CN1562168A - Compound preparation of cooling blood and promoting blood flow for curing psoriasis, and preparation method - Google Patents
Compound preparation of cooling blood and promoting blood flow for curing psoriasis, and preparation method Download PDFInfo
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Abstract
A Chinese medicine for treating psoriasis and its prparing process are disclosed. Its advantages are high curative effect and no toxic by-effect.
Description
Technical field
The invention belongs to the screening and the preparing technical field of Chinese medicinal formulae.
The present invention adopts used advanced technology of modern pharmacodynamic study and method, all are treated that psoriatic Chinese medicine screens and optimize prescription by the traditional Chinese medical science rule of treatment, through testing sieve examination and clinical verification repeatedly, draw the effective removing heat from blood and promoting blood circulation compound recipe of clinical treatment psoriasis at last.Verified the feasibility of used experimental model and detection index.The removing heat from blood and promoting blood circulation compound recipe action temperature that research obtains and, do not see that toxic and side effects can be widely used in all types hospital.Further make electuary, pill and also can be used for pharmacy.Further exploitation also can enter the international market.This experimental model determined and detection index can be used for tcm development.
Background technology
Psoriasis is a commonly encountered diseases, frequently-occurring disease.China's sickness rate is 0.12%, and American-European sickness rate can reach 1-3%.Psoriatic treatment does not still have active drug at present, the progress of western medical treatment psoriasis innerlich anwenden object space face comprises cell toxicity medicament, retinoid medicine and immunosuppressant-regulator since the fifties, but curative effect all serious side reaction may take place than the powerhouse in these medicines.Another important therapeutic advance is light-chemotherapy, and it is evident in efficacy, but has potential carcinogenic danger.The psoriatic cause of disease is not clear fully as yet, and primary disease easily recurs, Gu need to repeat even long-term treatment.Psoriasis is again a kind of optimum dermatosis, not should be temporary transient alleviation in the treatment and emits the danger that serious side effects takes place.The immediate objective of curing psoriasis research is according to pathogenetic understanding, seeks curative effect and does not have serious side effects really, recurs less and medicine and therapy that remission time is long.The Chinese medicine action temperature and, have no side effect, be fit to prolonged application.The Chinese traditional treatment psoriasis has accumulated rich experiences for many years, should be put in order and study.The Scientific exploration detection means judges that the relation of herbal medicine efficacy and the different Chinese medicine rule of treatment and prescription and experiment drug effect is a break-through point of tcm development.Can further develop the psoriatic more effective prescription of clinical treatment based on this.Also, Chinese medicine lays the foundation for squeezing into the international market.
Chinese medicine is distinct Chinese characteristics, and Chinese medicine research in the past uses traditional method, is main treatment foundation with dialectical, the rule of treatment, and observation of curative effect is confined to clinical indices, lacks the detection means of science.Serological index such as domestic in recent years existing human ET-1, IL-8, thromboxane B2,6-ketone-Prostaglandin F2a detect the psoriatic curative effect of treatment by Chinese herbs.But be the observation of numerical example clinical efficacy only, sample is little, mostly is single index, no zoopery foundation.
Psoriatic main pathology is changed to the epidermis hyperplasia and differentiation is incomplete.The estrogen phase outgrowth mouse vagina epithelium model of Bonder and Van Scott can be simulated psoriatic hyperproliferative epidermal.It is incomplete that the Mus tail scale epidermis model of Jarrett can be simulated psoriatic epidermal differentiation.Be to generally acknowledge at present both at home and abroad and use more psoriasis experimental model.Be used for the research of curative effect of medication more.But be used for the Chinese medicine sieve and try not appear in the newspapers as yet announcement both at home and abroad.PCNA is the best index of reflection propagation now, is used for the research of tumor more, the announcement but the index that the introducing animal model tries as the pharmacodynamics sieve is not appeared in the newspapers both at home and abroad as yet.Modern notion thinks that psoriatic hyperproliferative epidermal and disease phenomenons such as disdifferentiation and inflammation relate to the unusual regulation and control of cell and molecular level, show as cytokine, immune molecule and inflammation meson that skin lesion and blood dyscrasia are expressed, the order of severity of some and disease is closely related.External this aspect research is more, thinks that at present the index relevant with the psoriasis state of an illness has IL-8, ET-1, Se-selectin, NO, mopterin etc.These researchs mostly are clinical observation, and Se-selectin introduces the experimental model announcement that do not appear in the newspapers as yet both at home and abroad as detecting index.
