CN104509825A - Extraction method of natural lotus-seed extract - Google Patents
Extraction method of natural lotus-seed extract Download PDFInfo
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- CN104509825A CN104509825A CN201410822229.3A CN201410822229A CN104509825A CN 104509825 A CN104509825 A CN 104509825A CN 201410822229 A CN201410822229 A CN 201410822229A CN 104509825 A CN104509825 A CN 104509825A
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- seed extract
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Abstract
The invention discloses an extraction method of natural lotus-seed extract. The extraction method of the natural lotus-seed extract comprises enzymolysis and extraction of ground lotus-seed particles. The natural lotus-seed extract prepared with the extraction method can serve as a food additive, can better improve the taste and the aroma of products using the natural lotus-seed extract and enables the aroma to be richer; compared with traditional synthesized lotus-seed essence, the natural lotus-seed extract has more real aroma. The natural lotus-seed extract completely comprises natural compositions, solves a food safety problem caused by traditional synthesized essence additives and has good application value.
Description
Technical field
The present invention relates to essence and flavoring agent manufacture field, be specifically related to that a kind of composition is natural, the extracting method of fragrance and all good lotus seeds natural extract of fragrance.
Background technology
At present, the monomer perfume material of the raw materials for production of tradition lotus seeds essence mainly some Prof. Du Yucang, although the lotus seeds essence using these perfume materials to produce has certain lotus seeds local flavor, but the use of synthesis perfume material have impact on the natural flavor of lotus seeds in food, compared with true lotus seeds fragrance, there is natural sense strong not, the shortcomings such as fragrance stability is not good, and the main perfume material chemical synthesis product often that tradition uses, certain chemical odor can be produced, the not natural sense of tool, cause fragrance fragrance effects bad, liking not by consumer, the application of product is subject to certain restrictions.
Summary of the invention
The object of the invention is to the shortcoming making up above-mentioned traditional lotus seeds essence, the extracting method of the lotus seeds natural extract providing a kind of natural, fragrance and fragrance all to strengthen.
Above-mentioned purpose of the present invention is achieved by the following technical programs:
An extracting method for lotus seeds natural extract, comprises the steps:
(1) lotus nut starch of peeling belt carcass is broken to 60 ~ 80 orders;
(2) 1000g lotus seeds pulverized particles is taken, add 4000g distilled water, with 1 ~ 3g endoglucanase (Novi's letter) and 1g pectase (Novi's letter) enzymolysis 2h, then use 2 ~ 5g glucoamylase (Novi's letter) enzymolysis 2h, obtain enzymolysis head product;
(3) enzymolysis head product is placed in Rotary Evaporators, 40 ~ 50 DEG C, under-0.08 ~-0.1Mpa condition till evaporating water;
(4) join in extraction tank after the enzymolysis head product after evaporating water and ethanol being mixed by 3:1 mass ratio, at 50 ~ 60 DEG C, static extracting 2h under 35 ~ 40Mpa condition;
(5) control CO
2flow is at 10 ~ 20L/h, and 45 ~ 60 DEG C, start dynamic extraction under 30 ~ 35Mpa condition, extraction time is 3h, collects Semen Nelumbinis extract;
(6) remove the ethanol in Semen Nelumbinis extract by rotary evaporation, the condition of rotary evaporation is 35 ~ 45 DEG C ,-0.08 ~-0.1Mpa, obtains lotus seeds natural extract.
Lotus seeds natural extract of the present invention as food additives, can add in the food such as all kinds of Chinese style, western-style cake, bread, particularly can add in Chinese style moon cake.
Compared with prior art, the present invention has following beneficial effect:
(1) preparation method of the present invention ensure that the natural attribute of lotus seeds raw material.The Semen Nelumbinis extract extracted according to the method for the invention can strengthen mouthfeel and the fragrance of its application product better, wherein interpolation red bean and mung bean natural extract can make fragrance fuller, compared with conventional synthesis lotus seeds essence, fragrance is more true, be common use synthetic perfume allocate the lotus seeds essence obtained inaccessiable.
(2) lotus seeds natural extract of the present invention all adopts natural component, solves the food-safety problem of traditional synthetic perfume additive.
(3) lotus seeds natural extract of the present invention adds in Chinese pastry, western-style cake or bakery, thus the lotus seeds fragrance increased in these food, and lotus seeds fragrance and the mouthfeel of natural sense can be had more for product tape, make the heat resistance of product, aroma quality and stability higher than other like products simultaneously.
Detailed description of the invention
Explain the present invention further below in conjunction with specific embodiment, but embodiment does not limit in any form to the present invention.Raw material involved by following examples, preparation method etc., if no special instructions, all can be regarded as the raw material of this area routine, preparation method.
