CN104511031B - Cecropin antibacterial peptide fermentation sterilization equipment and sterilization method thereof - Google Patents
Cecropin antibacterial peptide fermentation sterilization equipment and sterilization method thereof Download PDFInfo
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- CN104511031B CN104511031B CN201310450659.2A CN201310450659A CN104511031B CN 104511031 B CN104511031 B CN 104511031B CN 201310450659 A CN201310450659 A CN 201310450659A CN 104511031 B CN104511031 B CN 104511031B
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- 230000001954 sterilising effect Effects 0.000 title claims abstract description 77
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 22
- 108050004290 Cecropin Proteins 0.000 title claims abstract description 19
- 238000000855 fermentation Methods 0.000 title claims abstract description 19
- 230000004151 fermentation Effects 0.000 title claims abstract description 19
- 239000003910 polypeptide antibiotic agent Substances 0.000 title abstract description 5
- 239000007788 liquid Substances 0.000 claims abstract description 26
- 238000003756 stirring Methods 0.000 claims abstract description 12
- 238000009434 installation Methods 0.000 claims description 11
- 208000033999 Device damage Diseases 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
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- Apparatus For Disinfection Or Sterilisation (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fertilizers (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention provides cecropin antibacterial peptide fermentation sterilization equipment and a sterilization method thereof. The equipment includes a sterilization tank and a stirrer mounted in the sterilization tank, three steam pipelines are disposed at the bottom of the sterilization tank, and one of the steam pipelines is connected to an air pipeline. The invention also includes a sterilization method. According to the invention, one steam pipeline is provided with the air pipeline so that air can enter at the same time, steam and air are fed simultaneously, air makes the steam disperse rapidly, a feed liquid can be heated evenly, sound and vibration can be reduced to avoid device damage, and also influence to the work environment is reduced. According to the invention, when the feed liquid is heated to 110DEG C, stirring can be stopped, and electric energy is saved.
Description
Technical field
The present invention relates to biological fermentation field, and in particular to a kind of cecropin antimicrobial peptides fermentation and sterilization equipment and its sterilizing side
Method.
Background technology
Cecropin antimicrobial peptides are first Antibacterial Peptide From Animals being found, and 1980, are separated from day Pupa bombycises by Boman etc.
Obtain.The features such as this kind of active polypeptide majority has strong basicity, heat stability and broad-spectrum antiseptic, antibacterial peptide has very strong to antibacterial
Lethal effect, especially its killing action to some drug resistance pathogen more cause the attention of people.
When antibacterial peptide fermenting and producing sterilizes at present, it is directly to lead to high-temperature steam toward inside sterilization tank to be sterilized, due to firm
During beginning, than relatively low, vapor (steam) temperature is high for feed temperature, and Temperature Difference Ratio is larger, some feed liquid quick heatings, and some intensifications are slow, and feed liquid is heated
It is uneven, huge sound and vibrations can be produced, it is big to sterilizing installation damage comparison, also affect working environment.
The content of the invention
The present invention gives birth to the problem of huge sound and vibrations when sterilizing for above-mentioned existing feed liquid, there is provided a kind of cecropin resists
Bacterium peptide fermentation and sterilization equipment and its sterilizing methods, will not produce strong sound and vibrations, protection equipment during its sterilizing.
The technical scheme that the present invention takes is：
A kind of cecropin antimicrobial peptides fermentation and sterilization equipment, including sterilization tank and the agitator in sterilization tank, sterilizing
Pot bottom is provided with three jet chimneys, is connected with air line on one of jet chimney.
Control valve is provided with the air line.