Summary of the invention purpose of the present invention just provides a kind ofly can effectively treat psoriasis, have no side effect, be fit to life-time service be used for the treatment of psoriatic removing heat from blood and promoting blood circulation compound recipe and preparation method thereof.
Technical conceive of the present invention is, from the modern pharmacodynamics R and D treatment by Chinese herbs psoriasis of starting with.Research adopts animal model, SABC, hematology's detection, cell in vitro to cultivate many index and combine, examination of a large amount of zoopery sieve and clinical observation on the therapeutic effect combine, and the science detection means and the traditional Chinese medical science logos rule of treatment combine and develop the psoriatic Chinese medicinal formulae of treatment.
At first reported literature is treated the side's of tearing open the analysis of psoriatic Chinese medicinal formulae, pick out the high Chinese medicine of the frequency of occurrences.Utilize mouse vagina epithelium and Mus tail scale epidermis model, proliferating cell nuclear antigen expression, cytokine and methods such as inflammatory mediator detection and cytologic experiment that these Chinese medicines are sieved examination repeatedly, obtain 20 kinds of Chinese medicines such as Radix Arnebiae (Radix Lithospermi), Folium Isatidis, Herba Schizonepetae and can suppress epithelial cell proliferation to some extent, promote the epidermis cell differentiation and reduce endothelin level-1 (ET-1) level.We form 6 prescriptions with 20 flavor Chinese medicines by the traditional Chinese medical science rule of treatment again and further sieve examination on this basis, obtain by removing heat from blood, invigorate blood circulation, heat clearing away, nourish blood, dispel the wind, manage and wetly can significantly suppress propagation, promote differentiation and reduction ET-1 level for the prescription of the rule of treatment.Again these prescriptions are further optimized afterwards and formed two Chinese medicinal formulaes, carry out zoopery and clinical observation respectively, obtain a clinical and all tangible removing heat from blood and promoting blood circulation compound recipe of experiment effect at last, again this side is used for the psoriatic curative effect of clinical observation treatment, by above-mentioned experiment, utilize the detection means of science to develop the effective Chinese medicinal formulae of clinical treatment psoriasis.
Herbal medicine efficacy is learned the exploitation that detects index
The estrogen phase outgrowth mouse vagina epithelium model of Bonder and Van Scott is used in this research, the Mus tail scale epidermis model of Jarrett and Spearman is as the aggregative indicator (once obtaining prizes such as nineteen ninety State Education Commission's scientific and technological progress 3 3) of psoriasis pharmacodynamics sieve examination, proved that the drug effect sieve examination that above-mentioned experimental model is used for Chinese medicine is feasible, and introduced advanced experimental index and innovate.PCNA is present in the cell of active proliferation, is the best index of reflection cell proliferation on the paraffin section.This research is first with the index of PCNA as the psoriasis experimental model, to observe the influence of medicine on cell proliferation.The result shows that the mouse vagina epithelium of estrogenization is in vegetative state, can truly reflect its propagation degree change from molecular level with the PCNA labelling.It is more directly perceived and reliable to learn method than conventional organization as the index of pharmacodynamic study.Endothelin level-1 (ET-1) has short epidermis propagation in onset of psoriasis, short new vessels forms and the effect of inflammatory cell chemotactic.Being considered to a kind of virulence factor, is a body fluid index of the reflection psoriasis state of an illness.This research has detected the influence of Chinese medicine to blood plasma ET-1 level with putting the method for exempting from.The result shows that these Chinese medicines can significantly reduce mice plasma ET-1 level.Endothelial-leucocyte adhesion mole-cule-E (sE-selectin) is the direct indicator of activated endothelial cell, plays a significant role to the adhesion of circulating leukocyte and to the inflammation part migration.Psoriatic's serum sE-selectin significantly increases, and this experiment is introduced animal model with the sE-selectin horizontal detection as an index first, and the result shows that this index is reliable and stable as a kind of detection means, and easy and simple to handle.This research is explored the detection index that can be applicable to judge herbal medicine efficacy by application and observation to These parameters.Make original experimental model have more science and advance.