Embodiment 1
(1) lotus nut starch of peeling belt carcass is broken to 60 orders;
(2) 1000g lotus seeds pulverized particles is taken, add 4000g distilled water, with 1g endoglucanase (Novi's letter) and 1g pectase (Novi's letter) enzymolysis 2h, then use 2g glucoamylase (Novi's letter) enzymolysis 2h, obtain enzymolysis head product;
(3) enzymolysis head product is placed in Rotary Evaporators, 40 DEG C, under-0.08Mpa condition till evaporating water;
(4) join in extraction tank after the enzymolysis head product after evaporating water and ethanol being mixed by 3:1 mass ratio, at 50 DEG C, static extracting 2h under 35Mpa condition;
(5) control CO
2flow is at 10L/h, and 45 DEG C, start dynamic extraction under 30Mpa condition, extraction time is 3h, collects Semen Nelumbinis extract;
(6) remove the ethanol in Semen Nelumbinis extract by rotary evaporation, the condition of rotary evaporation is 35 DEG C ,-0.08Mpa, obtains lotus seeds natural extract 16g.
Embodiment 2
(1) lotus nut starch of peeling belt carcass is broken to 80 orders;
(2) 1000g lotus seeds pulverized particles is taken, add 4000g distilled water, with 3g endoglucanase (Novi's letter) and 1g pectase (Novi's letter) enzymolysis 2h, then use 5g glucoamylase (Novi's letter) enzymolysis 2h, obtain enzymolysis head product;
(3) enzymolysis head product is placed in Rotary Evaporators, 50 DEG C, under-0.1Mpa condition till evaporating water;
(4) join in extraction tank after the enzymolysis head product after evaporating water and ethanol being mixed by 3:1 mass ratio, at 60 DEG C, static extracting 2h under 40Mpa condition;
(5) control CO
2flow is at 20L/h, and 60 DEG C, start dynamic extraction under 35Mpa condition, extraction time is 3h, collects Semen Nelumbinis extract;
(6) remove the ethanol in Semen Nelumbinis extract by rotary evaporation, the condition of rotary evaporation is 45 DEG C ,-0.1Mpa, obtains lotus seeds natural extract 15g.
Experimental example 1
Comment examination marking through 15 experts respectively to the fragrance of embodiment 1 and 2 gained lotus seed food additive and mouthfeel, full marks 10 points, average, result is as shown in table 1.
Table 1
| Embodiment 1 | Embodiment 2 | |
| Fragrance | 8.2 | 8.0 |
| Mouthfeel | 8.5 | 8.2 |
Experimental example 2
Conventionally make moon cake.Experiment is divided into three groups, be respectively control group (using lotus-seed paste fillings), experimental group 1 (uses the lotus seeds natural extract that embodiment 1 is obtained, use amount is 0.1% of the quality of control group lotus-seed paste fillings), and experimental group 2 (use the lotus seeds natural extract that embodiment 1 is obtained, use amount is 0.3% of the quality of control group lotus-seed paste fillings).
Please the specialty personnel that participate in evaluation and electing attempt respectively judging for the moon cake of aforementioned three obtained groups, result is as shown in table 2.
Table 2
Result shows, Semen Nelumbinis extract interpolation 0.3% and 0.1% fragrance and mouthfeel are all than not adding the good of lotus seeds natural extract, the personnel that participate in evaluation and electing are suitable to the fancy grade of two lotus seeds natural extract interpolation groups, and it is 0.3% lotus seeds natural extract one group that the sweet trencherman of preference understands preference addition.
Comparative example 1
Experiment is divided into four groups, be respectively experimental group 1 (the commercially available lotus seed paste mooncake in wineshop, Guangzhou), experimental group 2 (lotus seed paste mooncake is sold in the fragrant real estate market of lotus), experimental group 3 (the commercially available lotus seed paste mooncake of high honour), experimental group 4 (conventionally make moon cake, wherein with the addition of embodiment 1 gained lotus seeds natural extract).
Please 15 specialty personnel that participate in evaluation and electing attempt respectively judging for the moon cake of above-mentioned four groups, result is as shown in table 4.Wherein, standards of grading with commercially available lotus-seed paste fillings (manufacturer is the tasty and refreshing good fortune in wineshop, Guangzhou, and product is called pure white lotus-seed paste fillings) for benchmark (fragrance 5 points.Mouthfeel 5 points), best result is 10 points.
Table 3
Comparative example 2
Existing extracting method (control group):
(1) get 1000g lotus nut starch, add 5000g distilled water, electricity consumption mixer 500rpm stirs, and adds lemon acid for adjusting pH to 3.8 to 4.5, adds 0.1% acidic cellulase, adds 0.2% pectase and 0.1% compound protein enzyme reaction 1h after reaction 1h.
(2) product in step (1) is transferred in fermentation tank, adds the glucose of 1%, the yeast extract of 1%, the tryptone of 1%.And regulate pH to 6.0 with 1mol/L phosphate buffer, be heated to 80 DEG C of deactivations after reaction 24h.
