P < 0.05.
In conclusion Chinese medicine one and Chinese medicine two of the invention can effectively reduce CD44In the expression of glomerulus, improves and make It with fairly obvious, therefore can be used for treating IgA nephrosis, delay the progress of IgA nephrosis.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art Member, under the premise of not departing from the method for the present invention, can also make several improvement and supplement, these are improved and supplement also should be regarded as Protection scope of the present invention.

Claims (2)

1. a kind of Chinese medicine for treating IgA nephrosis, which is characterized in that it is prepared from the following raw materials in parts by weight: 12 parts of glutinous rehmannia, tortoise 12 parts of plate, 12 parts of the fruit of glossy privet, 18 parts of Eclipta prostrata, 30 parts of oldenlandia diffusa, 9 parts of Pollen Typhae；Conventional method decocts, and is prepared into particle Agent.
2. application of the Chinese medicine described in claim 1 in the drug of preparation treatment IgA nephrosis.
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Title
shengshumeng.肾内协定方准确版.《百度文库》.2013,第1页. *
中医及中西医结合治疗IgA肾病疗效分析;须冰等;《中国中西医结合肾病杂志》;20110430;第12卷(第4期);第333-335页 *
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肾平颗粒对1-磷酸鞘氨醇刺激大鼠肾小球系膜细胞增生的调节作用研究;钟逸斐;《中国中西医结合肾病杂志》;20120930;第13卷(第9期);第758-761页 *
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