Technical solution of the present invention is,
Be used for the treatment of psoriatic removing heat from blood and promoting blood circulation compound recipe, by weight percentage, composition is:
Folium Isatidis 6～12%, the Radix Rehmanniae 6～22%, Radix Scutellariae 4～10%, Radix Arnebiae (Radix Lithospermi) 3～9%, Radix Salviae Miltiorrhizae 5～11%, Radix Paeoniae Rubra 3～7%, Cortex Moutan 4～8%, Radix Angelicae Sinensis 4～16%, Rhizoma Smilacis Glabrae 14～22%, Cortex Dictamni 4～8%, Herba Schizonepetae 2～8%, Flos Lonicerae 8～14%.
Be used for the treatment of psoriatic removing heat from blood and promoting blood circulation compound recipe, by weight percentage, composition is:
Folium Isatidis 9%, the Radix Rehmanniae 18%, Radix Scutellariae 7%, Radix Arnebiae (Radix Lithospermi) 5%, Radix Salviae Miltiorrhizae 7%, Radix Paeoniae Rubra 4%, Cortex Moutan 5%, Radix Angelicae Sinensis 7%, Rhizoma Smilacis Glabrae 18%, Cortex Dictamni 5%, Herba Schizonepetae 4%, Flos Lonicerae 12%.
Preparation is used for the treatment of the method for psoriatic removing heat from blood and promoting blood circulation compound recipe, and step is:
A. the screening of single medicinal material
To treating the side's of tearing open the analysis of psoriatic Chinese medicinal formulae, obtain the higher Chinese medicine of frequency of occurrence, select the effective 20 flavor Chinese medicines of curing psoriasis.
Application can be simulated methods such as psoriatic mouse vagina epithelium and Mus tail scale epidermis model, endothelin level-1 isotope detection, PCNA immunohistochemical staining, serum sE-selectin detection, filters out effective 20 Chinese medicines of curing psoriasis.20 kinds of Chinese medicines are:
The Radix Rehmanniae, Radix Paeoniae Rubra, Cortex Moutan, Radix Arnebiae (Radix Lithospermi), Flos Lonicerae, Folium Isatidis, Rhizoma Smilacis Glabrae, Radix Scutellariae, Radix Sophorae Flavescentis, Cortex Dictamni, Herba Schizonepetae, Radix Salviae Miltiorrhizae, the Radix Astragali, Radix Angelicae Sinensis, Caulis Spatholobi, Rhizoma Chuanxiong, Semen Persicae, Flos Carthami, rhizoma sparganic, Rhizoma Curcumae;
B. distinguish the flavor of Chinese medicines according to the traditional Chinese medical science logos and the rule of treatment to above-mentioned 20, form 6 kinds of folk prescriptions.The function of each side is:
I side: removing heat from blood, heat clearing away
II side: removing heat from blood, invigorate blood circulation, nourish blood
III side: invigorate blood circulation, removing blood stasis with potent drugs
IV side: removing blood stasis with potent drugs, heat clearing away
V side: heat clearing away, dispel the wind, manage wet
VI side: heat clearing away, reason are wet, removing heat from blood, invigorate blood circulation, QI invigorating
Each side is composed as follows:
I side: Folium Isatidis 16～50%, the Radix Rehmanniae 16～50%, Radix Scutellariae 9～26%, Radix Arnebiae (Radix Lithospermi) 9～26%,
II side: Radix Salviae Miltiorrhizae 16～50%, Radix Paeoniae Rubra 8～25%g, Cortex Moutan 8～25%, Radix Angelicae Sinensis 8～24%,
III side: Radix Salviae Miltiorrhizae 20～60%, Radix Angelicae Sinensis 10～30%, Rhizoma Curcumae 10～30%, Rhizoma Chuanxiong 10～30%,
IV side: Semen Persicae 10～30%, rhizoma sparganic 10～30%, Folium Isatidis 20～60%, Flos Carthami 10～30%,
V side: Rhizoma Smilacis Glabrae 33～83%, Cortex Dictamni 6～18%, Herba Schizonepetae 6～18%, Flos Lonicerae 8～32%,
VI side: Radix Sophorae Flavescentis 8～23%, the Radix Astragali 15～60%, Radix Arnebiae (Radix Lithospermi) 8～22%, Caulis Spatholobi 16～46%;
C. form the removing heat from blood and promoting blood circulation compound recipe
Use psoriasis model and observe of the influence of above-mentioned 6 kinds of prescriptions respectively mouse vagina epithelial cell mitosis and the formation of Mus afterbody scale granular layer of epidermis; Application is put the method for exempting from and is detected the influence of 6 kinds of Chinese medicinal formulaes to the mice plasma endothelin-1; To in the above-mentioned experiment epidermis propagation, differentiation and downward modulation blood plasma ET-1 level all be formed the removing heat from blood and promoting blood circulation compound recipe by I, II, the V side of better effect by traditional Chinese medical science logos.
