CN104523785A - Extraction method of general flavone in honewort - Google Patents

Abstract

The invention discloses an extraction method of general flavone in honeworts, which comprises the following steps: taking honewort stems and leaves, heating the honewort stems and leaves with 80% ethanol with a volume being 8 times of that of the honewort stems and leaves at a temperature of about 80 DEG C to boil, performing reflux with a reflux device for 2 hours, extracting for 3 times, combining the extracts obtained in the three extractions, performing filtration and reduced pressure compression to obtain an extract, diluting the extract with water to obtain a concentrate with a relative density of 1.1 (at a temperature below 20 DEG C), filtering the concentrate, applying the concentrate to a polyamide column with an application volume being about 70% of the column volume, standing and adsorbing for 4 hours, eluting with water, 10% ethanol, and 70% ethanol in order with volumes being three times of the volume of the column, performing reduced pressure concentration to obtain a dry product, and drying the product at 105 DEG C for about 24 hours, crushing, and sieving with a 60-mesh sieve to obtain the product. The method of the invention realizes effective extraction of general flavone in honeworts through several steps, and is high in extraction efficiency and less in raw material waste.

Description

The extracting method of total flavones in a kind of Radix Bupleuri

Technical field

The present invention relates to the extract drugs method in a kind of plant, particularly relate to the extracting method of total flavones in a kind of Radix Bupleuri.

Background technology

Radix Bupleuri is called: HERBA BUPLEURI (Zhiwu Mingshi Tukao), hard Seedling Radix Bupleuri (northeast medicinal plants figure will), B. kunmingense Y. Li et S. L. Pan (Anhui), Tianjin Radix Bupleuri etc.Be born in the places such as drier hillside, field and roadside.Be distributed in: Northeast China, North China, East China, Central-South, the southwestern and ground such as Shaanxi, Gansu.Bupleurum perennial herb medicinal plants, needs within 2 years, to complete a growth promoter cycle.The growth of artificial culture Radix Bupleuri First Year is raw basal leaf and stem only, only has little plant to open a small amount of flower, still can not produce seed.Field can overwintering.Second Year is turned green spring, and plant strain growth is rapid, and bloom in 7 ~ JIUYUE, 8 ~ October is fructescence.About 190 ~ 200 days time of infertility.In each organ of Radix Bupleuri, the amount of total flavones is very abundant, and the Flavonoid substances in Radix Bupleuri is mainly accumulated in its stem and leaf and genitals, and the amount in root is little.In the growth course of stem and leaf, the total flavones amount of full-bloom stage is the highest, and amount during setting declines to some extent, and amount during fruit maturation starts again to rise, and when withered early stage, the total flavones amount in both returns to again a higher level.How to carry out extraction to the total flavones in Radix Bupleuri is a research emphasis.

Summary of the invention

Object of the present invention is just the extracting method providing total flavones in a kind of Radix Bupleuri in order to solve the problem.

The present invention is achieved through the following technical solutions above-mentioned purpose:

An extracting method for total flavones in Radix Bupleuri, comprises the following steps:

(1) get Radix Bupleuri stem and leaf, employing cumulative volume is the content of Radix Bupleuri stem and leaf 8 times is the ethanol of 80%, at the heating temperatures of about 80 degree to boiling, adopts reflux, refluxes 2 hours, extract 3 times altogether, enter next step;

(2) extracting solution extracted three times merges, and filter, compression of reducing pressure obtains extractum, extractum is diluted with water to the concentrated solution (in 20 degrees Celsius of situations) of relative density about 1.1, enters next step;

(3) filtered by concentrated solution, upper polyamide column, loading volume is about 0.7 column volume, standing adsorption 4 hours, use the water of 3 times of column volumes successively, the ethanol of 10%, the ethanol elution of 70%, collect 70% ethanol elution, be evaporated to dry, enter next step;

(4) dry 24 hours under 105 degree celsius temperature, cross 60 mesh sieves after crushed and both obtained.

Beneficial effect of the present invention is:

The invention provides the extracting method of total flavones in a kind of Radix Bupleuri, achieved by multiple step and effectively extract the total flavones in Radix Bupleuri, extraction ratio is high, and wastage of material is less, has the value promoted the use of.

Detailed description of the invention

The invention will be further described below:

An extracting method for total flavones in Radix Bupleuri, comprises the following steps:

(1) get Radix Bupleuri stem and leaf, employing cumulative volume is the content of Radix Bupleuri stem and leaf 8 times is the ethanol of 80%, at the heating temperatures of about 80 degree to boiling, adopts reflux, refluxes 2 hours, extract 3 times altogether, enter next step;

(2) extracting solution extracted three times merges, and filter, compression of reducing pressure obtains extractum, extractum is diluted with water to the concentrated solution (in 20 degrees Celsius of situations) of relative density about 1.1, enters next step;

(3) filtered by concentrated solution, upper polyamide column, loading volume is about 0.7 column volume, standing adsorption 4 hours, use the water of 3 times of column volumes successively, the ethanol of 10%, the ethanol elution of 70%, collect 70% ethanol elution, be evaporated to dry, enter next step;

(4) dry 24 hours under 105 degree celsius temperature, cross 60 mesh sieves after crushed and both obtained.

Technical scheme of the present invention is not limited to the restriction of above-mentioned specific embodiment, the technology distortion that every technical scheme according to the present invention is made, and all falls within protection scope of the present invention.

Claims (1)

1. the extracting method of total flavones in Radix Bupleuri, is characterized in that, comprise the following steps:

(1) get Radix Bupleuri stem and leaf, employing cumulative volume is the content of Radix Bupleuri stem and leaf 8 times is the ethanol of 80%, at the heating temperatures of about 80 degree to boiling, adopts reflux, refluxes 2 hours, extract 3 times altogether, enter next step;

(2) extracting solution extracted three times merges, and filter, compression of reducing pressure obtains extractum, extractum is diluted with water to the concentrated solution (in 20 degrees Celsius of situations) of relative density about 1.1, enters next step;

(3) filtered by concentrated solution, upper polyamide column, loading volume is about 0.7 column volume, standing adsorption 4 hours, use the water of 3 times of column volumes successively, the ethanol of 10%, the ethanol elution of 70%, collect 70% ethanol elution, be evaporated to dry, enter next step;

(4) dry 24 hours under 105 degree celsius temperature, cross 60 mesh sieves after crushed and both obtained.