A kind of method for carrying out cecropin antimicrobial peptides fermentation and sterilization using above-mentioned sterilizing installation, comprises the steps：
（1）Air, steam sterilization：After feed liquid enters sterilization tank, be connected with the jet chimney of air line and meanwhile enter steam and
Air, steam input 550-650 m3/ hour, air input are 150-250m3/ hour；Another two jet chimney list enters steam,
Steam input 750-850 m3/ hour, vapor (steam) temperature are 135 DEG C -145 DEG C, while being stirred；
（2）When feed temperature is heated to 85-95 DEG C, stop into air；
（3）When feed temperature is heated to 105-115 DEG C, stop stirring；
（4）Feed liquid is heated to into 115-125 DEG C, is sterilized 25-35 minutes.
A kind of method for carrying out cecropin antimicrobial peptides fermentation and sterilization using above-mentioned sterilizing installation, comprises the steps：
（1）, air, steam sterilization：After feed liquid enters sterilization tank, be connected with the jet chimney of air line and meanwhile enter steam and
Air, 600 m of steam input3/ hour, air input are 200m3/ hour；Another two jet chimney list enters steam, steam input
800 m3/ hour, vapor (steam) temperature are 135 DEG C -145 DEG C, while being stirred；
（2）, when feed temperature is heated to 90 DEG C, stop into air；
（3）, when feed temperature is heated to 110 DEG C, stop stirring；
（4）, feed liquid is heated to into 120 DEG C, sterilize 30 minutes.
The present invention is provided with air line on a jet chimney, can enter air simultaneously, enter to steam during present invention sterilizing simultaneously
Vapour and air, air make steam disperse rapidly so that feed liquid is heated evenly, and reduce sound and vibrations, it is to avoid damage equipment, also subtract
The impact to working environment is lacked, the present invention is heated to 110 DEG C in feed temperature, just can stop stirring, save electric energy.
Description of the drawings
Fig. 1 is schematic structural view of the invention.
Specific embodiment
As shown in figure 1, a kind of cecropin antimicrobial peptides fermentation and sterilization equipment, including sterilization tank 1 and in sterilization tank 1
Agitator 5,1 bottom of sterilization tank are provided with three jet chimneys 2, and air line 3, air are connected with one of jet chimney
Control valve 4 is provided with pipeline 3.
Sterilizing methods embodiment 1：A kind of method for carrying out cecropin antimicrobial peptides fermentation and sterilization using above-mentioned sterilizing installation,
Comprise the steps：
（1）, air, steam sterilization：After feed liquid enters sterilization tank, air line is connected with air compressor, is connected with air
The jet chimney of pipeline enters steam and air, 600 m of steam input simultaneously3/ hour, air input are 200m3/ hour；Another two
Jet chimney list enters steam, 800 m of steam input3/ hour, vapor (steam) temperature be 135 DEG C -145 DEG C, can for 135 DEG C, 138 DEG C,
140 DEG C, 143 DEG C or 150 DEG C, i.e. vapor (steam) temperature can be arbitrary value between 135 DEG C -145 DEG C, while turn on agitator is stirred
Mix；
（2）, when feed temperature is heated to 90 DEG C, stop into air because after 90 DEG C, feed temperature and the steam temperature difference
It is smaller, huge sound and vibrations will not be produced；
（3）, when feed temperature is heated to 110 DEG C, stop stirring, save electric energy；
（4）, feed liquid is heated to into 120 DEG C, sterilize 30 minutes.
Sterilizing methods embodiment 2：A kind of method for carrying out cecropin antimicrobial peptides fermentation and sterilization using above-mentioned sterilizing installation, bag
Include following steps：
（1）Air, steam sterilization：After feed liquid enters sterilization tank, be connected with the jet chimney of air line and meanwhile enter steam and
Air, steam input 550m3/ hour, air input are 150m3/ hour；Another two jet chimney list enters steam, steam input
750 m3/ hour, vapor (steam) temperature are 135 DEG C, while being stirred；
（2）When feed temperature is heated to 85 DEG C, stop into air；
（3）When feed temperature is heated to 105 DEG C, stop stirring；
（4）Feed liquid is heated to into 115 DEG C, is sterilized 25 minutes.