D. the further checking of removing heat from blood and promoting blood circulation compound recipe effect:
The application psoriasis model is observed removing heat from blood respectively, the influence of compound recipe to mouse vagina epithelial cell mitosis and the formation of Mus afterbody scale granular layer of epidermis of invigorating blood circulation.Application is put the method for exempting from and is detected removing heat from blood, the influence of compound recipe to the mice plasma endothelin-1 of invigorating blood circulation.Use ELISA double antibodies sandwich method and survey removing heat from blood, the influence of compound recipe of invigorating blood circulation serum sE-selectin level.Removing heat from blood, the compound recipe of invigorating blood circulation are used for 200 routine psoriasis patients verify its clinical efficacy.
E. it is as follows to finish used medicine, reagent, instrument of above 3 steps:
1. Kunming kind outbreeding system mice
2. injection diethylstilbestrol (Shanghai No.6 Pharmaceutical Factory)
3. injection methotrexate (MTX): 1.0mg/kg (Hualian Pharmaceutical Co., Ltd., Shanghai)
4. colchicine: 2.0mg/kg (Weihui City, Shanghai chemical reagent factory)
5.PCNA monoclonal antibody and HRP/DAB detect box (U.S. NEOMARKERS company)
6.ET-1 put inspection-free survey box (Zong Yuan of PLA is put and exempted from the chamber)
7.ET-1 radioimmunological kit (Zong Yuan of PLA is put and exempted from the chamber)
8.DDL-refrigerated centrifuge (going up Hai'an prosperous scientific instrument factory)
9. γ-12 enumerator (DPC company)
The invention has the beneficial effects as follows, can effectively treat psoriasis, have no side effect, be fit to life-time service.The present invention is from the modern pharmacodynamics R and D treatment by Chinese herbs psoriasis of starting with.Research adopts animal model, SABC, hematology's detection, cell in vitro to cultivate many index and combine, examination of a large amount of zoopery sieve and clinical observation on the therapeutic effect combine, the science detection means and the traditional Chinese medical science logos rule of treatment combine and develop the psoriatic Chinese medicinal formulae of treatment, determined a kind of that be used to develop, science, with the effective technology that abroad integrates with.