(3) enzymatic hydrolysis and fermentation product carries out constant flow filtering or utilizes ceramic membrane filter in sheet frame, concentrates after filtration under 0.1Mpa pressure in 25L Rotary Evaporators, adds 7% alcohol and namely obtain Semen Nelumbinis extract 10g after concentrated 1000 times.
Experimental group method is as embodiment 1.
In table, the mean value that yield draws after being through and repeating to extract experiment for 5 times; Fragrance mouthfeel is the average mark that 15 specialties comment examination personnel to get; Tolerable temperature then with lasting rate for benchmark, experimental group lasting rate when 150 DEG C of 60min is the lasting rate of 85%, 200 DEG C of 30min is 68%.Control group 130 DEG C, 60min time lasting rate be 78%; The lasting rate of 180 DEG C of 30min is 50%.From result, the tolerable temperature of extracting method products obtained therefrom of the present invention is good compared with the tolerable temperature of traditional extraction process products obtained therefrom, and lasting rate significantly improves.
Claims (1)
1. an extracting method for lotus seeds natural extract, is characterized in that comprising the steps:
(1) lotus nut starch of peeling belt carcass is broken to 60 ~ 80 orders;
(2) take 1000g lotus seeds pulverized particles, add 4000g distilled water, with 1 ~ 3g endoglucanase and 1g pectinase enzymatic hydrolysis 2h, then use 2 ~ 5g glucoamylase enzymolysis 2h, obtain enzymolysis head product;
(3) enzymolysis head product is placed in Rotary Evaporators, 40 ~ 50 DEG C, under-0.08 ~-0.1Mpa condition till evaporating water;
(4) join in extraction tank after the enzymolysis head product after evaporating water and ethanol being mixed by 3:1 mass ratio, at 50 ~ 60 DEG C, static extracting 2h under 35 ~ 40Mpa condition;
(5) control CO
2flow is at 10 ~ 20L/h, and 45 ~ 60 DEG C, start dynamic extraction under 30 ~ 35Mpa condition, extraction time is 3h, collects Semen Nelumbinis extract;
(6) remove the ethanol in Semen Nelumbinis extract by rotary evaporation, the condition of rotary evaporation is 35 ~ 45 DEG C ,-0.08 ~-0.1Mpa, obtains lotus seeds natural extract.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109247558A (en) * | 2018-08-25 | 2019-01-22 | 上海华宝孔雀香精有限公司 | A kind of natural red bayberry essence and its extraction process |
| CN109832615A (en) * | 2019-01-04 | 2019-06-04 | 中国食品发酵工业研究院有限公司 | A kind of lotus seeds fermented product and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1724005A (en) * | 2005-07-18 | 2006-01-25 | 天津大学 | Method for extracting volatile oil and liposoluble alkaloid from plumula nelumbini |
| CN103340453A (en) * | 2013-07-08 | 2013-10-09 | 长沙理工大学 | Method for producing low-sugar low-fat lotus paste through enzymolysis processing |
| CN103393163A (en) * | 2013-07-08 | 2013-11-20 | 长沙理工大学 | Method for producing lotus paste by lotus seed buffing powder |
| KR20140019585A (en) * | 2012-08-06 | 2014-02-17 | 대한민국(보건복지부장관) | Cosmetic composition comprising yeonwoojuanbang and yeonbang for skin whitening |
-
2014
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1724005A (en) * | 2005-07-18 | 2006-01-25 | 天津大学 | Method for extracting volatile oil and liposoluble alkaloid from plumula nelumbini |
| KR20140019585A (en) * | 2012-08-06 | 2014-02-17 | 대한민국(보건복지부장관) | Cosmetic composition comprising yeonwoojuanbang and yeonbang for skin whitening |
| CN103340453A (en) * | 2013-07-08 | 2013-10-09 | 长沙理工大学 | Method for producing low-sugar low-fat lotus paste through enzymolysis processing |
| CN103393163A (en) * | 2013-07-08 | 2013-11-20 | 长沙理工大学 | Method for producing lotus paste by lotus seed buffing powder |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109247558A (en) * | 2018-08-25 | 2019-01-22 | 上海华宝孔雀香精有限公司 | A kind of natural red bayberry essence and its extraction process |
| CN109247558B (en) * | 2018-08-25 | 2022-08-09 | 上海华宝孔雀香精有限公司 | Natural waxberry essence and extraction process thereof |
| CN109832615A (en) * | 2019-01-04 | 2019-06-04 | 中国食品发酵工业研究院有限公司 | A kind of lotus seeds fermented product and preparation method thereof |
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Address after: 510760 Guangdong city of Guangzhou Province Economic and Technological Development Zone East Wang Ming Road No. 139 Patentee after: Guangzhou Alchem Biotechnology Co., Ltd. Address before: 510760 Guangdong city of Guangzhou Province Economic and Technological Development Zone East Wang Ming Road No. 139 Patentee before: Guangzhou Aojian Fengze Biological Technology Co.,Ltd. |