Sterilizing methods embodiment 3：A kind of above-mentioned sterilizing installation of exploitation right carries out the side of cecropin antimicrobial peptides fermentation and sterilization
Method, comprises the steps：
（1）Air, steam sterilization：After feed liquid enters sterilization tank, be connected with the jet chimney of air line and meanwhile enter steam and
Air, 650 m of steam input3/ hour, air input are 250m3/ hour；Another two jet chimney list enters steam, steam input
850 m3/ hour, vapor (steam) temperature are 145 DEG C, while being stirred；
（2）When feed temperature is heated to 95 DEG C, stop into air；
（3）When feed temperature is heated to 115 DEG C, stop stirring；
（4）Feed liquid is heated to into 125 DEG C, is sterilized 35 minutes.
Sterilizing methods embodiment 4：A kind of method for carrying out cecropin antimicrobial peptides fermentation and sterilization using above-mentioned sterilizing installation,
Comprise the steps：
（1）Air, steam sterilization：After feed liquid enters sterilization tank, be connected with the jet chimney of air line and meanwhile enter steam and
Air, 580 m of steam input3/ hour, air input are 180m3/ hour；Another two jet chimney list enters steam, steam input
790 m3/ hour, vapor (steam) temperature are 139 DEG C, while being stirred；
（2）When feed temperature is heated to 91 DEG C, stop into air；
（3）When feed temperature is heated to 108 DEG C, stop stirring；
（4）Feed liquid is heated to into 119 DEG C, is sterilized 29 minutes.
Sterilizing methods embodiment 5：A kind of above-mentioned sterilizing installation of utilization right carries out the side of cecropin antimicrobial peptides fermentation and sterilization
Method, comprises the steps：
（1）Air, steam sterilization：After feed liquid enters sterilization tank, be connected with the jet chimney of air line and meanwhile enter steam and
Air, 620 m of steam input3/ hour, air input are 220m3/ hour；Another two jet chimney list enters steam, steam input
840 m3/ hour, vapor (steam) temperature are 142 DEG C, while being stirred；
（2）When feed temperature is heated to 92 DEG C, stop into air；
（3）When feed temperature is heated to 110 DEG C, stop stirring；
（4）Feed liquid is heated to into 111 DEG C, is sterilized 31 minutes.
Sterilizing methods embodiment 6：A kind of above-mentioned sterilizing installation of utilization right carries out cecropin antimicrobial peptides fermentation and sterilization
Method, comprises the steps：
（1）Air, steam sterilization：After feed liquid enters sterilization tank, be connected with the jet chimney of air line and meanwhile enter steam and
Air, 650 m of steam input3/ hour, air input are 250m3/ hour；Another two jet chimney list enters steam, steam input
850 m3/ hour, vapor (steam) temperature are 145 DEG C, while being stirred；
（2）When feed temperature is heated to 95 DEG C, stop into air；
（3）When feed temperature is heated to 115 DEG C, stop stirring；
（4）Feed liquid is heated to into 125 DEG C, is sterilized 35 minutes.
Claims (2)
1. a kind of cecropin antimicrobial peptides fermentation and sterilization equipment, including sterilization tank (1) and the agitator being arranged in sterilization tank (1)
(5), sterilization tank (1) bottom is provided with three jet chimneys (2), it is characterised in that:Connect on one of jet chimney free
Feed channel (3), is provided with control valve (4) on the air line (3)；
The method that cecropin antimicrobial peptides fermentation and sterilization is carried out using the sterilizing installation, including
Following steps:
(1) air, steam sterilization:After feed liquid enters sterilization tank, the jet chimney of air line is connected with while entering steam and air,
Steam input 550-650 m3/ hour, air input are 150-250m3/ hour;Another two jet chimney list enters steam, and steam enters
Amount 750-850 m3/ hour, vapor (steam) temperature are 135 DEG C -145 DEG C, while being stirred;
(2) when feed temperature is heated to 85-95 DEG C, stop into air;
(3) when feed temperature is heated to 105-115 DEG C, stop stirring;
(4) feed liquid is heated to into 115-125 DEG C, is sterilized 25-35 minutes.