The specific embodiment
One, the preparation of removing heat from blood and promoting blood circulation compound recipe
A. select effective 20 Chinese medicines of curing psoriasis:
The Radix Rehmanniae, Radix Paeoniae Rubra, Cortex Moutan, Radix Arnebiae (Radix Lithospermi), Flos Lonicerae, Folium Isatidis, Rhizoma Smilacis Glabrae, Radix Scutellariae, Radix Sophorae Flavescentis, Cortex Dictamni, Herba Schizonepetae, Radix Salviae Miltiorrhizae, the Radix Astragali, Radix Angelicae Sinensis, Caulis Spatholobi, Rhizoma Chuanxiong, Semen Persicae, Flos Carthami, rhizoma sparganic, Rhizoma Curcumae;
B. form 6 kinds of folk prescriptions:
I side: Folium Isatidis 2.5g, Radix Rehmanniae 2.5g, Radix Scutellariae 1.5g, Radix Arnebiae (Radix Lithospermi) 1.5g,
II side: Radix Salviae Miltiorrhizae 2.5g, Radix Paeoniae Rubra 2.0g, Cortex Moutan 2.0g, Radix Angelicae Sinensis 1.5g,
III side: Radix Salviae Miltiorrhizae 2.5g, Radix Angelicae Sinensis 1.5g, Rhizoma Curcumae 1.5g, Rhizoma Chuanxiong 1.5g,
IV side: Semen Persicae 1.5g, rhizoma sparganic 1.5g, Folium Isatidis 2.5g, Flos Carthami 1.5g,
V side: Rhizoma Smilacis Glabrae 10g, Cortex Dictamni 1.5g, Herba Schizonepetae 1.5g, Flos Lonicerae 2.5g,
VI side: Radix Sophorae Flavescentis 1.5g, Radix Astragali 5.0g, Radix Arnebiae (Radix Lithospermi) 1.5g, Caulis Spatholobi 2.5g;
C. form the removing heat from blood and promoting blood circulation compound recipe:
Folium Isatidis 15g, Radix Rehmanniae 15-35g, Radix Scutellariae 9-18g, Radix Arnebiae (Radix Lithospermi) 6-12g, Radix Salviae Miltiorrhizae 12g, Radix Paeoniae Rubra 6g, Cortex Moutan 9g, Radix Angelicae Sinensis 6-16g, Rhizoma Smilacis Glabrae 30g, Cortex Dictamni 9g, Herba Schizonepetae 6g, Flos Lonicerae 15-25g.
Usage: ordinary property of treatment and erythrodermic psoriasis are decocted in water for oral dose one pair of every day.
Two, detect
(1) medicine is to the mitotic influence of mouse vagina epithelial cell
Get female mice, random packet, 10 every group wherein is experimental group regulating YIN and YANG matched group.All mouse peritoneals injection diethylstilbestrol 0.2mg/ day/only, estrogenization is 3 continuously, makes all mouse vagina epitheliums be in vegetative state.Experimental group gives Chinese medicine simultaneously in feminisation and irritates stomach, is respectively single medicinal material, I, II, III, IV, V, VI prescription and compound poria cocos soup group, and continuous 6 days, every day 2 times.Negative control group is given the normal saline lumbar injection.Positive controls is in estrogenization lumbar injection MTX after 3 days, every day 2 times, continuous 3 days.Be the influence to experimental result of the Circadian rhythm of avoiding cell mitogen, all experiment mice last administration times are unified due at 8 o'clock in the morning, the morning 9 o'clock lumbar injection colchicine 2.0mg/kg body weight.Colchicine can't form spindle, and mitosis stops at the interim phase of M so that counting.At 2 o'clock in afternoon was put to death mice, got the vagina specimen with 10% formalin fixed, made the HE section, and light microscopic is counted the mitosis number (mitotic index) in 500 basal cells down, compared with matched group.
(2) medicine is to the influence of mouse tail scale granular layer of epidermis formation
Get male mice, avoid the influence of female endocrine to epidermal differentiation, random packet, is respectively experimental group and positive and negative matched group by 10 every group.Experimental group is irritated stomach with Chinese herbs decoction, every day 2 times, continuous 14 days; Negative control group is with the normal saline lumbar injection; First three day given the MTX lumbar injection, every day 2 times, continuous 2 weeks to positive controls weekly.Put to death mice on the 15th day, get Mus back range root 1.5cm place dorsal part skin, the about 0.3cm * 1.0cm's of size is one rectangular, and the formalin fixed with 10% is made HE and cut into slices, and the scale epidermis of observing each specimen under the light microscopic one by one has or not granular layer formation.Every scale that the granular layer cell of embarking on journey is continuously arranged is judged to be the scale that granular layer forms.Count the scale number that granular layer is arranged in per 100 scales, compare with matched group.