2. the method for carrying out cecropin antimicrobial peptides fermentation and sterilization using the sterilizing installation described in claim 1, comprises the steps:
(1) air, steam sterilization:After feed liquid enters sterilization tank, the jet chimney of air line is connected with while entering steam and air,
Steam input 600m3/ hour, air input are 200m3/ hour;Another two jet chimney list enters steam, steam input 800m3/
Hour, vapor (steam) temperature is 135 DEG C -145 DEG C, while being stirred;
(2) when feed temperature is heated to 90 DEG C, stop into air;
(3) when feed temperature is heated to 110 DEG C, stop stirring;
(4) feed liquid is heated to into 120 DEG C, is sterilized 30 minutes.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310450659.2A CN104511031B (en) | 2013-09-29 | 2013-09-29 | Cecropin antibacterial peptide fermentation sterilization equipment and sterilization method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310450659.2A CN104511031B (en) | 2013-09-29 | 2013-09-29 | Cecropin antibacterial peptide fermentation sterilization equipment and sterilization method thereof |
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| CN104511031A CN104511031A (en) | 2015-04-15 |
| CN104511031B true CN104511031B (en) | 2017-04-19 |
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| MA45482A (en) * | 2017-06-01 | 2019-02-20 | Takase Tech Co Ltd | FUEL PRODUCTION PROCESS AND FUEL PRODUCTION DEVICE |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008105696A1 (en) * | 2007-03-01 | 2008-09-04 | Getinge Sterilization Ab | Sterilization device, sterilization process, vaporizing system and use of such a vaporizing system |
| CN101974409A (en) * | 2010-11-27 | 2011-02-16 | 福州大北农生物技术有限公司 | Bacterial culture fermentation tank device and fermentation technology thereof |
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2013
- 2013-09-29 CN CN201310450659.2A patent/CN104511031B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008105696A1 (en) * | 2007-03-01 | 2008-09-04 | Getinge Sterilization Ab | Sterilization device, sterilization process, vaporizing system and use of such a vaporizing system |
| CN101974409A (en) * | 2010-11-27 | 2011-02-16 | 福州大北农生物技术有限公司 | Bacterial culture fermentation tank device and fermentation technology thereof |
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| CN104511031A (en) | 2015-04-15 |
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Effective date of registration: 20160804 Address after: 456550, No. 8 Luban Road, Linzhou industrial agglomeration area, Anyang, Henan, Linzhou Applicant after: LINZHOU SINAGRI YINGTAI BIOLOGICAL PEPTIDES CO.,LTD. Address before: 456550 No. 8 Luban Road, Linzhou industrial agglomeration, Anyang, Henan Applicant before: SINAGRI YINTHAI LINZHOU BIOLOGICAL PARK CO.,LTD. |
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Denomination of invention: The invention relates to a fermentation and sterilization equipment for cecropin antibacterial peptide and a sterilization method thereof Effective date of registration: 20200812 Granted publication date: 20170419 Pledgee: Anyang Investment Group Co.,Ltd. Pledgor: LINZHOU SINAGRI YINGTAI BIOLOGICAL PEPTIDES Co.,Ltd. Registration number: Y2020980004904 |
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Date of cancellation: 20220718 Granted publication date: 20170419 Pledgee: Anyang Investment Group Co.,Ltd. Pledgor: LINZHOU SINAGRI YINGTAI BIOLOGICAL PEPTIDES CO.,LTD. Registration number: Y2020980004904 |
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Denomination of invention: A fermentation sterilization equipment and sterilization method for Tianchansu antibacterial peptide Effective date of registration: 20230414 Granted publication date: 20170419 Pledgee: Anyang Investment Group Co.,Ltd. Pledgor: LINZHOU SINAGRI YINGTAI BIOLOGICAL PEPTIDES CO.,LTD. Registration number: Y2023980038083 |