(3) medicine is to the influence of mice plasma ET-1 and serum sE-selectin level
Male mice was got about 1 milliliter of blood with plucking the eyeball method before putting to death, splash in the test tube that is added with EDTA disodium and aprotinin in advance, and 4 ℃ centrifugal, 3000 rev/mins, 10 minutes, got blood plasma and put and exempt from method survey ET-1 content.Get serum ELISA method and detect the sE-selectin concrete operation method: will add buffer and Endothelin antibody in standard substance and each specimen to be measured earlier, mixing was hatched 24 hours for 4 ℃.Add 125 I-Endothelins in each pipe next day, mixing, and 4 ℃ are spent the night.In each pipe, added separating medium respectively on the 3rd day, 125 I-Endothelins of bonding state and free state are separated, measure the sedimentary radioactive intensity of every pipe on γ-calculating instrument, calculate the concentration of Endothelin in every pipe specimen according to standard curve, unit represents with pg/ml.
(4) medicine is to the influence of mouse vagina epithelium PCNA expression
Get female mice, random packet, experimental technique is with the vagina model, but colchicine is not injected in the last administration.The test kit explanation is pressed in PCNA dyeing.Positive control is known expression positive lymph nodes, substitutes an anti-negative control of doing with PBS.The result judges: PCNA stained positive material is the brown yellow granule shape, mainly is positioned at karyon, accidental endochylema.Adopt Wang Hongwei with reference to Garcia[3] method: observe 50 high power fields, the pale brown color dyeing of karyon is positive.Two indexs are observed in each visual field.
A. positive reaction cell proportion:＜20% is 1; 20-50% is 2;＞50% is 3.
B. color depth in positive reaction: majority is shallow in the positive reaction cell is colored as 1, and most dark colored border are clear to be 2.
More than two addition=2 be (+);=3 is (++); 〉=4 be (+++).
Claims (3)
1. be used for the treatment of psoriatic removing heat from blood and promoting blood circulation compound recipe, it is characterized in that, by weight percentage, composition is:
Folium Isatidis 6～12%, the Radix Rehmanniae 6～22%, Radix Scutellariae 4～10%, Radix Arnebiae (Radix Lithospermi) 3～9%, Radix Salviae Miltiorrhizae 5～11%, Radix Paeoniae Rubra 3～7%, Cortex Moutan 4～8%, Radix Angelicae Sinensis 4～16%, Rhizoma Smilacis Glabrae 14～22%, Cortex Dictamni 4～8%, Herba Schizonepetae 2～8%, Flos Lonicerae 8～14%.
2. according to claim 1ly be used for the treatment of psoriatic removing heat from blood and promoting blood circulation compound recipe, it is characterized in that by weight percentage, composition is:
Folium Isatidis 9%, the Radix Rehmanniae 18%, Radix Scutellariae 7%, Radix Arnebiae (Radix Lithospermi) 5%, Radix Salviae Miltiorrhizae 7%, Radix Paeoniae Rubra 4%, Cortex Moutan 5%, Radix Angelicae Sinensis 7%, Rhizoma Smilacis Glabrae 18%, Cortex Dictamni 5%, Herba Schizonepetae 4%, Flos Lonicerae 12%.
3. prepare the described method that is used for the treatment of psoriatic removing heat from blood and promoting blood circulation compound recipe of claim 1, it is characterized in that step is:
A. the screening of single medicinal material
To treating the side's of tearing open the analysis of psoriatic Chinese medicinal formulae, obtain the higher Chinese medicine of frequency of occurrence, use animal model and serology detection method the Chinese medicine of picking out is screened.Obtain the effective 20 flavor Chinese medicines of curing psoriasis:
The Radix Rehmanniae, Radix Paeoniae Rubra, Cortex Moutan, Radix Arnebiae (Radix Lithospermi), Flos Lonicerae, Folium Isatidis, Rhizoma Smilacis Glabrae, Radix Scutellariae, Radix Sophorae Flavescentis, Cortex Dictamni, Herba Schizonepetae, Radix Salviae Miltiorrhizae, the Radix Astragali, Radix Angelicae Sinensis, Caulis Spatholobi, Rhizoma Chuanxiong, Semen Persicae, Flos Carthami, rhizoma sparganic, Rhizoma Curcumae;
B. distinguish the flavor of Chinese medicines according to the traditional Chinese medical science logos and the rule of treatment to above-mentioned 20, form 6 kinds of folk prescriptions
I side: Folium Isatidis 16～50%, the Radix Rehmanniae 16～50%, Radix Scutellariae 9～26%, Radix Arnebiae (Radix Lithospermi) 9～26%,
II side: Radix Salviae Miltiorrhizae 16～50%, Radix Paeoniae Rubra 8～25%g, Cortex Moutan 8～25%, Radix Angelicae Sinensis 8～24%,
III side: Radix Salviae Miltiorrhizae 20～60%, Radix Angelicae Sinensis 10～30%, Rhizoma Curcumae 10～30%, Rhizoma Chuanxiong 10～30%,
IV side: Semen Persicae 10～30%, rhizoma sparganic 10～30%, Folium Isatidis 20～60%, Flos Carthami 10～30%,
V side: Rhizoma Smilacis Glabrae 33～83%, Cortex Dictamni 6～18%, Herba Schizonepetae 6～18%, Flos Lonicerae 8～32%,
VI side: Radix Sophorae Flavescentis 8～23%, the Radix Astragali 15～60%, Radix Arnebiae (Radix Lithospermi) 8～22%, Caulis Spatholobi 16～46%;
C. form the removing heat from blood and promoting blood circulation compound recipe
Use psoriasis model and observe of the influence of above-mentioned 6 kinds of prescriptions respectively mouse vagina epithelial cell mitosis and the formation of Mus afterbody scale granular layer of epidermis; Application is put the method for exempting from and is detected the influence of 6 kinds of Chinese medicinal formulaes to the mice plasma endothelin-1; To in the above-mentioned experiment epidermis propagation, differentiation and downward modulation blood plasma ET-1 level all be formed the removing heat from blood and promoting blood circulation compound recipe by I, II, the V side of better effect by traditional Chinese medical science logos.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101375965B (en) * | 2008-09-27 | 2010-12-15 | 路敦祥 | Chinese medicine honeyed bolus for treating recurrent psoriasis in progressive stage |
| CN102114230A (en) * | 2011-02-21 | 2011-07-06 | 刘绍居 | Medicament for treating psoriasis |
| CN102406893A (en) * | 2011-11-16 | 2012-04-11 | 王莲芬 | Chinese patent medicine for treating psoriasis. |
| CN101385811B (en) * | 2008-10-27 | 2012-07-04 | 严跃华 | Medicament for treating psoriasis |
| CN104096123A (en) * | 2014-08-07 | 2014-10-15 | 范恒银 | External traditional Chinese medicine for treating psoriasis |
| CN110742999A (en) * | 2019-11-29 | 2020-02-04 | 江苏师范大学 | A Chinese medicinal composition for treating psoriasis |
-
2004
- 2004-04-08 CN CN 200410021270 patent/CN1562168A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101375965B (en) * | 2008-09-27 | 2010-12-15 | 路敦祥 | Chinese medicine honeyed bolus for treating recurrent psoriasis in progressive stage |
| CN101385811B (en) * | 2008-10-27 | 2012-07-04 | 严跃华 | Medicament for treating psoriasis |
| CN102114230A (en) * | 2011-02-21 | 2011-07-06 | 刘绍居 | Medicament for treating psoriasis |
| CN102114230B (en) * | 2011-02-21 | 2012-07-04 | 刘绍居 | Medicament for treating psoriasis |
| CN102406893A (en) * | 2011-11-16 | 2012-04-11 | 王莲芬 | Chinese patent medicine for treating psoriasis. |
| CN104096123A (en) * | 2014-08-07 | 2014-10-15 | 范恒银 | External traditional Chinese medicine for treating psoriasis |
| CN110742999A (en) * | 2019-11-29 | 2020-02-04 | 江苏师范大学 | A Chinese medicinal composition for treating psoriasis |
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